| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 4,908,071 | G→C | 10.6% | intergenic (‑2/‑280) | PP_4317 ← / → PP_4318 | hypothetical protein/transposase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 4,908,071 | 0 | G | C | 10.6% | 128.4 / 7.5 | 47 | intergenic (‑2/‑280) | PP_4317/PP_4318 | hypothetical protein/transposase |
| Reads supporting (aligned to +/- strand): ref base G (22/20); new base C (2/3); total (24/23) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.66e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TGTTGTTGCTGTTGCTGTTGCCGTTGCCGTTGCCGTTGCCGTTGCCGTTGATCTTGATCTTGATCTTGATCTTGATCTTGATCTTGATCTTGATCTTCGCGACTTCAGGAGGCCGAACGCAGGCCTTGCGGAGGCAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCAGGATGGGGCCTTCGGCGCGGTCCTGCCGGGAGCGAGGCCGGAGTGAGGGGACCCCGGAGCGCAGCGCAGGGGCCGGATGATGGGAGCGCAGCGTTTTTTGGTTACTTTTTGTCGCGTTTGA > NC_002947/4907922‑4908217 | tgttgtTGCTGTTGCTGTTGCCGTTGCCGTTGCCGTTGCCGTTGCCGTTGATCTTGATCTTGATCTTGATCTTGATCTTGATCTTGATCTTGATCTTCGCGACTTCAGGAGGCCGAACGCAGGCCTTGCGGAGGCAGGTGACGGGCATgg > 2:706167/1‑150 (MQ=255) gttgctgttgctgttgcCGTTGCCGTTGCCGTTGCCGTTGCCGTTGATCTTGATCTTGATCTTGATCTTGATCTTGATCTTGATATTGATCTTCGCGCCTTCAGGAGGCCGAGCGCAGGCCTTGCGGAGGCAGGTGACGGGCATGGATGc > 2:512274/1‑150 (MQ=255) ctgttgctgttgcCGTTGCCGTTGCCGTTGCCGTTGCCGTTGATCTTGATCTTGATCGTGATCTTGATCTTGATCTTGATCTTGATCTTCGCGACTTCAAGAGGCCGAACGCAGGCCTTGCGGAGGCAGGTGACGGGCATCCATGCCCGt < 2:1010105/150‑1 (MQ=14) ctgttgcCGTTGCCGTTGCCGTTGCCGTTGCCGTTGATCTTGATCTTGATCTTGATCTTGATCTTGATCTTGATCTTGATCTTCGCGACTTCAGGAGGCCGAACGCAGGCCTTGCGGAGGCAGGTGACGGGCATGGATGCCCGTCAAgcg > 2:597793/1‑150 (MQ=39) ccgttgccgttgccgttgccgttgccgttgATCTTGATCTTGATCTTGATCTTGATCTTGATCTTGATCTTGATCTTCGCGACTTCAGGAGGCCGAACGCAGGCCTTGCGGAGGCAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAgg > 2:772232/1‑150 (MQ=39) ccgttgccgttgccgttgccgttgATCTTGATCTTGATCTTGATCTTGATCTTGATCTTGATCTTGATCTTCGCGACTTCAGGAGGCCGAACGCAGGCCTTGCGGAGGCAGGTGACGGGCATGGATGCCCGTCAAGCGCTGa > 1:224099/1‑142 (MQ=39) ccgttgccgttgccgttgccgttgATCTTGATCTTGATCTTGATCTTGATCTTGATCTTGATCTTGATCTTCGCGACTTCAGGAGGCCGAACGCAGGCCTTGCGGAGGCAGGTGACGGGCATGGATGCCCGTCAAGCGCTGa < 2:224099/142‑1 (MQ=38) gttgccgttgccgttgccgttgATCTTGATCTTGATCTTGATCTTGATCTTGATCTTGATCTTGATCTTCGCGACTTCAGGAGGCCGAACGCAGGCCTTGCGGAGGCAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCa < 1:805952/147‑1 (MQ=39) gttgccggtgccgttgccgttgATCTTGATCTTGATCTTGATCTTGATCTTGATCTTGATCTTGATCTTCGCGACTTCAGGAGGCCGAACGCAGGCCTTGCGGAGGCAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCa > 2:805952/1‑147 (MQ=38) ccgttgccgttgccgttgATCTTGATCTTGATCTTGATCTTGATCTTGATCTTGATCTTGATCTTCGCGACTTCAGGAGGCCGAACGCAGGCCTTGCGGAGGCAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCAGGATggg < 1:628954/150‑1 (MQ=39) gttgccgttgccgttgATCTTGATCTTGATCTTGATCTTGATCTTGATCTTGATCTTGATCTTCGCGACTTCAGGAGGCCGAACGCAGGCCTTGCGGAGGCAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCAGGATGGGGc < 1:597793/150‑1 (MQ=37) ttgccgttgccgttgATCTTGATCTTGATCTTGATCTTGATCTTGATCTTGATCTTGATCTTCGCGACTTCAGGAGGCCGAACGCAGGCCTTGCGGAGGCAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCAGGATGGGGcc > 1:738544/1‑150 (MQ=39) cgttgccgttgATCTTGATCTTGATCTTGATCTTGATCTTGATCTTGATCTTGATCTTCGCGACTTCAGGAGGCCGAACGCAGGCCTTGCGGAGGCAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCAGGATGGGGCCTTCg < 2:833296/150‑1 (MQ=35) gCCGTTGATCTTGATCTTGATCTTGATCTTGATCTTGATCTTGATCTTGATCTTCGCGACTTCAGGAGGCCGAACGCAGGCCTTGCGGAGGCAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCAGGATGGGGCCTTCGgcgc > 1:512274‑M2/1‑146 (MQ=255) tcttgatcttgatcttgatcttgatcttCGCGACTTCAGGAGGCCGAACGCAGGCCTTGCGGAGGCAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCACAGGATGGGGCCTTCGGCGCGGTCCTGCCGGGAGCGAGGCCGGAGt > 2:4635/1‑150 (MQ=17) ttgatcttgatcttgatcttgatcttCGCGACTTCAGGAGGCCGAACGCAGGCCTTGCGGAGGCAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCAGGATGGGGCCTTCGGCGCGGTCCTGCCGGGAGCGAGGCCGGAGTGa < 1:730043/150‑1 (MQ=32) ttgatcttgatcttgatcttCGCGACTTCAGGAGGCCGAACGCAGGCCTTGCGGAGGCCGGTGACGGGCATGGAGGCCCGTCAAGCGCTGAGGCCCCAGGATGGGGCCTTCGGCGCGGTCCTGCCGGGAGCGAGGCCGGAGTGAGGGGAc > 1:147174/1‑150 (MQ=17) ttgatcttgatcttCGCGACTTCAGGAGGCCGAACGCAGGCCTTGCGGAGGCAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCAGGATGGGGCCTTCGGCGCGGTCCTGCCGGGAGCGAGGCCGGAGTGAGGGGACCCCGGa < 2:1122160/150‑1 (MQ=32) ttgatcttgatcttCGCGACTTCAGGAGGCCGAACGCAGGCCTTGCGGAGGCAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCAGGACGGGGCCTTCGGCGCGGTCCTGCCGGGAGCGAGGCCGGAGTGAGGGGACCCCGGa > 1:519523/1‑150 (MQ=17) atcttgatcttCGCGACTTCAGGAGGCCGAACGCAGGCCTTGCGGAGGCAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCAGGATGGGGCCTTCGGCGCGGTCCTGCCGGGAGCGAGGCCGGAGTGAGGGGAcccctgcgct > 1:359465/1‑144 (MQ=17) ttgatcttCGCGACTTCAGGAGGCCGAACGCAGGCCTTGTGGAGGCAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCAGGATGGGGCCTTCGGCGCGGTCCTGCCGGGAGCGAGGCCGGAGTGAGGGGACCCCGGagcgcag > 2:832574/1‑150 (MQ=17) ttgatcttCGCGACTTCAGGAGGCCGAACGCAGGCCTTGCGGAGGCAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCAGGATGGGGCCTTCGGCGCGGTCCTGCCGGGAGCGAGGCCGGAGTGAGGGGACCCCGGagcgcag > 1:792924/1‑150 (MQ=32) gatcttCGCGACTTCAGGAGGCCGAACGCAGGCCTTGCGGAGGCAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCAGGATGGGGCCTTCGGCGCGGTCCTGCCGGGAGCGAGGCCGGAGTGAggg < 1:307109/133‑1 (MQ=33) gatcttCGCGACTTCAGGAGGCCGAACGCAGGCCTTGCGGAGGCAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCAGGATGGGGCCTTCGGCGCGGTCCTGCCGGGAGCGAGGCCGGAGTGAggg > 2:307109/1‑133 (MQ=33) ttCGCGACTTCAGGAGGCCGAACGCAGGCCTTGCGGAGGCAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCAGGATGGGGCCTTCGgc > 1:140495‑M2/1‑94 (MQ=255) ttCGCGACTTCAGGAGGCCGAACGCAGGCCTTGCGGAGGCAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCAGGATGGGGCCTTCGgc < 2:140495‑M2/96‑3 (MQ=255) cTTCAGGAGGCCGAACGCAGGCCTTGGGGCGGCGGGTGAGGGGGATGGATGCCCGTCAAGCGTTGAGGCCCCAGGATGGGGCCTTCGGCGCGGTCCTGCCGGGAGCGAGGCCGGAGTGAGGGGACCCCGGAGCGCAGCGCAGGGGCCGga < 2:864921/150‑1 (MQ=11) cTTCAGGAGGCCGAACGCAGGCCTTGCTTAGGCAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCAGGATGGGGCCTTCGGCGCGGTCCTGCCGGGAGCGAGGCCGGAGTGAGGGGACCCCGGAGCGCAGCGCAGGGGCCGga < 1:907183/150‑1 (MQ=17) ggaggCCGAACGCAGGCCTTGCGGAGGCAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCAGGATGGGGCCTTCGGCGCGGTCCTGCCGGGAGCGAGGCCGGAGTGAGGGGACCCCGGAGCGCAGCGCAGGGGCCGGatgatg < 1:706167/150‑1 (MQ=32) gaggCCGAACGCAGGCCTTGCGGAGGCAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCAGGATGGGGCCTTCGGCGCGGTCCTGCCGGGAGCGAGGCCGGAGTGAGGGGACCCCGGAGCGCAGCGCAGGGGCCGGATGATgg < 1:966539/150‑1 (MQ=32) gaggCCGAACGCAGGCCTTGCGGAGGCAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCACAGGATGGGGCCTTCGGCGCGGTCCTGCCGGGAGCGAGGCCGGAGTGAGGGGACCCCGGAGCGCAGCGCAGGGGCCGGATGATgg < 1:4635/150‑1 (MQ=17) gCCGAACGCAGGCCTTGCGGAGGCAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCAGGATGGGGCCTTCGGCGCGGTCCTGCCGGGAGCGAGGCCGGAGTGAGGGGACCCCGGAGCGCAGCGCAGGGGCCGGATGATGGGAg > 1:340507/1‑150 (MQ=32) cGCAGGCCTTGCGGAGGCAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCAGGATGGGGCCTTCGGCGCGGTCCTGCCGGGAGCGAGGCCGGAGTGAGGGGACCCCGGAGCGCAGCGCAGGGGCCGGATGATGGGAGCGCAGc < 2:307801/150‑1 (MQ=32) cTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAGGCGCTGAGGCCCCAGGATGGGGCCTTCGGCGCGGTCCTGCCGGGAGCGAGGCCGGAGTGAGGGGACCCCGGAGCGCAGCGCAGGGGCCGGATGATGGGAGCGCAGCGtttttt < 2:267877/150‑1 (MQ=17) ttGCGGAGGCAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCAGGATGGGGCCTTCGGCGCGGTCCTGCCGGGAGCGAGGCCGGAGTGAGGGGACCCCGGAGCGCAGCGCAGGGGCCGGATGATGGGAGCGCAGCGTTTTTTg > 2:169403/1‑150 (MQ=32) ttGCGGAGGCAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCAGGATGGGGCCTTCGGCGCGGTCCTGCCGGGAGCGAGGCCGGAGTGAGGGGACCCCGGAGCGCAGCGCAGGGGCCGGATGATGGGAGCGCAGCGTTTTTTg < 1:307765/150‑1 (MQ=32) tGCGGAGGCAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCAGGATGGGGCCTTCGGCGCGGTCCTGCCGGGAGCGAGGCCGGAGTGAGGGGACCCCGGAGCGCAGCGCAGGGGCCGGATGATGGGAGCGCAGCGTTTTTTgg < 2:738544/150‑1 (MQ=32) ggAGGCAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCAGGATGGGGCCTTCGGCGCGGTCCTGCCGGGAGCGAGGCCGGAGTGAGGGGACCCCGGAGCGCAGCGCAGGGGCCGGATGATGGGAGCGCAGCGTTTTTTGGTTa > 1:837499/1‑150 (MQ=32) aGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCAGGATGGGGCCTTTGGCGCGGTCCTGCCGGGAGCGAGGCCGGAGTGAGGGGACCCCGGAGCGCAGCGCAGGGGCCGGATGATGGGAGCGCAGCGTTTTTTGGTTACttttt < 1:865974/150‑1 (MQ=17) aGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCAGGATGGGGCCTTCGGCGCGGTCCTGCCGGGAGCGAGGCCGGAGTGAGGGGACCCCGGAGCGCAGCGCAGGGGCCGGATGATGGGAGCGCAGCGTTTTTTGGTTACttttt < 2:837499/150‑1 (MQ=32) aGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCAGGATGGGGCCTTCGGCGCGGTCCTGCCGGGAGCGAGGCCGGAGTGAGGGGACCCCGGAGCGCAGCGCAGGGGCCGGATGATGGGAGCGCAGCGTTTTTTGGTTACttttt > 2:392631/1‑150 (MQ=32) aGGTCACCGCCATGCACGCCCGTCAAGCGCTGAGTCCCCAGGATGGGGCCTTCGGCGCGGTCCTGCCGGGAGCGAGGCCGGAGTGAGGGGACCCCGGAGCGCAGCGCAGGGGCCGGATGATGGGAGCGCAGCGTTTTTTGGTTACttttt < 2:671720/150‑1 (MQ=11) gTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCAGGATGGGGCCTTCGGCGCGGTCCTGCCGGGAGCGAGGCCGGAGTGAGGGGACCCCGGAGCGCAGCGCAGGGGCCGGATGATGGGAGCGCAGCGTTTTTTGGTTACTTTTTGt > 1:948873/1‑150 (MQ=32) gACGGGCATCCATGCCCGTCAAGCGCTGAGGCCCCAGGATGGGGCCTTCGGCGCGGTCCTGCCGGGAGCGAGGCCGGAGTGAGGGGACCCCGGAGCGCAGCGCAGGGGCCGGATGATGGGAGCGCAGCGTTTTTTGGTTACttttt < 2:534921/146‑1 (MQ=12) gACGGGCATCCATGCCCGTCAAGCGCTGAGGCCCCAGGATGGGGCCTTCGGCGCGGTCCTGCCGGGAGCGAGGCCGGAGTGAGGGGACCCCGGAGCGCAGCGCAGGGGCCGGATGATGGGAGCGCAGCGTTTTTTGGTTACttttt > 1:534921/1‑146 (MQ=12) gACGGGCATCCATGCCCGTCAAGCGCTGAGGCCCCAGGATGGGGCCTTCGGCGCGGTCCTGCCGGGAGCGAGGCCGGAGTGAGGGGACCCCGGAGCGCAGCGCAGGGGCCGGATGATGGGAGCGCAGCGTTTTTTGGTTACTTTTTGTcg > 2:76443/1‑150 (MQ=12) aCGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCAGGCTGGGGCCTTATGCGCGGTCCTGCCGGTAGCGAGGCCGGAGTGAGGGGACCCCGGAGCGCAGCGCAGGGGCCGGATGATGGGAGCGCAGCGTTTTTTGGTTACTTTTTGTcgc > 1:329100/1‑150 (MQ=255) aTGGATGCCCGTCAAGCGCTGAGGCCCCAGGATGGGGCCTTCGGCGCGGTCCTGCCGGGAGCGAGGCCGGAGTGAGGGGACCCCGGAGCGCAGCGCAGGGGCCGGATGATGGGAGCGCAGCGTTTTTTGGTTACTTTTTGTCGCGTTTGa > 2:745807/1‑150 (MQ=32) | TGTTGTTGCTGTTGCTGTTGCCGTTGCCGTTGCCGTTGCCGTTGCCGTTGATCTTGATCTTGATCTTGATCTTGATCTTGATCTTGATCTTGATCTTCGCGACTTCAGGAGGCCGAACGCAGGCCTTGCGGAGGCAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCAGGATGGGGCCTTCGGCGCGGTCCTGCCGGGAGCGAGGCCGGAGTGAGGGGACCCCGGAGCGCAGCGCAGGGGCCGGATGATGGGAGCGCAGCGTTTTTTGGTTACTTTTTGTCGCGTTTGA > NC_002947/4907922‑4908217 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |