| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 4,297,638 | G→A | 6.5% | R154C (CGC→TGC) | PP_3768 ← | shikimate 5‑dehydrogenase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 4,297,638 | 0 | G | A | 6.5% | 180.8 / 2.1 | 61 | R154C (CGC→TGC) | PP_3768 | shikimate 5‑dehydrogenase |
| Reads supporting (aligned to +/- strand): ref base G (32/25); new base A (2/2); total (34/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.44e-01 | |||||||||||
GCCAGCTCTTCTGCCTCCAGCCCGCCTGCCATACCGATCGGCGTCACGTTCACCAGCATGGGCGGGCAAATGTCGCCTGGCTCAGGCACCCAGCGATAACCACAGACATCCGCCAACTGACGCCCGGCCTGCTCGTTGCGTGCAACGATGATGCCTTCGGCAAACCCGGCATCGCGCAAGGCGCTGGCCACGGCCTTGGCCATGCCACCGCTGCCTCGCAGGGCAAAGGCCGTGCCCGGATCGACCTGATGCTGAGCCAGCAATTGGCGCACGGCCAGGTAATCGGTGT > NC_002947/4297499‑4297787 | gCCAGCTCTTCTGCCTCCAGCCCGCCTGCCATACCGATCGGCGTCACGTTCACCAGCATGGGCGGGCAAATGTCGCCTGGCTCAGGCACCCAGCGATAACCACAGACATCCGCCAACTGACGCCCGGCCTGCTCGTTGCGTGCAACgatg > 1:67464/1‑150 (MQ=255) cAGCTCTCCTGTCTCCATGCCGCCTGCCATACCGATCGGCGTCACGTTCACCAGCATGGGCGGGCAAATGTCGCCTGGCTCAGGCACCCAGCGATAACCACAGACATCCGCCAACTGACGCCCGGCCTGCTCGTTGCGTGCAACgatgat < 2:974593/150‑1 (MQ=255) ttctGCCTCCAGCCCGCCTGCCATACCGATCGGCGTCACGTTCACCAGCAAACACGGGCAAATGTCGCCTGGCTCAGGCACCCAGCGATAACCACAGACATCCGCCAACTGACGCCCGGCCTGCTCGTTGCGTGCAACGATGATGCCTTc > 1:991221/1‑150 (MQ=255) cTCCAGCCCGCCTGCCATACCGATCGGCGTCACGTTCACCAGCATGGGCGGGCAAATGTCGCCTGGCTCAGGCACCCAGCGATAACCACAGACATCCGCCAACTGACGCCCGGCCTGCTCGTTGCGTGCAACGATGATGCCTTCGGCaaa > 2:988410/1‑150 (MQ=255) tCCAGCCCGCCTGCCATACCGATCGGCGTCACGTTCACCAGCATGGGCGGGCAAATGTCGCCTGGCTCAGGCACCCAGCGATAACCACAGACATCCGCCAACTGACGCCCGGCCTGCTCGTTGCGTGCAACGATGATGCCTTCGGCAAAc > 1:632678/1‑150 (MQ=255) tCCAGCCCGCCTGCCATACCGATCGGCGTCACGTTCACCAGCATGGGCGGGCAAATGTCGCCTGGCTCAGGCACCCAGCGATAACCACAGACATCCGCCAACTGACGCCCGGCCTGCTCGTTGCACGCAACgagca < 2:287580/136‑4 (MQ=255) tCCAGCCCGCCTGCCATACCGATCGGCGTCACGTTCACCAGCATGGGCGGGCAAATGTCGCCTGGCTCAGGCACCCAGCGATAACCACAGACATCCGCCAACTGACGCCCGGCCTGCTCGTTGCACGCAACgagca > 1:287580/1‑133 (MQ=255) tCCAGCCCGCCTGCCATACCGATCGGCGACAAGTTCAACAGGATGGGCGTGCAAATTGCGCCTGGCTCGGGCACTAGAAGACGTCCAGGCACACCCGCTAACTGACGCCCGGCCTGCTCGTTGCGTGCAACGCAGTATCCTTCGGCAAAc > 1:875889/1‑150 (MQ=255) gCCCGCCTGCCATACCGATCGGCGTCACGTTCACCAGCATGGGCGGGCAAATGTCGCCTGGCTCAGGCACCCAGCGATAACCACAGACATCCGCCAACTGACGCCCGGCCTGCTCGTTGCGTGCAACGATGATGCCTTCGGCAAACCCgg < 2:1047248/150‑1 (MQ=255) cccGCCTGCCATACCGATCGGCGTCACGTTCACCAGCATGGGCGGGCAAATGTCGCCTGGCTCAGGCACCCAGCGATAACCACAGACATCCGCCAACTGACGCCCGGCCTGCTCGTTGCGTGCAACGATGATGCCTTCGGCAAACCCGGc > 2:517325/1‑150 (MQ=255) cccGCCTGCCATACCGATCGGCGTCACGTTCACCAGCATGGGCGGGCAAATGTCGCCTGGCTCAGGCACCCAGCGATAACCACAGACATCCGCCAACTGACGCCCGGCCTGCTCGTTGCGTGCAACGATGATGCCTTCGGCAAACCCGGc > 1:613011/1‑150 (MQ=255) gCCATACCGATCGGCGTCACGTTCACCAGCATGGGCGGGCAAATGTCGCCTGGCTCAGGCACCCAGCGATAACCACAGACATCCGCCAACTGACGCCCGGCCTGCTCGTTGCACGCAACgag < 2:1050874/122‑2 (MQ=255) gCCATACCGATCGGCGTCACGTTCACCAGCATGGGCGGGCAAATGTCGCCTGGCTCAGGCACCCAGCGATAACCACAGACATCCGCCAACTGACGCCCGGCCTGCTCGTTGCACGCAACgag > 1:1050874/1‑121 (MQ=255) ccGATCGGCGTCACGTTCACCAGCATGGGCGGGCAAATGTCGCCTGGCTCAGGCACCCAGCGATAACCACAGACATCCGCCAACTGACGCCCGGCCTGCTCGTTGCGTGCAACGATGATGCCTTCGGCAAACCCGGCATCGCGCAAGgcg > 1:83013/1‑150 (MQ=255) gCGTCACGTTCACCAGCATGGGCGGGCAAATGTCGCCTGGCTCAGGCACCCAGCGATAACCACAGACATCCGCCAACTGACGCCCGGCCTGCTCGTTGCGTGCAACGATGATGCCTTCGGCAAACCCGGCATCGCGCAAGGCGCTGGCCa > 2:420125/1‑150 (MQ=255) tCACGTTCACCAGCATGGGCGGGCAAATGTCGCCTGGCTCAGGCACCCAGCGATAACCACAGACATCCGCCAACTGACGCCCGGCCTGCTCGTTGCGTGCAACGATGATGCCTTCGGCAAACCCGGCATCGCGCAAGGCGCTGGCCACgg < 2:875889/150‑1 (MQ=255) tCACGTTCACCAGCATGGGCGGGCAAATGTCGCCTGGCTCAGGCACCCAGCGATAACCACAGACATCCGCCAACTGACGCCCGGCCTGCTCGTTGCGTGCAACGATGATGCCTTCGGCAAACCCGGCATCGCGCAAGGCGCTGGCCACgg > 1:1059971/1‑150 (MQ=255) tCACCAGCATGGGCGGGCAAATGTCGCCTGGCTCAGGCACCCAGCGATAACCACAGACATCCGCCAACTGACGCCCGGCCTGCTCGTTGCGTGCAACGATGATGCCTTCGGCAAACCCGGCATCGCGCAAGGCGCTGGCCACGGCCTTgg < 2:1059971/150‑1 (MQ=255) cgcctGGGCGGGCAAATGTCGCCTGGCTCAGGCACCCAGCGATAACCACAGACATCCGCCAACTGACGCCCGGCCTGCTCGTTGCGTGCAACGATGATGCCTTCGGCAAACCCGGCATCGCGCAAGGCGCTGGCCAAGGCCGTGGCCagc < 2:991221/146‑3 (MQ=255) cATGGGCGGGCAAATGTCGCCTGGCTCAGGCACCCAGCGATAACCACAGACATCCGCCAACTGACGCCCGGCCTGCTCGTTGCGTGCAACGATGATGCCTTCGGCAAACCCGGCATCGCGCAGTGCGCTGGCCACCGCCTTGGCCATGcc > 2:454629/1‑150 (MQ=255) gTCGCCTGGCTCAGGCACCCAGCGATAACCACAGACATCCGCCAACTGACGCCCGGCCTGCTCGTTGCGTGCAACGATGATGCCTTCGGCAAACCCGGCATCGCGCAAGGCGCTGGCCACGGCCTTGGCCATGCCACCGCTGCCTCGCAg < 2:1200835/150‑1 (MQ=255) gTCGCCTGGCTCAGGCACCCAGCGATAACCACAGACATCCGCCAACTGACGCCCGGCCTGCTCGTTGCGTGCAACGATGATGCCTTCGGCAAACCCGGCATCGCGCAAGGCGCTGGCCACGGCCTTGGCCATGCCACCGCTGCCTCGCAg < 2:632678/150‑1 (MQ=255) tCGCCTGGCTCAGGCACCCAGCGATAACCACAGACATCCGCCAACTGACGCCCGGCCTGCTCGTTGCGTGCAACGATGATGCCTTCGGCAAACCCGGCATCGCGCAAGGCGCTGGCCACGGCCTTGGCCATGCCACCGCTGCCTCGCAgg > 1:601875/1‑150 (MQ=255) gCCTGGCTCAGGCACCCAGCGATAACCACAGACAACCGCCAACTGACGCCCGGCCTGCTCGTTGCGTGCAACGATGATGCCTTCGGCAAACCCGGCATCGCGCAAGGCGCTGGCCACGGCCTTGGCCATGCCACCGCTGCCTCGCAGGGc > 1:533247/1‑150 (MQ=255) gCTCAGGCACCCAGCGATAACCACAGACATCCGCCAACTGACGCCCGGCCTGCTCGTTGCGTGCAACGATGATGCCTTCGGCAAACCCGGCATCGCGCAAGGCGCTGGCCACGGCCTTGGCCATGCCACCGCTGCCTCGCAGGGCAAAgg > 1:184219/1‑150 (MQ=255) cTCAGGCACCCAGCGATAACCACAGACATCCGCCAACTGACGCCCGGCCTGCTCGTTGCGTGCAACGATGATGCCTTCGGCAAACCCGGCATCGCGCAAGGCGCTGGCCACGGCCTTGGCCATGCCACCGCTGCCTCGCAGGGCAAAGGc < 2:768303/150‑1 (MQ=255) ccAGCGATAACCACAGACATCCGCCAACTGACGCCCGGCCTGCTCGTTGCGTGTAACGATGATGCCTTCGGCAAACCCGGCATCGCGCAAGGCGCTGGCCACGGCCTTGGCCATGCCACCGCTGCCTCGCAGGGCAAAGGCCGTGCCCgg > 1:543142/1‑150 (MQ=255) cAGCGATAACCACAGACATCCGCCAACTGACGCCCGGCCTGCTCGTTGCGTGCAACGATGATGCCTTCGGCAAACCCGGCATCGCGCAAGGCGCTGGCCACGGCCTTGGCCATGCCACCGCTGCCTCGCAGGGCAAAGGCCGTGCCCGGa > 2:115544/1‑150 (MQ=255) aGCGATAACCACAGACATCCGCCAACTGACGCCCGGCCTGCTCGTTGCGTGCAACGATTATGCCTTCGGCAAACCCGGCATCGCGCAAGGCGCTGGCCACGGCCTTg < 1:667741/107‑1 (MQ=255) aGCGATAACCACAGACATCCGCCAACTGACGCCCGGCCTGCTCGTTGCGTGCAACGATTATGCCTTCGGCAAACCCGGCATCGCGCAAGGCGCTGGCCACGGCCTTg > 2:667741/1‑107 (MQ=255) gCGATAACCACAGACATCCGCCAACTGACGCCCGGCCTGCTCGTTGCGTGCAACGATGATGCCTTCg < 2:294219/67‑1 (MQ=255) gCGATAACCACAGACATCCGCCAACTGACGCCCGGCCTGCTCGTTGCGTGCAACGATGATGCCTTCg > 1:294219/1‑67 (MQ=255) gATAACCACAGACATCCGCCAACTGACGCCCGGCCTGCTCGTTGCGTGCAACGATGATGCCTTCGGCAAACCCGGCATCGCGCAAGGCGCTGGCCACGGCCTTGGCCATGCCACCGCTGCCTCGCAGGGCAAAGGCCGTGCCCGGATCGa > 2:664279/1‑150 (MQ=255) aTAACCACAGACATCCGCCAACTGACGCCCGGCCTGCTCGTTGCGTGCAACGATGATGCCTTCGGCAAACCCGGCATCGCGCAAGGCGCTGGCCACGGCCTTGGCCATGCCACCGCTGCCTCGCAGGGCAAAGGCCGTGCCCGGATCGAc < 1:805084/150‑1 (MQ=255) cacaGACATCCGCCAACTGACGCCCGGCCTGCTCGTTGCGTGCAACGATGATGCCTTCGGCAAACCCGGCATCGCGCAAGGCGCTGGCCACGGCCTTGGCCATGCCACCGCTGCCTCGCAGGGCAAAGGCCGTGCCCGGATCGACCTGAt < 1:988410/150‑1 (MQ=255) caGACATCCGCCAACTGACGCCCGGCCTGCTCGTTGCGTGCAACGATGATGCCTTCGGCAAACCCGGCATCGCGCAAGGCGCTGGCCACGGCCTTGGCCATGCCACCGCTGCCTCGCAGGGCAAAGGCCGTGCCCGGATCGACCTGATGc > 1:262639/1‑150 (MQ=255) aCATCCGCCAACTGACGCCCGGCCTGCTCGTTGCGTGCAACGATGATGCCTTCGGCAAACCCGGCATCGCGCAAGGCGCTGGCCACGGCCTTGGCCATGCCACCGCTGCCTCGCAGGGCAAAGGCCGTGCCCGGATCGACCTGATGCTGa < 2:67464/150‑1 (MQ=255) aCATCCGCCAACTGACGCCCGGCCTGCTCGTTGCGTGCAACGATGATGCCTTCGGCAAACCCGGCATCGCGCAAGGCGCTGGCCACGGCCTTGGCCATGCCACCGCTGCCTCGCAGGGCAAAGGCCGTGCCCGGATCGACCTGATGCTGa < 1:642069/150‑1 (MQ=255) aCATCCGCCAACTGACGCCCGGCCTGCTCGTTGCGTGCAACGATGATGCCTTCGGCAAACCCGGCATCGCGCAAGGCGCTGGCCACGGCCTTGGCCATGCCACCGCTGCCTCGCAGGGCAAAGGCCGTGCCCGGATCGACCTGATGCTGa < 2:613011/150‑1 (MQ=255) aCATCCGCCAACTGACGCCCGGCCTGCTCGTTGCGTGCAACGATGATGCCTTCGGCAAACCCGGCATCGCGCAAGGCGCTGGCCACGGCCTTGGCCATGCCACCGCTGCCTCGCAGGGCAAAGGCCGTGCCCGGATCGACCTGATGCTGa < 2:510701/150‑1 (MQ=255) aTCCGCCAACTGACGCCCGGCCTGCTCGTTGCGTGCAACGATGATGCCTTCGGCAAACCCGGCATCGCGCAAGGCGCTGGCCACGGCCTTGGCCATGCCACCGCTGCCTCGCAGGGCAAAGGCCGTGCCCGGATCGACCTGATGCTGAGc > 2:710750/1‑150 (MQ=255) tCCGCCAACTGACGCCCGGCCTGCTCGTTGCGTGCAACGATGATGCCTTCGGCAAACCCGGCATCGCGCAAGGCGCTGGCCACGGCCTTGGCCATGCCACCGCTGCCTCGCAGGGCAAAGGCCGTGCCCGGATCGACCTGATGCTGAGcc < 2:601875/150‑1 (MQ=255) cGCCAACTGACGCCCGGCCTGCTCGTTGCGTGCAACGATGATGCCTTCGGCAAACCCGGCATCGCGCAAGGCGCTGGCCACGGCCTTGGCCATGCCACCGCTGCCTCGCAGGGCAAAGGCCGTGCCCGGATCGACCTGATGCTGAGCCAg > 1:573202/1‑150 (MQ=255) gCCAACTGACGCCCGGCCTGCTCGTTGCGTGCAACGATGATGCCTTCGGCAAACCCGGCATCGCGCAAGGCGCTGGCCACGGCCTTGGCCATGCCACCGCTGCCTCGCAGGGCAAAGGCCGTGCCCGGATCGACCTGATGc > 2:1074969/1‑141 (MQ=255) gCCAACTGACGCCCGGCCTGCTCGTTGCGTGCAACGATGATGCCTTCGGCAAACCCGGCATCGCGCAAGGCGCTGGCCACGGCCTTGGCCATGCCACCGCTGCCTCGCAGGGCAAAGGCCGTGCCCGGATCGACCTGATGc < 1:1074969/141‑1 (MQ=255) ccAACTGACGCCCGGCCTGCTCGTTGCGTGCAACGATGATGCCTTCGGCAAACCCGGCATCGCGCAAGGCGCTGGCCACGGCCTTGGCCATGCCACCGCTGCCTCGCAGGGCAAAGGCCGTGCCCGGATCGACCTGATGCTGAGCCAGCa > 1:851270/1‑150 (MQ=255) cTGACGCCCGGCCTGCTCGTTGCGTGCAACGATGATGCCTTCGGCAAACCCGGCATCGCGCAAGGCGCTGGCCACGGCCTTGGCCATGCCACCGCTGCCTCGCAGGGCAAAGGCCGTGCCCGGATCGACCTGATGCTGAGCCAGCAATTg > 1:1037478/1‑150 (MQ=255) tGACGCCCGGCCTGCTCGTTGCGTGCAACGATGATGCCTTCGGCAAACCCGGCATCGCGCAAGGCGCTGGCCACGGCCTTGGCCATGCCACCGCTGCCTCGCAGGGCAAAGGCCGTGCCCGGATCGACCTGATGCTGAGCCAGCAATTgg < 2:1037478/150‑1 (MQ=255) gACGCCCGGCCTGCTCGTTGCGTGCAACGATGATGCCTTCGGCAAACCCGGCATCGCGCAAGGCGCTGGCCACGGCCTTGGCCATGCCACCGCTGCCTCGCAGGGCAAAGGCCGTGCCCGGATCGACCTGATGCTGAGCCAGCAATTGgc < 2:851270/150‑1 (MQ=255) cGCCCGGCCTGCTCGTTGCGTGCAACGATGATGCCTTCGGCAAACCCGGCATCGCGCAAGGCGCTGGCCACGGCCTTGGCCATGCCACCGCTGCCTCGCAGGGCAAAGGCCGTGCCCGGATCGACCTGATGCTGAGCCAGCAATTGgcgc > 1:315252/1‑150 (MQ=255) ccGGTCTGCTCGTTGCGTGCAACCATGATGCCTTCGGCAAACCCGGCATCGCGCAAGGCGCTGGCCACGGCCTTGGCCATGCCAACGCTGCCTCGCAGGGCAAAGGCCGTGCCCGGATCGACCTGATGCTGAGCCAGCAATTGGCGCACg < 1:421922/150‑1 (MQ=255) ccGGCCTGCTCGTTGCGTGCAACGATGATGCCTTCGGCAAACCCGGCATCGCGCAAGGCGCTGGCCACGGCCTTGGCCATGCCACCGCTGCCTCGCAGGGCAAAGGCCGTGCCCGGATCGACCTGATGCTGAGCCAGCAAt > 1:729360/1‑141 (MQ=255) ccGGCCTGCTCGTTGCGTGCAACGATGATGCCTTCGGCAAACCCGGCATCGCGCAAGGCGCTGGCCACGGCCTTGGCCATGCCACCGCTGCCTCGCAGGGCAAAGGCCGTGCCCGGATCGACCTGATGCTGAGCCAGCAAt < 2:729360/141‑1 (MQ=255) ccGGCCTGCTCGTTGCGTGCAACGATGATGCCTTCGGCAAACCCGGCATCGCGCAAGGCGCTGGCCACGGCCTTGGCCATGCCACCGCTGCCTCGCAGGGCAAAGGCCGTGCCCGGATCGACCTGATGCTGAGCCAGCAATTGGCGCACg > 1:996470/1‑150 (MQ=255) gCCTGCTCGTTGCGTGCAACGATGATGCCTTCGGCAAACCCGGCATCGCGCAAGGCGCTGGCCACGGCCTTGGCCATGCCACCGCTGCCTCGCAGGGCAAAGGCCGTGCCCGGATCGACCTGATGCTGAGCCAGCAATTGGCGCACGGcc > 1:477477/1‑150 (MQ=255) cTGCTCGTTGCGTGCAACGATGATGCCTTCGGCAAACCCGGCATCGCGCAAGGCGCTGGCCACGGCCTTGGCCATGCCACCGCTGCCTCGCAGGGCAAAGGCCGTGcc < 2:1014733/108‑1 (MQ=255) cTGCTCGTTGCGTGCAACGATGATGCCTTCGGCAAACCCGGCATCGCGCAAGGCGCTGGCCACGGCCTTGGCCATGCCACCGCTGCCTCGCAGGGCAAAGGCCGTGcc > 1:1014733/1‑108 (MQ=255) cTCGTTGCGTGCAACGATGATGCCTTCGGCAAACCCGGCATCGCGCAAGGCGCTGGCCACGGCCTTGGCCATGCCACCGCTGCCTCGCa > 2:394261/1‑89 (MQ=255) cTCGTTGCGTGCAACGATGATGCCTTCGGCAAACCCGGCACCGCGCAAGGCGCTGGCCACGGCCTTGGCCATGCCACCGCTGCCTCGCa < 1:394261/89‑1 (MQ=255) tCGTTGCGTGCAACGATGATGCCTTCGGCAAACCCGGCATCGCGCAAGGCGCTGGCCACGGCCTTGGCCATGCCACCGCTGCCTCGCAGGGCAAAGGCCGTGCCCGGATCGACCTGATGCTGAGCCAGCAATTGGCGCACGGCCAGGTaa > 2:788805/1‑150 (MQ=255) gCGTGCAACGATGATGCCTTCGGCAAACCCGGCATCGCGCAAGGCGCTGGCCACGGCCTTGGCCATGCCACCGCTGCCTCGCAGGGCAAAGGCCGTGCCCGGATCGACCTGATGCTGAGCCAGCAATTGGCGCACGGCCAGGTAATCGgt < 2:262639/150‑1 (MQ=255) gTGCAACGATGATGCCTTCGGCAAACCCGGCATCGCGCAAGGCGCTGGCCACGGCCTTGGCCATGCCACCGCTGCCTCGCAGGGCAAAGGCCGTGCCCGGATCGACCTGATGCTGAGCCAGCAATTGGCGCACGGCCAGGTAATCGgtgt > 2:75807/1‑150 (MQ=255) | GCCAGCTCTTCTGCCTCCAGCCCGCCTGCCATACCGATCGGCGTCACGTTCACCAGCATGGGCGGGCAAATGTCGCCTGGCTCAGGCACCCAGCGATAACCACAGACATCCGCCAACTGACGCCCGGCCTGCTCGTTGCGTGCAACGATGATGCCTTCGGCAAACCCGGCATCGCGCAAGGCGCTGGCCACGGCCTTGGCCATGCCACCGCTGCCTCGCAGGGCAAAGGCCGTGCCCGGATCGACCTGATGCTGAGCCAGCAATTGGCGCACGGCCAGGTAATCGGTGT > NC_002947/4297499‑4297787 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |