| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 4,604,250 | C→G | 12.2% | P12A (CCG→GCG) ‡ | PP_4076 → | hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 4,604,250 | 0 | C | G | 12.2% | 118.2 / 8.7 | 41 | P12A (CCG→GCG) ‡ | PP_4076 | hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base C (20/16); new base G (3/2); total (23/18) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.41e-01 | |||||||||||
ACGTGGCGGGTGATCCGGGCTGGTACCAGGTCTCGCTCGCCGTGCGCCCGCACTTCAAGTACATGGGCGCGAACTTCGAAATTTCCCTGGTAGGCCGGCTCGACACGCAATGAGTGGTTTCTTCGATCGCTTGATGGCGGACCCGTCCGCCATCCGGGAGGCGTCTCGCCAGGAAAGCGCTTCGCACAAGTTCGCGGCCATCAAGTGCCACCTCGAAACGTTGCTGAACGCTCGCCAAGGTTGCTCCCAGAGCAGCCCCGAGCTGGGCCTGCGCGACTTCAACGGGCAT > NC_002947/4604108‑4604396 | aCGTGGCGGGTGATCCGGGCTGGTACCAGGTCTCGCTCGCCGTGCGCCCGCACTTCAAGTACATGGGCGCGAACTTCGAAATTTCCCTGGTAGGCCGGCTCGACACGCAATGAGTGGTTTCTTCGATCGCTGGATGGCGGACGGGTCCGc > 1:260369/1‑150 (MQ=255) tGATCCGGGCTGGTACCAGGTCTCGCTCGCCGTGCGCCCGCACTTCAAGTACATGGGCGCGAACTTCGAAATTTCCCTGGTAGGCCGGCTCGACACGCAATGAGTGGTTTCTTCGATCGCTTGATGGCGGACCCGTCCGCCATCCGGGAg < 1:307144/150‑1 (MQ=255) gTACCAGGTCTCGCTCGCCGTGCGCCCGCACTTCAAGTACATGGGCGCGAACTTCGAAATTTCCCTGGTAGGCCGGCTCGACACGCAATGAGTGGTTTCTTCGATCGCTTGATGGCGGACCCGTCCGCCATCCGGGAGGCGTCTCGCCAg > 1:911432/1‑150 (MQ=255) ggTCTCGCTCGCCGTGCGCCCGCACTTCAAGTACATGGGCGCGAACTTCGAAATTTCCCTGGTAGGCCGGCTCGACACGCAATGAGTGGTTTCTTCGATCGCTTGATGGCGGACCCGTCCGCCATCCGGGAGGCGTCTCGCCAGGAAAgc > 1:623741/1‑150 (MQ=255) ctcgcCGTGCGCCCGCACTTCAAGTACATGGGCGCGAACTTCGAAATTTCCCTGGTAGGCCGGCTCGACACGCAATGAGTGGTTTCTTCGATCGCTTGATGGCGGACCCGTCCGCCATCCGGGAGGCGTCTCGCCAGGAAAGCGCTTCGc > 2:198894/1‑150 (MQ=255) ttCAAGTACATGGGCGCGAACTTCGAAATTTCCCTGGTAGGCCGGCTCGACACGCAATGAGTGGTTTCTTCGATCGCTTGATGGCGGACCCGTCCGCCATCCGGGAGGCGTCTCGCCAGGAAAGCGCTTCGCACAAGTTCGCGGCCATCa > 1:525312/1‑150 (MQ=255) cAAGTACATGGGCGCGAACTTCGAAATTTCCCTGGTAGGCCGGCTCGACACGCAATGAGTGGTTTCTTCGATCGCTTGATGGCGGACCCGTc > 1:115253/1‑92 (MQ=255) cAAGTACATGGGCGCGAACTTCGAAATTTCCCTGGTAGGCCGGCTCGACACGCAATGAGTGGTTTCTTCGATCGCTTGATGGCGGACCCGTc < 2:115253/92‑1 (MQ=255) aaGTACATGGGCGCGAACTTCGAAATTTCCCTGGTAGGCCGGCTCGACACGCAATGAGTGGTTTCTTCGATCGCTTGATGGCGGACCCGTCCGCCATCCGGGAGGCGTCTCGCCAGGAAAGCGCTTCGCACAAGTTCGCGGCCATCAAGt < 1:198894/150‑1 (MQ=255) aTGGGCGCGAACTTCGAAATTTCCCTGGTAGGCCGGCTCGACACGCAATGAGTGGTTTCTTCGATCGCTGGATGGCGGACGGGTCCGCCATc > 2:493931/1‑92 (MQ=255) aTGGGCGCGAACTTCGAAATTTCCCTGGTAGGCCGGCTCGACACGCAATGAGTGGTTTCTTCGATCGCTGGATGGCGGACGGGTCCGCCATc < 1:493931/92‑1 (MQ=255) gcgcGAACTTCGAAATTTCCCTGGTAGGCCGGCTCGACACGCAATGAGTGGTTTCTTCGATCGCTTGATGGCGGACCCGTCCGCCATCCGGGAGGCGTCTCGCCAGGAAAGCGCTTCGCACAAGTTCGCGGCCATCAAGTGCCACCTCGa > 1:13817/1‑150 (MQ=255) gAACTTCGAAATTTCCCTGGTAGGCCGGCTCGACACGCAATGAGTGGTTTCTTCGATCGCTTGATGGCGGACCCGTCCGCCATCCGGGAGGCGTCTCGCCAGGAAAGCGCTTCGCACAAGTTCGCGGCCATCAAGTGCCACCTCGAAACg > 1:317159/1‑150 (MQ=255) ttCGAAATTTCCCTGGTAGGCCGGCTCGACACGCAATGAGTGGTTTCTTCGATCGCTTGATGGCGGACCCGTCCGCCATCCGGGAgg < 2:965754/87‑1 (MQ=255) ttCGAAATTTCCCTGGTAGGCCGGCTCGACACGCAATGAGTGGTTTCTTCGATCGCTTGATGGCGGACCCGTCCGCCATCCGGGAgg > 1:965754/1‑87 (MQ=255) ttCGAAATTTCCCTGGTAGGCCGGCTCGACACGCAATGAGTGGTTTCTTCGATCGCTTGATGGCGGACCCGTCCGCCATCCGGGAGGc > 2:426289/1‑88 (MQ=255) ttCGAAATTTCCCTGGTAGGCCGGCTCGACACGCAATGAGTGGTTTCTTCGATCGCTTGATGGCGGACCCGTCCGCCATCCGGGAGGc < 1:426289/88‑1 (MQ=255) ttCGAAATTTCCCTGGTAGGCCGGCTCGACACGCAATGAGTGGTTTCTTCGATCGCTTGATGGCGGACCCGTCCGCCATCCGGGAGGCGTCTCGCCAGGAAAGCGCTTCGCACAAGTTCGCGGCCATCAAGTGCCACCTCGAAACGTTGc > 2:708032/1‑150 (MQ=255) ttCCCTGGTAGGCCGGCTCGACACGCAATGAGTGGTTTCTTCGATCGCTTGATGGCGGACCCGTCCGCCATCCGGGAGGCGTCTCGCCAGGAAAGCGCTTCGCACAAGTTCGCGGCCATCAAGTGCCACCTCGAAACGTTGCTg < 1:551820/144‑1 (MQ=255) ttCCCTGGTAGGCCGGCTCGACACGCAATGAGTGGTTTCTTCGATCGCTTGATGGCGGACCCGTCCGCCATCCGGGAGGCGTCTCGCCAGGAAAGCGCTTCGCACAAGTTCGCGGCCATCAAGTGCCACCTCGAAACGTTGCTg > 2:551820/1‑144 (MQ=255) cccTGGTAGGCCGGCTCGACACGCAATGAGTGGTTTCTTCGATCGCTTGATGGCGGACCCGTCCGCCATCCGGGAGGCGTCTCGCCAGGAAAGCGCTTCGCACAAGtt > 2:748408/1‑108 (MQ=255) cccTGGTAGGCCGGCTCGACACGCAATGAGTGGTTTCTTCGATCGCTTGATGGCGGACCCGTCCGCCATCCGGGAGGCGTCTCGCCAGGAAAGCGCTTCGCACAAGtt < 1:748408/108‑1 (MQ=255) ccTGGTAGGCCGGCTCGACACGCAATGAGTGGTTTCTTCGATCGCTTGATGGCGGACCCGTCCGCCATCCGGGAGGCGtctc > 1:581380/1‑82 (MQ=255) ccTGGTAGGCCGGCTCGACACGCAATGAGTGGTTTCTTCGATCGCTTGATGGCGGACCCGTCCGCCATCCGGGAGGCGtctc < 2:581380/82‑1 (MQ=255) gTAGGCCGGCTCGACACGCAATGAGTGGTTTCTTCGATCGCTTGATGGCGGACCCGTCCGCCATCCGGGAGGCGTCTCGCCa > 1:622940/1‑82 (MQ=255) gTAGGCCGGCTCGACACGCAATGAGTGGTTTCTTCGATCGCTTGATGGCGGACCCGTCCGCCATCCGGGAGGCGTCTCGCCa < 2:622940/82‑1 (MQ=255) gTAGGCCGGCTCGACACGCAATGAGTGGTTTCTTCGATCGCTTGATGGCGGACCCGTCCGCCATCCGGGAGGCGTCTCGCCAGGAAAGCGCTTCGCACAAGTTCGCGGCCATCAAGTGCCACCTCGAAACGTTGCTGAACGCTCGCCAAg > 2:737498/1‑150 (MQ=255) acacGCAATGAGTGGTTTCTTCGATCGCTTGATGGCGGACCCGTCCGCCATCCGGGAGGCGTCTCGCCAGGAAAGCGCTTCGCACAAGTTCGCGGCCATCAAGTGCCACCTCGAAACGTTGCTGAACGCTCGCCAAGGTTGCTCCCagag < 2:1178509/150‑1 (MQ=255) acGCAATGAGTGGTTTGTACGAACCCTTGATGGCGGACCCGTCCGCCCTCCAAGAGGCGCATCGCCAGGAAAGCGCTTGTCACAAGTTAGCTGCGATCAAGTGCGACCACGAAAAGATGCTTAACGCTCGCGAAGGTTGCTGCCAGagcc > 2:588168/1‑149 (MQ=255) ggTTTCTTCGATCGCTTGATGGCGGACCCGTCCGCCATCCGGGAGGCGTCTCGCCAGGAAAGCGCTTCGCACAAGTTCGCGGCCATCAAGTGCCACCTCGAAACGTTGCTGAACGCTCGCCAAGGTTGCTCCCAGAGCAGCCCCGAGCTg < 2:623741/150‑1 (MQ=255) tttCTTCGATCGCTTGATGGCGGACCCGTCCGCCATCCGGGAGGCGTCTCGCCAGGAAAGCGCTTCGCACAAGTTCGCGGCCATCAAGTGCCACCTCGAAACGTTGCTGAACGCTCGCCAAGGTTGCTCCCAGAGCAGCCCCGa < 2:372489/144‑1 (MQ=255) tttCTTCGATCGCTTGATGGCGGACCCGTCCGCCATCCGGGAGGCGTCTCGCCAGGAAAGCGCTTCGCACAAGTTCGCGGCCATCAAGTGCCACCTCGAAACGTTGCTGAACGCTCGCCAAGGTTGCTCCCAGAGCAGCCCCGa > 1:372489/1‑144 (MQ=255) ttcGATCGCTTGATGGCGGACCCGTCCGCCATCCGGGAGGCGTCTCGCCAGGAAAGCGCTTCGCACAAGTTCGCGGCCATCAAGTGCCACCTCGAAACGTTGCTGAACGCTCGCCAAGGTTGCTCCCAGAGCAGCCCCGAGCTGGGCCTg > 2:1015435/1‑150 (MQ=255) aTCGCTTGATGGCGGACCCGTCCGCCATCCGGGAGGCGTCTCGCCAGGAAAGCGCTTCGCACAAGTTCGCGGCCATCAAGTGCCACCTCGAAACGTTGCTGAACGCTCGCCAAGGTTGCTCCCAGAGCAGCCCCGAGCTGGGCCTGcgcg > 1:372310/1‑150 (MQ=255) cggATGGCGGACGGGTCCGCCATCCGGGAGGCGTCTCGCCAGGAAAGCGCTTCGCACAAGTTCGCGGCCATCAAGTGCCACCTCGAAACGTTGCTGAACGCTCGCCAAGGTTGCTCCCAGAGCAGCCCCGAGCTGGGCCTGCGCGACTTc > 1:851488/3‑150 (MQ=255) cGGACGGGTCCGCCATCCGGGAGGCGTCTCGCCAGGAAAGCGCTTCGCACAAGTTCGCGGCCATCAAGTGCCACCTCGAAACGTTGCTGAACGCTCGCCAAGGTTGCTCCCAGAGCAGCCCCGAGCTGGGCCTGCGCGACTTCAACGGGc < 2:851488/150‑1 (MQ=255) cGGACCCGTCCGCCATCCGGGAGGCGTCTCGCCAGGAAAGCGCTTCGCACAAGTTCGCGGCCATCAAGTGCCACCTCGAAACGTTGCTGAACGCTCGCCAAGGTTGCTCCCAGAGCAGCCCCGAGCTGGGCCTGCGCGACTTCAACGGGc < 2:13817/150‑1 (MQ=255) cGGACCCGTCCGCCATCCGGGAGGCGTCTCGCCAGGAAAGCGCTTCGCACAAGTTCGCGGCCATCAAGTGCCACCTCGAAACGTTGCTGAACGCTCGCCAAGGTTGCTCCCAGAGCAGCCCCGAGCTGGGCCTGCGCGACTTCAACGGGc < 2:911432/150‑1 (MQ=255) ggACCCGTCCGCCATCCGGGAGGCGTCTCGCCAGGAAAGCGCTTCGCACAAGTTCGCGGCCATCAAGTGCCACCTCGAAACGTTGCTGAACGCTCGCCAAGGTTGCTCCCAGAGCAGCCCCGAGCTGGGCCTGCGCGACTTCAACGGGCa < 1:708032/150‑1 (MQ=255) aCCCGTCCGCCATCCGGGAGGCGTCTCGCCAGGAAAGCGCTTCGCACAAGTTCGCGGCCATCAAGTGCCACCTCGAAACGTTGCTGAACGCTCGCCAAGGTTGCTCCCAGAGCAGCCCCGAGCTGGGCCTGCGCGACTTCAACGGGCAt < 2:489887/149‑1 (MQ=255) aCCCGTCCGCCATCCGGGAGGCGTCTCGCCAGGAAAGCGCTTCGCACAAGTTCGCGGCCATCAAGTGCCACCTCGAAACGTTGCTGAACGCTCGCCAAGGTTGCTCCCAGAGCAGCCCCGAGCTGGGCCTGCGCGACTTCAACGGGCAt > 1:489887/1‑149 (MQ=255) | ACGTGGCGGGTGATCCGGGCTGGTACCAGGTCTCGCTCGCCGTGCGCCCGCACTTCAAGTACATGGGCGCGAACTTCGAAATTTCCCTGGTAGGCCGGCTCGACACGCAATGAGTGGTTTCTTCGATCGCTTGATGGCGGACCCGTCCGCCATCCGGGAGGCGTCTCGCCAGGAAAGCGCTTCGCACAAGTTCGCGGCCATCAAGTGCCACCTCGAAACGTTGCTGAACGCTCGCCAAGGTTGCTCCCAGAGCAGCCCCGAGCTGGGCCTGCGCGACTTCAACGGGCAT > NC_002947/4604108‑4604396 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |