Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,133,672 G→T 7.8% A114D (GCC→GAC)  PP_3637 ← sulfonate ABC transporter ATP‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,133,6720GT 7.8% 157.1 / 3.5 52A114D (GCC→GAC) PP_3637sulfonate ABC transporter ATP‑binding protein
Reads supporting (aligned to +/- strand):  ref base G (24/23);  new base T (2/2);  total (27/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.68e-01

CCAGCAACAAGACTTCGGGCTGGCGTGCCAGGCAGCGGGCCAGCGCGGTGCGCTGGGCCATGCCGCCAGACAACTGCGCCGGGTACTGGCCACGGGCGTGGGCCAGGCCCACAGCCTCAATCGCATGGTCGATGCGTGCACGGCGTTCGGCATCCGCCAGCCTGGGCTGACGGGCGAAGTCCAGGCCGAAGGCGACGTTTTTCTCCAGGCTCAGCCATGGCAGCAGGCTGGGATCCTGAAAAGCCACCGCCAGACGCGGGTGCGGCCCCTGCAGCGGCTGGCCGTGCAAGCTCACGCT  >  NC_002947/4133523‑4133820
                                                                                                                                                     |                                                                                                                                                    
ccAGCAACAAGACTTCGGGCTGGCGTGCCAGGCAGCGGGCCAGCGCGGTGCGCTGGGCCATGCCGCCAGACAACTGCGCCGGGTACTGGCCACGGGCGTGGGCCAGGCCCACAGCCTCAATCGCATGGTCGATGCGTGCACGGCGTTCgg                                                                                                                                                      >  1:987631/1‑150 (MQ=255)
       caaGACTTCGGGCTGGCGTGCCAGGCAGCGGGCCAGCGCGGTGCGCTGGGCGATGCCGCCAGACAACTGCGCCGGGTACTGGCCACGGGCGTGGGCCAGGCCCACAGCCTCAATCGCATCGTCCATCAGTACACGACCATCGAGATCCGc                                                                                                                                               >  2:68238/1‑150 (MQ=255)
       caaGACTTCGGGCTGGCGTGCCAGGCAGCGGGCCAGCGCGGTGCGCTGGGCCATGCCGCCAGACAACTGCGCCGGGTACTGGCCACGGGCGTGGGCCAGGCCCACAGCCTCAATCGCATGGTCGATGCGTGCACGGCGTTCGGCATCCGc                                                                                                                                               >  2:1206924/1‑150 (MQ=255)
           aCTTCGGGCTGGCGTGCCAGGCAGCGGGCCAGCGCGGTGCGCTGGGCCATGCCGCCAGACAACTGCGCCGGGTACTGGCCACGGGCGTGGGCCAGGCCCACAGCCTCAATCGCATGGTCGATGCGTGCACGGCGTTCGGCATCCGCCAGc                                                                                                                                           <  2:711735/150‑1 (MQ=255)
             ttCGGGCTGGCGTGCCAGGCAGCGGGCCAGCGCGGTGCGCTGGGCCATGCCGCCAGACAACTGCGCCGGGTACTGGCCACGGGCGTGGGCCAGGCCCACAGCCTCAATCGCATGGTCGATGCGTGCACGGCGTTCGGCATCCGCCAGCCt                                                                                                                                         >  1:298002/1‑150 (MQ=255)
                 ggCTGGCGTGCCAGGCAGCGGGCCAGCGCGGTGCGCTGGGCCATGCCGCCAGACAACTGCGCCGGGTACTGGCCACGGGCGTGGGCCAGGCCCACAGCCTCAATCGCATGGTCGATGCGTGCACGGCGTTCGGCATCCGCCAGCCTGGGc                                                                                                                                     <  1:1016152/150‑1 (MQ=255)
                  gCTGGCGTGCCAGGCAGCGGGCCAGCGCGGTGCGCTGGGCCATGCCGCCAGACAACTGCGCCGGGTACTGGCCACGGGCGTGGGCCAGGCCCACAGCCTCAATCGCATGGTCGATGCGTGCACGGCGTTCGGCATCCGCCAGCCTGGGCt                                                                                                                                    >  2:714693/1‑150 (MQ=255)
                     ggCGTGCCAGGCAGCGGGCCAGCGCGGTGCGCTGGGCCATGCCGCCAGACAACTGCGCCGGGTACTGGCCACGGGCGTGGGCCAGGCCCACAGCCTCAATCGCATGGTCGATGCGTGCACGGCGTTCGGCATCCGCCAGCCTGGGCTGAc                                                                                                                                 <  1:595885/150‑1 (MQ=255)
                       cGTGCCAGGCAGCGGGCCAGCGCGGTGCGCTGGGCCATGCCGCCAGACAACTGCGCCGGGTACTGGCCACGGGCGTGGGCCAGGCCCACAGCCTCAATCGCATGGTCGATGCGTGCACGGCGTTCGGCATCCGCCAGCCTGGGCTGACgg                                                                                                                               <  2:716261/150‑1 (MQ=255)
                         tGCCAGGCAGCGGGCCAGCGCGGTGCGCTGGGCCATGCCGCCAGACAACTGCGCCGGGTACTGGCCACGGGCGTGGGCCAGGCCCACAGCCTCAATCGCATGGTCGATGCGTGCACGGCGTTCGGCATCCGCCAGCCt                                                                                                                                         >  2:418994/1‑138 (MQ=255)
                         tGCCAGGCAGCGGGCCAGCGCGGTGCGCTGGGCCATGCCGCCAGACAACTGCGCCGGGTACTGGCCACGGGCGTGGGCCAGGCCCACAGCCTCAATCGCATGGTCGATGCGTGCACGGCGTTCGGCATCCGCCAGCCt                                                                                                                                         <  1:418994/138‑1 (MQ=255)
                            cAGGCAGCGGGCCAGCGCGGTGCGCTGGGCCATGCCGCCAGACAACTGCGCCGGGTACTGGCCACGGGCGTGGGCCAGGCCCACAGCCTCAATCGCATGGTCGATGCGTGCACGGCGTTCGGCATCCGCCAGCCTGGGCTGACGGGCGaa                                                                                                                          >  2:729502/1‑150 (MQ=255)
                             aGGCAGCGGGCCAGCGCGGTGCGCTGGGCCATGCCGCCAGACAACTGCGCCGGGTACTGGCCACGGGCGTGGGCCAGGCCCACAGCCTCAATCGCATGGTCGATGCGTGCACGGCGTTCGTCATCCGCCAGCCTGGGCTGACGGGCGAAg                                                                                                                         >  1:98960/1‑150 (MQ=255)
                             aGGCAGCGGGCCAGCGCGGTGCGCTGGGCCATGCCGCCAGACAACTGCGCCGGGTACTGGCCACGGGCGTGGGCCAGGCCCACAGCCTCAATCGCATGGTCGATGCGTGCACGGCGTTCGTCATCCGCCAGCCTGGGCTGACGGGCGAAg                                                                                                                         >  1:99002/1‑150 (MQ=255)
                                  gCGGGCCAGCGCGGTGCGCTGGGCCATGCCGCCAGACAACTGCGCCGGGTACTGGCCACGGGCGTGGGCCAGGCCCACAGCCTCAATCGCATGGTCGATGCGTGCACGGCGTTCGGCATCCGCCAGCCTGGGCTGGCGGGCGAAGTCCAg                                                                                                                    <  1:584953/150‑1 (MQ=255)
                                    gggCCAGCGCGGTGCGCTGGGCCATGCCGCCAGACAACTGCGCCGGGTAATGGCCACGGGCGTGGGCCAGGCCCACAGCCTCAATCGCATGGTCGATGCGTGCACGGCGTTCGGCATCCGCCAGCCTGGGCTGACGGGCGAAGTCCAGGc                                                                                                                  <  2:1092089/150‑1 (MQ=255)
                                        cAGCGCGGTGCGCTGGGCCATGCCGCCAGACAACTGCGCCGGGTACTGGCCACGGGCGTGGGCCAGGCCCACAGCCTCAATCGCATGGTCGATGCGTGCACGGCGTTCGGCATCCGCCAGCCTGGGCTGACGGGCGAAGTCCAGGCCGaa                                                                                                              >  2:1132618/1‑150 (MQ=255)
                                                tGCGCTGGGCCATGCCGCCAGACAACTGCGCCGGGTACTGGCCACGGNCGTGGGCCAGGCCCACAGCCTCAATCGCATGGTCGATGCGTGCACGGCGTTCGGCATCCGCCAGCCTGGGCTGACGGGCGAAGTCCAGGCCGAAGGCGACGt                                                                                                      >  1:598119/1‑150 (MQ=255)
                                                         ccATGCCGCCAGACAACTGCGCCGGGTACTGGCCACGGGCGTGGGCCAGGCCCACAGCCTCAATCGCATGGTCGATGCGTGCACGGCGTTCGGCATCCGCCAGCCTGGGCTGACGGGCGAAGTCCAGGCCGAAGGCGACGTTTTTCTCCa                                                                                             >  2:1091596/1‑150 (MQ=255)
                                                          cATGCCGCCAGACAACTGCGCCGGGTACTGGCCACGGGCGTGGGCCAGGCCCACAGCCTCAATCGCATGGTCGATGCGTGCACGGCGTTCGGCATCCGCCAGCCTGGGCTGACGGGCGAAGTCCAGGCCGAAGGCGACGTTTTTCTCCAg                                                                                            >  2:1153334/1‑150 (MQ=255)
                                                          cATGCCGCCAGACAACTGCGCCGGGTACTGGCCACGGGCGTGGGCCAGGCCCACAGCCTCAATCGCATGGTCGATGCGTGCACGGCGTTCGGCATCCGCCAGCCTGGGCTGACGGGCGAAGTCCAGGCCGAAGGCGACGTTTTTCTCCAg                                                                                            <  1:729502/150‑1 (MQ=255)
                                                           aTGCCGCCAGACAACTGCGCCGGGTACTGGCCACGGGCGTGGGCCAGGCCCACAGCCTCAATCGCATGGTCGATGCGTGCACGGCGTTCAGCATCCGCCAGCCTGGGCTGACGGGCGAAGTCCAGGCCGAAGGCGACGTTTTTCTCCAgg                                                                                           <  2:203754/150‑1 (MQ=255)
                                                                gccAGACAACTGCGCCGGGTACTGGCCACGGGCGTGGGCCAGGCCCACAGCCTCAATCGCATGGTCGATGCGTGCACGGCGTTCGGCATCCGCCAGCCTGGGCTGACGGGCGAAGTCCAGGCCGAAGGCGACGTTTTTCTCCAGGCTCAg                                                                                      >  2:764211/1‑150 (MQ=255)
                                                                 ccAGACAACTGCGCCGGGTACTGGCCACGGGCGTGGGCCAGGCCCACAGCCTCAATCGCATGGTCGATGCGTGCACGGCGTTCGGCATCCGCCAGCCTGGGCTGACGGGCGAAGTCCAGGCCGAAGGCGACGTTTTTCTCCAGGCTCAGc                                                                                     >  2:544034/1‑150 (MQ=255)
                                                                  cAGACAACTGCGCCGGGTACTGGCCACGGGCGTGGGCCAGGCCCACAGCCTCAATCGCATGGTCGATGCGTGCACGGCGTTCGGCATCCGCCAGCCTGGGCTGACGGGCGAAGTCCAGGCCGAAGGCGACGTTTTTCTCCAGGCTCAGcc                                                                                    <  1:826140/150‑1 (MQ=255)
                                                                     aCAACTGCGCCGGGTACTGGCCACGGGCGTGGGCCAGGCCCACAGCCTCAATCGCATGGTCGATGCGTGCACGGCGTTCGTCATCCGCCAGCCTGGGCTGACGGGCGAAGTCCAGGCCGAAGGCGACGTTTTTCTCCAGGCTCAGCCATg                                                                                 <  2:99002/150‑1 (MQ=255)
                                                                     aCAACTGCGCCGGGTACTGGCCACGGGCGTGGGCCAGGCCCACAGCCTCAATCGCATGGTCGATGCGTGCACGGCGTTCGTCATCCGCCAGCCTGGGCTGACGGGCGAAGTCCAGGCCGAAGGCGACGTTTTTCTCCAGGCTCAGCCATg                                                                                 <  2:98960/150‑1 (MQ=255)
                                                                     aCAACTGCGCCGGGTACTGGCCACGGGCGTGGGCCAGGCCCACAGCCTCAATCGCATGGTCGATGCGTGCACGGCGTTCGGCATCCGCCAGCCTGGGCTGACGGGCGAAGTCCAGGCCGAAGGCGACGTTTTTCTCCAGGCTCAGCCATg                                                                                 <  2:713113/150‑1 (MQ=255)
                                                                      cAACTGCGCCGGGTACTGGCCACGGGCGTGGGCCAGGCCCACAGCCTCAATCGCATGGTCGATGCGTGCACGGCGTTCGGCATCCGCCAGCCTGGGCTGACGGGCGAAGTCCAGGCCGAAGGCGACGTTTTTCTCCAGGCTCAGCCATgg                                                                                <  1:1132618/150‑1 (MQ=255)
                                                                       aaCTGCGCCGGGTACTGGCCACGGGCGTGGGCCAGGCCCACAGCCTCAATCGCATGGTCGATGCGTGCACGGCGTTCGGCATCCGCCAGCCTGGGCTGACGGGCGAAGTCCAGGCCGAAGGCGACGTTTTTCTCCAGGCTCAGCCATGgc                                                                               >  1:65616/1‑150 (MQ=255)
                                                                              ccGGGTACTGGCCACGGGCGTGGGCCAGGCCCACAGCCTCAATCGCATGGTCGATGCGTGCACGGCGTTCGGCATCCGCCAGCCTGGGCTGACGGGCGAAGTCCAGGCCGAAGGCGACGTTTTTCTCCAGGCTCAGCCATGGCAGCAGGc                                                                        >  1:169708/1‑150 (MQ=255)
                                                                              ccGGGTACTGGCCACGGGCGTGGGCCAGGCCCACAGCCTCAATCGCATGGTCGATGCGTGCACGGCGTTCGGCATCCGCCAGCCTGGGCTGACGGGCGAAGTCCAGGCCGAAGGCGACGTTTTTCTCCAGGCTCAGCCATGGCAGCAGGc                                                                        >  1:846510/1‑150 (MQ=255)
                                                                                gggTACTGGCCACGGGCGTGGGCCAGGCCCACAGCCTCAATCGCATGGTCGATGCGTGCACGGCGTTCGGCATCCGCCAGCCTGGGCTGACGGGCGAAGTCCAGGCCGAAGGCGACGTTTTTCTCCAGGCTCAGCCATGGCAGCAGGCTg                                                                      <  1:1091596/150‑1 (MQ=255)
                                                                                     cTGGCCACGGGCGTGGGCCAGGCCCACAGCCTCAATCGCATGGTCGATGCGTGCACGGCGTTCGGCATCCGCCAGCCTGGGCTGACGGGCGAAGTCCAGGCCGAAGGCGACGTTTTTCTCCAGGCTCAGCCATGGCAGCAGGCTGGGATc                                                                 <  2:37007/150‑1 (MQ=255)
                                                                                          cACGGGCGTGGGCCAGGCCCACAGCCTCAATCGCATGGTCGATGCGTGCACGGCGTTCGGCATCCGCCAGCCTGGGCTGACGGGCGAAGTCCAGGCCGAAGGCGACGTTTTTCTCCAGGCTCAGCCATGGCAGCAGGCTGGGATCCTGaa                                                            >  1:262650/1‑150 (MQ=255)
                                                                                          cACGGGCGTGGGCCAGGCCCACAGCCTCAATCGCATGGTCGATGCGTGCACGGCGTTCGGCATCCGCCAGCCTGGGCTGACGGGCGAAGTCCAGGCCGAAGGCGACGTTTTTCTCCAGGCTCAGCCATGGCAGCAGGCTGGGATCCTGaa                                                            >  1:262918/1‑150 (MQ=255)
                                                                                              ggCGTGGGCCAGGCCCACAGCCTCAATCGCATGGTCGATGCGTGCACGGCGTTCGGCATCCGCCAGCCTGGGCTGACGGGCGAAGTCCAGGCCGAAGGCGACGTTTTTCTCCAGGCTCAGCCATGGCAGCAGGCTGGGATCCTGAAAAGc                                                        <  1:1160954/150‑1 (MQ=255)
                                                                                               gCGTGGGCCAGGCCCACAGCCTCAATCGCATGGTCGATGCGTGCACGGCGTTCGGCATCCGCCAGCCTGGGCTGACGGGCGAAGTCCAGGCCGAAGGCGACGTTTTTCTCCAGGCTCAGCCATGGCAGCAGGCTGGGATCCTGAAAAGcc                                                       <  1:88848/150‑1 (MQ=255)
                                                                                                      ccAGGCCCACAGCCTCAATCGCATGGTCGATGCGTGCACGGCGTTCGGCATCCGCCAGCCTGGGCTGACGGGCGAAGTCCAGGCCGAAGGCGACGTTTTTCTCCAGGCTCAGCCATGGCAGCAGGCTGGGATCCTGAAAAGCCACCGCCa                                                >  1:971003/1‑150 (MQ=255)
                                                                                                       cAGGCCCACAGCCTCAATCGCATGGTCGATGCGTGCACGGCGTTCGGCATCCGCCAGCCTGGGCTGACGGGCGAAGTCCAGGCCGAAGGCGACGTTTTTCTCCAGGCTCAGCCATGGCAGCAGGCTGGGATCCTGAAAAGCCACCGCCAg                                               >  2:1179833/1‑150 (MQ=255)
                                                                                                             cacaGCCTCAATCGCATGGTCGATGCGTGCACGGCGTTCGGCATCCGCCAGCCTGGGCTGACGGGCGAAGTCCAGGCCGAAGGCGACGTTTTTCTCCAGGCTCAGCCATGGCAGCAGGCTGGGATCCTGAAAAGCCAc                                                     >  2:252298/1‑138 (MQ=255)
                                                                                                             cacaGCCTCAATCGCATGGTCGATGCGTGCACGGCGTTCGGCATCCGCCAGCCTGGGCTGACGGGCGAAGTCCAGGCCGAAGGCGACGTTTTTCTCCAGGCTCAGCCATGGCAGCAGGCTGGGATCCTGAAAAGCCAc                                                     <  1:252298/138‑1 (MQ=255)
                                                                                                             cacaGCCTCAATCGCATGGTCGATGCGTGCACGGCGTTCGGCATCCGCCAGCCTGGGCTGACGGGCGAAGTCCAGGCCGAAGGCGACGTTTTTCTCCAGGCTCAGCCATGGCAGCAGGCTGGGATCCTGAAAAGCCACCGCCAGACGCgg                                         >  1:1016004/1‑150 (MQ=255)
                                                                                                                 gCCTCAATCGCATGGTCGATGCGTGCACGGCGTTCGGCATCCGCCAGCCTGGGCTGACGGGCGAAGTCCAGGCCGAAGGCGACGTTTTTCTCCAGGCTCAGCCATGGCAGCAGGCTGGGATCCTGAAAAGCCACCGCCAGACGCGGGTGc                                     <  2:65616/150‑1 (MQ=255)
                                                                                                                   cTCAATCGCATGGTCGATGCGTGCACGGCGTTCGGCATCCGCCAGCCTGGGCTGACGGGCGAAGTCCAGGCCGAAGGCGACGTTTTTCTCCAGGCTCAGCCATGGCAGCAGGCTGGGATCCTGAAAAGCCACCGCCAGACGCGGGTGCgg                                   >  1:174909/1‑150 (MQ=255)
                                                                                                                         cGCATGGTCGATGCGTGCACGGCGTTCGGCATCCGCCAGCCTGGGCTGACGGGCGAAGTCCAGGCCGAAGGCGACGTTTTTCTCCAGGCTCAGCCATGGCAGCAGGCTGGGATCCTGAAAAGCCACCGCCAGACGCGGGTGCGGCCCCTg                             >  2:605296/1‑150 (MQ=255)
                                                                                                                            aTGGTCGATGCGTGCACGGCGTTCGGCATCCGCCAGCCTGGGCTGACGGGCGAAGTCCAGGCCGAAGGCGACGTTTTtctc                                                                                               >  1:1011764/1‑81 (MQ=255)
                                                                                                                            aTGGTCGATGCGTGCACGGCGTTCGGCATCCGCCAGCCTGGGCTGACGGGCGAAGTCCAGGCCGAAGGCGACGTTTTtctc                                                                                               <  2:1011764/81‑1 (MQ=255)
                                                                                                                                 cGATGCGTGCACGGCGTTCGGCATCCGCCAGCCTGGGCTGACGGGCGAAGTCCAGGCCGAAGGCGACGTTTTTCTCCAGGCTCAGCCATGGCAGCAGGCTGGGATGCTTAAAAGCCACCGCCAGACGCGGGTGCGGACCATGCAGCGGCt                     <  2:840175/150‑1 (MQ=255)
                                                                                                                                  gATGCGTGCACGGCGTTCGGCATCCGCCAGCCTGGGCTGACGGGCGAAGTCCAGGCCGAAGGCGACGTTTTTCTCCAGGCTCAGCCATGGCAGCAGGCTGGGATCCTGAAAAGCCACCGCCAGACGCGGGTGCGGCCCCTGCAGCGGCTg                    <  1:544034/150‑1 (MQ=255)
                                                                                                                                       gTGCACGGCGTTCGGCATCCGCCAGCCTGGGCTGACGGGCGAAGTCCAGGCCGAAGGCGACGTTTTTCTCCAGGCTCAGCCATGGCAGCAGGCTGGGATCCTGAAAAGCCACCGCCAGACGCGGGTGCGGCCCCTGCAGTGGCTGGCCGt               <  2:298002/150‑1 (MQ=255)
                                                                                                                                           aCGGCGTTCGGCATCCGCCAGCCTGGGCTGACGGGCGAAGTCCAGGCCGAAGGCGACGTGTTTCTCCAGGCTCAGCCATGGCAGCAGGCTGGGATCCTGAAAAGCCACCGCCAGACGCGGGTGCGGCCCCTGCAGCGGCTGGCCGTGCaa           >  1:92673/1‑150 (MQ=255)
                                                                                                                                           aCGGCGTTCGGCATCCGCCAGCCTGGGCTGACGGGCGAAGTCCACGCCGAAGGCGACGTTTTTCTCCAGGCTCAGCCATGGCAGCAGGCTGGGATCCTGAAAAGCCACCGCCAGACGCGGGTGCGGCCCCTGCAGCGGCTGGCCGTGCaa           <  1:68238/150‑1 (MQ=255)
                                                                                                                                                    ggCATCCGCCAGCCTGGGCTGACGGGCGAAGTCCAGGCCGAAGGCGACGTTTTTCTCCAGGCTCAGCCATGGCAGCAGGCTGGGATCCTGAAAAGGGAAAGACTGACTCGGGTGCGGCCCCTGCAGCGGCTGGCCGTGCAAGCTCACGCt  >  2:937737/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
CCAGCAACAAGACTTCGGGCTGGCGTGCCAGGCAGCGGGCCAGCGCGGTGCGCTGGGCCATGCCGCCAGACAACTGCGCCGGGTACTGGCCACGGGCGTGGGCCAGGCCCACAGCCTCAATCGCATGGTCGATGCGTGCACGGCGTTCGGCATCCGCCAGCCTGGGCTGACGGGCGAAGTCCAGGCCGAAGGCGACGTTTTTCTCCAGGCTCAGCCATGGCAGCAGGCTGGGATCCTGAAAAGCCACCGCCAGACGCGGGTGCGGCCCCTGCAGCGGCTGGCCGTGCAAGCTCACGCT  >  NC_002947/4133523‑4133820

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑