| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 58,747 | T→A | 21.8% | intergenic (‑658/+30) | PP_5424 ← / ← PP_0050 | hypothetical protein/hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 58,747 | 0 | T | A | 21.8% | 113.3 / 31.2 | 55 | intergenic (‑658/+30) | PP_5424/PP_0050 | hypothetical protein/hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base T (23/20); new base A (5/7); total (28/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.28e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.72e-01 | |||||||||||
GTGAGTAGGGATTTCTCGGCTTTTCTGTGAAAAGCACCCCTTCATTTCACAAAAAAAATGGCGAAGATTCCAATTTCACGGGGTCTGGCCTATCACCTATTTTGTGAACCTACAAGGTGTAATTCGCCAGACACTAAAAAGCCCGCTGCTGCGGGCTTTTTAGTGCCTGCAAACAATCAGTAGCCGGTGAGAATGTTCACGATCACACCGCTGGCAATGGCCACCAGAACATAGTCGCCATTCACATACAGCCAACGATGATCACGAGGTGGCGCATACAGGTGGCGCGCTTTCCA > NC_002947/58601‑58896 | gTGAGTAGGGATTTCTCGGCTTTTCTGTGAAAAGCACCCCTTCATTTCACAAAAAAAATGGCGAAGATTCCAATTTCACGGGGTCTGGCCTATCACCTATTTTGTGAACCTACAAGGTGTAATTCGCCAGACACTAAAAAGCCCgcagca > 1:27951/1‑149 (MQ=255) gggATTTCTCGGCTTTTCTGTGAAAAGCACCCCTTCATTTCACAAAAAAAATGGCGAAGATTCCAATTTCACGGGGTCTGGCCTATCACCTATTTTGTGAACCTACAAGGTGTAATTCGCCAGACACTAAAAAGCCCgctgc > 1:344540/1‑142 (MQ=255) gggATTTCTCGGCTTTTCTGTGAAAAGCACCCCTTCATTTCACAAAAAAAATGGCGAAGATTCCAATTTCACGGGGTCTGGCCTATCACCTATTTTGTGAACCTACAAGGTGTAATTCGCCAGACACTAAAAAGCCCgctgc < 2:344540/142‑1 (MQ=255) gATTTCTCGGCTTTTCTGTGAAAAGCACCCCTTCATTTCACAAAAAAAATGGCGAAGATTCCAATTTCACGGGGTCTGGCCTATCACCTATTTTGTGAACCTACAAGGTGTAATTCGCCAGACACTAAAAAGCCCGCAGCAGCGGGCttt < 2:27951/150‑1 (MQ=255) gATTTCTCGGCTTTTCTGTGAAAAGCACCCCTTCATTTCACAAAAAAAATGGCGAAGATTCCAATTTCACGGGGTCTGGCCTATCACCTATTTTGTGAACCTACAAGGTGTAATTCGCCAGACACTAAAAAGCCCGCAGCAGCGGGCttt < 1:577165/150‑1 (MQ=255) ttCTCGGCTTTTCTGTGAAAAGCACCCCTTCAACTCAGAAAAAAAATGGCGAAGATTCCAATTTCACGGGGTCTGGCCTATCACCTATTTTGTGAACCTACAAGGTGTAATTCGCCAGGCACTAAAAAGCCCGCAGCAGCGGGCTTTTTa < 2:1188019/150‑1 (MQ=255) ttttCTGTGAAAAGCACCCCTTCATTTCACAAAAAAAATGGCGAAGATTCCAATTTCACGGGGTCTGGCCTATCACCTATTTTGTGAACCTACAAGGTGTAATTCGCCAGACACTAAAAAGCCCGCTGCTGCGGGCTTTTTAGTGCCTGc > 1:304491/1‑150 (MQ=255) gtgtgAAAAGCACCCCTTCATTTCACAAAAAAAATGGCGAAGATTCCAATTTCACGGGGTCTGGCCTATCACCTATTTTGTGAACCTACAAGGTGTAATTCGCCAGACACTAAAAAGCCCGCAGCAGCGGGCTTTTTAGTGTCTggcgaa < 1:1145401/149‑6 (MQ=38) tCATTTCACAAAAAAAATGGCGAAGATTCCAATTTCACGGGGTCTGGCCTATCACCTATTTTGTGAACCTACAAGGTGTAATTCGCCAGACACTAAAAAGCCCGCTGCTGCGGGCTTTTTAGTGCCTGCAAACAATCAGTAGCCGGTgag < 1:413760/150‑1 (MQ=255) acaAAAAAAATGGCGAAGATTCCAATTTCACGGGGTCTGGCCTATCACCTATTTTGTGAACCTACAAGGTGTAATTCGCCAGACACTAAAAAGCCCGCTGCTGCGGGCTTTTTAGTGCCTGCAAACaa > 2:25026/1‑128 (MQ=255) acaAAAAAAATGGCGAAGATTCCAATTTCACGGGGTCTGGCCTATCACCTATTTTGTGAACCTACAAGGTGTAATTCGCCAGACACTAAAAAGCCCGCTGCTGCGGGCTTTTTAGTGCCTGCAAACaa < 1:25026/128‑1 (MQ=255) aaaaaaaaTGGCGAAGATTCCAATTTCACGGGGTCTGGCCTATCACCTATTTTGTGAACCTACAAGGTGTAATTCGCCAGACACTAAAAAGCCCGCTGCTGCGGGCTTTTTAGTGCCTGCAAACAATCAGTAGCCGGTGAGAATGTTCAc > 2:380985/1‑150 (MQ=255) aaaaaaaTGGCGAAGATTCCAATTTCACGGGGTCTGGCCTATCACCTATTTTGTGAACCTACAAGGTGTAATTCGCCAGACACTAAAAAGCCCGCTGCTGCGGGCTTTTTAGTGCCTGCAAACAATCAGTAGCCGGTg < 1:1013512/138‑1 (MQ=255) aaaaaaaTGGCGAAGATTCCAATTTCACGGGGTCTGGCCTATCACCTATTTTGTGAACCTACAAGGTGTAATTCGCCAGACACTAAAAAGCCCGCTGCTGCGGGCTTTTTAGTGCCTGCAAACAATCAGTAGCCGGTg > 2:1013512/1‑138 (MQ=255) aaaaaaaTGGCGAAGATTCCAATTTCACGGGGTCTGGCCTATCACCTATTTTGTGAACCTACAAGGTGTAATTCGCCAGACACTAAAAAGCCCGCTGCTGCGGGCTTTTTAGTGCCTGCAAACAATCAGTAGCCGGTGAGAATGTTCACg > 1:923495/1‑150 (MQ=255) aaaaaaTGGCGAAGATTCCAATTTCACGGGGTCTGGCCTATCACCTATTTTGTGAACCTACAAGGTGTAATTCGCCAGACACTAAAAAGCCCGCTGCTGCGGGCTTTTTAGTGCCTGCAAACAATCAGTAGCCGGTGAGAATGTTCACGa > 1:724332/1‑150 (MQ=255) aaaaaTGGCGAAGATTCCAATTTCACGGGGTCTGGCCTATCACCTATTTTGTGAACCTACAAGGTGTAATTCGCCAGACACTAAAAAGCCCGCTGCTGCGGGCTTTTTAGTGCCTGCAAACAATCAGTAGCCGGTGAGAATGTTCACGAt < 2:832987/150‑1 (MQ=255) gAAGATTCCAATTTCACGGGGTCTGGCCTATCACCTATTTTGTGAACCTACAAGGTGTAATTCGCCAGACACTAAAAAGCCCGCTGCTGCGGGCTTTTTAGTGCCTGCAAACAATCAGTAGCCGGTGAGAATGTTCACGATCACAcc < 2:608130/147‑1 (MQ=255) gAAGATTCCAATTTCACGGGGTCTGGCCTATCACCTATTTTGTGAACCTACAAGGTGTAATTCGCCAGACACTAAAAAGCCCGCTGCTGCGGGCTTTTTAGTGCCTGCAAACAATCAGTAGCCGGTGAGAATGTTCACGATCACAcc > 1:608130/1‑147 (MQ=255) gAAGATTCCAATTTCACGGGGTCTGGCCTATCACCTATTTTGTGAACCTACAAGGTGTAATTCGCCAGACACTAAAAAGCCCGCTGCTGCGGGCTTTTTAGTGCCTGCAAACAATCAGTAGCCGGTGAGAATGTTCACGATCACACCGCt < 2:304491/150‑1 (MQ=255) aaGATTCCAATTTCACGGGGTCTGGCCTATCACCTATTTTGTGAACCTACAAGGTGTAATTCGCCAGACACTAAAAAGCCCGCTGCTGCGGGCTTTTTAGTGCCTGCAAACAATCAGTAGCCGGTGAGAATGTTCACGATCACACCGCTg > 1:1090416/1‑150 (MQ=255) tCCAATTTCACGGGGTCTGGCCTATCACCTATTTTGTGAACCTACAAGGTGTAATTCGCCAGACACTAAAAAGCCCGCTGCTGCGGGCtttt < 2:655830/92‑1 (MQ=255) tCCAATTTCACGGGGTCTGGCCTATCACCTATTTTGTGAACCTACAAGGTGTAATTCGCCAGACACTAAAAAGCCCGCTGCTGCGGGCtttt > 1:655830/1‑92 (MQ=255) tCCAATTTCACGGGGTCTGGCCTATCACCTATTTTGTGAACCTACAAGGTGTAATTCGCCAGACACTAAAAAGCCCGCTGCTGCGGGCTTTTTAGTGCCTGCAAACAATCAGTAGCCGGTGAGAATGTTCACGATCACACCGCTGGCAAt > 2:34266/1‑150 (MQ=255) ccAATTTCACGGGGTCTGGCCTATCACCTATTTTGTGAACCTACAAGGTGTAATTCGCCAGACACTAAAAAGCCCGCTGCTGCGGGCTTTTTAGTGCCTGCAAACAATCAGTAGCCGGTGAGAATGTTCACGATCACACCGCTGGCAATg < 2:1090416/150‑1 (MQ=255) cAATTTCACGGGGTCTGGCCTATCACCTATTTTGTGAACCTACAAGGTGTAATTCGCCAGACACTAAAAAGCCCGCTGCTGCGGGCTTTTTAGTGCCTGCAAACAATCAGTAGCCGGTGAGAATGTTCACGATca > 2:825872/1‑135 (MQ=255) cAATTTCACGGGGTCTGGCCTATCACCTATTTTGTGAACCTACAAGGTGTAATTCGCCAGACACTAAAAAGCCCGCTGCTGCGGGCTTTTTAGTGCCTGCAAACAATCAGTAGCCGGTGAGAATGTTCACGATca < 1:825872/135‑1 (MQ=255) aaTTTCACGGGGTCTGGCCTATCACCTATTTTGTGAACCTACAAGGTGTAATTCGCCAGACACTAAAAAGCCCGCTGCTACGGGCTTTTTAGTGCCTGCAAACAATCAGTAGCCGGTGAGAATGTTCACGATCACACCGCTGGCAATGGc > 1:404737/1‑150 (MQ=255) cGGGGTCTGGCCTATCACCTATTTTGTGAACCTACAAGGTGTAATTCGCCAGACACTAAAAAGCCCGCTGCTGCGGGCTTTTTAGTGCCTGCaaa < 1:110070/95‑1 (MQ=255) cGGGGTCTGGCCTATCACCTATTTTGTGAACCTACAAGGTGTAATTCGCCAGACACTAAAAAGCCCGCTGCTGCGGGCTTTTTAGTGCCTGCaaa > 2:110070/1‑95 (MQ=255) cTGGCCTATCACCTATTTTGTGAACCTACAAGGTGTAATTCGCCAGACACTAAAAAGCCCGCTGCTGCGGGCTTTTTAGTGCCTGCAAACAATCAGTAGCCGGTGAGAATGTTCACGATCACACCGCTGGCAATGGCCACCAGAACATAg < 2:773089/150‑1 (MQ=255) ggCCTATCACCTATTTTGTGAACCTACAAGGTGTAATTCGCCAGACACTAAAAAGCCCGCTGCTGCGGGCTTTTTAGTGCCTGCAAACAATCAGTAGCCGGTGAGAATGTTCACGATCACACCGCTGGCAATGGCCACCAGAACATAGTc < 2:1053476/150‑1 (MQ=255) ggCCTATCACCTATTTTGTGAACCTACAAGGTGTAATTCGCCAGACACTAAAAAGCCCGCTGCTGCGGGCTTTTTAGTGCCTGCAAACAATCAGTAGCCGGTGAGAATGTTCACGATCACACCGCTGGCAATGGCCACCAGAACATAGTc < 2:729774/150‑1 (MQ=255) gCCTATCACCTATTTTGTGAACCTACAAGGTGTAATTCGCCAGACACTAAAAAGCCCGCTGCTGCGGGCTTTTTAGTGCCTGCAAACAATCAGTAGCCGGTGAGAATGTTCACGAt < 2:559866/116‑1 (MQ=255) gCCTATCACCTATTTTGTGAACCTACAAGGTGTAATTCGCCAGACACTAAAAAGCCCGCTGCTGCGGGCTTTTTAGTGCCTGCAAACAATCAGTAGCCGGTGAGAATGTTCACGAt > 1:559866/1‑116 (MQ=255) aCCTATTTTGTGAACCTACAAGGTGTAATTCGCCAGACACTAAAAAGCCCGCTGCTGCGGGCTTTTTAGTGCCTGCAAACAATCAGTAGCCGGTGAGAATGTTCACGATCACACCGCTGGCAATGGCCACCAGAACATAGTCGCCATTca < 2:18375/150‑1 (MQ=255) cTATTTTGTGAACCTACAAGGTGTAATTCGCCACATTCTAAAAAGCCCGCTGCTGCGGGCTTTTTAGTGCCTGCAAACAATCAGTAGCCGGTGAGAATGTTCACGATCACACCGCTGGCAATGGCCACCAGAACATAGTCGCCATTcaca > 1:285952/1‑150 (MQ=255) tATTTTGTGAACCTACAAGGTGTAATTCGCCAGACACTAAAAAGCCCGCTGCTGCGGGCTTTTTAGTGCCTGCAAACAATCAGTAGCCGGTGAGAATGTTCACGATCACACCGCTGGCAATGGCCACCAGGACATAGTCGCCATTCACAt > 1:77990/1‑150 (MQ=255) aTTTTGTGAACCTACAAGGTGTAATTCGCCAGACACTAAAAAGCCCGCTGCTGCGGGCTTTTTAGTGCCTGCAAACAATCAGTAGCCGGTGAGAATGTTCACGATCACACCGCTGGCAATGGCCACCAGAACATAGTCGCCATTCACATa > 1:231921/1‑150 (MQ=255) tttGTGAACCTACAAGGTGTAATTCGCCAGCCCCTATGAATTCCGCTGCTGCGGGCTTTTTAGTGCCTGCAAACAATCAGTAGCCGGTGAGAATGTTCACGATCACACCGCTGGCAATGGCCACCAGAACATAGTCGCCATTCACATACa < 2:285952/150‑1 (MQ=255) aCAAGGTGTAATTCGCCAGACACTAAAAAGCCCGCTGCTGCGGGCTTTTTAGTGCCTGCAAACAATCAGTAGCCGGTGAGAATGTTCACGATCACACCGCTGGCAATGGCCATCAGAACATAGTCGCCATTCACATACAGCCAACgatga < 2:923495/150‑1 (MQ=255) aGGTGTAATTCGCCAGACACTAAAAAGCCCGCTGCTGCGGGCTTTTTAGTGCCTGCAAACAATCAGTAGCCGGTGAGAATGTTCACGATCACACCGCTGGCAATGGCCACCAGAACa > 2:1136267/1‑117 (MQ=255) aGGTGTAATTCGCCAGACACTAAAAAGCCCGCTGCTGCGGGCTTTTTAGTGCCTGCAAACAATCAGTAGCCGGTGAGAATGTTCACGATCACACCGCTGGCAATGGCCACCAGAACa < 1:1136267/117‑1 (MQ=255) tAATTCGCCAGACACTAAAAAGCCCGCTGCTGCGGGCTTTTTAGTGCCTGCAAACAATCAGTAGCCGGTGAGAATGTTCACGATCACACCGCTGGCAATGGCCACCAGAACATAGTCGCCATTCACATACAGCCAACGATGATCACGAgg > 1:476756/1‑150 (MQ=255) ttCGCCAGACACTAAAAAGCCCGCTGCTGCGGGCTTTTTAGTGCCTGCAAACAATCAGTAGCCGGTGAGAATGTTCACGATCACACCGCTGGCAATGGCCACCAGAACATAGTCGCCATTCACATACAGCCAACGATGATCACGAGGTgg > 2:946886/1‑150 (MQ=255) ttCGCCAGACACTAAAAAGCCCGCTGCTGCGGGCTTTTTAGTGCCTGCAAACAATCAGTAGCCGGTGAGAATGTTCACGATCACACCGCTGGCAATGGCCACCAGAACATAGTCGCCATTCACATACAGCCAACGATGATCACGAGGTgg < 1:946886/150‑1 (MQ=255) gCCAGACACTAAAAAGCCCGCTGCTGCGGGCTTTTTAGTGCCTGCAAACAATCAGTAGCCGGTGAGAAt < 2:212513/69‑1 (MQ=255) gCCAGACACTAAAAAGCCCGCTGCTGCGGGCTTTTTAGTGCCTGCAAACAATCAGTAGCCGGTGAGAAt > 1:212513/1‑69 (MQ=255) tAAAAAGCCCGCAGCAGCGGGCTTTTTAGTGTCTGCAAACAATCAGTAGCCGGTGAGAATGTTCACGATCACACCGCTGGCAATGGCCACCAGAACATAGTCGc < 1:692578/104‑1 (MQ=38) tAAAAAGCCCGCAGCAGCGGGCTTTTTAGTGTCTGCAAACAATCAGTAGCCGGTGAGAATGTTCACGATCACACCGCTGGCAATGGCCACCAGAACATAGTCGc > 2:692578/1‑104 (MQ=38) tAAAAAGCCCGCAGCAGCGGGCTTTTTAGTGTCTGCAAACAATCAGTAGCCGGTGAGAATGTTCACGATCACACCGCTGGCAATGGCCACCAGAACATAGTCGCCATTCACATACAGCCAACGATGATCGCGAGGt < 1:229638/136‑1 (MQ=39) tAAAAAGCCCGCAGCAGCGGGCTTTTTAGTGTCTGCAAACAATCAGTAGCCGGTGAGAATGTTCACGATCACACCGCTGGCAATGGCCACCAGAACATAGTCGCCATTCACATACAGCCAACGATGATCGCGAGGt > 2:229638/1‑136 (MQ=39) tAAAAAGCCCGCAGCAGCGGGCTTTTTAGTGCCTGCAAACAATCAGTAGCCGGTGAGAATGTTCACGATCACACCGCTGGCAATGGCCACCAGAACATAGTCGCCATTCACATACAGCCAACGATGATCACGAGGTGGCGCATACAGGTg > 2:772247/1‑150 (MQ=255) aaaaaGCCCGCAGCAGCGGGCTTTTTAGTGTCTGCAAACAATCAGTAGCCGGTGAGAATGTTCACGATCACACTGCTGGCAATGGCCAACAGAACAGAGTCGCCATTCACATACAGCCAACGATGATCACGAGGTGGCGCATACAGGTgg > 1:271707/1‑150 (MQ=255) aaaaGCCCGCTGCTGCGGGCTTTTTAGTGCCTGCAAACAATCAGTAGCCGGTGAGAATGTTCACGATCACACCGCTGGCAATGGCCGCCAGAACCTTACCGGTCTTCTCATACAGACAACTATGATCACGAGGTGGCGCATACAGGTGgc > 2:634736/1‑150 (MQ=255) aaGCCCGCTGCTGCGGGCTTTTTAGTGCCTGCAAACAATCAGTAGCCGGTGAGAATGTTCACGATCACACCGCTGGCAATGGCCACCAGAACATAGTCGCCATTCACATACAGCCAACGATGATCACGAGGTGGCGCCAACACGGGgcgc > 2:79327/1‑150 (MQ=255) aaGCCCGCAGCAGCGGGCTTTTTAGTGTCTGCAAACAATCAGTAGCCGGTGAGAATGTTCACGATCACACCGCTGGCAATGGCCACCAGAACATAGTc > 1:1114775/1‑98 (MQ=38) aaGCCCGCAGCAGCGGGCTTTTTAGTGTCTGCAAACAATCAGTAGCCGGTGAGAATGTTCACGATCACACCGCTGGCAATGGCCACCAGAACATAGTc < 2:1114775/98‑1 (MQ=38) gctgctGCGGGCTTTTTAGTGCCTGCAAACAATCAGTAGCCGGTGAGAATGTTCACGATCACACCGCTGGCAATGGCCACCAGAACATAGTCGCCATTCACATACAGCCAACGATGATCACGAGGTGGCGCATACAGGTGGCGCGCTTTc > 1:203448/1‑150 (MQ=255) tgctgcGGGCTTTTTAGTGCCTGCAAACAATCAGTAGCCGGTGAGAATGTTCACGATCACACCGCTGGCAATGGCCACCAGAACATAGTCGCCATTCACATACAGCCAACGATGATCACGAGGTGGCGCATACAGGTGGCGCGCTTTCca < 2:724332/150‑1 (MQ=255) | GTGAGTAGGGATTTCTCGGCTTTTCTGTGAAAAGCACCCCTTCATTTCACAAAAAAAATGGCGAAGATTCCAATTTCACGGGGTCTGGCCTATCACCTATTTTGTGAACCTACAAGGTGTAATTCGCCAGACACTAAAAAGCCCGCTGCTGCGGGCTTTTTAGTGCCTGCAAACAATCAGTAGCCGGTGAGAATGTTCACGATCACACCGCTGGCAATGGCCACCAGAACATAGTCGCCATTCACATACAGCCAACGATGATCACGAGGTGGCGCATACAGGTGGCGCGCTTTCCA > NC_002947/58601‑58896 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |