Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,082,776 C→G 8.5% A226G (GCG→GGG)  PP_3592 → RpiR family transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,082,7760CG 8.5% 145.6 / 4.1 47A226G (GCG→GGG) PP_3592RpiR family transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base C (20/23);  new base G (2/2);  total (22/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.84e-01

GGCGTGCAACTGGTCGACATCAGCGCCGGGCACTTCGGCGAAGTGCTGCTGGGCCGTGCCGAAGACAGCGCCCTGGTGGTGTTCGAGGCGCGCCGTTATTCCCGCCATGCCCTGATGCTGTGCCAGAAGGCGCGCGAGGCTGGCATTGCGGTCACCCTGGTGACCGACACCTTCTGTGACTGGGCGGATGCCAATGCCGACGAGGTGTTCCGCATCCCCACCGAGTTCAACCTGTTCTGGGAATCCACCTCGACGATGCTGTCGTGGGTGCATCTGATGGTCAACGAGGTCT  >  NC_002947/4082628‑4082919
                                                                                                                                                    |                                                                                                                                               
ggCGTGCAACTGGTCGACATCAGCGCCGGGCACTTCGGCGAAGTGCTGCTGGGCCGTGCCGAAGACAGCGCCCTGGTGGTGTTCGAGGCGCGCCGTTATTCCCGCCATGCCCTGATGCTTTGCCAGAAGGCGCGCGAGGCTGGCATTGCg                                                                                                                                                <  1:596877/150‑1 (MQ=255)
    ggCAACTGGTCGACATCAGCGCCGGGCACTTCGGCGAAGTGCTGCTGGGCCGTGCCGAAGACAGCGCCCTGGTGGTGTTCGAGGCGCGCCGTTATTCCCGCCATGCCCTGATGCTGTGCCAGAAGGCGCGCGAGGCTGGCATTGCGGTCa                                                                                                                                            <  2:592647/149‑1 (MQ=255)
       aaCTGGTCGACATCAGCGCCGGGCACTTCGGCGAAGTGCTGCTGGGCCGTGCCGAAGACAGCGCCCTGGTGGTGTTCGAGGCGCGCCGTTATTCCCGCCATGCCCTGATGCTGTGCCAGAAGGCGCGCGAGGCTGGCATTGCGGTCAccc                                                                                                                                         >  2:1128131/1‑150 (MQ=255)
          tGGTCGACATCAGCGCCGGGCACTTCGGCGAAGTGCTGCTGGGCCGTGCCGAAGACAGCGCCCTGGTGGTGTTCGAGGCGAGCCGTTATTCCCGCCATGCCCTGATGCTGTGCCAGAAGGCGCGCGAGGCTGGCATTGGGGTCACCCTgg                                                                                                                                      >  1:75601/1‑150 (MQ=255)
             tCGACATCAGCGCCGGGCACTTCGGCGAAGTGCTGCTGGGCCGTGCCGAAGACAGCGCCCTGGTGGTGTTCGAGGCGCGCCGTTATTCCCGCCATGCCCTGATGCTGTGCCAGAAGGCGCGCGAGGCTGGCATTGCGGTCACCCTGGTGa                                                                                                                                   >  1:612163/1‑150 (MQ=255)
               gACATCAGCGCCGGGCACTTCGGCGAAGTGCTGCTGGGCCGTGCCGAAGACAGCGCCCTGGTGGTGTTCGAGGCGCGCCGTTATTCCCGCCATGCCCTGATGCTGTGCCAGAAGGCGCGCGAGGCTGGCATTGCGGTCACCCTGGTGAcc                                                                                                                                 >  1:518536/1‑150 (MQ=255)
                 cATCAGCGCCGGGCACTTCGGCGAAGTGCTGCTGGGCCGTGCCGAAGACAGCGCCCTGGTGGTGTTCGAGGCGCGCCGTTATTCCCGCCATGCCCTGATGCTGTGCCAGAAGGCGCGCGAGGCTGGCATTGCGGTCACCCTGGTGACCGa                                                                                                                               <  1:1128131/150‑1 (MQ=255)
                   tCAGCGCCGGGCACTTCGGCGAAGTGCTGCTGGGCCGTGCCGAAGACAGCGCCCTGGTGGTGTTCGAGGCGCGCCGTTATTCCCGCCATGCCCTGATGCTGTGCCAGAAGGCGCGCGAGGCTGGCATTGCg                                                                                                                                                >  2:530090/1‑131 (MQ=255)
                   tCAGCGCCGGGCACTTCGGCGAAGTGCTGCTGGGCCGTGCCGAAGACAGCGCCCTGGTGGTGTTCGAGGCGCGCCGTTATTCCCGCCATGCCCTGATGCTGTGCCAGAAGGCGCGCGAGGCTGGCATTGCg                                                                                                                                                <  1:530090/131‑1 (MQ=255)
                      gcgcCGGGCACTTCGGCGAAGTGCTGCTGGGCCGTGCCGAAGACAGCGCCCTGGTGGTGTTCGAGGCGCGCCGTTATTCCCGCCATGCCCTGATGCTGTGCCAGAAGGCGCGCGAGGCTGGCATTGCGGTCACCCTGGTGACCGACACCt                                                                                                                          >  1:422969/1‑150 (MQ=255)
                      gcgcCGGGCACTTCGGCGAAGTGCTGCTGGGCCGTGCCGAAGACAGCGCCCTGGTGGTGTTCGAGGCGCGCCGTTATTCCCGCCATGACCTGATGCTGTGCCAGAAGGCGCGCGAGGCTGGCAGTGCGGTCACCCTGGTGACCGACACCt                                                                                                                          <  2:741014/150‑1 (MQ=255)
                       cgcCGGGCACTTCGGCGAAGTGCTGCTGGGCCGTGCCGAAGACAGCGCCCTGGTGGTGTTCGAGGCGCGCCGTTATTCCCGCCATGCCCTGATGCTGTGCCAGAAGGCGCGCGAGGCTGGCATTGCGGTCACCAGGGTGACCgcaatgcc                                                                                                                         <  1:551013/150‑8 (MQ=255)
                           gggCACTTCGGCGAAGTGCTGCTGGGCCGTGCCGAAGACAGCGCCCTGGTGGTGTTCGAGGCGCGCCGTTATTCCCGCCATGCCCTGATGCTGTGCCAGAAGGCGCGCGAGGCTGGCATTGGGGTCACCCTGGTGACCGACACCTTCtgt                                                                                                                     <  2:75601/150‑1 (MQ=255)
                                 ttCGGCGAAGTGCTGCTGGGCCGTGCCGAAGACAGCGCCCTGGTGGTGTTCGAGGCGCGCCGTTATTCCCGCCATGCCCTGATGCTGTGCCAGAAGGCGCGCGAGGCTGGCATTGGGGt                                                                                                                                              >  2:598673/1‑119 (MQ=255)
                                 ttCGGCGAAGTGCTGCTGGGCCGTGCCGAAGACAGCGCCCTGGTGGTGTTCGAGGCGCGCCGTTATTCCCGCCATGCCCTGATGCTGTGCCAGAAGGCGCGCGAGGCTGGCATTGGGGt                                                                                                                                              <  1:598673/119‑1 (MQ=255)
                                 ttCGGCGAAGTGCTGCTGGGCCGTGCCGAAGACAGCGCCCTGGTGGTGTTCGAGGCGCGCCGTTATTCCCGCCATGCCCTGATGCTGTGCCAGAAGGCGCGCGAGGCTGGCATTGCGGTCACCCTGGTGACCGACACCTTCTGTGACTgg                                                                                                               <  2:1196311/150‑1 (MQ=255)
                                 ttCGGCGAAGTGCTGCTGGGCCGTGCCGAAGACAGCGCCCTGGTGGTGTTCGAGGCGCGCCGTTATTCCCGCCATGCCCTGATGCTGTGCCAGAAGGCGCGCGAGGCTGGCATTGCGGTCACCCTGGTGACCGACACCTTCTGTGACTgg                                                                                                               >  2:7298/1‑150 (MQ=255)
                                   cGGCGAAGTGCTGCTGGGCCGTGCAGAAGACAGCGCCCTGGTGGTGTTCGAGGCGCGCCGTTATTCCCGCCATGCCCTGATGCTGTGCCAGAAGGCGCGCGAGGCTGGCATGGCGGTCACCCTGGTGACCGACACCTTCTGTGACTGGGc                                                                                                             <  2:467519/150‑1 (MQ=255)
                                       gAAGTGCTGCTGGGCCGTGCCGAAGACAGCGCCCTGGTGGTGTTCGAGGCGCGCCGTTATTCCCGCCATGCCCTGATGCTGTGCCAGAAGGCGCGCGAGGCTGGCATTGCGGTCACCCTGGTGACCGACACCTTCTGTGACTGGGCGGAt                                                                                                         <  2:518536/150‑1 (MQ=255)
                                        aaGTGCTGCTGGGCCGTGCCGAAGACAGCGCCCTGGTGGTGTTCGAGGCGCGCCGTTATTCCCGCCATGCCCTGATGCTGTGCCAGAAGGCGCGCGAGGCTGGCATTGCGGTCACCCTGGTGACCGACACCTTCTGTGACTGGGCGGATg                                                                                                        <  1:490832/150‑1 (MQ=255)
                                             ctgctgGGCCGTGCCGAAGACAGCGCCCTGGTGGTGTTCGAGGCGCGCCGTTATTCCCGCCATGCCCTGATGCTGTGCCAGAAGGCGCGCGAGGCTGGCATTGCGGTCACCCTGGTGACCGACACCTTCTGTGACTGGGCGGATGCCAAt                                                                                                   >  1:188795/1‑150 (MQ=255)
                                                      cGTGCCGAAGACAGCGCCCTGGTGGTGTTCGAGGCGCGCCGTTATTCCCGCCATGCCCTGATGCTGTGCCAGAAGGCGCGCGAGGCTGGCATTGCGGTCACCCTGGTGACCGACACCTTCTGTGACTGGGCGGATGCCAATGCCGACGAg                                                                                          <  2:422969/150‑1 (MQ=255)
                                                          ccGAAGACAGCGCCCTGGTGGTGTTCGAGGCGCGCCGTTATTCCCGCCATGCCCTGATGCTGTGCCAGAAGGCGCGCGAGGCTGGCATTGCGGTCACCCTGGTGACCGACACCTTCTGTGACTGGGCGGATGCCAATGCCGACGAGgtgt                                                                                      >  1:852288/1‑150 (MQ=255)
                                                            gAAGACAGCGCCCTGGTGGTGTTCGAGGCGCGCCGTTATTCCCGCCATGCCCTGATGCTGTGCCAGAAGGCGCGCGAGGCTGGCATTGCGGTCACCCTGGTGACCGACACCTTCTGTGACTGGGCGGATGCCAATGCCGACGAGGTGTTc                                                                                    <  2:340835/150‑1 (MQ=255)
                                                            gAAGACAGCGCCCTGGTGGTGTTCGAGGCGCGCCGTTATTCCCGCCATGCCCTGATGCTGTGCCAGAAGGCGCGCGAGGCTGGCATTGCGGTCACCCTGGTGACCGACACCTTCTGTGACTGGGCGGATGCCAATGCCGACGAGGTGTTc                                                                                    >  2:1117092/1‑150 (MQ=255)
                                                                    cgcCCTGGTGGTGTTCGAGGCGCGCCGTTATTCCCGCCATGCCCTGATGCTGTGCCAGAAGGCGCGCGAGGCTGGCATTGCGGTCACCCTg                                                                                                                                       >  2:1015344/1‑91 (MQ=255)
                                                                    cgcCCTGGTGGTGTTCGAGGCGCGCCGTTATTCCCGCCATGCCCTGATGCTGTGCCAGAAGGCGCGCGAGGCTGGCATTGCGGTCACCCTg                                                                                                                                       <  1:1015344/91‑1 (MQ=255)
                                                                           gtggtgTTCGAGGCGCGCCGTTATTCCCGCCATGCCCTGATGCTGTGCCAGAAGGCGCGCGAGGCTGGCATTGCGGTCACCCTGGTGACCGACACCTTCTGTGACTGGGCGGATGCCAATGCcgacga                                                                                           >  2:898539/1‑128 (MQ=255)
                                                                           gtggtgTTCGAGGCGCGCCGTTATTCCCGCCATGCCCTGATGCTGTGCCAGAAGGCGCGCGAGGCTGGCATTGCGGTCACCCTGGTGACCGACACCTTCTGTGACTGGGCGGATGCCAATGCcgacga                                                                                           <  1:898539/128‑1 (MQ=255)
                                                                              gtgtTCGAGGCGCGCCGTTATTCCCGCCATGCCCTGATGCTGTGCCAGAAGGCGCGCGAGGCTGGCATTGCGGTCACCCTGGTGACCGACACCTTCTGTGACTGGGCGGATGCCAATGCCGACGAGGTGTTCCGCATCCCCACCGAGTTc                                                                  >  2:843472/1‑150 (MQ=255)
                                                                                    gAGGCGCGCCGTTATTCCCGCCATGCCCTGATGCTGTGCCAGAAGGCGCGCGAGGCTGGCATTGCGGTCACCCTGGTGACCGACACCTTCTGTGACTGGGCGGATGCCAATGCCGACGAGGTGTTCCGCATCCCCACCGAGTTCAACCTg                                                            <  1:843472/150‑1 (MQ=255)
                                                                                          cgcCGTTATTCCCGCCATGCCCTGATGCTGTGCCAGAAGGCGCGCGAGGCTGGCATTGCGGTCACCCTGGTGACCGACACCTTCTGTGACTGGGCGGATGCCAATGCCGACGAGGTGTTCCGCATCCCCACCGAGTTCAACCTGTTCTgg                                                      <  2:852288/150‑1 (MQ=255)
                                                                                                    cccGCCATGCCCTGATGCTGTGCCAGAAGGCGCGCGAGGCTGGCATTGCGGTCACCGTAGCGGCCGACACCTTCTGTGACTGGGCGGATGCCAATGCCGACGAGGTGTTCCGCATCCCCACCGAGTTCAACCTGTTCTGGGAATCCACCt                                            >  2:1069465/1‑150 (MQ=255)
                                                                                                     ccGCCATGCCCTGCTGCTGTACCAGAAGGCGCGCGAGGCTGGCATTGCGGGCACCCTGGTGACCGACACCTTCTGTGACTGGGCGGATGCCAATGCCGACGAGGTGTTCCGCATCCCCACCGAGTTCAACCTGTTCTGGGAATCCACCTc                                           >  2:396984/1‑150 (MQ=255)
                                                                                                     ccGCCATGCCCTGATGCTGTGCCAGAAGGCGCGCGAGGCTGGCATTGCGGTCACCCTGGTGACCGACACCtt                                                                                                                         >  2:351983/1‑72 (MQ=255)
                                                                                                     ccGCCATGCCCTGATGCTGTGCCAGAAGGCGCGCGAGGCTGGCATTGCGGTCACCCTGGTGACCGACACCtt                                                                                                                         <  1:351983/72‑1 (MQ=255)
                                                                                                     ccGCCATGCCCTGATGCTGTGCCAGAAGGCGCGCGAGGCTGGCATTGCGGTCACCCTGGTGACCGACACCTTCTGTGACTGGGCGGATGCAAATGCCGACGAg                                                                                          >  2:529461/1‑103 (MQ=255)
                                                                                                     ccGCCATGCCCTGATGCTGTGCCAGAAGGCGCGCGAGGCTGGCATTGCGGTCACCCTGGTGACCGACACCTTCTGTGACTGGGCGGATGCAAATGCCGACGAg                                                                                          <  1:529461/103‑1 (MQ=255)
                                                                                                              ccTGATGCTGTGCCAGAAGGCGCGCGAGGCTGGCATTGCGGTCACCCTGGTGACCGACACCTTCTGTGACTGGGCGGATGCCAATGCCGACGAGGTGTTCCGCATCCCCACCGAGTTCAACCTGTTCTGGGAATCCACCTCGACGATGCt                                  >  1:467234/1‑150 (MQ=255)
                                                                                                                 gATGCTGTGCCAGAAGGCGCGCGAGGCTGGCATTGCGGTCACCCTGGTGACCGACACCTTCTGTGACTGGGCGGATGCCAATGCCGACGAGGTGTTCCGCATCCCCACCGAGTTCAACCTGTTCTGGGAATCCACCTCGACGATGCTGTc                               <  1:1089561/150‑1 (MQ=255)
                                                                                                                     cTGTGCCAGAAGGCGCGCGAGGCTGGCATTGCGGTCACCCTGGTGACCGACACCTTCTGTGACTGGGCGGATGCCAATGCCGACGAGGTGTTCCGCATCCCCACCGAGTTCAACCTGTTCTGGGAATCCACCTCGACGATGCTGTCGTgg                           <  1:43897/150‑1 (MQ=255)
                                                                                                                                ggCGCGCGAGGCTGGCATTGCGGTCACCCTGGTGACCGACACCTTCTGTGACTGGGCGGATGCCAATGCcga                                                                                              <  2:599669/72‑1 (MQ=255)
                                                                                                                                ggCGCGCGAGGCTGGCATTGCGGTCACCCTGGTGACCGACACCTTCTGTGACTGGGCGGATGCCAATGCcga                                                                                              >  1:599669/1‑72 (MQ=255)
                                                                                                                                   gcgcgAGGCTGGCATTGCGGTCACCCTGGTGACCGACACCTTCTGTGACTGGGCGGATGCCAATGCCGACGAGGTGTTCCGCATCCCCACCGAGTTCAACCTGTTCTGGGAATCCACCTCGACGATGCTGTCGTGGGTGCATCTGATGGt             >  2:383297/1‑150 (MQ=255)
                                                                                                                                      cgAGGCTGGCATTGCGGTCACCCTGGTGACCGACACCTTCTGTGACTGGGCGGATGCCAATGCCGACGAGGTGTTCCGCATCCCCACCGAGTTCAACCTGTTCTGGGAATCCACCTCGACGATGCTGTCGTGGGTGCATCTGATGGTCaa          <  2:467234/150‑1 (MQ=255)
                                                                                                                                            tGGCATTGCGGTCACCCTGGTGACCGACACCTTCTGTGACTGGGCGGATGCCAATGCCGACGAGGTGTTCCGCATCCCCACCGAGTTCAACCTGTTCTGGGAATCCACCTCGACGATGCTGTCGTGGGTGCATCTGATGGTCAACGAGGt    >  1:554237/1‑150 (MQ=255)
                                                                                                                                              gCATTGCGGTCACCCTGGTGACCGACACCTTCTGTGACTGGGCGGATGCCAATGCCGACGAGGTGTTCCGCATCCCCACCGAGTTCAACCTGTTCTGGGAATCCACCTCGACGATGCTGTCGTGGGTGCATCTGATGGTCAACGAGGTCt  <  1:7298/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                               
GGCGTGCAACTGGTCGACATCAGCGCCGGGCACTTCGGCGAAGTGCTGCTGGGCCGTGCCGAAGACAGCGCCCTGGTGGTGTTCGAGGCGCGCCGTTATTCCCGCCATGCCCTGATGCTGTGCCAGAAGGCGCGCGAGGCTGGCATTGCGGTCACCCTGGTGACCGACACCTTCTGTGACTGGGCGGATGCCAATGCCGACGAGGTGTTCCGCATCCCCACCGAGTTCAACCTGTTCTGGGAATCCACCTCGACGATGCTGTCGTGGGTGCATCTGATGGTCAACGAGGTCT  >  NC_002947/4082628‑4082919

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑