Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,237,681 C→A 8.5% E205* (GAG→TAG) ‡ catB ← muconate cycloisomerase 1

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,237,6810CA 8.5% 149.3 / 2.2 47E205* (GAG→TAG) ‡catBmuconate cycloisomerase 1
Reads supporting (aligned to +/- strand):  ref base C (24/19);  new base A (2/2);  total (26/21)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.39e-01

TTCGATCGACTCATCGGCCATGATCGGAGCCGGACTGCGCTGGTTCAGGCGCACCTG‑G‑CCAGCGCGGTTGATGCGCGAAATCGGCTGCTCGATCAGGTCGATGCCGTTGTCGCCCAATACCTGGCAGGCGCGGATGGCCTGGGACTCGTCCCAGTACTGGTTGACGTCGACCCGCACGCTGGCGCTGTCACCCAGCTCGCGCTTGATCGCGACCACGTGCTTGAGGTCCTGCGCCACCGGGTTGGCGCCGATTTTCAGCTTGAACACGCGGTGCCGGCGAATGTCCAGCATGTGC  >  NC_002947/4237535‑4237829
                                                                                                                                                    |                                                                                                                                                    
ttCGATCGACTCATCGGCCATGATCGGAGCCGGACTGCGCTGGTTCAGGCGCACCTG‑G‑CCAGCGCGGTTGATGCGCGAAATCGGCTGCTCGATCAGGTCGATGCCGTTGTCGCCCAATACCTGGCAGGCGCGGATGGCCTGGGACTCGTc                                                                                                                                                   <  2:45345/150‑1 (MQ=255)
     tcgaCTCATCGGCCATGATCGGAGCCGGACTGCGCTGGTTCAGGCGCACCTG‑G‑CCAGCGCGGTTGATGCGCGAAATCGGCTGCTCGATCAGGTCGATGCCGTTGTCGCCCAATACCTGGCAGGCGCGGATGGCCTGGGACGAGTCCCAgg                                                                                                                                              <  2:690275/150‑2 (MQ=255)
            aTCGGCCATGATCGGAGCCGGACTGCGCTGGTTCAGGCGCACCTG‑G‑CCAGCGCGGTTGATGCGCGAAATCGGCTGCTCGATCAGGTCGATGCCGTTGTCGCCCAATACCTGGCAGGCGCGGATGGCCTGGGACTCGTCCCAGTACTgg                                                                                                                                         >  1:1017605/1‑148 (MQ=255)
            aTCGGCCATGATCGGAGCCGGACTGCGCTGGTTCAGGCGCACCTG‑G‑CCAGCGCGGTTGATGCGCGAAATCGGCTGCTCGATCAGGTCGATGCCGTTGTCGCCCAATACCTGGCAGGCGCGGATGGCCTGGGACTCGTCCCAGTACTgg                                                                                                                                         <  2:1017605/148‑1 (MQ=255)
                  cATGATCGGAGCCGGACTGCGCTGGTTCAGGCGCACCTG‑G‑CCAGCGCGGTTGATGCGCGAAATCGGCTGCTCGATCAGGTCGATGCCGTTGTCGCCCAATACCTGGCAGGCGCGGATGGCCTGGGACTCGTCCCAGTACTGGTTGACGTc                                                                                                                                 >  1:1087464/1‑150 (MQ=255)
                   aTGATCGGAGCCGGACTGCGCTGGTTCAGGCGCACCTG‑G‑CCAGCGCGGTTGATGCGCGAAATCGGCTGCTCGATCAGGTCGATGCCGTTGTCGCCCAATACCTGGCAGGCGCGGATGGCCTGGGACTCGTCCCAGTACTGGTTGACGTCg                                                                                                                                >  2:154151/1‑150 (MQ=255)
                         ggAGCCGGACTGCGCTGGTTCAGGCGCACCTG‑G‑CCAGCGCGGTTGATGCGCGAAATCGGCTGCTCGATCAGGTCGATGCCGTTGTCGCCCAATACCTGGCAGGCGCGGATGGCCTGGGACTCGTCCCAGTACTGGTTGACGTCGACCCGc                                                                                                                          <  1:36414/150‑1 (MQ=255)
                               caaccGCGCTGGC‑CAGGTGCGCCTGAA‑CCAGCGCGGTTGATGCGCGAAATCGGCTGCTCGATCAGGTCGATGCCGTTGTCGCCCAATACCTGGCAGGCGCGGATGGCCTGGGACTCGTCCCa                                                                                                                                                >  2:661837‑M1/6‑122 (MQ=255)
                               caaccGCGCTGGC‑CAGGTGCGCCTG‑AACCAGCGCGGTTGATGCGCGAAATCGGCTGCTCGATCAGGTCGATGCCGTTGTCGCCCAATACCTGGCAGGCGCGGATGGCCTGGGACTCGTCCCa                                                                                                                                                <  1:661837‑M1/117‑1 (MQ=255)
                                gACTGCGCTGGTTCAGGCGCACCTG‑G‑CCAGCGCGGTTGATGCGCGAAATCGGCTGCTCGATCAGGTCGATGCCGTTGTCGCCCAATACCTGGCAGGCGCGGATGGCCTGGGACTCGTCCCAGTACTGGTTGACGTCGACCCGCACGCTgg                                                                                                                   >  1:533747/1‑150 (MQ=255)
                                 aCTGCGCTGGTTCAGGCGCACCTG‑G‑CCAGCGCGGTTGATGCGCGAAATCGGCTGCTCGATCAGGTCGATGCCGTTGTCGCCCAATACCTGGCAGGCGCGGATGGCCTGGGACTCGTCCCAGTACTGGTTGACGTCGACCCGCACGCTGgc                                                                                                                  <  2:562043/150‑1 (MQ=255)
                                   tGCGCTGGTTCAGGCGCACCTG‑G‑CCAGCGCGGTTGATGCGCGAAATCGGCTGCTCGATCAGGTCGATGCCGTTGTCGCCCAATACCTGGCAGGCGCGGATGGCCTGGGACTCGTCCCAGTACTGGTTGACGTCGACCCGCACGCTGgcgc                                                                                                                >  1:35858/1‑150 (MQ=255)
                                                  gcACCTG‑G‑CCAGCGCGGTTGATGCGCGAAATCGGCTGCTCGATCAGGTCGATGCCGTTGTCGCCCAATACCTGGCAGGCGCGGATGGCCTGGGACTCGTCCCAGTACTGGTTGACGt                                                                                                                                  >  1:492305/1‑117 (MQ=255)
                                                  gcACCTG‑G‑CCAGCGCGGTTGATGCGCGAAATCGGCTGCTCGATCAGGTCGATGCCGTTGTCGCCCAATACCTGGCAGGCGCGGATGGCCTGGGACTCGTCCCAGTACTGGTTGACGt                                                                                                                                  <  2:492305/117‑1 (MQ=255)
                                                  gcACCTG‑G‑CCAGCGCGGTTGATGCGCGAAATCGGCTGCTCGATCAGGTCGATGCCGTTGTCGCCCAATACCTGGCAGGCGCGGATGGCCTGGGACTCGTCCCAGTACTGGTTGACGTCGACCCGCACGCTGGCGCTGTCa                                                                                                           >  1:224454/1‑140 (MQ=255)
                                                  gcACCTG‑G‑CCAGCGCGGTTGATGCGCGAAATCGGCTGCTCGATCAGGTCGATGCCGTTGTCGCCCAATACCTGGCAGGCGCGGATGGCCTGGGACTCGTCCCAGTACTGGTTGACGTCGACCCGCACGCTGGCGCTGTCa                                                                                                           <  2:224454/140‑1 (MQ=255)
                                                    aCCTG‑G‑CCAGCGCGGTTGATGCGCGAAATCGGCTGCTCGATCAGGTCGATGCCGTTGTCGCCCAATACCTGGCAGGCGCGGATGGCCTGGGACTCGTCCCAGTACTGGTTGACGTCGACCCGCACGCTGGCGCTGTCACCCAGCTCgcgc                                                                                               <  1:429068/150‑1 (MQ=255)
                                                     ccTG‑G‑CCAGCGCGGTTGATGCGCGAAATCGGCTGCTCGATCAGGTCGATGCCGTTGTCGCCCAATACCTGGCAGGCGCGGATGGCCTGGGACTCGTCCCAGTACTGGTTGACGTCGACCCGCACGCTGGCGCTGTCACCCAGCTCGCGCt                                                                                              >  1:227313/1‑150 (MQ=255)
                                                              agcgcGGTTGATGCGCGAAATCGGCTGCTCGATCAGGTCGATGCCGTTGTCGCCCAATACCTGGCAGGCGCGGATGGCCTGGGACTCGTCCCAGTACTGGTTGACGTCGACCCGCACGCTGGCGCTGTCACCCAGCTCGCGCTTGATcgc                                                                                       <  2:533747‑M2/145‑1 (MQ=255)
                                                                 gcGGTTGATGCGCGAAATCGGCTGCTCGATCAGGTCGATGCCGTTGTCGCCCAATACCTGGCAGGCGCGGATGGCCTGGGACTCGTCCCAGTACTGGTTGACGTCGACCCGCACGCTg                                                                                                                    <  2:161196‑M2/116‑1 (MQ=255)
                                                                 gcGGTTGATGCGCGAAATCGGCTGCTCGATCAGGTCGATGCCGTTGTCGCCCAATACCTGGCAGGCGCGGATGGCCTGGGACTCGTCCCAGTACTGGTTGACGTCGACCCGCACGCTg                                                                                                                    >  1:161196‑M2/3‑118 (MQ=255)
                                                                 gcGGTTGATGCGCGAAATCGGCTGCTCGATCAGGTCGATGCCGTTGTCGCCCAATACCTGGCAGGCGCGGATGGCCTGGGACTCGTCCCAGTACTGGTTGACGTCGACCCGCACGCTGGCGCTGTCACCCAGCTCGCGCTTGATCGCGAc                                                                                    <  1:154151‑M2/148‑1 (MQ=255)
                                                                    gTTGATGCGCGAAATCGGCTGCTCGATCAGGTCGATGCCGTTGTCGCCCAATACCTGGCAGGCGCGGATGGCCTGGGACTCGTCCCAGTAATGGTTGACGTTGACCCGCGCCCTCTCTCTGTCACCCAGCTCGCGCTTGATCGCGACCAc                                                                                 >  2:865083/1‑150 (MQ=255)
                                                                             cgAAATCGGCTGCTCGATCAGGTCGATGCCGTTGTCGCCCAATACCTGGCAGGCGCGGATGGCCTGGGACTCGTCCCAGTACTGGTTGACGTCGACCCGCACGCTGGCGCTGTCACCCAGCTCGCGCTTGATCGCGACCACGTGCTTGAg                                                                        <  2:227313/150‑1 (MQ=255)
                                                                              gAAATCGGCTGCTCGATCAGGTCGATGCCGTTGTCGCCCAATACCTGGCAGGCGCGGATGGCCTGGGACTCGTCCCAGTACTGGTTGACGTCGACCCGCACGCTGGCGCTGTCACCCAGCTCGCGCTTGATCGCGACCACGTGCTTGAgg                                                                       <  2:1087464/150‑1 (MQ=255)
                                                                                     gctgctCGATCAGGTCGATGCCGTTGTCGCCCAATACCTGGCAGGCGCGGATGGCCTGGGACGAGTCCCAgg                                                                                                                                              <  1:1047578/72‑2 (MQ=255)
                                                                                     gctgctCGATCAGGTCGATGCCGTTGTCGCCCAATACCTGGCAGGCGCGGATGGCCTGGGACGAGTCCCAgg                                                                                                                                              >  2:1047578/1‑71 (MQ=255)
                                                                                                       tGCCGTTGTCGCCCAATACCTGGCAGGCGCGGATGGCCTGGGACTCGTCCCAGTACTGGTTGACGTCGACCCGCACGCTGGCGCTGTCAcc                                                                                                         <  2:418981/91‑1 (MQ=255)
                                                                                                       tGCCGTTGTCGCCCAATACCTGGCAGGCGCGGATGGCCTGGGACTCGTCCCAGTACTGGTTGACGTCGACCCGCACGCTGGCGCTGTCAcc                                                                                                         >  1:418981/1‑91 (MQ=255)
                                                                                                          cGTTGTCGCCCAATACCTGGCAGGCGCGGATGGCCTGGGACTCGTCCCAGTACTGGTTGACGTCGACCCGCACGCTGGCGCTGTCACCCAGCTCGCGCTTGATCGCGACCACGTGCtt                                                                           <  2:679915/118‑1 (MQ=255)
                                                                                                          cGTTGTCGCCCAATACCTGGCAGGCGCGGATGGCCTGGGACTCGTCCCAGTACTGGTTGACGTCGACCCGCACGCTGGCGCTGTCACCCAGCTCGCGCTTGATCGCGACCACGTGCtt                                                                           >  1:679915/1‑118 (MQ=255)
                                                                                                            ttGTCGCCCAATACCTGGCAGGCGCGGATGGCCTGGGACTCGTCCCAGTACTGGTTGACGTCGACCCGCACGCTGGCGCTGTCACCCAGCTCGCGCTTGATCGCGACCACGTGCTTGAGGTCCTGCGCCACCGGGTTGGCGCCGATTTTc                                         >  2:722456/1‑150 (MQ=255)
                                                                                                               tCGCCCAATACCTGGCAGGCGCGGATGGCCTGGGACTCGTCCCAGTACTGGTTGACGTCGACCCGCACGCTGGCGCTGTCACCCAGCTCGCGCTTGATCGCGACCACGTGCTTGAGGTCCTGCGCCACCGGGTTGGCGCCGATTTTCAGc                                      <  2:35858/150‑1 (MQ=255)
                                                                                                               tCGCCCAATACCTGGCAGGCGCGGATGGCCTGGGACTCGTCCCAGTACTGGTTGACGTCGACCCGCACGCTGGCGCTGTCACCCAGCTCGCGCTTGATCGCGACCACGTGCTTGAGGTCCTGCGCCACCGGGTTGGCGCCGATTTTCAGc                                      >  1:365273/1‑150 (MQ=255)
                                                                                                                  cccAATACCTGGCAGGCGCGGATGGCCTGGGACTCGTCCCAGTACTGGTTGACGTCGACCCGCACGCTGGCGCTGTCACCCAGCTCGCGCTTGATCGCGACCACGTGCTTGAGGTCCTGCGCCACCGGGTTg                                                     >  1:885510/1‑132 (MQ=255)
                                                                                                                  cccAATACCTGGCAGGCGCGGATGGCCTGGGACTCGTCCCAGTACTGGTTGACGTCGACCCGCACGCTGGCGCTGTCACCCAGCTCGCGCTTGATCGCGACCACGTGCTTGAGGTCCTGCGCCACCGGGTTg                                                     <  2:885510/132‑1 (MQ=255)
                                                                                                                  cccAATACCTGGCAGGCGCGGATGGCCTGGGACTCGTCCCAGTACTGGTTGACGTCGACCCGCACGCTGGCGCTGTCACCCAGCTCGCGCTTGATCGCGACCACGTGCTTGAGGTCCTGCGCCACCGCGTTGGCGCCGATTTTCAGCTTg                                   >  2:122556/1‑150 (MQ=255)
                                                                                                                  cccAATACCTGGCAGGCGCGGATGGCCTGGGACTCGTCCCAGTACTGGTTGACGTCGACCCGCACGCTGGCGCCGCCACCCAGCTCGCGCTTGCTACCGCCGACGTGCGGGAGGTCCTGCGCCACCGGGTTGGCGCCGATTTTCAGCTTg                                   >  1:570700/1‑150 (MQ=255)
                                                                                                                         ccTGGCAGGCGCGGATGGCCTGGGACTCGTCCCAGTACTGGTTGACGTCGACCCGCACGCTGGCGCTGTCACCCAGCTCGCGCTTGATCGCGACCACGTGCTTGAGGTCCTGCGCCACCGGGTTGGCGCCGATTTTCAGCTTGAACAcgc                            <  1:865083/150‑1 (MQ=255)
                                                                                                                           tGGCAGGCGCGGATGGCCTGGGACTCGTCCCAGTACTGGTTGACGTCGACCCGCACGCTGGCGCTGTCACCCAGCTCGCGCTTGATCGCGACCACGTGCTTGAGGTCCTGCGCCACCGGGTTGGCGCCGATTTTCAGCTTGAACACGCgg                          >  1:344913/1‑150 (MQ=255)
                                                                                                                                  cgcgGATGGCCTGGGACTCGTCCCAGTACTGGTTGACGTCGACCCGCACGCTGGCGCTGTCACCCAGCTCGCGCTTGATCGCGACCACGTGCTTGAGGTCCTGCGCCACCGGGTTGGCGCCGATTTTCAGCTTGAACACGCGGTGCCGGc                   <  1:722456/150‑1 (MQ=255)
                                                                                                                                            cTGGGACTCGTCCCAGTACTGGTTGACGTCGACCCGCACGCTGGCGCTGTCACCCAGCTCGCGCTTGATCGCGACCACGTGCTTGAGGTCCTGCGCCACCGGGTTGGCGCCGATTTTCAGCTTGAACACGCGGTGCCGGCGAATGTCCAg         >  1:1027867/1‑150 (MQ=255)
                                                                                                                                            cTGGGACTCGTCCCAGTACTGGTTGACGTCGACCCGCACGCTGGCGCTGTCACCCAGCTCGCGCTTGATCGCGACCACGTGCTTGAGGTCCTGCGCCACCGGGTTGGCGCCGATTTTCAGCTTGAACACGCGGTGCCGGCGAATGTCCAg         >  1:205155/1‑150 (MQ=255)
                                                                                                                                            cTGGGACGAGTCCCAGTACTGGTTGACGTCGACCCGCACGCTGGCGCTGTCACCCAGCTCGCGCTTGATCGCGACCACGTGCTTGAGGTCCTGCGCCACCGGGTTGGCGCCGATTTTCAGCTTGAACACGCGGTGCCGGCGAATGTCCAg         >  2:240876/1‑150 (MQ=255)
                                                                                                                                               ggACTCGTCCCAGTACTGGTTGACGTCGACCCGCACGCTGGCGCTGTCACCCAGCTCGCGCTTGATCGCGACCACGTGCTTGAGGTCCTGCGCCACCGGGTTGGCGCCGATTTTCAGCTTGAACACGCGGTGCCGGCGAATGTCCAGCAt      >  1:1196194/1‑150 (MQ=255)
                                                                                                                                                  cTCGTCCCAGTACTGGTTGACGTCGACCCGCACGCTGGCGCTGTCACCCAGCTCGCGCTTGATCGCGACCACGTGCTTGAGGTCCTGCGCCACCGGGTTGGCGCCGATTTTCAGCTTGAACACGCGGTGCCGGCGAATGTCCAGCAtgtg   >  1:627691/1‑150 (MQ=255)
                                                                                                                                                   tCGTCCCAGTACTGGTTGACGTCGACCCGCACGCTGGCGCTGTCACCCAGCTCGCGCTTGATCGCGACCACGTGCTTGAGGTCCTGCGCCACCGGGTTGGCGCCGATTTTCAGCTTGAACACGCGGTGCCGGCGAATGTCCAGCAtgtgc  >  2:888335/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
TTCGATCGACTCATCGGCCATGATCGGAGCCGGACTGCGCTGGTTCAGGCGCACCTG‑G‑CCAGCGCGGTTGATGCGCGAAATCGGCTGCTCGATCAGGTCGATGCCGTTGTCGCCCAATACCTGGCAGGCGCGGATGGCCTGGGACTCGTCCCAGTACTGGTTGACGTCGACCCGCACGCTGGCGCTGTCACCCAGCTCGCGCTTGATCGCGACCACGTGCTTGAGGTCCTGCGCCACCGGGTTGGCGCCGATTTTCAGCTTGAACACGCGGTGCCGGCGAATGTCCAGCATGTGC  >  NC_002947/4237535‑4237829

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑