| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 4,296,911 | C→T | 8.3% | intergenic (+34/‑64) | gloA → / → PP_3767 | lactoylglutathione lyase/hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 4,296,911 | 0 | C | T | 8.3% | 169.3 / 4.6 | 60 | intergenic (+34/‑64) | gloA/PP_3767 | lactoylglutathione lyase/hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base C (26/29); new base T (2/3); total (28/32) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.53e-01 | |||||||||||
TGGAAGTGCCGTTCCAGAAACGCCTGCAGGACGGCCGCATGAACCACCTGGCCTTCGTCAAGGACCCGGACGGTTATTGGGTCGAAGTGATCCAGCCAACCGAGCTCGAAGGCTAAGCGTTCGTCGCGGCGCGCTCGTCAGGCCGGGAACTCCCGGCCTCTTGCTGTGGTATATGAAGGTAACTGCTTCACATACCACAGCGAGGTAAGGACGATGCAATCTCTTCACTGTGAATACCGCGAGCACACCATCACTGCCAGCGTGATGCCCCATCCCGACTCACCCCTGCCCTAT > NC_002947/4296762‑4297055 | tGGAAGTGCCGTTCCAGAAACGCCTGCAGGACGGCCGCATGAACCACCTGGCCTTCGTCAAGGACCCGGACGGTTATTGGGTCGAAGTGATCCAGCCAACCGAGCTCGAAGGCTAAGCGTTCGTCGCGGCGCGCTCGTCAGGCCGGGAAc > 1:563511/1‑150 (MQ=255) taaaGAAGCGCTTGCAGGACGGCCGCATGAACCACCTGGCCTTCGTCAAGGACCCGGACGGTTATTGGGTCGAAGTGATCCAGCCAACCGAGCTCGAAGGCTAAGCGTTCGTCGCGGCGCGCTCGTCAGGCCGGGAGTTCCCGGCctgac < 2:991292/147‑4 (MQ=255) aaCGCCTGCAGGACGGCCGCATGAACCACCTGGCCTTCGTCAAGGACCCGGACGGTTATTGGGTCGAAGTGATCCAGCCAACCGAGCTCGAAGGCTAAGCGTTCGTCGCGGCGCGCTCGTCAGGCCGGGAACTCCCGGCCTCTTGCtaga > 1:28250/1‑147 (MQ=255) aCGCCTGCAGGACGGCCGCATGAACCACCTGGCCTTCGTCAAGGACCCGGACGGTTATTGGGTCGAAGTGATCCAGCCAACCGAGCTCGAAGGCTAAGCGTTCGTCGCGGCGCGCTCGTCAGGCCGGGAACTCCCGGCCTCTTGCTGTgg > 2:1146478/1‑150 (MQ=255) ccTGCAGGACGGCCGCATGAACCACCTGGCCTTCGTCAAGGACCCGGACGGTTATTGGGTCGAAGTGATCCAGCCAACCGAGCTCGAAGGCTAAGCGTTCGTCGCGGCGCGCTCGTCAGGCCGGGAACTCCCGGCCTCTTGCTGTGGtat > 1:649665/1‑150 (MQ=255) cTGCAGGACGGCCGCATGAACCACCTGGCCTTCGTCAAGGACCCGGACGGTTATTGGGTCGAAGTGATCCAGCCAACCGAGCTCGAAGGCTAAGCGTTCGTCGCGGCGCGCTCGTCAGGCCGGGAACTCCCGGCCTCTTGCTGTGGtata < 1:1146478/150‑1 (MQ=255) aGGACGGCCGCATACACCACCTGGCTTTCGGCAAGGACACGGCCGATTGCAGCACGGGGCAGAGCCGAGCAACCGAGCTCGAAGGCTAAGCGTTCGTCGCGGCGCGCTCGTCAGGCCGGGAACTCCCGGCCTCTTGCTGTGGTATATGaa < 1:1047282/150‑1 (MQ=255) ggACGGCCGCATGAACCACCTGGCCTTCGTCAAGGACCCGGACGGTTATTGGGTCGAAGTGATCCAGCCAACCGAGCTCGAAGGCTAAGCGTTCGTCGCGGCGCGCTCGTCAGGCCGGGAACTCCCGGCCTCTTGCTGTGGTATATGAAg < 2:208607/150‑1 (MQ=255) ggACGGCCGCATGAACCACCTGGCCTTCGTCAAGGACCCGGACGGTTATTGGGTCGAAGTGATCCAGCCAACCGAGCTCGAAGGCTAAGCGTTCGTCGCGGCGCGCTCGTCAGGCCGGGAACTCCCGGCCTCTTGCTGTGGTATATGAAg < 2:208637/150‑1 (MQ=255) cGCATGAACCACCTGGCCTTCGTCAAGGACCCGGACGGTTATTGGGTCGAAGTGATCCAGCCAACCGAGCTCGAAGGCTAAGCGTTCGTCGCGGCGCGCTCGTCAGGCCGGGAACTCCCGGCCTCTTGCTGTGGTATATGAAGGTAACTg > 1:1089096/1‑150 (MQ=255) gCATGAACCACCTGGCCTTCGTCAAGGACCCGGACGGTTATTGGGTCGAAGTGATCCAGCCAACCGAGCTCGAAGGCTAAGCGTTCGTCGCGGCGCGCTCGTCAGGCCGGGAACTCCCGGCCTCTTGCTGTGGTATATGAAGGTAACTGc > 2:1151058/1‑150 (MQ=255) cATGAACCACCTGGCCTTCGTCAAGGACCCGGACGGTTATTGGGTCGAAGTGATCCCGAACACCGAGCTCAACGGCTAAGCTTGCGTCGCGGCGCGCTCGTCAGGCCGGGAACTCCCGGCCTCTTGCTGTGGTATATGAAGGTAACTGCt > 1:749220/1‑150 (MQ=255) accaccTGGCCTTCGTCAAGGACCCGGACGGTTATTGGGTCGAAGTGATCCAGCCAACCGAGCTCGAAGGCTAAGCGTTCGTCGCGGCGCGCTCGTCAGGCCGGGAACTCCCg < 2:673561/113‑1 (MQ=255) accaccTGGCCTTCGTCAAGGACCCGGACGGTTATTGGGTCGAAGTGATCCAGCCAACCGAGCTCGAAGGCTAAGCGTTCGTCGCGGCGCGCTCGTCAGGCCGGGAACTCCCg > 1:673561/1‑113 (MQ=255) accTGGCCTTCGTCAAGGACCCGGACGGTTATTGGGTCGAAGTGATCCAGCCAACCGAGCTCGAAGGCTAAGCGTTCGTCGCGGCGCGCTCGTCAGGCCGGGAACTCCCGGCCTCTTGCTGTGGTATATGAAGGTAACTGCTTCACATAc > 2:1051915/1‑150 (MQ=255) ggCCTTCGTCAAGGACCCGGACGGTTATTGGGTCGAAGTGATCCAGCCAACCGAGCTCGAAGGCTAAGCGTTCGTCGCGGCGCGCTCGTCAGGCCGGGAACTCCCGGCCTCTTGCtgtg > 2:568868/1‑119 (MQ=255) ggCCTTCGTCAAGGACCCGGACGGTTATTGGGTCGAAGTGATCCAGCCAACCGAGCTCGAAGGCTAAGCGTTCGTCGCGGCGCGCTCGTCAGGCCGGGAACTCCCGGCCTCTTGCtgtg < 1:568868/119‑1 (MQ=255) cTTCGTCAAGGACCCGGACGGTTATTGGGTCGAAGTGATCCAGCCAACCGAGCTCGAAGGCTAAGCGTTCGTCGCGGCGCGCTCGTCAGGCCGGGAACTCCCGGCCTCTTGCtgtg < 1:618525/116‑1 (MQ=255) cTTCGTCAAGGACCCGGACGGTTATTGGGTCGAAGTGATCCAGCCAACCGAGCTCGAAGGCTAAGCGTTCGTCGCGGCGCGCTCGTCAGGCCGGGAACTCCCGGCCTCTTGCtgtg > 2:618525/1‑116 (MQ=255) cTTCGTCAAGGACCCGGACGGTTATTGGGTCGAAGTGATCCAGCCAACCGAGCTCGAAGGCTAAGCGTTCGTCGCGGCGCGCTCGTCAGGCCGGGAACTCCCGGCCTCTTGCTGTGGTATATGAAGGTAACTGCTTCACATACCACAGCg > 1:445399/1‑150 (MQ=255) cTTCGTCAAGGACCCGGACGGTTATTGGGTCGAAGTGATCCAGCCAACCGAGCTCGAAGGCTAAGCGTTCGTCGCGGCGCGCTCGTCAGGCCGGGAACTCCCGGCCTCTTGCTGTGGTATATGAAGGTAACTGCTTCACATACCACAGCg > 1:689285/1‑150 (MQ=255) tCGTCAAGGACCCGGACGGTTATTGGGTCGAAGTGATCCAGCCAACCGAGCTCGAAGGCTAAGCGTTCGTCGCGGCGCGCTCGTCAGGCCGGGAACTCCCGGCCTCTTGCTGTGGTATATGAAGGTAACTGCTTCACATACCACAGCGAg < 1:156604/150‑1 (MQ=255) tCGTCAAGGACCCGGACGGTTATTGGGTCGAAGTGATCCAGCCAACCGAGCTCGAAGGCTAAGCGTTCGTCGCGGCGCGCTCGTCAGGCCGGGAACTCCCGGCCTCTTGCTGTGGTATATGAAGGTAACTGCTTCACATACCACAGCGAg < 1:679415/150‑1 (MQ=255) tCAAGGACCCGGACGGTTATTGGGTCGAAGTGATCCAGCCAACCGAGCTCGAAGGCTAAGCGTTCGTCGCGGCGCGCTCGTCAGGCCGGGAACTCCCGGCCTCTTGCTGTGGt < 1:1182431/113‑1 (MQ=255) tCAAGGACCCGGACGGTTATTGGGTCGAAGTGATCCAGCCAACCGAGCTCGAAGGCTAAGCGTTCGTCGCGGCGCGCTCGTCAGGCCGGGAACTCCCGGCCTCTTGCTGTGGt > 2:1182431/1‑113 (MQ=255) cctgACGGTTATTGGGTCGAAGTGATCCAGCCAACCGAGCTCGAAGGCTAAGCGTTCGTCGCGGCGCGCTCGTCAGGCCGGGAACTCCCGGCCTCTTGCTGTGGTATATGAAGGTAACTGCTTCACATACCACAGCGAGGTAAGGACGAt < 1:237730/147‑1 (MQ=255) ggACGGTTATTGGGTCGAAGTGATCCAGCCAACCGAGCTCGAAGGCTAAAAGGTCTTCGCGGCGCGCTCGTCAGGCCGGGAACTCCCGGCCTCTTGCTGTGGTATATGAAGGTAACTGCTTCACATACCACAGCGAGGTAAGGACGATGc > 1:85785/1‑150 (MQ=255) cGGTTATTGGGTCGAAGTGATCCAGCCAACCGAGCTCGAAGGCTAAGCGTTCGTCGCGGCGCGCTCGTCAGGCCGGGAACTCCCGGCCTCTTGCTGTGGTATATGAAGGTAACTGCTTCACATACCACAGCGAGGTAAGGACGATGCAAt < 2:445399/150‑1 (MQ=255) ttATTGGGTCGAAGTGATCCAGCCAACCGAGCTCGAAGGCTAAGCGTTCGTCGCGGCGCGCTCGTCAGGCCGGGAACTCCCGGCCTCTTGCTGTGGTATATGAAGGTAACTGCTTCACATACCACAGCGAGGTAAGGACGATGCAAtctc > 1:61822/1‑150 (MQ=255) tcactGGGTCGAAGTGAGCCAGCCAACCGAGCTCGAAGGCTAAGCGTTCGTCGCGGCGCGCTCGTCAGGCCGGGAACTCCCGGCCTCTTGCTGTGGTATATGAAGGTAACTGCTTCACATACCACAGCGAGGTAAGGACGATGCAAtctc < 2:85785/146‑1 (MQ=255) aTTGGGTCGAAGTGATCCAGCCAACCGAGCTCGAAGGCTAAGCGTTCGTCGCGGCGCGCTCGTCAGGCCGGGAACTCCCGGCCTCTTGCTGTGGTATATGAAGGTAACTGCTTCACATACCACAGCGAGGTAAGGACGATGCAATCTCtt < 2:61822/150‑1 (MQ=255) gggTCGAAGTGATCCAGCCAACCGAGCTCGAAGGCTAAGCGTTCGTCGCGGCGCGCTCGTCAGGCCGGGAACTCCCGGTCTCTTGCTGTgg < 1:793148/91‑1 (MQ=255) gggTCGAAGTGATCCAGCCAACCGAGCTCGAAGGCTAAGCGTTCGTCGCGGCGCGCTCGTCAGGCCGGGAACTCCCGGTCTCTTGCTGTgg > 2:793148/1‑91 (MQ=255) tCGAAGTGATCCAGCCAACCGAGCTCGAAGGCTAAGCGTTCGTCGCGGCGCGCTCGTCAGGCCGGGAACTCCCGGCCTCTTGCTGTGGTATATGAAGGTAACTGCTTCACATACCACAGCGAGGTAAGGACGATGAATTCTCTTAAcatg < 2:649665/150‑4 (MQ=255) aGTGATCCAGCCAACCGAGCTCGAAGGCTAAGCGTTCGTCGCGGCGCGCTCGTCAGGCCGGGAACTCCCGGCCTCTTGCtgt < 1:663265/82‑1 (MQ=255) aGTGATCCAGCCAACCGAGCTCGAAGGCTAAGCGTTCGTCGCGGCGCGCTCGTCAGGCCGGGAACTCCCGGCCTCTTGCtgt > 2:663265/1‑82 (MQ=255) aTCCAGCCAACCGAGCTCGAAGGCTAAGCGTTCGTCGCGGCGCGCTCGTCAGGCCGGGAACTCCCg > 1:718240/1‑66 (MQ=255) aTCCAGCCAACCGAGCTCGAAGGCTAAGCGTTCGTCGCGGCGCGCTCGTCAGGCCGGGAACTCCCg < 2:718240/66‑1 (MQ=255) tCCAGCCAACCGAGCTCGAAGGCTAAGCGTTCGTCGCGGCGCGCTCGTCAGGCCGGGAACTCCCGGCCTCTTGCTGTGGTATATGAAGGTAACTGCTTCACATACCACAGCGAGGTAAGGACGATGCAATCTCTTCACTGTGAATACcgc > 1:1046579/1‑150 (MQ=255) gAAGGCTAAGCGTTCGTCGCGGCGCGCTCGTCAGGCCGGGAACTCCCGGCCTCTTGCTGTGGTATATGAAGGTAACTGCTTCACATACCACAGCGAGGTAAGGACGATGCAATCTCTTCACTGTGAATACCGCGAGCACACCATCACTGc > 1:946565/1‑150 (MQ=255) aGGCTAAGCGTTCGTCGCGGCGCGCTCGTCAGGCCGGGGGTTCCCGGcc > 1:623618/1‑49 (MQ=255) aGGCTAAGCGTTCGTCGCGGCGCGCTCGTCAGGCCGGGGGTTCCCGGcc < 2:623618/49‑1 (MQ=255) aGGCTAAGCGTTCGTCGCGGCGCGCTCGTCAGGCCGGGAACTCCCGGCCTCTTGCTGTGGTATATGAAGGTAACTGCTTCACATACCACAGCGAGGTAAGGACGATGCAATCTCTTCACTGTGAATACCGCGAGCACACCATCACTGCCa < 2:1046579/150‑1 (MQ=255) gCTAAGCGTTCGTCGCGGCGCGCTCGTCAGGCCGGGAACTCCCGGCCTCTTGCTGTGGTATATGAAGGTAACTGCTTCACATACCACAGCGAGGTAAGGACGATGCAATCTCTTCACTGTGAATACCGCGAGCACACCATCACTGCCAGc > 1:114304/1‑150 (MQ=255) gCTAAGCGTTCGTCGCGGCGCGCTCGTCAGGCCGGGAACTCCCGGCCTCTTGCTGTGGTATATGAAGGTAACTGCTTCACATACCACAGCGAGGTAAGGACGATGCAATCTCTCCACTGTGAATACCGCGAGCACACCATCACTGCCAGc > 1:1191749/1‑150 (MQ=255) cTAAGCGTTCGTCGCGGCGCGCTCGTCAGGCCGGGAACTCCCGGCCTCTTGCTGTGGTATATGAAGGTAACTGCTTCACATACCACAGAGAGGTAAGGACGATGCAATCTCTTCACTGTGAATACCGCGAGCACACCATCACTGCCAGCg > 2:163507/1‑150 (MQ=255) tAAGCGTTCGTCGCGGCGCGCTCGTCAGGCCGGGAACTCCCGGCCTCTTGCTGTGGTATATGAAGGTAACTGCTTCACATACCACAGCGAGGTAAGGACGATGCAATCTCTTCACTGTGAATACCGCGAGCACACCATCACTGc < 1:475859/144‑1 (MQ=255) tAAGCGTTCGTCGCGGCGCGCTCGTCAGGCCGGGAACTCCCGGCCTCTTGCTGTGGTATATGAAGGTAACTGCTTCACATACCACAGCGAGGTAAGGACGATGCAATCTCTTCACTGTGAATACCGCGAGCACACCATCACTGc > 2:475859/1‑144 (MQ=255) aaGCGTTCGTCGCGGCGCGCTCGTCAGGCCGGGAACTCCCGGCCTCTTGCTGTGGTATATGAAGGTAACTGCTTCACATACCACAGCGAGGt > 2:962806/1‑92 (MQ=255) aaGCGTTCGTCGCGGCGCGCTCGTCAGGCCGGGAACTCCCGGCCTCTTGCTGTGGTATATGAAGGTAACTGCTTCACATACCACAGCGAGGt < 1:962806/92‑1 (MQ=255) aaGCGTTCGTCGCGGCGCGCTCGTCAGGCCGGGAACTCCCGGCCTCTTGCTGTGGTATATGAAGGTAACTGCTTCACATACCACAGCGAGGTAAGGACGATGCAATCTCTTCACTGTGAATACCGCGAGCACACCATCACTGCCAGCGTg < 2:1191749/150‑1 (MQ=255) gTTCGTCGCGGCGCGCTCGTCAGGCCGGGAACTCCCGGCCTCTTGCTGTGGTATATGAAGGTAACTGCTTCACATACCACAGCGAGGTAAGGACGATGCAATCTCTTCACTGTGAATACCGCGAGCACACCATCACTGc < 2:496941/139‑1 (MQ=255) gTTCGTCGCGGCGCGCTCGTCAGGCCGGGAACTCCCGGCCTCTTGCTGTGGTATATGAAGGTAACTGCTTCACATACCACAGCGAGGTAAGGACGATGCAATCTCTTCACTGTGAATACCGCGAGCACACCATCACTGc > 1:496941/1‑139 (MQ=255) ttCGTCGCGGCGCGCTCGTCAGGCCGGGAACTCCCGGCCTCTTGCTGTGGTATATGAAGGTAACTGCTTCACATACCACAGCGAGGTAAGGACGATGCAATCTCTTCACTGTGAATACCGCGAGCACACCATCACTGCCAGCGTGATGcc < 2:563511/150‑1 (MQ=255) ttCGTCGCGGCGCGCTCGTCAGGCCGGGAACTCCCGGCCTCTTGCTGTGGTATATGAAGGTAACTGCTTCACATACCACAGCGAGGTAAGGACGATGCAATCTCTTCACTGTGAATACCGCGAGCACACCATCACTGCCAGCGTGATGcc < 1:163507/150‑1 (MQ=255) gcggcgCGCTCGTCAGGCCGGGAACTCCCGGCCTCTTGCTGTGGTATATGAAGGTAACTGCTTCACATACCACAGCGAGGTAAGGACGATGCAATCTCTTCACTGTGAATACCGCGAGCACACCATCACTGCCAGCGTGATGCCCCATcc < 2:689285/150‑1 (MQ=255) cggcgCGCTCGTCAGGCCGTGAACTCCCGGCCTCTTGCTGTGGTATATGAAGGTAACTGCTTCACATACCACAGCGAGGTAAGGACGATGCAATCTCTTCACTGTGAATACCGCGAGCACACCATCACTGCCAGCGTGATGCCCCATccc < 1:517965/150‑1 (MQ=255) ggacgtgAACCCCCGGTCTCTTGCTGTGGTATATGAAGGTAACTGCTTCACATACCACAGCGAGGTAAGGACGATGCAATCTCTTCACTGTGAATACCGCGAGCACCCCATCACTGCCAGCGTGATGCCCCATCCCGACTCACCCCTGcc < 2:114304/144‑1 (MQ=255) ggCCGGGAGTTCCCGGCCTCTTGCTGTGGTATATGAAGGTAACTGCTTCACATACCACAGCGAGGTAAGGACGATGCAATCTCTTCACTGTGAATACCGCGa < 1:129373/102‑1 (MQ=255) ggCCGGGAGTTCCCGGCCTCTTGCTGTGGTATATGAAGGTAACTGCTTCACATACCACAGCGAGGTAAGGACGATGCAATCTCTTCACTGTGAATACCGCGa > 2:129373/1‑102 (MQ=255) gggAACTCCCGGCCTCTTGCTGTGGTATATGAAGGTAACTGCTTCACATACCACGGCGAGGTAAGGACGATGCAATCTCTTCACTGTGAATACCGCGAGCACACCATCACTGCCAGCGTGATGCCCCATCCCGACTCACCCCTGCCCTAt < 2:1089096/150‑1 (MQ=255) | TGGAAGTGCCGTTCCAGAAACGCCTGCAGGACGGCCGCATGAACCACCTGGCCTTCGTCAAGGACCCGGACGGTTATTGGGTCGAAGTGATCCAGCCAACCGAGCTCGAAGGCTAAGCGTTCGTCGCGGCGCGCTCGTCAGGCCGGGAACTCCCGGCCTCTTGCTGTGGTATATGAAGGTAACTGCTTCACATACCACAGCGAGGTAAGGACGATGCAATCTCTTCACTGTGAATACCGCGAGCACACCATCACTGCCAGCGTGATGCCCCATCCCGACTCACCCCTGCCCTAT > NC_002947/4296762‑4297055 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |