Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,840,256 G→T 8.1% A193S (GCC→TCC)  ccoN‑II → cbb3‑type cytochrome c oxidase subunit 1

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,840,2560GT 8.1% 150.5 / 2.2 49A193S (GCC→TCC) ccoN‑IIcbb3‑type cytochrome c oxidase subunit 1
Reads supporting (aligned to +/- strand):  ref base G (27/18);  new base T (2/2);  total (29/20)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.78e-01

CTGGTGTTCTTCGGCACTATCGTCAAACGCAAGACCAAGCACATCTATGTCGGCAACTGGTTCTACGGTGCGTTCATCGTGGTCACCGCGATGCTGCACATCGTCAACCACATCTCGCTGCCGGTGAGCCTGTTCAAGTCGTACTCGGCCTACTCCGGCGCCACCGACGCGATGATCCAGTGGTGGT‑‑ACGGCCACAACGCCGTGGGCTTCTTCCTCACCACCGGCTTCCTGGGGATGATGTACTACTTCGTGCCCAAGCAGGCCGAGCGGCCGATCTACTCGTATCGCTTGTC  >  NC_002947/4840109‑4840401
                                                                                                                                                   |                                                                                                                                                   
cTGGTGTTCTTCGGCACTATCGTCAAACGCAAGACCAAGTACATCTATGTCGGCAACTGGTTCTACGGTGCGTTCATCGTGGTCACCGCGATGCTGCACATCGTCAACCACACCTCGCTGCCGGTGAGCCTGTTCAAGTCGTACTCGGcc                                                                                                                                                   <  2:121417/150‑1 (MQ=255)
     gtTCTTCGGCACTATCGTCAAACGCAAGACCAAGCACATCTATGTCGGCAACTGGTTCTACGGTGCGTTCATCGTGGTCACCGCGATGCTGCACATCGTCAACCACATCTCGCTGCCGGTGAGCCTGTTCAAGTCGTACTCGGCCTACTc                                                                                                                                              >  1:371041/1‑150 (MQ=255)
      ttcttcGGCACTATCGTCAAACGCAAGACCAAGCACATCTATGTCGGCAACTGGTTCTACGGTGCGTTCATCGTGGTCACCGCGATGCTGCACATCGTCAACCACATCTCGCTGCCGGTGAGCCTGTTCAAGTCGTACTCGGCCTACTcc                                                                                                                                             >  2:231215/1‑150 (MQ=255)
         ttcGGCACTATCGTCAAACGCAAGACCAAGCACATCTATGTCGGCAACTGGTTCTACGGTGCGTTCATCGTGGTCACCGCGATGCTGCACATCGTCAACCACATCTCGCTGCCGGTGAGCCTGTTCAAGTCGTACTCGGCCTACTCCGgc                                                                                                                                          <  2:1192877/150‑1 (MQ=255)
         ttcGGCACTATCGTCAAACGCAAGACCAAGCACATCTATGTCGGCAACTGGTTCTACGGTGCGTTCATCGTGGTCACCGCGATGCTGCACATCGTCAACCACATCTCGCTGCCGGTGAGCCTGTTCAAGTCGTACTCGGCCTACTCCGgc                                                                                                                                          >  1:1192877/1‑150 (MQ=255)
                      tCAAACGCAAGACCAAGCACATCTATGTCGGCAACTGGTTCTACGGTGCGTTCATCGTGGTCACCGCGATGCTGCACATCGTCAACCACATCTCGCTGCCGGTGAGCCTGTTCAAGTCGTACTCGGCCTACTCCGGCGCCACCGACGCga                                                                                                                             >  2:186054/1‑150 (MQ=255)
                      tCAAACGCAAGACCAAGCACATCTATGTCGGCAACTGGTTCTACGGTGCGTTCATCGTGGTCACCGCGATGCTGCACATCGTCAACCACATCTCGCTGCCGGTGAGCCTGTTCAAGTCGTACTCGGCCTACTCCGGCGCCACCGACGCga                                                                                                                             >  2:343925/1‑150 (MQ=255)
                            gCAAGACCAAGCACATCTATGTCGGCAACTGGTTCTACGGTGCGTTCATCGTGGTCACCGCGATGCTGCACATCGTCAACCACATCTCGCTGCCGGTGAGCCTGTTCAAGTCGTACTCGGCCTACTCCGGCGCCACCGCCGCGATGATcc                                                                                                                       >  1:533108/1‑150 (MQ=255)
                                   cAAGCACATCTATGTCGGCAACTGGTTCTACGGTGCGTTCATCGTGGTCACCGCGATGCTGCACATCGTCAACCACATCTCGCTGCCGGTGAGCCTGTTCAAGTCGTACTCGTCCTACTCCGGCGCCACCGACGCGATGATCCAgtggtg                                                                                                                >  1:617305/1‑150 (MQ=255)
                                   cAAGCACATCTATGTCGGCAACTGGTTCTACGGTGCGTTCATCGTGGTCACCGCGATGCTGCACATCGTCAACCACATCTCGCTGCCGGTGAGCCTGTTCAAGTCGTACTCGGCCTACTCCGGCGCCACCGACGCGATGATCCAgtggtg                                                                                                                >  1:826132/1‑150 (MQ=255)
                                    aaGCACATCTATGTCGGCAACTGGTTCTACGGTGCGTTCATCGTGGTCACCGCGATGCTGCACATCGTCAACCACATCTCGCTGCCGGTGAGCCTGTTCAAGTCGTACTCGGCCTACTCCGGCGCCACCGACGCGATGATCCAGtggtgg                                                                                                               >  2:248677/1‑150 (MQ=255)
                                              aTGTCGGCAACTGGTTCTACGGTGCGTTCATCGTGGTCACCGCGATGCTGCACATCAACAACCACATCTCGCTGCCGGTGAGCCTGTTCAAGTCGTACTCGTCCTACTCCGGCGCCACCGACGCGATGATCCAGTGGTGGT‑‑ACGGCcaca                                                                                                   >  2:466258/1‑150 (MQ=255)
                                                   ggCAACTGGTTCTACGGTTCGTTCATCGTGGTCACAACAATTCGACACATCGTAAACCACATCTCGCTGCCGGTGAGCCTGTTCAAGTCGTACTCCGCCTACTCCGGCCCCACCGACGCGATGATCCAGTGGTGGT‑‑ACGGCCACAACGcc                                                                                              >  2:42025/1‑150 (MQ=255)
                                                      aaCTGGTTCTACGGTGCGTTCATCGTGGTCACCGCGATGCTGCACATCGTCAACCACATCTCGCTGCCGGTGAGCCTGTTCAAGTCGTACTCGGCCTACTCCGGCGCCACCGACGCGATGATCCAGTGGTGGT‑‑ACGGCCACAACGCCGTg                                                                                           <  2:371041/150‑1 (MQ=255)
                                                       aCTGGTTCTACGGTGCGTTCATCGTGGTCACCGCGATGCTGCACATCGTCAACCACATCTCGCTGCCGGTGAGCCTGTTCAAGTCGTACTCGGCCTACTCCGGCGCCACCGACGCGATGATCCAgtggtg                                                                                                                >  1:268237/1‑130 (MQ=255)
                                                       aCTGGTTCTACGGTGCGTTCATCGTGGTCACCGCGATGCTGCACATCGTCAACCACATCTCGCTGCCGGTGAGCCTGTTCAAGTCGTACTCGGCCTACTCCGGCGCCACCGACGCGATGATCCAgtggtg                                                                                                                <  2:268237/130‑1 (MQ=255)
                                                         tGGTTCTACGGTGCGTTCATCGTGGTCACCGCGATGCTGCACATCGTCAACCACATCTCGCTGCCGGTGAGCCTGTTCAAGTCGTACTCGGCCTACTCCGGCGCCACCGACGCGATGATCCAGTGGTGGT‑‑ACGGCCACAACGCCGTGGGc                                                                                        <  2:998310/150‑1 (MQ=255)
                                                           gTTCTACGGTGCGTTCATCGTGGTCACCGCGATGCTGCACATCGTCAACCACATCTCGCTGCCGGTGAGCCTGTTCAAGTCGTACTCGGCCTACTCCGGCGCCACCGACGCGATGATCCAGTGGTGGT‑‑ACGGCCACAACGCCGTGGGctt                                                                                      >  1:1042829/1‑150 (MQ=255)
                                                               tACGGTGCGTTCATCGTGGTCACCGCGATGCTGCACATCGTCAACCACATCTCGCTGCCGGTGAGCCTGTTCAAGTCGTACTCGGCCTACTCCGGCGCCACCGACGCGATGATCCAGTGGTGGT‑‑ACGGCCACAACGCCGTGGGCttcttc                                                                                  <  1:777386/150‑1 (MQ=255)
                                                                       gTTCATCGTGGTCACCGCGATGCTGCACATCGTCAACCACATCTCGCTGCCGGTGAGACTGTTCAAGTCGTACTCGGCCTACTACGGCGCCACCGACGCGCTGATCCAGTGGTGGT‑‑CCGCACACAACGCCGTGGGCTTCTTACTcaccac                                                                          >  2:532073/1‑150 (MQ=255)
                                                                        ttCATCGTGGTCACCGCGATGCTGCACATCGTCAACCACATCTCGCTGCCGGTGAGCCTGTTCAAGTCGTACTCGGCCTACTCCGGCGCCACCGACGCGATGATCCAGTGGTGGT‑‑ACGGCCACAACGCCGTGGGCTTCTTCCTCaccacc                                                                         <  1:560934/150‑1 (MQ=255)
                                                                         tCATCGTGGTCACCGCGATGCTGCACATCGTCAACCACATCTCGCTGCCGGTGAGCCTGTTCAAGTCGTACTCGGCCTACTCCGGCGCCACCGACGCGATGATCCAGTGGTGGT‑‑ACGGCCACAACGCCGTGGGCTTCTTCCTCACCACCg                                                                        <  1:186054/150‑1 (MQ=255)
                                                                          cATCGTGGTCACCGCGATGCTGCACATCGTCAACCACATCTCGCTGCCGGTGAGCCTGTTCAAGTCGTACTCGGCCTACTCCGGCGCCACCGACGCGATGATCCAGTGGTGGT‑‑ACGGCCACAACGCCGTGGGCTTCTTCCTCACCACCgg                                                                       <  1:343925/150‑1 (MQ=255)
                                                                                    aCCGCGATGCTGCACATCGTCAACCACATCTCGCTGCCGGTGAGCCTGTTCAAGTCGTACTCGGCCTACTCCGGCGCCACCGACGCGATGATCCAGTGGTGGT‑‑ACGGCCACAACGCCGTGGGCTTCTTCCTCACCACCGGCTTCCTgggg                                                             >  2:382842/1‑150 (MQ=255)
                                                                                    aCCGCGATGCTGCACATCGTCAACCACATCTCGCTGCCGGTGAGCCTGTTCAAGTCGTACTCGGCCTACTCCGGCGCCACCGACGCGATGATCCAGTGGTGGT‑‑ACGGCCACAACGCCGTGGGCTTCTTCCTCACCACCGGCTTCCTgggg                                                             >  1:952533/1‑150 (MQ=255)
                                                                                     ccGCGATGCTGAACATCGTCAACCACATCTCGCTGCCGGTGAGCCTGTTCAAGTCGTACTCGGCCTACTCCGGCGCCACCGACGCGATGATCCAGTGGTGGT‑‑ACGGCCACAACGCCGTGGGCTTCTTCCTCACCACCGGCTTCCTGGGga                                                            <  1:248677/150‑1 (MQ=255)
                                                                                            gctgcACATCGTCAACCACATCTCGCTGCCGGTGAGCCTGTTCAAGTCGTACTCGGCCTACTCCGGCGCCACCGACGCGATGATCCAGTGGTGGT‑‑ACGGCCACAACGCCGTGTGCTTCTTCCTCACCACCGGCTTCCTGGGGATGATGta                                                     <  2:1042829/150‑1 (MQ=255)
                                                                                            gctgcACATCGTCAACCACATCTCGCTGCCGGTGAGCCTGTTCAAGTCGTACTCGGCCTACTCCGGCGCCACCGACGCGATGATCCAGTGGTGGT‑‑ACGGCCACAACGCCGTGGGCTTCTTCCTCACCACCGGCTTCCTGGGGATGATGta                                                     <  1:382842/150‑1 (MQ=255)
                                                                                            gctgcACATCGTCAACCACATCTCGCTGCCGGTGAGCCTGTTCAAGTCGTACTCGGCCTACTCCGGCGCCACCGACGCGATGATCCAGTGGTGGT‑‑ACGGCCACAACGCCGTGGGCTTCTTCCTCACCACCGGCTTCCTGGGGATGATGta                                                     >  2:479378/1‑150 (MQ=255)
                                                                                               gcACATCGTCAACCACATCTCGCTGCCGGTGAGCCTGTTCAAGTCGTACTCGGCCTACTCCGGCGCCACCGACGCGATGATCCAGTGGTGGT‑‑ACGGCCACAACGCCGTGGGCTTCTTCCTCACCACCGGCTTCCTGGGGCTGATGtacta                                                  >  2:99752/1‑150 (MQ=255)
                                                                                                  caTCGTCAACCACATCTCGCTGCCGGTGAGCCTGTTCAAGTCGTACTCGGCCTACTCCGGCGCCACCGACGCGATGATCCAGTGGTGGT‑‑ACGGCCACAACGCCGTGGGCTTCTTCCTCACCACCGGCTTCCTGGGGATGATGTACTACtt                                               >  1:95623/1‑150 (MQ=255)
                                                                                                             acaTCTCGCTGCCGGTGAGCCTGTTCAAGTCGTACTCGGCCTACTCCGGCGCCACCGACGCGATGATCCAGTGGTGGT‑‑ACGGCCACAACGCCGTGGGCTTCTTCCTCACCACCGGCTTCCTGGGGATGATGTACTACTTCGTGCCCAAGc                                    >  2:234845/1‑150 (MQ=255)
                                                                                                              caTCTCGCTGCCGGTGAGCCTGTTCAAGTCGTACTCGGCCTACTCCGGTGCCACCGACGCGATGATCCAgtggtg                                                                                                                >  2:264333/1‑75 (MQ=255)
                                                                                                              caTCTCGCTGCCGGTGAGCCTGTTCAAGTCGTACTCGGCCTACTCCGGTGCCACCGACGCGATGATCCAgtggtg                                                                                                                <  1:264333/75‑1 (MQ=255)
                                                                                                              caTCTCGCTGCCGGTGAGCCTGTTCAAGTAGTACTCGGCCGACGCGCGGGCCGCGTACGCGCTGATCCATTTGTCGTTGGCGTCCACTTCGACTTGGG‑‑TCTTCCTCACCACCGGCTTCCTGGGGATGATGTGCAACTCCGGTCCCAAGCa                                   >  1:1177832/1‑150 (MQ=255)
                                                                                                                    gCTGCCGGTGAGCCTGTTCAAGTCGTACTCGTCCTACTCCGGCGCCACCGACGCGATGATCCAGTGGTGGT‑‑ACGGCCACAACGCCGTGGGCTTCTTCCTCCCCACCGGCTTCCTGGGGATGATGTACTACGTCGTGCCCAAGCAGGCCGa                             <  2:617305/150‑1 (MQ=255)
                                                                                                                     cTGCCGGTGAGCCTGTTCAAGTCGTACTCGGCCTACTCCGGCGCCACCGACGCGATGATCCAGTGGTGGT‑‑ACGGCCACAACGCCGTGGGCTTCTTCCTCACCACCGGCATGCTGAGGATGATGTAATACTTCGTGCCCAAGCAGGCCGAg                            >  1:62981/1‑150 (MQ=255)
                                                                                                                         cGGTGAGCCTGTTCAAGTCGTACTCGGCCTACTCCGGCGCCACCGACGCGATGATCCAGTGGTGGT‑‑ACGGCCACAACGCCGTGGGCTTCTTCCTCACCACCGGCTTCCTGGGGATGATGTACTACTTCGTGCCCAAGCAGGCCGAGCGGc                        <  2:826132/150‑1 (MQ=255)
                                                                                                                             gAGCCTGTTCAAGTCGTACTCGTCCTACTCCGGCGCCACCGACGCGATGATCCAGTGGTGGT‑‑ACGGCCACAACGCCGTGGGCTTCTTCCTCACCACCGGCTTCCTGGGGATGATGTACTACTTCGTGCCCAAGCAGGCCGAGCGGCCGAt                    <  1:466258/150‑1 (MQ=255)
                                                                                                                             gAGCCTGTTCAAGTCGTACTCGGCCTACTCCGGCGCCACCGACGCGATGATCCAGTGGTGGT‑‑ACGGCCACAACGCCGTGGGCTTCTTCCTCACCACCGGCTTCCTGGGGATGATGTACTACTTCGTGCCCAAGCAGGCCGAGCGGCCGAt                    <  2:95623/150‑1 (MQ=255)
                                                                                                                                ccTGTTCAAGTCGTACTCGGCCTACTCCGGCGCCACCGACGCGATGATCCAGTGGTGGT‑‑ACGGCCACAACGCCGTGGGCTTCTTCCTCACCACCGGCTTCCTGGGGATGATGTACTACTTCGTGCCCAAGCAGGCCGAGCGGCCGATCTa                 >  1:1115985/1‑150 (MQ=255)
                                                                                                                                  tGTTCAAGTCGTACTCGGCCTACTCCGGCGCCACCGACGCGATGATCCAGTGGTGGT‑‑ACGGCCACAACGCCGTgg                                                                                          >  1:329298/1‑75 (MQ=255)
                                                                                                                                  tGTTCAAGTCGTACTCGGCCTACTCCGGCGCCACCGACGCGATGATCCAGTGGTGGT‑‑ACGGCCACAACGCCGTgg                                                                                          <  2:329298/75‑1 (MQ=255)
                                                                                                                                  tGTTCAAGTCGTACTCGGCCTACTCCGGCGCCACCGACGCGATGATCCAGTGGTGGT‑‑ACGGCCACAACGCCGTGGGCTTCTTCCTCACCACCg                                                                        >  2:86674/1‑93 (MQ=255)
                                                                                                                                  tGTTCAAGTCGTACTCGGCCTACTCCGGCGCCACCGACGCGATGATCCAGTGGTGGT‑‑ACGGCCACAACGCCGTGGGCTTCTTCCTCACCACCg                                                                        <  1:86674/93‑1 (MQ=255)
                                                                                                                                         gTCGTACTCGGCCTACTCCGGCGCCACCGACGCGATGATCCAGTGGTGGT‑‑ACGGCCACAACGCCGTGGGCTTCTTCCTCACCACCGGCTTCCTGGGGATGATGTACTACTTCGTGCCCAAGCAGGCCGAGCGGCCGATCTACTCGTATCg        >  2:573091/1‑150 (MQ=255)
                                                                                                                                         gTCGTACTCGGCCTACTCCGGCGCCACCGACGCGATGATCCAGTGGTGGT‑‑ACGGCCACAACGCCGTGGGCTTCTTCCTCACCACCGGCTTCCTGGGGATGATGTACTACTTCGTGCCCAAGCAGGCCGAGCGGCCGATCTACTCGTATCg        >  2:957702/1‑150 (MQ=255)
                                                                                                                                              aCTCGGCCTACTCCGGCGCCACCGACGCGATGATCCAGTGGTGGT‑‑ACGGCCACAACGCCGTGGGCTTCTTCCTCACCACCGGCTTCCTGGGGATGATGTACTACTTCGTGCCCAAGCAGGCCGAGCGGCCGATCTACTCGTATCGCTTGt   >  1:693299/1‑150 (MQ=255)
                                                                                                                                               cTCGGCCTACTCCGGCGCCACCGACGCGATGATCCAGTGGTGGT‑‑ACGGCCACAACGCCGTGGGCTTCTTCCTCACCACCGGCTTCCTGGGGATGATGTACTACTTCGTGCCCAAGCAGGCCGAGCGGCCGATCTACTCGTATCGCTTGTc  <  1:860850/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
CTGGTGTTCTTCGGCACTATCGTCAAACGCAAGACCAAGCACATCTATGTCGGCAACTGGTTCTACGGTGCGTTCATCGTGGTCACCGCGATGCTGCACATCGTCAACCACATCTCGCTGCCGGTGAGCCTGTTCAAGTCGTACTCGGCCTACTCCGGCGCCACCGACGCGATGATCCAGTGGTGGT‑‑ACGGCCACAACGCCGTGGGCTTCTTCCTCACCACCGGCTTCCTGGGGATGATGTACTACTTCGTGCCCAAGCAGGCCGAGCGGCCGATCTACTCGTATCGCTTGTC  >  NC_002947/4840109‑4840401

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑