| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 5,030,799 | C→T | 21.1% | intergenic (+37/‑458) | PP_4433 → / → dadA‑I | amino acid MFS transporter/D‑amino acid dehydrogenase small subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 5,030,799 | 0 | C | T | 21.1% | 66.5 / 15.7 | 38 | intergenic (+37/‑458) | PP_4433/dadA‑I | amino acid MFS transporter/D‑amino acid dehydrogenase small subunit |
| Reads supporting (aligned to +/- strand): ref base C (15/15); new base T (3/5); total (18/20) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.97e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.07e-01 | |||||||||||
ACCTGCCTACTACATCATGGTTGCATCAGCACTTGGGATTATTGCGTTGTTCTTTGTGATCGAGACTAAAGGTTGCTCGATTCGCGGGGATGGAATACCTGGGGCAAAATAACCTAATAGGCAGATGGTATAGAGCCTGGGTGCTAATCAGCACCCAGGCTGTTGCGAAGCTCCATTCCTCCGGGCAAGCCGACCGGCTACGTCGATATAAGCAGCAGCGCTCCACCCTTCCTTTTTATTCTTATCTCACCTCTTGGCATCTGGCCCTGATAACAGTGTCTTCGCTATCTCTTG > NC_002947/5030651‑5030944 | aCCTGCCTACTACATCATGGTTGCATCAGCACTTGGGATTATTGCGTTGTTCTTTGTGATCGAGACTAAAGGTTGCTCGATTCGCGGGGATGGAATACCTGGGGCAAAATAACCTAATAGGCAGATGGTATAGAGCCTGGGTGCTAATCa > 1:1156149/1‑150 (MQ=255) gCCTACTACATCATGGTTGCATCAGCACTTGGGATTATTGCGTTGTTCTTTGTGATCGAGACTAAAGGTTGCTCGATTCGCGGGGATGGAATACCTGGGGCAAAATAACCTAATAGGCAGATGGTATAGAGCCTGGGTGCTGATTAGCAc > 1:308759/1‑150 (MQ=255) ccTACTACATCATGGTTGCATCAGCACTTGGGATTATTGCGTTGTTCTTTGTGATCGAGACTAAAGGTTGCTCGATTCGCGGGGATGGAATACCTGGGGCAAAATAACCTAATAGGCAGATGGTATAGAGCCTGGGTGCTGATTAGCAcc < 2:308759/150‑1 (MQ=255) tactacATCATGGTTGCATCAGCACTTGGGATTATTGCGTTGTTCTTTGTGATCGAGACTAAAGGTTGCTCGATTCGCGGGGATGGAATACCTGGGGCAAAATAACCTAATAGGCAGATGGTATAGAGCCTGGGTGCTGATTAGCACCCa < 2:147758/150‑1 (MQ=255) tactacATCATGGTTGCATCAGCACTTGGGATTATTGCGTTGTTCTTTGTGATCGAGACTAAAGGTTGCTCGATTCGCGGGGATGGAATACCTGGGGCAAAATAACCTAATAGGCAGATGGTATAGAGCCTGGGTGCTGATTAGCACCCa < 2:689022/150‑1 (MQ=255) actacATCATGGTTGCATCAGCACTTGGGATTATTGCGTTGTTCTTTGTGATCGAGACTAAAGGTTGCTCGATTCGCGGGGATGGAATACCTGGGGCAAAATAACCTAATAGGCAGATGGTATAGAGCCTGGGTGCTAATCAGCACCCAg < 1:118437/150‑1 (MQ=255) actacATCATGGTTGCATCAGCACTTGGGATTATTGCGTTGTTCTTTGTGATCGAGACTAAAGGTTGCTCGATTCGCGGGGATGGAATACCTGGGGCAAAATAACCTAATAGGCAGATGGTATAGAGCCTGGGTGCTAATCAGCACCCAg > 1:376287/1‑150 (MQ=255) tacATCATGGTTGCATCAGCACTTGGGATTATTGCGTTGTTCTTTGTGATCGAGACTAAAGGTTGCTCGATTCGCGGGGATGGAATACCTGGGGCAAAATAACCTAATAGGCAGATGGTATAGAGCCTGGGTGCTAATCAGCACCCAGGc > 2:947434/1‑150 (MQ=255) tacATCATGGTTGCATCAGCACTTGGGATTATTGCGTTGTTCTTTGTGATCGAGACTAAAGGTTGCTCGATTCGCGGGGATGGAATACCTGGGGCAAAATAACCTAATAGGCAGATGGTATAGAGCCTGGGTGCTAATCAGCACCCAGGc < 2:376287/150‑1 (MQ=255) ttGCATCAGCACTTGGGATTATTGCGTTGTTCTTTGTGATCGAGACTAAAGGTTGCTCGATTCGCGGGGATGGAATACCTGGGGCAAAATAACCTAATAGGCAGATGGTATAGAGCCTGGGTGCTAATCAGCACCCAGGCTGTTGCGAAg > 1:836280/1‑150 (MQ=255) gCATCAGCACTTGGGATTATTGCGTTGTTCTTTGTGATCGAGACTAAAGGTTGCTCGATTCGCGGGGATGGAATACCTGGGGCAAAATAACCTAATAGGCAGATGGTATAGAGCCTGGGTGCTAATCAGCACCCAGGCTGTTGCGAAGCt < 1:289771/150‑1 (MQ=255) cATCAGCACTTGGGATTATTGCGTTGTTCTTTGTGATCGAGACTAAAGGTTGCTCGATTCGCGGGGATGGAATACCTGGGGCAAAATAACCTAATAGGCAGATGGTATAGAGCCTGGGTGCTAATCAGCACCCAGGCTGTTGCGAAGCTc < 2:836280/150‑1 (MQ=255) tgttCTTTGTGATCGAGACTAAAGGTTGCTCGATTCGCGGGGATGGAATACCTGGGGCAAAATAACCTAATAGGCAGATGGTATAGAGCCTGGGTGCTGATTAGCACCCAgg < 1:459583/112‑1 (MQ=255) tgttCTTTGTGATCGAGACTAAAGGTTGCTCGATTCGCGGGGATGGAATACCTGGGGCAAAATAACCTAATAGGCAGATGGTATAGAGCCTGGGTGCTGATTAGCACCCAgg > 2:459583/1‑112 (MQ=255) ttGTGAACGTGACTTAACGTTGCTCGATTCGGTGGGATTGAATACCTGGGGCAAAATAACCTAATAGGCAGATGGTATAGAGCCTGGGTGCTAATCAGCACCCAGGCTGTTGCGAAGCTCCATTCCTCCGGGCAAGCCGACCGGCTACGt < 2:561136/150‑1 (MQ=255) tgATCGAGACTAAAGGTTGCTCGATTCGCGGGGATGGAATACCTGGGGCAAAATAACCTAATAGGCAGATGGTATAGAGCCTGGGTGCTAATCAGCACCCAGGCTGTTGCGAAGCTCCATTCCTCCGGGCAAGCCGACCGGCTACGTCGa > 1:596816/1‑150 (MQ=255) gagaCTAAAGGTTGCTCGATTCGCGGGGATGGAATACCTGGGGCAAAATAACCTAATAGGCAGATGGTATAGAGCCTGGGTGCTAATCAGCACCCAGGCTGTTGCGAAGCTCCATTCCTCCGGGCAAGCCGACCGGCTACGTCGATATaa < 2:1156149/150‑1 (MQ=255) cTAAAGGTTGCTCGATTCGCGGGGATGGAATACCTGGGGCAAAATAACCTAATAGGCAGATGGTATAGAGCCTGGGTGCTAATCAGCACCCAGGCTGTTGCGAAGCTCCATTCCTCCGGGCAAGCCGACCGGCTACGTCGATATAagcag > 1:941296/1‑150 (MQ=255) cTCGATTCGCGGGGATGGAATACCTGGGGCAAAATAACCTAATAGGCAGATGGTATAGAGCCTGGGTGCTAATCAGCACCCAGGCTGTTGCGAAGCTc > 1:758242/1‑98 (MQ=255) cTCGATTCGCGGGGATGGAATACCTGGGGCAAAATAACCTAATAGGCAGATGGTATAGAGCCTGGGTGCTAATCAGCACCCAGGCTGTTGCGAAGCTc < 2:758242/98‑1 (MQ=255) tCGATTCGCGGGGATGGAATACCTGGGGCAAAATAACCTAATAGGCAGATGGTATAGAGCCTGGGTGCTAATCAGCACCCAGGCTGTTGCGAAGCTCCATTCCTCCGGGCAAGCCGACCGGCTACGTCGATATAAGCAGCAGCGCTCCAc > 2:363119/1‑150 (MQ=255) tCGATTCGCGGGGATGGAATACCTGGGGCAAAATAACCTAATAGGCAGATGGTATAGAGCCTGGGTGCTAATCAGCACCCAGGCTGTTGCGAAGCTCCATTCCTCCGGGCAAGCCGACCGGCTACGTCGATATAAGCAGCAGCGCTCCAc > 1:970922/1‑150 (MQ=255) gATTCGCGGGGATGGAATACCTGGGGCAAAATAACCTAATAGGCAGATGGTATAGAGCCTGGGTGCTAATCAGCACCCAGGCTGTTGCGAAGCTCCATTCCTCCGGGCAAGc < 2:1115181/112‑1 (MQ=255) gATTCGCGGGGATGGAATACCTGGGGCAAAATAACCTAATAGGCAGATGGTATAGAGCCTGGGTGCTAATCAGCACCCAGGCTGTTGCGAAGCTCCATTCCTCCGGGCAAGc > 1:1115181/1‑112 (MQ=255) tGGGGCAAAATAACCTAATAGGCAGATGGTATAGAGCCTGGGTGCTGATTAGCACCCAgg > 2:292425/1‑60 (MQ=37) tGGGGCAAAATAACCTAATAGGCAGATGGTATAGAGCCTGGGTGCTGATTAGCACCCAgg < 1:292425/60‑1 (MQ=37) gCAAAATAACCTAATAGGCAAATGGTATAGAGCCTGGGTGCTAATCAGCACCCAGGCTGTTGCGAAGCTCCATTCCTCACGCCAAAACGACTGGCTAGGGCGATATTAGCAGCAGCGCTCCACCCTTCCTTTTTATTCTTATCTCACctc > 1:745167/1‑150 (MQ=255) aaaTAACCTAATAGGCAGATGGTATAGAGCCTGGGTGCTAATCAGCACCCAGGCTGTTGCGAAGCTCCATTCCTCCGGGCAAGCCGAcc < 1:816940/89‑1 (MQ=255) aaaTAACCTAATAGGCAGATGGTATAGAGCCTGGGTGCTAATCAGCACCCAGGCTGTTGCGAAGCTCCATTCCTCCGGGCAAGCCGAcc > 2:816940/1‑89 (MQ=255) gCAGATGGTATAGAGCCTGGGTGCTAATCAGCACCCAGGCTGTTGCGAAGCTCCATTCCTCCGGGCAAGCCGACCGGCTACGTCGATATAAGCAGCAGCGCTCCACCCTTCCTTTTTATTCTTATCTCACCTCTTGGCATCTGGCCCTGa < 1:947434/150‑1 (MQ=255) tGGTATCGAGCCTGGGTGCTAATCAGCACCCAGGCTGTTGCGAAGCTCCATTCCTCCGGGCAAGCAGTCTTGCGACGTCGATATAAGCAGCAGCGCTCCACCCTTCCTTTTTATTCTTATCTCACCTCTTGGCATCTGGCCCTGATAACa < 1:74693/150‑1 (MQ=255) tGGTATAGAGCCTGGGTGCTAATCAGCACCCAGGCTGTTGCGAAGCTCCATTCCTCCGGGCAAGCCGACCGGCTACGTCGATATAAGCAGCAGCGCTCCACCCTTCCTTTTTATTCTTATCTCACCTCTTGGCATCTGGCCCTGATAACa < 2:941296/150‑1 (MQ=255) agCCTGGGTGCTAATCAGCACCCAGGCTGTTGCGAAGCTCCATTCCTCCGGGCAAGCCGACCGGCTACGTCGATATAAGCAGCAGCGCTCCACCCTTCCTTTTTATTCTTATCTCACCTCTTGGCATCTGGCCCTGATAACAGTGTCTTc < 1:363119/150‑1 (MQ=255) ccTGGGTGCTAATCAGCACCCAGGCTGTTGCGAAGCTCCATTCCTCCGGGCAAGCCGACCGGCTACGTCGATATAAGCAGCAGCGCTCCACCCTTCCTTTTTATTCTTATCTCACCTCTTGGCATCTGGCCCTGATAACAgt < 2:1021772/142‑1 (MQ=255) ccTGGGTGCTAATCAGCACCCAGGCTGTTGCGAAGCTCCATTCCTCCGGGCAAGCCGACCGGCTACGTCGATATAAGCAGCAGCGCTCCACCCTTCCTTTTTATTCTTATCTCACCTCTTGGCATCTGGCCCTGATAACAgt > 1:1021772/1‑142 (MQ=255) cTGGGTGCTAATCAGCACCCAGGCTGTTGCGAAGCTCCATTCCTCCGGGCAAGCCGACCGGCTACGTCGATATAAGCAGCAGCGCTCCACCCTTCCTTTTTATTc > 2:1045301/1‑105 (MQ=255) cTGGGTGCTAATCAGCACCCAGGCTGTTGCGAAGCTCCATTCCTCCGGGCAAGCCGACCGGCTACGTCGATATAAGCAGCAGCGCTCCACCCTTCCTTTTTATTc < 1:1045301/105‑1 (MQ=255) tAATCAGCACCCAGGCTGTTGCGAAGCTCCATTCCTCCGGGCAAGCCGACCGGCTACGTCGATATAAGCAGCAGCGCTCCACCCTTCCTTTTTATTCTTATCTCACCTCTTGGCATCTGGCCCTGATAACAGTGTCTTCGCTATCTCTTg > 2:911405/1‑150 (MQ=255) | ACCTGCCTACTACATCATGGTTGCATCAGCACTTGGGATTATTGCGTTGTTCTTTGTGATCGAGACTAAAGGTTGCTCGATTCGCGGGGATGGAATACCTGGGGCAAAATAACCTAATAGGCAGATGGTATAGAGCCTGGGTGCTAATCAGCACCCAGGCTGTTGCGAAGCTCCATTCCTCCGGGCAAGCCGACCGGCTACGTCGATATAAGCAGCAGCGCTCCACCCTTCCTTTTTATTCTTATCTCACCTCTTGGCATCTGGCCCTGATAACAGTGTCTTCGCTATCTCTTG > NC_002947/5030651‑5030944 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |