| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 4,415,076 | T→C | 8.3% | intergenic (+141/+27) | PP_5644 → / ← PP_3899 | membrane protein/hicB family protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 4,415,076 | 0 | T | C | 8.3% | 135.9 / 2.7 | 48 | intergenic (+141/+27) | PP_5644/PP_3899 | membrane protein/hicB family protein |
| Reads supporting (aligned to +/- strand): ref base T (25/19); new base C (2/2); total (27/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.66e-01 | |||||||||||
ATGGCTATGAAGCACTCCTCTTGAAGCCGCCATCAAGTGATCCCACAGTCCGCCTCGGCGGGCTTCACCATGCATTCACGCTTTTTTCACGCCCTCCCCTGCACAGTGAAGGCTCATCCGCTTCCCTACGTTAGCCCGCATTGCAGTGCGGGCTTTTCTTTGGGCGGCTACGCGTGATGACCCAGGTA‑CTCACTAAGCGCTTTGCGTGTCAGATCATTCAAGGAAATACTTTGCTTGGTGGCTGCCACGCTGGCCGCTAGGTGCAGGTCGTGCCCTACCCTCACATTGAA > NC_002947/4414936‑4415225 | attgCTATCAAACACTCCGCTTGAAGCCGCCATCAAGTGATCCCACAGTCCGCCTCGGCGGGCTTCACCATGCATTCACGCTTCTTTCACGCCCCCCCCTGCACAGTGAAGGCTCAGCCGCTTCCCTCCGTTAGCCCGCACTGCAATGCg < 2:466486/147‑1 (MQ=255) tATGAAGCACTCCTCTTGAAGCCGCCATCAAGTGATCCCACAGTCCGCCTCGGCGGGCTTCACCATGCATTCACGCTTTTTTCACGCCCTCCCCTGCACAGTGAAGGCTCATCCGCTTCCCTACGTTAGCCCGCATTGCAGTGCGGGCtt > 2:509510/1‑150 (MQ=255) aTGAAGCACTCCTCTTGAAGCCGCCATCAAGTGATCCCACAGTCCGCCTCGGCGGGCTTCACCATGCATTCACGCTTTTTTCACGCCCTCCCCTGCACAGTGAAGGCTCATCCGCTTCCCTACGTTAGCCCGCATTg < 1:163578/137‑1 (MQ=255) aTGAAGCACTCCTCTTGAAGCCGCCATCAAGTGATCCCACAGTCCGCCTCGGCGGGCTTCACCATGCATTCACGCTTTTTTCACGCCCTCCCCTGCACAGTGAAGGCTCATCCGCTTCCCTACGTTAGCCCGCATTg > 2:163578/1‑137 (MQ=255) aTGAAGCACTCCTCTTGAAGCCGCCATCAAGTGATCCCACAGTCCGCCTCGGCGGGCTTCACCATGCATTCACGCTTTTTTCACGCCCTCCCCTGCACAGTGAAGGCTCATCCGCTTCCCTACGTTAGCCCGCACTGCAATGCGGGCtaa < 1:29177/150‑3 (MQ=255) aaGCACTCCTCTTGAAGCCGCCATCAAGTGATCCCACAGTCCGCCTCGGCGGGCTTCACCATGCATTCACGCTTTTTTCACGCCCTCCCCTGCACAGTGAAGGCTCATCCGCTTCCCTACGTTAGCCCGCATTGCAGTGCGGGCTTTTCt < 1:1142911/150‑1 (MQ=255) tctTGAAGCCGCCATCAAGTGATCCCACAGTCCGCCTCGGCGGGCTTCACCATGCATTCACGCTTTTTTCACGCCCTCCCCTGCACAGTGAAGGCTCATCCGCTTCCCTACGTTAGCCCGCATTGCAGTGCGGGCTTTTCttt < 1:854802/143‑1 (MQ=255) tctTGAAGCCGCCATCAAGTGATCCCACAGTCCGCCTCGGCGGGCTTCACCATGCATTCACGCTTTTTTCACGCCCTCCCCTGCACAGTGAAGGCTCATCCGCTTCCCTACGTTAGCCCGCATTGCAGTGCGGGCTTTTCttt > 2:854802/1‑143 (MQ=255) gcggccATCAAGTGATCCCACAGTCCGCCTCGGCGGGCTTCACCATGCATTCACGC‑TTTTTCACGCCCTCCCCTGCACAGTGAAGGCTCATCCGCTTCCCTACGTTAGCCCGCATTGCa > 1:1206347/4‑119 (MQ=255) gccgccATCAAGTGATCCCACAGTCCGCCTCGGCGGGCTTCACCATGCATTCACGC‑TTTTTCACGCCCTCCCCTGCACAGTGAAGGCTCATCCGCTTCCCTACGTTAGCCCGCATTGCa < 2:1206347/119‑1 (MQ=255) aGTGATCCCACAGTCCGCCTCGGCGGGCTTCACCATGCATTCACGCTTTTTTCACGCCCTCCCCTGCACAGTGAAGGCTCATCCGCTTCCCTACGTTAGCCCGCATTGCAGTGCGGGCTTTTCTTTGGGCGGCTACGCGTGATGACCCAg < 1:509510/150‑1 (MQ=255) gTGATCCCACAGTCCGCCTCGGCGGGCTTCACCATGCATTCACGCTTTTTTCACGCCCTCCCCTGCACAGTGAAGGCTCATCCGCTTCCCTACGTTAGCCCGCATTGCAGTGCGGGCTTTTCTTTGGGCGGCTACGCGTGATGACCCAgg > 2:929989/1‑150 (MQ=255) gTGATCCCACAGTCCGCCTCGGCGGGCTTCACCATGCATTCACGCTTTTTTCACGCCCTCCCCTGCACAGTGAAGGCTCATCCGCTTCCCTACGTTAGCCCGCATTGCAGTGCGGGCTTTTCTTTGGGCGGCTACGCGTGATGACCCAgg > 1:718577/1‑150 (MQ=255) gCCTCGGCGGGCTTCACCATGCATTCACGCTTTTTTCACGCCCTCCCCTGCACAGTGAAGGCTCATCCGCTTCCCTACGTTAGCCCGCATTGCAGTGCGGGCTTTTCTTTGGGCGGCTACGCGTGATGACCCAGGTA‑CTCACTAAGCGCt > 1:288552/1‑150 (MQ=255) tCGGCGGGCTTCACCATGCATTCACGCTTTTTTCACGCCCTCCCCTGCACAGTGAAGGCTCATCCGCTTCCCTACGTTAGCCCGCATTGCAGTGCGGGCTTTTCTTTGGGCGGCTACGCGTGATGACCCAGGTA‑CTCACTAAGCGCtttt < 1:929989/150‑2 (MQ=255) gcggGCTTCACCATGCATTCACGCTTTTTTCACGCCCTCCCCTGCACAGTGAAGGCTCATCCGCTTCCCTACGTTAGCCCGCATTGCAGTGCGGGCTTTTCTTTGGGCGGCTACGCGTGATGACCCAGGTA‑CTCACTAAGCGCTTTGCgt < 2:288552/150‑1 (MQ=255) gCTTCACCATGCATTCACGCTTTTTTCACGCCCTCCCCTGCACAGTGAAGGCTCATCCGCTTCCCTACGTTAGCCCGCATTGCAGTGCGGGCTTTTCTTTGGGCGGCTACGCGTGATGACCCAGGTA‑CTCACTAAGCGCTTTGCGTGTCa > 1:847423/1‑150 (MQ=255) cTTCACCATGCATTCACGCTTTTTTCACGCCCTCCCCTGCACAGTGAAGGCTCATCCGCTTCCCTACGTTAGCCCGCATTGCAGTGCGGGCTTTTCTTTGGGCGGCTACGCGTGATGACCCAGGTA‑CTCACTAAGCGCt > 1:951527/1‑139 (MQ=255) cTTCACCATGCATTCACGCTTTTTTCACGCCCTCCCCTGCACAGTGAAGGCTCATCCGCTTCCCTACGTTAGCCCGCATTGCAGTGCGGGCTTTTCTTTGGGCGGCTACGCGTGATGACCCAGGTA‑CTCACTAAGCGCt < 2:951527/139‑1 (MQ=255) ttCACCATGCATTCACGCTTTTTTCACGCCCTCCCCTGCACAGTGAAGGCTCATCCGCTTCCCTACGTTAGCCCGCATTGCAGTGCGGGCTTTTCTTTGGGCGGCTACGCGTGATGACCCAGGTA‑CTCACTAAGCGCTTTGCGTGTCAGa > 2:104403/1‑150 (MQ=255) cATTCACGCTTTTTTCACGCCCTCCCCTGCACAGTGAAGGCTCATCCGCTTCCCTACGTTAGCCCGCATTGCAGTGCGGGCTTTTCTTTGGGCGGCTACGCGTGATGACCCAGGTA‑CTCACTAAGCGCTTTGCGTGTCAGATCATTCAAg < 2:1095935/150‑1 (MQ=255) aTTCACGCTTTTTTCACGCCCTCCCCTGCACAGTGAAGGCTCATCCGCTTCCCTACGTTAGCCCGCATTGCAGTGCGGGCTTTTCTTTGGGCGGCTACGCGTGATGACCCAg > 2:618873/1‑112 (MQ=255) aTTCACGCTTTTTTCACGCCCTCCCCTGCACAGTGAAGGCTCATCCGCTTCCCTACGTTAGCCCGCATTGCAGTGCGGGCTTTTCTTTGGGCGGCTACGCGTGATGACCCAg < 1:618873/112‑1 (MQ=255) aTTCACGCTTTTTTCACGCCCTCCCCTGCACAGTGAAGGCTCATCCGCTTCCCTACGTTAGCCCGCATTGCAGTGCGGGCTTTTCTTTGGGCGGCTACGCGTGATGACCCAGGTA‑CTCACTAAGCGCTTTGCGTGTCAGATCATTCAAgg > 1:809728/1‑150 (MQ=255) ttCACGCTTTTTTCACGCCCTCCCCTGCACAGTGAAGGCTCATCCGCTTCCCTACGTTAGCCCGCATTGCAGTGCGGGCTTTTCTTTGGGCGGCTACGCGTGATGACCCAGGTA‑CTCACTAAGCGCTTTGCGTGTCAGATCATTCAAGGa > 1:650841/1‑150 (MQ=255) aCGCTTTTTTCACGCCCTCCCCTGCACAGTGAAGGCTCATCCGCTTCCCTACGTTAGCCCGCATTGCAGTGCGGGCTTTTCTTTGGGCGGCTACGCGTGATGACCCAGGTA‑CTCACTAAGCGCTTTGCGTGTCAGATCATTCAAGGAAAt > 2:1052088/1‑150 (MQ=255) ttttCACGCCCTCCCCTGCACAGTGAAGGCTCATCCGCTTCCCTACGTTAGCCCGCATTGCAGTGCGGGCTTTTCTTTGGGCGGCTACGCGTGATGACCCAGGTA‑CTCACTAAGCGCTTTGCGTGTCAGATCATTCAAGGAAATACTTTg < 2:478819/150‑1 (MQ=255) tCACGCCCTCCCCTGCACAGTGAAGCCTCATCCGCTTCCCTACGTTAGCCCGCATTGCAGTGCGGGCTTTTCTTTGGGCGGCTACGCGTGATGACCCAGGTA‑CTCACTAAGCGCTTTGCGTGTCAGATCATTCAAGGAAATACTTTGCtt < 2:1057216/150‑1 (MQ=255) ccTCCCCTGCACAGTGAAGGCTCATCCGCTTCCCTACGTTAGCCCGCATTGCAGTGCGGGCTTTTCTTTGGGCGGCTACGCGt > 1:128110/1‑83 (MQ=255) ccTCCCCTGCACAGTGAAGGCTCATCCGCTTCCCTACGTTAGCCCGCATTGCAGTGCGGGCTTTTCTTTGGGCGGCTACGCGt < 2:128110/83‑1 (MQ=255) cTCCCCTGCACAGTGAAGGCTCATCCGCTTCCCTACGTTAGCCCGCATTGCAGTGCGGGCTTTTCTTTGGGCGGCTACGCGTGATGACCCAGGTA‑CTCACTAAGCGCTTTGCGTGTCAGATCATTCAAGGAAATACTTTGCTTGGTGGCt < 2:718577/150‑1 (MQ=255) aGTGAAGGCACATCCTCTTCCCGACGTTAGCCCGCATTGCATTGCGGGCTTTTCTTTGGGCGGATACGAGTGATGCAAAAGGTATCCGCCTATACCCGCTG‑GGCTGACTGCAGGCAAGGAAATACTTGGCTTGGTGGCTGCCACGCTGGc > 2:1168471/1‑150 (MQ=255) tCATCCGCTTCCCTACGTTAGCCCGCATTGCAGTGCGGGCTTTTCTTTGGGCGGCTACGCGTGATGACCCAGGTA‑CTCACTAAGCGCTTTGCGTGTCAGATCATTCAAGGAAATACTTTGCTTGGTGGCTGCCACGCTGGCCGCTAGGTg > 2:1099298/1‑150 (MQ=255) cGCTTCCCTACGTTAGCCCGCATTGCAGTGCGGGCTTTTCTTTGGGCGGCTACGCGTGATGACCCAGGTA‑CTCACTAAGCGCTTTGCGTGTCAGATCATTCAAGGAAATACTTTGCTTGGTGGCTGCCACGCTGGCCGCTAGGTGCAg < 2:847437/148‑1 (MQ=255) cGCTTCCCTACGTTAGCCCGCATTGCAGTGCGGGCTTTTCTTTGGGCGGCTACGCGTGATGACCCAGGTA‑CTCACTAAGCGCTTTGCGTGTCAGATCATTCAAGGAAATACTTTGCTTGGTGGCTGCCACGCTGGCCGCTAGGTGCAg > 1:847437/1‑148 (MQ=255) gCTTCCCTACGTTAGCCCGCATTGCAGTGCGGGCTTTTCTTTGGGCGGCTACGCGTGATGACCCAGGTA‑CTCACTAAGCGCTTTGCGTGTCAGATCATTCAAGGAAATACTTTGCTTGGTGGCTGCCACGCTGGCCGCTAg < 1:653285/141‑1 (MQ=255) gCTTCCCTACGTTAGCCCGCATTGCAGTGCGGGCTTTTCTTTGGGCGGCTACGCGTGATGACCCAGGTA‑CTCACTAAGCGCTTTGCGTGTCAGATCATTCAAGGAAATACTTTGCTTGGTGGCTGCCACGCTGGCCGCTAg > 2:653285/1‑141 (MQ=255) tCCCTACGTTAGCCCGCATTGCAGTGCGGGCTTTTCTTTGGGCGGCTACGCGTGATGACCCAGGTA‑CTCACTAAGCGCTTTGCGTGTCAGATCATTCAAGGAAATACTTTGCTTGGTGGCTGCCACGCTGGCCGCTAGGTGCAGGTCGTg < 1:1099298/150‑1 (MQ=255) tACGTTAGCCCGCATTGCAGTGCGGGCTTTTCTTTGGGCGGCTACGCGTGATGACCCAGGTA‑CTCACTAAGCGCTTTGCGTGTCAGATCATTCAAGGAAATACTTTGCTTGGTGGCTGCCACGCTGGCCGCTAGGTGCAGGTCGTGCCCt > 2:851754/1‑150 (MQ=255) aaGCCCGCACTGCAATGCGGGCTTTTCTTTGGGCGGCTACGCGTGATGACCCAGGTA‑CTCACTAAGCGCTTTGCGTGTCAGATCATTCAAGGAAATACTTTGCTTGGTGGCTGCCACGCTGGCCGCTAGGTGCAGGTCGTGCCCTACCCt > 1:1097649/2‑150 (MQ=255) gCCCGCACTGCAATGCGGCCTTTTCTTTGGGCGGCTACGCGTGATGACCCAGGTA‑CTCACTAAGCGCTTTGCGTGTCAGATCATTCAAGGAAATACTTTGCTTGGTGGCTGCCACGCTGGCCGCTAGGTGCAGGTCGTGCCCTACCCTca > 1:699834/1‑150 (MQ=255) ccGCATTGCAGTGCGGGCTTTTCTTTGGGCGGCTACGCGTGATGACCCAGGTA‑CTCACTAAGCGCTTTGCGTGTCAGATCATTCAAGGAAATACtt > 1:918643/1‑96 (MQ=255) ccGCATTGCAGTGCGGGCTTTTCTTTGGGCGGCTACGCGTGATGACCCAGGTA‑CTCACTAAGCGCTTTGCGTGTCAGATCATTCAAGGAAATACtt < 2:918643/96‑1 (MQ=255) cGCATTGCAGTGCGGGCTTTTCTTTGGGCGGCTACGCGTGATGACCCAGGTA‑CTCACTAAGCGCTTTGCGTGTCAGATCATTCAAGGAAATACTTTGCTTGGTGGCTGCCACGCTGGCCGCTAGGTGCAGGTCGTg > 2:655956/1‑136 (MQ=255) cGCATTGCAGTGCGGGCTTTTCTTTGGGCGGCTACGCGTGATGACCCAGGTA‑CTCACTAAGCGCTTTGCGTGTCAGATCATTCAAGGAAATACTTTGCTTGGTGGCTGCCACGCTGGCCGCTAGGTGCAGGTCGTg < 1:655956/136‑1 (MQ=255) cGCATTGCAGTGCGGGCTTTTCTTTGGGCGGCTACGCGTGATGACCCAGGTA‑CTCACTAAGCGCTTTGCGTGTCAGATCATTCAAGGAAATACTTTGCTTGGTGGCTGCCACGCTGGCCGCTAGGTGCAGGTCGTGCCCTACCCTCACAt > 2:354669/1‑150 (MQ=255) cGCATTGCAGTGCGGGCTTTTCTTTGGGCGGCTACGCGTGATGACCCAGGTA‑CTCACTAAGCGCTTTGCGTGTCAGATCATTCAAGGAAATACTTTGCTTGGTGACTGCCACGCTGGCCGCTAGGTGCAGGTCGTGCCCTACCCTCACAt > 1:23763/1‑150 (MQ=255) cATTGCAGTGCGGGCTTTTCTTTGGGCGGCTACGCGTGATGACCCAGGTA‑CTCACTAAGCGCTTTGCGTGTCAGATCATTCAAGGAAATACTTTGCTTGGTGGCTGCCACGCTGGCCGCTAGGTGCATGTCGTGCCCTACCCTCTCAtgt > 2:974891/1‑148 (MQ=255) ttGCAGTGCGGGCTTTTCTTTGGGCGGCTACGCGTGATGACCCAGGTA‑CTCACTAAGCGCTTTGCGTGTCAGATCATTCAAGGAAATACTTTGCTTGGTGGCTGCCACGCTGGCCGCTAGGTGCAGGTCGTGCCCTACCCTCACATTGaa < 2:847423/150‑1 (MQ=255) | ATGGCTATGAAGCACTCCTCTTGAAGCCGCCATCAAGTGATCCCACAGTCCGCCTCGGCGGGCTTCACCATGCATTCACGCTTTTTTCACGCCCTCCCCTGCACAGTGAAGGCTCATCCGCTTCCCTACGTTAGCCCGCATTGCAGTGCGGGCTTTTCTTTGGGCGGCTACGCGTGATGACCCAGGTA‑CTCACTAAGCGCTTTGCGTGTCAGATCATTCAAGGAAATACTTTGCTTGGTGGCTGCCACGCTGGCCGCTAGGTGCAGGTCGTGCCCTACCCTCACATTGAA > NC_002947/4414936‑4415225 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |