Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,894,262 T→C 8.7% V162A (GTG→GCG)  PP_4302 → urea transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,894,2620TC 8.7% 209.3 / 6.8 78V162A (GTG→GCG) PP_4302urea transporter
Reads supporting (aligned to +/- strand):  ref base T (34/37);  new base C (3/4);  total (37/41)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01

ACGCACCAGTGGCGCAAGCGTGGCGGCAAGTTGCTGATTGCCTACACCTCCCCTTTCGTATTGCTGGGCTGGGCCACACTGCTGGTCGCCAGCCCCTCGCCCAGCGGGTTCGTCGAAGCCGACCCGCTGTATGCGTTGGCCCGCGGCGTGGGCCAGATTTTCCTGCTCGACCAGCCCTTGGCCGGGTTGCTGATCATCGTCGGCATGTTCATCGTCAATCCCTATGCGGCGATGTGGGCGGTGATCGGCTCGGCCATTGGTGGCGGCGTGGCGTTGCTCACCGATCAGGC  >  NC_002947/4894114‑4894403
                                                                                                                                                    |                                                                                                                                             
aCGCACCAGTGGCGCAAGCGTGGCGGCAAGTTGCTGATTGCCTACACCTCCCCTTTCGTATTGCTGGGCTGGGCCACACTGCTGGTCGCCAGCCCCTCGCCCAGCGGGTTCGTCGAAGCCGACCCGCTGTATGCGTTGGCCCGCGGCGTg                                                                                                                                              >  2:267736/1‑150 (MQ=255)
aCGCACCAGTGGCGCAAGCGTGGCGGCAAGTTGCTGATTGCCTACACCTCCCCTTTCGTATTGCTGGGCTGGGCCACACTGCTGGTCGCCAGCCCCTCGCCCAGCGGGTTCGTCGAAGCCGACCCGCTGTATGCGTTGGCCCGCGGCGTg                                                                                                                                              >  2:612505/1‑150 (MQ=255)
  gCACCAGTGGCGCAAGCGTGGCGGCAAGTTGCTGATTGCCGACACCTCCCCTTTCGTATTGCTGGGCTGGACCACACTGCTGGTCGCCAGCCCCTCGCCCAGCGGGTTCGTCGCAGCCGACCCGCTGTATGCGTTGGGCCTCGGCGTggg                                                                                                                                            >  1:1150156/1‑150 (MQ=255)
      cAGTGGCGCAAGCGTGGCGGCAAGTTGCTGATTGCCTACACCTCCCCTTTCGTATTGCTGGGCTGGGCCACACTGCTGGTCGCCAGCCCCTCGCCCAGCGGGTTCGTCGAAGCCGACCCGCTGTATGCGTTGGCCCACGCCGCGGGCCaa                                                                                                                                        <  2:913364/150‑2 (MQ=255)
       attGGCGCAAGCGTGGCGGCAAGTTGCTGATTGCCTACACCTCCCCTTTCGTATTGCTGGGCTGGGCCACACTGCTGGTCGCCAGCCCCTCGCCCAGCGGGTTCGTCGAAGCCGACCCGCTGTATGCGTTGGCCCACGCCGCGGGCCaac                                                                                                                                       <  2:433739/148‑3 (MQ=255)
            cgcAAGCGTGGCGGCAAGTTGCTGATTGCCTACACCTCCCCTTTCGTATTGCTGGGCTGGGCCACACTGCTGGTCGCCAGCCCCTCGCCCAGCGGGTTCGTCGAAGCCGACCCGCTGTATGCGTTGGCCCGCGGCGTGGGCCAGATTTTc                                                                                                                                  <  2:436586/150‑1 (MQ=255)
            cgcAAGCGTGGCGGCAAGTTGCTGATTGCCTACACCTCCCCTTTCGTATTGCTGGGCTGGGCCACACTGCTGGTCGCCAGCCCCTCGCCCAGCGGGTTCGTCGAAGCCGACCCGCTGTATGCGTTGGCCCGCGGCGTGGGCCAGATTTTc                                                                                                                                  <  1:859207/150‑1 (MQ=255)
                     ggcggcAAGTTGCTGATTGCCTACACCTCCCCTTTCGTATTGCTGGGCTGGGCCACACTGCTGGTCGCCAGCCCCTCGCCCAGCGGGTTCGTCGAAGCCGACCCGCTGTATGCGTTGGCCCGCGGCGTGGGCCAGATTTTCCTGCTCGAc                                                                                                                         <  2:930982/150‑1 (MQ=255)
                       cggcAAGTTGCTGATTGTCTACACCCCCCCTTTCGTATTGCTGGGCTGGGCCACACTGCTGGTCGCCAGCCCCTCGCCCAGCGGGTTCGTCGAAGCCGACCCGCTGTATGCGTTGGCCCGCGGCGTGGGCCAGATTTTCCTGCTCGACCa                                                                                                                       <  1:205546/150‑1 (MQ=255)
                       cggcAAGTTGCTGATTGCCTACACCTCCCCTTTCGTATTGCTGGGCTGGGCCACACTGCTGGTCGCCAGCCCCTCGCCCAGCGGGTTCGTCGAAGCCGACCCGCTGTATGCGTTGGCCCGCGGCGTGGGCCAGATTTTCCTGCTCGACCa                                                                                                                       >  1:828914/1‑150 (MQ=255)
                                    aTTGCCTACACCTCCCCTTTCGTATTGCTGGGCTGGGCCACACTGCTGGTCGCCAGCCCCTCGCCCAGCGGGTTCGTCGAAGCCGACCCGCTGTATGCGTTGGCCCGCGGCGTGGGCCAGATTTTCCTGCTCGACCAGCCCTTGGCCggg                                                                                                          <  1:972614/150‑1 (MQ=255)
                                    aTTGCCTACACCTCCCCTTTCGTATTGCTGGGCTGGGCCACACTGCTGGTCGCCAGCCCCTCGCCCAGCGGGTTCGTCGAAGCCGACCCGCTGTATGCGTTGGCCCACGCCGCGGGCCaac                                                                                                                                       >  1:693773/1‑119 (MQ=255)
                                    aTTGCCTACACCTCCCCTTTCGTATTGCTGGGCTGGGCCACACTGCTGGTCGCCAGCCCCTCGCCCAGCGGGTTCGTCGAAGCCGACCCGCTGTATGCGTTGGCCCACGCCGCGGGCCaac                                                                                                                                       <  2:693773/121‑3 (MQ=255)
                                     ttGCCTACACCTCCCCTTTCGTATTGCTGGGCTGGGCCACACTGCTGGTCGCCAGCCCCTCGCCCAGCGGGTTCGTCGAAGCCGACCCGCTGTATGCGTTGGCCCGCGGCGTGGGCCAGATTTTCCTGCTCGACCAGCCCTTGGCCGGGt                                                                                                         >  1:1115182/1‑150 (MQ=255)
                                        ccTACACCTCCCCTTTCGTATTGCTGGGCTGGGCCACACTGCTGGTCGTCACCCCCTCGCCCAGCGGGTTCGTCGAAGCCGACCCGCTGTATGCGTTGGCCCGCGGCGTGGGCCAGATTTTCCTGCTCGACCAGCCCTTGGCCGGGTTGc                                                                                                      >  1:937076/1‑150 (MQ=255)
                                        ccTACACCTCCCCTTTCGTATTGCTGGGCTGGGCCACACTGCTGGTCGCCAGCCCCTCGCCCAGCGGGTTCGTCGAAGCCGACCCGCTGTATGCGTTGGCCCGCGGCGTGGGCCAGATTTTCCTGCTCGACCAGCCCTTGGCCGGGTTGc                                                                                                      <  1:1010778/150‑1 (MQ=255)
                                        ccTACACCTCCCCTTTCGTATTGCTGGGCTGGGCCACACTGCTGGTCGCCAGCCCCTCGCCCAGCGGGTTCGTCGAAGCCGACCCGCTGTATGCGTTGGCCCGCGGCGTGGGCCAGATTTTCCTGCTCGACCAGCCCTTGGCCGGGTTGc                                                                                                      >  2:1041524/1‑150 (MQ=255)
                                        ccTACACCTCCCCTTTCGTATTGCTGGGCTGGGCCACACTGCTGGTCGCCAGCCCCTCGCCCAGCGGGTTCGTCGAAGCCGACCCGCTGTATGCGTTGGCCCGCGGCGTGGGCCAGATTTTCCTGCTCGACCAGCCCTTGGCCGGGTTGc                                                                                                      >  1:206194/1‑150 (MQ=255)
                                        ccTACACCTCCCCTTTCGTATTGCTGGGCTGGGCCACACTGCTGGTCGCCAGCCCCTCGCCCAGCGGGTTCGTCGAAGCCGACCCGCTGTATGCGTTGGCCCGCGGCGTGGGCCAGATTTTCCTGCTCGACCAGCCCTTGGCCGGGTTGc                                                                                                      <  1:267736/150‑1 (MQ=255)
                                         cTACACCTCCCCTTTCGTATTGCTGGGCTGGGCCACACTGCTGGTCGCCAGCCCCTCGCCCAGCGGGTTCGTCGAAGCCGACCCGCTGTATGCGTTGGCCCGCGGCGTGGGCCAGATTTTc                                                                                                                                  >  1:523367/1‑121 (MQ=255)
                                         cTACACCTCCCCTTTCGTATTGCTGGGCTGGGCCACACTGCTGGTCGCCAGCCCCTCGCCCAGCGGGTTCGTCGAAGCCGACCCGCTGTATGCGTTGGCCCGCGGCGTGGGCCAGATTTTc                                                                                                                                  <  2:523367/121‑1 (MQ=255)
                                          tACACCTCCCCTTTCGTATTGCTGGGCTGGGCCACACTGCTGGTCGCCAGCCCCTCGCCCAGCGGGTTCGTCGAAGCCGACCCGCTGTATGCGTTGGCCCGCGGCGTGGGCCAGa                                                                                                                                       <  2:737633/115‑1 (MQ=255)
                                          tACACCTCCCCTTTCGTATTGCTGGGCTGGGCCACACTGCTGGTCGCCAGCCCCTCGCCCAGCGGGTTCGTCGAAGCCGACCCGCTGTATGCGTTGGCCCGCGGCGTGGGCCAGa                                                                                                                                       >  1:737633/1‑115 (MQ=255)
                                             ccccccccTTTCGTATTGCTGGGCTGGGCCACACTCCTGGTCGCCAGCCCCTCGCCCAGCGGGTTCGTCGAAGCCGACCCGCTGTATGCGTTGGCCCGCGGCGTGGGCCAGATTTTCCTGCTCGACCAGCCCTTGGCCGGGTTGCTGatc                                                                                                 <  2:571290/146‑1 (MQ=255)
                                                tCCCCTTTCGTATTGCTGGGCTGGGCCACACTGCTGGTCGCCAGCCCCTCGCCCAGCGGGTTCGTCGAAGCCGACCCGCTGTATGCGTTGGCCCGCGGCGTGGGCCAGATTTTCCTGCTCGACCAGccc                                                                                                                   >  1:39418/1‑129 (MQ=255)
                                                tCCCCTTTCGTATTGCTGGGCTGGGCCACACTGCTGGTCGCCAGCCCCTCGCCCAGCGGGTTCGTCGAAGCCGACCCGCTGTATGCGTTGGCCCGCGGCGTGGGCCAGATTTTCCTGCTCGACCAGccc                                                                                                                   <  2:39418/129‑1 (MQ=255)
                                                tCCCCTTTCGTATTGCTGGGCTGGGCCACACTGCTGGTCGCCAGCCCCTCGCCCAGCGGGTTCGTCGAAGCCGACCCGCTGTATGCGTTGGCCCACGCCGCGGGCCaa                                                                                                                                        <  2:85523/108‑2 (MQ=255)
                                                tCCCCTTTCGTATTGCTGGGCTGGGCCACACTGCTGGGCGCCAGCCCCTCGCCCAGCGGGTTCGTCGAAGCCTACCCGCTGTATGCGTTGGCCCACGCCGCGGGCCaa                                                                                                                                        >  1:85523/1‑107 (MQ=255)
                                                   ccTTTCGTATTGCTGGGCTGGGCCACACTGCTGGTCGCCAGCCCCTCGCCCAGCGGGTTCGTCGAAGCCGACCCGCTGTATGCGTTGGCCCGCGGCGTGGGCCAGATTTTCCTGCTCGACCAGCCCTTGGCCGGGTTGCTGATCAtcgtc                                                                                           <  2:694193/150‑1 (MQ=255)
                                                          tATTGCTGGGCTGGGCCACACTGCTGGTCGCCAGCCCCTCGCCCAGCGGGTTCGTCGAAGCCGACCCGCTGTATGCGTTGGCCCGCGGCGTGGGCCAGATTTTCCTGCTCGACCAGCCCTTGGCCGGGTTGCTGATCATCGTCGGCATGt                                                                                    <  2:1115182/150‑1 (MQ=255)
                                                          tATTGCTGGGCTGGGCCACACTGCTGGTCGCCAGCCCCTCGCCCAGCGGGTTCGTCGAAGCAGACCCGCTGTATGCGTTGGCACGCGGCGCGGGCGAGATGTTCCTGCTCGACCAGCCCTTGGCCGGGGTGCTGATCATCGTCGGCATGt                                                                                    >  1:573699/1‑150 (MQ=255)
                                                           aTTGCTGGGCTGGGCCACACTGCTGGTCGCCAGCCCCTCGCCCAGCGGGTTCGTCGAAGCCGACCCGCTGTATGCGTTGGCCCGCGGCGTGGGCCAGATTTTCCTGCTCGACCAGCCCTTGGCCGGGTTGCTGATCATCGTCGGCATGtt                                                                                   >  1:522071/1‑150 (MQ=255)
                                                            ttGCTGGGCTGGGCCACACTGCTGGTCGCCAGCCCCTCGCCCAGCGGGTTCGTCGAAGCCGACCCGCTGTATGCGTTGGCCCGCGGCGTGGGCCAGATTTTCCTGCTCGACCAGCCCTTGGCCGGGTTGCTGATCATCGTCGGCATGTTc                                                                                  >  1:412750/1‑150 (MQ=255)
                                                                tgggctgggcCACACTGCTGGTCGCCAGCCCCTCGCCCAGCGGGTTCGTCGAAGCCGACCCGCTGTATGCGTTGGCCCGCGGCGTGGGCCAGAtt                                                                                                                                     >  2:1158546/1‑95 (MQ=255)
                                                                tgggctgggcCACACTGCTGGTCGCCAGCCCCTCGCCCAGCGGGTTCGTCGAAGCCGACCCGCTGTATGCGTTGGCCCGCGGCGTGGGCCAGAtt                                                                                                                                     <  1:1158546/95‑1 (MQ=255)
                                                                tgggctgggcCACACTGCTGGTCGCCAGCCCCGCGCCCAGCGGGTTCGTCGAAGCCGACCCGCTGTATGCGTTGGCCCGCGGCGTGGGCCAGATTTTCCTGCTCGACCAGCCCTTGGCCGGGTTGCTGATCATCGTCGGCATGTTCATCg                                                                              <  1:508740/150‑1 (MQ=255)
                                                                  ggctgggcCACACTGCTGGTCGCCAGCCCCTCGCCCAGCGGGTTCGTCGAAGCCGACCCGCTGTATGCGTTGGCCCGCGGCGTGGGCCAGATTTTCCTGCTCGACCAGCCCTTGGCCGGGTTGCTGATCATCGTCGGCATGTTCATCGtg                                                                            <  2:828914/150‑2 (MQ=255)
                                                                         ccACACTGCTGGTCGCCAGCCCCTCGCCCAGCGGGTTCGTCGAAGCCGACCCGCTGTATGCGTTGGCCCGCGGCGTGGGCCAGATTTTCCTGCTCGACCAGCCCTTGGCCGGGTTGCTGATCATCGTCGGCATGTTCATCGTCAATCCCt                                                                     >  2:207698/1‑150 (MQ=255)
                                                                         ccACACTGCTGGTCGCCAGCCCCTCGCCCAGCGGGTTCGTCGAAGCCGACCCGCTGTATGCGTTGGCCCGCGGCGTGGGCCAGATTTTCCTGCTCGACCAGCCCTTGGCCGGGTTGCTGATCATCGTCGGCATGTTCATCGTCAATCCCt                                                                     >  2:710043/1‑150 (MQ=255)
                                                                           acacTGCTGGTCGCCAGCCCCTCGCCCAGCGGGTTCGTCGAAGCCGACCCGCTGTATGCGTTGGCCCGCGGCGTGGGCCAGATTTTCCTGCTCGATCAGCCCTTGGCCGGGTTGCTGATCATCGTCGGCATGTTCATCGTCAATCCCTAt                                                                   <  2:206194/150‑1 (MQ=255)
                                                                             acTGCTGGTCGCCAGCCCCTCGCCCAGCGGGTTCGTCGAAGCCGACCCGCTGTATGCGTTGGCCCGCGGCGTGGGCCAGATTTTCCTGCTCGACCAGCCCTTGGCCGGGTTGc                                                                                                      >  1:719421/1‑113 (MQ=255)
                                                                             acTGCTGGTCGCCAGCCCCTCGCCCAGCGGGTTCGTCGAAGCCGACCCGCTGTATGCGTTGGCCCGCGGCGTGGGCCAGATTTTCCTGCTCGACCAGCCCTTGGCCGGGTTGc                                                                                                      <  2:719421/113‑1 (MQ=255)
                                                                             acTGCTGGTCGCCAGCCCCTCGCCCAGCGGGTTCGTCGAAGCCGACCCGCTGTATGCGTTGGCCCGCGGCGTGGGCCAGATTTTCCTGCTCGACCAGCCCTTGGCCGGGTTGCTGATCATCGTCGGCATGTTCATCGTCAATCCCTATgc                                                                 <  1:207698/150‑1 (MQ=255)
                                                                             acTGCTGGTCGCCAGCCCCTCGCCAAGCGGGTTCGTCGAAGCCGACCCGCTGTATGCGTTGGCCCGCGGCGTGGGCCAGATTTTCCTGCTCGACCAGCCCTTGGCCGGGTTGCTGa                                                                                                   >  2:602714/1‑116 (MQ=255)
                                                                             acTGCTGGTCGCCAGCCCCTCGCCAAGCGGGTTCGTCGAAGCCGACCCGCTGTATGCGTTGGCCCGCGGCGTGGGCCAGATTTTCCTGCTCGACCAGCCCTTGGCCGGGTTGCTGa                                                                                                   <  1:602714/116‑1 (MQ=255)
                                                                                  tgGTCGCCAGCCCCTCGCCCAGCGGGTTCGTCGAAGCCGACCCGCTGTATGCGTTGGCCCGCGGCGTGGGCCAGATTTTCCTGCTCGACCAGCCCtt                                                                                                                 >  2:1128692/1‑97 (MQ=255)
                                                                                  tgGTCGCCAGCCCCTCGCCCAGCGGGTTCGTCGAAGCCGACCCGCTGTATGCGTTGGCCCGCGGCGTGGGCCAGATTTTCCTGCTCGACCAGCCCtt                                                                                                                 <  1:1128692/97‑1 (MQ=255)
                                                                                  tgGTCGCCAGCCCCTCGCCCAGCGGGTTCGTCGAAGCCGACCCGCTGTATGCGTTGGCCCGCGGCGTGGGCCAGATTTTCCTGCTCGACCAGCCCTTGGCCGGGTTGCTGATCATCGTCGGCATGTTCATCGTCAATCCCTATGCGGCGa                                                            >  2:466768/1‑150 (MQ=255)
                                                                                     tCGCCAGCCCCTCGCCCAGCGGGTTCGTCGAAGCCGACCCGCTGTATGCGTTGGCCCGCGGCGTGGGCCAGATTTTCCTGCTCGACCAGCCCTTGGCCGGGTTGCTGATCATCGTCGGCATGTTCATCGTCAATCCCTATGCGGCGAtgt                                                         <  2:115759/150‑1 (MQ=255)
                                                                                        ccAGCCCCTCGCCCAGCGGGTTCGTCGAAGCCGACCCGCTGTATGCGTTGGCCCGCGGCGTGGGCCAGATTTTCCTGCTCGACCAGCCCTTGGCCGGGTTGCTGATCATCGTCGGCATGTTCATCGTCAATCCCTATGCGGCGATGTggg                                                      >  1:396585/1‑150 (MQ=255)
                                                                                        ccAGCCCCTCGCCCAGCGGGTTCGTCGAAGCCGACCCGCTGTATGCGTTGGCCCGCGGCGTGGGCCAGATTTTCCTGCTCGACCAGCCCTTGGCCGGGTTGCTGATCATCGTCGGCATGTTCATCGTCAATCCCTATGCGGCGATGTggg                                                      <  1:1041524/150‑1 (MQ=255)
                                                                                         cAGCCCCTCGCCCAGCGGGTTCGTCGAAGCCGACCCGCTGTATGCGTTGGCCCGCGGCGTGGGCCAGATTTTCCTGCTCGACCAGCCCTTGGCCGGGTTGCTGATCATCGTCGGCATGTTCATCGTCAATCCCTATGCGGCGTTGTGGGc                                                     >  1:987513/1‑150 (MQ=255)
                                                                                              ccTCGCCCAGCGGGTTCGTCGAAGCCGACCCGCTGTATGCGTTGGCCCGCGGCGTGGGCCAGATTTTCCTGCTCGACCAGCCCTTGGCCGGGTTGCTGATCATCGTCGGCATGTTCATCGTCAATCCCTATGCGGCGATGTGGGCGGTGa                                                >  1:1017355/1‑150 (MQ=255)
                                                                                               cTCGCCCAGCGGGTTCGTCGAAGCCGACCCGCTGTATGCGTTGGCCCGCGGCGTGGGCCAGATTTTCCTGCTCGACCAGCCCTTGGCCGGGTTGCTGATCATCGTCGGCATGTTCATCGTCAATCCCTATGCGGCGATGTGGGCGGTGAt                                               <  2:1017355/150‑1 (MQ=255)
                                                                                                  gCCCAGCGGGTTCGTCGAAGCCGACCCGCTGTATGCGTTGGCCCGCGGCGTGGGCCAGATTTTCCTGCTCGACCAGCCCTTGGCCGGGTTGCTGATCATCGTCGGCATGTTCATCGTCAATCCCTATGCGGCGATGTGGGCGGTGAtcgg                                            <  2:522071/150‑1 (MQ=255)
                                                                                                     cAGCGGGTTCGTCGAAGCCGACCCGCTGTATGCGTTGGCCCGCGGCGTGGGCCAGATTTTCCTGCTCGACCAGCCCTTGGCCGGGTTGCTGATCATCGTCGGCATGTTCATCGTCAATCCCTATGCGGCGATGTggg                                                      <  2:1105415/137‑1 (MQ=255)
                                                                                                     cAGCGGGTTCGTCGAAGCCGACCCGCTGTATGCGTTGGCCCGCGGCGTGGGCCAGATTTTCCTGCTCGACCAGCCCTTGGCCGGGTTGCTGATCATCGTCGGCATGTTCATCGTCAATCCCTATGCGGCGATGTggg                                                      >  1:1105415/1‑137 (MQ=255)
                                                                                                           gTTCGTCGAAGCCGACCCGCTGTATGCGTTGGCCCGCGGCGTGGGCCAGATTTTCCTGCTCGACCAGCCCTTGGCCGGGTTGCTGATCATCGTCGGCATGTTCATCGTCAATCCCTATGCGGCGATGTGGGCGGTGATCGGCTCGGCCAt                                   <  2:835068/150‑1 (MQ=255)
                                                                                                           gTTCGTCGAAGCCGACCCGCTGTAGGCGTTGGCCCGCGGCGTGGGCCAGATTTTCCTGCTCGACCAGCCCTTGGCCGGGTTGCTGATCATCGTCGGCATGTTCATCGTCAATCCCTATGCGGCGATGTGGGCGGTGATCGGCTCGGCCAt                                   >  2:677348/1‑150 (MQ=255)
                                                                                                            ttCGTCGAAGCCGACCCGCTGTATGCGTTGGCCCGCGGCGTGGGCCAGATTTTCCTGCTCGACCAGCCCTTGGCCGGGTTGCTGATCATCGTCGGCATGTTCATCGTCAATCCCTATGCGGCGATGTGGGCGGTGATCGGCTCGGCCAtt                                  <  1:466768/150‑1 (MQ=255)
                                                                                                            ttCGTCGAAGCCGACCCGCTGTATGCGTTGGCCCGCGGCGTGGGCCAGATTTTCCTGCTCGACCAGCCCTTGGCCGGGTTGCTGATCATCGTCGGCATGTTCATCGTCAATCCCTATGCGGCGATGTGGGCGGTGATCGGCTCGGCCAtt                                  <  1:1085669/150‑1 (MQ=255)
                                                                                                               gtcgAAGCCGACCCGCTGTATGCGTTGGCCCGCGGCGTGGGCCAGATTTTCCTGCTCGACCAGCCCTTGGCCGGGTTGCTGATCATCGTCGGCATGTTCATCGTCAATCCCTATGCGGCGATGTGGGCGGTGATCGGCTCGGCCATtggt                               >  1:778491/1‑150 (MQ=255)
                                                                                                               gtcgAAGCCGACCCGCTGTATGCGTTGGCCCGCGGCGTGGGCCAGATTTTCCTGCTCGACCAGCCCTTGGCCGGGTTGCTGATCATCGTCGGCATGTTCATCGTCAATCCCTATGCGGCGATGTGGGCGGTGATCGGCTCGGCCATtggt                               >  2:280958/1‑150 (MQ=255)
                                                                                                                  gAAGCCGACCCGCTGTATGCGTTGGCCCGCGGCGTGGGCCAGATTTTCCTGCTCGACCAGCCCTTGGCCGGGTTGCTTATCATCGTCGGCATGTTCATCGTCAATCCCTATGCGGCGATGTGGGCGGTGATCGGCTCGGCCATTGGTggc                            <  2:700886/150‑1 (MQ=255)
                                                                                                                  gAAGCCGACCCGCTGTATGCGTTGGCCCGCGGCGTGGGCCAGATTTTCCTGCTCGACCAGCCCTTGGCCGGGTTGCTGATCATCGTCGGCATGTTCATCGTCAATCCCTATGCGGCGATGTGGGCGGTGATCGGCTCGGCCATTGGTggc                            >  2:587404/1‑150 (MQ=255)
                                                                                                                        gACCCGCTGTATGCGTTGGCCCGCGGCGTGGGCCAGATTTTCCTGCTCGACCAGCCCTTGGCCGGGTTGCTGATCATCGTCGGCATGTTCATCGTCAATCCCTATGCGGCGATGTGGGCGGTGATCGGCTCGGCCATTGGTGGCggcgtg                      >  2:851357/1‑150 (MQ=255)
                                                                                                                         aCCCGCTGTATGCGTTGGCCCGCGGCGTGGGCCAGATTTTCCTGCTCGACCAGCCCTTGGCCGGGTTGCTGATCATCGTCGGCATGTTCATCGTCAATCCCTATGCGGCGATGTGGGCGGTGATCGGCTCGGCCATTGGTGGCggcgtgg                     <  1:851357/150‑1 (MQ=255)
                                                                                                                         aCCCGCTGTATGCGTTGGCCCGCGGCGTGGGCCAGATTTTCCTGCTCGACCAGCCCTTGGCCGGGTTGCTGATCATCGTCGGCATGTTCATCGTCAATCCCTATGCGGCGATGTGGGCGGTGATCGGCTCGGCCATTGGTGGCggcgtgg                     >  1:1047026/1‑150 (MQ=255)
                                                                                                                            cGCTGTATGCGTTGGCCCGCGGCGTGGGCCAGATTTTCCTGCTCGACCAGCCCTTGGCCGGGTTGCTGATCATCGTCGGCATGTTCATCGTCAATCCCTATGCGGCGATGTGGGCGGTGATCGGCTCGGCCATTGGTGGCggcgtggcgt                  >  1:1041196/1‑150 (MQ=255)
                                                                                                                               tGTATGCGTGGGCCCGCGGCGTGGGCCAGATTTTCCTGCTCGACCAGCCCTTGGCCGGGTTGCTGATCATCGTCGGCATGTTCATCGTCAATCCCTATGCGGCGATGTGGGCGGTGATCGGCTCGGCCATTGGTGGCGGCGTGGCGTTGc               <  2:573699/150‑1 (MQ=255)
                                                                                                                                  aTGCGTTGGCCCGCGGCGTGGGCCAGATTTTCCTGCTCGACCAGCCCTTGGCCGGGTTGCTGATCATCGTCGGCATGTTCATCGTCAATCCCTATGCGGCGATATGGGCGGTGATCGGCTCGGCCATTGGTGGCGGCGTGGCGTTGCTCa            <  2:1150156/150‑1 (MQ=255)
                                                                                                                                      gTTGGCCCGCGGCGTGGGCCAGATTTTCCTGCTCGa                                                                                                                          >  1:864674/1‑36 (MQ=255)
                                                                                                                                      gTTGGCCCGCGGCGTGGGCCAGATTTTCCTGCTCGa                                                                                                                          <  2:864674/36‑1 (MQ=255)
                                                                                                                                         ggCCCGCGGCGTGGGCCAGATTTTCCTGCTCGACCAGCCCTTGGCCGGGTTGCTGATCATCGTCGGCATGTTCATCGTCAATCCCTATGCGGCGATGTGGGCGGTGATCGGCTCGGCCATTGGTGGCGGCGTGGCGTTGCTCACCGATCa     <  2:987513/150‑1 (MQ=255)
                                                                                                                                         ggCCCGCGGCGTGGGCCAGATTTTCCTGCTCGACCAGCCCTTGGCCGGGTTGCTGATCATCGTCGGCATGTTCATCGTCAATCCCTATGCGGCGATGTGGGCGGTGATCGGCTCGGCCATTGGTGGCGGCGTGGCGTTGCTCACCGATCa     >  1:376443/1‑150 (MQ=255)
                                                                                                                                          gCCCGCGGCGTGGAGTAGATTTTCCTGCTCGACCAGCCCTTGGCCGGGTTGCTGATCATCGTCGGCATGTTCATCGTCAATCCCTATGCGGCGATGTGGGCGGTGATCGGCTCGGCCATTGGTGGCGGCGTGGCGTTGCTCACCGATCAg    <  2:937076/150‑1 (MQ=255)
                                                                                                                                           cccGCGGCGTGGGCCAGATTTTCCTGCTCGACCAGCCCTTGGCCGGGTTGCTGATCATCGTCGGCATGTTCATCGTCAATCCCTATGCGGCGATGTGGGCGGTGATCGGCTCGGCCATTGGTGGCGGCGTGGCGTTGCTCACCGATCAgg   <  1:911285/150‑1 (MQ=255)
                                                                                                                                            ccGCGGCGTGGGCCAGATTTTCCTGCTCGACCAGCCCTTGGCCGGGTTGCTGATCATCGTCGGCATGTTCATCGTCAATCCCTATGCGGCGATGTGGGCGGTGATCGGCTCGGCCATTGGTGGCGGCGTGGCGTTGCTCACCGATCAGgc  >  1:161388/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                             
ACGCACCAGTGGCGCAAGCGTGGCGGCAAGTTGCTGATTGCCTACACCTCCCCTTTCGTATTGCTGGGCTGGGCCACACTGCTGGTCGCCAGCCCCTCGCCCAGCGGGTTCGTCGAAGCCGACCCGCTGTATGCGTTGGCCCGCGGCGTGGGCCAGATTTTCCTGCTCGACCAGCCCTTGGCCGGGTTGCTGATCATCGTCGGCATGTTCATCGTCAATCCCTATGCGGCGATGTGGGCGGTGATCGGCTCGGCCATTGGTGGCGGCGTGGCGTTGCTCACCGATCAGGC  >  NC_002947/4894114‑4894403

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑