Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,442,673 C→G 9.1% intergenic (‑99/+39) nicP‑II ← / ← nicT porin‑like protein/metabolite transport protein NicT

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,442,6730CG 9.1% 134.2 / 5.1 44intergenic (‑99/+39)nicP‑II/nicTporin‑like protein/metabolite transport protein NicT
Reads supporting (aligned to +/- strand):  ref base C (16/24);  new base G (2/2);  total (18/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.60e-01

GCGTGCAGACGAAGAAAAACGCGGCGCAGTGAAACCTTGCATAGGGCTACTCCTGGATTTGGGCAACAGCAACCCGGCGCGGCGGCACTGCATGGCCACCGGCGTGCGGGTTGCATCGAGAGAGGTCGGCAGGCGGGCCCCGGCCCGCCTGCCCTGCCGTTGCGTGAAGCGCTGGGCCGCTAGCGGTTGACCAGCTTGGCCGGCAAGGCGATGACCAGGAAGCAGCTGAGGATCAGGCAGCCGGCAAACACCCACAGCGCAGCGTGCGAGGTGCCGGTGAGGTCGGTGA  >  NC_002947/4442533‑4442821
                                                                                                                                            |                                                                                                                                                    
gCGTGCAGACGAAGAAAAACGCGGCGCAGTGAAACCTTGCATAGGGCTACTCCTGGATTTGGGCAACAGCAACCCGGCGCGGCGGCACTGCATGGCCACTTGCGGGCGGGTTGCATCGAGAGAGGTCGGCAGGCGGGCCCCGGCCCGcct                                                                                                                                             <  1:220705/150‑1 (MQ=255)
gCGTGCAGACGAAGAAAAACGCGGCGCAGTGAAACCTTGCATAGGGCTACTCCTGGATTTGGGCAACAGCAACCCGGCGCGGCGGCACTGCATGGCCACCGGCGTGCGGGTTGCATCGAGAGAGGTCGGCAGGCGGGCCCCGGCCCGcct                                                                                                                                             >  1:286673/1‑150 (MQ=255)
 cGTGCAGACGAAGAAAAACGCGGCGCAGTGAAACCTTGCATAGGGCTACTCCTGGATTTGGGCAACAGCAACCCGGCGCGGCGGCACTGCATGGCCACCGGCGTGCGGGTTGCATCGAGAGAGGTCGGCAGGCGGGCCGGGGCCCGcctg                                                                                                                                            <  2:386313/150‑1 (MQ=255)
          gaagaaAAACGCGGCGCAGTGAAACCTTGCATAGGGCTACTCCTGGATTTGGGCAACAGCAACCCGGCGCGGCGGCACTGCATGGCCACCGGCGTGCGGGTTGCATCGAGAGAGGTCGGCAGGCGGGCCCCGGCCCGCCTGCCCTGCCGt                                                                                                                                   >  2:1194437/1‑150 (MQ=255)
           aagaaAAACGCGGCGGAGTGAACCCTTGCATAGGGCTATTCCATGATTTGGGCAACAGCAACCCGGCGCGGCGGCACTGCATGGCCACCGGCGTGCGGGTTGCATCGAGAGAGGTCGGCAGGCGGGCCCCGGCCCGCCTGCCCTGCCGtt                                                                                                                                  <  1:615939/150‑1 (MQ=255)
            agaaAAACGCGGCGCAGTGAAACCTTGCATAGGGCTACTCCTGGATTTGGGCAACAGCAACCCGGCGCGGCGGCACTGCATGGCCACCGGCGTGCGGGTTGCATCGAGAGAGGTCGGCAGGCGGGCCGGGGCCCGcc                                                                                                                                              <  1:791203/137‑1 (MQ=255)
            agaaAAACGCGGCGCAGTGAAACCTTGCATAGGGCTACTCCTGGATTTGGGCAACAGCAACCCGGCGCGGCGGCACTGCATGGCCACCGGCGTGCGGGTTGCATCGAGAGAGGTCGGCAGGCGGGCCGGGGCCCGcc                                                                                                                                              >  2:791203/1‑137 (MQ=255)
             gaaAAACGCGGCGCAGTGAAACCTTGCATAGGGCTACTCCTGGATTTGGGCAACAGCAACCCGGCGCGGCGGCACTGCATGGCCACCGGCGTGCGGGTTGCATCGAGAGAGGTCGGCAGGCGGGCCCCGGCCCGCCTGCCCTGCCGTTGc                                                                                                                                <  2:1109260/150‑1 (MQ=255)
                  aCGCGGCGCAGTGAAACCTTGCATAGGGCTACTCCTGGATTTGGGCAACAGCAACCCGGCGCGGCGGCACTGCATGGCCACCGGCGTGCGGGTTGCATCGAGAGAGGTCGGCAGGCGGGCCCCGGCCCGCCTGCCCTGCCGTTGCGTGaa                                                                                                                           <  1:231913/150‑1 (MQ=255)
                       gcgcAGTGAAACCTTGCATAGGGCTACTCCTGGATTTGGGCAACAGCAACCCGGCGCGGCGGCACTGCATGGCCACCGGCGTGCGGGTTGCATCGAGAGAGGTCGGCAGGCGGGCCCCGGCCCGCCTGCCCTGCCGTTGCGTGAAGCGCt                                                                                                                      >  2:1100046/1‑150 (MQ=255)
                        cgcAGTGAAACCTTGCATAGGGCTACTCCTGGATTTGGGCAACAGCAACCCGGCGCGGCGGCACTGCATGGCCACCGGCGTGCGGGTTGCATCGAGAGAGGTCGGCAGGCGGGCCCCGGCCCGCCTGCCCTGCCGTTGCGTGAAGCGCTg                                                                                                                     <  1:1194437/150‑1 (MQ=255)
                         gcAGTGAAACCTTGCATAGGGCTACTCCTGGATTTGGGCAACAGCAACCCGGCGCGGCGGCACTGCATGGCCACCGGCGTGCGGGTTGCATCGAGAGAGGTCGGCAGGCGGGCCCCGGCCCGCCTGCCCTGCCGTTGCGTGAAGCGCTgg                                                                                                                    >  2:488277/1‑150 (MQ=255)
                         gcAGTGAAACCTTGCATAGGGCTACTCCTGGATTTGGGCAACAGCAACCCGGCGCGGCGGCACTGCATGGCCACCGGCGTGCGGGTTGCATCGAGAGAGGTCGGCAGGCGGGCCCCGGCCCGCCTGCCCTGCCGTTGCGTGAAGCGCTgg                                                                                                                    <  1:567716/150‑1 (MQ=255)
                              gAAACCTTGCATAGGGCGACTCCTGGATTTGGGCAACAGCAACCCGGCGCGGCGGCACTGCATGGCCACCGGCGTGCGGGTTGCATCGAGAGAGGTCGGCAGGCGGGCCCCGGCCCGCCTGCCCTGCCGTTGCGTGAAGCGCTGGGCCGc                                                                                                               <  2:360855/150‑1 (MQ=255)
                                  ccTTGCATAGGGCTACTCCTGGATTTGGGCAACAGCAACCCGGCGCGGCGGCACTGCATGGCCACCGGCGTGCGGGTTGCATCGAGAGAGGTCGGCAGGCGGGCCCCGGCCCGCCTGCCCTGCCGTTGCGTGAAGCGCTGGGCCGCTAGc                                                                                                           >  2:399960/1‑150 (MQ=255)
                                    ttGCATAGGGCTACTCCTGGAGTTGGGCAACAGCAACCCGGCGCGGCGGCACTGCATGGCCACCGGCGTGCGGGTTGCATCGAGAGAGGTCGGCAGGCGGGCCCCGGCCCGCCTGCCCTGCCGTTGCGTGAAGCGCTGGGCCGCTAGCgg                                                                                                         >  2:21229/1‑150 (MQ=255)
                                      gCATAGGGCTACTCCTGGATTTGGGCAACAGCAACCCGGCGCGGCGGCACTGCATGGCCACCGGCGTGCGGGTTGCATCGAGAGAGGTCGGCAGGCGGGCCCCGGCCCGCCTGCCCTGCCGTTGCGTGAAGCGCTGGGCCGCTAGCGGtt                                                                                                       <  2:286673/150‑1 (MQ=255)
                                        aTAGGGCTACTCCTGGATTTGGGCAACAGCAACCCGGCGCGGCGGCACTGCATGGCCACCGGCGTGCGGGTTGCATCGAGAGAGGTCGGCAGGCGGGCCCCGGCCCGcct                                                                                                                                             <  1:512245/110‑1 (MQ=255)
                                        aTAGGGCTACTCCTGGATTTGGGCAACAGCAACCCGGCGCGGCGGCACTGCATGGCCACCGGCGTGCGGGTTGCATCGAGAGAGGTCGGCAGGCGGGCCCCGGCCCGcct                                                                                                                                             >  2:512245/1‑110 (MQ=255)
                                         tAGGGCTACTCCTGGATTTGGGCAACAGCAACCCGGCGCGGCGGCACTGCATGGCCACCGGCGTGCGGGTTGCATCGAGAGAGGTCGGCAGGCGGGCCCCCGCCCGCCTCCCCCGCCGTTGCGTGAAGCGCTGGGCCGCTAGCGGTTGAc                                                                                                    <  1:25923/150‑1 (MQ=255)
                                            ggCTACCCCTGGATTTGGCCAACAGCCACCCGGCGCGGCGGCACTGCATGGACACCGGCGTGCGGGTTGTATGATTTGATGGCGGCAGGCGGGCCCCGGCCCGCCTGCCCTGCCGTTGCGTGAAGCGCTGGGCCGCTAGCGGTTGACCAg                                                                                                 <  2:1189309/150‑1 (MQ=255)
                                                   ccTGGATTTGGGCAACAGCAACCCGGCGCGGCGGCACTGCATGGCCACCGGCGTGCGGGTTGCATCGAGAGAGGTCGGCAGGCGGGCCCCGGCCCGCCTGCCCTGCCGTTGCGTGAAGCGCTGGGCCGCTAGCGGTTGACCAGCTTGGcc                                                                                          <  1:287204/150‑1 (MQ=255)
                                                     tGGATTTGGGCAACAGCAACCCGGCGCGGCGGCACTGCATGGCCACCGGCGTGCGGGTTGCATCGAGAGAGGTCGGCAGGCGGGCCCCGGCCCGCCTGCCCTGCCGTTGCGTGAAGCGCTGGGCCGCTAGCGGTTGACCAGCTTGGCCgg                                                                                        >  1:1182800/1‑150 (MQ=255)
                                                          ttGGGCAACAGCAACCCGGCGCGGCGGCACTGCATGGCCACCGGCGTGCGGGTTGCATCGAGAGAGGTCGGCAGGCGGGCCCCGGCCCGCCTGCCCTGCCGTTGCGTGAAGCGCTGGGCCGCTAGCGGTTGACCAGCTTGGCCGGCAAgg                                                                                   <  1:399960/150‑1 (MQ=255)
                                                          ttGGGCAACAGCAACCCGGCGCGGCGGCACTGCATGGCCACCGGCGTGCGGGTTGCATCGAGAGAGGTCGGCAGGCGGGCCCCGGCCCGCCTGCCCTGCCGTTGCGTGAAGCGCTGGGCCGCTAGCGGTTGACCAGCTTGGCCGGCAAgg                                                                                   <  1:484366/150‑1 (MQ=255)
                                                          ttGGGCAACAGCAACCCGGCGCGGCGGCACTGCATGGCCACCGGCGTGCGGGTTGCATCGAGAGAGGTCGGCAGGCGGGCCCCGGCCCGCCTGCCCTGCCGTTGCGTGAAGCGCTGGGCCGCTAGCGGTTGACCAGCTTGGCCGGCAAgg                                                                                   <  2:218855/150‑1 (MQ=255)
                                                             ggCAACAGCAACCCGGCGCGGCGGCACTGCATGGCCACCGGCGTGCGGGTTGCATCGAGAGAGGTCGGCAGGCGGGCCCCGGCCCGCCTGCCCTGCCGTTGCGTGAAGCGCTGGGCCGCTAGCGGTTGACCAGCTTGGcc                                                                                          >  2:403388/1‑140 (MQ=255)
                                                             ggCAACAGCAACCCGGCGCGGCGGCACTGCATGGCCACCGGCGTGCGGGTTGCATCGAGAGAGGTCGGCAGGCGGGCCCCGGCCCGCCTGCCCTGCCGTTGCGTGAAGCGCTGGGCCGCTAGCGGTTGACCAGCTTGGcc                                                                                          <  1:403388/140‑1 (MQ=255)
                                                                aaCAGCAACCCGGCGCGGCGGCACTGCATGGCCACCGGCGTGCGGGTTGCATCGAGAGAGGTCGGCAGGCGGGCCCCGGCCCGCCTGCCCTGCCGTTGCGTGAAGCGCTGGGCCGCTAGCGGTTGACCAGCTTGGCCGGCAAGGCGGTGa                                                                             >  1:601486/1‑150 (MQ=255)
                                                                aaCAGCAACCCGGCGCGGCGGCACTGCATGGCCACCGGCGTGCGGGTTGCATCGAGAGAGGTCGGCAGGCGGGCCCCGGCCCGCCTGCCCTGCCGTTGCGTGAAGCGCTGGGCCGCTAGCGGTTGACCAGCTTGGCCGGCAAGGCGATGa                                                                             <  1:431422/150‑1 (MQ=255)
                                                                         ccGGCGCGGCGGCACTGCATGGCCACCGGCGTGCGGGTTGCATCGAGAGAGGTCGGCAGGCGGGCCCCGGCCCGCCTGCCCTGCCGTTGCGTGAAGCGCTGGGCCGCTAGCGGTTGACCAGCTTGGCCGGCAAGGCGATGACCAGGAagc                                                                    >  1:741527/1‑150 (MQ=255)
                                                                           ggcgcggcgGCACTGCATGGCCACCGGCGTGCGGGTTGCATCGAGAGAGGTCGGCAGGCGGGCCCCGGCCCGCCTGCCCTGCCGTTGCGTGAAGCGCTGGGCCGCTAGCGGTTGACCAGCTTGGCCGGCAAGGCGATGACCAGGAagcag                                                                  <  1:367291/150‑1 (MQ=255)
                                                                           ggcgcggcgGCACTGCATGGCCACCGGCGTGCGGGTTGCATCGAGAGAGGTCGGCAGGCGGGCCCCGGCCCGCCTGCCCTGCCGTTGCGTGAAGCGCTGGGCCGCTAGCGGTTGACCAGCTTGGCCGGCAAGGCGATGACCAGGAagcag                                                                  <  2:590908/150‑1 (MQ=255)
                                                                           ggcgcggcgGCACTGCATGGCCACCGGCGTGCGGGTTGCATCGAGAGAGGTCGGCAGGCGGGCCCCGGCCCGCCTGCCCTGCCGTTGCGTGAAGCGCTGGGCCGCTAGCGGTTGACCAGCTTGGCCGGCAAGGCGATGACCAGGAagcag                                                                  <  2:741527/150‑1 (MQ=255)
                                                                                                     gCGTGCGGGTTGCATCGAGAGAGGTCGGCAGGCGGGCCCCGGCCCGCCTGCCCTGCCGTTGCGTGAAGCGCTGGGCCGCTAGCGGTTGACCAGCTTGGCCGGCAAGGCGATGACCAGGAAGCAGCTGAGGATCAGGCAGCCGGCAAacac                                        >  1:281945/1‑150 (MQ=255)
                                                                                                           gggTTGCATCGAGAGAGGTCGGCAGGCGGGCCCCGGCCCGCCTGCCCTGCCGTTGCGTGAAGCGCTGGGCCGCTAGCGGTTGACCAGCTTGGCCGGCAAGGCGATGACCAGGAAGCAGCTGAGGATCAGGCAGCCGGCAAACACCCAcag                                  >  1:609489/1‑150 (MQ=255)
                                                                                                           gggTTGCATCGAGAGAGGTCGGCAGGCGGGCCCCGGCCCGCCTGCCCTGCCGTTGCGTGAAGCGCTGGGCCGCTAGCGGTTGACCAGCTTGGCCGGCAAGGCGATGACCAGGAAGCAGCTGAGGATCAGGCAGCCGGCAAACACCCAcag                                  <  1:1100046/150‑1 (MQ=255)
                                                                                                                 cATCGAGAGAGGTCGGCAGGCGGGCCCCGGCCCGCCTGCCCTGCCGTTGCGTGAAGCGCTGGGCCGCTAGCGGTTGACCAGCTTGGCCGGCAAGGCGATGACCAGGAAGCAGCTGAGGATCAGGCAGCCGGCAAACACCCAcagcgcagc                            >  1:70002/1‑150 (MQ=255)
                                                                                                                   tCGAGAGAGGTCGGCAGGCGGGCCCCGGCCCGCCTGCCCTGCCGTTGCGTGAAGCGCTGGGCCGCTAGCGGTTGACCAGCTTGGCCGGCAAGGCGATGACCAGGAAGCAGCTGa                                                              >  1:75248/1‑114 (MQ=255)
                                                                                                                   tCGAGAGAGGTCGGCAGGCGGGCCCCGGCCCGCCTGCCCTGCCGTTGCGTGAAGCGCTGGGCCGCTAGCGGTTGACCAGCTTGGCCGGCAAGGCGATGACCAGGAAGCAGCTGa                                                              <  2:75248/114‑1 (MQ=255)
                                                                                                                       gagagGTCGGCAGGCGGGCCCCGGCCCGCCTGCCCTGCCGTTGCGTGAAGCGCTGGGCCGCTAGCGGTTGACCAGCTTGGCCGGCAAGGCGATGACCAGGAAGCAGCTGAGGATCAGGCAGCCGGCAAACACCCACAGCGCAGCGTGCGa                      <  2:281945/150‑1 (MQ=255)
                                                                                                                               ggCAGGCGGGCCCCGGCCCGCCTGCCCTGCCGTTGCGTGAAGCGCTGGGCCGCTAGCGGTTGACCAGCTTGGCCGGCAAGGCGATGACCAGGAagcag                                                                  <  2:195398/98‑1 (MQ=255)
                                                                                                                               ggCAGGCGGGCCCCGGCCCGCCTGCCCTGCCGTTGCGTGAAGCGCTGGGCCGCTAGCGGTTGACCAGCTTGGCCGGCAAGGCGATGACCAGGAagcag                                                                  >  1:195398/1‑98 (MQ=255)
                                                                                                                                   ggCGGGCCGGGGCCCGCCTGCCCTGCCGTTGCGTGAAGCGCTGGGCCGCTAGCGGTTGACCAGCTTGGCCGGCAAGGCGATGACCAGGAAGCAGCTGAGGATCAGGCAGCCGGCAAACACCCACAGCGCAGCGTGCGAGGTGCCGGTGAg          >  2:745330/1‑150 (MQ=255)
                                                                                                                                         ccccGGCCCGCCTGCCCTGCCGTTGCGTGAAGCGCTGGGCCGCTAGCGGTTGACCAGCTTGGCCGGCAAGGCGATGACCAGGAAGCAGCTGAGGATCAGGCAGCCGGCAAACACCCACAGCGCAGCGTGCGAGGTGCCGGTGAGGTCGGt    >  2:877896/1‑150 (MQ=255)
                                                                                                                                           ccGGCCCGCCTGCCCTGCCGTTGCGTGAAGCGCTGGGCCGCTAGCGGTTGACCAGCTTGGCCGGCAAGGCGATGACCAGGAAGCAGCTGAGGATCAGGCAGCCGGCAAACACCCACAGCGCAGCGTGCGAGGTGCCGGTGAGGTCGGTGa  <  2:70002/150‑1 (MQ=255)
                                                                                                                                            |                                                                                                                                                    
GCGTGCAGACGAAGAAAAACGCGGCGCAGTGAAACCTTGCATAGGGCTACTCCTGGATTTGGGCAACAGCAACCCGGCGCGGCGGCACTGCATGGCCACCGGCGTGCGGGTTGCATCGAGAGAGGTCGGCAGGCGGGCCCCGGCCCGCCTGCCCTGCCGTTGCGTGAAGCGCTGGGCCGCTAGCGGTTGACCAGCTTGGCCGGCAAGGCGATGACCAGGAAGCAGCTGAGGATCAGGCAGCCGGCAAACACCCACAGCGCAGCGTGCGAGGTGCCGGTGAGGTCGGTGA  >  NC_002947/4442533‑4442821

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑