Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,731,726 T→C 9.6% N361D (AAT→GAT)  sucB ← 2‑oxoglutarate dehydrogenase dihydrolipoyltranssuccinylase subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,731,7260TC 9.6% 137.1 / 3.3 51N361D (AAT→GAT) sucB2‑oxoglutarate dehydrogenase dihydrolipoyltranssuccinylase subunit
Reads supporting (aligned to +/- strand):  ref base T (21/23);  new base C (2/3);  total (24/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AGGGTTAGATGTCCAGCAGCAGGCGGGACGGATCTTCCAGCAGGTTCTTGATAGTGACCAGGAAGGTTACCGCTTCCTTGCCGTCGATCAGGCGGTGATCGTACGACAGCGCCAGGTACATCATCGGGCGAATCACGACCTGGCCATTGATGGCCATCGGGCGCTGGATGATGTTGTGCATGCCGAGAATGGCGGCCTGCGGCGGGTTGAC‑GATCGGGGTCGACATCATCGAACCGAAGGTGCCACCGTTGGTGATGGTGAAGGTGCCACCGGTCATCTCTTCGATGGACAGTTTGC  >  NC_002947/4731579‑4731875
                                                                                                                                                   |                                                                                                                                                      
agGGTTAGATGTCCAGCAGCAGGCGGGACGGATCTTCCAGCAGGTTCTTGATAGTGACCAGGAAGGTTACCGCTTCCTTGCCGTCGATCAGGCGGTGATCGTACGACAGCGCCAGGTACATCATCGGGCGAATCACGACCTGGCCATTGa                                                                                                                                                      <  2:540044/150‑1 (MQ=255)
   gTTAGATGTCCAGCAGCAGGCGGGACGGATCTTCCAGCAGGTTCTTGATAGTGACCAGGAAGGTTACCGCTTCCTTGCCGTCGATCAGGCGGTGATCGTACGACAGCGCCAGGTACATCATCGGGCGAATCACGACCTGGCCATTGATgg                                                                                                                                                   <  2:157362/150‑1 (MQ=255)
    ttAGATGTCCAGCAGCAGGCGGGACGGATCTTCCAGCAGGTTCTTGATAGTGACCAGGAAGGTTACCGCTTCCTTGCCGTCGATCAGGCGGTGATCGTACGACAGCGCCAGGTACATCATCGGGCGAATCACGACCTGGCCATTGATGGc                                                                                                                                                  <  2:440435/150‑1 (MQ=255)
          gTCCAGCAGCAGGCGGGACGGATCTTCCAGCAGGTTCTTGATAGTGACCAGGAAGGTTACCGCTTCCTTGCCGTCGATCAGGCGGTGATCGTACGACAGCGCCAGGTACATCATCGGGCGAATCACGACCTGGCCATTGATGGCCATCgg                                                                                                                                            <  2:1203741/150‑1 (MQ=255)
          gTCCAGCAGCAGGCGGGACGGATCTTCCAGCAGGTTCTTGATAGTGACCAGGAAGGTTACCGCTTCCTTGCCGTCGATCAGGCGGTGATCGTACGACAGCGCCAGGTACATCATCGGGCGAATCACGACCTGGCCATTGATGGCCATCgg                                                                                                                                            >  1:1203741/1‑150 (MQ=255)
          gTCCAGCAGCAGGCGGGACGGATCTTCCAGCAGGTTCTTGATAGTGACCAGGAAGGTTACCGCTTCCTTGCCGTCGATCAGGCGGTGATCGTACGACAGCGCCAGGTACATCATCGGGCGAATCACGACCTGGCCATTGATGGCCATCgg                                                                                                                                            >  1:24397/1‑150 (MQ=255)
           tCCAGCAGCAGGCGGGACGGATCTTCCAGCAGGTTCTTGATAGTGACCAGGAAGGTTACCGCTTCCTTGCCGTCGATCAGGCGGTGATCGTACGACAGCGCCAGGTACATCATCGGGCGAATCACGACCTGGCCATTGATGGCCATCggg                                                                                                                                           >  1:809400/1‑150 (MQ=255)
           tCCAGCAGCAGGCGGGACGGATCTTCCAGCAGGTTCTTGATAGTGACCAGGAAGGTTACCGCTTCCTTGCCGTCGATCAGGCGGTGATCGTACGACAGCGCCAGGTACATCATCGGGCAAATCACGACCTGGCCATTGATGGCCATCggg                                                                                                                                           >  1:152540/1‑150 (MQ=255)
             cagcagcagGCGGGACGGATCTTCCAGCAGGTTCTTGATAGTGACCAGGAAGGTTACCGCTTCCTTGCCGTCGATCAGGCGGTGATCGTACGACAGCGCCAGGTACATCATCGGGCGAATCACGACCTGGCCATTGATGGCCATCGGgcg                                                                                                                                         >  2:787240/1‑150 (MQ=255)
             cagcagcagGCGGGACGGATCTTCCAGCAGGTTCTTGATAGTGACCAGGAAGGTTACCGCTTCCTTGCCGTCGATAAGGCGGTGATCGTACGACAGCGCCAGGTACATCATCGGGCGAATCACGACCTGGCCATTGATGGCCATCGGgcg                                                                                                                                         >  1:569341/1‑150 (MQ=255)
                       cGGGACGGATCTTCCAGCAGGTTCTTGATAGTGACCAGGAAGGTTACCGCTTCCTTGCCGTCGATCAGGCGGTGATCGTACGACAGCGCCAGGTACATCATCGGGCGAATCACGACCTGGCCATTGATGGCCATCGGGCGCTGGatgatg                                                                                                                               <  2:425503/150‑1 (MQ=255)
                           aCGGATCTTCCAGCAGGTTCTTGATAGTGACCAGGAAGGTTACCGCTTCCTTGCCGTCGATCAGGCGGTGATCGTACGACAGCGCCAGGTACATCATCGGGCGAATCACGACCTGGCCATTGATGGCCATCGGGCGCTGGATGAtgttgt                                                                                                                           <  1:493934/150‑1 (MQ=255)
                                            ttattGATAGTGACCAGGAAGGTTACCGCTTCCTTGCCGTCGATCAGGCGGTGATCGTACGACAGCGCCAGGTACATCATCGGGCGAATCACGACCTGGCCATGGATGGCCATCGGGCGCTGGATGATGTTGTGCATGCCGAGAATggcg                                                                                                          <  1:1202940/147‑1 (MQ=255)
                                            ttCTTGATAGTGACCAGGAAGGTTACCGCTTCCTTGCCGTCGATCAGGCGGTGATCGTACGACAGCGCCAGGTACATCATCGGGCGAATCACGACCTGGCCATTGATGGCCATCGGGCGCTGGATGATGTTGTGCATGCCGAGAATggcg                                                                                                          <  2:290461/150‑1 (MQ=255)
                                                 gATAGTGACCAGGAAGGTTACCGCTTCCTTGCCGTCGATCAGGCGGTGATCGTACGACAGCGCCAGGTACATCATCGGGCGAATCACGACCTGGCCATTGATGGCCATCGGGCGCTGGATGATGTTGTGCATGCCGAGAATGGCGGCCTg                                                                                                     >  1:479659/1‑150 (MQ=255)
                                                        aCCAGGAAGGTTACCGCTTCCTTGCCGTCGATCAGGCGGTGATCGTACGACAGCGCCAGGTACATCATCGGGCGAATCACGACCTGGCCATTGATGGCCATCGGGCGCTGGATGATGTTGTGCATGCCGAGAATGGCGGCCTGCGGCggg                                                                                              <  1:558601/150‑1 (MQ=255)
                                                         ccAGGAAGGTTACCGCTTCCTTGCCGTCGATCAGGCGGTGATCGTACGACAGCGCCAGGTACATCATCGGGCGAATCACGACCTGGCCATTGATGGCCATCGGGCGCTGGATGATGTTGTGCATGCCGAGAATGGCGGCCTGCGGCGGGt                                                                                             >  1:487526/1‑150 (MQ=255)
                                                         ccAGGAAGGTTACCGCTTCCTTGCCGTCGATCAGGCGGTGATCGTACGACAGCGCCAGGTACATCATCGGGCGAATCACGACCTGGCCATCAATGGCCag                                                                                                                                               <  1:992368/100‑2 (MQ=255)
                                                         ccAGGAAGGTTACCGCTTCCTTGCCGTCGATCAGGCGGTGATCGTACGACAGCGCCAGGTACATCATCGGGCGAATCACGACCTGGCCATCAATGGCCag                                                                                                                                               >  2:992368/1‑99 (MQ=255)
                                                             gAAGGTTACCGCTTCCTTGCCGTCGATCAGGCGGTGATCGTACGACAGCGCCAGGTACATCATCGGGCGAATCACGACCTGGCCATTGATGGCCATCGGGCGCTGGATGATGTTGTGCATGCCGAGAATGGCGGCCTGCGGCGGGTTGAc                                                                                         <  2:274683/150‑1 (MQ=255)
                                                                ggTTACCGCTTCCTTGCCGTCGATCAGGCGGTGATCGTACGACAGCGCCAGGTACATCATCGGGCGAATCACGACCTGGCCATTGATGGCCATCGGGCGCTGGATGATGTTGTGCATGCCGAGAATGGCGGCCTGCGGCGGGTTGAC‑GAt                                                                                     <  2:24397/150‑1 (MQ=255)
                                                                     ccGCTTCCTTGCCGTCGATCAGGCGGTGATCGTACGACAGCGCCAGGTACATCATCGGGCGAATCACGACCTGGCCATTGATGGCCATCGGGCGCTGGATGATGTTGTGCATGCCGAGAATGGCGGCCTGCGGCGGGTTGACAGATCgga                                                                                 >  1:1162744/1‑149 (MQ=255)
                                                                            cTTGCCGTCGATCAGCCGGAGAGGGCAGGGCGGCGCTAGGGACATCATAGGGCGAATCGCGACCTGGCCATTGATGGCCATCGGGCGCTGGATGATGTTGTGCATGCCGAGAATGGCGGCCTGCGGCGGGTTGAC‑GATCGGGGTCGAcat                                                                         <  2:152540/150‑1 (MQ=255)
                                                                                 cGTCGATCAGGCGGTGATCGTACGACAGCGCCAGGTACATCATCGGGCGAATCACGACCTGGCCATTGATGGCCATCGGGCGCTGGATGATGTTGTGCATGCCGAGAATGGCGGCCTGCGGCGGGTTGAC‑GATCGGGGTCGACATCATCg                                                                    >  2:287287/1‑150 (MQ=255)
                                                                                   tCGATCAGGCGGTGATCGTACGACAGCGCCAGGTACATCATCGGGCGAATCACGACCTGGCCATTGATGGCCATCGGGCAATCGATGCTCTTTTTCTGTTTGGGAATGGCGGCCTGCGGCGGGTTGAC‑GATCGGGGTCGACATCATCGaa                                                                  >  1:745045/1‑150 (MQ=255)
                                                                                         aGGCGGTGATCGTACGACAGCGCCAGGTACATCATCGGGCGAATCACGACCTGGCCATTGATGGCCATCGGGCGCTGGATGATGTTGTGCATGCCGAGAATGGCGGCCTGCGGCGGGTTGAC‑GATCGGGGTCGACATCATCGAACCGAAg                                                            >  1:748273/1‑150 (MQ=255)
                                                                                          ggCGGTGATCGTACGACAGCGCCAGGTACATCATCGGGCGAATCACGACCTGGCCATTGATGGCCATCGGGCGCTGGATGATGTTGTGCATGCCGAGAATGGCGGCCTGCGGCGGGTTGAC‑GATCGGGGTCGACATCATCGAACCGAAgg                                                           <  2:569341/150‑1 (MQ=255)
                                                                                           gCGGTGATCGTACGACAGCGCCAGGTACATCATCGGGCGAATCACGACCTGGCCATTGATGGCCATCGGGCGCTGGATGATGTTGTGCATGCCGAGAATGGCGGCCTGCGGCGGGTTGAC‑GATCGGGGTCGACATCATCGAACCGAAGGt                                                          >  1:1086899/1‑150 (MQ=255)
                                                                                                     tACGACAGCGCCAGGTACATCATCGGGCGAATCACGACCTGGCCATTGATGGCCATCGGGCGCTGGATGATGTTGTGCATGCCGAGAATGGCGGCCTGCGGCGGGTTGAC‑GATCGGGGTCGACATCATCGAACCGAAGGTGCCACCGTtg                                                >  2:330609/1‑150 (MQ=255)
                                                                                                     tACGACAGCGCCAGGTACATCATCGGGCGAATCACGACCTGGCCATTGATGGCCATCGGGCGCTGGATGATGTTGTGCATGCCGAGAATGGCGGCCTGCGGCGGGTTGAC‑GATCGGGGTCGACATCATCGAACCGAAGGTGCCACCGTtg                                                >  1:568120/1‑150 (MQ=255)
                                                                                                      aCGACAGCGCCAGGTACATCATCGGGCGAATCACGACCTGGCCATTGATGGCCATCGGGCGCTGGATGATGTTGTGCATGCCGAGAATGGCGGCCTGCGGCGGGTTGAC‑GATCGGGGTCGACATCATCGAACCGAAGGTGCCATCGTtgg                                               <  2:598956/150‑1 (MQ=255)
                                                                                                        gACAGCGCCAGGTACATCATCGGGCGAATCACGACCTGGCCATTGATGGCCATCGGGCGCTGGATGATGTTGTGCATGCCGAGAATggcgg                                                                                                         <  1:582366/91‑1 (MQ=255)
                                                                                                        gACAGCGCCAGGTACATCATCGGGCGAATCACGACCTGGCCATTGATGGCCATCGGGCGCTGGATGATGTTGTGCATGCCGAGAATggcgg                                                                                                         >  2:582366/1‑91 (MQ=255)
                                                                                                                 aGGTACATCATCGGGCGAATCACGACCTGGCCATTGATGGCCATCGGGCGCTGGATGATGTTGTGCATGCCGAGAATGTCGGCCTGCGGCGGGTTGAC‑GATCGGGGTCGACATCATCGAACCGAAGGTGCCACCGTTGGTGATGGTGAAg                                    <  1:787240/150‑1 (MQ=255)
                                                                                                                 aGGTACATCATCGGGCGAATCACGACCTGGCCATTGATGGCCATCGGGCGCTGGATGATGTTGTGCATGCCGAGAATGGCGGCCTGCGGCGGGTTGAC‑GATCGGGGTCGACATCATCGAACCGaa                                                             >  1:533159/1‑125 (MQ=255)
                                                                                                                 aGGTACATCATCGGGCGAATCACGACCTGGCCATTGATGGCCATCGGGCGCTGGATGATGTTGTGCATGCCGAGAATGGCGGCCTGCGGCGGGTTGAC‑GATCGGGGTCGACATCATCGAACCGaa                                                             <  2:533159/125‑1 (MQ=255)
                                                                                                                   gTACATCATCGGGCGAATCACGACCTGGCCATTGATGGCCATCGGGCGCTGGATGATGTTGTGCATGCCGAGAATGGCGGCCTGCGGCGGGTTGAC‑GATCGGGGTCGACATCATCGAACCGAAGGTGCCACCGTTGGTGATGGTGAAGGt                                  <  2:256801/150‑1 (MQ=255)
                                                                                                                   gTACATCATCGGGCGAATCACGACCTGGCCATTGATGGCCATCGGGCGCTGGATGATGTTGTGCATGCCGAGAATGGCGGCCTGCGGCGGGTTGAC‑GATCGGGGTCAACAGTGTCGAACCGAAGGTGCCACCGTTGGTGATGGTGAAGGt                                  >  1:918723/1‑150 (MQ=255)
                                                                                                                    tACATCATCGGGCGAATCACGACCTGGCCATTGATGGCCATCGGGCGCTGGATGATGTTGTGCATGCCGAGAATGGCGGCCTGCGGCGGGTTGAC‑GATCGGGGTCGACATCATCGAAc                                                                 <  1:935698/118‑1 (MQ=255)
                                                                                                                    tACATCATCGGGCGAATCACGACCTGGCCATTGATGGCCATCGGGCGCTGGATGATGTTGTGCATGCCGAGAATGGCGGCCTGCGGCGGGTTGAC‑GATCGGGGTCGACATCATCGAAc                                                                 >  2:935698/1‑118 (MQ=255)
                                                                                                                          atcGGGCGAATCACGACCTGGCCATTGATGGCCATCGGGCGCTGGATGATGTTGTGCATGCCGAGAATGGCGGCCTGCGGCGGGTTGAC‑GATCGGGGTCGACATCATCGAACCGAAGGTGCCACCGTTGGTGATGGTGAAGGTGCCACCg                           <  2:748273/150‑1 (MQ=255)
                                                                                                                                    tCACGACCTGGCCATTGATGGCCATCGGGCGCTGGATGATGTTGTGCATGCCGAGAATGGCGGCCTGCGGCGGGTTGAC‑GATCGGGGTCGACATCATCGAACCGAAGGTGCCACCGTTGGTGATGGTGAAGGTGCCACCGGTCATCTCtt                 <  2:487526/150‑1 (MQ=255)
                                                                                                                                    tCACGACCTGGCCATTGATGGCCATCGGGCGCTGGATGATGTTGTGCATGCCGAGAATGGCGGCCTGCGGCGGGTTGAC‑GATCGGGGTCGACATCATCGAACCGAAGGTGCCACCGTTGGTGATGGTGAAGGTGCCACCGGTCATCTCtt                 <  1:279063/150‑1 (MQ=255)
                                                                                                                                     cACGACCTGGCCATTGATGGCCATCGGGCGCTGGATGATGTTGTGCATGCCGAGAATGGCGGCCTGCGGCGGGTTGAC‑GATCGGGGTCGACATCATCGAACCGAAGGTGCCACCGTTGGTGATGGTGAAGGTGCCACCGGTCATCTCTTc                >  1:1055224/1‑150 (MQ=255)
                                                                                                                                       cGACCTGGCCATTGATGGCCATCGGGCGCTGGATGATGTTGTGCATGCCGAGAATGGCGGCCTGCGGCGGGTTGAC‑GATCGGGGTCGACATCATCGAACCGAAGGTGCCACCGTTGGTGATGGTGAAGGTGCCACCGGTCATCTCTTCGa              <  2:1086899/150‑1 (MQ=255)
                                                                                                                                         aCCTGGCCATTGATGGCCATCGGGCGCTGGATGATGTTGTGCATGCCGAGAATGGCGGCCTGCGGCGGGTTGAC‑GATCGGGGTCGACATCATCGAACCGAAGGTGCCACCGTTGGTGATGGTGAAGGTGCCACCGGTCATCTCTTCGATg            <  1:330609/150‑1 (MQ=255)
                                                                                                                                          gatGGCCATCAATGGCCATCGGGCGCTGGATGATGTTGTGCATGCCGAGAATGGCGGCCTGCGGCGGGTTGAC‑GATCGGGGTCGACATCATCGAACCGAAGGTGCCACCGTTGGTGATGGTGAAg                                    <  1:320311/123‑1 (MQ=255)
                                                                                                                                          gatGGCCATCAATGGCCATCGGGCGCTGGATGATGTTGTGCATGCCGAGAATGGCGGCCTGCGGCGGGTTGAC‑GATCGGGGTCGACATCATCGAACCGAAGGTGCCACCGTTGGTGATGGTGAAg                                    >  2:320311/3‑125 (MQ=255)
                                                                                                                                           cTGGCCATCGATCGCCCTCGCGCGCTGGTTGGTGCTCGGGTGTCGGCGAAGGGCGGCCTGCGGCGGGTTGAC‑GATCGGGGTCGACATCATCGAACCGAAGGTGCCACCGTTGGTGATGGTGAAGGTGTCACCGGTCATCTCTTCGATGGa          <  2:918723/150‑1 (MQ=255)
                                                                                                                                                cATTGATGGCCATCGGGCGCTGGATGATGTTGTGCATGCCGAGAATGGCGGCCTGCGGCGGGTTGAC‑GATCGGGGTCGACATCATCGAACCGAAGGTGCCACCGTTGGTGATGGTGAAGGTGCCACCGGTCATCTCTTCGATGGACAGtt     >  1:603754/1‑150 (MQ=255)
                                                                                                                                                cATAGATGGCCATCGGGCTCTGGATGATGTTGTGCATGCCGAGAATGGCGGCCTGCGGCGGGTTGAC‑GGTCGGGGTCGGCATCCTCGAACCGAAGGTTCCACCGTTGGAGTTGGTGCAACCGCCACCGGGCATCGATTCGAGAGACAGtt     >  2:965286/1‑150 (MQ=255)
                                                                                                                                                   tGATGGCCATCGGGCGCTGGATGATGTTGTGCATGCCGAGAATGGCGGCCTGCGGCGGGTTGAC‑GATCGGGGTCGACATCATCGAACCGAAGGTGCCACCGTTGGTGATGGTGAAGGTGCCACCGGTCATCTCTTCGATGGACAGTTTGc  <  2:809400/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                      
AGGGTTAGATGTCCAGCAGCAGGCGGGACGGATCTTCCAGCAGGTTCTTGATAGTGACCAGGAAGGTTACCGCTTCCTTGCCGTCGATCAGGCGGTGATCGTACGACAGCGCCAGGTACATCATCGGGCGAATCACGACCTGGCCATTGATGGCCATCGGGCGCTGGATGATGTTGTGCATGCCGAGAATGGCGGCCTGCGGCGGGTTGAC‑GATCGGGGTCGACATCATCGAACCGAAGGTGCCACCGTTGGTGATGGTGAAGGTGCCACCGGTCATCTCTTCGATGGACAGTTTGC  >  NC_002947/4731579‑4731875

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑