Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,768,309 T→G 10.2% E183A (GAG→GCG)  PP_4218 ← lipase/esterase family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,768,3090TG10.2% 124.7 / 6.7 49E183A (GAG→GCG) PP_4218lipase/esterase family protein
Reads supporting (aligned to +/- strand):  ref base T (23/21);  new base G (2/3);  total (25/24)
Fisher's exact test for biased strand distribution p-value = 6.67e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.78e-01

GGCACCGTGGCGTATTGCTGGTAGAACCACTCCAGGGTCTGCGCTTCAAGCAGGTAACCGCTGCCATAGCGCTGCACCGAAGGCCGCCGGCAACTGGCATCGGTCACCGGGTAGATCATTACCTGCAGGCGCGGTGCCGGCAGCTCGCGCTGCGCAGCCAACTGGTTGGCCAGAATGGTAGCCAGGCTACCACCGACACTGTCGCCCACCACCGCCAGTCGCTGCGCATCGATGCCCAGCGCCTCGGCCTGCTCGACCAACCAGCGCCAGGCATCCAGGGCATCGTCACTGGCT  >  NC_002947/4768165‑4768458
                                                                                                                                                |                                                                                                                                                     
ggCACCGTGGCGTATTGCTGGTAGAACCACTCCAGGGTCTGCGCTTCAAGCAGGTAACCGCTGCCATAGCGCTGCACCGAAGGCCGCCGGCAACTGGCATCGGTCACCGGGTAGATCATTACCTGCAGGCGCGGTGCCGGCAGCTCgcgc                                                                                                                                                  >  1:969100/1‑150 (MQ=255)
    ccGTGGCGTATTGCTAGTAGAACCACTCCAGGGTCTGCGCTTCAAGCAGGTAACCGCTGCCATAGCGCTGCACCGAAGGCCGCCGGCAACTGGCATCGGTCACCGGGTAGATCATTACCTGCAGGCGCGGTGCCGGCAGCGCGAGCTgcc                                                                                                                                              <  2:1112897/150‑2 (MQ=255)
                  tGGTCGAACCCCTCCAGGGTCTGCGCTTCAAGCAGGTAACCGCTGCCATAGCGCTGCACCGAAGGCCGCCGGCAACTGGCATCGGTCACCGGGTAGATCATTACCTGCAGGCGCGGTGCCGGCAGCTCGCGCTGCGCAGCCAACTGGTTg                                                                                                                                <  1:474543/150‑1 (MQ=255)
                    gTAGAACCACTCCAGGGTCTGCGCTTCAAGCAGGTAACCGCTGCCATAGCGCTGCACCGAAGGCCGCCGGCAACTGGCATCGGTCACCGGGTAGATCATTACCTGCAGGCGCGGTGCCGGCAGCTCGCGCTGCGCAGCCAACTGGTTGGc                                                                                                                              >  2:591329/1‑150 (MQ=255)
                         aCCACTCCAGGGTCTGCGCTTCAAGCAGGTAACCGCTGCCATAGCGCTGCACCGAAGGCCGCCGGCAACTGGCATCGGTCACCGGGTAGATCATTACCTGCAGGCGCGGTGCCGGCAGCTCGCGCTGCGCAGCCAACTGGTTGGCCAGaa                                                                                                                         >  2:56088/1‑150 (MQ=255)
                         aCCACTCCAGGGTCTGCGCTTCAAGCAGGTAACCGCTGACATAGCGCTGCACCGAAGGCCGCCGGCAACTGGCATCGGTCACCGGGTAGATCATTACCTGCAGGCGCGGTGCCGGCAGCTCGCGCTGCGCAGCCAACTGGTTGGCCAGaa                                                                                                                         <  1:63799/150‑1 (MQ=255)
                           cACTCCAGGGTCTGCGCTTCAAGCAGGTAACCGCTGCCATAGCGCTGCACCGAAGGCCGCCGGCAACTGGCATCGGTCACCGGGTAGATCATTACCTGCAGGCGCGGTGCCGGCAGCTCGCGCTGCGCAGCCAACTGGTTGGCCAGAATg                                                                                                                       >  2:1126374/1‑150 (MQ=255)
                            aCTCCAGGGTCTGCGCTTCAAGCAGGTAACCGCTGCCATAGCGCTGCACCGAAGGCCGCCGGCAACTGGCATCGGTCACCGGGTAGATCATTACCTGCAGGCGCGGTGCCGGCAGCTCGCGCTGCGCAGCCAACTGGTTGGCCAGAATgg                                                                                                                      <  1:1000487/150‑1 (MQ=255)
                            aCTCCAGGGTCTGCGCTTCAAGCAGGTAACCGCTACGATAGCGCTGCACCGAAGGCCGCCGGCAACTGGCATCGGTCCCCGGGTAGATCATTACCTGCAGGCGCGGTGCCGGCAGCTCGCGCTGCGCAGCCAACTGGTTGGCCAGAATgg                                                                                                                      >  1:401444/1‑150 (MQ=255)
                              tCCAGGGTCTGCGCTTCAAGCAGGTAACCGCTGCCATAGCGCTGCACCGAAGGCCGCCGGCAACTGGCATCGGTCACCGGGTAGATCATTACCTGCAGGCGCGGTGCCGGCAGCTCGCGCTGCGCAGCCAACTGGTTGGCCAGAATGGTa                                                                                                                    <  1:366592/150‑1 (MQ=255)
                                  gggTCTGCGCTTCAAGCAGGTAACCGCTGCCATAGCGCTGCACCGAAGGCCGCCGGCAACTGGCATCGGTCACCGGGTAGATCATTACCTGCAGGCGCGGTGCCGGCAGCTCGCGCTGCGCAGCCAACTGGTTGGCCAGAATGGTAGCCa                                                                                                                <  2:387847/150‑1 (MQ=255)
                                           cTTCAAGCAGGTAACCGCTGCCATAGCGCTGCACCGAAGGCCGCCGGCAACTGGCATCGGTCACCGGGTAGATCATTACCTGCAGGCGCGGTGCCGGCAGCTCGCGCTGCGCAGCCAACTGGTTGGCCAGAATGGTAGCCAGGCTaccac                                                                                                       >  2:948730/1‑150 (MQ=255)
                                           cTTCAAGCAGGTAACCGCTGCCATAGCGCTGCACCGAAGGCCGCCGGCAACTGGCATCGGTCACCGGGTAGATCATTACCTGCAGGCGCGGTGCCGGCAGCGCGAGCTgcc                                                                                                                                              <  1:606860/111‑2 (MQ=255)
                                           cTTCAAGCAGGTAACCGCTGCCATAGCGCTGCACCGAAGGCCGCCGGCAACTGGCATCGGTCACCGGGTAGATCATTACCTGCAGGCGCGGTGCCGGCAGCGCGAGCTgcc                                                                                                                                              >  2:606860/1‑110 (MQ=255)
                                              cAAGCAGGTAACCGCTGCCATAGCGCTGCACCGAAGGGGGCCGGCAACTGGCATCGGTCACCGGGTAGATCATTACCTGCAGGCGCGGTGCCGGCAGCTCGCGCTGCGCAGCCAACTGGTTGGCCAGAATGGTAGCCAGGCTACCACCGa                                                                                                    <  2:401444/150‑1 (MQ=255)
                                                     gTAACCGCTGCCATAGCGCTGCACCGAAGGCCGCCGGCAACTGGCATCGGTCACCGGGTAGATCATTACCTGCAGGCGCGGTGCCGGCAGCTCGCGCTGCGCAGCCAACTGGTTGGCCAGAATGGTAGCCAGGCTACCACCGACACTGTc                                                                                             <  1:591329/150‑1 (MQ=255)
                                                       aaCCGCTGCCATAGCGCTGCACCGAAGGCCGCCGGCAACTGGCATCGGTCACCGGGTAGATCATTACCTGCAGGCGCGGTGCCGGCAGCTCGCGCTGCGCAGCCAACTGGTTGGCCAGAATGGTAGCCAGGCTacc                                                                                                         >  1:343824/1‑136 (MQ=255)
                                                       aaCCGCTGCCATAGCGCTGCACCGAAGGCCGCCGGCAACTGGCATCGGTCACCGGGTAGATCATTACCTGCAGGCGCGGTGCCGGCAGCTCGCGCTGCGCAGCCAACTGGTTGGCCAGAATGGTAGCCAGGCTacc                                                                                                         <  2:343824/136‑1 (MQ=255)
                                                              gCCATAGCGCTGCACCGAAGGCCGCCGGCAACTGGCATCGGTCACCGGGTAGATCATTACCTGCAGGCGCGGTGCCGGCAGCTCGCGCTGCGCAGCCAACTGGTTGGCCAGAATGGTAGCCAGGCTACCACCGACACTGTCGCCcaccac                                                                                    <  2:157378/150‑1 (MQ=255)
                                                               ccATAGCGCTGCACCGAAGGCCGCCGGCAACTGGCATCGGTCACCGGGTAGATCATTACCTGCAGGCGCGGTGCCGGCAGCTCGCGCTGCGCAGCCAACTGGTTGGCCAGAATGGTAGCCAGGCTACCACCGACACTGTc                                                                                             >  1:788758/1‑140 (MQ=255)
                                                               ccATAGCGCTGCACCGAAGGCCGCCGGCAACTGGCATCGGTCACCGGGTAGATCATTACCTGCAGGCGCGGTGCCGGCAGCTCGCGCTGCGCAGCCAACTGGTTGGCCAGAATGGTAGCCAGGCTACCACCGACACTGTc                                                                                             <  2:788758/140‑1 (MQ=255)
                                                                 aTAGCGCTGCACCGAAGGCCGCCGGCAACTGGCATCGGTCACCGGGTAGATCATTACCTGCAGGCGCGGTGCCGGCAGCTCGCGCTGCGCAGCCAACTGGTTGGCCAGAATGGTAGCCAGGCTACCACCGACACTGTCGCCCACCACCGc                                                                                 <  2:695756/150‑1 (MQ=255)
                                                                           aCCGAAGGCCGCCGGCAACTGGCATCGGTCACCGGGTAGATCATTACCTGCAGGCGCGGTGCCGGCAGCTCGCGCTGCGCAGCCAACTGGTTGGCCAGAATGGTAGCCAGGCTACCACCGACACTGTCGCCCACCACCGc                                                                                 <  1:214485/140‑1 (MQ=255)
                                                                           aCCGAAGGCCGCCGGCAACTGGCATCGGTCACCGGGTAGATCATTACCTGCAGGCGCGGTGCCGGCAGCTCGCGCTGCGCAGCCAACTGGTTGGCCAGAATGGTAGCCAGGCTACCACCGACACTGTCGCCCACCACCGc                                                                                 >  2:214485/1‑140 (MQ=255)
                                                                                     gccgGCAACTGGCATCGGTCACCGGGTAGATCATTACCTGCAGGCGCGGTGCCGGCAGCTCGCGCTGCGCAGCCAACTGGTTGGCCAGAATGGTAGCCAGGCTACCACCGACACTGTCGCCCACCACCGCCAGTCGCTGCGCATCGATGc                                                             >  1:40797/1‑150 (MQ=255)
                                                                                          cAACTGGCATCGGTCACCGGGTAGATCATTACCTGCAGGCGCGGTGCCGGCAGCTCGCGCTGCGCAGCCAACTGGTTGGCCAGAATGGTAGCCAGGCTacca                                                                                                        >  2:220569/1‑102 (MQ=255)
                                                                                          cAACTGGCATCGGTCACCGGGTAGATCATTACCTGCAGGCGCGGTGCCGGCAGCGCTCGCTGCGCAGCCAACTGGTTGGCCAGAATGGTAGCCAGGCTacca                                                                                                        <  1:220569/102‑1 (MQ=255)
                                                                                                  aTCGGTCACCGGGTAGATCATTACCTGCAGGCGCGGTGCCGGCAGCTCGCGCTGCGCAGCCAACTGGTTGGCCAGAATGGTAGCCAGGCTACCACCGACACTGTCGCCCACCACCGCCAGTc                                                                            >  2:624577/1‑122 (MQ=255)
                                                                                                  aTCGGTCACCGGGTAGATCATTACCTGCAGGCGCGGTGCCGGCAGCTCGCGCTGCGCAGCCAACTGGTTGGCCAGAATGGTAGCCAGGCTACCACCGACACTGTCGCCCACCACCGCCAGTc                                                                            <  1:624577/122‑1 (MQ=255)
                                                                                                  aTCGGTCACCGGGTAGATCATTACCTGCAGGCGCGGTGCCGGCAGCTCGCGCTGCGCAGCCAACTGGTTGGCCAGAATGGTAGCCAGGCTACCACCGACACTGTCGCCCACCACCGCCAGTCGCTGCGCATCGATGCCCAGCGCCTCGGc                                                >  2:698303/1‑150 (MQ=255)
                                                                                                    cGGTCACCGGGTAGATCATTACCTGCAGGCGCGGTGCCGGCAGCTCGCGCTGCGCAGCCAACTGGTTGGCCAGAATGGTAGCCAGGCTACCACCGACACTGTCGCCCACCACCGCCAGTCGCTGCGCATCGATGCCCAGCGCCTCGGCCt                                              >  1:1112035/1‑150 (MQ=255)
                                                                                                             ggTAGATCATTACCTGCAGGCGCGGTGCCGGCAGCTCGCGCTGCGCAGCCAACTGGTTGGCCAGaa                                                                                                                         <  2:918166/66‑1 (MQ=255)
                                                                                                             ggTAGATCATTACCTGCAGGCGCGGTGCCGGCAGCTCGCGCTGCGCAGCCAACTGGTTGGCCAGaa                                                                                                                         >  1:918166/1‑66 (MQ=255)
                                                                                                              gTAGATCATTACCTGCAGGCGCGGTGCCGGCAGCTCGCGCTGCGCAGCCAACTGGTTGGCCAGAATGGTAGCCAGGCTACCACCGACACTGTCGcc                                                                                          >  2:1008553/1‑96 (MQ=255)
                                                                                                              gTAGATCATTACCTGCAGGCGCGGTGCCGGCAGCTCGCGCTGCGCAGCCAACTGGTTGGCCAGAATGGTAGCCAGGCTACCACCGACACTGTCGcc                                                                                          <  1:1008553/96‑1 (MQ=255)
                                                                                                                aGATCATTACCTGCAGGCGCGGTGCCGGCAGCTCGCGCTGCGCAGCCAACTGGTTGGCCAGAATGGTAGCCAGGCTACCACCGACACTGTCGCCCACCACCGCCAGTCGCTGCGCATCGATGCCCAGCGCCTCGGCCTGCTCGaccaacc                                  >  2:345590/1‑150 (MQ=255)
                                                                                                                   tCATTACCTGCAGGCGCGGTGCCGGCAGCTCGCGCTGCGCAGCCAACTGGTTGGCCAGAATGGTAGCCAGGCTACCACCGACACTGTCGCCCACCACCGCCAGTCGCTGCGCATCGATGCCCAGCGCCTCGGCCTGCTCGACCAACCAgc                               >  2:973027/1‑150 (MQ=255)
                                                                                                                     aTTGCCTGCAGGCGCGGTGCCGGCAGCTCGCGCTGCGCAGCCAACTGGTTGGCCAGAATGGTAGCCAGGCTACCACCGACACTGTCGCCCACCAACGCCAGTCGCTGCGCATCGATGCCCAGCGCCTCGGCCTGCTCGATGAGCCAgcgg                             >  1:956735/1‑149 (MQ=255)
                                                                                                                     aTTACCTGCAGGCGCGGTGCCGGCAGCTCGCGCTGCGCAGCCAACTGGTTGGCCAGAATGGTAGCCAGGCTACCACCGACACTGTCGCCCACCACCGCCAGTCGCTGCGCATCGATGCCCAGCGCCTCGGCCTGCTCGACCAACCAgcgc                             <  1:973027/150‑1 (MQ=255)
                                                                                                                       tACCTGCAGGCGCGGTGCCGGCAGCTCGCGCTGCGCAGCCAACTGGTTGGCCAGAATGGTAGCCAGGCTACCACCGACACTGTCGCCCACCACCGCCAGTCGCTGCGCATCGATGCCCAGCGCCTCGGCCTGCTCGACCAACCAGCGCCa                           >  2:1062903/1‑150 (MQ=255)
                                                                                                                        aCCTGCAGGCGCGGTGCCGGCAGCTCGCGCTGCGCAGCCAACTGGTTGGCCAGAATGGTAGCCAGGCTACCACCGACACTGTCGCCCACCACCGCCAGTCGCTGCGCATCGATGCCCAGCGCCTCGGCCTGCTCGACCAACCAGCGCCAg                          <  2:1112035/150‑1 (MQ=255)
                                                                                                                           tGCAGGCGCGGTGCCGGCAGCTCGCGCTGCGCAGCCAACTGGTTGGCCAGAATGGTAGCCAGGCTACCACCGACACTGTCGCCCACCACCGCCAGTCGCTGCGCATCGATGCCCAGCGCCTCGGCCTGCTCGACCAACCAGCGCCAGGCa                       >  1:18751/1‑150 (MQ=255)
                                                                                                                               ggCGCGGTGCCGGCAGCTCGCGCTGCGCAGCCAACTGGTTGGCCAGAATGGTAGCCAGGCTACCACCGACACTGTCGCCCACCACCGCCAGTCGCTGCGCATCGATGCCCAGCGCCTCGGCCTGCTCGACCAACCAGCGCCAGGCATCCa                   >  1:578486/1‑150 (MQ=255)
                                                                                                                                gcgcGGTGCCGGCAGCTCGCGCTGCGCAGCCAACTGGTTGGCCAGAATGGTAGCCAGGCTACCACCGACACTGTCGCCCACCACCGCCAGTCGCTGCGCATCGATGCCCAGCGCCTCGGCCTGCTCGACCAACCAGCGCCAGGCATCCAg                  <  1:1126374/150‑1 (MQ=255)
                                                                                                                                     gTGCCGGCAGCTCGCGCTGCGCAGCCAACTGGTTGGCCAGAATGGTAGCCAGGCTACCACCGACACTGTCGCCCACCACCGCCAGTCGCTGCGCATCGATGCCCAGCGCCTCGGCCTGCTCGACCAACCAGGGCCAGGCATCCAGGGCAt             >  2:167649/1‑150 (MQ=255)
                                                                                                                                      tGCCGGCAGCTCGCGCTGCGCAGCCAACTGGTTGGCCAGAATGGTAGCCAGGCTACCACCGACACTGTCGCCCACCACCGCCAGTCGCTGCGCATCGATGCCCAGCGCCTCGGCCTGCTCGACCAACCAGGGCCAGGCATCCAGGGCATc            <  1:167649/150‑1 (MQ=255)
                                                                                                                                        tgcgCAGCGCGAGCTGCGCAGCCAACTGGTTGGCCAGAATGGTAGCCAGGCTACCACCGACACTGTCGCCCACCACCGCCAGTCGCTGCGCATCGATGCCCAGCGCCTCGGCCTGCTCGACCAACCAGCGCCAGGCATCCAGGGCATCGt          >  2:827141/4‑150 (MQ=255)
                                                                                                                                             aGCTCGCGCTGCGCAGCCAACTGGTTGGCCAGAATGGTAGCCAGGCTACCACCGACACTGTCGCCCACCACCGCCAGTCGCTGCGCATCGATGCCCAGCGCCTCGGCCTGCTCGACCAACCAGCGCCAGGCATCCAGGGCATCGTCACTg     <  2:40797/150‑1 (MQ=255)
                                                                                                                                             aGCTCGCGCTGCGCAGCCAACTGGTTGGCCAGAATGGTAGCCAGGCTACCACCGACACTGTCGCCCACCACCGCCAGTCGCTGCGCATCGATGCCCAGCGCCTCGGCCTGCTCGACCAACCAGCGCCAGGCATCCAGGGCATCGTCACTg     <  1:345590/150‑1 (MQ=255)
                                                                                                                                                tCGCGCTGCGCAGCCAACTGGTTGGCCAGAATGGTAGCCAGGCTACCACCGACACTGTCGCCCACCACCGCCAGTCGCTGCGCATCGATGCCCAGCGCCTCGGCCTGCTCGACCAACCAGCGCCAGGCATCCAGGGCATCGTCACTGGCt  >  2:294201/1‑150 (MQ=255)
                                                                                                                                                |                                                                                                                                                     
GGCACCGTGGCGTATTGCTGGTAGAACCACTCCAGGGTCTGCGCTTCAAGCAGGTAACCGCTGCCATAGCGCTGCACCGAAGGCCGCCGGCAACTGGCATCGGTCACCGGGTAGATCATTACCTGCAGGCGCGGTGCCGGCAGCTCGCGCTGCGCAGCCAACTGGTTGGCCAGAATGGTAGCCAGGCTACCACCGACACTGTCGCCCACCACCGCCAGTCGCTGCGCATCGATGCCCAGCGCCTCGGCCTGCTCGACCAACCAGCGCCAGGCATCCAGGGCATCGTCACTGGCT  >  NC_002947/4768165‑4768458

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑