Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,717,858 G→A 8.3% R126H (CGC→CAC)  fabB → 3‑oxoacyl‑ACP synthase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,717,8580GA 8.3% 130.4 / 2.8 48R126H (CGC→CAC) fabB3‑oxoacyl‑ACP synthase
Reads supporting (aligned to +/- strand):  ref base G (20/24);  new base A (2/2);  total (22/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.31e-01

CTGGCCATGCAGGACGCGATCAAGGACGCTGGCCTGACCGAAGAGCAGGTTTCCAACCCGCGTACCGGCCTGGTGGCGGGTTCCGGCGGCGCCTCGACCCTGAACCAGATGGAAGCGCTGGACACCCTGCGTGAGAAAGGCGTCAAGCGCGTCGGCCCATACCGCGTTACCCGCACCATGGGCAGCACCGTGTCGGCATGCCTGGCCACCCCGTTCAAGATCAAGGGCATCAACTACTCGATCTCGTCGGCCTGCGCCACCTCGGCTCACTGCATCGGTACCGCACTGGAGCAGATCC  >  NC_002947/4717710‑4718007
                                                                                                                                                    |                                                                                                                                                     
cTGGCCATGCAGGACGCGATCAAGGACGCTGGCCTGACCGAAGAGCAGGTTTCCAACCCGCGTACCGGCCTGGTGGCGGGTTCCGGCGGCGCCTCGACCCTGAACCAGATGGAAGCGCTGGACACCCTGCGTGAGAAAGGCGTCAAgcgc                                                                                                                                                      >  1:1061704/1‑150 (MQ=255)
cTGGCCATGCAGGACGCGATCAAGGACGCTGGCCTGACCGAAGAGCAGGTTTCCAACCCGCGTACCGGCCTGGTGGCGGGTTCCGGCGGCGCCTCGACCCTGAACCAGATGGAAGCGCTGGACACCCTGCGTGAGAAAGGCGTCAAgcgc                                                                                                                                                      >  2:12633/1‑150 (MQ=255)
  ggCCATGCAGGACGCGATCAAGGACGCTGGCCTGACCGAAGAGCAGGTTTCCAACCCGCGTACCGGCCTGGTGGCGGGTTCCGGCGGCGCCTCGACCCTGAACCAGATGGAAGCGCTGGACACCCTGCGTGAGAAAGGCGTCAAGCGCGt                                                                                                                                                    <  2:960713/150‑1 (MQ=255)
   gCCATGCAGGACGCGATCAAGGACGCTGGCCTGACCGAAGAGCAGGTTTCCAACCCGCGTACCGGCCTGGTGGCGGGTTCCGGCGGCGCCTCGACCCTGAACCAGATGGAAGCGCTGGACACCCTGCGTGAGAAAGGCGTCAAGCGCGTc                                                                                                                                                   >  2:1086198/1‑150 (MQ=255)
      aTGCAGGACGCGATCAAGGACGCTGGCCTGACCGAAGAGCAGGTTTCCAACCCGCGTACCGGCCTGGTGGCGGGTTCCGGCGGCGCCTCGACCCTGAACCAGATGGAAGCGCTGGACACCCTGCGTGAGAAAGGCGTCAAGCGCGTCGGc                                                                                                                                                <  1:3286/150‑1 (MQ=255)
          aGGACGCGATCAAGGACGCTGGCCTGACCGAAGAGCAGGTTTCCAACCCGCGTACCGGCCTGGTGGCGGGTTCCGGCGGCGCCTCGACCCTGAACCAGATGGAAGCGCTGGACACCCTGCGTGAGAAAGGCGTCAAGCGCGTCGGCCCAt                                                                                                                                            >  2:296821/1‑150 (MQ=255)
               gcgATCAAGGACGCTGGCCTGACCGAAGAGCAGGTTTCCAACCCGCGTACCGGCCTGGTGGCGGGTTCCGGCGGCGCCTCGACCCTGAACCAGATGGAAGCGCTGGACACCCTGCGTGAGAAAGGCGTCAAGCGCGTCGGCCCATACcgc                                                                                                                                       >  2:686800/1‑150 (MQ=255)
               gcgATCAAGGACGCTGGCCTGACCGAAGAGCAGGTTTCCAACCCGCGTACCGGCCTGGTGGCGGGTTCCGGCGGCGCCTCGACCCTGAACCAGATGGAAGCGCTGGACACCCTGCGTGAGAAAGGCGTCAAGCGCGTCGGCCCATACcgc                                                                                                                                       <  1:296821/150‑1 (MQ=255)
                 gATCAAGGACGCTGGCCTGACCGAAGAGCAGGTTTCCAACCCGCGTACCGGCCTGGTGGCGGGTTCCGGCGGCGCCTCGACCCTGAACCAGATGGAAGCGCTGGACACCCTGCGTGAGAAAGGCGTCAAGCGCGTCGGc                                                                                                                                                <  1:545713/139‑1 (MQ=255)
                 gATCAAGGACGCTGGCCTGACCGAAGAGCAGGTTTCCAACCCGCGTACCGGCCTGGTGGCGGGTTCCGGCGGCGCCTCGACCCTGAACCAGATGGAAGCGCTGGACACCCTGCGTGAGAAAGGCGTCAAGCGCGTCGGc                                                                                                                                                >  2:545713/1‑139 (MQ=255)
                  aTCAAGGACGCTGGCCTGACCGAAGAGCAGGTTTCCAACCCGCGTACCGGCCTGGTGGCGGGTTCCGGCGGCGCCTCGACCCTGAACCAGATGGAAGCGCTGGACACCCTGCGTGAGAAAGGCGTCAAGCGCGTCGGCCCATACCGCGtt                                                                                                                                    <  1:167408/150‑1 (MQ=255)
                     aaGGACGCTGGCCTGACCGAAGAGCAGGTTTCCAACCCGCGTACCGGCCTGGTGGCGGGTTCCGGCGGCGCCTCTACCCTGAACCAGATTGAAGCGCTGGACACCCTGCGTGAGAAAGGCGTCAAGCGCGTCGGCCCATACCGCGTTAcc                                                                                                                                 >  1:81530/1‑150 (MQ=255)
                       ggACGCTGGCCTGACCGAAGAGCAGGTTTCCAACCCGCGTACCGGCCTGGTGGCGGGTTCCGGCGGCGCCTCGACCCTGAACCAGATGGAAGCGCTGGACACCCTGCGTGAGAAAGGCGTCAAGCGCGTCGGCCCATACCGCGTTACCCg                                                                                                                               >  1:198977/1‑150 (MQ=255)
                         aCGCTGGCCTGACCGAAGAGCAGGTTTCCAACCCGCGTACCGGCCTGGTGGCGGGTTCCGGCGGCGCCTCGACCCTGAACCAGATGGAAGCGCTGGACACCCTGCGTGAGAAAGGCGTCAAGCGCGTCGGCCCATACCGCGTTACCCGCa                                                                                                                             <  1:686800/150‑1 (MQ=255)
                             tGGCCTGACCGAAGAGCAGGTTTCCAACCCGCGTACCGGCCTGGTGGCGGGTTCCGGCGGCGCCTCGACCCTGAACCAGATGGAAGCGCTGGACACCCTGCGTGAGAAAGGCGTCAAGCGCGTCGGCCCATACCGCGTTACCCGCACCAt                                                                                                                         <  2:607617/150‑1 (MQ=255)
                               gCCTGACCGAAGAGCAGGTTTCCAACCCGCGTACCGGCCTGGTGGCGGGTTCCGGCGGCGCCTCGACCCTGAACCAGATGGAAGCGCTGGACACCCTGCGTGAGAAAGGCGTCAAGCGCGTCGGCCCATACCGCGTTACCCGCACCATgg                                                                                                                       <  1:454586/150‑1 (MQ=255)
                                ccTGACCGAAGAGCAGGTTTCCAACCCGCGTACCGGCCTGGTGGCGGGTTCCGGCGGCGCCTCGACCCTGAACCAGATGGAAGCGCTGGACACCCTGCGTGAGAAAGGCGTCAAGCACGTCGGCCCATACCGCGTTACCCGCACCATggg                                                                                                                      >  1:1137789/1‑150 (MQ=255)
                                ccTGACCGAAGAGCAGGTTTCCAACCCGCGTACCGGCCTGGTGGCGGGTTCCGGCGGCGCCTCGACCCTGAACCAGATGGAAGCGCTGGACACCCTGCGTGAGAAAGGCGTCAAGCACGTCGGCCCATACCGCGTTACCCGCACCATggg                                                                                                                      >  1:1137575/1‑150 (MQ=255)
                                  tGACCGAAGAGCAGGTTTCCAACCCGCGTACCGGCCTGGTGGCGGGTTCCGGCGGCGCCTCGACCCTGAACCAGATGGAAGCGCTGGACACCCTGCGTGAGAAAGGCGTCAAGCGCGTCGGCCCATACCGCGTTACCCGCAACATggggc                                                                                                                    <  2:1079913/150‑3 (MQ=255)
                                    aCCGAAGAGCAGGTTTCCAACCCGCGTACCGGCCTGGTGGCGGGTTCCGGCGGCGCCTCGACCCTGAACCAGATGGAAGCGCTGGACACCCTGCGTGAGAAAGGCGTCAAGCGCGTCGGCCCATACCGCGTTACCCGCACCATGGgcagc                                                                                                                  <  2:888447/150‑1 (MQ=255)
                                                 tttCCAACCCGCGTACCGGCCTGGTGGCGGGTTCCGGCGGCGCCTCGACCCTGAACCAGATGGAAGCGCTGGACACCCTGCGTGAGAAAGGCGTCAAGCGCGTCGGCCCATACCGCGTTACCCGCACCATGGGCAGCACCGTGTCGGCAt                                                                                                     <  2:1061704/150‑1 (MQ=255)
                                                   tCCAACCCGCGTACCGGCCTGGTGGCGGGTTCCGGCGGCGCCTCGACCCTGAACCAGATGGAAGCGCTGGACACCCTGCGTGAGAAAGGCGTCAAGCGCGTCGGCCCATACCGCGTTACCCGCACCATGGGCAGCACCGTGTCGGCATGc                                                                                                   <  2:198977/150‑1 (MQ=255)
                                                      aaCCCGCGTACCGGCCTGGTGGCGGGTTCCGGCGGCGCCTCGACCCTGAACCAGATGTAAGCGCTGGACACCCTGCGTGAGAAAGGCGTCAAGCGCGTCGGCCCATACCGCGTTACCCGCACCATGGGCAGCACCGTGTCGGCATGCCTg                                                                                                <  1:12633/150‑1 (MQ=255)
                                                       aCCCGCGTACCGGCCTGGTGGCGGGTTCCGGCGGCGCCTCGACCCTGAACCAGATGGAAGCGCTGGACACCCTGCGTGAGAAAGGCGTCAAGCGCGTCGGCCCATACCGCGTTACCCGCACCATGGGCAGCACCGTTTCGGCATGCCTgg                                                                                               <  1:217529/150‑1 (MQ=255)
                                                                 cGGCCTGGTGGCGGGTTCCGGCGGCGCCTCGACCCTGAACCAGATGGAAGCGCTGGACACCCTGCGTGAGAAAGGCGTCAAGCGCGTCGGCCCATACCGCGTTACCCGCACCATGGGCAGCACCGTGTCGGCATGCCTGGCCACCCCGtt                                                                                     >  1:1075374/1‑150 (MQ=255)
                                                                   gCCTGGTGGCGGGTTCCGGCGGCGCCTCGACCCTGAACCAGATGGAAGCGCTGGACACCCTGCGTGAGAAAGGCGTCAAGCGCGTCGGCCCATACCGCGTTACCCGCACCATGGGCAGCACCGTGTCGGCATGCCTGGCCACCCCGTTCa                                                                                   >  2:1000074/1‑150 (MQ=255)
                                                                    ccggtacgCGGGTTCCGGCGGCGCCTCGACCCTGAACCAGATGGAAGCGCTGGACACCCTGCGTGAGAAAGGCGTCAAGCGCGTCGGCCCATACCGCGTTACCCGCACCATGGGCAGCACCGTGTCGGCATGCCTGGCCACCCCGTTCaa                                                                                  <  2:737066/143‑1 (MQ=255)
                                                                    ccTGGTGGCGGGTTCCGGCGGCGCCTCGACCCTGAACCAGATGGAAGCGCTGGACACCCTGCGTGAGAAAGGCGTCAAGCGCGTCGGCCCATACCGCGTTACCCGCACCATGGGCAGCACCGTGTCGGCATGCCTGGCCACCCCGTTCaa                                                                                  <  1:1000074/150‑1 (MQ=255)
                                                                             gggTTCCGGCGGCGCCTCGACCCTGAACCAGATGGAAGCGCTGGACACCCTGCGTGAGAAAGGCGTCAAGCGCGTCGGCCCATACCGCGTTACCCGCACCATGGGCAGCACCGTGTCGGCATGCCTGGCCAc                                                                                           <  1:1081830/132‑1 (MQ=255)
                                                                             gggTTCCGGCGGCGCCTCGACCCTGAACCAGATGGAAGCGCTGGACACCCTGCGTGAGAAAGGCGTCAAGCGCGTCGGCCCATACCGCGTTACCCGCACCATGGGCAGCACCGTGTCGGCATGCCTGGCCAc                                                                                           >  2:1081830/1‑132 (MQ=255)
                                                                                            cTCGACCCTGAACCAGATGGAAGCGCTGGACACCCTGCGTGAGAAAGGCGTCAAGCACGTCGGCCCATACCGCGTTACCCGCACCATGGGCAGCACCGTGTCGGCATGCCTGGCCACCCCGTTCAAGATCAAGGGCATCAACTACTCGAt                                                          <  2:1137575/150‑1 (MQ=255)
                                                                                            cTCGACCCTGAACCAGATGGAAGCGCTGGACACCCTGCGTGAGAAAGGCGTCAAGCACGTCGGCCCATACCGCGTTACCCGCACCATGGGCAGCACCGTGTCGGCATGCCTGGCCACCCCGTTCAAGATCAAGGGCATCAACTACTCGAt                                                          <  2:1137789/150‑1 (MQ=255)
                                                                                               gACCCTGAACCAGATGGAAGCGCTGGACACCCTGCGTGAGAAAGGCGTCAAGCGCGTCGGCCCATACCGCGTTACCCGCACCATGGGCAGCACCGTGTCGGCATGCCTGGCCACCCCGTTCAAGATCACGGGCATCAACTACTCGAtctc                                                       >  2:115730/1‑150 (MQ=255)
                                                                                               gACCCTGAACCAGATGGAAGCGCTGGACACCCTGCGTGAGAAAGCCGTCAAGCGCGTCGGCCCATACCGCGTTACCCGCACCATGGGCAGCACCGTGTCGGCATGCCTGGCCACCCCGTTCAAAATCAAGGGCATCAACTACTCGAtctc                                                       >  2:999394/1‑150 (MQ=255)
                                                                                                  ccTGAACCAGATGGAAGCGCTGGACACCCTGCGTGAGAAAGGCGTCAAGCGCGTCGGCCCATACCGCGTTACCCGCACCATGGGCAGCACCGTGTCGGCATGCCTGGCCACCCCGTTCAAGATCAAGGGCATCAACTACTCGATCtcgtc                                                    >  2:125962/1‑150 (MQ=255)
                                                                                                   cTGAACCAGATGGAAGCGCTGGACACCCTGCGTGAGAAAGGCGTCAAGCGCGTCGGCCCATACCGCGTTACCCGCACCATGGGCAGCACCGTGTCGGCATGCCTGGCCACCCCGTTCAAGATCAAg                                                                           >  2:740566/1‑126 (MQ=255)
                                                                                                   cTGAACCAGATGGAAGCGCTGGACACCCTGCGTGAGAAAGGCGTCAAGCGCGTCGGCCCATACCGCGTTACCCGCACCATGGGCAGCACCGTGTCGGCATGCCTGGCCACCCCGTTCAAGATCAAg                                                                           <  1:740566/126‑1 (MQ=255)
                                                                                                    tGAACCAGATGGAAGCGCTGGACACCCTGCGTGAGATAGGCGTCAAGCGCGTCGGCCCATACCGCGTTACCCGCACCATGGGCAGCACCGTGTCGGCATGCCTGGCCACCCCGTTCAAGATCAAGGGCATCAACTACTCGATCTCGTCgg                                                  >  1:519577/1‑150 (MQ=255)
                                                                                                       aCCAGATGGAAGCGCTGGACACCCTGCGTGAGAAAGGCGTCAAGCGCGTCGGCCCATACCGCGTTACCCGCACCATGGGCAGCACCGTGTCGGCATGCCTGGCCACCCCGTTCAAGATCAAGGGCATCAACTACTCGATCTCGTCGGCCt                                               >  1:171657/1‑150 (MQ=255)
                                                                                                           gATGGAAGCGCTGGACACCCTGCGTGAGACAGCCGTCAGGTGCGGCATCTTCCACTTGGTTACCCGCAGCAGTGGCAGCACCGTGTCGGCATGCCTGGCCACACCGTTCAAGATCAGAGGGATAAACTACTCAATCTCGTCGGCCTgcgc                                           >  1:210536/1‑150 (MQ=255)
                                                                                                                  gcgcTGGACACCCTGCGTGAGAAAGGCGTCAAGCGCGTCGGCCCATACCGCGTTACCCGCACCATGGGCAGCACCGTGTCGGCATGCCTGGCCACCCCGTTCa                                                                                   <  1:883255/103‑1 (MQ=255)
                                                                                                                  gcgcTGGACACCCTGCGTGAGAAAGGCGTCAAGCGCGTCGGCCCATACCGCGTTACCCGCACCATGGGCAGCACCGTGTCGGCATGCCTGGCCACCCCGTTCa                                                                                   >  2:883255/1‑103 (MQ=255)
                                                                                                                   cgcTGGACACCCTGCGTGAGAAAGGCGTCAAGCGCGTCGGCCCATACCGCGTTACGCGCACCATGGGCAGCACCGTGTCGGCATGCCTGGCCACCCCGTTCAAGATCAAGGGCATCAACTACTCGATCTCGTCGGCCTGCGCCACCTCgg                                   <  2:171657/150‑1 (MQ=255)
                                                                                                                    gcTGGACACCCTGCGTGAGAAAGGCGTCAAGCGCGTCGGCCCATACCGCGTTACCCGCACCATGGGCAGCACCGTGTCGGCATGCCTGGCCACCCCGTTCAAGATCAAGGGCATCAACTACTCGATCTCGTCGGCCTGCGCCACCTCGGc                                  <  1:124045/150‑1 (MQ=255)
                                                                                                                      tGGACACCCTGCGTGAGAAAGGCGTCAAGCGCGTCGGCCCATACCGCGTTACCCGCACCATGGGCAGCACCGTGTCGGCATGCCTGGCCACCCCGTTCAAGATCAAGGGCATCAACTACTCGATCTCGTCGGCCTGCGCCACCTCGGCTc                                >  1:1138400/1‑150 (MQ=255)
                                                                                                                                 cGTGAGAGAGGCGTCAAGCGCGTCGGCCCATACCGCGTTACCCGCACCATGGGCAGCACCGTGTCGGCATGCCTGGCCACCCCGTTCAAGATCAAGGGCATCAACTACTCGATCTCGTCGGCCTGCGCCACCTCGGCTCACTGCATCGGt                     <  2:519577/150‑1 (MQ=255)
                                                                                                                                   tGAGAAAGGCGTCAAGCGCGTCGGCCCATACCGCGTTACCCGCACCATGGGCAGCACCGTGTCGGCATGCCTGGCCACCCCGTTCAAGATCAAGGGCATCAACTACTCGATCTCGTCGGCCTGCGCCACCTCGGCTCACTGCATCGGTAc                   <  1:1086198/150‑1 (MQ=255)
                                                                                                                                       aaaGGCGTCAAGCGCGTCGGCCCATACCGCGTTACCCGCACCATGGGCAGCACCGTGTCGGCATGCCTGGCCACCCCGTTCAAGATCAAGGGCATCAACTACTCGATCTCGTCGGCCTGCGCCACCTCGGCTCACTGCATCGGTACCGCa               >  1:773117/1‑150 (MQ=255)
                                                                                                                                          ggCGTCAAGCGCGTCGGCCCATACCGCGTTACCCGCACCATGGGCAGCACCGTGTCGGCATGCCTGGCCACCCCGTTCAAGATCAAGGGCATCAACTACTCGATCTCGTCGGCCTGCGCCACCTCGGCTCACTGCATCGGTACCGCACTg            <  1:370868/150‑1 (MQ=255)
                                                                                                                                                aaGCGCGTCGGCCCATACCGCGTTACCCGCACCATGGGCAGCACCGTGTCGGCATGCCTGGCCACCCCGTTCAAGATCAAGGGCATCAACTACTCGATCTCGTCGGCCTGCGCCACCTCGGCTCACTGCATCGGTACCGCACTGGAGCAg      >  1:606871/1‑150 (MQ=255)
                                                                                                                                                  gcgcGTCGGCCCATACCGCGTTACCCGCACCATGGGCAGCACCGTGTCGGCATGCCTGGCCACCCCGTTCAAGATCAAGGGCATCAACTACTCGATCTCGTCGGCCTGCGCCACCTCGGCTCACTGCATCGGTACCGCACTGGAGCAGAt    >  2:382095/1‑150 (MQ=255)
                                                                                                                                                    gcgTCGGCCCATACCGCGTTACCCGCACCATGGGCAGCACCGTGTCGGCATGCCTGGCCACCCCGTTCAAGATCAAGGGCATCAACTACTCGATCTCGTCGGCCTGCGCCACCTCGGCTCACTGCATCGGTACCGCACTGGAGCAGATcc  >  2:127421/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
CTGGCCATGCAGGACGCGATCAAGGACGCTGGCCTGACCGAAGAGCAGGTTTCCAACCCGCGTACCGGCCTGGTGGCGGGTTCCGGCGGCGCCTCGACCCTGAACCAGATGGAAGCGCTGGACACCCTGCGTGAGAAAGGCGTCAAGCGCGTCGGCCCATACCGCGTTACCCGCACCATGGGCAGCACCGTGTCGGCATGCCTGGCCACCCCGTTCAAGATCAAGGGCATCAACTACTCGATCTCGTCGGCCTGCGCCACCTCGGCTCACTGCATCGGTACCGCACTGGAGCAGATCC  >  NC_002947/4717710‑4718007

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑