Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 289,943 C→A 8.1% intergenic (+26/‑140) oprE → / → PP_0235 outer‑membrane porin E/peroxidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947289,9430CA 8.1% 153.3 / 2.5 49intergenic (+26/‑140)oprE/PP_0235outer‑membrane porin E/peroxidase
Reads supporting (aligned to +/- strand):  ref base C (22/23);  new base A (2/2);  total (24/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.13e-01

GGAAGGCACTCTCAAAGGCCTGGGCTTCAGCCTGCGTCAAGGTGTCTACCGTGGTAGCGGCGAAAGCGCCCTGGACAAAGACCAGACTCGCTTCATCGTGAACTACACTTACAACTTCCTGTAAGCCGTAGCTGAAAAAGAAGCCTCGCCTGATGCGAGGCTTTTTTGTGCCTGCAGATTTATATGTATTTTGGTTATAAATAAATTGTTATTTATTCTTTTTGTTTTTAATCGATTGCAGGCACATTGAACCCATACGGTACAGAACCCAGCCCAGGAGCCGCAGCCCATG  >  NC_002947/289794‑290085
                                                                                                                                                     |                                                                                                                                              
ggAAGGCACTCTCAAAGGCCTGGGCTTCAGCCTGCGTCAAGGTGTCTACCGTGGTAGCGGCGAAAGCGCCCTGGACAAAGACCAGACTCGCTTCATCGTGAACTACACTTACAACTTCCTGTAAGCCGTAGCTGAAAAAGAAGGCTCGcc                                                                                                                                                <  2:926991/150‑1 (MQ=255)
        ctctCAAAGGCCTGGGCTTCAGCCTGCGTCAAGGTGTCTACCGTGGTAGCGGCGAAAGCGCCCTGGACAAAGACCAGACTCGCTTCATCGTGAACTACACTTACAACTTCCTGTAAGCCGTAGCTGAAAAAGAAGCCTCGCCTGATGCGa                                                                                                                                        >  2:581289/1‑150 (MQ=255)
         tctcAAAGGCCTGGGCTTCAGCCTGCGTCAAGGTGTCTACCGTGGTAGCGGCGAAAGCGCCCTGGACAAAGACCAGACTCGCTTCATCGTGAACTACACTTACAACTTCCTGTAAGCCGTAGCTGAAAAAGAAGCCTCGCCTGATGCGAg                                                                                                                                       >  1:320522/1‑150 (MQ=255)
               aGGCCTGGGCTTCAGCCTGCGTCAAGGTGTCTACCGTGGTAGCGGCGAAAGCGCCCTGGACAAAGACCAGACTCGCTTCATCGTGAACTACACTTACAACTTCCTGTAAGCTGTAGCTGAAAAAGAAGCCTCGCCTGATGCGAGGCtttt                                                                                                                                 <  2:834730/150‑1 (MQ=255)
                ggCCTGGGCTTCAGCCTGCGTCAAGGTGTCTACCGTGGTAGCGGCGAAAGCGCCCTGGACAAAGACCAGACTCGCTTCATCGTGAACTACACTTACATCTTCCTGTAAGCCGTAGCTGAAAAAGAAGCCTCGCCTGATGCGAGGCttttt                                                                                                                                <  1:548364/150‑1 (MQ=255)
                 gCCTGGGCTTCAGCCTGCGTCAAGGTGTCTACCGTGGTAGCGGCGAAAGCGCCCTGGACAAAGACCAGACTCGCTTCATCGTGAACTACACTTACAACTTCCTGTAAGCCGTAGCTGAAAAAGAAGCCTCGCATCAGGCGAGGCttcttt                                                                                                                               <  2:243086/150‑1 (MQ=255)
                   cTGGGCTTCAGCCTGCGTCAAGGTGTCTACCGTGGTAGCGGCGAAAGCGCCCTGGACAAAGACCAGACGGTTGTCATCGTGAACTACACTTACAACTTCCTGTAAGCCGTAGCTGAAAAAGAAGCCTCGCCTGATGCGAGGCTTTTTtgt                                                                                                                             >  1:687422/1‑150 (MQ=255)
                      ggCTTCAGCCTGCGTCAAGGTGTCTACCGTGGTAGCGGCGAAAGCGCCCTGGACAAAGACCAGACTCGCTTCATCGTGAACTACACTTACAACTTCCTGTAAGCCGTAGCTGAAAAAGAAGCCTCGCCTGATGCGAGGCTTTTTTGTGcc                                                                                                                          >  2:1037958/1‑150 (MQ=255)
                                  cGTCAAGGTGTCTACCGTGGTAGCGGCGAAAGCGCCCTGGACAAAGACCAGACTCGCTTCATCGTGAACTACACTTACAACTTCCTGTAAGCCGTAGCTGAAAAAGAAGCCTCGCCTGATGCGAGGCTTTTTTGTGCCTGCAGATTtata                                                                                                              <  1:1194537/150‑1 (MQ=255)
                                    tCAAGGTGTCTACCGTGGTAGCGGCGAAAGCGCCCTGGACAAAGACCAGACTCGCTTCATCGTGAACTACACTTACAACTTCCTGTAAGCCGTAGCTGAAAAAGAAGCCTCGCCTGATGCGAGGCtttttt                                                                                                                               >  1:311180/1‑131 (MQ=255)
                                    tCAAGGTGTCTACCGTGGTAGCGGCGAAAGCGCCCTGGACAAAGACCAGACTCGCTTCATCGTGAACTACACTTACAACTTCCTGTAAGCCGTAGCTGAAAAAGAAGCCTCGCCTGATGCGAGGCtttttt                                                                                                                               <  2:311180/131‑1 (MQ=255)
                                           gtCTACCGTGGTAGCGGCGAAAGCGCCCTGGACAAAGACCAGACTCGCTTCATCGTGAACTACACTTACAACTTCCTGTAAGCCGTAGCTGAAAAAGAAGCCTCGCCTGATGCGAGGCTTTTTTGTGCCTGCAGATTTATATGTATTTTg                                                                                                     <  2:846287/150‑1 (MQ=255)
                                             cTACCGTGGTAGCGGCGAAAGCGCCCTGGACAAAGACCAGACTCGCTTCATCGTGAACTACACTTACAACTTCCTGTAAGCCGTAGCTGAAAAAGAAGCCTCGCCTGATGCGAGGCTTTTTTGTGCCTGCAGATTTATATGTATTTTGGt                                                                                                   <  1:581289/150‑1 (MQ=255)
                                                ccGTGGTAGCGGCGAAAGCGCCCTGGACAAAGACCAGACTCGCTTCATCGTGAACTACACTTACAACTTCCTGTAAGCCGTAGCTGAAAAAGAAGCCTCGCCTGATGCGAGGCTTTTTTGTGCCTGCAGATTTATATGTATTTTg                                                                                                     <  1:845104/145‑1 (MQ=255)
                                                ccGTGGTAGCGGCGAAAGCGCCCTGGACAAAGACCAGACTCGCTTCATCGTGAACTACACTTACAACTTCCTGTAAGCCGTAGCTGAAAAAGAAGCCTCGCCTGATGCGAGGCTTTTTTGTGCCTGCAGATTTATATGTATTTTg                                                                                                     >  2:845104/1‑145 (MQ=255)
                                                  gTGGTAGCGGCGAAAGCGCCCTGGACAAAGACCAGACTCGCTTCATCGTGAACTACACTTACAACTTCCTGTAAGCCGTAGCTGAAAAAGAAGCCTCGCCTGATGCGAGGCTTTTTTGTGCCTGCAGATTTATATGTATTTTGGTTataa                                                                                              >  2:916359/1‑150 (MQ=255)
                                                             gAAAGCGCCCTGGACAAAGACCAGACTCGCTTCATCGTGAACTACACTTACAACTTCCTGTAAGCCGTAGCTGAAAAAGAAGCCTCGCCTGATGCGAGGCtttt                                                                                                                                 <  2:435899/104‑1 (MQ=255)
                                                             gAAAGCGCCCTGGACAAAGACCAGACTCGCTTCATCGTGAACTACACTTACAACTTCCTGTAAGCCGTAGCTGAAAAAGAAGCCTCGCCTGATGCGAGGCtttt                                                                                                                                 >  1:435899/1‑104 (MQ=255)
                                                                    cccTGGACAAAGACCAGACTCGCTTCATCGTGAACTACACTTACAACTTCCTGTAAGCCGTAGCTGAAAAAGAAGCCTCGCCTGATGCGAGGCTTTTTTGTGCCTGCAGATTTATATGTATTTTGGTTATAAATAAATTGTTATTTATTc                                                                            <  2:1169303/150‑1 (MQ=255)
                                                                         gACAAAGACCAGACTCGCTTCATCGTGAACTACACTTACAACTTCCTGTAAGCCGTAGCTGAAAAAGAAGCCTCGCCTGATGCGAGGCTTTTTTGTGCCTGCAGATTTATATGTATTTTGGTTATAAATAAATTGTTATTTATTCttttt                                                                       <  2:320522/150‑1 (MQ=255)
                                                                           cAAAGACCAGACTCGCTTCATCGTGAACTACACTTACAACTTCCTGTAAGCCGTAGCTGAAAAAGAAGCCTCGCCTGATGCGAGGCTTTTTTGTGCCTGCAGATTTATATGTATTTTGGTTATAAATAAATTGTTATTTATTCTTTTTGt                                                                     >  2:492079/1‑150 (MQ=255)
                                                                                            tCATCGTGAACTACACTTACAACTTCCTGTAAGCCGTAGCTGAAAAAGAAGCCTCGCCTGATGCGAGGCTTTTTTGTGCCTGCAGATTTATATGTATTTTGGTTATAAATAAATTGTTATTTATTCTTTTTGTTTTTAATCGATTGCAgg                                                    <  2:687422/150‑1 (MQ=255)
                                                                                               tCGTGAACTACACTTACAACTTCCTGTAAGCCGTAGCTGAAAAAGAAGCCTCGCCTGATGCGAGGCTTTTTTGTGCCTGCAGATTTATATGTATTTTGGTTATAAATAAATTGTTATTTATTCTTTTTGTTTTTATTCGATTTCAggttc                                                 >  2:359100/1‑147 (MQ=255)
                                                                                                 gTGAACTACACTTACAACTTCCTGTAAGCCGTAGCTGAAAAAGAAGCCTCGCCTGATGCGAGGCTTTTTTGTGCCTGCAGATTTATATGTATTTTGGTTATAAATAAATTGTTATTTATTCTTTTTGTTTTTAATCGATTGCAGGCACAt                                               <  2:708759/150‑1 (MQ=255)
                                                                                                          acTTACAACTTCCTGTAAGCCGTAGCTGAAAAAGAAGCCTCGCCTGATGCGAGGCTTTTTTGTGCCTGCAGATTTATATGTATTTTGGTTATAAATAAATTGTTATTTATTCttt                                                                         <  1:253198/115‑1 (MQ=255)
                                                                                                          acTTACAACTTCCTGTAAGCCGTAGCTGAAAAAGAAGCCTCGCCTGATGCGAGGCTTTTTTGTGCCTGCAGATTTATATGTATTTTGGTTATAAATAAATTGTTATTTATTCttt                                                                         >  2:253198/1‑115 (MQ=255)
                                                                                                             tACAACTTCCTGTAAGCCGTAGCTGAAAAAGAAGCCTCGCCTGATGCGAGGCTTTTTTGTGCCTGCAGATTTATATGTATTTTGGTTATAAATAAATTGTTATTTATTCTTTTTGTTTTTAATCGATTGCAGGCACATTGAACCCATACg                                   <  1:844901/150‑1 (MQ=255)
                                                                                                                aaCTTCCTGTAAGCCGTAGCTGAAAAAGAAGCCTCGCCTGATGCGAGGCTTTTTTGTGCCTGCAGATTTATATGTATTTTGGTTATAAATAAATTGTTATTTATTCTTTTTGTTTTTAATCGATTGCAGGCACATTGAACCCATACGGTa                                >  2:735789/1‑150 (MQ=255)
                                                                                                                 aCTTCCTGTAAGCCGTAGCTGAAAAAGAAGCCTCGCCTGATGCGAGGCTTTTTTGTGCCTGCAGATTTATATGTATTTTGGTTATAAATAAATTGTTATTTATTCTTTTTGTTTTTAATCGATTGCAGGCACATTGAACCCATACGGTAc                               <  1:916359/150‑1 (MQ=255)
                                                                                                                                    tGAAAAAGAAGCCTCGCCTGATGCGAGGCTTTTTTGTGCCTGCAGATTTATATGTATTTTGGTTATAAATAAATTGTTATTTATTCTTTTTGTTTTTAATCGATTGCAGGCACATTGAACCCATACGGTACAGAACCCAGCCCAGGAGcc            >  2:492330/1‑150 (MQ=255)
                                                                                                                                      aaaaaGAAGCCTCGCCTGATGCGAGGCTTTTTTGTGCCTGCAGATTTATATGTATTTTGGTTATAAATAAATTGTTATTTATTCTTTTTGTTTTTAATCGATTGCAGGCACATTGAACCCATACg                                   <  1:1144063/125‑1 (MQ=255)
                                                                                                                                      aaaaaGAAGCCTCGCCTGATGCGAGGCTTTTTTGTGCCTGCAGATTTATATGTATTTTGGTTATAAATAAATTGTTATTTATTCTTTTTGTTTTTAATCGATTGCAGGCACATTGAACCCATACg                                   >  2:1144063/1‑125 (MQ=255)
                                                                                                                                        aaaGAAGCCTCGCCTGATGCGAGGCTTTTTTGTGCCTGCAGATTTATATGTATTTTGGTTATAAATAAATTGTTATTTATTCTTTTTGTTTTTAATCGATTGCAGGCACATTGAACCCATACGGTACAGAACCCAGCCCAGGAGCCGCAg        <  1:1037958/150‑1 (MQ=255)
                                                                                                                                          agaagCCTCGCCTGATGCGAGGCTTTTTTGTGCCTGCAGATTTATATGTATTTTGGTTATAAATAAATTGTTATTTATTCTTTTTGTTTTTAATCGATTGCa                                                      >  1:228036/1‑102 (MQ=255)
                                                                                                                                          agaagCCTCGCCTGATGCGAGGCTTTTTTGTGCCTGCAGATTTATATGTATTTTGGTTATAAATAAATTGTTATTTATTCTTTTTGTTTTTAATCGATTGCa                                                      <  2:228036/102‑1 (MQ=255)
                                                                                                                                          agaagCCTCGCCTGATGCGAGGCACTTTTGCGCCTGCAGATTTATATGGAGTTAGGTGATAAATAAGGTAAGAAGTATTCTTTTTTTTTTGAATTGTTTTCAGGCACATTGAACCCATACGGTACAGAACCCAGCCCAGGAGCCGCAGcc      >  2:740471/1‑150 (MQ=255)
                                                                                                                                            aagCCTCGCATCAGGCGAGGCTTTTTTGTGCCTGCAGATTTATATGTATTTTGGTTATAAATAAATTGTTATTTATTCTTTTTGTTTTTAATCGATTGCAGGCACATTGAACCCATACGGTACAGAACCCAGCCCAGGAGCCGCAGCCCa    >  1:637528/1‑150 (MQ=255)
                                                                                                                                             agCCTCGCATCAGGCGAGGCTTTTTTGTGCCTGCAGATTTATATGTATTTTGGTTATAAATAAATTGTTATTTATTCTTTTTGTTTTTAATCGATTGCAGGCACATTGAACCCATACGGTACAGAACCCAGCCCAGGa               <  2:1140018/138‑1 (MQ=255)
                                                                                                                                             agCCTCGCATCAGGCGAGGCTTTTTTGTGCCTGCAGATTTATATGTATTTTGGTTATAAATAAATTGTTATTTATTCTTTTTGTTTTTAATCGATTGCAGGCACATTGAACCCATACGGTACAGAACCCAGCCCAGGa               >  1:1140018/1‑138 (MQ=255)
                                                                                                                                              gCCTCGCCTGATGCGAGGCTTTTTTGTGCCTGCAGATTTATATGTATTTTGGTTATAAATAAATTGTTATTTATTCTTTTTTTTTTTAATCGATTGCAGGCACATTGAACCCATACGGTACAGAACCCAGCCCAGGAGCCGCAGCCCATg  >  2:454315/1‑150 (MQ=255)
                                                                                                                                               ccTCGCCTGATGCGAGGCTTTTTTGTGCCTGCAGATTTATATGTATTTTGGTTATAAATAAATTGTTATTTATTCTTTTTGTTTTTAATCGATTGCAgg                                                    >  2:797712/1‑99 (MQ=255)
                                                                                                                                               ccTCGCCTGATGCGAGGCTTTTTTGTGCCTGCAGATTTATATGTATTTTGGTTATAAATAAATTGTTATTTATTCTTTTTGTTTTTAATCGATTGCAgg                                                    <  1:797712/99‑1 (MQ=255)
                                                                                                                                                cTCGCCTGATGCGAGGCTTTTTTGTGCCTGCAGATTTATATGTATTTTGGTTATAAATAAATTGTTATTTATTCTTTTTGTTTTTAATCGATTGCAGGCACATTGaa                                           >  2:1074761/1‑107 (MQ=255)
                                                                                                                                                cTCGCCTGATGCGAGGCTTTTTTGTGCCTGCAGATTTATATGTATTTTGGTTATAAATAAATTGTTATTTATTCTTTTTGTTTTTAATCGATTGCAGGCACATTGaa                                           <  1:1074761/107‑1 (MQ=255)
                                                                                                                                                 tCGCCTGATGCGAGGCTTTTTTGTGCCTGCAGATTTATATGTATTTTGGTTATAAATAAATTGTTATTTATTCtt                                                                          <  2:468988/75‑1 (MQ=255)
                                                                                                                                                 tCGCCTGATGCGAGGCTTTTTTGTGCCTGCAGATTTATATGTATTTTGGTTATAAATAAATTGTTATTTATTCtt                                                                          >  1:468988/1‑75 (MQ=255)
                                                                                                                                                 tCGCCTGATGCGAGGCTTTTTTGTGCCTGCAGATTTATATGTATTTTGGTTATAAATAAATTGTTATTTATTCTTTTTGTTTTTAATCGATTGCAGGCACATTGAACCCATACGGTACAGAACCCAGCCCAGGAGCCGCAg        <  1:329185/141‑1 (MQ=255)
                                                                                                                                                 tCGCCTGATGCGAGGCTTTTTTGTGCCTGCAGATTTATATGTATTTTGGTTATAAATAAATTGTTATTTATTCTTTTTGTTTTTAATCGATTGCAGGCACATTGAACCCATACGGTACAGAACCCAGCCCAGGAGCCGCAg        >  2:329185/1‑141 (MQ=255)
                                                                                                                                                   gCCTGATGCGAGGCTTTTTTGTGCCTGCAGATTTATATGTATTTTGGTTATAAATAAATTGTTATTTATTCTTTTTGtttt                                                                  >  1:915808/1‑81 (MQ=255)
                                                                                                                                                   gCCTGATGCGAGGCTTTTTTGTGCCTGCAGATTTATATGTATTTTGGTTATAAATAAATTGTTATTTATTCTTTTTGtttt                                                                  <  2:915808/81‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                              
GGAAGGCACTCTCAAAGGCCTGGGCTTCAGCCTGCGTCAAGGTGTCTACCGTGGTAGCGGCGAAAGCGCCCTGGACAAAGACCAGACTCGCTTCATCGTGAACTACACTTACAACTTCCTGTAAGCCGTAGCTGAAAAAGAAGCCTCGCCTGATGCGAGGCTTTTTTGTGCCTGCAGATTTATATGTATTTTGGTTATAAATAAATTGTTATTTATTCTTTTTGTTTTTAATCGATTGCAGGCACATTGAACCCATACGGTACAGAACCCAGCCCAGGAGCCGCAGCCCATG  >  NC_002947/289794‑290085

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: