| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 4,706,233 | G→T | 7.3% | P102H (CCC→CAC) | PP_4162 ← | membrane protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 4,706,233 | 0 | G | T | 7.3% | 169.5 / 2.9 | 55 | P102H (CCC→CAC) | PP_4162 | membrane protein |
| Reads supporting (aligned to +/- strand): ref base G (31/20); new base T (2/2); total (33/22) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.94e-01 | |||||||||||
CTGCGCGCCAGCAACCAGTTGCCCACCTGCCCAAAGTAAGGCTTGATCGAGGTAAGCAAGGCTGCGCCTTTCTGGTCCAGCGATTGCCAACCGTTGACCAGACGCTCGCCAACGA‑AAGGAATGCCACCCAGCCAGGCCGGTGCATCGGGCAGGCCGTCGACCTGCACGTCACGCACGAACGCCGTAGCGTCGCGAATGTGATCGGCCAGGTTGAACCCCAGCCACACCAGCGGCAGTGCCACGATCAGGATCCAGGCCATGGTCAGCAGGCCGGCGGCCAGCGTCTCGCGACCGC > NC_002947/4706086‑4706380 | ctgcgcgcCAGCAACCAGTTGCCCACCTGCCCAAAGTAAGGCTTGATCGAGGTAAGCAAGGCTGCGCCTTTCTGGTCCAGCGATTGCCAACCGTTGACCAGACGCTCGCCAACGA‑AAGGAATGCCACCCAGCCAGGCCGGTGCATCGGGc < 2:261324/150‑1 (MQ=255) tgcgcgcCAGCAACCAGTTGCCCACCTGCCCAAAGTAAGGCTTGATCGAGGTAAGCAAGGCTGCGCCTTTCTGGTCCAGCGATTGCCAACCGTTAAGGGGTCGAT‑ACCCACGAGTGGGACGGCCACCCAGCCAGGCCGGTGCATCGGGCa < 1:151857/150‑1 (MQ=255) cAGCAACCAGTTTCCGACCGGCCCAAAGTAAGGCTTGATCACGATGTGCAAGGCTGCGCGTTTCTTGTCCAGCGATTGCCAACCGTTGACCAGACGCTCGCCAACGA‑AAGGAATGCCACCCAGCCAGGCCGGTGCATCGGGCAGGCCGTc > 2:347395/1‑150 (MQ=255) aaCCAGTTGCCCACCTGCCCAAAGTAAGGCTTGATCGAGGTAAGCAAGGCTGCGCCTTTCTGGTCCAGCGATTGCCAACCGTTGACCAGACGCTCGCCAACGA‑AAGGAATGCCACCCAGCCAGGCCGGTGCATCGTGCAGGTCGACggcc > 1:821481/1‑147 (MQ=255) aCCAGTTGCCCACCTGCCCAAAGTAAGGCTTGATCGAGGTAAGCAAGGCTGCGCCTTTCTGGTCCAGCGATTGCCAACCGTTGACCAGACGCTCGCCAACGA‑AAGGAATGCCACCCAGCCAGGCCGGTGCATCGGGCAGGCCGTCGACCt > 1:541485/1‑150 (MQ=255) ttGCCCACCTGCCCAAAGTAAGGCTTGATCGAGGTAAGCAAGGCTGCGCCTTTCTGGTCCAGCGATTGCCAACCGTTGACCAGACGCTCGCCAACGA‑AAGGAATGCCACCCAGCCAGGCCGGTGCATCGTGCAGGTCGACGGCCTGCCCg < 2:821481/150‑1 (MQ=255) ttGCCCACCTGCCCAAAGTAAGGCTTGATCGAGGTAAGCAAGGCTGCGCCTTTCTGGTCCAGCGATTGCCAACCGTTGACCAGACGCTCGCCAACGA‑AAGGAATGCCACCCAGCCAGGCCGGTGCATCGGGCAGGCCGTCGACCTGCACg > 1:65409/1‑150 (MQ=255) cccAAAGTAAGGCTTGATCGAGGTAAGCAAGGCTGCGCCTTTCTGGTCCAGCGATTGCCAACCGTTGACCAGACGCTCGCCAACGA‑AAGGAATGCCACCCAGCCAGGCCGGTGCATCGTGCAGGTCGACGGCCTGcccga > 1:810432/1‑136 (MQ=255) cccAAAGTAAGGCTTGATCGAGGTAAGCAAGGCTGCGCCTTTCTGGTCCAGCGATTGCCAACCGTTGACCAGACGCTCGCCAACGA‑AAGGAATGCCACCCAGCCAGGCCGGTGCATCGTGCAGGTCGACGGCCTGCCCga < 2:810432/140‑2 (MQ=255) cccAAAGTAAGGCTTGATCGAGGTAAGCAAGGCTGCGCCTTTCTGGTCCAGCGATTGCCAACCGTTGACCAGACGCTCGCCAACGA‑AAGGAATGCCACCCAGCCAGGCCGGTGCATCGGGCAGGCCGTCGACCTGCACGTCACGCACGaa > 1:5599/1‑150 (MQ=255) ccAAAGTAAGGCTTGATCGAGGTAAGCAAGGCTGCGCCTTTCTGGTCCAGCGATTGCCAACCGTTGACCAGACGCTCGCCAACGA‑AAGGAATGCCACCCAGCCAGGCCGGTGCATCGGGCAGGCCGTCGACCTGCACGTCACGCACGAAc > 2:1052015/1‑150 (MQ=255) ccAAAGTAAGGCTTGATCGAGGTAAGCAAGGCTGCGCCTTTCTGGTCCAGCGATTGCCAACCGTTGACCAGACGCTCGCCAACGA‑AAGGAATGCCACCCAGCCAGGCCGGTGCATCGGGCAGGCCGTCGACCTGCACGTCACGCACGAAc > 1:743391/1‑150 (MQ=255) tAAGGCTTGATCGAGGTAAGCAACGCTGCGCCTTTCTGGTCCAGCCATTGCCAACCGTTGACCAGACCCTCGCCAATGA‑AAGGAATGCCACCCAGCCAGGCCGGTGCATCGGGTTTTCCGTCGACCTGCACGTCACGCACGAACGCCGTa < 1:401032/150‑1 (MQ=255) aGGCTTGATCGAGGTAAGAAAGGCTGCGCCTTTCTGGTCCAGCGATTGCCAACCGTTGACCAGACGCTCGCCAACGA‑AAGGGACCACACGGAGCGTGGCCGGTGCATCGGGCAGGCCGTCGACCTGCACGTCACGCACGAACGCCGTAGc > 1:445051/1‑150 (MQ=255) ggCTTGATCGAGGTAAGCAAGGCTGCGCCTTTCTGGTCCAGCGATTGCCAACCGTTGACCAGACGCTCGCCAACGA‑ACGGCCCTGCTGCCAGCCAGGCCGGTGCATCGGGCAGGCCGTCGACCTCCACGTCACGCACGAACGCCGTAGCg > 1:677495/1‑150 (MQ=255) cTTGATCGAGGTAAGCAAGGCTGCGCCTTTCTGGTCCAGCGATTGCCAACCGTTGACCAGACGCTCGCCAACGA‑AAGGAATGCCACCCAGCCAGGCCGGTGCATCGGGCAGGCCGTCGACCTGCACGTCACGCACGAACGCCGTAGc < 1:688807/147‑1 (MQ=255) cTTGATCGAGGTAAGCAAGGCTGCGCCTTTCTGGTCCAGCGATTGCCAACCGTTGACCAGACGCTCGCCAACGA‑AAGGAATGCCACCCAGCCAGGCCGGTGCATCGGGCAGGCCGTCGACCTGCACGTCACGCACGAACGCCGTAGc > 2:688807/1‑147 (MQ=255) tCGAGGTAAGCAAGGCTGCGCCTTTCTGGTCCAGCGATTGCCAACCGTTGACCAGACGCTCGCCAACGA‑AAGGAATGCCACCCAGCCAGGCCGGTGCATCGGGCAGGCCGTCGACCTGCACGTCACGCACGAACGCCGTAGCGTCGCGaa < 2:5599/150‑1 (MQ=255) cGAGGTAAGCAAGGCTGCGCCTTTCTGGTGGGGCGGGGGGCGACCGTTGACCAGACGCTCGCCAACGA‑AAGGAATGCCACCCAGCCAGGCCTGTGCATCGGGCAGGCCGTCGACCTGCACGTCACGCACGAACGCCGTAGCGTCGCGAAt < 2:399733/150‑1 (MQ=255) gTAAGCAAGGCTGCGCCTTTCTGGTCCAGCGATTGCCAACCGTTGACCAGACGCTCGCCAACGA‑AAGGAATGCCACCCAGCCAGGCCGGTGCATCGGGCAGGCCGTCGACCTGc > 1:541994/1‑114 (MQ=255) gTAAGCAAGGCTGCGCCTTTCTGGTCCAGCGATTGCCAACCGTTGACCAGACGCTCGCCAACGA‑AAGGAATGCCACCCAGCCAGGCCGGTGCATCGGGCAGGCCGTCGACCTGc < 2:541994/114‑1 (MQ=255) aGCAAGGCTGCGCCTTTCTGGTCCAGCGATTGCCAACCGTTGACCAGACGCTCGCCAACGA‑AAGGAATGCCACCCAGCCAGGCCGGTGCATCggg < 2:406825/95‑1 (MQ=255) aGCAAGGCTGCGCCTTTCTGGTCCAGCGATTGCCAACCGTTGACCAGACGCTCGCCAACGA‑AAGGAATGCCACCCAGCCAGGCCGGTGCATCggg > 1:406825/1‑95 (MQ=255) aGCAAGGCTGCGCCTTTCTGGTCCAGCGATTGCCAACCGTTGACCAGACGCTCGCCAACGA‑AAGGAATGCCACCCAGCCAGGCCGGTGCATCGGGCAGGCCGTCGACCTGCACGTAACGCACGAACGCCGTAGCGTCGCGAATGTGATCg > 1:887484/1‑150 (MQ=255) aaGGCTGCGCCTTTCTGGTCCAGCGATTGCCAACCGTTGACCAGACGCTCGCCAACGA‑AAGGAATGCCACCCAGCCAGGCCGGTGCATCGGGCAGGCCGTCGAc < 2:98548/104‑1 (MQ=255) aaGGCTGCGCCTTTCTGGTCCAGCGATTGCCAACCGTTGACCAGACGCTCGCCAACGA‑AAGGAATGCCACCCAGCCAGGCCGGTGCATCGGGCAGGCCGTCGAc > 1:98548/1‑104 (MQ=255) gCTGCGCCTTTCTGGTCCAGCGATTGCCAACCGTTGACCAGACGCTCGCCAACGA‑AAGGAATGCCACCCAGCCAGGCCGGTGCATCGGGCAGGCCGTCGACCTGCACGTCACGCACGAACGCCGTAGCGTCGCGAATGTGATCGGCCAgg > 1:613838/1‑150 (MQ=255) ccTTTCTGGTCCAGCGATTGCCAACCGTTGACCAGACGCTCGCCAACGA‑AAGGAATGCCACCCAGCCAGGCCGGTGCATCGGGCAGGCCGTCGACCTGCACGTCACGCACGAACGCCGTAGCGTCGCGAATGTGATCGGCCAGGTTGAAc < 2:743391/150‑1 (MQ=255) cAGCGATTGCCAACCGTTGACCAGACGCTCGCCAACGA‑AAGGAATGCCACCCAGCCAGGCCGGTGCATCGGGCAGGCCGTCGACCTGCACGTCACGCACGAACGCCGTAGCGTCGCGAATGTGATCGGCCAGGTTGAACCCCATCCacac > 1:253079/1‑150 (MQ=255) gCGATTGCCAACCGTTGACCAGACGCTCGCCAACGA‑AAGGAATGCCACCCAGCCAGGCCGGTGCATCGGGCAGGCCGTCGACCTGCACGTCACGCACGAACGCCGTAGCGTCGCGAATGTGATCGGCCAGGTTGAACCCCAGCCACACCa > 1:515851/1‑150 (MQ=255) cGATTGCCAACCGTTGACCAGACGCTCGCCAACGA‑AAGGAATGCCACCCAGCCAGGCCGGTGCATCGGGCAGGCCGTCGACCTGCACGTCACGCACGAACGCCGTAGCGTCGCGAATGTGATCGGCCAGGTTGAACCCCAGCCACACCAg < 2:65409/150‑1 (MQ=255) gCCAACCGTTGACCAGACGCTCGCCAACGA‑AAGGAATGCCACCCAGCCAGGCCGGTGCATCGGGCAGGCCGTCGACCTGCACGTCACGCACGAACGCCGTAGCGTCGCGAAt > 1:983158/1‑112 (MQ=255) gCCAACCGTTGACCAGACGCTCGCCAACGA‑AAGGAATGCCACCCAGCCAGGCCGGTGCATCGGGCAGGCCGTCGACCTGCACGTCACGCACGAACGCCGTAGCGTCGCGAAt < 2:983158/112‑1 (MQ=255) aaCCGTTGACCAGACGCTCGCCAACGA‑AAGGAATGCCACCCAGCCAGGCCGGTGCATCGGGCAGGCCGTCGACCTGCACGTCACGCACGAACGCCGCAGCGTcgc > 2:320874/1‑105 (MQ=255) aaCCGTTGACCAGACGCTCGCCAACGA‑AAGGAATGCCACCCAGCCAGGCCGGTGCATCGGGCAGGCCGTCGACCTGCACGTCACGCACGAACGCCGCAGCGTcgc < 1:320874/105‑1 (MQ=255) aCCGTTGACCAGACGGTCGCCAACGA‑AAGTAATGCCACCCATCCAGGCCGGTGCATCGGGCAGGCCGTCGACCTGCACGTCACGCACGAACGCCGTAGCGTCGCGAATGTGATCGGCCAGGTTGAACCCCAGCCACACCAGCGGCAGTGc < 2:268565/150‑1 (MQ=255) cGTTGACCAGACGCTCGCCAACGA‑AAGGAATGCCACCCAGCCAGGCCGGTGCATCGGGCAGGCCGTCGACCTGCACGTCACGCACGAACGCCGTAGCGTCGCGAATGTGATCGGCCAGGTTGAACACCAGCCACACCAGCGGCAGTGCCa > 2:234963/1‑150 (MQ=255) aCCAGACGCTCGCCAACGA‑AAGGAATGCCACCCAGCCAGGCCGGTGCCTCGGGCAGGCCGTCGACCTGCACGTCACGCACGAACGCCGTAGCGTCGCGAATGTGATCGGCCAGGTTGAACCCCAGCCACACCAGCGGCAGTGCCACGATc > 2:1093230/1‑150 (MQ=255) cAGACGCTCGCCAACGA‑AAGGAATGCCACCCAGCCAGGCCGGTGCATCGGGCAGGCCGTCGACCTGCACGTCACGCACGAACGCCGTAGCGTCGCGAATGTGATCGGCCAGGTTGAACCCCAGCCACACCAGCGGCAGTGCCACGATCAg > 1:883210/1‑150 (MQ=255) tCGCCAACGA‑AAGGAATGCCACCCAGCCAGGCCGGTGCATCGGGCAGGCCGTCGACCTGCACGTCACGCACGAACGCCGTAGCGTCGCGAATGTGATCGGCCAGGTTGAACCCCCGACCCACAAGCGGCAGTCCCACTTTTCGGATCCAg < 2:677495/150‑1 (MQ=255) cAACGA‑AAGGAATGCCACCCAGCCAGGCCGGTGCATCGGGCAGGCCGTCGACCTGCACGTCACGCACGAACGCCGTAGCGTCGCGAATGTGATCGGCCAGGTTGAACCCCAGCCACACCAGCGGCAGTGCCACGATCAGGATCCAGGCCa < 1:799105/150‑1 (MQ=255) aCGA‑AAGGAATGCCACCCAGCCAGGCCGGTGCATCGGGCAGGCCGTCGACCTGCACGTCACGCACGAACGCCGTAGCGTCGCGAATGTGATCGGCCAGGTTGAACCCCAGCCACACCAGCGGCAGTGCCACGATCAGGATCCAGGCCATg < 2:445051/150‑1 (MQ=255) gA‑AAGGAATGCCACCCAGCCAGGCCGGTGCATCGGGCAGGCCGTCGACCTGCACGTCACGCACGAACGCCGTAGCGTCGCGAATGTGATCGGCCAGGtt < 2:240281/99‑1 (MQ=255) gA‑AAGGAATGCCACCCAGCCAGGCCGGTGCATCGGGCAGGCCGTCGACCTGCACGTCACGCACGAACGCCGTAGCGTCGCGAATGTGATCGGCCAGGtt > 1:240281/1‑99 (MQ=255) gA‑AAGGAATGCCACCCAGCCAGGCCGGTGCATCGGGCAGGCCGTCGACCTGCACGTCACGCACGAACGCCGTAGCGTCGCGAATGTGATCGGCCAGGTTGAACCCCAGCCACACCAGCGGCAGTGCCACGATCAGGATCCAGGCCATGGt > 1:1192254/1‑150 (MQ=255) a‑aaGGAATGCCACCCAGCCAGGCCGGTGCATCGGGCAGGCCGTCGACCTGCACGTCACGCACGAACGCCGTAGCGTCGCGAATGTGATCGGCCAGGTTGAACCCCAGCCACACCAGCGGCAGTGCCACGATCAGGATCCAGGCCATGGTc > 1:989019/1‑150 (MQ=255) a‑aaGGAATGCCACCCAGCCAGGCCGGTGCATCGGGCAGGCCGTCGACCTGCACGTCACGCACGAACGCCGTAGCGTCGACAAAGTGATCGGCCAGGTTGAACCCCAGCCACACCAGCGGCAGTGCCACGATCAGGATCCAGGCCATGGTc > 1:811042/1‑150 (MQ=255) tGCCACCCAGCCAGGCCGGTGCATCGGGCAGGCCGTCGACCTGCACGTCACGCACGAACGCCGTAGCGTCGCGAATGTGATCGGCCAGGTTGAACCCCAGCCACACCAGCGGCAGTGCCACGATCAGGATCCAGGCCATGGTCAGCAGGc > 1:349435/1‑150 (MQ=255) cACCCAGCCAGGCCGGTGCATCGGGCAGGCCGTCGACCTGCACGTCACGCACGAACGCCGTAGCGTCGCGAATGTGAt > 1:692995/1‑78 (MQ=255) cACCCAGCCAGGCCGGTGCATCGGGCAGGCCGTCGACCTGCACGTCACGCACGAACGCCGTAGCGTCGCGAATGTGAt < 2:692995/78‑1 (MQ=255) cGGTGCATCGGGCAGGCCGTCGACCTGCACGTCACGCACGAACGCCGTAGCGTCGCGAATGTGATCGGCCAGGTTGAAcccc < 2:544144/82‑1 (MQ=255) cGGTGCATCGGGCAGGCCGTCGACCTGCACGTCACGCACGAACGCCGTAGCGTCGCGAATGTGATCGGCCAGGTTGAAcccc > 1:544144/1‑82 (MQ=255) cATCGGGCAGGCCGTCGACCTGCACGTCACGCACGAACGCCGTAGCGTCGCGAATGTGATCGGCCAGGTTGAACCCCa < 2:124240/78‑1 (MQ=255) cATCGGGCAGGCCGTCGACCTGCACGTCACGCACGAACGCCGTAGCGTCGCGAATGTGATCGGCCAGGTTGAACCCCa > 1:124240/1‑78 (MQ=255) cATCGGGCAGGCCGTCGACCTGCACGTCACGCACGAACGCCGTAGCGTCGCGAATGTGATCGGCCAGGTTGAACCCCAGCCACACCAGCGGCAGTGCCACGATCAGGATCCAGGCCATGGTCAGCAGGCCGGCGGCCAGCGTCTCGCGAc > 2:87597/1‑150 (MQ=255) aTCGGGCAGGCCGTCGACCTGCACGTCACGCACGAACGCCGTAGCGTCGCGAATGTGATCGGCCAGGTTGAACCCCAGCCACACCAGCGGCAGTGCCACGATCAGGATCCAGGCCATGGTCAGCAGGCCGGCGGCCAGCGTCTCGCGAcc > 2:319657/1‑150 (MQ=255) cGGGCAGGCCGTCGACCTGCACGTCACGCACGAACGCCGTAGCGTCGCGAATGTGATCGGCCAGGTTGAACCCCAGCCACACCAGCGGCAGTGCCACGATCAGGATCCAGGCCATGGTCAGCAGGCCGGCGGCCAGCGTCTCGCGACCGc > 2:1113684/1‑150 (MQ=255) | CTGCGCGCCAGCAACCAGTTGCCCACCTGCCCAAAGTAAGGCTTGATCGAGGTAAGCAAGGCTGCGCCTTTCTGGTCCAGCGATTGCCAACCGTTGACCAGACGCTCGCCAACGA‑AAGGAATGCCACCCAGCCAGGCCGGTGCATCGGGCAGGCCGTCGACCTGCACGTCACGCACGAACGCCGTAGCGTCGCGAATGTGATCGGCCAGGTTGAACCCCAGCCACACCAGCGGCAGTGCCACGATCAGGATCCAGGCCATGGTCAGCAGGCCGGCGGCCAGCGTCTCGCGACCGC > NC_002947/4706086‑4706380 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |