Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	4,338,537	A→T	12.1%	intergenic (+113/+29)	PP_3812 → / ← PP_5632	lipase/allo‑isoleucine metabolism protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	4,338,537	0	A	T	12.1%	111.7 / 8.0	42	intergenic (+113/+29)	PP_3812/PP_5632	lipase/allo‑isoleucine metabolism protein
Reads supporting (aligned to +/- strand):  ref base A (16/20);  new base T (2/3);  total (18/24)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.18e-01

GGCGGGCAACGAACTTAAAGTGCATCTGCAGTGAGGCGATGAGCAATAGACCCGCCTGGGCGGGTCTTTTGCTTCTATTTGCGGAAACAGCCCGCAGGCTTCAATGAATCTGCCAGCGGTGCGGCAGGTCCGGCCCTTTCGCGGGTAAACCCGCGAAAGGGCCGGCAAATCCTGCTCAGGGCTCAAACCCTTCCACAATGATCACCTCAGCCTTCGCCACCCCTTGGCGATGGCTGCATGCCTCTTGGTACAGCGCGGAGCGGTAACAGGCGACGGCCTGCGCGTACGAATCGAA  >  NC_002947/4338391‑4338685                                                                                                                                                   |                                                                                                                                                     ggCGGGCAACGAACTTAAAGTGCATCTGCAGTGAGGCGATGAGCAATAGACCCGCCTGGGCGGGTCTTTTGCTTCTATTTGCGGAAACAGCCCGCAGGCTGCAATGAATCTGCCAGCGGTGCGGCAGGTCCGGCCCTTTCGCGGGTAAAc                                                                                                                                                   >  1:379202/1‑150 (MQ=255)   ccgtacaCGAACTTAAACTACCACCGAAGTTAGGCGATGAGCAATAGACCCGCCTGTGCGCGTCTTTTGTTTCTATTTGCGAAAACAGCCCGCCGGCTTCACCGAATCTGCCAGGGGTGCGGCAGGTCCGGCCCTTTCGCGGGTAAAccc                                                                                                                                                 <  2:396766/144‑1 (MQ=255)     ggCAACGAACTTAAAGTGCATCTGCAGTGAGGCGATGAGCAATAGACCCGCCTGGGCGGGTCTTTTGCTTCTATTTGCGGAAACAGCCCGCAGGCTTCAATGAATCTGCCAGCGGTGCGGCAGGTCCGGCCCTTTCGCGGGTAAACCcgc                                                                                                                                               <  1:120892/150‑1 (MQ=255)         aCGAACTTAAAGTGCATCTGCAGTGAGGCGATGAGCAATAGACCCGCCTGGGCGTGTCTTTTGCTTCTATTTGCGGAAACAGCCCGCAGGCTTCAATGAATCTGCCAGCGGTGCGGCAGGTCCGGCCATTTCGCGGGTTTACCCGCGaaa                                                                                                                                           <  1:528188/150‑1 (MQ=255)         aCGAACTTAAAGTGCATCTGCAGTGAGGCGATGAGCAATAGACCCGCCTGGGCGGGTCTTTTGCTTCTATTTGCGGAAACAGCCCGCAGGCTTCAATGAATCTGCCAGCGGTGCGGCAGGTCCGGCCCTTTCGCGGGTTTACCCGCGaaa                                                                                                                                           <  2:1023438/150‑1 (MQ=255)          cGAACTTAAAGTGCATCTGCAGTGAGGCGATGAGCAATAGACCCGCCTGGGCGGGTCTTTTGCTTCTATTTGCGGAAACAGCCCGCAGGCTTCAATGAATCTGCCAGCGGTGCGGCAGGTCCGGCCCTTTCGCGGGTAAACCCGCGAAAg                                                                                                                                          <  1:235673/150‑1 (MQ=255)              cTTAAAGTGCATCTGCAGTGAGGCGATGAGCAATAGACCCGCCTGGGCGGGTCTTTTGCTTCTATTTGCGGAAACAGCCCGCAGGCTTCAATGAATCTGCCAGCGGTGCGGCAGGTCCGGCCCTTTCGCGGGTAAACCCGCGAAAGGGcc                                                                                                                                      >  1:141215/1‑150 (MQ=255)                tAAAGTGCATCTGCAGTGAGGCGATGAGCAATAGACCCGCCTGGGCGGGTCTTTTGCTTCTATTTGCGGAAACAGCCCGCAGGCTTCAATGAATCTGCCAGCGGTGCGGCAGGTCCGGCCCTTTCGCGGGTAAACCCGCGAAAGGGCCgg                                                                                                                                    <  2:78227/150‑1 (MQ=255)                      gCATCTGCAGTGAGGCGATGAGCAATAGACCCGCCTGGGCGGGTCTTTTGCTTCTATTTGCGGAAACAGCCCGCAGGCTTCAATGAATCTGCCAGCGGTGCGGCAGGTCCGGCCCTTTCGCGGGTAAACCCGCGAAAGGGCCGGCAAATc                                                                                                                              <  2:379202/150‑1 (MQ=255)                                           gCAAAAGACCCGCCCAGGCGGGTCTATTGCTTCTATTTGCGGAAACAGCCCGCAGGCTTCAATGAATCTGCCAGCGGTGCGGCAGGTCCGGCCCTTTCGCGGGTAAACCCGCGAAAGGGCCGGCAAATCCTGCTCAGGGCTCAAACCCtt                                                                                                         >  2:20124/1‑150 (MQ=255)                                            cAATAGACCCGCCTGGGCGGGTCTTTTGCTTCTATTTGCGGAAACAGCCCGCAGGCTTCAATGAATCTGCCAGCGGTGCGGCAGGTCCGGCCCTTTCGCGGGTAAACCCGCGAAAGGGCCGGCAAATCCTGCTCAGGGCTCAAACCCTTc                                                                                                        >  1:1198373/1‑150 (MQ=255)                                                 gACCCGCCTGGGCGGGTCTTTTGCTTCTATTTGCGGAAACAGCCCGCAGGCTTCAATGAATCTGCCAGCGGTGCGGCAGGTCCGGCCCTTTCGCGGGTAAACCCGCGAAAGGGCCGGCAAATCCTGCTCAGGGCTCAAACCCTTCCACaa                                                                                                   <  2:946587/150‑1 (MQ=255)                                                    ccGCCTGGGCGGGTCTTTTGCTTCTATTTGCGGAAACAGCCCGCAGGCTTCAATGAATCTGCCAGCGGTGCGGCAGGTCCGGCCCTTTCGCGGGTTTACCCGCGAAAg                                                                                                                                          <  1:156404/108‑1 (MQ=255)                                                    ccGCCTGGGCGGGTCTTTTGCTTCTATTTGCGGAAACAGCCCGCAGGCTTCAATGAATCTGCCAGCGGTGCGGCAGGTCCGGCCCTTTCGCGGGTTTACCCGCGAAAg                                                                                                                                          >  2:156404/1‑108 (MQ=255)                                                    ccGCCTGGGCGGGTCTTTTGCTTCTATTTGCGGAAACAGCCCGCAGGCTTCAATGAATCTGCCAGCGGTGCGGCAGGTCCGGCCCTTTCGCGGGTAAACCCGCGAAAGGGCCGGCAAATCCTGCTCAGGGCTCAAACCCTTCCACAATGa                                                                                                >  1:667667/1‑150 (MQ=255)                                                    ccGCCTGGGCGGGTCTTTTGCTTCTATTTGCGGAAACAGCCCGCAGGCTTCAATGAATCTGCCAGCGGTGCGGCAGGTCCGGCCCTTTCGCGGGTAAACCCGCGAAAGGGCCGGCAAATCCTGCTCAGGGCTCAAACACTTCCAAAATGa                                                                                                >  2:1136657/1‑150 (MQ=255)                                                     cGCCTGGGCGGGTCTTTTGCTTCTATTTGCGGAAACAGCCCGCAGGCTTCAATGAATCTGCCAGCGGTGCGGCAGGTCCGGCCCTTTCGCGGGTAAACCCGCGAAAGGGCCGGCAAATCCTGCTCAGGGCTCAAACCCTTCCACAATGAt                                                                                               <  2:141215/150‑1 (MQ=255)                                                        cggggCGGGTTTTGTGCTTCTATTGGCGGAAACAGCCCGGAGGCTTCAATGGAGCTGCCAGCGGTGCGGCAGGTCCGGCCCTTTCGCGGGTAAACCCGCGAAAGGGCCGGCAAATCCTGCTCAGGGCTCAAACCCTTCCACAATGATCAc                                                                                            <  1:550322/148‑1 (MQ=255)                                                         tGGGCGGGTCTTTTGCTTCTATTTGCGGAAACAGCCCGCAGGCTTCAATGAATCTGCCAGCGGTGCGGCAGGTCCGGCCCTTTCGCGGGTAAACCCGCGAAAg                                                                                                                                          >  2:867430/1‑103 (MQ=255)                                                         tGGGCGGGTCTTTTGCTTCTATTTGCGGAAACAGCCCGCAGGCTTCAATGAATCTGCCAGCGGTGCGGCAGGTCCGGCCCTTTCGCGGGTAAACCCGCGAAAg                                                                                                                                          <  1:867430/103‑1 (MQ=255)                                                          gggCGGGTCTTTTGCTTCTATTTGCGGAAACAGCCCGCAGGCTTCAATGAATCTGCCAGCGGTGCGGCAGGTCCGGCCCTTTCGCGGGTAAACCCGCGAAAGGGCCGGCAAATCCTGCTCAGGGCTCAAACCCTTCCACAATGATCACCt                                                                                          >  1:38283/1‑150 (MQ=255)                                                                  cTTTTGCTTCTATTTGCGGAAACAGCCCGCAGGCTTCAATGAATCTGCCAGCGGTGCGGCAGGTCCGGCCCTTTCGCGGGTAAACCCGCGAAAGGGCCGGCAAATCCTGCTCAGGGCTCAAACCCTTCCACAATGATCACCTCAGCCTTc                                                                                  <  2:667667/150‑1 (MQ=255)                                                                         ttCTATTTGCGGAAACAGCCCGCAGGCTTCAATGAATCTGCCAGCGGTGCGGCAGGTCCGGCCCTTTCGCGGGTAAACCCGCGAAAGGGCCGGCAAATCCTGCTCAGGGCTCAAACCCTTCCACAATGATCACCTCAGCCTTCGCCAccc                                                                           <  2:1198373/150‑1 (MQ=255)                                                                                    gAAACAGCCCGCAGGCTTCAATGAATCTGCCAGCGGTGCGGCAGGTCCGGCCCTTTCGCGGGTAAACCCGCGAAAGGGCCGGCAAATCCTGCTCAGGGCTCAAACCCTTCCACAATGATCACCTCAGCCTTCGCCACCCCTTGGCGATgg                                                                <  1:20124/150‑1 (MQ=255)                                                                                      aaCAGCCCGCAGGCTTCAATGAATCTGCCAGCGGTGCGGCAGGTCCGGCCCTTTCGCGGGTAAACCCGCGAAAGGGCCGGCAAATcc                                                                                                                             >  2:947147/1‑87 (MQ=255)                                                                                      aaCAGCCCGCAGGCTTCAATGAATCTGCCAGCGGTGCGGCAGGTCCGGCCCTTTCGCGGGTAAACCCGCGAAAGGGCCGGCAAATcc                                                                                                                             <  1:947147/87‑1 (MQ=255)                                                                                        cAGCCCGCAGGCTTCAATGAATCTGCCAGCGGTGCGGCAGGTCCGGCCCTTTCGCGGGTAAACCCGCGAAAGGGCCGGCAAATCCTGCTCAggg                                                                                                                    >  2:1064097/1‑94 (MQ=255)                                                                                        cAGCCCGCAGGCTTCAATGAATCTGCCAGCGGTGCGGCAGGTCCGGCCCTTTCGCGGGTAAACCCGCGAAAGGGCCGGCAAATCCTGCTCAggg                                                                                                                    <  1:1064097/94‑1 (MQ=255)                                                                                        cAGCCCGCAGGCTTCAATGAATCT‑CCAGCGGTGCGGCAGGTCCGGCCCTTTCGCGGGTAAACCCGCGAAAGGGCCGGCAAATCCTGCTCAGGGCTCAAACCCTTCCACAATGATCACCTCAGCCTTCGCCACCCCTTGGCGATGGCTGCa                                                           <  1:598360/150‑1 (MQ=255)                                                                                         aGCCCGCAGGCTTCAATGAATCTGCCAGCGGTGCGGCAGGTCCGGCCCTTTCGCGGGTAAACCCGCGAAAGGGCCGGCAAATCCTGATCAGGGCTCAAACCCTTCCACAATGATCACCTCAGCCTTCGCCACCCCTTGGCGATGGCTGCa                                                           >  1:583785/1‑150 (MQ=255)                                                                                            ccGCAGGCTTCAATGAATCTGCCAGCGGTGCGGCAGGTCCGGCCCTTTCGCGGGTAAACCCGCGACAGGGCCGGCCCAGCCTGCTCAGGGCTCGGACTCTTCCACAATGATCACCTCAGCCTTCGCCACCCCTTGGCGATGGCTGCATGc                                                        <  1:449683/150‑1 (MQ=255)                                                                                                   cTTCAATGAATCTGCCAGCGGTGCGGCAGGTCCGGCCCTTTCGCGGGTAAACCCGCGAAAGGGCCGGCAAATCCTGCTCAGGGCTCAAACCCTTCCACAATGATCACCTCAGCCTTCGCCACCCCTTg                                                                       <  2:19537/128‑1 (MQ=255)                                                                                                   cTTCAATGAATCTGCCAGCGGTGCGGCAGGTCCGGCCCTTTCGCGGGTAAACCCGCGAAAGGGCCGGCAAATCCTGCTCAGGGCTCAAACCCTTCCACAATGATCACCTCAGCCTTCGCCACCCCTTg                                                                       >  1:19537/1‑128 (MQ=255)                                                                                                    ttCAATGAATCTGCCAGCGGTGCGGCAGGTCCGGCCCTTTCGCGGGTAAACCCGCGGAAGGGCCGGCAAATCCTGCTCAGGGCTCAAAcc                                                                                                            <  1:1189108/90‑1 (MQ=255)                                                                                                    ttCAATGAATCTGCCAGCGGTGCGGCAGGTCCGGCCCTTTCGCGGGTAAACCCGCGAAGGGGCCGGCAAATCCTGCTCAGGGCTCAAAcc                                                                                                            >  2:1189108/1‑90 (MQ=255)                                                                                                       aaTGAATCTGCCAGCGGTGCGGCAGGTCCGGCCCTTTCGCGGGTAAACCCGCGAAAGGGCCGGCAAATCCTGCTCAGGGCTCAAACCCTTCCACAATGATCACCTCAGCCTTCg                                                                                 >  1:73369/1‑114 (MQ=255)                                                                                                       aaTGAATCTGCCAGCGGTGCGGCAGGTCCGGCCCTTTCGCGGGTAAACCCGCGAAAGGGCCGGCAAATCCTGCTCAGGGCTCAAACCCTTCCACAATGATCACCTCAGCCTTCg                                                                                 <  2:73369/114‑1 (MQ=255)                                                                                                                            tagggTCCTTCCCTTTCGCGGGTCAACCCTGGAAGTGGCCGGCAAATCTTGCTCAGCGCTCACACCCTTCCACAATGATCACCTCAGCCTTCGCCACCCCTTGGCGATGGCTGCATGCCTCTTGGTACAGCGCGGAGCGGTAACAGGCGa                        <  2:366672/147‑1 (MQ=255)                                                                                                                                    ggCCCTTTCGCGGGTAAACCCGCGAAAGGGCCGGCAAATCCTGCTCAGGGCTCAAACCCTTCCACAATGATCACCTCAGCCTTCGCCACCCCTTGGCGATGGCTGCATGCCTCTTGGTACAGCGCGGAGCGGTAACAGGCGACGGCCTgc                >  2:1148414/1‑150 (MQ=255)                                                                                                                                       ccTTTCGCGGGTTTACCCGCGAAAGGGCCGGCAAATCCTGCTCAGGGCTCAAACCCTTCCACAATGATCACCTACGTCTTCGCCACCCCTTGGCGATGGCTGCATGCCTCTTGGTACAGCGCGGAGCGGTAACAGGCGACGGCCTGcgcg             >  1:79423/1‑150 (MQ=255)                                                                                                                                         tttCGCGGGTAAACCCGCGAAAGGGCCGGCAAATCCTGCTCAGGGCTCAAACCCTTCCACAATGATCACCTCAGCCTTCGCCACCCCTTGGCGATGGCTGCATGCCTCTTGGTACAGCGCGGAGCGGTAACAGGCGACGGCCTGCGCGTa           >  1:446165/1‑150 (MQ=255)                                                                                                                                                  tAAACCCGCGAAAGGGCCGGCAAATCCTGCTCAGGGCTCAAACCCTTCCACAATGATCACCTCAGCCTTCGCCACCCCTTGGCGATGGCTGCATGCCTCTTGGTACAGCGCGGAGCGGTAACAGGCGACGGCCTGCGCGTACGAATCGaa  <  2:1023499/150‑1 (MQ=255)                                                                                                                                                   |                                                                                                                                                     GGCGGGCAACGAACTTAAAGTGCATCTGCAGTGAGGCGATGAGCAATAGACCCGCCTGGGCGGGTCTTTTGCTTCTATTTGCGGAAACAGCCCGCAGGCTTCAATGAATCTGCCAGCGGTGCGGCAGGTCCGGCCCTTTCGCGGGTAAACCCGCGAAAGGGCCGGCAAATCCTGCTCAGGGCTCAAACCCTTCCACAATGATCACCTCAGCCTTCGCCACCCCTTGGCGATGGCTGCATGCCTCTTGGTACAGCGCGGAGCGGTAACAGGCGACGGCCTGCGCGTACGAATCGAA  >  NC_002947/4338391‑4338685

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑