Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,784,462 G→T 9.7% R248L (CGG→CTG)  nikE → nickel ABC transporter ATP‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,784,4620GT 9.7% 122.2 / 2.2 41R248L (CGG→CTG) nikEnickel ABC transporter ATP‑binding protein
Reads supporting (aligned to +/- strand):  ref base G (19/18);  new base T (2/2);  total (21/20)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01

CAGCGCAGCGGCACCGCTTTCGTGCTGATCAGCCATGACCTGAGCCTGGTGCAGTACTTCTGCCAGCGGGTTGTGCTGCTGCACGGCGGGCGCATCGTGGAGGAACGCCCCGTCACGCACGACCTGTGCTTTGCGCACCCCGTGGGCCGGCAGCTGCAGGCGGCGGTGTTGCCTGTGCGCCCGCAGAGGAGGCCATCACCGCAAGGCCTGCCAACAGCTGCACACTGACACCACAGACTGGCTTTCGGATCGTCGGAAAACAGGCTATGTTCACCAGACTGATCTGCG  >  NC_002947/3784314‑3784601
                                                                                                                                                    |                                                                                                                                           
cagcgcagcgGCACCGCTTTCGTGCTGATCAGCCATGACCTGAGCCTGGTGCAGTACTTCTGCCAGCGGGTTGTGCTGCTGCACGGCGGGCGCATCGTGGAGGAACGCCCCGTCACGCACGACCTGTGCTTTGCGCACCCCGTGGGCCgg                                                                                                                                            >  1:1107010/1‑150 (MQ=255)
    gcagcgGCACCGCTTTCGTGCTGATCAGCCATGACCTGAGCCTGGTGCAGTACTTCTGCCAGCGGGTTGTGCTGCTGCACGGCGGGCGCATCGTGGAGGAACGCCCCGTCACGCACGACCTGTGCTTTGCGCACCCCGTGGGCCTGCAGc                                                                                                                                        >  2:171218/1‑150 (MQ=255)
    gcagcgGCACCGCTTTCGTGCTGATCAGCCATGACCTGAGCCTGGTGCAGTACTTCTGCCAGCGGGTTGTGCTGCTGCACGGCGGGCGCATCGTGGAGGAACGCCCCGTCACGCACGACCTGTGCTTTGCGCACCCCGTGGGCCTGCAGc                                                                                                                                        >  2:171297/1‑150 (MQ=255)
        cgGCACCGCTTTCGTGCTGATCAGCCATGACCTGAGCCTGGTGCAGTACTTCTGCCAGCGGGTTGTGCTGCTGCACGGCGGGCGCATCGTGGAGGAACGCCCCGTCACGCACGACCTGTGCTTTGCGCACCCCGTGGGCCGGCAGCTGCa                                                                                                                                    <  1:622729/150‑1 (MQ=255)
              cGCTTTCGTGCTGATCAGCCATGACCTGAGCCTGGTGCAGTACTTCTGCCAGCGGGTTGTGCTGCTGCACGGCGGGCGCATCGTGGAGGAACGCCCCGTCACGCACGACCTGTGCTTTGCGCACCCCGTGGGCCGGCAGCTGCAggcggc                                                                                                                              >  1:643180/1‑150 (MQ=255)
                cTTTCGTGCTGATCAGCCATGACCTGAGCCTGGTGCAGTACTTCTGCCAGCGGGTTGTGCTGCTGCACGGCGGGCGCATCGTGGAGGAACGCCCCGTCACGCACGACCTGTGCTTTGCGCACCCCGTGGGCCTGCAGCTGCCggcccacg                                                                                                                            <  1:171218/150‑6 (MQ=255)
                cTTTCGTGCTGATCAGCCATGACCTGAGCCTGGTGCAGTACTTCTGCCAGCGGGTTGTGCTGCTGCACGGCGGGCGCATCGTGGAGGAACGCCCCGTCACGCACGACCTGTGCTTTGCGCACCCCGTGGGCCTGCAGCTGCCggcccacg                                                                                                                            <  1:171297/150‑6 (MQ=255)
                cTTTCGTGCTGATCAGCCATGACCTGAGCCTGGTGCAGTACTTCTGCCAGCGGGTTGTGCTGCTGCACGGCGGGCGCATCGTGGAGGAACGCCCCGTCACGCACGACCTGTGCTTTGCGCACCCCGTGGGCCGGCAGCTGCAGGcggcgg                                                                                                                            <  2:420984/150‑1 (MQ=255)
                    cGTGCTGATCAGCCATGACCTGAGCCTGGTGCAGTACTTCTGCCAGCGGGTTGTGCTGCTGCACGGCGGGCGCATCGTGGAGGAACGCCCCGTCACGCACGACCTGTGCTTTGCGCACCCCGTGGGCCGGCAGCTGCAGGCGGCGGTGtt                                                                                                                        <  1:500858/150‑1 (MQ=255)
                               gCCATGACCTGAGCCTGGTGCAGTACTTCTGCCAGCGGGTTGTGCTGCTGCACGGCGGGCGCATCGTGGAGGAACGCCCCGTCACGCACGACCTGTGCTTTGCGCACCCCGTGGGCCGGCAGCTGCAGGCGGCGGTGTTGCCTGTGCGcc                                                                                                             >  2:905865/1‑150 (MQ=255)
                                           gCCTGGTGCAGTACTTCTGCCAGCGGGTTGTGCTGCTGCACGGCGGGCGCATCGTGGAGGAACGCCCCGTCACGCACGACCTGTGCTTTGCGCACCCCGTGGGCCGGCAGCTGCAGGCGGCGGTGTTGCCTGTGCGCCCGCAGAGGAGGc                                                                                                 <  2:462815/150‑1 (MQ=255)
                                            ccTGGTGCAGTACTTCTGCCAGCGGGTTGTGCTGCTGCACGGCGGGCGCATCGTGGAGGAACGCCCCGTCACGCACGACCTGTGCTTTGCGCACCCCGTGGGCCGGCAGCTGCAGGCGGCGGTGTTGCCTGTGCGCCCGCAGAGGAGGcc                                                                                                <  2:756054/150‑1 (MQ=255)
                                            ccTGGTGCAGTACTTCTGCCAGCGGGTTGTGCTGCTGCACGGCGGGCGCATCGTGGAGGAACGCCCCGTCACGCACGACCTGTGCTTTGCGCACCCCGTGGGCCGGCAGCTGCAGGCGGCGGTGTTGCCTGTGCGCCCGCAGAGGAGGcc                                                                                                <  2:1107010/150‑1 (MQ=255)
                                                 tGCAGTACTTCTGCCAGCGGGTTGTGCTGCTGCACGGCGGGCGCATCGTGGAGGAACGCCCCGTCACGCACGACCTGTGCTTTGCGCACCCCGTCGGCCGGCAGCTGCAGGCGAGGGTGTTGCCTGCGCGCCCGCAGAGGAGGCCATAAc                                                                                           >  2:368159/1‑150 (MQ=255)
                                                    aGTACTTCTGCCAGCGGGTTGTGCTGCTGCACGGCGGGCGCATCGTGGAGGAACGCCCCGTCACGCACGACCTGTGCTTTGCGCACCCCGTGGGCCGGCAGCTGCAGGCGGCGGTGTTGCCTGTGCGCCCGCAGAGGAGGCCATCACCGc                                                                                        >  1:729148/1‑150 (MQ=255)
                                                     gTACTTCTGCCAGCGGGTTGTGCTGCTGCACGGCGGGCGCATCGTGGAGGAACGCCCCGTCACGCACGACCTGTGCTTTGCGCACCCCGTGGGCCGGCAGCTGCAGGCGGCGGTGTTGCCTGTGCGCCCGCAGAGGAGGCCATCACCGCa                                                                                       >  1:729404/1‑150 (MQ=255)
                                                      tACTTCTGCCAGCGGGTTGTGCTGCTGCACGGCGGGCGCATCGTGGAGGAACGCCCCGTCACGCACGACCTGTGCTTTGCGCACCCCGTGGGCCGGCAGCTGCAGGCGGCGGTGTTGCCTGTGCGCCCGCAGAGGAGGCCATCACCGCaa                                                                                      <  2:729404/150‑1 (MQ=255)
                                                         ttCTGCCAGCGGGTTGTGCTGCTGCACGGCGGGCGCATCGTGGAGGAACGCCCCGTCACGCACGACCTGTGCTTTGCGCACCCCGTGGGCCGGCAGCTGCAGGCGGCGGTGTTGCCTGTGCGCCCGCAGAGGAGGCCATCACCGCAAg                                                                                     >  1:946786/1‑148 (MQ=255)
                                                         ttCTGCCAGCGGGTTGTGCTGCTGCACGGCGGGCGCATCGTGGAGGAACGCCCCGTCACGCACGACCTGTGCTTTGCGCACCCCGTGGGCCGGCAGCTGCAGGCGGCGGTGTTGCCTGTGCGCCCGCAGAGGAGGCCATCACCGCAAg                                                                                     <  2:946786/148‑1 (MQ=255)
                                                                 gCGGGTTGTGCTGCTGCACGGCGGGCGCATCGTGGAGGAACGCCCCGTCGCGCACGACCTGTGCTTTGCGCACCCCGTGGGCCGGCAGCTGCAGGCGGCGGTGTTGCCTGTGCGCCCGCAGAGGAGGCCATCACCGCAAGGCCTGCCAAc                                                                           <  1:358871/150‑1 (MQ=255)
                                                                 gCGGGTTGTGCTGCTGCACGGCGGGCGCATCGTGGAGGAACGCCCCGTCACGCACGACCTGTGCTTTGCGCACCCCGTGGGCCGGCAGCTGCAGGCGGCGGTGTTGCCTGTGCGCCCGCAGAGGAGGCCATCACCGCAAGGCCTGCCAAc                                                                           >  2:525528/1‑150 (MQ=255)
                                                                  cGGGTTGTGCTGCTGCACGGCGGGCGCATCGTGGAGGAACGCCCCGTCACGCACGACCTGTGCTTTGCGCACCCCGTGGGCCGGCAGCTGCAGGCGGCGGTGTTGCCTGTGCCGCGGCAGAGGAGGCCATCACCGCAAGGCCTGCCAACa                                                                          <  1:885999/150‑1 (MQ=255)
                                                                     gTTGTGCTGCTGCACGGCGGGCGCATCGTGGAGGAACGCCCCGTCACGCACGACCTGTGCTTTGCGCACCCCGTGGGCCGGCAGCTGCAGGCGGCGGTGTTGCCTGTGCGCCCGCAGAGGAGGCCATCACCGCAAGGCCTGCCAACAGCt                                                                       <  1:905865/150‑1 (MQ=255)
                                                                          gctgctgcACGGCGGGCGCATCGTGGAGGAACGCCCCGTCACGCACGACCTGTGCTTTGCGCACCCCGTGGGCCGGCAGCTGCAGGCGGCGGTGTTGCCTGTGCGCCCGCAGAGGAGGCCATCACCGCAAGGCCTGCCAACAGCTGcaca                                                                  <  2:643180/150‑1 (MQ=255)
                                                                              ctgcACGGCGGGCGCATCGTGGAGGAACGCCCCGTCACGCACGACCTGTGCTTTGCGCACCCCGTGGGCCGGCAGCTGCAGGCGGCGgt                                                                                                                           <  2:449095/89‑1 (MQ=255)
                                                                              ctgcACGGCGGGCGCATCGTGGAGGAACGCCCCGTCACGCACGACCTGTGCTTTGCGCACCCCGTGGGCCGGCAGCTGCAGGCGGCGgt                                                                                                                           >  1:449095/1‑89 (MQ=255)
                                                                                             aTCGTGGAGGAACGCCCCGTCACGCACGACCTGTGCTTTGCGCACCCCGTGGGCCGGCAGCTGCAGGCGGCGGTGTTGCCTGTGCGCCCGCAGAGGAGGCCATCACCGCAAGGCCTGCCAACAGCTGCACACTGACACCACAGACTGGCt                                               >  2:241495/1‑150 (MQ=255)
                                                                                                 tGGAGGAACGCCCCGTCACGCACGACCTGTGCTTTGCGCACCCCGTGGGCCGGCAGCTGCAGGCGGCGGTGTTGCCTGTGCGCCCGCAGAGGAGGCCATCACCGCAAGGCCTGCCAACAGCTGCACACTGACACCACAGACTGGCTTTCg                                           >  1:357645/1‑150 (MQ=255)
                                                                                                                 cacgcacgACCTGTGCTTTGCGCACCCCGTGGGCCGGCAGCTGCAGGCGGCGGTGTTGCCTGTGCGCCCGCAGAGGAGGCCATCACCGCAAGGCCTGCCAACAGCTGCACACTGACACCACAGACTGGCTTTCGGATCGTCGGAAAACAg                           <  2:729148/150‑1 (MQ=255)
                                                                                                                   cgcacgACCTGTGCTTTGCGCACCCCGTGGGCCGGCAGCTGCAGGCGGCGGTGTTGCCTGTGCGCCCGCAGAGGAGGCCATCACCGCAAGGCCTGCCAACAGCTGCACACTGACACCACAGACTGGCTTTCGGATCGTCGGAAAACAGGc                         >  1:535059/1‑150 (MQ=255)
                                                                                                                   cgcacgACCTGTGCTTTGCGCACCCCGTGGGCCGGCAGCTGCAGGCGGCGGTGTTGCCTGTGCGCCCGCAGAGGAGGCCATCACCGCAAGGCCTGCCAAAAGCTGCACACTGACACCACAGACTGGCTTTCGGATCGTCGGAAAACAGGc                         <  1:525528/150‑1 (MQ=255)
                                                                                                                    gcacgACCTGTGCTTTGCGCACCCCGTGGGCCGGCAGCTGCAGGCGGCGGTGTTGCCTGTGCGCCCGCAGAGGAGGCCATCACCGCAAGGCCTGCCAACAGCTGCACACTGACACCACAGACTGGCTTTCGGATCGTCGGAAAACAGGCt                        >  1:1026536/1‑150 (MQ=255)
                                                                                                                     cacgACCTGTGCTTTGCGCACCCCGTGGGCCGGCAGCTGCAGGCGGCGGTGTTGCCTGTGCGCCCGCAGAGGAGGCCATCACCGCAAGGCCTGCCAACAGCTGCACACTGACACCACAGACTGGCTTTCGGAt                                        <  1:993300/133‑1 (MQ=255)
                                                                                                                     cacgACCTGTGCTTTGCGCACCCCGTGGGCCGGCAGCTGCAGGCGGCGGTGTTGCCTGTGCGCCCGCAGAGGAGGCCATCACCGCAAGGCCTGCCAACAGCTGCACACTGACACCACAGACTGGCTTTCGGAt                                        >  2:993300/1‑133 (MQ=255)
                                                                                                                     cacgACCTGTGCTTTGCGCACCCCGTGGGCCGGCAGCTGCAGGCGGCGGTGTTGCCTGTGCGCCCGCAGAGGAGGCCATCACCGCAAGGCCTGCCAACAGCTGCACACTGACACCACAGACTGGCTTTCGGATCGTCGGAAAACAGGCTa                       <  1:241495/150‑1 (MQ=255)
                                                                                                                          ccTGTGCTTTGCGCACCCCGTGGGCCGGCAGCTGCAGGCGGCGGTATTGCCTCTGCGTCATCAGAGGAGGCAATGACTGCAAGGCCTGCCAACAGCTGCACACTGACACCACAGACTGGCTTTCGGATCGTCGGAAAACAGGCTATGTTc                  >  1:147592/1‑150 (MQ=255)
                                                                                                                           cTGTGCTTTGCGCACCCCGTGGGCCGGCAGCTGCAGGCGGCGGTGTTGCCTGTGCGCCCGCAGAGGAGGCCATCACCGCAAGGCCTGCCAACAGCTGCACACTGACACCACAGACTGGCTTTCGGATCGTCGGAAAACAGGCTATGTTCa                 >  1:119547/1‑150 (MQ=255)
                                                                                                                                cTTTGCGCACCCCGTGGGCCGGCAGCTGCAGGCGGCGGTGTTGCCTGTGCGCCCGCAGAGGAGGCCATCACCGCAAGGCCTGCCAACAGCTGCACACTGACACCACAGACTGGCTTTCGGATCGTCGGAAAACAGGCTATGTTCACCAGa            >  1:6588/1‑150 (MQ=255)
                                                                                                                                    gcgcACCCCGTGGGCCGGCAGCTGCAGGCGGCGGTGTTGCCTGTGCGCCCGCAGAGGAGGCCATCACCGCAAGGCCTGCCAACAGCTGCACACTGacac                                                           <  1:432626/99‑1 (MQ=255)
                                                                                                                                    gcgcACCCCGTGGGCCGGCAGCTGCAGGCGGCGGTGTTGCCTGTGCGCCCGCAGAGGAGGCCATCACCGCAAGGCCTGCCAACAGCTGCACACTGacac                                                           >  2:432626/1‑99 (MQ=255)
                                                                                                                                      gcACCCCGTGGGCCGGCAGCTGCAGGCGGCGGTGTTGCCTGTGCGCCCGCAGAGGAGGCCATCACCGCAAGGCCTGCCAACAGCTGCACACTGACACCACAGACTGGCTTTCGGATCGTCGGAAAACAGGCTATGTTCACCAGACTGATc      >  2:627278/1‑150 (MQ=255)
                                                                                                                                          cccGTGGGCCGGCAGCTGCAGGCGGCGGTGTTGCCTGTGCGCCCGCAGAGGAGGCCATCACCGCAAGGCCTGCCAACAGCTGCACACTGACACCACAGACTGGCTTTCGGAACGTCGGAAAACAGGCTAGGTGCACCAGACGGAACTgcg  >  2:861419/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                           
CAGCGCAGCGGCACCGCTTTCGTGCTGATCAGCCATGACCTGAGCCTGGTGCAGTACTTCTGCCAGCGGGTTGTGCTGCTGCACGGCGGGCGCATCGTGGAGGAACGCCCCGTCACGCACGACCTGTGCTTTGCGCACCCCGTGGGCCGGCAGCTGCAGGCGGCGGTGTTGCCTGTGCGCCCGCAGAGGAGGCCATCACCGCAAGGCCTGCCAACAGCTGCACACTGACACCACAGACTGGCTTTCGGATCGTCGGAAAACAGGCTATGTTCACCAGACTGATCTGCG  >  NC_002947/3784314‑3784601

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑