| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 5,297,289 | A→T | 7.4% | V41E (GTG→GAG) | TpbB ← | diguanylate cyclase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 5,297,289 | 0 | A | T | 7.4% | 149.5 / 2.7 | 52 | V41E (GTG→GAG) | TpbB | diguanylate cyclase |
| Reads supporting (aligned to +/- strand): ref base A (28/20); new base T (2/2); total (30/22) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.23e-01 | |||||||||||
GCCACTTCTTCGGTACTGGCAATCAACGCCAGCGATTCGTTGGCGGCGGCGCTGTCATCGAACACCACAGCCGCTTCTACGGTGTAGTTGATCGAGCGGGCGATCAACTGCAGGTTATGGTTGGCATAGACACGCAGGGCGAGCACGCCCAGCAGGGTG‑AGGGAGATACCCGCCAGGCCAACGGCAAGCAAGGCGACGCTGAGGTGGCCACGGCCCAGCACCGAGCGCAGGGTCGGGCGCACGGTACGCTTGCGGGTCGGCTTCATGGCTGCTCCGCCCGGCGCCGCGACAATT > NC_002947/5297145‑5297438 | gCCACTTCTTCGGTACTGGCAATCAACGCCAGCGATTCGTTGGCGGCGGCGCTGTCATCGAACACCACAGCCGCTTCTACGGTGTAGTTGATCGAGCGGGCGATCAACTGCAGGTTATGGTTGGCATAGACACGCAGGGCGAGCACGccc > 2:475439/1‑150 (MQ=255) cACTTCTTCGGTACTGGCAATCAACGCCAGCGATTCGTTGGCGGCGGCGCTGTCATCGAACACCACAGCCGCTTCTACGGTGTAGTTGATCGAGCGGGCGATCAACTGCAGGTTATGGTTGGCATAGACACGCAGGGCGTGCTCGccctg < 2:712479/150‑3 (MQ=255) tcttcGGTACTGGCAATCAACGCCAGCGATTCGTTGGCGGCGGCGCTGTCATCGAACACCACAGCCGCTTCTACGGTGTAGTTGATCGAGCGGGCGATCAACTGCAGGTTATGGTTGGCATAGACACGCAGGGCGAGCACGCCCAGCCgg < 1:1206216/150‑1 (MQ=255) tcttcGGTACTGGCAATCAACGCCAGCGATTCGTTGGCGGCGGCGCTGTCATCGAACACCACAGCCGCTTCTACGGTGTAGTTGATCGAGCGGGCGATCAACTGCAGGTTATGGTTGGCATAGACACGCAGGGCGAGCACGCCCAGCAgg < 1:1076328/150‑1 (MQ=255) tcttcGGTACTGGCAATCAACGCCAGCGATTCGTTGGCGGCGGCGCTGTCATCGAACACCACAGCCGCTTCTACGGTGTAGTTGATCGAGCGGGCGATCAACTGCAGGTTATGGTTGGCATAGACACGCAGGGCGAGCACGCCCAGCAgg < 1:426471/150‑1 (MQ=255) cttcGGTACTGGCAATCAACGCCAGCGATGCGTTGGCGGCGGCGCTGTCATCGAACACCACAGCCGCTTCTACGGTGTAGTTGATCGAGCGGGCGATCAACTGCAGGTTATGGTTGGCATAGACACGCAGGGCGAGCACGCCCAGCAggg < 2:542907/150‑1 (MQ=255) ggTACTGGCAATCAACGCCAGCGATTCGTTGGCGGCGGCGCTGTCATCGAACACCACAGCCGCTTCTACGGTGTAGTTGATCGAGCGGGCGATCAACTGCAGGTTATGGTTGGCATAGACACGCAGGGCGAGCACGCCCAGCAGGGTG‑Ag < 2:469297/150‑1 (MQ=255) gTACTGGCAATCAACGCCAGCGATTCGTTGGCGGCGGCGCTGTCATCGAACACCACAGCCGCTTCTACGGTGTAGTTGATCGAGCGGGCGATCAACTGCAGGTTATGGTTGGCATAGACACGCAGGGCGAGCACGCCCAGCAGGGTG‑Agg > 1:1126373/1‑150 (MQ=255) aCTGGCAATCAACGCCAGGGATTCGTTGGCGGCGGCGCTGTCATCGAACACCACAGCCGCTTCTACGGTGTAGTTGATCGAGCGGGCGATCATCTGCAGGTTATGGTTGGCATAGACACGCAGGGCGAGCACGCCCAGCAGGGTG‑AGGga < 2:912448/150‑1 (MQ=255) tGGCAATCAACGCCAGCGATTCGTTGGCGGCGGCGCTGTCATCGAACACCACAGCCGCTTCTACGGTGTAGTTGATCGAGCGGGCGATCAACTGCAGGTTATGGTTGGCATAGACACGCAGGGCGAGCACGCCCAGCAGGGTG‑AGGgaga > 2:257797/1‑150 (MQ=255) aaTCAACGCCAGCGATTCGTTGGCGGCGGCGCTGTCATCGAACACCACAGCCGCTTCTACGGTGTAGTTGATCGAGCGGGCGATCAACTGCAGGTTATGGTTGGCATAGACACGCAGGGCGAGCACGCCCAGCAGGGTG‑AGGGAGATAcc > 1:273354/1‑150 (MQ=255) cAGCGATTCGTTGGCGGCGGCGCTGTCATCGAACACCACAGCCGCTTCTACGGTGTAGTTGATCGAGCGGGCGATCAACTGCAGGGTATGGTTGGCATAGACACGCAGGGCGAGCACGCCCAGCAGGGTG‑AGGGAGATACCCGCCAGGcc > 1:944965/1‑150 (MQ=255) cAGCGATTCGTTGGCGGCGGCGCTGTCATCGAACACCACAGCCGCTTCTACGGTGTAGTTGATCGAGCGGCCGATCAACTGCAGGTTATGGTTGGCATCGACACGCAGGACGAGCACGCCCAGCAGGGTG‑GGGGAGATACCCGCCAggaa > 2:130522/1‑148 (MQ=255) cGATTCGTTGGCGGCGGCGCTGTCCTCGGACACCACAGCCGCTTCTACGGTGTAGTTGATCGAGCGGGCGATCAACTGCAGGTTATGGTTGGCATAGACACGCAGGGCGAGCACGCCCAGCAGGGTG‑AGGGAGATACCCGCCAGGCCAAc > 1:337488/1‑150 (MQ=255) cGATTCGTTGGCGGCGGCGCTGTCATCGAACACCACAGCCGCTTCTACGGTGTAGTTGATCGAGCGGGCGATCAACTGCAGGTTATGGTTGGCATAGACACGCAGGGCGAGCACGCCCAGCAGGGTG‑AGGGAGATACCCGCCAGGCCAAc < 2:1126373/150‑1 (MQ=255) cggcggcgCTGTCATCGAACACCACAGCCGCTTCTACGGTGTAGTTGATCGAGCGGGCGATCAACTGCAGGTTATGGTTGGCATAGACACGCAGGGCGAGCACGCCCAGCAGGGTG‑AGGGAGATACCCGCCAGGCCAACGGCAAGCAAgg < 1:257797/150‑1 (MQ=255) tGTCATCGAACACCACAGCCGCTTCTACGGTGTAGTTGATCGAGCGGGCGATCAACTGCAGGTTATGGTTGGCATAGACACGCAGGGCGAGCACGCCCAGCAGGGTG‑AGGGAGATACCCGCCAGGCCAACGGCAAGCAAGGCGACGCTGa > 2:692295/1‑150 (MQ=255) aTCGAACACCACAGCCGCTTCTACGGTGTAGTTGATCGAGCGGGCGATCAACTGCAGGTTATGGTTGGCATAGACACGCAGGGCGAGCACGCCCAGCAGGGTG‑AGGGAGATACCCGCCAGGCCAACGGCAAGCAAGGCGACGCTGAGGTg > 2:898779/1‑150 (MQ=255) tCGAACACCACAGCCGCTTCTACGGTGTAGTTGATCGAGCGGGCGATCAACTGGAGGTTATGGTTGGCATAGACACGCAGGGCGAGCACGCCCAGCAGGGTG‑AGGGAGATACCCGCCAGGCCAACGGCAAGCAAGGCGACGCTGAGGTgg < 1:460909/150‑1 (MQ=255) cacaGCCGCTTCTACGGTGTAGTTGATCGAGCGGGCGATCAACTGCAGGTTATGGTTGGCATAGACACGCAGGGCGAGCACGCCCAGCAGGGTG‑AGGGAGATACCCGCCAGGCCAACGGCAAGCAAGGCGACGCTGAGGTGGCCACGGcc < 1:678748/150‑1 (MQ=255) cacaGCCGCTTCTACGGTGTAGTTGATCGAGCGGGCGATCAACTGCAGGTTATGGTTGGCATAGACACGCAGGGCGAGCACGCCCAGCAGGGTG‑AGGGAGATACCCGCCAGGCCAACGGCAAGCAAGGCGACGCTGAGGTGGCCACGGcc > 1:409514/1‑150 (MQ=255) cacaGCCGCTTCTACGGTGTAGTTGATCGAGCGGGCGATCAACTGCAGGTTATGGTTGGCATAGACACGCAGGGCGAGCACGCCCAGCAGGGTG‑AGGGAGATACCCGCCAGGCCAACGGCAAGCAAGGCGACGCTGAGGTGGCCACGGcc > 1:190751/1‑150 (MQ=255) cacaGCCGCTTCTACGGTGTAGTTGATCGAGCGGGCGATCAACTGCAGGTTATGGTTGGCATAGACACGCAGGGCGAGCACGCCCAGCAGGGTG‑AGGGAGATACCCGCCAGGCCAACGGCAAGCAAGGCGACGCTGAGGTGGCCACGGcc > 2:1190400/1‑150 (MQ=255) acaGCCGCTTCTACGGTGTAGTTGATCGAGCGGGCGATCAACTGCAGGTTATGGTTGGCATAGACACGCAGGGCGAGCACGCCCAGCAgg < 2:96853/90‑1 (MQ=255) acaGCCGCTTCTACGGTGTAGTTGATCGAGCGGGCGAGCAACTGCAGGTTATGGTTGGCATAGACACGCAGGGCGAGCACGCCCAGCAgg > 1:96853/1‑90 (MQ=255) gcGCTGCTACGGTGTAGTCGATCGAGCGGGCGGTCAAGGCCAGGGTGTGGGTGGGATAGATACGCAGGGCGAGCACACCCAGCAGGGTG‑AGGGCGATACCCGCCAGGCCAACGGCAAGGAAGGCGACGCTGAGGTGGTCACGGCCCAGCa > 2:412177/2‑150 (MQ=255) cTACGGTGTAGTTGATCGAGCGGGCGATCAACTGCAGGTTATGGTTGGCATAGACACGCAGGGCGAGCACGCCCAGCAGGGTG‑AGGGAGATACCCGCCAGGCCAACGGCAAGCAAGGCGACGCTGAGGTGGCCACGGCCCAGCACCGAgc < 2:273354/150‑1 (MQ=255) tACGGTGTAGTTGATCGAGCGGGCGATCAACTGCAGGTTATGGTTGGCATAGACACGCAGGGCGAGCACGCCCAGCAGGGTG‑AGGGAGATACCCGCCAGGCCAACGGCAAGCAAgg > 1:1137302/1‑116 (MQ=255) tACGGTGTAGTTGATCGAGCGGGCGATCAACTGCAGGTTATGGTTGGCATAGACACGCAGGGCGAGCACGCCCAGCAGGGTG‑AGGGAGATACCCGCCAGGCCAACGGCAAGCAAgg < 2:1137302/116‑1 (MQ=255) gTTGATCGAGCGGGCGATCAACTGCAGGTTATGGTTGGCATAGACACGCAGGGCGAGCACGCCCAGCAGGGTG‑AGGGAGATACCCGCCAGGCCAACg < 1:5574/97‑1 (MQ=255) gTTGATCGAGCGGGCGATCAACTGCAGGTTATGGTTGGCATAGACACGCAGGGCGAGCACGCCCAGCAGGGTG‑AGGGAGATACCCGCCAGGCCAACg > 2:5574/1‑97 (MQ=255) gTTGATCGAGCGGGCGATCAACTGCAGGTTATGGTTGGCATAGACACGCAGGGCGAGCACGCCCAGCAGGGTG‑AGGGAGATACCCGCCAGGCCAACGGCAAGCAAGGCGACGCTGAGGTGGCCACGGCCCAGCACCGAGCGCAGGGTCgg < 1:692295/150‑1 (MQ=255) tCGAGCGGGCGATCAACTGCAGGTTATGGTTGGCATAGACACGCAGGGCGAGCACGCCCAGCAGGGTG‑AGGGAGTTACCCGCCAGGCCAACGGCAAGCAAGGCGACGATGAGGTGGCCACGGCCCAGCACCGAGCGCAGGGTCGGGCGCa > 1:378653/1‑150 (MQ=255) tCGAGCGGGCGATCAACTGCAGGTTATGGTTGGCATAGACACGCAGGGCGAGCACGCCCAGCAGGGTG‑AGGGAGATACCCGCCAGGCCAACGGCAAGCAAGGCGACGCTGAGGTGGCCACGGCCCAGCACCGAGCGCAGGGTCGGGCGCa > 2:556229/1‑150 (MQ=255) tCGAGCGGGCGATCAACTCCAGGTTATGGTTGGCATAGACACGCAGGGCGAGCACGCCCAGCAGGGTG‑AGGGAGATACCCGCCAGGCCAACGGCAAGCAAGGCGACGCTGAGGTGGCCACGGCCCAGCACCGAGCGCAGGGTCGGGCGCa > 1:537998/1‑150 (MQ=255) gCGGGCGATCAACTGCAGGTTATGGTTGGCATAGACACGCAGGGCGAGCACGCCCAGCAGGGTG‑AGGGAGATACCCGCCAGGCCAACGGCAAGCAAGGCGACGCTGAGGTGGCCACGGCCCAGCACCGAGCGCAGGGTCGGGCGCACGGt > 1:993547/1‑150 (MQ=255) gATCAACTGCAGGTTATGGTTGGCATAGACACGCAGGGCGAGCACGCCCAGCAGGGTG‑AGGGAGATACCCGCCAGGCCAACGGCAAGCAAGGCGACGCTGAGGTGGCCACGGCCCAGCACCGAGCGCAGGGTCGGGCGCACGGTACGCtt > 2:451606/1‑150 (MQ=255) tCAACTGCAGGTTATGGTTGGCATAGACACGCAGGGCGAGCACGCCCAGCAGGGTG‑AGGGAGATACCCGACAGGCCAACGGCAAGCAAGGCGACGCTGAGGTGGCCACGGCCCAGCACCGAGCGCAGGGTCGGGCGCCCGGGACGCTTGc > 2:414201/1‑150 (MQ=255) cTGCAGGTTATGGTTGGCATAGACACGCAGGGCGAGCACGCCCCG‑ACTTTGAACAGGTATACCCGCCAGGCCAACGGCAAGCAAGGCGACGCTGAGGTGGCCACGGCCCAGCACCGAGCGCAGGGTCGGGCGCACGGTACGCTTGCGGGt < 2:337488/150‑1 (MQ=255) gTTATGGTTGGCATAGACACGCAGGGCGAGCACGCCCAGCAGGGTG‑AGGGAGATACCCGCCAGGCCAACGGCAAGCAAGGCGACGCTGAGGTGGCCACGGCCCAGCACCGAGCGCAGGGTCGGGCGCACGGTACGCTTGCGGGTCGGCtt < 1:475439/150‑1 (MQ=255) tATGGTTGGAATAGACACGAAGGGCGAGCACGCCCAGCAGGGTG‑AGGGAGATACCCGCCAGGCCAACGGCAAGCAAGGCGACGCTGAGGTGGCCACGGCCCANCACCGAGCGCAGGGTCGGGCGCACGGTACGCTTGCGGGTCGGCTTCa < 1:414201/150‑1 (MQ=255) ttGGCATAGACACGCAGGGCGAGCACGCCCAGCAGGGTG‑AGGGAGATACCCGCCAGGCCAACGGCAAGCAAGGCGACGCTGAg < 2:76706/83‑1 (MQ=255) ttGGCATAGACACGCAGGGCGAGCACGCCCAGCAGGGTG‑AGGGAGATACCCGCCAGGCCAACGGCAAGCAAGGCGACGCTGAg > 1:76706/1‑83 (MQ=255) ttGGCATAGACACGCAGGGCGAGCACGCCCAGCAGGGTG‑AGGGAGATACCCGCCAGGCCAACGGCAAGCAAGGCGACGCTGAGGTGGCCACGGCCCAGCACCGAGCGCAGGGTCGGGCGCACGGTACGCTTGCGGGTCGGCTTCATGgct > 1:243768/1‑150 (MQ=255) gCATAGACACGCAGGGCGAGCACGCCCAGCAGGGTG‑AGGGAGATACCCGCCAGGCCAACGGCAAGCAAGGCGACGCTGAGGTGGCCACGGCCCa > 1:1097293/1‑94 (MQ=255) gCATAGACACGCAGGGCGAGCACGCCCAGCAGGGTG‑AGGGAGATACCCGCCAGGCCAACGGCAAGCAAGGCGACGCTGAGGTGGCCACGGCCCa < 2:1097293/94‑1 (MQ=255) gCATAGACACGCAGGGCGAGCACGCCCAGCAGGGTG‑AGGGAGATACCCGCCAGGCCAACGGCAAGCAAGGCGACGCTGAGGTGGCCACGGCCCAGCACCGAGCGCAGGGTCGGGCGCACGGTACGCTTGCGGGTCGGCTTCATGgctgct > 2:1068489/1‑150 (MQ=255) aTAGACACGCAGGGCGAGCACGCCCAGCAGGGTG‑AGGGAGATACCCGCCAGGCCAACGGCAAGCAAGGCGACGCTGAGGTGGCCACGGCCCAGCACCGAGCGCAGGGTCGGGCGCACGGTACGCTTGCGGGTCGGCTTCATGGCTGCTcc > 2:750600/1‑150 (MQ=255) aTAGACACGCAGGGCGAGCACGCCCAGCAGGGTG‑AGGGAGATACCCGCCAGGCCAACGGCAAGCAAGGCGACGCTGAGGTGGCCACGGCCCAGCACCGAGCGCAGGGTCGGGCGCACGGTACGCTTGCGGGTCGGCTTCATGGCTGCTcc > 1:286649/1‑150 (MQ=255) tgctgggCGTGCTCGCCCTGCAGGGTG‑AGGGAGATACCCGCCAGGCCAACGGCAAGCAAGGCGACGCTGAGGTGGCCACGGCCCAGCACCGAGCGCAGGGTCGGGCGCACGGTACGCTTGCGGGTCGGCTTCATGGCTGCTCCGCCCGgc > 2:412921/5‑150 (MQ=255) tgggCGTGCTCGCCCTGCAGGGTG‑AGGGAGATACCCGCCAGGCCAACGGCAAGCAAGGCGACGCTGAGGTGGCCACGGCCCAGCACCGAGCGCAGGGt < 2:689813/97‑1 (MQ=255) tgggCGTGCTCGCCCTGCAGGGTG‑AGGGAGATACCCGCCAGGCCAACGGCAAGCAAGGCGACGCTGAGGTGGCCACGGCCCAGCACCGAGCGCAGGGt > 1:689813/2‑98 (MQ=255) aCGCCCAGCAGGGTG‑AGGGAGATACCCGCCAGGCCAACGGCAAGCAAGGCGACGCTGAGGTGGCCACGGCCCAGCACCGAGCGCAGGGTCGGGCGCACGGTACGCTTGCGGGTCGGCTTCATGGCTGCTCCGCCCGGCGCCGCGACAAtt < 2:286649/150‑1 (MQ=255) aCGCCCAGCAGGGTG‑AGGGAGATACCCGCCAGGCCAACGGCAAGCAAGGCGACGCTGAGGTGGCCACGGCCCAGCACCGAGCGCAGGGTCGGGCGCACGGTACGCTTGCGGGTCGGCTTCATGGCTGCTCCGCCCGGCGCCGCGACAAtt < 1:1190400/150‑1 (MQ=255) | GCCACTTCTTCGGTACTGGCAATCAACGCCAGCGATTCGTTGGCGGCGGCGCTGTCATCGAACACCACAGCCGCTTCTACGGTGTAGTTGATCGAGCGGGCGATCAACTGCAGGTTATGGTTGGCATAGACACGCAGGGCGAGCACGCCCAGCAGGGTG‑AGGGAGATACCCGCCAGGCCAACGGCAAGCAAGGCGACGCTGAGGTGGCCACGGCCCAGCACCGAGCGCAGGGTCGGGCGCACGGTACGCTTGCGGGTCGGCTTCATGGCTGCTCCGCCCGGCGCCGCGACAATT > NC_002947/5297145‑5297438 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |