Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,248,656 A→C 7.7% L472R (CTG→CGG)  PP_3724 ← acyl‑CoA synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,248,6560AC 7.7% 127.6 / 2.5 47L472R (CTG→CGG) PP_3724acyl‑CoA synthetase
Reads supporting (aligned to +/- strand):  ref base A (25/18);  new base C (2/2);  total (27/20)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GGGGTAACCGACGCGGGCGGCGTCTGCCACGGCGCGTTCGGGCGTACTCAGCACGCCCGCAGGCACCGGCAGACCGAAGGCGCGCAGGGCCTGCTTGGATTGCCATTCGTCCAACAGTCGGCTTTCGCCCGTGAGGGCCTGCGGGCACAGCGGTACCCGCACAGCCTCGCCCTGGTCCATCAACCGCTGGCGGTTCTTTTGGTAAGCGACGATGCGCCCCCAGGCCGCCAGGCCGTCTTCCACACCCTGCAGGGCAGCGATGCCCCGCGCATGCAGGCGCTCACGCGCACTGGCA  >  NC_002947/4248508‑4248802
                                                                                                                                                    |                                                                                                                                                  
ggggTAACCGACGCGGGCGGCGTCTGCCACGGCGCGTTCGGGCGTACTCAGCACGCCCGCAGGCACCGGCAGACCGAAGGCGCGCAGGGCCTGCTTGGATTGCCATTCGTCCAACAGTCGGCTTTCGCCCGTGAGGGCCTGCGGGCACAg                                                                                                                                                   >  1:1200323/1‑150 (MQ=255)
                      tCTGCCACGGCGCGTTCGGGCGTACTCAGTACGCCCGCAGGCACCGGCAGACCGAAGGCGCGCAGGGCCTGCTTGGATTGCCATTCGTCCAACAGTCGGCTTTCGCCCGTGAGGGCCTGCGGGTACCGCTGTGCCCGcag                                                                                                                                       <  2:590607/140‑2 (MQ=255)
                      tCTGCCACGGCGCGTTCGGGCGTACTCAGTACGCCCGCAGGCACCGGCAGACCGAAGGCGCGCAGGGCCTGCTTGGATTGCCATTCGTCCAACAGTCGGCTTTCGCCCGTGAGGGCCTGCGGGTACCGCTGTGCCCGcag                                                                                                                                       >  1:590607/1‑139 (MQ=255)
                          ccACGGCGCGTTCGGGCGTACTCAGCACGCCCGCAGGCACCGGCAGACCGAAGGCGCGCAGGGCCTGCTTGGATTGCCATTCGTCCAACAGTCGGCTTTCGCCCGTGAGGGCCTGCGGGCACAGCGGTACCCGCACAGCCTCGCCCTGGt                                                                                                                         >  1:1043463/1‑150 (MQ=255)
                            aCGGCGCGTTCGGGCGTACTCAGCACGCCCGCAGGCACCGGCAGACCGAAGGCGCGCAGGGCCTGCTTGGATTGCCATTCGTCCAACAGTCGGCTTTCGCCCGTGAGGGCCTGCGGGCACAGCGGTACCCGCACAGCCTCGCCCTgg                                                                                                                          <  2:676334/147‑1 (MQ=255)
                            aCGGCGCGTTCGGGCGTACTCAGCACGCCCGCAGGCACCGGCAGACCGAAGGCGCGCAGGGCCTGCTTGGATTGCCATTCGTCCAACAGTCGGCTTTCGCCCGTGAGGGCCTGCGGGCACAGCGGTACCCGCACAGCCTCGCCCTgg                                                                                                                          >  1:676334/1‑147 (MQ=255)
                            aCGGCGCGTTCGGGCGTACTCAGCACGCCCGCAGGCACCGGCAGACCGAAGGCGCGCAGGGCCTGCTTGGATTGCCATTCGTCCAACAGTCGGCTTTCGCCCGTGAGGGCCTGCGGGCACAGCGGTACCCGCACAGCCTCGCCCTGGTc                                                                                                                        <  2:803379/149‑1 (MQ=255)
                            aCGGCGCGTTCGGGCGTACTCAGCACGCCCGCAGGCACCGGCAGACCGAAGGCGCGCAGGGCCTGCTTGGATTGCCATTCGTCCAACAGTCGGCTTTCGCCCGTGAGGGCCTGCGGGCACAGCGGTACCCGCACAGCCTCGCCCTGGTc                                                                                                                        >  1:803379/1‑149 (MQ=255)
                                       gggCGTACTCAGCACGCCCGCAGGCACCGGCAGACCGAAGGCGCGCAGGGCCTGCTTGGATTGCCATTCGTCCAACAGTCGGCTTTCGCCCGTGAGGGCCTGCGGGCACAGCGGTACCCGCACAGCCTCGCCCTGGTCCATCAACCGCTg                                                                                                            >  2:343140/1‑150 (MQ=255)
                                          cGTACTCAGCACGCCCGCAGGCACCGGCAGACCGAAGGCGCGCAGGGCCTGCTTGGATTGCCATTCGTCCAACAGTCGGCTTTCGCCCGTGAGGGCCTGCGGGCACAGCGGTACCCGCACAGCCTCGCCCTGGTCCATCAACCGCTGGCg                                                                                                         <  2:1130496/150‑1 (MQ=255)
                                             aCTCAGCACGCCCGCAGGCACCGGCAGACCGAAGGCGCGCAGGGCCTGCTTGGATTGCCATTCGTCCAACAGTCGGCTTTCGCCCGTGAGGGCCTGCGGGCACAGCGGTACCCGCACAGCCTCGCCCTGGTCCATCAACCGCTGGCGGtt                                                                                                      >  2:1005878/1‑150 (MQ=255)
                                               tCAGCACGCCCGCAGGCACCGGCAGACCGAAGGCGCGCAGGGCCTGCTTGGATTGCCATTCGTCCAACAGTCGGCTTTCGCCCGTGAGGGCCTGCGGGCACAGCGGTACCCGCACAGCCTCGCCCTGGTCCATCAACCGCTGGCGGTTCt                                                                                                    >  1:130192/1‑150 (MQ=255)
                                               tCAGCACGCCCGCAGGCACCGGCAGACCGAAGGCGCGCAGGGCCTGCTTGGATTGCCATTCGTCCAACAGTCGGCTTTCGCCCGTGAGGGCCTGCGGGCACAGCGGTACCCGCACAGCCTCGCCCTGGTCCATCAACCGCCAGCGGttga                                                                                                    <  2:989558/150‑3 (MQ=255)
                                                 aGCACGCCCGCAGGCACCGGCAGACCGAAGGCGCGCAGGGCCTGCTTGGATTGCCATTCGTCCAACAGTCGGCTTTCGCCCGTGAGGGCCTGCGGGCACAGCGGTACCCGCACAGCCTCGCCCTGGTCCATCAACCGCTGGCGGTTCttt                                                                                                  >  2:19413/1‑150 (MQ=255)
                                                      gCCCGCAGGCACCGGCAGACCGAAGGCGCGCAGGGCCTGCTTGGATTGCCATTCGTCCAACAGTCGGCTTTCGCCCGTGAGGGCCTGCGGGCACAGCGGTACCCGCACAGCCTCGCCCTGGTCCATCAACCGCTGGCGGTTCTTTTGGTa                                                                                             >  1:170195/1‑150 (MQ=255)
                                                          gCAGGCACCGGCAGACCGAAGGCGCGCAGGGCCTGCTTGGATTGCCATTCGTCCAACAGTCGGCTTTCGCCCGTGAGGGCCTGCGGGCACAACGGTACCCGCACAGCCTCGCCCTGGTCCATCAACCGCTGGCGGTTCTTTTGGTAAGcg                                                                                         >  2:961584/1‑150 (MQ=255)
                                                                aCCGGCAGACCGAAGGCGCGCAGGGCCTGCTTGGATTGCCATTCGTCCAACAGTCGGCTTTCGCCCGTGAGGGCCTGCGGGCACAGCGGTACCCGCACAGCCTCGCCCTGGTCCATCAACCGCTGGCGGTTCTTTTGGTAAGCGACGATg                                                                                   <  1:19413/150‑1 (MQ=255)
                                                                   ggCAGACCGAAGGCGCGCAGGGCCTGCTTGGATTGCCATTCGTCCAACAGTCGGCTTTCGCCCGTGAGGGCCTGCGGGCACAACGGTACCCGCACAGCCTCGCCCTGGTCCATCAACCGCTGGCGGTTCTTTTGGTAAGCGACGATgcgc                                                                                <  1:961584/150‑1 (MQ=255)
                                                                    gCAGACCGAAGGCGCGCAGGGCCTGCTTGGATTGCCATTCGTCCAACAGTCGGCTTTCGCCCGTGAGGGCCTGCGGGCACAGCGGTACCCGCACAGCCTCGCCCTGGTCCATCAACCGCTGGCGGTTCTTTTGGTAAGCGACGATGCGcc                                                                               >  2:120225/1‑150 (MQ=255)
                                                                       gACCGAAGGCGCGCAGGGCCTGCTTGGATTGCCATTCGTCCAACAGTCGGCTTTCGCCCGTGAGGGCCTGCGGGCACAGCGGTACCCGCACAGCCTCGCCCTGGTCCATCAACCGCTGGCGGTTCTTTTGGTAAGCGACGATGCGccccc                                                                            <  2:1178334/150‑1 (MQ=255)
                                                                         ccGAAGGCGCGCAGGGCCTGCTTGGATTGCCATTCGTCCAACAGTCGGCTTTCGCCCGTGAGGGCCTGCGGGCACAGCGGTACCCGCACAGCCTCGCCCTGGTCCATCAACCGCTGGCGGTTCTTTTGGTAAGCGACGATGCGCCCccag                                                                          >  2:402568/1‑150 (MQ=255)
                                                                         ccGAAGGCGCGCAGGGCCTGCTTGGATTGCCATTCGTCCAACAGTCGGCTTTCGCCCGTGAGGGCCTGCGGGCACAGCGGTACCCGCACAGCCTCGCCCTGGTCCATCAACCGCTGGCGGTTCTTTTGGTAAGCGACGATGCGCCCccag                                                                          <  2:1043463/150‑1 (MQ=255)
                                                                         ccGAAGGCGCGCAGGGCCTGCTTGGATTGCCATTCGTCCAACAGTCGGCTTTCGCCCGTGAGGGCCTGCGGGCACAGCGGTACCCGCACAGCCTCGCCCTGGTCCATCAACCGCTGGCGGTTCTTTTGGTAACCGTCGGTGCGCCCccag                                                                          <  2:1200323/150‑1 (MQ=255)
                                                                                       ggCCTGCTTGGATTGCCATTCGTCCAACAGTCGGCTTTCGCCCGTGAGGGCCTGCGGGCACAGCGGTACCCGCACAGCCTCGCCCTGGTCCATCAACCCCTTGCGGTTCTTTTGGTAAGCGACGATGCGCCCCCAGGCCGCCAGGCCGTc                                                            >  1:265494/1‑150 (MQ=255)
                                                                                          cTGCTTGGATTGCCATTCGTCCAACAGTCGGCTTTCGCCCGTGAGGGCCTGCGGGCACAGCGGTACCCGCACAGCCTCGCCCTGGTCCATCAACCGCTGGCGGTTCTTTTGGTAAGCGACGATGCGCCCCCAGGCCGCCAGGCCGTCTTc                                                         >  1:454888/1‑150 (MQ=255)
                                                                                                             tCCAACAGTCGGCTTTCGCCCGTGAGGGCCTGCGGGCACAGCGGTACCCGCACAGCCTCGCCCTGGTCCATCAACCGCTGGCGGTTCTTTTGGTAAGCGACGATGCGCCCCCAGGCCGCCAGGCCGTCTTCCACACCCTGCAGGGCAGCg                                      >  1:1078946/1‑150 (MQ=255)
                                                                                                                     tCGGCTTTCGCCCGTGAGGGCCTGCGGGCACAGCGGTACCCGCACAGCCTCGCCCTGGTCCATCAACCGCTGGCGGTTCttt                                                                                                  <  2:1189233/82‑1 (MQ=255)
                                                                                                                     tCGGCTTTCGCCCGTGAGGGCCTGCGGGCACAGCGGTACCCGCACAGCCTCGCCCTGGTCCATCAACCGCTGGCGGTTCTTTTGGTAAGCGACGATGCGCCCCCAGGCCGCCAGGCCGTCTTCCACAcc                                                   >  2:489433/1‑129 (MQ=255)
                                                                                                                     tCGGCTTTCGCCCGTGAGGGCCTGCGGGCACAGCGGTACCCGCACAGCCTCGCCCTGGTCCATCAACCGCTGGCGGTTCTTTTGGTAAGCGACGATGCGCCCCCAGGCCGCCAGGCCGTCTTCCACAcc                                                   <  1:489433/129‑1 (MQ=255)
                                                                                                                     tCGGCTTTCGCCCGTGAGGGCCTGCGGGCACAGCATTACCCGCACAGCCTCGCCCTGGTCCATCAACCGCTGGCGGTTCttt                                                                                                  >  1:1189233/1‑82 (MQ=255)
                                                                                                                       gccggtCGCCCGTGAGGGCCTGCGGGCACAGCGGTACCCGCACAGCCTCGCCCTGGTCCATCAACCGCTGGCGGTTCTTTTGGTAAGCGACGATGCGCCCCCAGGCCGCCAGGCCGTCTTCCACACCCTGCAGGGCAGCGATGCCCcgcg                            >  1:645261/6‑150 (MQ=255)
                                                                                                                        gCTTTCGCCCGTGAGGGCCTGCGGGCACAGCGGTACCCGCACAGCCTCGCCCTGGTCCATCAACCGCTGGCGGTTCTTTTg                                                                                                >  2:890581/1‑81 (MQ=255)
                                                                                                                        gCTTTCGCCCGTGAGGGCCTGCGGGCACAGCGGTACCCGCACAGCCTCGCCCTGGTCCATCAACCGCTGGCGGTTCTTTTg                                                                                                <  1:890581/81‑1 (MQ=255)
                                                                                                                          tttCGCCCGTGAGGGCCTGCGGGCACAGCGGTACCCGCACAGCCTCGCCCTGGTCCATCAACCGCTGGCGGTTCTTTTGGTAAGCGACGATGCGCCCCCAGGCCGCCAGGCCGTCTTCCACACCCTGCAGGGCAGCGATGCCCCGCGCAt                         >  2:143613/1‑150 (MQ=255)
                                                                                                                              gCCCGTGAGGGCGTGGGGGCACCGCGGTACCCGCACAGCCTCGCCCTGGTCCATCAACCGCTGGCGGTTCTTTTGGTAAGCGACGATGCGCCCCCAGGCCGCCAGGCCGTCTTCCACACCCTGCAGGGCAGCGATGCCCCGCGCATGCAg                     <  1:925282/150‑1 (MQ=255)
                                                                                                                               cccGTGAGGGCCTGCGGGCACAGCGGTACCCGCACAGCCTCGCCCTGGTCCATCAACCGCTGGCGGTTCTTTTGGTAAGCGACGATGCGCCCCCAGGCCGCCAGGCCGTCTTCCCCACCCTGCAGGGCAGCGATGCCCCGCGCATGCAgg                    >  2:284205/1‑150 (MQ=255)
                                                                                                                                ccGTGAGGGCCTGCGGGCACAGCGGTACCCGCACAGCCTCGCCCTGGTCCATCAACCGCTGGCGGTTCTTTTGGTAAGCGACGATGCGCCCCCAGGCCGCCAGGCCGTCTTCCACACCCTGCAGGGCAGCGATGCCCCGCGCATGCAGgc                   >  1:1043688/1‑150 (MQ=255)
                                                                                                                                ccGTGAGGGCCTGCGGGCACAGCGGTACCCGCACAGCCTCGCCCTGGTCCATCAACCGCTGGCGGTTCTTTTGGTAAGCGACGATGCGCCCCCAGGCCGCCAGGCCGTCTTCCACACCCTGCAGGGCAGCGATGCCCCGCGCATGCAGgc                   >  2:1018523/1‑150 (MQ=255)
                                                                                                                                  gTGAGGGCCTGCGGGCACAGCGGTACCCGCACAGCCTCGCCCTGGTCCATCAACCGCTGGCGGTTCTTTTGGTAAGCGACGATGCGCCCccaggcc                                                                       <  2:372191/96‑1 (MQ=255)
                                                                                                                                  gTGAGGGCCTGCGGGCACAGCGGTACCCGCACAGCCTCGCCCTGGTCCATCAACCGCTGGCGGTTCTTTTGGTAAGCGACGATGCGCCCccaggcc                                                                       >  1:372191/1‑96 (MQ=255)
                                                                                                                                  gTGAGGGCCTGCGGGCACAGCGGTACCCGCACAGCCTCGCCCTGGTCCATCAACCGCTGGCGGTTCTTTTGGTAAGCGACGATGCGCCCCCAGGCCGCCAGGCCGTCTTCCACACCCTGCAGGGCAGCGATGCCCCGCGCATGCAGgcgc                 <  2:645261/150‑1 (MQ=255)
                                                                                                                                      gggCCTGCGGGCACAGCGGTACCCGCACAGCCTCGCCCTGGTCCATCAACCGCTGGCGGTTCTTTTGGTAAGCGACGATGCGCCCCCAGGCCGCCAGGCCGTCTTCCACACCCTGCAGGGCAGCGATGCCCCGCGCATGc                       <  2:1108423/140‑1 (MQ=255)
                                                                                                                                      gggCCTGCGGGCACAGCGGTACCCGCACAGCCTCGCCCTGGTCCATCAACCGCTGGCGGTTCTTTTGGTAAGCGACGATGCGCCCCCAGGCCGCCAGGCCGTCTTCCACACCCTGCAGGGCAGCGATGCCCCGCGCATGc                       >  1:1108423/1‑140 (MQ=255)
                                                                                                                                        gCCTGCGGGCACAGCGGTACCCGCACAGCCTCGCCCTGGTCCATCAACCGCTGGCGGTTCTTTTGGTAAGCGACGATGCGCCCCCAGGCCGCCAGGCCGTCTTCCACACCCTGCAGGGCAGCGATGCCCCGCGCATGCAGGCGCTCAcgc           <  2:170195/150‑1 (MQ=255)
                                                                                                                                          tgtcGGGTACCGCTGTGCCCGCACAGCCTCGCCCTGGTCCATCAACCGCTGGCGGTTCTTTTGGTAAGCGACGATGCGCCCCCAGGCCGCCAGGCCGTCTTCCACACCCTGCAGGGCAGCGATGCCCCGCGCATGCAGGCGCTCgcgcgc         >  2:1108565/4‑150 (MQ=255)
                                                                                                                                           tGCGGGCACAGCGGTACCCGCACAGCCTCGCCCTGGTCCATCAACCGCTGGCGGTTCTTTTGGTAAGCGACGATGCGCCCCCAGGCCGCCAGGCCGTCTTCCACACCCTGCAGGGCAGCGATGCCCCGCGCATGCAGGCGCTCACGCGCa        <  2:139183/150‑1 (MQ=255)
                                                                                                                                              gggCACAGCGGTACCCGCACAGCCTCGCCCTGGTCCATCAACCGCTGGCGGTTCTTTTGGTAAGCGACGATGCGCCCCCAGGCCGCCAGGCCGTCTTCCACACCCTGCAGGGCAGCGATGCCCCGCGCATGCAGGCGCTCACGCGCACTg     <  2:454888/150‑1 (MQ=255)
                                                                                                                                                 cacaGCGGTACCCGCACAGCCTCGCCCTGGTCCATCAACCGCTGGCGGTTCTTTTGGTAAGCGACGATGCGCCCCCAGGCCGCCAGGCCGTCTTCCACACCCTGCAGGGCAGCGATGCCCCGCGCATGCAGGCGCTCACGCGCACTggca  <  2:1043688/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
GGGGTAACCGACGCGGGCGGCGTCTGCCACGGCGCGTTCGGGCGTACTCAGCACGCCCGCAGGCACCGGCAGACCGAAGGCGCGCAGGGCCTGCTTGGATTGCCATTCGTCCAACAGTCGGCTTTCGCCCGTGAGGGCCTGCGGGCACAGCGGTACCCGCACAGCCTCGCCCTGGTCCATCAACCGCTGGCGGTTCTTTTGGTAAGCGACGATGCGCCCCCAGGCCGCCAGGCCGTCTTCCACACCCTGCAGGGCAGCGATGCCCCGCGCATGCAGGCGCTCACGCGCACTGGCA  >  NC_002947/4248508‑4248802

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑