Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,318,049 T→C 8.1% Q458R (CAG→CGG)  ilvI ← acetohydroxybutanoate synthase/acetolactate synthase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,318,0490TC 8.1% 137.6 / 2.8 49Q458R (CAG→CGG) ilvIacetohydroxybutanoate synthase/acetolactate synthase
Reads supporting (aligned to +/- strand):  ref base T (21/24);  new base C (2/2);  total (23/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.50e-01

GATAAAGTCAGGCAGCGACTCGACGTACGAGTGCGAGTGACGACCGTTGTAGGCCATGTCCTGCCACTGGCGGACCATGCCCAACACGCCGTTGTTCAGGTTGACGATCTTCACCGGCAGGCCGTATTGCATGCAGGTGGACAGCTCCTGGATGTTCATCTGGATGCTGCCTTCGCCGGTCACACAGGCCACGTCCTGATCCGGGAAGTTGAGCTTGACGCCCATCGCCGCCGGGAAGCCGAAGCCCATGGTGCCCAGGCCACCGGAGTTGATCCAGCGGTTTGGCTTGTTGAAGCGG  >  NC_002947/5317901‑5318198
                                                                                                                                                    |                                                                                                                                                     
gATAAAGTCAGGCAGCGACTCGACGTACGAGTGCGAGTGACGACCGTTGTAGGCCATGTCCTGCCACTGGCGGACCATGCCCAACACGCCGTTGTTCAGGTTGACGATCTTCACCGGCAGGCCGTATTGCATGCAGGTGGACAGCTCCTg                                                                                                                                                      <  2:442862/150‑1 (MQ=255)
 aTAAAGTCAGGCAGCGACTCGACGTACGAGTGCGAGTGACGACCGTTGTAGGCCATGTCCTGCCACTGGCGGACCATGCCCAACACGCCGTTGTTCAGGTTGACGATCTTCACCGGCAGGCCGTATTGCATGCAGGTGGACAGCTCCTgg                                                                                                                                                     <  1:823961/150‑1 (MQ=255)
   aaaGTCAGGCAGCGACTCGACGTACGAGTGCGAGTGACGACCGTTGTAGGCCATGTCCTGCCACTGGCGGACCATGCCCAACACGCCGTTGTTCAGGTTGACGATCTTCACCGGCAGGCCGTATTGCATGCAGGTGGACAGCTCCTGGac                                                                                                                                                   <  2:56674/150‑2 (MQ=255)
           gCAGCGACTCGACGTACGAGTGCGAGTGACGACCGCTGTAGGCCATGTCCTGCCACTGGCGGACCATGCCCAACACGCCGTTGTTCAGGTTGACGATCTTCACCGGCAGGCCGTATTGCATGCAGGTGGACAGCTCCTGGATGTTCATCt                                                                                                                                           <  2:645509/150‑1 (MQ=255)
                gACTCGACGTACGAGTGCGAGTGACGACCGTTGTAGGCCATGTCCTGCCACTGGCGGACCATGCCCAACACGCCGTTGTTCAGGTTGACGATCTTCACCGGCAGGCCGTATTGCATGCAGGTGGACAGCTCCTGGATGTTCATCTGGAtg                                                                                                                                      >  1:701856/1‑150 (MQ=255)
                               tgcgagtgACGACCGTTGTAGGCCATGTCCTGCCACTGGCGGACCATGCCCAACACGCCGTTGTTCAGGTTGACGATCTTCACCGGCAGGCCGTATTGCATGCAGGTGGACTGCTCCTGGATGTTCATCTGGATGCTGCCTTCGCCGGTc                                                                                                                       <  2:698679/150‑1 (MQ=255)
                                     tgACGACCGTTGTAGGCCATGTCCTGCCACTGGCGGACCATGCCCAACACGCCGTTGTTCAGGTTGACGATCTTCACCGGCAGGCCGTATTGCATGCAGGTGGACAGCTCCTGGATGTTCATCTGGATGCTGCCTTCGCCGGTCACACAg                                                                                                                 <  1:826784/150‑1 (MQ=255)
                                          acCGTTGTAGGCCATGTCCTGCCACTGGCGGACCATGCCCAACACGCCGTTGTTCAGGTTGACGATCTTCACCGGCAGGCCGTATTGCATGCAGGTGGACAGCTCCTGGATGTTCAt                                                                                                                                             <  2:290245/117‑1 (MQ=255)
                                          acCGTTGTAGGCCATGTCCTGCCACTGGCGGACCATGCCCAACACGCCGTTGTTCAGGTTGACGATCTTCACCGGCAGGCCGTATTGCATGCAGGTGGACAGCTCCTGGATGTTCAt                                                                                                                                             >  1:290245/1‑117 (MQ=255)
                                          acCGTTGTAGGCCATGTCCTGCCACTGGCGGACCATGCCCAACACGCCGTTGTTCAGGTTGACGATCTTCACCGGCAGGCCGTATTGCATGCAGGTGGACAGCTCCTGGATGTTCATCTGGATGCTGCCTTCGCCGGTCACACAGGCCAc                                                                                                            <  2:669825/150‑1 (MQ=255)
                                           ccGTTGTAGGCCATGTCCTGCCACTGGCGGACCATGCCCAACACGCCGTTGTTCAGGTTGACGATCTTCACCGGCAGGCCGTATTGCATGCAGGTGGACAGCTCCTGGATGTTCATCTGGATGCTGCCTTCGCCGGTCACACAGGCCACg                                                                                                           <  2:360790/150‑1 (MQ=255)
                                                 tAGGCCATGTCCTGCCACTGGCGGACCATGCCCAACACGCCGTTGTTCAGGTTGACGATCTTCACCGGCAGGCCGTATTGCATGCAGGTGGACAGCTCCTGGATGTTCATCTGGATGCTGCCTTCGCCGGTCACACAGGCCACGTCCTGa                                                                                                     >  1:903079/1‑150 (MQ=255)
                                                 tAGGCCATGTCCTGCCACTGGCGGACCATGCCCAACACGCCGTTGTTCAGGTTGACGATCTTCACCGGCAGGCCGTATTGCATGCAGGTGGACAGCTCCTGGATGTTCATCTGGATGCTGCCTTCGCCGGTCACACAGGCCACGTCCTGa                                                                                                     >  1:423353/1‑150 (MQ=255)
                                                 tAGGCCATGTCCTGCCACTGGCGGACCATGCCCAACACGCCGTTGTTCAGGTTGACGATCTTCACCGACAGGCCGTATTGCATGCTGGTGGACAGCTCCTGGATGTTCATCTGGATGCTGCCTTCGCCGGTCACACAGGCCACGTCCTGa                                                                                                     >  2:738715/1‑150 (MQ=255)
                                                    gCCATGTCCTGCCACTGGCGGACCATGCCCAACACGCCGTTGTTCAGGTTGACGATCTTCACCGGCAGGCCGTATTGCATGCAGGTGGACAGCTCCTGGATGTTCATCTGGATGCTGCCTTCGCCGGTCACACAGGCCACGTCCTGATcc                                                                                                  >  2:529201/1‑150 (MQ=255)
                                                      cATGTCCTGCCACTGGCGGACCATGCCCAACACGCCGTTGTTCAGGTTGACGATCTTCACCGGCAGGCCGTATTGCATGCAGGTGGACAGCTCCTGGATGTTCATCTGGATGCTGCCTTCGCCGGTCACACAGGCCACGTCCTGATCCgg                                                                                                >  1:970271/1‑150 (MQ=255)
                                                         gTCCTGCCACTGGCGGACCATGCCCAACACGCCGTTGTTCAGGTTGACGATCTTCACCGGCAGGCCGTATTGCATGCAGGTGGACAGCTCCTGGATGTTCATCTGGATGCTGCCTTCGCCGGTCACACAGGCCACGTCCTGATCCGGGaa                                                                                             <  1:673878/150‑1 (MQ=255)
                                                            cTGCCACTGGCGGACCATGCCCAACACGCCGTTGTTCAGGTTGACGATCTTCACCGGCAGGCCGTATTGCATGCAGGTGGACAGCTCCTGGATGTTCATCTGGATGCTGCCTTCGCCGGTCACACAGGCCACGTCCTGATCCGGGAAGtt                                                                                          <  2:701856/150‑1 (MQ=255)
                                                              gCCACTGGCGGACCATGCCCAACACGCCGTTGTTCAGGTTGACGATCTTCACCGGCAGGCCGTATTGCATGCAGGTGGACAGCTCCTGGATGTTCATCTGGATGCTGCCTTCGCCGGTCACACAGGCCACGTCCTGATCCGGGAAGTTGa                                                                                        <  1:529201/150‑1 (MQ=255)
                                                                    ggCGGACCATGCCCAACACGCCGTTGTTCAGGTTGACGATCTTCACCGGCAGGCCGTATTGCATGCAGGTGGACAGCTCCTGGATGTTCATCTGGATGCTGCCTTCGCCGGTCACACAGGCCACGTCCTGATCCGGGAAGTTGAGCTTGa                                                                                  <  2:1076469/150‑1 (MQ=255)
                                                                         aCCATGCCCAACACGCCGTTGTTCAGGTTGACGATCTTCACCGGCAGGCCGTATTGCATGCAGGTGGACAGCTCCTGGATGTTCATCTGGATGCTGCCTTCGCCGGTCACACAGGCCACGTCCTGATCCGGGAAGTTGAGCTTGACGccc                                                                             <  2:970271/150‑1 (MQ=255)
                                                                           cATGCCCAACACGCCGTTGTTCAGGTTGACGATCTTCACCGGCAGGCCGTATTGCATGCAGGTGGACAGCTCCTGGATGTTCATCTGGATGCTGCCTTCGCCGGTCACACAGGCCACGTCCTGATCCGGGAAGTTGAGCTTGACGCCCAt                                                                           >  1:913187/1‑150 (MQ=255)
                                                                                         cGTTGTTCAGGTTGACGATCTTCACCGGCAGGCCGTATTGCATGCAGGTGGACAGCTCCTGGATGTTCATCTGGATGCTGCCTTCGCCGGTCACACAGGCCACGTCCTGATCCGGGAAGTTGAGCTTGACGCCCATCGCCGCCGGgaagc                                                             >  2:1189740/1‑150 (MQ=255)
                                                                                          gttgttCAGGTTGACGATCTTCACCGGCAGGCCGTATTGCATGCAGGTGGACAGCTCCTGGATGTTCATCt                                                                                                                                           <  1:585976/71‑1 (MQ=255)
                                                                                          gttgttCAGGTTGACGATCTTCACCGGCAGGCCGTATTGCATGCAGGTGGACAGCTCCTGGATGTTCATCt                                                                                                                                           >  2:585976/1‑71 (MQ=255)
                                                                                             gttCAGGTTGACGATCTTCACCGGCAGGCCGTATTGCATGCAGGTGGACAGCTCCTGGATGTTCATCTGGATGCTGCCTTCGCCGGTCACACAGGCCACGTCCTGATCCGGGAAGATAAGATTAAAGCCCATCTCCGCCGGgaagccgaa                                                         >  1:1169274/1‑150 (MQ=255)
                                                                                              ttCAGGTTGACGATCTTCACCGGCAGGCCGTATTGCATGCAGGTGGACAGCTCCTGGATGTTCATCTGGATGCTGCCTTCGCCGGTCACACAGGCCACGTCCTGATCCGGGAAGTTGAGCTTGACGCCCATCGCCGCCGGgaagccgaag                                                        >  1:815184/1‑150 (MQ=255)
                                                                                                     tGACGATCTTCACCGGCAGGCCGTATTGCATGCAGGTGGACAGCTCCTGGATGTTCATCTGGATGCTGCCTTCGCCGGTCACACAGGCCACGTCCTGATCCGGGAAGTTGAGCTTGACGCCCATCGCCGCCGGGAAGCCGAAGCCCATgg                                                 >  2:743168/1‑150 (MQ=255)
                                                                                                              tCACCGGCAGGCCGTATTGCATGCAGGTGGACAGCTCCTGGATGTTCATCTGGATGCTGCCTTCGCCGGTCACACAGGCCACGTCCTGATCCGGGAAGTTGAGCTTGACGCCCATCGCCGCCGGGAAGCCGAAGCCCATGGTGCCCAGGc                                        <  2:903079/150‑1 (MQ=255)
                                                                                                                  cGGCAGGCCGTATTGCATGCAGGTGGACCGCTCCCGGATGTTCATc                                                                                                                                            >  2:1135332/1‑46 (MQ=255)
                                                                                                                  cGGCAGGCCGTATTGCATGCAGGTGGACAGCTCCCGGATGTTCATc                                                                                                                                            >  2:532516/1‑46 (MQ=255)
                                                                                                                  cGGCAGGCCGTATTGCATGCAGGTGGACAGCTCCCGGATGTTCATc                                                                                                                                            <  1:1135332/46‑1 (MQ=255)
                                                                                                                  cGGCAGGCCGTATTGCATGCAGGTGGACAGCTCCCGGATGTTCATc                                                                                                                                            <  1:532516/46‑1 (MQ=255)
                                                                                                                       ggCCGTATTGCATGCAGGTGGACAGCTCCTGGATGTTCATCTGGATGCTGCCTTCGCCGGTCACACAGGCCACGTCCTGATCCGGGAAGTTGAGCTTGACGCCCATCGCCGCCGGGAAGCCGAAGCCCATGGTGCCCAGGCCACCGGAGt                               >  2:197076/1‑150 (MQ=255)
                                                                                                                        gCCGTATTGCATGCAGGTGGACAGCTCCTGGATGTTCATCTGGATGCTGCCTTCGCCGGTCACACAGGCCACGTCCTGATCCGGGAAGTTGAGCTTGACGCCCATCGCCGCCGGgaagccgaa                                                         <  2:212408/123‑1 (MQ=255)
                                                                                                                        gCCGTATTGCATGCAGGTGGACAGCTCCTGGATGTTCATCTGGATGCTGCCTTCGCCGGTCACACAGGCCACGTCCTGATCCGGGAAGTTGAGCTTGACGCCCATCGCCGCCGGgaagccgaa                                                         >  1:212408/1‑123 (MQ=255)
                                                                                                                        gCCGTATTGCATGCAGGTGGACAGCTCCTGGATGTTCATCTGGATGCTGCCTTCGCCGGTCACACAGGCCACGTCCTGATCCGGGAAGTTGAGCTTGACGCCCATCGCCGCCGGGAAGCCGAAGCCCATGGTGCCCAGGCCACCGGAGtt                              <  1:743168/150‑1 (MQ=255)
                                                                                                                          cGTATTGAATGCAGGTGGACAGCTCCTGGATGTTCATATGGATGCTGCCTTCGCCGGTCACACATGCCACGTCCTGATCCGGGAAGTTGAGCTTGACGCCCATCGCCGCCGGGAAGCCGAAGCCCCTGGTGCCCAGGCCACCGGAGTTGa                            >  2:468489/1‑150 (MQ=255)
                                                                                                                              ttGCATGCAGGTGGACAGCTCCTGGATGTTCATCTGGATGCTGCCTTCGCCGGTCACACAGGCCACGTCCTGATCCGGGAAGTTGAGCTTGACGCCCATCGCCGCCGGGAAGCCGAAGCCCATGGTGCCCAGGCCACCGGAGTTGATCCa                        >  2:542591/1‑150 (MQ=255)
                                                                                                                                      aGGTGGACAGCTCCTGGATGTTCATCTGGATGCTGCCTTCGCCGGTCACACAGGCCACGTCCTGATCCGGGAAGTTGAGCTTGACGCCCATCGCCGCCGGGAAGCCGAAGCCCATGGTGCCCAGGCCACCGGa                                 <  2:1137438/133‑1 (MQ=255)
                                                                                                                                      aGGTGGACAGCTCCTGGATGTTCATCTGGATGCTGCCTTCGCCGGTCACACAGGCCACGTCCTGATCCGGGAAGTTGAGCTTGACGCCCATCGCCGCCGGGAAGCCGAAGCCCATGGTGCCCAGGCCACCGGa                                 >  1:1137438/1‑133 (MQ=255)
                                                                                                                                      aGGTGGACAGCTCCTGGATGTTCATCTGGATGCTGCCTTCGCCGGTCACACAGGCCACGTCCTGATCCGGGAAGTTGAGCTTGACGCCCATCGCCGCCGGGAAGCCGAAGCCCATGGTGCCCAGGCCACCGGAGTTGATCCAGCGGTTTg                >  2:21115/1‑150 (MQ=255)
                                                                                                                                        gTGGACAGCTCCTGGATGTTCATCTGGATGCTGCCTTCGCCGGTCACACAGGCCACGTCCTGATCCGGGAAGTTGAGCTTGACGCCCATCGCCGCCGGGAAGCCGAAGCCCATGGTGCCCAGGCCACCGGAGTTGATCCAGCGGTTTGGc              <  2:913187/150‑1 (MQ=255)
                                                                                                                                          ggACAGCTCCTGGATGTTCATCTGGATGCTGCCTTCGCCGGTCACACAGGCCACGTCCTGATCCGGGAAGTTGAGCTTGa                                                                                  >  2:1186582/1‑80 (MQ=255)
                                                                                                                                          ggACAGCTCCTGGATGTTCATCTGGATGCTGCCTTCGCCGGTCACACAGGCCACGTCCTGATCCGGGAAGTTGAGCTTGa                                                                                  <  1:1186582/80‑1 (MQ=255)
                                                                                                                                          ggACAGCTCCTGGATGTTCATCTGGATGCTGCCTTCGCCGGTCACACAGGCCACGTCCTGATCCGGGAAGTTGAGCTTGACGCCCATCGCCGCCGGGAAGCCGAAGCCCATGGTGCCCAGGCCACCGGAGTTGATCCAGCGGTTTGGCtt            <  2:423353/150‑1 (MQ=255)
                                                                                                                                             cAGCTCCTGGATGTTCATCTGGATGCTGCCTTCGCCGGTCACACAGGCCACGTCCTGATCCGGGAAGTTGAGCTTGACGCCCATCGCCGCCGGGAAGCCGAAGCCCATGGTGCCCAGGCCACCGGAGTTGATCCAGCGGTTTGGCttgtt         <  1:738715/150‑1 (MQ=255)
                                                                                                                                              aGCTCCTGGATGTTCATCTGGATGCTGCCTTCGCCGGTCACACAGGCCACGTCCTGATCCGGGAAGTTGAGCTTGACGCCCATCGCCGCCGGGAAGCCGAAGCCCATGGTGCCCAGGCCACCGGAGTTGATCCAGCGGTTTGGCttgttg        >  1:14495/1‑150 (MQ=255)
                                                                                                                                                cTCCTGGATGTTCATCTGGATGCTGCCTTCGCCGGTCACACAGGCCACGTCCTGATCCGGGAAGTTGAGCTTGACGCCCATCGCCGCCGGGAAGCCGAAGCCCATGGTGCCCAGGCCACCGGAGTTGATCCAGCGGTTTGGCTTGTTGaa      <  1:197076/150‑1 (MQ=255)
                                                                                                                                                    tGGATGTTCATCTGGATGCTGCCTTCGCCGGTCACACAGGCCACGTCCTGATCCGGGAAGTTGAGCTTGACGCCCATCGCCGCCGGGAAGCCGAAGCCCATGGTGCCCAGGCCACCGGAGTTGATCCAGCGGTTTGGCTTGTTGAAGCgg  <  1:1189740/150‑1 (MQ=255)
                                                                                                                                                    tGGATGTTCATCTGGATGCTGCCTTCGCCGGTCACACAGGCCACGTCCTGATCCGGGAAGTTGAGCTTGACGCCCATCGCCGCCGGGAAGCCGAAGCCCATGGTGCCCAGGCCACCGGAGTTGATCCAGCGGTTTGGCTTGTTGAAGCgg  <  2:815184/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
GATAAAGTCAGGCAGCGACTCGACGTACGAGTGCGAGTGACGACCGTTGTAGGCCATGTCCTGCCACTGGCGGACCATGCCCAACACGCCGTTGTTCAGGTTGACGATCTTCACCGGCAGGCCGTATTGCATGCAGGTGGACAGCTCCTGGATGTTCATCTGGATGCTGCCTTCGCCGGTCACACAGGCCACGTCCTGATCCGGGAAGTTGAGCTTGACGCCCATCGCCGCCGGGAAGCCGAAGCCCATGGTGCCCAGGCCACCGGAGTTGATCCAGCGGTTTGGCTTGTTGAAGCGG  >  NC_002947/5317901‑5318198

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑