| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 4,338,437 | T→A | 7.5% | intergenic (+13/+129) | PP_3812 → / ← PP_5632 | lipase/allo‑isoleucine metabolism protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 4,338,437 | 0 | T | A | 7.5% | 160.3 / 4.3 | 53 | intergenic (+13/+129) | PP_3812/PP_5632 | lipase/allo‑isoleucine metabolism protein |
| Reads supporting (aligned to +/- strand): ref base T (24/25); new base A (2/2); total (26/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.52e-01 | |||||||||||
GCGTGACAGTAACCTCGGTGCGCTACAACGGAATGATTCATGACTATGGGCTGCTCAACCCGTTGAGCCAAGTGCCGGCAGTAAAGGCGGCGCTGCGGCAGGCGGGCAACGAACTTAAAGTGCATCTGCAG‑TGAGGCGATGAGCAATAGACCCGCCTGGGCGGGTCTTTTGCTTCTATTTGCGGAAACAGCCCGCAGGCTTCAATGAATCTGCCAGCGGTGCGGCAGGTCCGGCCCTTTCGCGGGTAAACCCGCGAAAGGGCCGGCAAATCCTGCTCAGGGCTCAAACCCTTC > NC_002947/4338291‑4338583 | gcgTGACAGTAACCTCGGTGCGCTACAACGGAATGATTCATGACTATGGGCTGCTCAACCCGTTGAGCCAAGTGCCGCAACTAAAGGCGGCGCTGCGGCAGGCGGGCAACGAACTTAAAGTGCATCTGCAG‑TGAGGCGATGAGCAATAGa > 2:528188/1‑150 (MQ=255) aGTAACCTCGGTGCGCTACAACGGAATGATTCATGACTATGGGCTGCTCAACCCGTTGAGCCAAGTGCCGGCAGTAAAGGCGGCGCTGCGGCAGGCGGGCAACGAACTTAAAGTGCATCTGCAG‑TGAGGCGATGAGCAATAGACCCGCCt > 1:676183/1‑150 (MQ=255) tAACCTCGGTGCGCTACAACGGAATGATTCATGACTATGGGCTGCTCAACCCGTTGAGCCAAGTGCCGGCAGTAAAGGCGGCGCTGCGGCAGGCGGGCAACGAACTTAAAGTGCATCTGCAG‑TGAGGCGATGAGCAATAGACCCGCCTgg > 2:449683/1‑150 (MQ=255) cTCGGTGCGCTACAACGGAATGATTCATGACTATGGGCTGCTCAACCCGTTGAGCCAAGTGCCGGCAGTAAAGGCGGCGCTGCGGCAGGCGGGCAACGAACTTAAAGTGCATCTGCAG‑TGAGGCGATGAGCAATAGACCCGCCTgg < 1:1084921/146‑1 (MQ=255) cTCGGTGCGCTACAACGGAATGATTCATGACTATGGGCTGCTCAACCCGTTGAGCCAAGTGCCGGCAGTAAAGGCGGCGCTGCGGCAGGCGGGCAACGAACTTAAAGTGCATCTGCAG‑TGAGGCGATGAGCAATAGACCCGCCTgg > 2:1084921/1‑146 (MQ=255) cTCGGTGCGCTACAACGGAATGATTCATGACTATGGGCTGCTCAACCCGTTGAGCCAAGTGCCGGCAGTAAAGGCGGCGCTGCGGCAGGCGGGCAACGAACTTAAAGTGCATCTGCAG‑TGAGGCGATGAGCAATAGACCCGCCTGGGCgg > 1:853257/1‑150 (MQ=255) cTCGGTGCGCTACAACGGAATGATTCATGACTATGGGCTGCTCAACCCGTTGAGCCAAGTGCCGGCAGTAAAGGCGGCGCTGCGGCAGGCGGGCAACGAACTTAAAGTGCATCTGCAG‑TGAGGCGATGAGCAATAGACCCGCCTGGGCgg < 2:676183/150‑1 (MQ=255) gcgcTACAACGGAATGATTCATGACTATGGGCTGCTCAACCCGTTGAGCCAAGTGCCGGCAGTAAAGGCGGCGCTGCGGCAGGCGGGCAACGAACTTAAAGTGCATCTGCAG‑TGAGGCGATGAGCAATAGACCCGCCTGGGCGGGTCttt < 2:364298/150‑1 (MQ=255) cgcTACAACGGAATGATTCATGACTATGGGCTGCTCAACCCGTTGAGCCAAGTGCCGGCAGTAAAGGCGGCGCTGCGGCAGGCGGGCAACGAACTTAAAGTGCATCTGCAG‑TGAGGCGATGAGCAAAAGACCCGCCCAGGCGGGTCtatt < 1:20597/150‑1 (MQ=255) gcTACAACGGAATGATTCATGACTATGGGCTGCTCAACCCGTTGAGCCAAGTGCCGGCAGTAAAGGCGGCGCTGCGGCAGGCGGGCAACGAACTTAAAGTGCATCTGCAG‑TGAGGCGATGAGCAATAGACCCGCCTGGGCGGGTCTTTTg < 2:522938/150‑1 (MQ=255) gcTACAACGGAATGATTCATGACCCTTGCCTGCTCAACCCGTTGAGCCAAGTGCCGGCAGTAAAGGCGGCGCTGCGGCAGGCGGGCAACGAACTTAAAGTGCATCTGCAG‑TGAGGCGATGAGCAATAGACCCGCCTGGGCGGGTCTTTTg < 1:604448/150‑1 (MQ=255) cAACGGAATGATTCATGACTATGGGCTGCTCAACCCGTTGAGCCAAGTGCCGGCAGTAAAGGCGGCGCTGCGGCAGGCGGGCAACGAACTTAAAGTGCATCTGCAG‑TGAGGCGATGAGCAATAGACCCGCCTGGGCGGGTCTTTTGCTTc < 2:780187/150‑1 (MQ=255) ggAATGATTCATGACTATGGGCTGCTCAACCCGTTGAGCCAAGTGCCGGCAGTAAAGGCGGCGCTGCGGCAGGCGGGCAACGAACTTAAAGTGCATCTGCAG‑TGAGGCGATGAGCAAAAGACCCGCCCAGGCGGGTCta > 1:748443/1‑138 (MQ=255) ggAATGATTCATGACTATGGGCTGCTCAACCCGTTGAGCCAAGTGCCGGCAGTAAAGGCGGCGCTGCGGCAGGCGGGCAACGAACTTAAAGTGCATCTGCAG‑TGAGGCGATGAGCAAAAGACCCGCCCAGGCGGGTCta < 2:748443/139‑2 (MQ=255) ggAAAGATTCCTCACTATAGGCTGCCCAACCCGTTAAGCCAAGTGCAGGCAGTAACGGCGGCGCTGCGGCAGGCGGCCAACGAACTTAAAGTGCATCTGCAC‑TCACGCGATGCGCCATAGACCCGCCTCCGCAGGTCTTTTGCTTCTCtt > 2:550322/1‑150 (MQ=255) aaTGATTCATGACTATGGGCTGCTCAACCCGTTGAGCCAAGTGCCGGCAGTAAAGGCGGCGCTGCGGCAGGCGGGCAACGAACTTAAAGTGCATCTGCAG‑TGAGGCGATGAGCAATAGACCCGCCTGGGCGGGTCTTTTGCTTCTATTTg > 2:788734/1‑150 (MQ=255) aaTGATTCATGACTATGGGCTGCTCAACCCGTTGAGCCAAGTGCCGGCAGTAAAGGCGGCGCTGCGGCAGGCGGGCAACGAACTTAAAGTGCATCTGCAG‑TGAGGCGATGAGCAATAGACCCGCCTGGGCGGGTCTTTTGCTTCTATTTg < 2:117812/150‑1 (MQ=255) tGATTCATGACTATGGGCTGCTCAACCCGTTGAGCCAAGTGCCGGCAGTAAAGGCGGCGCTGCGGCAGGCGGGCAACGAACTTAAAGTGCATCTGCAG‑TGAGGCGATGAGCAATAGACCCGCCTGGGCGGGTCTTTTGCTTCTATTTGCg < 2:813627/150‑1 (MQ=255) aTTCATGACTATGGGCTGCTCAACCCGTTGAGCCAAGTGCCGGCAGTAAAGGCGGCGCTGCGGCAGGCGGGCAACGAACTTAAAGTGCATCTGCAG‑TGAGGCGATGAGCAATa > 1:914587/1‑113 (MQ=255) aTTCATGACTATGGGCTGCTCAACCCGTTGAGCCAAGTGCCGGCAGTAAAGGCGGCGCTGCGGCAGGCGGGCAACGAACTTAAAGTGCATCTGCAG‑TGAGGCGATGAGCAATa < 2:914587/113‑1 (MQ=255) tCATGACTATGGGCTGCTCAACCCGTTGAGCCAAGTGCCGGCAGTAAAGGCGGCGCTGCGGCAGGCGGGCAACGAACTTAAAGTGCATCTGCAG‑TGAGGCGATGAGCAATAGACCAGCCTGGGCGGGTCTTTTGCTTCTATTTGCGGaaa > 2:275315/1‑150 (MQ=255) aTGACTATGGGCTGCTCAACCCGTTGAGCCAAGTGCCGGCAGTAAAGGCGGCGCTGCGGCAGGCGGGCAACGAACTTAAAGTGCATCTGCAG‑TGAGGCGATGAGCAATAGACCCGCCTGGGCGGGTCTTTTGCTTCTATTTGCGGAAACa > 2:1207655/1‑150 (MQ=255) tATGGGCTGCTCAACCCGTTGAGCCAAGTGCCGGCAGTAAAGGCGGCGCTGCGGCAGGCGGGCAACGAACTTAAAGTGCATCTGCAG‑TGAGGCGATGGGCAATAGACCCGCCTGGGCGGGTCTTTTGCTTCTATTTGCGGAAACAGCCCg > 1:746410/1‑150 (MQ=255) ctgctCAACCAGTTGAGCCAAGTGCCGGCAGTAAAGGCGGCGCTGCGGCAGGCGGGCAACGAACTTAAAGTGCATCTGCAG‑TGAGGCGATGAGCAATAGACCCGCCTGGGCGGGTCTTTTGCTTCTATTTGCGGAAACAGCCCGCAGGCt > 1:1154349/1‑150 (MQ=255) ctCAACCCGTTGAGCCAAGTGCCGGCAGTAAAGGCGGCGCTGCGGCAGGCGGGCAACGAACTTAAAGTGCATCTGCAG‑TGAGGCGATGAGCAATAGACCCGCCTGGGCGGGTCTTTTGCTTCTATTTGCGGAAACAGCCCGCAGGCTTCa > 2:598360/1‑150 (MQ=255) aCCCGTTGAGCCAAGTGCCGGCAGTAAAGGCGGCGCTGCGGCAGGCGGGCAACGAACTTAAAGTGCATCTGCAG‑TGAGGCGATGAGCAATAGACCCGCCTGGGc < 1:369205/104‑1 (MQ=255) aCCCGTTGAGCCAAGTGCCGGCAGTAAAGGCGGCGCTGCGGCAGGCGGGCAACGAACTTAAAGTGCATCTGCAG‑TGAGGCGATGAGCAATAGACCCGCCTGGGc > 2:369205/1‑104 (MQ=255) gAGCAAAGTGCCCGCAGTAAAGGCGGCGCTGCGGCAGGCGGGCAACCACCAAAAAGT‑CAACCGTTGCCCCTGCTTTGAGCAATAGACCCTCCTGGGCGGGTCTTTTGCTTCTATTTGCGGAAACAGCCCGCAGGCTTCAATGAATCTGcc < 1:275315/150‑1 (MQ=255) gCCAAGTGCCGGCAGTAAAGGCGGCGCTGCGGCAGGCGGGCAACGAACTTAAAGTGCATCTGCAG‑TGAGGCGATGAGCAATAGACCCGCCTGGGCGGGTCTTTTGCTTCTATTTGCGGAAACAGCCCGCAAGCTTCAATGAATCTGCCAg > 1:366672/1‑150 (MQ=255) ccAAGTGCCGGCAGTAAAGGCGGCGCTGCGGCAGGCGGGCAACGAACTTAAAGTGCATCTGCAG‑TGAGGCGATGAGCAATAGACCCGCCTGGGCGGGTCTTTTGCTTCTATTTGCg > 1:755102/1‑116 (MQ=255) ccAAGTGCCGGCAGTAAAGGCGGCGCTGCGGCAGGCGGGCAACGAACTTAAAGTGCATCTGCAG‑TGAGGCGATGAGCAATAGACCCGCCTGGGCGGGTCTTTTGCTTCTATTTGCg < 2:755102/116‑1 (MQ=255) aaGTGCCGGCAGTAAAGGCGGCGCTGCGGCAGGCGGGCAACGAACTTAAAGTGCATCTGCAG‑TGAGGCGATGAGCAATAGACCCGCCTGGGCGGGTCTTTTGCTTCTATTTGCGGAAACAGCCCGCAGGCTTCAATGAATCTGCCAGCgg > 1:946587/1‑150 (MQ=255) ccGGCAGTAAAGGCGGCGCTGCGGCAGGCGGGCAACGAACTTAAAGTGCATCTGCAG‑TGAGGCGATGAGCAATAGACCCGCCTGGGCGGGTCTTTTGCTTCTATTTGCGGAAACAGCCCGCAATTCTCAATGAATCTGCCAGCGGTGCgg < 1:766346/150‑1 (MQ=255) gTAAAGGCGGCGCTGCGGCAGGCGGGCAACGAACTTAAAGTGCATCTGCAG‑TGAGGCGATGAGCAATAGACCCGCCTGGGCGGGTCTTTTGCTTCTATTTGCGGAAACAGCCCGCAGGCTTCAATGAATCTGCCAGCGGTGCGGCAGGtt < 1:1207655/150‑2 (MQ=255) aaaGGCGGCGCTGCGGCAGGCGGGCAACGAACTTAAAGTGCATCTGCAG‑TGAGGCGATGAGCAATAGACCCGCCTGGGCGGGTCTTTTGCTTCTATTTGCGGAAACAGCCCGCAGGCTTCAATGAATCTGCCAGCGGTGCGGCAgg > 2:335316/1‑146 (MQ=255) aaaGGCGGCGCTGCGGCAGGCGGGCAACGAACTTAAAGTGCATCTGCAG‑TGAGGCGATGAGCAATAGACCCGCCTGGGCGGGTCTTTTGCTTCTATTTGCGGAAACAGCCCGCAGGCTTCAATGAATCTGCCAGCGGTGCGGCAgg < 1:335316/146‑1 (MQ=255) ggcgCTGCGGCAGGCGGGCAACGAACTTAAAGTGCATCTGCAG‑TGAGGCGATGAGCAATAGACCCGCCTGGGCGGGTCTTTTGCTTCTATTTGCGGAAACAGCCCGCAGGCTTCAATGAATCTGCCAGCgg > 1:707424/1‑131 (MQ=255) ggcgCTGCGGCAGGCGGGCAACGAACTTAAAGTGCATCTGCAG‑TGAGGCGATGAGCAATAGACCCGCCTGGGCGGGTCTTTTGCTTCTATTTGCGGAAACAGCCCGCAGGCTTCAATGAATCTGCCAGCgg < 2:707424/131‑1 (MQ=255) gcTGCGGCGGGCGGGCAACGAACTTAAAGTGCATCTGCAG‑TGAGGCGATGAGCAATAGACCCGCCTGGGCGGGTCTTTTGCTTCTATTTGCGGAAACAGCCCGCAGGCTTCAATGAATCTGCCAGCGGTGCGGCAGGTCCGGCCCTTTcg > 2:120892/1‑150 (MQ=255) cTGCGGCAGGCGGGCAACGAACTTAAAGTGCATCTGCAG‑TGAGGCGATGAGCAATAGACCCGCCTGGGCGGGTCTTTTGCTTCTATTTGCGGAAACAGCCCGCAGGCTTCAATGAATCTGCCAGCGGTGCGGCAGGTCCGGCCCTTTcgc < 2:1154349/150‑1 (MQ=255) gCGGCAGGCGGGCAACGAACTTAAAGTGCATCTGCAG‑TGAGGCGATGAGCAATAGACCCGCCTGGGCGGGTCTTTTGCTTCTATTTGCGGAAACAGCCCGCAGGCTTCAATGAATCTGCCAGCGGTGCGGCAGGTCCGGCCCTTTCGCgg < 2:853257/150‑1 (MQ=255) cGGCAGGCGGGCAACGAACTTAAAGTGCATCTGCAG‑TGAGGCGATGAGCAATAGACCCGCCTGGGCGGGTCTTTTGCTTCTATTTGCGGAAACAGCCCGCAGGCTTCAATGAATCTGCCAGCGGTGCGGCAGGTCCGGCCCTTTCGAggg > 1:1023438/1‑150 (MQ=255) ggCGGGCAACGAACTTAAAGTGCATCTGCAG‑TGAGGCGATGAGCAATAGACCCGCCTGGGCGGGTCTTTTGCTTCTATTTGCGGAAACAGCCCGCAGGCTGCAATGAATCTGCCAGCGGTGCGGCAGGTCCGGCCCTTTCGCGGGTAAAc > 1:379202/1‑150 (MQ=255) ccgtacaCGAACTTAAACTACCACCGAAG‑TTAGGCGATGAGCAATAGACCCGCCTGTGCGCGTCTTTTGTTTCTATTTGCGAAAACAGCCCGCCGGCTTCACCGAATCTGCCAGGGGTGCGGCAGGTCCGGCCCTTTCGCGGGTAAAccc < 2:396766/144‑1 (MQ=255) ggCAACGAACTTAAAGTGCATCTGCAG‑TGAGGCGATGAGCAATAGACCCGCCTGGGCGGGTCTTTTGCTTCTATTTGCGGAAACAGCCCGCAGGCTTCAATGAATCTGCCAGCGGTGCGGCAGGTCCGGCCCTTTCGCGGGTAAACCcgc < 1:120892/150‑1 (MQ=255) aCGAACTTAAAGTGCATCTGCAG‑TGAGGCGATGAGCAATAGACCCGCCTGGGCGTGTCTTTTGCTTCTATTTGCGGAAACAGCCCGCAGGCTTCAATGAATCTGCCAGCGGTGCGGCAGGTCCGGCCATTTCGCGGGTTTACCCGCGaaa < 1:528188/150‑1 (MQ=255) aCGAACTTAAAGTGCATCTGCAG‑TGAGGCGATGAGCAATAGACCCGCCTGGGCGGGTCTTTTGCTTCTATTTGCGGAAACAGCCCGCAGGCTTCAATGAATCTGCCAGCGGTGCGGCAGGTCCGGCCCTTTCGCGGGTTTACCCGCGaaa < 2:1023438/150‑1 (MQ=255) cGAACTTAAAGTGCATCTGCAG‑TGAGGCGATGAGCAATAGACCCGCCTGGGCGGGTCTTTTGCTTCTATTTGCGGAAACAGCCCGCAGGCTTCAATGAATCTGCCAGCGGTGCGGCAGGTCCGGCCCTTTCGCGGGTAAACCCGCGAAAg < 1:235673/150‑1 (MQ=255) cTTAAAGTGCATCTGCAG‑TGAGGCGATGAGCAATAGACCCGCCTGGGCGGGTCTTTTGCTTCTATTTGCGGAAACAGCCCGCAGGCTTCAATGAATCTGCCAGCGGTGCGGCAGGTCCGGCCCTTTCGCGGGTAAACCCGCGAAAGGGcc > 1:141215/1‑150 (MQ=255) tAAAGTGCATCTGCAG‑TGAGGCGATGAGCAATAGACCCGCCTGGGCGGGTCTTTTGCTTCTATTTGCGGAAACAGCCCGCAGGCTTCAATGAATCTGCCAGCGGTGCGGCAGGTCCGGCCCTTTCGCGGGTAAACCCGCGAAAGGGCCgg < 2:78227/150‑1 (MQ=255) gCATCTGCAG‑TGAGGCGATGAGCAATAGACCCGCCTGGGCGGGTCTTTTGCTTCTATTTGCGGAAACAGCCCGCAGGCTTCAATGAATCTGCCAGCGGTGCGGCAGGTCCGGCCCTTTCGCGGGTAAACCCGCGAAAGGGCCGGCAAATc < 2:379202/150‑1 (MQ=255) gCAAAAGACCCGCCCAGGCGGGTCTATTGCTTCTATTTGCGGAAACAGCCCGCAGGCTTCAATGAATCTGCCAGCGGTGCGGCAGGTCCGGCCCTTTCGCGGGTAAACCCGCGAAAGGGCCGGCAAATCCTGCTCAGGGCTCAAACCCtt > 2:20124/1‑150 (MQ=255) cAATAGACCCGCCTGGGCGGGTCTTTTGCTTCTATTTGCGGAAACAGCCCGCAGGCTTCAATGAATCTGCCAGCGGTGCGGCAGGTCCGGCCCTTTCGCGGGTAAACCCGCGAAAGGGCCGGCAAATCCTGCTCAGGGCTCAAACCCTTc > 1:1198373/1‑150 (MQ=255) | GCGTGACAGTAACCTCGGTGCGCTACAACGGAATGATTCATGACTATGGGCTGCTCAACCCGTTGAGCCAAGTGCCGGCAGTAAAGGCGGCGCTGCGGCAGGCGGGCAACGAACTTAAAGTGCATCTGCAG‑TGAGGCGATGAGCAATAGACCCGCCTGGGCGGGTCTTTTGCTTCTATTTGCGGAAACAGCCCGCAGGCTTCAATGAATCTGCCAGCGGTGCGGCAGGTCCGGCCCTTTCGCGGGTAAACCCGCGAAAGGGCCGGCAAATCCTGCTCAGGGCTCAAACCCTTC > NC_002947/4338291‑4338583 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |