| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 4,850,203 | C→T | 14.4% | intergenic (+75/‑112) | hemN → / → fnrA | oxygen‑independent coproporphyrinogen III dehydrogenase/DNA‑binding transcriptional dual regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 4,850,203 | 0 | C | T | 14.4% | 129.7 / 13.1 | 56 | intergenic (+75/‑112) | hemN/fnrA | oxygen‑independent coproporphyrinogen III dehydrogenase/DNA‑binding transcriptional dual regulator |
| Reads supporting (aligned to +/- strand): ref base C (26/21); new base T (3/5); total (29/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.55e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.46e-01 | |||||||||||
GCGCGCTCGGTATGCATGGTGTTCGACGCGTATCTGGCGATGCACAACCGCCAGCGTTTCTCGCGAGTGATCTGAAAAACAAGGCGTTCAACCGCATGGACAAGCTTCCTTGTCAGGCACACTATGAGCACACAAGGACTCCCGGCAGGCCACCTGCCGGGGTTTCGCCAGCGCGCACCATCATCGCGCACACTGGCCTACACCCTGTGTCGTGAGTTACCCTTACGACTTATGTGTGCTTTCCCACAAGGATCGATTCAAATGTCCGAGCCAGTCAAACTGCGCCCACACAACCA > NC_002947/4850054‑4850349 | gcgcgcTCGGTATGCATGGTGTTCGACGCGTATCTGGCGATGCACAACCGCCAGCGTTTCTCGCGAGTGATCTGAAAAACAAGGCGTTCAACCGCATGGACAAGCTTCCTTGTCAGGCACACTATGAGCACACAAGGACTCCCGGCAGGc < 2:333918/150‑1 (MQ=255) gcgcTCGGTATGCATGGTGTTCGACGCGTATCTGGCGATGCACAACCGCCAGCGTTTCTCGCGAGTGATCTGAAAAACAAGGCGTTCAACCGCATGGACAAGCTTCCTTGTCAGGCACACTATGAGCACACAAGGACTCCCGGCAGGCCa > 2:671291/1‑150 (MQ=255) gcTCGGTATGCATGGTGTTCGACGCGTATCTGGCGATGCACAACCGCCAGCGTTTCTCGCGAGTGATCTGAAAAACAAGGCGTTCAACCGCATGGACAAGCTTCCTTGTCAGGCACACTATGAGCACACAAGGACTCCCGGCAGGCCAcc > 1:534460/1‑150 (MQ=255) cTCGGTATGCATGGTGTTCGACGCGTATCTGGCGATGCACAACCGCCAGCGTTTCTCGCGAGTGATCTGAAAAACAAGGCGTTCAACCGCATGGACAAGCTTCCTTGTCAGGCACACTATGAGCACACAAGGACTCCCGGCAGGCCACCt > 2:805087/1‑150 (MQ=255) tGCATGGTGTTCGACGCGTATCTGGCGATGCACAACCGCCAGCGTTTCTCGCGAGTGATCTGAAAAACAAGGCGTTCAACCGCATGGACAAGCTTCCTTGTCAGGCACACTATGAGCACACAAGGACTCCCGGCAGGTGGCCTGCCggga < 1:1158751/150‑2 (MQ=255) tGCATGGTGTTCGACGCGTATCTGGCGATGCACAACCGCCAGCGTTTCTCGCGAGTGATCTGAAAAACAAGGCGTTCAACCGCATGGACAAGCTTCCTTGTCAGGCACACTATGAGCACACAAGGACTCCCGGCAGGTGGCCTGCCggga < 1:816195/150‑2 (MQ=255) tGCATGGTGTTCGACGCGTATCTGGCGATGCACAACCGCCAGCGTTTCTCGCGAGTGATCTGAAAAACAAGGCGTTCAACCGCATGGACAAGCTTCCTTGTCAGGCACACTATGAGCACACAAGGACTCCCGGCAGGTGGCCTGCCggga < 2:580444/150‑2 (MQ=255) gACGCGTATCTGGCGATGCACAACCGCCAGCGTTTCTCGCGAGTGATCTGAAAAACAAGGCGTTCAACCGCATGGACAAGCTTCCTTGTCAGGCACACTATGAGCACACAAGGACTCCCGGCAGGCCACCTGCCGGGGTTTCGCCAgcgc > 1:898028/1‑150 (MQ=255) aCGCGTATCTGGCGATGCACAACCGCCAGCGTTTCTCGCGAGTGATCTGAAAAACAAGGCGTTCAACCGCATGGACAAGCTTCCTTGTCAGGCACACTATGAGCACACAAGGACTCCCGGCAGGCCACCTGCCGGGGTTTCGCCAgtgtg > 1:472717/1‑146 (MQ=255) tCTGGCGATGCACAACCGCCAGCGTTTCTCGCGAGTGATCTGAAAAACAAGGCGTTCAACCGCATGGACAAGCTTCCTTGTCAGGCACACTATGAGCACACAAGGACTCCCGGCAGGTGGCCTGCCggga > 1:399344/1‑129 (MQ=255) tCTGGCGATGCACAACCGCCAGCGTTTCTCGCGAGTGATCTGAAAAACAAGGCGTTCAACCGCATGGACAAGCTTCCTTGTCAGGCACACTATGAGCACACAAGGACTCCCGGCAGGTGGCCTGCCggga < 2:399344/130‑2 (MQ=255) caACCGCCAGCGTTTCTCGCGAGTGATCTGAAAAACAAGGCGTTCAACCGCATGGACAAGCTTCCTTGTCAGGCACACTATGAGCACACAAGGACTCCCGGCAGGCCACCTGCCGGGGTTTCGCCAGCGCGCACCATCATCGCGCACACt > 1:87456/1‑150 (MQ=255) aaCCGCCAGCGTTTCTCGCGAGTGATCTGAAAAACAAGGCGTTCAACCGCATGGACAAGCTTCCTTGTCAGGCACACTATGAGCACACAAGGACTCCCGGCAGGCCACCTGCCGGGGTTTCGCCAGCGCGCACCATCATCGCGCACACTg > 1:856421/1‑150 (MQ=255) aCCGCCAGCGTTTCTCGCGAGTGATCTGAAAAACAAGGCGTTCAACCGCATGGACAAGCTTCCTTGTCAGGCACACTATGAGCACACAAGGACTCCCGGCAGGCCACCTGCCGGGGTTTCGCCAGCGCGCACCATCATCGCGCACACTgg < 1:671291/150‑1 (MQ=255) gCCAGCGTTTCTCGCGAGTGATCTGAAAAACAAGGCGTTCAACCGCATGGACAAGCTTCCTTGTCAGGCACACTATGAGCACACAAGGACTCCCGGCAGGCCACCTGCCGGGGTTTCGCCAGCGCGCACCATCATCGCGCACACTGGCCt < 2:534460/150‑1 (MQ=255) cAGCGTTTCTCGCGAGTGATCTGAAAAACAAGGCGTTCAACCGCATGGACAAGCTTCCTTGTCAGGCACACTATGAGCACACAAGGACTCCCGGCAGGCCACCTGCCGGGGTTTCGCCAGCGCGCACCATCATCGCGCACACTGGCCTac < 1:39791/150‑1 (MQ=255) cgcgcgAGTGATCTGAAAAACAAGCCCTTCAACCGCATGGACAAGCTTCCTTGTCATGCACACTATGAGAACACAAGGCCTCCAGGCAGGCCACCTGCCGGGGTTGCGCCAGCGCGCACCATCACCGTGAACACTGGCCTACACCCtgtg < 1:527865/148‑1 (MQ=255) tcGCGAGTGATCTGAAAAACAAGGCGTTCAACCGCATGGACAAGCTTCCTTGTCAGGCACACTATGAGCACACAAGGACTCCCGGCAGGcc < 1:3784/91‑1 (MQ=255) tcGCGAGTGATCTGAAAAACAAGGCGTTCAACCGCATGGACAAGCTTCCTTGTCAGGCACACTATGAGCACACAAGGACTCCCGGCAGGcc > 2:3784/1‑91 (MQ=255) tcGCGAGTGATCTGAAAAACAAGGCGTTCAACCGCATGGACAAGCTTCCTTGTCAGGCACACTATGAGCACACAAGGACTCCCGGCAGGCCACCTGCCGGGGTTTCGCCAGCGCGCACCATCATCGCGCACACTGGCCTACACCCTgtgt > 2:1196705/1‑150 (MQ=255) cgcgAGTGATCTGAAAAACAAGGCGTTCAACCGCATGGACAAGCTTCCTTGTCAGGCACACTATGATCACACAAGGACTCCCGGCAGGTGGCCTGCCggga > 2:446805/1‑100 (MQ=255) cgcgAGTGATCTGAAAAACAAGGCGTTCAACCGCATGGACAAGCTTCCTTGTCAGGCACACTATGATCACACAAGGACTCCCGGCAGGTGGCCTGCCggga < 1:446805/101‑2 (MQ=255) aGTGATCTGAAAAACAAGGCGTTCAACCGCATGGACAAGCTTCCTTGTCAGGCACACTATGAGCACACAAGGACTCCCGGCAGGcc < 2:36608/86‑1 (MQ=255) aGTGATCTGAAAAACAAGGCGTTCAACCGCATGGACAAGCTTCCTTGTCAGGCACACTATGAGCACACAAGGACTCCCGGCAGGcc > 1:36608/1‑86 (MQ=255) aGTGATCTGAAAAACAAGGCGTTCAACCGCATGGACAAGCTTCCTTGTCAGGCACACTATGAGCACACAAGGACTCCCGGCAGGCCACCTGCCGGGGTTTCGCCAGCGCGCACCATCATCGCGCACACTGGCCTACACCCTGTGTCGTGa < 2:856421/150‑1 (MQ=255) tGATCTGAAAAACAAGGCGTTCAACCGCATGGACAAGCTTCCTTGTCAGGCACACTATGAGCACACAAGGACTCCCGGCAGGCCACCTGCCGGGGTTTCGCCAGCGCGCACCATCATCGCGCACACTGGCCTACACCCTGTGTCGTGAGt < 1:1196705/150‑1 (MQ=255) tGAAAAACAAGGCGTTCAACCGCATGGACAAGCTTCCTTGTCAGGCACACTATGAGCACACAAGGACTCCCGGCAGGCCACCTGCCGGGGTTTCGCCAGCGCGCACCATCATCGCGca > 2:400278/1‑118 (MQ=255) tGAAAAACAAGGCGTTCAACCGCATGGACAAGCTTCCTTGTCAGGCACACTATGAGCACACAAGGACTCCCGGCAGGCCACCTGCCGGGGTTTCGCCAGCGCGCACCATCATCGCGca < 1:400278/118‑1 (MQ=255) gAAAAACAAGGCGTTCAACCGCATGGACAAGCTTCCTTGTCAGGCACACTATGAGCACACAAGGACTCCCGGCAGGCCACCTGCCGGGGTTTCGCCAGCGCGCACCATCATCGCGCACACTGGCCTACACCCTGTGTCGTGAGTTACCCt > 1:671188/1‑150 (MQ=255) aaGGCGTTCAACCGCATGGACAAGCTTCCTTGTCAGGCACACTATGAGCACACAAGGACTCCCGGCAGGCCACCTGCCGGGGTTTCGCCAGCGCGCACCATCATCGCGCACACTGGCCTACACCCTGTGTCGTGAGTTACCCTTACGACt > 2:711741/1‑150 (MQ=255) ggCGTTCAACCGCATGGACAAGCTTCCTTGTCAGGCACACTATGAGCACACAAGGACTCCCGGCAGGCCACCTGCCGGGGTTTCGCCAGCGCGCACCATCATCGCGCACACTGGCCTACACCCTGTGTCGTGAGTTACCCTTACGACTTa < 1:805087/150‑1 (MQ=255) ttCAACCGCATGGACAAGCTTCCTTGTCAGGCACACTATGAGCACACAAGGACTCCCGGCAGGCCACCTGCCGGGGTTTCGCCAGCGCGCACCATCATCGCGCACACTGGCCTACACCCTGTGTCGTGAGTTACCCTTACGACTTAtgtg > 1:289220/1‑150 (MQ=255) cATGGACAAGCTTCCTTGTCAGGCACACTATGAGCACACAAGGACTCCCGGCAGGCCACCTGCCGGGGTTTCGCCAGCGCGCACCATCATCGCGCACACTGGCCTa < 2:720464/106‑1 (MQ=255) cATGGACAAGCTTCCTTGTCAGGCACACTATGAGCACACAAGGACTCCCGGCAGGCCACCTGCCGGGGTTTCGCCAGCGCGCACCATCATCGCGCACACTGGCCTa > 1:720464/1‑106 (MQ=255) aCAAGCTTCCTTGTCAGGCACACTATGAGCACACAAGGACTCCCGGCAGGCCACCTGCCGGGGTTTCGCCAGCGCGCACCATCATCGCGCACACTGGCCTACACCCTGTGTCGTGAGTTACCCTTACGCCTTATGTGTGCTTTCCCACaa < 2:898028/150‑1 (MQ=255) cAAGCTTCCTTGTCAGGCACACTATGAGCACACAAGGACTCCCGGCAGGCCACCTGCCGGGGTTTCGCCAGCGCGCACCATCATCGCGCACACTGGCCTACACCCTGTGTCGTGAGTTACCCTTACGACTTATGTGTGCTTTCCCACAAg < 2:87456/150‑1 (MQ=255) aaGCTTCCTTGTCAGGCACACTATGAGCACACAAGGACTCCCGGCAGGCCACCTGCCGGGGTTTCGCCAGCGCGCACCATCATCGCGCACACTGGCCTACACCCTGTGTCGTGAGTTACCCTTACGACTTATGTGTGCTTTCCCACAAgg > 1:841916/1‑150 (MQ=255) cTTGTCAGGCACACTATGAGCACACAAGGACTCCCGGCAGGCCACCTGCCGGGGTTTCGCCAGCGCGCACCATCATCGCGCACACTGGCCTACACCCTGTGTCGTGAGTTACCCTTACGACTTATGGCGGCGTTCCCACAAGGATCGAtt > 2:280308/1‑150 (MQ=255) tCAGGCACACTATGAGCACACAAGGACTCCCGGCAGGCCACCTGCCGGGGTTTCGCCAgcgc > 2:152740/1‑62 (MQ=255) tCAGGCACACTATGAGCACACAAGGACTCCCGGCAGGCCACCTGCCGGGGTTTCGCCAgcgc < 1:152740/62‑1 (MQ=255) cAGGCACACTATGAGCACACAAGGACTCCCGGCAGGCCACCTGCCGGGGTTTCGCCAGCGCGCACCATCATCGCGCACACTGGCCTACACCCTGTGTCGTGAGTTACCCTTACGACTTATGTGTGCTTTCCCACAAGGATCGATTCAAAt < 1:621999/150‑1 (MQ=255) cAGGCACACTATGAGCACACAAGGACTCCCGGCAGGCCACCTGCCGGGGTTTCGCCAGCGCGCACCATCATCGCGCACACTCGCCTACACCCTTTGTCGTGAGTTACCCTTACGACTTATGTGTGCTTTCCCACAAGGATCGATTCAAAt < 1:333155/150‑1 (MQ=255) tATGAGCACACAAGGACTCCCGGCAGGCCACCTGCCGGGGTTTCGCAAGCGCGCACCATCATCGCGCACACTGGCCTACACCCTGTGTCGTGAGTTACCCTTACGACTTATGTGTGCTTTCCCACAAGGATCGATTCAAATGTCCGAGcc > 2:462145/1‑150 (MQ=255) aTGAGCACACAAGGACTCCCGGCAGGCCACCTGCCGGGGTTTCGCCAGCGCGCACCATCATCGCGCACACTGGCCTACACCCTGTGTCGTGAGTTACCCTTACGACTTATGTGTGCTTTCCCACAAGGAt < 2:60426/130‑1 (MQ=255) aTGAGCACACAAGGACTCCCGGCAGGCCACCTGCCGGGGTTTCGCCAGCGCGCACCATCATCGCGCACACTGGCCTACACCCTGTGTCGTGAGTTACCCTTACGACTTATGTGTGCTTTCCCACAAGGAt > 1:60426/1‑130 (MQ=255) aTGAGCACACAAGGACTCCCGGCAGGCCACCTGCCGGGGGTTCGCCAGCGCGCACCATCATCGCGCACACTGGCCTACACCCTGTGTCGTGAGTTAACCTTACGACTTATGTGTGCTTTCCCACAAGGATCGATTAGAATGTCCGAGCCa > 1:1154450/1‑150 (MQ=255) ccaAGGTATCCCGACAGGACCACTCTCGCGGTTTCCCCAGCGCGCACCATCTGCTAGCACAGCGGCCTACACCCTGTTACGTGAGTAACCCCTACAACGTATGAGTGGTTTCCCACTAGCCTTGTTTCAAATGTCCGAGCCAGTCAAACt < 2:175194/149‑1 (MQ=255) caAGGACTCCCGGCCGGCCACCTGCCGGGGTTTCGCCAGCGCGCACCATCATCGCGCACACTGGCCTACACCCTGTGTCGTGAGTTACGCTTACGACTTATGTGTGCTTTCCCACAAAGATCGATTCAAATGTCCGCGCCAGTCAAACTg > 2:81435/1‑150 (MQ=255) aaGGACTCCCGGCAGGCCACCTGCCGGGGTTTCGCCAGCGCGCACCATCATCGCGCACACTGGCCTACACCCTGTGTCGTGAGTTACCCTTACGACTTATGTGTGCTTTCCCACAAGGATCGATTCAAATGTCCGAGCCAGTCAAACTgc > 2:724473/1‑150 (MQ=255) gaaaccccGGCAGGTGGCCTGCCGGGGTTTCGCCAGCGCGCACCATCATCGCGCACACTGGCCTACACCCTGTGTCGTGAGTTACCCTTACGACTTATGTGTGCTTTCCCACAAGGATCGATTCAAATGTCCGAGCCAGTCAAACTgcgc > 2:184724/6‑150 (MQ=255) tCCCGGCAGGCCACCTGCCGGGGTTTCGCCAGCGCGCACCATCATCGCGCACACTGGCCTACACCCTGTGTCGTGAGTTACCCTTACGACTTATGTGTGCTTTCCCACAAGGATCGATTCAAATGTCCGCGCCAATCAAGATGCGCCcac > 2:974909/1‑150 (MQ=255) ccGGCAGGCCACCTGCCGGGGTTTCGCCAGCGCGCACCATCATCGCGCACACTGGCCTACACCCTGTGTCGTGAGTTACCCTTACGACTTATGTGTGCTTTCCCACAAGGATCGATTCAAATGTCCGAGCCAGTCAAACTGCGCCcacac > 1:212000/1‑150 (MQ=255) cGGCAGGCCACCTGCCGGGGTTTCGCCAGCGCGCACCATCATCGCGCACACTGGCCTACACCCTGTGTCGTGAGTTACCCTTACGACTTATGTGTGCTTTCCCACAAGGATCGAt < 2:441019/115‑1 (MQ=255) cGGCAGGCCACCTGCCGGGGTTTCGCCAGCGCGCACCATCATCGCGCACACTGGCCTACACCCTGTGTCGTGAGTTACCCTTACGACTTATGTGTGCTTTCCCACAAGGATCGAt > 1:441019/1‑115 (MQ=255) cGGCAGGCCACCTGCCGGGGTTTCGCCAGCGCGCACCATCATCGCGCACACTGGCCTACACCCTGTGTCGTGAGTTACCCTTACGACTTATGTGTGCTTTCCCACAAGGATCGATTCAAATGTcc > 2:1106393/1‑125 (MQ=255) cGGCAGGCCACCTGCCGGGGTTTCGCCAGCGCGCACCATCATCGCGCACACTGGCCTACACCCTGTGTCGTGAGTTACCCTTACGACTTATGTGTGCTTTCCCACAAGGATCGATTCAAATGTcc < 1:1106393/125‑1 (MQ=255) ggCAGGCCACCTGCCGGGGTTTCGCCAGCGCGCACCATCATCGCGCACACTGGCCTACACCCTGTGTCGTGAGTTACCCTTACGACTTATGTGTGCTTTcc > 2:376966/1‑101 (MQ=255) ggCAGGCCACCTGCCGGGGTTTCGCCAGCGCGCACCATCATCGCGCACACTGGCCTACACCCTGTGTCGTGAGTTACCCTTACGACTTATGTGTGCTTTcc < 1:376966/101‑1 (MQ=255) gCAGGCCACCTGCCGGGGTTTCGCCAGCGCGCACCATCATCGCGCACACTGGCCTACACCCTGTGTCGTGAGTTACCCTTACGACTTATGTGTGCTTTCCCACAAGGATCGATTCAAATGTCCGAGCCAGTCAAACTGCGCCCACACAAc < 2:212000/150‑1 (MQ=255) aGGCCACCTGCCGGGGTTTCGCCAGCGCGCACCATCATCGCGCACACTGGCCTACACCCTGTGTCGTGAGTTACCCTTACGACTTATGTGTGCTTTCCCACAAGGATCGATTCAAATGTCCGAGCCAGTCAAACTGCGCCCACACAACCa < 1:724473/150‑1 (MQ=255) aGGCCACCTGCCGGGGTTTCGCCAGCGCGCACCATCATCGCGCACACTGGCCTACACCCTGTGTCGTGAGTTACCCTTACGACTTATGTGTGCTTTCCCACAAGGATCGATTCAAATGTCCGAGCCAGTCAAACTGCGCCCACACAACCa < 1:711741/150‑1 (MQ=255) | GCGCGCTCGGTATGCATGGTGTTCGACGCGTATCTGGCGATGCACAACCGCCAGCGTTTCTCGCGAGTGATCTGAAAAACAAGGCGTTCAACCGCATGGACAAGCTTCCTTGTCAGGCACACTATGAGCACACAAGGACTCCCGGCAGGCCACCTGCCGGGGTTTCGCCAGCGCGCACCATCATCGCGCACACTGGCCTACACCCTGTGTCGTGAGTTACCCTTACGACTTATGTGTGCTTTCCCACAAGGATCGATTCAAATGTCCGAGCCAGTCAAACTGCGCCCACACAACCA > NC_002947/4850054‑4850349 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |