Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,143,926 T→A 12.2% intergenic (+35/‑88) PP_3647 → / → PP_3648 oxidoreductase/carboxymuconolactone decarboxylase family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,143,9260TA12.2% 109.5 / 8.2 41intergenic (+35/‑88)PP_3647/PP_3648oxidoreductase/carboxymuconolactone decarboxylase family protein
Reads supporting (aligned to +/- strand):  ref base T (18/18);  new base A (2/3);  total (20/21)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.48e-01

CGGTGACCACGCGATCATTGTCGGCGCGGTGAAGAACCTGGTGTTGCCTGAGCAGCCGATGACCCCGATGGTGTACCACAAGGGCAAGCTCGGCGCCCTGCCGCCACTTGGCTGATACATCTTGAGTCGATGTTGGCATCTTCGCGGGCTTGCCCGCGAAGATGCCCCTGTTTTCAGGGGTCGCGCTAATCGTCTCACCCTCGGTTGCGGCCCCTTTTTTATGCCGGAGTACTGCACATGAGCAATGAAAAGTACGAAAAGGGCCTGAAAATCCGCACCCAGGT  >  NC_002947/4143777‑4144060
                                                                                                                                                     |                                                                                                                                      
cggTGACCACGCGATCATTGTCGGCGCGGTGAAGAACCTGGTGTTGCCTGAGCAGCCGATGACCCCGATGGTGTACCACAAGGGCAAGCTCGGCGCCCTGCCGCCACTTGGCTGATACATCTTGAGTCGATGTTGGCATCTTCGCGGGCt                                                                                                                                        >  2:105084/1‑150 (MQ=255)
       cACGCGATCATTGTCGGCGCGGTGAAGAACCTGGTGTTGCCTGAGCAGCCGATGACCCCGATGGTGTACCACAAGGGCAAGCTCGGCGCCCTGCCGCCACTTGGCTGATACATCTTGAGTCGATGTTGGCATCTTCGCGGGCAAGCCcgc                                                                                                                                 <  2:854927/150‑1 (MQ=255)
         cgcgATCATTGTCGGCGCGGTGAAGAACCTGGTGTTGCCTGAGCAGCCGATGACCCCGATGGTGTACCACAAGGGCAAGCTCGGCGCCCTGCCGCCACTTGGCTGATACATCTTGAGTCGATGTTGGCATCTTCGCGGGCTTGCCCGCGa                                                                                                                               <  1:567820/150‑1 (MQ=255)
                          gggTGAAGAACCTGGTGTTGCCTGAGCAGCCCATGACCCCGATGGTGTACCACAAGGGCAAGCTCGGCGCCCTGCCGCCACTTTGCTGATACATCTTGATTCGATGTTGGCATCTTGGCGGGCTTGCCCGCGAAGATGCCCCTGTTTTCa                                                                                                              <  2:43140/149‑1 (MQ=255)
                                 gaaCCTGGTGTTGCCTGAGCAGCCGATGACCCCGATGGTGTACCACAAGGGCAAGCTCGGCGCCCTGCCGCCACTTGGCTGATACATCTTGAGTCGATGTTGGCATCTTCGCGGGCTTGCCCGCGAAGATGCCCCTGTTTTCAGGGGTcg                                                                                                       <  1:424792/150‑1 (MQ=255)
                                     cTGGTGTTGCCTGAGCAGCCGATGACCCCGATGGTGTACCACAAGGGCAAGCTCGGCGCCCTGCCGCCACTTGGCTGATACATCTTGAGTCGATGTTGGCATCTTCGCGGGCTTGCCCGCGAAGATGCCCCTGTTTTCAg                                                                                                             <  2:751261/140‑1 (MQ=255)
                                     cTGGTGTTGCCTGAGCAGCCGATGACCCCGATGGTGTACCACAAGGGCAAGCTCGGCGCCCTGCCGCCACTTGGCTGATACATCTTGAGTCGATGTTGGCATCTTCGCGGGCTTGCCCGCGAAGATGCCCCTGTTTTCAg                                                                                                             >  1:751261/1‑140 (MQ=255)
                                               cTGAGCAGCCGATGACCCCGATGGTGTACCACAAGGGCAAGCTCGGCGCCCTGCCGCCACTTGGCTGATACATCTTGAGTCGATGTTGGCCTCTTCGCGGGCTTGCCCGCGAAGATGCCCCTGTTTTCAGGGGTCGCGCTAATCGTCTCa                                                                                         >  2:465659/1‑150 (MQ=255)
                                                  agcagcCGATGACCCCGATGGTGTACCACAAGGGCAAGCTCGGCGCCCTGCCGCCACTTGGCTGATACATCTTGAGTCGATGTTGGCATCTTCGCGGGCTTGCCCGCGAAGATGCCCCTGTTTTCAGGGGTCGCGCTAATCGTCTCAccc                                                                                      >  1:126788/1‑150 (MQ=255)
                                                    cagcCGATGACCCCGATGGTGTACCACAAGGGCAAGCTCGGCGCCCTGCCGCCACTTGGCTGATACATCTTGAGTCGATGTTGGCATCTTCGCGGGCTTGCCCGCGAAGATGCCCCTGTTTTCAGGGGTCGCGCTAATCGTCTCACCCTc                                                                                    >  1:1034825/1‑150 (MQ=255)
                                                     agcCGATGACCCCGATGGTGTACCACAAGGGCAAGCTCGGCGCCCTGCCGCCACTTGGCTGATACATCTTGAGTCGATGTTGGCATCTTCGCGGGCTTGCCCGCGAAGATGCCCCTGTTTTCAGGGGTCGCGCTAATCGTCTCACCCTCg                                                                                   >  1:282798/1‑150 (MQ=255)
                                                          aTGACCCCGATGGTGTACCACAAGGGCAAGCTCGGCGCCCTGCCGCCACTTGGCTGATACATCTTGAGTGGATGTTGGCATCTTCGCGGGCTTGCCCGCGAAGATGCCCCTGTTTTCAGGGGTCGCGCTAATCGTCTCACCCTCGGTTGc                                                                              <  2:126788/150‑1 (MQ=255)
                                                           tGACCCCGATGGTGTACCACAAGGGCAAGCTCGGCGCCCTGCCGCCACTTGGCTGATACATCTTGAGTCGATGTTGGCATCTTCGCGGGCTTGCCCGCGAAGATGCCCCTGTTTTCAGGGGTCGCGCTAATCGTCTCACCCTCGGTTGCg                                                                             <  1:966061/150‑1 (MQ=255)
                                                              ccccGATGGTGTACCACAAGGGCAAGCTCGGCGCCCTGCCGCCACTTGGCTGATACATCTTGAGTCGATGTTGGCATCTTCGCGGGCTTGCCCGCGAAGATGCCCCTGTTTTCAGGGGTCGCGCTAATCGTCTCACCCTCGGTTGCGGcc                                                                          <  1:89430/150‑1 (MQ=255)
                                                               cccGATGGTGTACCACAAGGGCAAGCTCGGCGCCCTGCCGCCACTTGGCTGATACATCTTGAGTCGATGTTGGCATCTTCGCGGGCTTGCCCGCGAAGATGCCCCTGTTTTCAGGGGTCGCGCTAATCGTCTCACCCTCGGTTGCGGccc                                                                         >  1:482982/1‑150 (MQ=255)
                                                                        gtACCACAAGGGCAAGCTCGGCGCCCTGCCTCCACTGGGCTGATACATCTTGAGTCGATGTTGGCATCTTCGCGGGCTTGCCCGCGAAGATGCCCCTGTTTTCAGGGGTCGCGCTAATCGTCTCACCCTCGGTTGCGGCCCCTTTTTTAt                                                                >  1:215056/1‑150 (MQ=255)
                                                                          aCCACAAGGGCAAGCTCGGCGCCCTGCCGCCACTTGGCTGATACATCTTGAGTCGATGTTGGCATCTTCGCGGGCTTGCCCGCGAAGATGCCCCTGTTTTCAGGGGTCGCGCTAATCGTCTCACCCTCGGTTGCGGCCCCTTTTTTATGc                                                              <  2:1034825/150‑1 (MQ=255)
                                                                                     aaGCTCGGCGCCCTGCCGCCACTTGGCTGATACATCTTGAGTCGATGTTGGCATCTTCGCGGGCTTGCCCGCGAAGATGCCCCTGTTTTCAGGGGTCGCGCTaa                                                                                                 <  1:658272/104‑1 (MQ=255)
                                                                                     aaGCTCGGCGCCCTGCCGCCACTTGGCTGATACATCTTGAGTCGATGTTGGCATCTTCGCGGGCTTGCCCGCGAAGATGCCCCTGTTTTCAGGGGTCGCGCTaa                                                                                                 >  2:658272/1‑104 (MQ=255)
                                                                                         tCGGCGCCCTGCCGCCACTTGGCTGATACATCTTGAGTCGATGTTGGCATCTTCGCGGGCTTGCCCGCGAAGATGCCCCTGTTTTCAGGGGTCGCGCTAATCGTCTCACCCTCGGTTGCGGCCCCTTTTTTATGCCGGAGTACTGCACAt                                               <  2:482982/150‑1 (MQ=255)
                                                                                         tCGGCGCCCTGCCGCCACTTGGCTGATACATCTTGAGTCGATGTTGGCATCTTCGCGGGCTTGCCCGCGAAGATGCCCCTGTTTTCAGGGGTCGCGCTAATCGTCTCACCCTCGGTTGCGGCCCCTTTTTTATGCCGGAGTACTGCACAt                                               <  2:265932/150‑1 (MQ=255)
                                                                                          cGGCGCCCTGCCGCCACTTGGCTGATACATCTTGAGTCGATGTTGGCATCTTCGCGGGCTTGCCCGCGAAGATGCCCCTGTTTTCAGGGGTCGCGCTAATCGTCTCACCCTCGGTTGCGGCCCCTTTTTTATGCCgg                                                           <  1:389013/137‑1 (MQ=255)
                                                                                          cGGCGCCCTGCCGCCACTTGGCTGATACATCTTGAGTCGATGTTGGCATCTTCGCGGGCTTGCCCGCGAAGATGCCCCTGTTTTCAGGGGTCGCGCTAATCGTCTCACCCTCGGTTGCGGCCCCTTTTTTATGCCgg                                                           >  2:389013/1‑137 (MQ=255)
                                                                                            gcgcCCTGCCGCCACTTGGCTGATACATCTTGAGTCGATGTTGGCATCTTCGCGGGCTTGCCCGCGAAGATGCCCCTGTTTTCAGGGGTCGCGCTAATCGTCTCACCCTCGGTTGCGGCCCCTTTTTTATGCCGGAGTACTGcac                                                 <  2:950964/145‑1 (MQ=255)
                                                                                            gcgcCCTGCCGCCACTTGGCTGATACATCTTGAGTCGATGTTGGCATCTTCGCGGGCTTGCCCGCGAAGATGCCCCTGTTTTCAGGGGTCGCGCTAATCGTCTCACCCTCGGTTGCGGCCCCTTTTTTATGCCGGAGTACTGcac                                                 >  1:950964/1‑145 (MQ=255)
                                                                                               cccTGCCGCCACTTGGCTGATACATCTTGAGTCGATGTTGGCATCTTCGCGGGCTTGCCCGCGAAGATGCCCCTGTTTTCAGGGGTCGCGCTAATCGTCTCACCCTCGGTTGCGGCCCCTTTTTTATGCCGGAGTACTGCACATGAGCaa                                         <  2:403368/150‑1 (MQ=255)
                                                                                                              gCTGATACATCTTGAGTCGATGGTGGCATCTTCGCGGGCTTGCCCGCGAAGATGCCCCTGTTTTCAGGGGTCGCGCTAATCGTCTCACCCTCGGTTGCGGCCCCTTTTTTATGCCGGAGTACTGCACATGAGCAATGAAAAGTACGaaaa                          <  1:999356/150‑1 (MQ=255)
                                                                                                                tGATACATCTTGAGTCGATGTTGGCATCTTCGCGGGCTTTCCCGCGAAGATGCCCCTGTTTTCAGGGGTCGCGCTAATCGTCTCACCCTCGGTTGCGGCCCCTTTTTTATGTCGGAGTACTGCACATGAGCAATGAAAAGTa                                <  1:304557/142‑1 (MQ=255)
                                                                                                                tGATACATCTTGAGTCGATGTTGGCATCTTCGCGGGCTTGCCCGCGAAGATGCCCCTGTTTTCAg                                                                                                             >  1:664635/1‑65 (MQ=255)
                                                                                                                tGATACATCTTGAGTCGATGTTGGCATCTTCGCGGGCTTGCCCGCGAAGATGCCCCTGTTTTCAg                                                                                                             <  2:664635/65‑1 (MQ=255)
                                                                                                                tGATACATCTTGAGTCGATGTTGGCATCTTCGCGGGCTTGCCCGCGAAGATGCCCCTGTTTTCAGGGGTCGCGCTAATCGTCTCACCCTCGGTTGCGGCCCCTTTTTTATGTCGGAGTACTGCACATGAGCAATGAAAAGTa                                >  2:304557/1‑142 (MQ=255)
                                                                                                                 gATACATCTTGAGTCGATGTTGGCATCTTCGCGGGCTTGCCCGCGAAGATGCCCCTGTTTTCAGGGGTCGCGCTAATCGTCTCACCCTCGGTTGCGGCCCCTTTTTTATGCCGGAGTACTGCACATGAGCAATGAAAAGTACGAAAAggg                       >  2:950061/1‑150 (MQ=255)
                                                                                                                         ttGAGTCGATGTTGGCATCTTCGCGGGCTTGCCCGCGAAGATGCCCCTGTTTTCAGGGGTCGCGCTAATCGTCTCACCCTCGGTTGCGGCCCCTTTTTTATGCCGGAGTACTGCACATGAGCAATGAAAAGTACGAAAAGGGCCTGaaaa               >  2:422361/1‑150 (MQ=255)
                                                                                                                            aGTCGATGTTGGCATCTTCGCGGGCTTGCCCGCGAAGATGCCCCTGTTTTCAGGGGTCGCGCTAATCGTCTCACCCTCGGTTGCGGCCCCTTTTTTATGCCGGAGTACTGCACATGAGCAATGAAAAGTACGAAAAGGGCCTGAAAATcc            >  2:743623/1‑150 (MQ=255)
                                                                                                                             gTCGATGTTGGCATCTTCGCGGGCTTGCCCGCGAAGATGCCCCTGTTTTCAGGGGTCGCGCTAATCGTCTCACCCTCGGTTGCGGCCCCTTTTTTATGCCGGAGTACTGCACATGAGCAATGGAAAGTACGAAAAGGGCCTGAAAATCCg           >  1:889/1‑150 (MQ=255)
                                                                                                                                      ggCATCTTCGCGGGCTTGCCCGCGAAGATGCCCCTGTTTTCAGGGGTCGCGCTAATCGTCTCACCCTCGGTTGCGGCCCCTTTTTTATGCCGGAGTACTGCACATGAGCAATGAAAAGTACGAAAAGGGCCTGAAAATCCGCACCCAGGt  >  1:1137732/1‑150 (MQ=255)
                                                                                                                                       gCATCTTCGCGGGCAAGCCCGCGAAGATGCCCCTGTTTTCAGGGGTCGCGCTAATCGTCTCACCCTCGGTTGCGGCCCCTTTTTTATGCCGGAGTACTGCACATGAGCAATGAAAAGTACGAAAAg                         <  1:1043707/126‑1 (MQ=255)
                                                                                                                                       gCATCTTCGCGGGCAAGCCCGCGAAGATGCCCCTGTTTTCAGGGGTCGCGCTAATCGTCTCACCCTCGGTTGCGGCCCCTTTTTTATGCCGGAGTACTGCACATGAGCAATGAAAAGTACGAAAAg                         >  2:1043707/1‑126 (MQ=255)
                                                                                                                                            ttCGCGGGCTTGCCCGCGAAGATGCCCCTGTTTTCAGGGGTCGCGCTAATCGTCTCACCCTCGGTTGCGGCCCCTTTTTTATGCCGGAGTACTGCACATGAGCAATGaaaa                                   >  1:1082854/1‑111 (MQ=255)
                                                                                                                                            ttCGCGGGCTTGCCCGCGAAGATGCCCCTGTTTTCAGGGGTCGCGCTAATCGTCTCACCCTCGGTTGCGGCCCCTTTTTTATGCCGGAGTACTGCACATGAGCAATGaaaa                                   <  2:1082854/111‑1 (MQ=255)
                                                                                                                                            ttCGCGGGCAAGCCCGCGAAGATGCCCCTGTTTTCAGGGGTCGCGCTAATCGTCTCACCCTCGGTTGCGGCCCCTTTTTTATGCCGGAGTACTGCACATGAGCAATGAAAAGTACGAAAAGGGcc                     >  1:835676/1‑125 (MQ=255)
                                                                                                                                            ttCGCGGGCAAGCCCGCGAAGATGCCCCTGTTTTCAGGGGTCGCGCTAATCGTCTCACCCTCGGTTGCGGCCCCTTTTTTATGCCGGAGTACTGCACATGAGCAATGAAAAGTACGAAAAGGGcc                     <  2:835676/125‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                      
CGGTGACCACGCGATCATTGTCGGCGCGGTGAAGAACCTGGTGTTGCCTGAGCAGCCGATGACCCCGATGGTGTACCACAAGGGCAAGCTCGGCGCCCTGCCGCCACTTGGCTGATACATCTTGAGTCGATGTTGGCATCTTCGCGGGCTTGCCCGCGAAGATGCCCCTGTTTTCAGGGGTCGCGCTAATCGTCTCACCCTCGGTTGCGGCCCCTTTTTTATGCCGGAGTACTGCACATGAGCAATGAAAAGTACGAAAAGGGCCTGAAAATCCGCACCCAGGT  >  NC_002947/4143777‑4144060

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑