| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 4,456,509 | T→C | 7.7% | M174V (ATG→GTG) | PP_3950 ← | methyl‑accepting chemotaxis transducer |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 4,456,509 | 0 | T | C | 7.7% | 151.2 / 3.0 | 52 | M174V (ATG→GTG) | PP_3950 | methyl‑accepting chemotaxis transducer |
| Reads supporting (aligned to +/- strand): ref base T (27/21); new base C (2/2); total (29/23) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.15e-01 | |||||||||||
GCCAGGGTCGAAGCGCGATTGCGCCATGAACAGGTAGTTGTCGCCCGACTC‑GGGGTAAATGTGCCCTGCCTCGCGCTGGATCAGGTCACCCAGCACATCGTTGGGCACCCGCCCACACAGACAACCCAGCACTTTG‑CCCTCGTGCATGAGGGGCTGGTAGAACATCAACGTGACTTCATCGTGGAAGCGCGAGGAAGACGGGCCGATCTGCAAGGTCAGCGAGTCGCTGTAGGGCCCGTGCAGAAACGGTGCTTTCAGCCCCTGCGCCAGAGCTGCCAAATACTCGATACGTGGCG > NC_002947/4456363‑4456658 | gCCAGGGTCGAAGCGCGATTGCGCCATGAACAGGTAGTTGTCGCCCGTCTCAGATGATGATGT‑CCCGGCCTCTCGCTGGATCAGGTCACCCAGCACATCGTTGGGCACCCGCCCACACAGACAACCCAGCACTTTG‑CCCTCGTGCATGAg < 1:142139/150‑1 (MQ=255) ccAGGGTCGAAGCGCGATTGCGCCATGAACAGGTAGTTGTCGCCCGACTC‑GGGGTAAATGTGCCCTGCCTCGCGCTGGATCAGGTCACCCAGCACATCGTTGGGCACCCGCCCACACAGACAACCCAGCACTTTG‑CCCTCGTGCATGAgg > 2:1059159/1‑150 (MQ=255) aGGGTCGAAGCGCGATTGCGCCATGAACAGGTAGTTGTAGCCCGACTC‑GGGGTAAATGTGCCCTGCCTCGCGCTGGATCAGGTCACCCAGCACATCGTTGGGCACCCGCCCACACAGACAACCCAGCACTTTG‑CCCTCGTGCATGAgggg > 2:42544/1‑150 (MQ=255) tGCGCCATGAACAGGTAGTTGTCGCCCGACTC‑GGGGTAAATGTGCCCTGCCTCGCGCTGGATCAGGTCACCCAGCACATCGTTGGGCACCCGCCCACACAGACAACCCAGCACTTTG‑CCCTCGTGCATGAGGGGCTGGTAGAACATCAAc < 1:1059159/150‑1 (MQ=255) aCAGGTAGTTGTCGCCCGACTC‑GGGGTAAATGTGCCCTGCCTCGCGCTGGATCAGGTCACCCAGCACATCGTTGGGCACCCGCCCACACAGACAACCCAGCACTTTG‑CCCTCGTGCATGAGGGGCTGGTAGAACATCAAc > 2:575919/1‑140 (MQ=255) aCAGGTAGTTGTCGCCCGACTC‑GGGGTAAATGTGCCCTGCCTCGCGCTGGATCAGGTCACCCAGCACATCGTTGGGCACCCGCCCACACAGACAACCCAGCACTTTG‑CCCTCGTGCATGAGGGGCTGGTAGAACATCAAc < 1:575919/140‑1 (MQ=255) cAGGTAGTTGTCGCCCGACTC‑GGGGTAAATGTGCCCTGCCTCGCGCTGAATCAGGTCACCCAGCACATCGTTGGGCACCCGCCCACACAGACAACCCAGCACTTTGCCCCTCATGCACGAgggcaaagt < 2:832000/129‑7 (MQ=255) cAGGTAGTTGTCGCCCGACTC‑GGGGTAAATGTGCCCTGCCTCGCGCTGAATCAGGTCACCCAGCACATCGTTGGGCACCCGCCCACACAGACAACCCAGCACTTTGCCCCTCATGCACGAgggcaaagt > 1:832000/1‑123 (MQ=255) gTAGTTGTCGCCCGACTC‑GGGGTAAATGTGCCCTGCCTCGCGCTGGATCAGGTCACCCAGCACATCGTTGGGCACCCGCCCACACAGACAACCCAGCACTTTG‑CCCTCGTGCATGAGGGGCTGGTAGAACATCAACGTGACTTCATCGTg > 1:909731/1‑150 (MQ=255) gTTGTCGCCCGACTC‑GGGGTAAATGTGCCCTGCCTCGCGCTGGATCAGGTCACCCAGCACATCGTTGGGCACCCGCCCACACAGACAACCCAGCACTTTG‑CCCTCGTGCATGAGGGGCTGGTAGAACATCAACGTGACTTCATCGTGGaa < 2:325886/150‑1 (MQ=255) gTTGTCGCCCGACTC‑GGGGTAAATGTGCCCTGCCTCGCGCTGGATCAGGTCACCCAGCACATCGTTGGGCACCCGCCCACACAGACAACCCAGCACTTTG‑CCCTCGTGCATGAGGGGCTGGTAGAACATCAACGTGACTTCATCGTGGaa < 1:981409/150‑1 (MQ=255) cGACTC‑GGGGTAAATGTGCCCTGCCTCGCGCTGGATCAGGTCACCCAGCACATCGTTGGGCACCCGCCCACACAGACAACCCAGCACTTTG‑CCCTCGTGCATGAGGGGCTGGTAGAACATCAACGTGACTTCATCGTGGAAGCGCGAGGa < 1:606392/150‑1 (MQ=255) tC‑GGGGTAAATGTGCCCTGCCTCGCGCTGGATCAGGTCACCCAGCACATCGTTGGGCACCCGCCCACACAGACAACCCAGCACTTTG‑CCCTCGTGCATGAgg > 2:719695/1‑102 (MQ=255) tC‑GGGGTAAATGTGCCCTGCCTCGCGCTGGATCAGGTCACCCAGCACATCGTTGGGCACCCGCCCACACAGACAACCCAGCACTTTG‑CCCTCGTGCATGAgg < 1:719695/102‑1 (MQ=255) tC‑GGGGTAAATGTGCCCTGCCTCGCGCTGGATCAGGTCACCCAGCACATCGTTGGGCACCCGCCCACACAGACAACCCAGCACTTTG‑CCCTCGTGCATGAGGGGCTGGTAGAACATCAACGTGACTTCATCGTGGAAGCGCGAGGAAGAc < 1:42544/150‑1 (MQ=255) gggTAAATGTGCCCTGCCTCGCGCTGGATCAGGTCACCCAGCACATCGTTGGGCACCCGCCCACACAGACAACCCAGCACTTTG‑CCCTCGTGCATGAGGGGCTGGTAGAACATCAACGTGACTTCATCGTGGAAGCGCGAGGAAGACggg > 2:1071055/1‑150 (MQ=255) gTAAATGTGCCCTGCCTCGCGCTGGATCAGGTCACCCAGCACATCGTTGGGCACCCGCCCACACAGACAACCCAGCACTTTG‑CCCTCGTGCATGAGGGGCTg < 1:1075323/102‑1 (MQ=255) gTAAATGTGCCCTGCCTCGCGCTGGATCAGGTCACCCAGCACATCGTTGGGCACCCGCCCACACAGACAACCCAGCACTTTG‑CCCTCGTGCATGAGGGGCTg > 2:1075323/1‑102 (MQ=255) gTAAATGTGCCCTGCCTCGCGCTGGATCAGGTCACCCAGCACATCGTTGGGCACCCGCCCACACAGACAACCCAGCACTTTG‑CCCTCGTGCATGAGGGGCTGGTAGAACATCAACGTGACTTCATCGTGGAAGCGCGAGGAAGACGGGcc > 1:446189/1‑150 (MQ=255) aaTGTGCCCTGCCTCGCGCTGGATCAGGTCACCCAGCACATCGTTGGGCACCCGCCCACACAGACAACCCAGCACTTTG‑CCCTCGTGCATGAGGGGCTGGTAGAACATCAACGTGACTTCATCGTGGAAGCGCGAGGAAGACGGGCCGAt < 1:375557/150‑1 (MQ=255) cccTGCCTCGCGCTGGATCAGGTCACCCAGCACATCGTTGGGCACCCGCCCACACAGACAACCCAGCACTTTG‑CCCTCGTGCATGAGGGGCTGGTAGAACATCAACGt > 1:517802/1‑108 (MQ=255) cccTGCCTCGCGCTGGATCAGGTCACCCAGCACATCGTTGGGCACCCGCCCACACAGACAACCCAGCACTTTG‑CCCTCGTGCATGAGGGGCTGGTAGAACATCAACGt < 2:517802/108‑1 (MQ=255) ccTCGCGCTGGATCAGGTCACCCAGCACATCGTTGGGCACCCGCCCACACAGACAACCCAGCACTTTG‑CCCTCGTGCATGAGGGGCTGGTAGAACATCAACGTGACTTCATCGTGGAAGCGCGAGGAAGACGGGCCGATCTGCAAGGTCa > 2:26837/1‑150 (MQ=255) tCGCGCTGGATCAGGTCACCCAGCACATCGTTGGGCACCCGCCCACACAGACAACCCAGCACTTTG‑CCCTCGTGCATGAGGGGCTGGTAGAACATCAACGTGACTTCATCGTGGAAGCGCGAGGAAGACGGGCCGATCTGCAAGGTCAGc > 2:176853/1‑150 (MQ=255) gcTGGATCAGGTCACCCAGCACATCGTTGGGCACCCGCCCACACAGACAACCCAGCACTTTG‑CCCTCGTGCATGAGGGGCTGGTAGAACATCAACGTGACTTCATCGTGGAAGCGCGAGGAAGACGGGCCGATCTGCAAGGTCAGCGAGt < 1:1071055/150‑1 (MQ=255) tGGATCAGGTCACCCAGCACATCGTTGGGCACCCGCCCACACAGACAACCCAGCACTTTG‑CCCTCGTGCATGAGGGGCTGGTAGAACATCAACGTGACTTCATCGTGGAAGCGCGAGGAAGACGGGCCGATCTGCAAGGTCa > 1:724736/1‑142 (MQ=255) tGGATCAGGTCACCCAGCACATCGTTGGGCACCCGCCCACACAGACAACCCAGCACTTTG‑CCCTCGTGCATGAGGGGCTGGTAGAACATCAACGTGACTTCATCGTGGAAGCGCGAGGAAGACGGGCCGATCTGCAAGGTCa < 2:724736/142‑1 (MQ=255) aTCAGGTCACCCAGCACATCGTTGGGCACCCGCCCACACAGACAACCCAGCACTTTG‑CCCTCGTGCATGAGGGGCTGGTAGAACATCAACGTGACTTCATCGTGGAAGCGCGAGGAAGACGGGCCGATCTGCAAGGTCAGCGAGTCGCTg > 2:90164/1‑150 (MQ=255) tCAGGTCACCCAGCACATCGTTGGGCACCCGCCCACACAGACAACCCAGCACTTTG‑CCCTCGTGCATGAGGGGCTGGTAGAACATCAACGTGACTTCATCGTGGAAGCGCGAGGAAGACGGGCCGATCt > 1:191706/1‑129 (MQ=255) tCAGGTCACCCAGCACATCGTTGGGCACCCGCCCACACAGACAACCCAGCACTTTG‑CCCTCGTGCATGAGGGGCTGGTAGAACATCAACGTGACTTCATCGTGGAAGCGCGAGGAAGACGGGCCGATCt < 2:191706/129‑1 (MQ=255) cAGGTCACCCAGCACATCGTTGGGCACCCGCCCACACAGACAACCCAGCACTTTG‑CCCTCGTGCATGAGGGGCTGGTAGAACATCAACGTGACTTCATCGTGGAAGCGCGAGGAAGACGGGCCGATCTGCAAGGTCAGCGAGTCGCTgca < 1:90164/150‑3 (MQ=255) aGGTCACCCAGCACATCGTTGGGCACCCGCCCACACAGACAACCCAGCACTTTG‑CCCTCGTGCATGAGGGGCTGGTAGAACATCAACGTGACTTCATCGTGGAAGCGCGAGGAAGACGGGCCGATCTGCAAGGTCAGCGAGTCGCTGTAg < 2:446189/150‑1 (MQ=255) aGGTCACCCAGCACATCGTTGGGCACCCGCCCACACAGACAACCCAGCACTTTG‑CCCTCGTGCATGAGGGGCTGGTAGAACATCAACGTGACTTCATCGTGGAAGCGCGAGGAAGACGGGCCGATCTGCAAGGTCAGCGAGTCGCTGTAg > 2:342901/1‑150 (MQ=255) ccAGCACATCGTTGGGCACCCGCCCACACAGACAACCCAGCACTTTG‑CCCTCGTGCATGAGGGGCt > 2:461415/1‑66 (MQ=255) ccAGCACATCGTTGGGCACCCGCCCACACAGACAACCCAGCACTTTG‑CCCTCGTGCATGAGGGGCt < 1:461415/66‑1 (MQ=255) aTCGTTGGGCACCCGCCCACACAGACAACCCAGCACTTTG‑CCCTCGTGCATGAGGGGCTGGTAGAACATCAACGTGACTTCATCGTGGAAGCGCGAGGAAGACGGGCCGATCTGCAAGGTCAGCGAGTCGCTGTAGGGCCCGTGCAGaaa > 2:244059/1‑150 (MQ=255) gTTGGGCACCCGCCCACACAGACAACCCAGCACTTTG‑CCCTCGTGCATGAGGGGCTGGTAGAACATCAACGTGACTTCATCGTGGAAGCGCGAGGAAGACGGGCCGATCTGCAAGGTCAGCGAGTCGCTGTAGGGCCCGTGCAGAAACgg > 1:1198650/1‑150 (MQ=255) gTTGGGCACCCGCCCACACAGACAACCCAGCACTCTG‑CCCTCGTGCATGAGGGGCTGGTAGAACATCAACGTGACATCATCGTGGAAGCGCGAGAACGACGGTCCGATCTGCAAGGTCACCGAGCCGCTGTAAGGCCCAGACAGAAACgg > 2:327639/1‑150 (MQ=255) gCACCCGCCCACACAGACAACCCAGCACTTTG‑CCCTCGTGCATGAGGGGCTGGTAGAACATCAACGTGACTTCATCGTGGAAGCGCGAGGAAGACGGGCCGATCTGCAAGGTCAGCGAGTCGCTGTAGGGCCCGTGCAGAAACGGTGCtt > 2:561394/1‑150 (MQ=255) ccACACAGACAACCCAGCACTTTG‑CCCTCGTGCATGAGGGGCTGGTAGAACATCAACGTGACTTCATCGTGGAAGCGCGAGGAAGACGGGCCGATCTGCAAGGTCAGCGAGTCGCTGTAGGGCCCGTGCAGAAACGGTGCTTTCAGcccc > 2:770874/1‑150 (MQ=255) aGACAACCCAGCACTTTG‑CCCTCGTGCATGAGGGGCTGGTAGAACATCAACGTGACTTCATCGTGGAAGCGCGAGGAAGACGGGCCGATCTGCAAGGTCAGCGAGTCGCTGTAGGGCCCGTGCAGAAACGGTGCTTTCAGCCCCTGCGcc > 2:607189/1‑150 (MQ=255) gACAACCCAGCACTTTG‑CCCTCGTGCATGAGGGGCTGGTAGAACATCAACGTGACTTCATCGTGGAAGCGCGAGGAAGACGGGCCGATCTGCAAGGTCAGCGAGTCGCTGTAGGGCCCGTGCAGAAACGGTGCTTTCAGCCCCTGCGCCa < 1:176853/150‑1 (MQ=255) cAACCCAGCACTTTG‑CCCTCGTGCATGAGGGGCTGGTAGAACATCAACGTGACTTCATCGTGGAAGCGCGAGGAAGACGGGCCGATCTGCAAGGTCAGCGAGTCGCTGTAGGGCCCGTGCAGAAACGGTGCTTTCAGCCCCTGCGCCaga < 1:244059/150‑1 (MQ=255) cAGCACTTTG‑CCCTCGTGCATGAGGGGCTGGTAGAACATCAACGTGACTTCATCGTGGAAGCGCGAGGAAGACGGGCCGATCTGCAAGGTCAGCGAGTCGCTGTAGGGCCCGTGCAGAAACGGTGCTTTCAGCCCCTGCGCCAGAGCTGc > 2:789820/1‑150 (MQ=255) cAGCACTTTG‑CCCTCGTGCATGAGGGGCTGGTAGAACATCAACGTGACTTCATCGTGGAAGCGCGAGGAAGACGGGCCGATCTGCAAGGTCAGCGAGTCGCTGTAGGGCCCGTGCAGAAACGGTGCTTTCAGCCCCTGCGCCAGAGCTGc > 2:201034/1‑150 (MQ=255) agccccTCATGCACGAGGGGCTGGTAGAACATCAACGTGACTTCATCGTGGAAGCGCGAGGAAGACGGGCCGATCTGCAAGGTCAGCGAGTCGCTGTAGGGCCCGTGCAGAAACGGTGCTTTc < 2:1107870/120‑1 (MQ=255) agccccTCATGCACGAGGGGCTGGTAGAACATCAACGTGACTTCATCGTGGAAGCGCGAGGAAGACGGGCCGATCTGCAAGGTCAGCGAGTCGCTGTAGGGCCCGTGCAGAAACGGTGCTTTc > 1:1107870/4‑123 (MQ=255) g‑CCCTCGTGCATGAGGGGCTGGTAGAACATCAACGTGACTTCATCGTGGAAGCGCGAGGAAGACGGGCCGATCTGCAAGGTCAGCGAGTCGCTGTAGGGCCCGTGCAGAAACGGTGCTTTCAGCCCCTGCGCCAGAGCTGCCAAATACTc < 1:607189/150‑1 (MQ=255) ccTCGTGCATGAGGGGCTGGTAGAACATCAACGTGACTTCATCGTGGAAGCGCGAGGAAGACGGGCCGATCTGCAAGGTCAGCGAGTCGCTGTAGGGCCCGTGCAGAAACGGTGCTTTCAGCCCCTGCGCCAGAGCTGCCAAATACTCGa > 2:569017/1‑150 (MQ=255) ccTCGTGCATGAGGGGCTGGTAGAACATCAACGTGACTTCATCGTGGAAGCGCGAGGAAGACGGGCCGATCTGCAAGGTCAGCGAGTCGCTGTAGGGCCCGTGCAGAAACGGTGCTTTCAGCCCCTGCGCCAGAGCTGCCAAATACTCGa > 2:279194/1‑150 (MQ=255) ccTCGTGCATGAGGGGCTGGTAGAACATCAACGTGACTTCATCGTGGAAGCGCGAGGAAGACGGGCCGATCTGCAAGGTCAGCGAGTCGCTGTAGGGCCCGTGCAGAAACGGTGCTTTCAGCCCCTGCGCCAGAGCTGCCAAATACTCGa > 1:1003298/1‑150 (MQ=255) ccTCGTGCATGAGGGGCTGGTAGAACATCAACGTGACTTCATCGTGGAAGCGCGAGGAAGACGGGCCGATCTGCAAGGTCAGCGAGTCGCTGTAGGGCCCGTGCAGAAACGGTGCTTTCAGCCCCTGCGCCAGAGCTGCCAAATACTCGa < 1:721408/150‑1 (MQ=255) tGAGGGGCTGGTAGAACATCAACGTGACTTCATCGTGGAAGCGCGAGGAAGACGGGCCGATCTGCAAGGTCAGCGAGTCGCTGTAGGGCCCGTGCAGAAACGGTGCTTTCAGCCCCTGCGCCAGAGCTGCCAAATACTCGATACGTGGCg > 2:397953/1‑150 (MQ=255) | GCCAGGGTCGAAGCGCGATTGCGCCATGAACAGGTAGTTGTCGCCCGACTC‑GGGGTAAATGTGCCCTGCCTCGCGCTGGATCAGGTCACCCAGCACATCGTTGGGCACCCGCCCACACAGACAACCCAGCACTTTG‑CCCTCGTGCATGAGGGGCTGGTAGAACATCAACGTGACTTCATCGTGGAAGCGCGAGGAAGACGGGCCGATCTGCAAGGTCAGCGAGTCGCTGTAGGGCCCGTGCAGAAACGGTGCTTTCAGCCCCTGCGCCAGAGCTGCCAAATACTCGATACGTGGCG > NC_002947/4456363‑4456658 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |