| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 3,869,903 | C→A | 17.0% | E354* (GAG→TAG) ‡ | PP_3419 ← | sigma‑54 dependent transcriptional regulator/response regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 3,869,903 | 0 | C | A | 17.0% | 132.3 / 16.2 | 53 | E354* (GAG→TAG) ‡ | PP_3419 | sigma‑54 dependent transcriptional regulator/response regulator |
| Reads supporting (aligned to +/- strand): ref base C (22/22); new base A (4/5); total (26/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.28e-01 | |||||||||||
CGGCTCGTCAACCAAGGTCATGCACAGGTTCAACTGATGCGCTTGCACCACCTCGTTCGGCGCCAGCAACACCGTCTGCTCCAGCATGTTGCGCAATTCACGCACATTGCCAGGCCAGCTATAACCGAGCAGCAGGC‑TTTCGGCCTCGGCCGAAAAGCGCAGGTTCGGCTTGCCATAGCGCCGGCTATGGTGGGCAAGGAAATGCCGCGCCAGTAGCAGGATGTCCTGCCCACGCGAATACAGGCGCGGCACCTTCAGGGCGATAATGCGAAGGCGGAAGAACAGGTCACGCCTG > NC_002947/3869757‑3870051 | cGGCTCGTCAACCAAGGTCATGCACAGGTTCAACTGATGCGCTTGCACCACCTCGTTCGGCGCCAGCAACACCGTCTGCTCCAGCATGTTGCGCAATTCACGCACATTGCCAGGCCAGCTATAACCGAGCAGCAGGC‑TTtcggcctcggc < 1:1109126/150‑1 (MQ=255) tCGTCAACCAAGGTCATGCACAGGTTCAACTGATGCGCTTGCACCACCTCGTTCGGCGCCAGCAACACCGTCTGCTCCAGCATGTTGCGCAATTCACGCACATTGCCAGGCCAGCTATAACCGAGCAGCAGGC‑TTTCGGCCTCGGCCGaa > 2:161734/1‑150 (MQ=255) gTCAACCAAGGTCATGCACAGGTTCAACTGATGCGCTTGCACCACCTCGTTCGGCGCCAGCAACACCGTCTGCTCCAGCATGTTGCGCAATTCACGCACATTGCCAGGCCAGCTATAACCGAGCAGCAGGCTTTTCGGCCGAGGCCGaaa > 1:1144424/1‑150 (MQ=255) gTCAACCAAGGTCATGCACAGGTTCAACTGATGCGCTTGCACCACCTCGTTCGGCGCCAGCAACACCGTCTGCTCCAGCATGTTGCGCAATTCACGCACATTGCCAGGCCAGCTATAACCGAGCAGCAGGC‑TTTCGGCCTCGGCCGaaaa > 1:1131676/1‑150 (MQ=255) tCAACCAAGGTCATGCACAGGTTCAACTGATGCGCTTGCACCACCTCGTTCGGCGCCAGCAACACCGTCTGCTCCAGCATGTTGCGCAATTCACGCACATTGCCAGGCCAGCTATAACCGAGCAGCAGGC‑TTTCGGCCGAGGCCGaaa < 1:1067626/148‑1 (MQ=255) tCAACCAAGGTCATGCACAGGTTCAACTGATGCGCTTGCACCACCTCGTTCGGCGCCAGCAACACCGTCTGCTCCAGCATGTTGCGCAATTCACGCACATTGCCAGGCCAGCTATAACCGAGCAGCAGGC‑TTTCGGCCGAGGCCGaaa > 2:1067626/1‑148 (MQ=255) cAACCAAGGTCATGCACAGGTTCAACTGATGCGCTTGCACCACCTCGTTCGGCGCCAGCAACACCGTCTGCTCCAGCATGTTGCGCAATTCACGCACATTGCCAGGCCAGCTATAACCGAGCAGCAGGC‑TTTCGGCCTCGGCCGAAAAgc < 2:1090925/150‑1 (MQ=255) aaCCAAGGTCATGCACAGGTTCAACTGATGCGCTTGCACCACCTCGTTCGGCGCCAGCAACACCGTCTGCTCCAGCATGTTGCGCAATTCACGCACATTGCCAGGCCAGCTATAACCGAGCAGCAGGC‑TTTCGGCCTCGGCCGAAAAgcg > 2:982598/1‑150 (MQ=255) cAAGGTCATGCACAGATTCAACTGATGCGCTTGCACCACCTCGTTCGGCGCCAGCAACACCGTCTGCTCCAGCATGTTGCGCAATTCACGCACATTGCCAGGCCAGCTATAACCGAGCAGCAGGCTTTTCGGCCGAGGCCGaaagcctgc < 2:838073/150‑7 (MQ=255) aaGGTCATGCACAGGTTCAACTGATGCGCTTGCACCACCTCGTTCGGCGCCAGCAACACCGTCTGCTCCAGCATGTTGCGCAATTCACGCACATTGCCAGGCCAGCTATAACCGAGCAGCAGGCTTTTCGGCCGAGGCCGaaagcctgct < 2:1144424/150‑8 (MQ=255) gTTCAACTGATGCGCTTGCCCCACCTCGTTCGGCGCCAGCAACACCGTCTGCTCCAGCATGTTGCGCAATTCACGCACATTGCCAGGCCAGCTATAACCGAGCAGCAGGC‑TTTCGGCCTCGGCCGAAAAGCGCAGGTTCGGCTTGCCATa < 2:793389/150‑1 (MQ=255) gTTCAACTGATGCGCTTGCACCACCTCGTTCGGCGCCAGCAACACCGTCTGCTCCAGCATGTTGCGCAATTCACGCACATTGCCAGGCCAGCTATAACCGAGCAGCAGGC‑TTTCGGCCTCGGCCGAAAAGCGCAGGTTCGGCTTGCCATa > 1:249623/1‑150 (MQ=255) gTTCAACTGATGCGCTTGCACCACCACAAGCTGCGCCAGCAACACCGTCTGCTCCAGCATGTTGCGCAATTCACGCACATTGCCAGGCCAGCTATAACCGAGCAGCAGGC‑TTTCGGCCTCGGCCGAAAAGCGCAGGTTCGGCTTGCCATa > 1:31509/1‑150 (MQ=255) ttCAACTGATGCGCTTGCACCACCTCGTTCGGCGCCAGCAACACCGTCTGCTCCAGCATGTTGCGCAATTCACGCACATTGCCAGGCCAGCTATAACCGAGCAGCAGGC‑TTTCGGCCTCGGCCGAAAAGCGCAGGTTCGGCTTGCCATAg > 1:113138/1‑150 (MQ=255) tCAACTGATGCGCTTGCACCACCTCGTTCGGCGCCAGCAACACCGTCTGCTCCAGCATGTTGCGCAATTCACGCACATTGCCAGGCCAGCTATAACCGAGCAGCAGGC‑TTTCGGCCTCGGCCGAAAAGCGCAGGTTCGGCTTGCCATAgc < 1:982598/150‑1 (MQ=255) gcgcTTGCACCACCTCGTTCGGCGCCAGCAACACCGTCTGCTCCAGCATGTTGCGCAATTCACGCACATTGCCAGGCCAGCTATAACCGAGCAGCAGGC‑TTTCGGCCTCGGCCGAAAAGCGCAGGTTCGGCTTGCCATAGCGCCGGCTAt < 2:241787/150‑1 (MQ=255) gcgcTTGCACCACCTCGTTCGGCGCCAGCAACACCGTCTGCTCCAGCATGTTGCGCAATTCACGCACATTGCCAGGCCAGCTATAACCGAGCAGCAGGC‑TTTCGGCCTCGGCCGAAAAGCGCAGGTTCGGCTTGCCATAGCGCCGGCTAt > 2:852742/1‑150 (MQ=255) ccTCGTTCGGCGCCAGCAACACCGTCTGCTCCAGCATGTTGCGCAATTCACGCACATTGCCAGGCCAGCTATAACCGAGCAGCAGGC‑TTTCGGCCTCGGCCGAAAAGCGCAGGTTCGGCTTGCCATAGCGCCGGCTATGGTGGGCAAGGa > 1:829032/1‑150 (MQ=255) tCGTTCGGCGCCAGCAACACCGTCTGCTCCAGCATGTTGCGCAATTCACGCACATTGCCAGGCCAGCTATAACCGAGCAGCAGGC‑TTTCGGCCTCGGCCGAAAAGCGCAGGTTCGGCTTGCCATAGCGCCGGCTATGGTGGGCAAGGaaa < 1:852742/150‑1 (MQ=255) ttCGGCGCCAGCAACACCGTCTGCGCCAGCATGTTGCACAATTCACGCACATTGCCAGGCCAGCTATAACCGAGCAGCAGGC‑TTTCGGCCTCGGCCGAAAAGCGCAGGTTCGGCTTGCCATAGCGCCGGCTATGGTGGGCaa > 2:322450/1‑142 (MQ=255) tgcGGCGCCAGCAACACCGTCTGCTCCAGCATGTTGCACAATTCACGCACATTGCCAGGCCAGCTATAACCGAGCAGCAGGC‑TTTCGGCCTCGGCCGAAAAGCGCAGGTTCGGCTTGCCATAGCGCCGGCTATGGTGGGCaa < 1:322450/140‑1 (MQ=255) aGCAACACCGTCTGCTCCAGCATGTTGCGCAATTCACGCACATTGCCAGGCCAGCTATAACCGAGCAGCAGGC‑TTTCGGCCTCGGCCGAAAAGCGCAGGTTCGGCTTGCCATAGCGCCGGCTATGGTGGGCAAGGAAATGCCGCGCCAGt > 2:611714/1‑150 (MQ=255) acacCGTCTGCTCCAGCATGTTGCGCAATTCACGCACATTGCCAGGCCAGCTATAACCGAGCAGCAGGC‑TTTCGGCCTCGGCCGAAAAGCGCAGGTTCGGCTTGCCATAGCGCCGGCTATGGTGGGCAAGGAAATGCCGCGCCAGTAGCa < 1:386546/150‑1 (MQ=255) acacCGTCTGCTCCAGCATGTTGCGCAATTCACGCACATTGCCAGGCCAGCTATAACCGAGCAGCAGGC‑TTTCGGCCTCGGCCGAAAAGCGCAGGTTCGGCTTGCCATAGCGCCGGCTATGGTGGGCAAGGAAATGCCGCGCCAGTAGCa > 2:1031453/1‑150 (MQ=255) cGTCTGCTCCAGCATGTTGCGCAATTCACGCACATTGCCAGGCCAGCTATAACCGAGCAGCAGGC‑TTTCGGCCTCGGCCGAAAAGCGCAGGTTCGGCTTGCCATAGCGCCGGCTATGGTGGGCAAGGAAATGCCGCGCCAGTAGCAGGAt < 1:1031453/150‑1 (MQ=255) cGTCTGCTCCAGCATGTTGCGCAATTCACGCACATTGCCAGGCCAGCTATAACCGAGCAGCAGGC‑TTTCGGCCTCGGCCGAAAAGCGCAGGTTCGGCTTGCCATAGCGCCGGCTATGGTGGGCAAGGAAATGCCGCGCCAGTAGCAGGAt < 2:23159/150‑1 (MQ=255) cGTCTGCTCCAGCATGTTGCGCAATTCACGCACATTGCCAGGCCAGCTATAACCGAGCAGCAGGC‑TTTCGGCCTCGGCCGAAAAGCGCAGGTTCGGCTTGCCATAGCGCCGGCTATGGTGGGCAAGGAAATGCCGCGCCAGTAGCAGGAt < 2:829032/150‑1 (MQ=255) gcAATTCACGCACATTGCCAGGCCAGCTATAACCGAGCAGCAGGC‑TTTCGGCCTCGGCCGAAAAGCGCAGGTTCGGCTTGCCATAGCGCCGGCTATGGTGGGCAAGGAAATGCCGCGCCAGTAGCAGGATGTCCTGCCCACGCGAATACa > 1:948783/1‑150 (MQ=255) cACGCACATTGCCAGGCCAGCTATAACCGAGCAGCAGGC‑TTTCGGCCTCGGCCGAAAAGCGCAGGTTCGGCTTGCCATAGCGCCGGCTATGGTGGGCAAGGAAATGCCGCGCCAGTAGCAGGATGTCCTGCCCACGCGAATACAGGcgcg > 2:179293/1‑150 (MQ=255) cGCACATTGCCAGGCCAGCTATAACCGAGCAGCAGGC‑TTTCGGCCTAGTACAAAAAGCTCAGGTTCGGCTTGCCATAGCGCCGGCTATGGTGGGCAAGGAAATGCCGCGCCAGTAGCAGGATGTCCTGCCCACGCGAATACAGGCGCGGc < 2:31509/150‑1 (MQ=255) aTTGCCAGGCCAGCTATAACCGAGCAGCAGGC‑TTTCGGCCTCGGCCGAAAAGCGCAGGTTCGGCTTGCCATAGCGCCGGCTATGGTGGGCAAGGAAATGCCGCGCCAGTAGCAGGATGTCCTGCCCACGCGAATACAGGCGCGGCACCtt > 2:786585/1‑150 (MQ=255) ccagCTATAACCGAGCAGCAGGC‑TTTCGGCCTCGGCCGAAAAGCGCAGGTTCGGCTTGCCATAGCGCCGGCTATGGTGGGCAAGGAAATGCCGCGCCAGTAGCAg < 2:665151/105‑1 (MQ=255) ccagCTATAACCGAGCAGCAGGC‑TTTCGGCCTCGGCCGAAAAGCGCAGGTTCGGCTTGCCATAGCGCCGGCTAAGGTGGGCAAGGAAATGCCGCGCCAGTAGCAg > 1:665151/1‑105 (MQ=255) cagCTATAACCGAGCAGCAGGC‑TTTCGGCCTCGGCCGAAAAGCGCAGGTTCGGCTTGCCATAGCGCCGGCTATGGTGGGCAAGGAAATGCcgc > 1:1147688/1‑93 (MQ=255) cagCTATAACCGAGCAGCAGGC‑TTTCGGCCTCGGCCGAAAAGCGCAGGTTCGGCTTGCCATAGCGCCGGCTATGGTGGGCAAGGAAATGCcgc < 2:1147688/93‑1 (MQ=255) agCTATAACCGAGCAGCAGGC‑TTTCGGCCTCGGCCGAAAAGCGCAGGTTCGGCTTGCCATAGCGCCGGCTATGGTGGGCAAGGAAATGCCGCGCCAGTAGCAGGATGTCCTGCCCACGCGAATACAGGCGCGGCACCTTCAGGGCGATaa > 2:835585/1‑150 (MQ=255) agCTATAACCGAGCAGCAGGC‑TTTCGGCCTCGGCCGAAAAGCGCAGGTTCGGCTTGCCATAGCGCCGGCTATGGTGGGCAAGGAAATGCCGCGCCAGTAGCAGGATGTCCTGCCCACGCGAATACAGGCGCGGCACCTTCAGGGCGATaa < 1:302151/150‑1 (MQ=255) agCTATAACCGAGCAGCAGGC‑TTTCGGCCTCGGCCGAAAAGCGCAGGTTCGGCTTGCAATAGCGCCGGCTATGGTGGGCAAGGAAATGCCGCGCCAGTAGCAGGATGTCCTGCCCACGCGAATACAGGCGCGGCACCTTCAGGGCGATaa > 2:767704/1‑150 (MQ=255) gCTATAACCGAGCAGCAGGC‑TTTCGGCCTCGGCCGAAAAGCGCAGGTTCGGCTTGCCATAGCGCCGGCTATGGTGGGCAAGGAAATGCCGCGCCAGTAGCAGGATGTCCTGCCCACGCGAATACAGGCGCTGCACCTTCAGGGCGATAAt < 2:249623/150‑1 (MQ=255) ccGAGCAGCAGGC‑TTTCGGCCTCGGCCGAAAAGCGCAGGTTCGGCTTGCCATAGCGCCGGCTATGGTGGGCAAGGAAATGCCGCGCCAGTAGCAGGATGTCCTg < 2:148454/104‑1 (MQ=255) ccGAGCAGCAGGC‑TTTCGGCCTCGGCCGAAAAGCGCAGGTTCGGCTTGCCATAGCGCCGGCTATGGTGGGCAAGGAAATGCCGCGCCAGTAGCAGGATGTCCTg > 1:148454/1‑104 (MQ=255) cGAGCAGCAGGC‑TTTCGGCCTCGGCCGAAAAGCGCAGGTTCGGCTTGCCATAGCGCCGGCTATGGTGGGCAAGGAAATGCCGCGCCAGTAGCAGGATGTCCTGCCCACGCGAATACAGGCGCGGCACCTTCAGGGCGATAATGCGAAGGc < 2:1131676/150‑1 (MQ=255) gAGCAGCAGGC‑TTTCGGCCTCGGCCGAAAAGCGCAGGTTCGGCTTGCCATAGCGCCGGCTATGGTGGGCAAGGAAATGCCGCGCCAGTAGCAGGATGTCCTGCCCACGCGAATACAGGCGCGGCACCTTCAGGGCGATAATGCGAAGGCg > 2:534189/1‑150 (MQ=255) gAGCAGCAGGC‑TTTCGGCCTCGGCCGAAAAGCGCAGGTTCGGCTTGCCATAGCGCCGGCTATGGTGGGCAAGGAAATGCCGCGCCAGTAGCAGGATGTCCTGCCCACGCGAATACAGGCGCGGCACCTTCAGGGCGATAATGCGAAGGCg < 1:786585/150‑1 (MQ=255) cagcagGC‑TTTCGGCCTCGGCCGAAAAGCGCAGGTTCGGCTTGCCATAGCGCCGGCTATGGTGGGCAAGGAAATGCCGCGCCAGTAGCAGGATGTCCTGCCCACGCGAATACAGGCGCGGCACCTTCAGGGCGATAATGCGAAGGCGgaa < 1:1060244/150‑1 (MQ=255) cagcagGC‑TTTCGGCCTCGGCCGAAAAGCGCAGGTTCGGCTTGCCATAGCGCCGGCTATGGTGGGCAAGGAAATGCCGCGCCAGTAGCAGGATGTCCTGCCCACGCGAATACAGGCGCGGCACCTTCAGGGCGATAATGCGAAGGCGgaa < 1:534189/150‑1 (MQ=255) cagcagGC‑TTTCGGCCTCGGCCGAAAAGCGCAGGTTCGGCTTGCCATAGCGCCGGCTATGGTGGGCAAGGAAATGCCGCGCCAGTAGCAGGATGTCCTGCCCACGCGAATACAGGCGCGGCACCTTCAGGGCGATAATGCGAAGGCGgaa < 1:161734/150‑1 (MQ=255) cagGC‑TTTCGGCCTCGGCCGAAAAGCGCAGGTTCGGCTTGCCATAGCGCCGGCTATGGTGGGCAAGGAAATGCCGCGCCAGTAGCAGGATGTCCTGCCCACGCGAATACAGGCGCGGCACCTTCAGGGCGATAATGCGAAGGCGgaagaa < 2:180400/150‑1 (MQ=255) gcgcttttCGGCCGAGGCCGAAAAGCGCAGGTTCGGCTTGCCATAGCGCCGGCTATGGTGGGCAAGGAAATGCCGCGCCAGTAGCAGGATGTCCTGCCCACGCGAATACAGGCGCGGCACCTTCAggg > 2:334719/6‑128 (MQ=255) gcgcttttCGGCCGAGGCCGAAAAGCGCAGGTTCGGCTTGCCATAGCGCCGGCTATGGTGGGCAAGGAAATGCCGCGCCAGTAGCAGGATGTCCTGCCCACGCGAATACAGGCGCGGCACCTTCAggg < 1:334719/123‑1 (MQ=255) ggC‑TTTCGGCCTCGGCCGAAAAGCGCAGGTTCGGCTTGCCATAGCGCCGGCTATGGTGGGCAAGGAAATGCCGCGCCAGTAGCAGGATGTCCTGCCCACGCGAATACAGGCGCGGCACCTTCAGGGCGATAATGCGAAGGCGGAAGAACa > 1:916938/1‑150 (MQ=255) gcttttCGGCCGAGGCCGAAAGGCGCAGGTTCGGCTTGCCATAGCGCCGGCTATGGTGGGCAAGGAAATGCCGCGCCAGTAGCAGGATGTCCTGCCCACGCGAATACAGGCGCGGCACCTTCAGGGCGATAATGCGAAGGCGGAAGAACa > 2:171217/4‑150 (MQ=255) gC‑TTTCGGCCTCGGCCGAAAAGCGCAGGTTCGGCTTGCCATAGCGCCGGCTATGGTGGGCAAGGAAATGCCGCGCCAGTAGCAGGATGTCCTGCCCACGCGAATACAGGCGCGGc > 2:429243/1‑115 (MQ=255) gC‑TTTCGGCCTCGGCCGAAAAGCGCAGGTTCGGCTTGCCATAGCGCCGGCTATGGTGGGCAAGGAAATGCCGCGCCAGTAGCAGGATGTCCTGCCCACGCGAATACAGGCGCGGc < 1:429243/115‑1 (MQ=255) tcggccGAAAAGCGCAGGTTCGGCTTGCCATAGCGCCGGCTATGGTGGGCAAGGAAATGCCGCGCCAGTAGCAGGATGTCCTGCCCACGCGAATACAGGCGCGGCACCTTCAGGGCGATAATGCGAAGGCGGAAGAACAGGTCACGCCTg < 2:948783/150‑1 (MQ=255) | CGGCTCGTCAACCAAGGTCATGCACAGGTTCAACTGATGCGCTTGCACCACCTCGTTCGGCGCCAGCAACACCGTCTGCTCCAGCATGTTGCGCAATTCACGCACATTGCCAGGCCAGCTATAACCGAGCAGCAGGC‑TTTCGGCCTCGGCCGAAAAGCGCAGGTTCGGCTTGCCATAGCGCCGGCTATGGTGGGCAAGGAAATGCCGCGCCAGTAGCAGGATGTCCTGCCCACGCGAATACAGGCGCGGCACCTTCAGGGCGATAATGCGAAGGCGGAAGAACAGGTCACGCCTG > NC_002947/3869757‑3870051 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |