Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,039,701 A→C 7.6% I284S (ATC→AGC) ‡ PP_4446 ← group II intron‑encoding maturase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,039,7010AC 7.6% 131.1 / 5.0 53I284S (ATC→AGC) ‡PP_4446group II intron‑encoding maturase
Reads supporting (aligned to +/- strand):  ref base A (27/22);  new base C (2/2);  total (29/24)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.29e-01

GATTACGCTGGCTACGACGTTTGAGGGCTTGCCGCCATAAACGGCAGACCGCCGAACGAAAACCACCCAGACGTGTCAGGTTTCCCGGCACCGCGTGGTAATTGAAGTAACCGCTCACCACCCGTGTGAGCCATTGCCCTTGAACCCAGATGGGTTCATGACGTCGGTGTTTCAGCTCATCCCGCATGGCCAGCAGCATCGCACGCATTCGCTTCTTGACCGTCAGTCGCAGTATTTGAAACCCACCGCTTCTGTTGGTACTACAACAGTGCGTGAAGCCCAGGAAGTCAAAC  >  NC_002947/5039552‑5039844
                                                                                                                                                     |                                                                                                                                               
gATTACGCTGGCTACGACGTTTGAGGGCTTGCCACCATAAACGGCAGACCGCCGAACGAAAACCACCCAGACGTGTCAGGTTTCCCGGCACCGCGTGGTAATTGAAGTAACCGCTCACCACCCGTGTGAGCCATTGCCCTTGAACCCAGa                                                                                                                                                 >  1:770198/1‑150 (MQ=255)
   tACGCTGGCTACGACGTTTGAGGGCTTGCCGCCATAAACGGCAGACCGCCGAACGAAAACCACCCAGACGTGTCAGGTTTCCCGGCACCGCGTGGTAATTGAAGTAACCGCTCACCACCCGTGTGAGCCATTGCCCTTGAACCCAGATgg                                                                                                                                              <  1:714247/150‑1 (MQ=255)
      gCTGGCTACGACGTTTGAGGGCTTGCCGCCATAAACGGCAGACCGCCGAACGAAAACCACCCAGACGTGTCAGGTTTCCCGGCACCGCGTGGTAATTGAAGTAACCGCTCACCACCCGTGTGAGCCATTGCCCTTGAACCCATCTGGGtt                                                                                                                                           >  2:762558/1‑150 (MQ=255)
        tGGCTACGACGTTTGAGGGCTTGCCGCCATAAACGGCAGACCGCCGAACGAAAACCACCCAGACGTGTCAGGTTTCCCGGCACCGCGTGGTAATTGAAGTAACCGCTCACCACCCGTGTGAGCCATTGCCCTTGAACCCAGATGGGTTCa                                                                                                                                         <  1:908714/150‑1 (MQ=255)
        tGGCTACGACGTTTGAGGGCTTGCCGCCATAAACGGCAGACCGCCGAACGAAAACCACCCAGACGTGTCAGGTTTCCCGGCACCGCGTGGTAATTGAAGTAACCGCTCACCACCCGTGTGAGCCATTGCCCTTGAACCCAGATGGGTTCa                                                                                                                                         <  1:846076/150‑1 (MQ=255)
         ggCTACGACGTTTGAGGGCTTGCCACCATAAACGGCAGACCGCCGAACGAAAACCACCCAGACGTGTCAGGTTTCCCGGCACCGCGTGGTAATTGAAGTAACCGCTCACCACCCGTGTGAGCCATTGCCCTTGAACCCAGATGGGTTCAt                                                                                                                                        <  2:770198/150‑1 (MQ=255)
          gCTACGACGTTTGAGGGCTTGCCGCCATAAACGGCAGACCGCCGAACGAAAACCACCCAGACGTGTCAGGTTTCCCGGCACCGCGTGGTAATTGAAGTAACCGCTCACCACCCGTGTGAGCCATTGCCCTTGAACCCATCTGGGTTCaag                                                                                                                                       <  1:762558/150‑3 (MQ=255)
               gacgTTTGAGGGCTTGCCGCCATAAACGGCAGACCGCCGAACGAAAACCACCCAGACGTGTCAGGTTTCCCGGCACCGCGTGGTAATTGAAGTAACCGCTCACCACCCGTGTGAGCCATTGCCCTTGAACCCAGATGGGTTCATGACGTc                                                                                                                                  >  1:681196/1‑150 (MQ=255)
                acgTTTGAGGGCTTGCCGCCATAAACGGCAGACCGCCGAACGAAAACCACCCAGACGTGTCAGGTTTCCCGGCACCGCGTGGTAATTGAAGTAACCGCTCACCACCCGTGTGAGCCATTGCCCTTGAACCCAGATGGGTTCATGACGTCg                                                                                                                                 >  2:724381/1‑150 (MQ=255)
                         ggCTTGCCGCCATAAACGGCAGACCGCCGAACGAAAACCACCCAGACGTGTCAGGTTTCCCGGCACCGCGTGGTAATTGAAGTAACCGCTCACCACCCGTGTGAGCCATTGCCCTTGAACCCAGATGGGTTCATGACGTCGGTGTTTCAg                                                                                                                        >  1:117385/1‑150 (MQ=255)
                                 gccATAAACGGCAGACCGCCGAACGAAAACCACCCAGACGTGTCAGGTTTCCCGGCACCGCGTGGTAATTGAAGTAACCGCTCACCACCCGTGTGAGCCATTGCCCTTGAACCCAGATGGGTTCATGACGTCGGTGTTTCAGCTCATccc                                                                                                                <  1:518294/150‑1 (MQ=255)
                                  ccATAAACGGCAGACCGCCGAACGAAAACCACCCAGACGTGTCAGGTTTCCCGGCACCGCGTGGTAATTGAAGTAACCGCTCACCACCCGTGTGAGCCATTGCCCTTTAACCCATCTGGGTTCaagg                                                                                                                                      <  1:839343/127‑4 (MQ=255)
                                  ccATAAACGGCAGACCGCCGAACGAAAACCACCCAGACGTGTCAGGTTTCCCGGCACCGCGTGGTAATTGAAGTAACCGCTCACCACCCGTGTGAGCCATTGCCCTTTAACCCATCTGGGTTCaagg                                                                                                                                      >  2:839343/1‑124 (MQ=255)
                                   cATAAACGGCAGACCGCCGAACGAAAACCACCCAGACGTGTCAGGTTTCCCGGCACCGCGTGGTAATTGAAGTAACCGCTCACCACCCGTGTGAGCCATTGCCCTTGAACCCAGATGGGTTCATg                                                                                                                                       <  1:1144810/125‑1 (MQ=255)
                                   cATAAACGGCAGACCGCCGAACGAAAACCACCCAGACGTGTCAGGTTTCCCGGCACCGCGTGGTAATTGAAGTAACCGCTCACCACCCGTGTGAGCCATTGCCCTTGAACCCAGATGGGTTCATg                                                                                                                                       >  2:1144810/1‑125 (MQ=255)
                                    aTAAACGGCAGACCGCCGAACGAAAACCACCCAGACGTGTCAGGTTTCCCGGCACCGCGTGGTAATTGAAGTAACCGCTCACCACCCGTGTGAGCCATTGCCCTTGAACCCAGATGGGTTCATGACGTCCGTGTTTCAGCTCATCCCGCa                                                                                                             >  2:184526/1‑150 (MQ=255)
                                           gCAGACCGCCGAACGAAAACCACCCAGACGTGTCAGGTTTCCCGGCACCGCGTGGTAATTGAAGTAACCGCTCACCACCCGTGTGAGCCATTGCCCTTGAACCCAGATGGGTTCATGACGTCGGTGTTTCAGCTCATCCCGCATGGCcag                                                                                                      <  1:724381/150‑1 (MQ=255)
                                            cAGACCGCCGAACGAAAACCACCCAGACGTGTCAGGTTTCCCGGCACCGCGTGGTAATTGAAGTAACCGCTCACCACCCGTGTGAGCTATTGCCCTTGAACCCAGATGGGTTCATGACGTCGGTGTTTCAGCTCATCCCGCATGGCcagc                                                                                                     <  2:376010/150‑1 (MQ=255)
                                               aCCGCCGAACGAAAACCACCCAGACGTGTCAGGTTTCCCGGCACCGCGTGGTAATTGAAGTAACCGCTCACCACCCGTGTGAGCCATTGCCCTTGAACCCAGATGGGTTCATGACGTCGGTGTTTCAGCTCATCCCGCATGGCCagcagc                                                                                                  >  2:509991/1‑150 (MQ=255)
                                                  gccgAACGAAAACCACCCAGACGTGTCAGGTTTCCCGGCACCGCGTGGTAATTGAAGTAACCGCTCACCACCCGTGTGAGCCATTGCCCTTGAACCCAGATGGGTTCATGACGTCGGTGTTTCAGCTCATCCCGCATGGCCAGCAGCATc                                                                                               >  2:723716/1‑150 (MQ=255)
                                                                 cccAGACGTGTCAGGTTTCCCGGCACCGCGTGGTAATTGAAGTAACCGCTCACCACCCGTGTGAGCCATTGCCCTTGAACCCAGATGGGTTCATGACGTCGGTGTTTCAGCTCATCCCGCATGGCCAGCAGCATCGCACGCATTCGcttc                                                                                >  2:612854/1‑150 (MQ=255)
                                                                       cGTGTCAGGTTTCCCGGCACCGCGTGGTAATTGAAGTAACCGCTCACCACCCGTGTGAGCCATTGCCCTTGAACCCAGATGGGTTCATGACGTCGGTGTTTCAGCTCATCCCGCATGGCCAGCAGCATCGCACGCATTCGCTTCTTGAcc                                                                          <  1:1163977/150‑1 (MQ=255)
                                                                               gTTTCCCGGCACCGCGTGGTAATTGAAGTAACCGCTCACCACCCGTGTGAGCCATTGCCCTTGAACCCAGATGGGTTCATGACGTCGGTGTTTCAGCTCATCCCGCATGGCCAGCAGCATCGCACGCATTCGCTTCTTGACCGTCAGTCg                                                                  >  2:525820/1‑150 (MQ=255)
                                                                                tttCCCGGCACCGCGTGGTAATTGAAGTAACCGCTCACCACCCGTGTGAGCCATTGCCCTTGAACCCAGATGGGTTCATGACGTCGGTGTTTCAGCTCATCCCGCATGGCCAGCAGCATCGCACGCATTCGCTTCTTGACCGTCAGTCGc                                                                 >  1:1013861/1‑150 (MQ=255)
                                                                                    ccGGCACCGCGTGGTAATTGAAGTAACCGCTCACCACCCGTGTGAGCCATTGCCCTTGAACCCAGATGGGTTCATGACGTCGGTGTTTCAGCTCATCCCGCATGGCCAGCAGCATCGCACGCATTCGCTTCTTGACCGTCa                                                                      <  1:602987/141‑1 (MQ=255)
                                                                                    ccGGCACCGCGTGGTAATTGAAGTAACCGCTCACCACCCGTGTGAGCCATTGCCCTTGAACCCAGATGGGTTCATGACGTCGGTGTTTCAGCTCATCCCGCATGGCCAGCAGCATCGCACGCATTCGCTTCTTGACCGTCa                                                                      >  2:602987/1‑141 (MQ=255)
                                                                                     cGGCACCGCGTGGTAATTGAAGTAACCGCTCACCACCCGTGTGAGCCATTGCCCTTGAACCCAGATGGGTTCATGACGTCGGTGTTTCAGCTCATCCCGCATGGCCAGCAGCATCGCACGCATTCGCTTCTTGACCGTCAGTCGCAGTAt                                                            >  2:881219/1‑150 (MQ=255)
                                                                                         aCCGCGTGGTAATTGAAGTAACCGCTCACCACCCGTGTGAGCCATTGCCCTTGAACCCAGATGGGTTCATGACGTCGGTGTTTCAGCTCATCCCGCATGGCCAGCAGCATCGCACGCATTCGCTTCTTGACCGt                                                                        <  1:237350/134‑1 (MQ=255)
                                                                                         aCCGCGTGGTAATTGAAGTAACCGCTCACCACCCGTGTGAGCCATTGCCCTTGAACCCAGATGGGTTCATGACGTCGGTGTTTCAGCTCATCCCGCATGGCCAGCAGCATCGCACGCATTCGCTTCTTGACCGt                                                                        >  2:237350/1‑134 (MQ=255)
                                                                                         aCCGCGTGGTAATTGAAGTAACCGCTCACCACCCGTGTGAGCCATTGCCCTTGAACCCAGATGGGTTCATGACGTCGGTGTTTCAGCTCATCCCGCATGGCCAGCAGCATCGCACGCATTCGCTTCTTGACCGTCAGTCGCAGTATTTGa                                                        <  1:612854/150‑1 (MQ=255)
                                                                                               tGGTAATTGAAGTAACCGCTCACCACCCGTGTGAGCCATTGCCCTTGAACCCAGATGGGTTCATGACGTCGGTGTTTCAGCTCATCCCGCATGGCCAGCAGCATCGCACGCATTCGCTTCTTGACCGTCAGTCGCAGTATTTGAAACCCa                                                  <  1:881219/150‑1 (MQ=255)
                                                                                                  tAATTGAAGTAACCGCTCACCACCCGTGTGAGCCATTGCCCTTGAACCCAGATGGGTTCATGACGTCGGTGTTTCAGCTCATCCCGCATGGCCAGCAGCATCGCACGCATTCGCTTCTTGACCGTCAGTCGCAGTATTTGAAACCCACCg                                               >  1:455385/1‑150 (MQ=255)
                                                                                                    aTTGAAGTAACCGCTCACCACCCGTGTGAGCCATTGCCCTTGAACCCAGATGGGTTCATGACGTCGGTGTTTCAGCTCATCCCGCATGGCCAGCAGCATCGCACGCATTCGCTTCTTGACCGTCAGTCGCAGTATTTGAAACCCACCGCt                                             >  2:1014922/1‑150 (MQ=255)
                                                                                                        aaGTAACCGCTCACCACCCGTGTGAGCCATTGCCCTTGAACCCAGATGGGTTCATGACGTCGGTGTTTCAGCTCATCCCGCATGGCCAGCAGCATCGCACGCATTCGCTTCTTGACCGTCAGTCGCAGTATTTGAAACCCACCGCTTCTg                                         <  2:1013861/150‑1 (MQ=255)
                                                                                                            aaCCGCTCACCACCCGTGTGAGCCATTGCCCTTGAACCCAGATGGGTTCATGACGTCGGTGTTTCAGCTCATCCCGCATGGCCAGCAGCATCGCACGCATTCGCTTCTTGACCGTCAGTCGCAGTATTTGAAACCCACCGCTTCTGTTgg                                     <  2:681196/150‑1 (MQ=255)
                                                                                                               cGCTCACCACCCGTGTGAGCCATTGCCCTTGAACCCAGATGGGTTCATTACGTCGGTGTTTCAGCTCATCCCGCATGGCCAGCAGCATCGCACGCATTCGCTTCTTGACCGTCAGTCGCAGTATTTGAAACCCACCGCTTCTGTTGGtac                                  >  1:579160/1‑150 (MQ=255)
                                                                                                                  tCACCACCCGTGTGAGCCATTGCCCTTGAACCCAGATGGGTTCATGACGTCGGTGTTTCAGCTCATCCCGCATGGCCAGCAGCATCGCACGCATTCGCTTCTTGACCGTCAGTCGCAGTATTTGAAACCCACCGCTTCTGTTGGtactac                               <  2:579160/150‑1 (MQ=255)
                                                                                                                    accaccCGTGTGAGCCATTGCCCTTGAACCCAGATGGGTTCATGACGTCGGTGTTTCAGCTCATCCCGCATGGCCAGCAGCATCGCACGCATTCGCTTCTTGACCGTCAGTCGCAGTATTTGaa                                                       >  2:765991/1‑124 (MQ=255)
                                                                                                                    accaccCGTGTGAGCCATTGCCCTTGAACCCAGATGGGTTCATGACGTCGGTGTTTCAGCTCATCCCGCATGGCCAGCAGCATCGCACGCATTCGCTTCTTGACCGTCAGTCGCAGTATTTGaa                                                       <  1:765991/124‑1 (MQ=255)
                                                                                                                    accaccCGTGTGAGCCATTGCCCTTGAACCCAGATGGGTTCATGACGTCGGTGTTTCAGCTCATCCCGCATGGCCAGCAGCATCGCACGCATTCGCTTCTTGACCGTCAGTCGCAGTATTTGAAACCCACCGCTTCTGTTGGTACTacaa                             >  1:1157499/1‑150 (MQ=255)
                                                                                                                         ccGTGTGAGCCATTGCCCTTGAACCCAGATGGGTTCATGACGTCGGTGTTTCAGCTCATCCCGCATGGCCAGCAGCATCGCACGCATTCGcttctt                                                                              >  2:616406/1‑96 (MQ=255)
                                                                                                                         ccGTGTGAGCCATTGCCCTTGAACCCAGATGGGTTCATGACGTCGGTGTTTCAGCTCATCCCGCATGGCCAGCAGCATCGCACGCATTCGcttctt                                                                              <  1:616406/96‑1 (MQ=255)
                                                                                                                         ccGTGTGAGCCATTGCCCTTGAACCCAGATGGGTTCATGACGTCGGTGTTTCAGCTCATCCCGCATGGCCAGCAGCATCGCACGCATTCGCTTCTTGACCGTCAGTCGCAGTATTTGAAACCCACCGCTTCTGTTGGTACTACAACAGTg                        >  2:271784/1‑150 (MQ=255)
                                                                                                                         ccGTGTGAGCCATTGCCCTTGAACCCAGATGGGTTCATGACGTCGGTGTTTCAGCTCATCCCGCATGGCCAGCAGCATCGCACGCATTCGCTTCTTGACCGTCAGTCGCAGTATTTGAAACCCACCGCTTCTGTTGGTACTACAACAGTg                        >  1:529033/1‑150 (MQ=255)
                                                                                                                          cGTGTGAGCCATTGCCCTTGACCCCAGATGGGTTCATGACGTCGGTGTTTCAGCTCATCCCGCATGGCCAGCAGCATCGCACGCATTCGCTTCTTGACCGTCAGTCGCAGTATTTGAAACCCACCGCTTCTGTTGGTACTACAACAGTGc                       >  1:37158/1‑150 (MQ=255)
                                                                                                                              tgAGCCATTGCCCTTGAACCCAGATGGGTTCATGACGTCGGTGTTTCAGCTCATCCCGCATGGCCAGCAGCATCGCACGCATTCGCTTCTTGACCGTCAGTCGCAGTATTTGAAACCCACCGCTTCTGTTGGTACTACAACAGTGCGTGa                   >  1:900807/1‑150 (MQ=255)
                                                                                                                                  ccATTGCCCTTGAACCCAGATGGGTTCATGACGTCGGTGTTTCAGCTCATCCCGCATGGCCAGCAGCATCGCACGCATTCGCTTCTTGACCGTCAGTCGCAGTATTTGAAACCCACCGCTTCTGt                                        >  1:1039786/1‑125 (MQ=255)
                                                                                                                                  ccATTGCCCTTGAACCCAGATGGGTTCATGACGTCGGTGTTTCAGCTCATCCCGCATGGCCAGCAGCATCGCACGCATTCGCTTCTTGACCGTCAGTCGCAGTATTTGAAACCCACCGCTTCTGt                                        <  2:1039786/125‑1 (MQ=255)
                                                                                                                                  ccATTGCCCTTGAACCCAGATGGGTTCATGACGTCGGTGTTTCAGCTCATCCCGCATGGCCAGCAGCATCGCACGCATTCGCTTCTTGACCGTCAGTCGCAGTATTTGAAACCCACCGCTTCTGTTGGTACTACAACAGTGCGTGAAGcc               >  2:681992/1‑150 (MQ=255)
                                                                                                                                   cATTGCCCTTGAACCCAGATGGGTTCATGACGTCGGTGTTTCAGCTCATCCCGCATGGCCAGCAGCATCGCACGCATTCGCTTCTTGACCGTCAGTCGCAGTATTTGAAACCCACCGCTTCTGTTGGTACTACAACAGTGCGTGAAGccc              <  1:525820/150‑1 (MQ=255)
                                                                                                                                     ttGCCCTTGAACCCAGATGGGTTCATGACGTCGGTGTTTCAGCTCATCCCGCATGGCCAGCAGCATCGCACGCATTCGCTTCTTGACCGTCAGTCGCAGTATTTGAAACCCACCGCTTCTGTTGGTACTACAACAGTGCGTGAAGCCCAg            <  2:529033/150‑1 (MQ=255)
                                                                                                                                        cccTTGAACCCAGATTGGTTCATGACGTCGGTGTTTCAGCTCATCCCGCATGGCCAGCAGCATCGCACGCATTCGCTTCTTGACCGTCAGTCGCAGTATTTGAAACCCACCGCTTCTGTTGGTACTACAACAGTGCGTGAAGCCCAGGaa         >  2:837143/1‑150 (MQ=255)
                                                                                                                                        cccTTGAACCCAGATGGGTTCATGACGTCGGTGTTTCAGCTCATCCCGCATGGCCAGCAGCATCGCACGCATTCGCTTCTTGACCGTCAGTCGCCGTAATTGAAACCCACCGCTTCTGTTGGTACTACAACAGTGCGTGCTGCCAAggca         >  2:216630/1‑148 (MQ=255)
                                                                                                                                               aCCCAGATGGGTTCATGACGTCGGTGTTTCAGCTCATCCCGCATGGCCAGCAGCATCGCACGCATTCGCTTCTTGACCGTCAGTCGCAGTATTTGAAACCCACCGCTTCTGTTGGTACTACAACAGTGCGTGAAGCCCAGGAAGTCAAAc  <  1:1014922/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                               
GATTACGCTGGCTACGACGTTTGAGGGCTTGCCGCCATAAACGGCAGACCGCCGAACGAAAACCACCCAGACGTGTCAGGTTTCCCGGCACCGCGTGGTAATTGAAGTAACCGCTCACCACCCGTGTGAGCCATTGCCCTTGAACCCAGATGGGTTCATGACGTCGGTGTTTCAGCTCATCCCGCATGGCCAGCAGCATCGCACGCATTCGCTTCTTGACCGTCAGTCGCAGTATTTGAAACCCACCGCTTCTGTTGGTACTACAACAGTGCGTGAAGCCCAGGAAGTCAAAC  >  NC_002947/5039552‑5039844

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑