| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 3,983,800 | C→T | 6.1% | S257L (TCG→TTG) | ilvE → | branched‑chain‑amino acid aminotransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 3,983,800 | 0 | C | T | 6.1% | 192.8 / 2.8 | 65 | S257L (TCG→TTG) | ilvE | branched‑chain‑amino acid aminotransferase |
| Reads supporting (aligned to +/- strand): ref base C (29/32); new base T (2/2); total (31/34) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.83e-01 | |||||||||||
ACCTCGACCCGCTGACCCACACCAAGATCGAGGAAGTCGGTTCGGCCAACTTCTTCGGCATCACCGCCAACAACGAGTTCGTCACCCCGAAATCGGCCTCGGTACTGCCAGGCATCACCCGCCTCTCGCTGATGGAACTGGCGCAATCGCGCCTGGGCATGACCGTGATCGAAGGCGATGTCGAGATCAGCAAGCTGGACCGCTTCGTCGAAGCCGGTGCCTGCGGCACCGCGGCCGTTATCACCCCGATCGGCGGCATCGAGTACAACGGCAAGCTGCACGTGT > NC_002947/3983653‑3983937 | aCCTCGACCCGCTGACCCACACCAAGATCGAGGAAGTCGGTTCGGCCAACTTCTTCGGCATCACCGCCAACAACGAGTTCGTCACCCCGAAATCGGCCTCGGTACTGCCAGGCATCACCCGCCTCTCGCTGATGGAACTGGCGCAATcgc > 2:1071675/1‑150 (MQ=255) cTCGACCCGCTGACCCACACCAAGATCGAGGAAGTCGGCTCGGCCAACTTCTTCGGCATCACCGCCAACAACGAGTTCGTCACCCCGAAATCGGCCTCGGTACTGCCAGGCATCACCCGCCTCTCGCTGATGGAACTGGCGCAATCgcgc > 1:731403/1‑150 (MQ=255) tCGACCCGCTGACCCACACCAAGATCGAGGAAGTCGGTTCGGCCAACTTCTTCGGCATCACCGCCAACAACGAGTTCGTCACCCCGAAATCAGCCTCGGTACTGCCAGGCATCACCCGCCTCTCGCTGATGGAACTGGCGCAATCGCGcc > 1:726094/1‑150 (MQ=255) gACCCGCTGACCCACACCAAGCTCGAGGAAGTCGGTTCGGCCAACTTCTTCGGCATCACCGCCAACAACGAGTTCGTCACCCCGAAATCGGCCTCGGTACTGCCAGGCATCACCCGCCTCTCGCTGATGGAACTGGCGCAATCGCGCCTg < 1:573029/150‑1 (MQ=255) cccGCTGACCCACACCAAGATCGAGGAAGTCGGTTCGGCCAACTTCTTCGGCATCACCGCCAACAACGAGTTCGTCACCCCGAAATCGGCCTCGGTACTGCCAGGCATCACCCGCCTCTCGCTGATGGAACTGGCGCAATCGCGCCTggg < 1:488122/150‑1 (MQ=255) ccGCTGACCCACACCAAGATCGAGGAAGTCGGTTCGGCCAACTTCTTCGGCATCACCGCCAACAACGAGTTCGTCACCCCGAAATCAGCCTCGGTACTGCCAGGCATCACCCGCCTCTCGCTGATGGAACTGGCGCAATCGCGCCTGGGc < 2:726094/150‑1 (MQ=255) gACCCACACCAAGATCGAGGAAGCTGTCTCGGCCAACTTCTTCGGCATCACCGCCAACAACGAGTTCGTCACCCCGAAATCGGCCTCGGTACTGCCAGGCATCACCCGCCTCTCGCTGATGGAACTGGCGCAATCGCGCCTGGGCATGAc < 1:36/150‑1 (MQ=255) ccACACCAAGATCGAGGAAGTCGGTTCGGCCAACTTCTTCGGCATCACCGCCAACAACGAGTTCGTCACCCCGAAATCGGCCTCGGTACTGCCAGGCATCACCCGCCTCTCGCTGATGGAACTGGCGCAATCGCGCCTGGGCATGACCGt > 2:780614/1‑150 (MQ=255) acacCAAGATCGAGGAAGTCGGTTCGGCCAACTTCTTCGGCATCACCGCCAACAACGAGTTCGTCACCCCGAAATCGGCCTCGGTACTGCCAGGCATCACCCGCCTCTCGCTGATGGAACTGGCGCAATCGCGCCTGGGCATGACCGTGa < 1:780614/150‑1 (MQ=255) cAAGATCGAGGAAGTCGGTTCGGCCAACTTCTTCGGCATCACCGCCAACAACGAGTTCGTCACCCCGAAATCGGCCTCGGTACTGCCAGGCATCACCCGCCTCTCGCTGATGGAACTGGCGCAATCGCGCCTGGGCATGACCGTGATCGa > 1:498325/1‑150 (MQ=255) cAAGATCGAGGAAGTCGGTTCGGCCAACTTATTCGGCATCACCGCAATCGCAGGATTCGTCACCCCGAAATCGGCCTCGGTACTGCCAGGCATCACCCGCCTCTCGCTGATGGAACTGGCGCAAGCGCGCCGGGGCATGACCGTTATCGa > 2:470681/1‑150 (MQ=255) aGATCGAGGAAGTCGGCTCGGCCAACTTCTTCGGCATCACCGCCAACAACGAGTTCGTCACCCCGAAATCGGCCTCGGTACTGCCAGGCATCACCCGCCTCTCGCTGATGGAACTGGCGCAATCGCGCCTGGGCATGACCGTGATCGAAg < 2:731403/150‑1 (MQ=255) gATCGAGGAAGTCGGTTCTCCCAACTTCTTCGTCATCACCGCCAACAACGAGTTCGTCACCCCGAAATCGGCCTCGGTACTGCCAGGCATCACCCGCCTCTCGCTGATGGAACTGGCGCAATCGCGCCTGGGCATGACCGTGATCGAAgg < 2:568163/150‑1 (MQ=255) gAGGAAGTCGGTTCGGCCAACTTCTTCGGCATCACCGCCAACAACGAGTTCGTCACCCCGAAATCGGCCTCGGTACTGCCAGGCATCACCCGCCTCTCGCTGATGGAACTGGCGCAATCGCGCCTGGGCATGACCGTGATCGAAGGCGAt > 1:436175/1‑150 (MQ=255) ggAAGTCGGTTCGGCCAACTTCTTCGGCATCACCGCCAACAACGAGTTCGTCACCCCGAAATCGGCCTCGGTACTGCCAGGCATCACCCGCCTCTCGCTGATGGAACTGGCGCAATCGCGCCTGGGCATGGCCGTGATCGAAGGCGATGt > 2:427824/1‑150 (MQ=255) gTTCGGCCAACTTCTTCGGCATCACCGCCAACAACGAGTTCGTCACCCCGAAATCGGCCTCGGTACTGCCAGGCATCACCCGCCTCTCGCTGATGGAACTGGCGCAATCGCGCCTGGGCATGACCGTGATCGAAGGCGATGTCGAGATCa > 2:971937/1‑150 (MQ=255) gTTCGACCAAACTCCTCGGCATCACCGCCAACAACGAGTTCGTCACCCCGAAATCGGCCTCGGTACTGCCAGGCATCACCCGCCTCTCGCTGATCGAACTGGCGCAATCGCGCCTGGGCATGACCGTGATCGAAGGCGATGTCGAGATCa < 2:335820/150‑1 (MQ=255) ggCCAACTTCTTCGGCATCACCGCCAACAACGAGTTCGTCACCCCGAAATCGGCCTCGGTACTGCCAGGCATCACCCGCCTCTCGCTGATGGAACTGGCGCAATCGCGCCTGGGCATGACCGTGATCGAAGGCGATATCGAGATCAGCaa < 2:812866/150‑1 (MQ=255) aaCTTCTTCGGCATCACCGCCAACAACGAGTTCGTCACCCCGAAATCGGCCTCGGTACTGCCAGGCATCACCCGCCTCTCGCTGATGGAACTGGCGCAATCGCGCCTGGGCATGACCGTGATCGAAGGCGATGTCGAGATCAGCAAGCTg > 1:224341/1‑150 (MQ=255) aCTTCTTCGGCATCACCGCCAACAACGAGTTCGTCACCCCGAAATCGGCCTCGGTACTGCCAGGCATCACCCGCCTCTCGCTGATGGAACTGGCGCGATTGCGccagtt > 1:190195/1‑105 (MQ=255) aCTTCTTCGGCATCACCGCCAACAACGAGTTCGTCACCCCGAAATCGGCCTCGGTACTGCCAGGCATCACCCGCCTCTCGCTGATGGAACTGGCGCGATTGCGccagtt < 2:190195/109‑5 (MQ=255) cttcttCGGCATCACCGCCAACAACGAGTTCGTCACCCCGAAATCGGCCTCGGTACTGCCAGGCATCACCCGCCTCTCGCTGATGGAACTGGCGCAATCGCGCCTGGGCATGACCGTGATCGAAGGCGATGTCGAGATCAGCAAGCTGGa < 1:776219/150‑1 (MQ=255) ttcttcGGCATCACCGCCAACAACGAGTTCGTCACCCCGAAATCGGCCTCGGTACTGCCAGGCATCACCCGCCTCTCGCTGATGGAACTGGCGCAATCGCGCCTGGGCATGACCGTGATCGAAGGCGATGTCGAGATCAGCAAGCTGGAc < 2:909730/150‑1 (MQ=255) aCCGCCAACAACGAGTTCGTCACCCCGAAATCGGCCTCGGTACTGCCAGGCATCACCCGCCTCTCGCTGATGGAACTGGCGCGATTGCGcca > 1:815611/1‑91 (MQ=255) aCCGCCAACAACGAGTTCGTCACCCCGAAATCGGCCTCGGTACTGCCAGGCATCACCCGCCTCTCGCTGATGGAACTGGCGCGATTGCGcca < 2:815611/92‑2 (MQ=255) ccGCCAACAACGAGTTCGTCACCCCGAAATCGGCCTCGGTACTGCCAGGCATCACCCGCCTCTCGCTGATGGAACTGGCGCAATCGCGCCTGGGCATGACCGTGATCGAAGGCGATGTCGAGATCAGCAAGCTGGACCGCTTCGTCGAAg > 2:487475/1‑150 (MQ=255) cGCCAACAACGAGTTCGTCACCCCGAAATCGGCCTCGGTACTGCCAGGCATCACCCGCCTCTCGCTGATGGAACTGGCGCAATCGCGCCTGGGCATGACCGTGATCGAAGGCGATGTCGAGATCAGCAAGCTGGACCGCTTCGTCGAAGc > 2:898276/1‑150 (MQ=255) gCCAACAACGAGTTCGTCACCCCGAAATCGGCCTCGGTACTGCCAGGCATCACCCGCCTCTCGCTGATGGAACTGGCGCAATCGCGCCTGGGCATGACCGTGATCGAAGGCGATGTCGAGATCAGCAAGCTGGACCGCTTCGTCGAAGcc > 1:1003941/1‑150 (MQ=255) aacaacGAGTTCGTCACCCCGAAATCGGCCTCGGTACTGCCAGGCATCACCCGCCTCTCGCTGATGGAACTGGCGCAATCGCGCCTGGGCATGACCGTGATCGAAGGCGATGTCGAGATCAGCAAGCTGGACCGCTTCGTCGAAGCCGGt > 1:1146305/1‑150 (MQ=255) caacGAGTTCGTAACCCCGCAACCGGCCTAGGTACTGCCAGGCATCACCCGCCTCTCGCTGATGGAACTGGCGCAATCGCGCCTGGGCATGACCGTGATCGAAGGCGATGTCGAGATCAGCAAGCTGGACCGCTTCGTCGAAGCCGGTGc < 2:498325/150‑1 (MQ=255) aacGAGTTCGTCACCCCGAAATCGGCCTCGGTACTGCCAGGCATCACCCGCCTCTCGCTGATGGAACTGGCGCAATCGCGCCTGGGCATGACCGTGATCGAAGGCGATGTCGAGATCAGCAAGCTGGACCGCTTCGTCGa < 1:810975/140‑1 (MQ=255) aacGAGTTCGTCACCCCGAAATCGGCCTCGGTACTGCCAGGCATCACCCGCCTCTCGCTGATGGAACTGGCGCAATCGCGCCTGGGCATGACCGTGATCGAAGGCGATGTCGAGATCAGCAAGCTGGACCGCTTCGTCGa > 2:810975/1‑140 (MQ=255) cGAGTTCGTCACCCCGAAATCGGCCTCGGTACTGCCAGGCATCACCCGCCTCTCGCTGATGGAACTGGCGCAATCGCGCCTGGGCATGACCGTGATCGAAGGCGATGTCGAGATCAGCAAGCTGGACCGCTTCGTCGAAGCCgg > 2:539503/1‑144 (MQ=255) cGAGTTCGTCACCCCGAAATCGGCCTCGGTACTGCCAGGCATCACCCGCCTCTCGCTGATGGAACTGGCGCAATCGCGCCTGGGCATGACCGTGATCGAAGGCGATGTCGAGATCAGCAAGCTGGACCGCTTCGTCGAAGCCgg < 1:539503/144‑1 (MQ=255) gTTCGTCACCCCGAAATCGGCCTCGGTACTGCCAGGCATCACCCGCCTCTCGCTGATGGAACTGGCGCAATCGCGCCTGGGCATGACCGTGATCGAAGGCGATGTCGAGATCAGCAAGCTGGACCGCTTCGTCGAAGCCGGTGCCTGCgg < 2:224341/150‑1 (MQ=255) cccGAAATCGGCCTCGGTACTGCCAGGCATCACCCGCCTCTCGCTGATGGAACTGGCGCAATCGCGCCTGGGc < 2:817244/73‑1 (MQ=255) cccGAAATCGGCCTCGGTACTGCCAGGCATCACCCGCCTCTCGCTGATGGAACTGGCGCAATCGCGCCTGGGc > 1:817244/1‑73 (MQ=255) aaaTCGGCCTCGGTACTGCCAGGCATCACCCGCCTCTCGCTGATGGAACTGGCGCAATCGCGCCTGGGCATGACCGTGATCGAAGGCGATGTCGAGATCAGCAAGCTGGACCGCTTCGTCGAAGCCGGTGCCTGCGGCACCGCGGCCGtt < 1:898276/150‑1 (MQ=255) ggCCTCGGTACTGCCAGGCATCACCCGCCTCTCGCTGATGGAACTGGCGCAATCGCGCCTGGGCATGACCGTGATCGAAGGCGATGTCGAGATCAGCAAGCTGGACCGCTTCGTCGAAGCCGGTGCCTGCGGCACCGCGGCCGTTATCAc < 1:1071675/150‑1 (MQ=255) ggCCTCGGTACTGCCAGGCATCACCCGCCTCTCGCTGATGGAACTGGCGCAATCGCGCCTGGGCATGACCGTGATCGAAGGCGATGTCGAGATCAGCAAGCTGGACCGCTTCGTCGAAGCCGGTGCCTGCGGCACCGCCGCCGCTATCAc > 2:305678/1‑150 (MQ=255) gTACTGCCAGGCATCACCCGCCTCTCGCTGATGGAACTGGCGCAATCGCGCCTGGGCATGACCGTGATCGAAGGCGATGTCGAGATCAGCAAGCTGGACCGCTTCGTCGAAGCCGGTGCGTGCGGCACCGCGGCCGTTATCACCCCGATc > 1:319814/1‑150 (MQ=255) tGCCAGCCATCACCCGCCTCTCGCTGATGGAACTGGCGCAATCGCGCCTGGGCATGACCGTGATCGAAGGCGATGTCGAGATCAGCAAGCTGGACCGCTTCGTCGAAGCCGGTGCCTGCGGCACCGCGGCCGTTATCACCCCGATcggcg < 1:470681/150‑1 (MQ=255) gCCAGGCATCACCCGCCTCTCGCTGATGGAACTGGCGCAATCGCGCCTGGGCATGACCGTGATCGAAGGCGAt > 2:462091/1‑73 (MQ=255) gCCAGGCATCACCCGCCTCTCGCTGATGGAACTGGCGCAATCGCGCCTGGGCATGACCGTGATCGAAGGCGAt < 1:462091/73‑1 (MQ=255) ccAGGCATCACCCGCCTCTCGCTGATGGAACTGGCGCAATCGCGCCTGGGCATGACCGTGATCGAAGGCGATGTCGAGATCAGCAAGCTGGACCGCTTCGTCGAAGCCGGTGCCTGCGGCACCGCGGCCGTTATCACCCCGATCggcggc > 1:746570/1‑150 (MQ=255) cAGGCATCACCCGCCTCTCGCTGATGGAACTGGCGCAATCGCGCCTGGGCATGACCGTGATCGAAGGCGATGTCGAGATCAGCAAGCTGGACCGCTTCGTCGAAGCCGGTGCCTGCGGCACCGCGGCCGTTATCa > 2:202099/1‑135 (MQ=255) cAGGCATCACCCGCCTCTCGCTGATGGAACTGGCGCAATCGCGCCTGGGCATGACCGTGATCGAAGGCGATGTCGAGATCAGCAAGCTGGACCGCTTCGTCGAAGCCGGTGCCTGCGGCACCGCGGCCGTTATCa < 1:202099/135‑1 (MQ=255) cATCACCCGCCTCTCGCTGATGGAACTGGCGCAATCGCGCCTGGGCATGACCGTGATCGAAGGCGATGTCGAGATCAGCAAGCTGGACCGCTTCGTCGAAGCCGGTGCCTGCGGCACCGCGGCCGTTATCACCCCGATCGGCGGCATCGa < 2:1003941/150‑1 (MQ=255) tCACCCGCCTCTCGCTGATGGAACTGGCGCAATCGCGCCTGGGCATGGCCGTGATCGAAGGCGATGTCGAGATCAGCAAGCTGGACCGCTTCGTCGAAGCCGGTGCCTGCGGCACCGCGGCCGTTATCACCCCGATCGGCGGCATCGAGt < 1:427824/150‑1 (MQ=255) cccGCCTCTCGCTGATGGAACTGGCGCAATCGCGCCTGGGCATGACCGTGATCGAAGGCGATGTCGAGATCAGCAAGCTGGACCGCTtcgt > 2:517081/1‑91 (MQ=255) cccGCCTCTCGCTGATGGAACTGGCGCAATCGCGCCTGGGCATGACCGTGATCGAAGGCGATGTCGAGATCAGCAAGCTGGACCGCTtcgt < 1:517081/91‑1 (MQ=255) cccGCCTCTCGCTGATGGAACTGGCGCAATCGCGCCTGGGCATGACCGTGATCGAAGGCGATGTCGAGATCAGCAAGCTGGACCGCTTCGTCGAAGCCGGTGCCTGCGGCACCGCGGCCGTTATCACCCCGATCGGCGGCATCGAGTACa > 2:855235/1‑150 (MQ=255) ccGCCTCTCGCTGATGGAACTGGCGCAATCGCGCCTGGGCATGACCGTGATCGAAGGCGATGTCgaga < 1:825941/68‑1 (MQ=255) ccGCCTCTCGCTGATGGAACTGGCGCAATCGCGCCTGGGCATGACCGTGATCGAAGGCGATGTCgaga > 2:825941/1‑68 (MQ=255) gCCTCTCGCTGATGGAACTGGCGCAATCGCGCCTGGGCATGACCGTGATCGAAGGCGATGTCGAGATCAGCAAGCTGGACCGCTTCGTCGAAGCCGGTGCCTGCGGCACCGCGGCCGTTATCACCCCGATCGGCGGCATCGAGTACAACg < 1:971937/150‑1 (MQ=255) ctctCGCTGATGGAACTGGCGCAATCGCGCCTGGGCATGACCGTGATCGAAGGCGATGTCGAGATCAGc < 2:781988/69‑1 (MQ=255) ctctCGCTGATGGAACTGGCGCAATCGCGCCTGGGCATGACCGTGATCGAAGGCGATGTCGAGATCAGc > 1:781988/1‑69 (MQ=255) tctcGCTGATGGAACTGGCGCAATCGCGCCTGGGCATGACCGTGATCGAAGGCGATGTCGAGATCAGCAAGCTGGACCGCTTCGTCGaa < 2:1035354/89‑1 (MQ=255) tctcGCTGATGGAACTGGCGCAATCGCGCCTGGGCATGACCGTGATCGAAGGCGATGTCGAGATCAGCAAGCTGGACCGCTTCGTCGaa > 1:1035354/1‑89 (MQ=255) tctcGCTGATGGAACTGGCGCAATCGCGCCTGGGCATGACCGTGATCGAAGGCGATGTCGAGATCAGCAAGCTGGACCGCTTCGTCGAAGCCGGTGCCTGCGGCACCGCGGCCGTTATCACCCCGATCGGCGGCATCGAGTACAACGGCa > 2:33405/1‑150 (MQ=255) tctcGCTGATGGAACTGGCGCAATCGCGCCTGGGCATGACCGTGATCGAAGGCGATGTCGAGATCAGCAAGCTGGACCGCTTCGTCGAAGCCGGTGCCTGCGGCACCGCGGCCGTTATCACCCCGATCGGCGGCATCGAGTACAACGGCa > 1:293027/1‑150 (MQ=255) ctcGCTGATGGAACTGGCGCAATCGCGCCTGGGCATGACCGTGATCGAAGGCGATGTCGAGATCAGCAAGCTGGACCGCTTCGTCGAAGCCGGTGCCTGCGGCACCGCGGCCGTTATCACCCCGATCGGCGGCATCGAGTACAACGGCaa < 2:1146305/150‑1 (MQ=255) tcGCTGATGGAACTGGCGCAATCGCGCCTGGGCATGACCGTGATCGAAGGCGATGTCGAGATCAGCAAGCTGGACCGCTTCGTCGAAGCCGGTGCCTGCGGCACCGCGGCCGTTATCACCCCGATCGGCGGCATCGAGTACAACGGCAAg < 1:33405/150‑1 (MQ=255) aaCTGGCGCAATCGCGCCTGGGCATGACCGTGATCGAAGGCGATGTCGAGATCAGCAAGCTGGACCGCTTCGTCGAAGCCGGTGCCTGCGGCACCGCGGCCGTTATCACCCCGATCGGCGGCATCGAGTACAACGGCAAGCTGCACgtgt < 1:855235/150‑1 (MQ=255) gcgcAATCGCGCCTGGGCATGACCGTGATCGAAGGCGATGTCGAGATCAGCAAGCTg > 2:253707/1‑57 (MQ=255) gcgcAATCGCGCCTGGGCATGACCGTGATCGAAGGCGATGTCGAGATCAGCAAGCTg < 1:253707/57‑1 (MQ=255) | ACCTCGACCCGCTGACCCACACCAAGATCGAGGAAGTCGGTTCGGCCAACTTCTTCGGCATCACCGCCAACAACGAGTTCGTCACCCCGAAATCGGCCTCGGTACTGCCAGGCATCACCCGCCTCTCGCTGATGGAACTGGCGCAATCGCGCCTGGGCATGACCGTGATCGAAGGCGATGTCGAGATCAGCAAGCTGGACCGCTTCGTCGAAGCCGGTGCCTGCGGCACCGCGGCCGTTATCACCCCGATCGGCGGCATCGAGTACAACGGCAAGCTGCACGTGT > NC_002947/3983653‑3983937 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |