| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 5,346,315 | C→A | 13.9% | V555V (GTC→GTA) | acsA‑II → | acetyl‑CoA synthetase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 5,346,315 | 0 | C | A | 13.9% | 125.7 / 8.4 | 50 | V555V (GTC→GTA) | acsA‑II | acetyl‑CoA synthetase |
| Reads supporting (aligned to +/- strand): ref base C (18/24); new base A (3/4); total (22/28) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TACTGGATCACCGGGCGCATCGACGATGTGATCAACGTGTCCGGGCACCGCATCGGCACCGCTGAAGTGGAAAGTGCACTGGTGCTGCATGACAGCGTGGCCGAAGCCGCGGTGGTCGGCTACCCCCACGACCTCAAGGGCCAAGGCGTCTACGCCTTCGTCACGACCATGAATGGCGTGACACCAGATGACACGCTCAAGGCCGAGCTGCTGGCCCTGGTCAGCAAGGAAATCGGCAGCTTCGCCAAGCCTGAGCTGATTCAGTGGGCGCCAGCGCTACCCAAGACGCGCTCAGGC > NC_002947/5346166‑5346462 | tactGGATCACCGGGCGCATCGACGATGTGATCAACGTGTCCGGGCACCGCATCGGCACCGCTGAAGTGGAAAGTGCACTGGTGCTGCATGACAGCGTGGCCGAAGCCGCGGTGGTCGGCTACCCCCACGACCTCAAGGGCCAAGGCGTc > 2:422342/1‑150 (MQ=255)tactGGATCACCGGGCGCATCGACGATGTGATCAACGTGTCCGGGCACCGCATCGGCACCGCTGAAGTGGAAAGTGCACTGGTGCTGCATGACAGCGTGGCCGAAGCCGCGGTGGTCGGCTACCCCCACGACCTCAAGGGCCAAGGCGTc > 1:861533/1‑150 (MQ=255) ctGGATCACCGGGCGCATCGACGATGTGATCAACGTGTCCGGGCACCGCATCGGCACCGCTGAAGTGGAAAGTGCACTGGTGCTGCATGACAGCGTGGCCGAAGCCGCGGTGGTCGGCTACCCCCACGACCTCAAGGGCCAAGGCGTCTa > 2:506342/1‑150 (MQ=255) gATCACCGGGCGCATCGACGATGTGATCAACGTGTCCGGGCACCGCATCGGCACCGCTGAAGTGGAAAGTGCACTGGTGCTGCATGACAGCGTGGCCGAAGCCGCGGTGGTCGGCTACCCCCACGACCTCAAGGGCCAAGGCGTc < 2:447929/145‑1 (MQ=255) gATCACCGGGCGCATCGACGATGTGATCAACGTGTCCGGGCACCGCATCGGCACCGCTGAAGTGGAAAGTGCACTGGTGCTGCATGACAGCGTGGCCGAAGCCGCGGTGGTCGGCTACCCCCACGACCTCAAGGGCCAAGGCGTc > 1:447929/1‑145 (MQ=255) aTCACCGGGCGCATCGACGATGTGATCAACGTGTCCGGGCACCGCATCGGCACCGCTGAAGTGGAAAGTGCACTGGTGCTGCATGACAGCGTGGCCGAAGCCGCGGTGGTCGGCTACCCCCACGACCTCAAGGGCCAAGGCGTAGACGcc < 2:474023/150‑1 (MQ=255) tCACCGGGCGCATCGACGATGTGATCAACGTGTCCGGGCACCGCATCGGCACCGCTGAAGTGGAAAGTGCACTGGTGCTGCATGACAGCGTGGCCGAAGCCGCGGGTGCCGACGACCCCCACGAGCTGATGGGCCAAGGCGTATACGCCt > 2:198128/1‑150 (MQ=255) gcATCGACGATGTGATCAACGTGTCCGGGCACCGCATCGGCACCGCTGAAGTGGAAAGTGCACTGGTGCTGCATGACAGCGTGGCCGAAGCCGCGGTGGTCGGCTACCCCCACGACCTCAAGGGCCAAGGCGTCTACGCCTTCGTCACGa < 2:779853/150‑1 (MQ=255) tgATCAACGTGTCCGGGCACCGCATCGGCACCGCTGAAGTGGAAAGTGCACTGGTGCTGCATGACAGCGTGGCCGAAGCCGCGGTGGTCGGCTACCCCCACGACCTCAAGGGCCAAGGCGTCTACGCCTTCGTCACGACCATGAATGGCg < 1:506342/150‑1 (MQ=255) aTCAACGTGTCCGGGCACCGCATCGGCACCGCTGAAGTGGAAAGTGCACTGGTGCTGCATGACGGCGTGGCCGAAGCCGCGGTGGTCGGCTACCCCCACGACCTCAAGGGCCAAGGCGTAGACGCCttggc > 2:256965/1‑128 (MQ=255) aTCAACGTGTCCGGGCACCGCATCGGCACCGCTGAAGTGGAAAGTGCACTGGTGCTGCATGACGGCGTGGCCGAAGCCGCGGTGGTCGGCTACCCCCACGACCTCAAGGGCCAAGGCGTAGACGCCttggc < 1:256965/131‑4 (MQ=255) ttagcGTGTCCCGGCACCGCATCGGCACCGCTGAAGTGGAAAGTGCACTGGTGCTGCATGCCAGCGTGGCCGAAGCCGCGGTGGTCGGCTACCCCCACGACCTCAATGGCCAAGGCGTCTACTCCTTCGTCACGACCATGAATGGCGTGa < 2:187326/146‑1 (MQ=255) aaCGTGTCCGGGCACCGCATCGGCACCGCTGAAGTGGAAAGTGCACTGGTGCTGCATGACAGCGTGGCCGAAGCCGCGGTGGTCGGCTACCCCCACGACCTCAAGGGCCAAGGCGTCTACGCCTTCGTCACGACCATGAATGGCGTGaca > 1:796046/1‑150 (MQ=255) gtgtCCGGGCACCGCATCGGCACCGCTGAAGTGGAAAGTGCACTGGTGCTGCATGACAGCGTGGCCGAAGCCGCGGTGGTCGGCTACCCCCACGACCTCAAGGGCCAAGGCGTCTACGCCTTCGTCACGACCATGAATGGCGTGACACCa < 1:1042548/150‑1 (MQ=255) gtCCGGGCACCGCATCGGCACCGCTGAAGTGGAAAGTGCACTGGTGCTGCATGACAGCGTGGCCGAAGCCGCGGTGGTCGGCTACCCCCACGACCTCAAGGGCCAAGGCGTCTACGCCTTCGTCACGACCATGAATGGCGTGACACCAGa < 2:844302/150‑1 (MQ=255) tCCGGGCACCGCATCGGCACCGCTGAAGTGGAAAGTGCACTGGTGCTGCATGACAGCGTGGCCGAAGCCGCGGTGGTCGGCTACCCCCACGACCTCAAGGGCCAAGGCGTCTACGCCTTCGTCACGACCATGAATGGCGTGACACCAGAt > 2:1181526/1‑150 (MQ=255) aCCGCATCGGCACCGCTGAAGTGGAAAGTGCACTGGTGCTGCATGACAGCGTGGCCGAAGCCGCGGTGGTCGGCTACCCCCACGACCTCAAGGGCCAAGGCGTCTACGCCTTCGTCACGACCATGAATGGCGTGACACCAGATGACACGc > 1:961830/1‑150 (MQ=255) cGCATCGGCACCGCTGAAGTGGAAAGTGCACTGGTGCTGCATGACAGCGTGGCCGAAGCCGCGGTGGTCGGCTACCCCCACGACCTCAAGGGCCAAGGCGTc > 1:1062309/1‑102 (MQ=255) cGCATCGGCACCGCTGAAGTGGAAAGTGCACTGGTGCTGCATGACAGCGTGGCCGAAGCCGCGGTGGTCGGCTACCCCCACGACCTCAAGGGCCAAGGCGTc < 2:1062309/102‑1 (MQ=255) gCATCGGCACCGCTGAAGTGGAAAGTGCACTGGTGCTGCATGACAGCGTGGCCGAAGCCGCGGTGGTCGGCTACCCCCACGACCTCAAGGGCCAAGGCGTCTACGCCTTCGTCACGACCATGAATGGCGTGa > 2:904850/1‑132 (MQ=255) gCATCGGCACCGCTGAAGTGGAAAGTGCACTGGTGCTGCATGACAGCGTGGCCGAAGCCGCGGTGGTCGGCTACCCCCACGACCTCAAGGGCCAAGGCGTCTACGCCTTCGTCACGACCATGAATGGCGTGa < 1:904850/132‑1 (MQ=255) aTCGGCACCGCTGAAGTGGAAAGTGCACTGGTGCTGCATGACAGCGTGGCCGAAGCCGCGGTGGTCGGCTACCCCCACGACCTCAAGGGCCAAGGCGTCTACGCCTTCGTCACGACCATGAATGGCGTGACACCAGATGACACGCTCAAg < 2:926929/150‑1 (MQ=255) cGGCACCGCTGAAGTGGAAAGTGCACTGGTGCTGCATGACAGCGTGGCCGAAGCCGCGGTGGTCGGCTACACCCACGACCTCAAGAGCCAAGGCGTGTAAGGAAACGGCGATACCAGGAGTGTCGGTACACGAGATGACACGCTAAAggg > 2:919149/1‑149 (MQ=255) ggCACCGCTGAAGTGGAAAGTGCACTGGTGCTGCATGACAGCGTGGCCGAAGCCGCGGTGGTCGGCTACCCCCACGACCTCAAGGGCCAAGGCGTCTACGCCTTCGTCACGACCATGAATGGCGTGACACCAGATGACACGCTCAAGGcc > 1:809773/1‑150 (MQ=255) gCACCGCTGAAGTGGAAAGTGCACTGGTGCTGCATGACAGCGTGGCCGAAGCCGCGGTGGTCGGCTACCCCCACGACCTCAAGGGCCAAGGCGTAGACGCCttgg > 2:895905/1‑103 (MQ=255) gCACCGCTGAAGTGGAAAGTGCACTGGTGCTGCATGACAGCGTGGCCGAAGCCGCGGTGGTCGGCTACCCCCACGACCTCAAGGGCCAAGGCGTAGACGCCttgg < 1:895905/105‑3 (MQ=255) gCACCGCTGAAGTGGAAAGTGCACTGGTGCTGAATGACAGCGTGGCCGAAGCCGCGGTGGTCGGCTACCCCCACGACCTCAAGGGCCAAGGCGTCTACGCCTTCGTCACGACCATGAATGGCGTGACACAAGATGACAAGCTCAAGGACg > 1:485187/1‑150 (MQ=255) aCCGCTGAAGTGGAAAGTGCACTGGTGCTGCATGACAGCGTGGCCGAAGCCGCGGTGGTCGGCTACCCCCACGACCTCAAGGGCCAAGGCGTCTACGCCTTCGTCACGACCATGAATGGCGTGa < 1:285282/124‑1 (MQ=255) aCCGCTGAAGTGGAAAGTGCACTGGTGCTGCATGACAGCGTGGCCGAAGCCGCGGTGGTCGGCTACCCCCACGACCTCAAGGGCCAAGGCGTCTACGCCTTCGTCACGACCATGAATGGCGTGa > 2:285282/1‑124 (MQ=255) tGAAGTGGAAAGTGCACTGGTGCTGCATGACAGCGTGGCCGAAGCCGCGGTGGTCGGCTACCCCCACGACCTCACGACCAAAGGCGTCTACGCCACCGGTTGCTTGACCAGTGTGGTGGCACCAGATGACACGCTCAAGGCCGAgctgct < 1:812341/150‑1 (MQ=255) tGGAAAGTGCACTGGTGCTGCATGACAGCGTGGCCGAAGCCGCGGTGGTCGGCTACCCCCACGACCTCAAGGGCCAAGGCGTCTACGCCTTCGTCACGACCATGAATGGCGTGACACCAGATGACACGCTCAAGGCCGAGCTGCTGGccc < 1:1181526/150‑1 (MQ=255) gAAAGTGCACTGGTGCTGCATGACAGCGTGGCCGAAGCCGCGGTGGTCGGCTACCCCCACGACCTCAAGGGCCAAGGCGTCTACGCCTTCGTCACGACCATGAATGGCGTGACACCAGATGACACTCTCAAGGCCGAGCTGCTGGCCCTg > 2:176285/1‑150 (MQ=255) gTGCACTGGTGCTGCATGACAGCGTGGCCGAAGCCGAGGTGACTGGCTACCCCCACGACCTCAAGGGCCAAGGCGTCTACGCCTTCGTCACGACCATGAATGGCGTGACACCAGATGACACGCTCAAGGCCGAGCTGCTGGCCCTGGTCa < 1:919149/150‑1 (MQ=255) aCTGGGGCTGCATGACAGGGTGGCCGAAGCCGCGGTGGTCGGCTACCCTCACGACCTCAAGGGCCAAGGCGTCTACGCCTTCGTCACGACCATGAATGGCGTGACACCAGATGACACGCTCAAGGCCGAGCTGCTGGCCCTGGTCAGCaa < 2:624362/150‑1 (MQ=255) cTGGTGCTGCATGACAGCGTGGCCGAAGCCGCGGTGGTCGGCTACCCCCACGACCTCAAGGGCCAAGGCGTCTACGCCTTCGTCACGACCATGAATGGCGTGACACCAGATGACACGCTCAAGGCCGAGCTGCTGGCCCTGGTCAGCAAg < 1:176285/150‑1 (MQ=255) gTGCTGCATGACAGCGTGGCCGAAGCCGCGGTGGTCGGCTACCCCCACGACCTCAAGGGCCAAGGCGTCTACGCCTTCGTCACGACCATGAATGGCGTGACACCAGATGACACGCTCAAGGCCGAGCTGCTGGCCCTGGTCAGCAAGGaa > 1:106100/1‑150 (MQ=255) cttcATTACATCGTGGCCGAAGCCGCGGTGGTCGGCTACCCCCACGACCTCAAGGGCCAAGGCGTCTACGCCTTCGTCACGACCATGAATGGCGTGACACCAGATGACACGCTCAAGGCCGAGCTGCTGGCCCTGGTCAGCAAGGAAATc < 2:485187/147‑1 (MQ=255) tgcATGACAGCGTGGCCGAAGCCGCGGTGGTTGGCTACCCCCACGACCTCAAGGGCCAAGGCGTCTACGCCTTCGTCACGACCATGAATGGCGTGACACCAGATGACACGCTCAAGGCCGAGCTGCTGGCCCTGGTCAGCAAGGAAATCg < 1:607086/150‑1 (MQ=255) tgcATGACAGCGTGGCCGAAGCCGCGGTGGTCGGCTACCCCCACGACCTCAAGGGCCAAGGCGTCTACGCCTTCGTCACGACCATGAATGGCGTGACACCAGATGACACGCTCAAGGCCGAGCTGCTGGCCCTGGTCAGCAAGGAAATCg < 2:961830/150‑1 (MQ=255) cATGACAGCGTGGCCGAAGCCGCGGTGGTCGGCTACCCCCACGACCTCAAGGGCCAAGGCGTCTACGCCTTCGTCACGACCATGAATGGCGTGACACCAGATGACACGCTCAAGGCCGAGCTGCTGGCCCTGGTCAGCAAGGAAATCGGc > 1:14007/1‑150 (MQ=255) aGCGTGGCCGAAGCCGCGGTGGTCGGCTACCCCCACGACCTCAAGGGCCAAGGCGTCTACGCCTTCGTCACGACCATGAATGGCGTGACACCAGATGa < 2:166013/98‑1 (MQ=255) aGCGTGGCCGAAGCCGCGGTGGTCGGCTACCCCCACGACCTCAAGGGCCAAGGCGTCTACGCCTTCGTCACGACCATGAATGGCGTGACACCAGATGa > 1:166013/1‑98 (MQ=255) ccGAAGCCGCGGTGGTCGGCTACCCCCACGACCTCAAGGGCCAAGGCGTCTACGCCTTCGTCa < 1:1131765/63‑1 (MQ=255) ccGAAGCCGCGGTGGTCGGCTACCCCCACGACCTCAAGGGCCAAGGCGTCTACGCCTTCGTCa > 2:1131765/1‑63 (MQ=255) cGAAGCCGCGGTGGTCGGCTGCCCCCACGACCTCAAGGGCCAAGGCGTCTACGCCTTCGTCACGACCATGAATGGCGTGACACCAGATGACACGCTCAAGGCCGAGCTGCTGGCCCTGGt < 1:624515/120‑1 (MQ=255) cGAAGCCGCGGTGGTCGGCTACCCCCACGACCTCAAGGGCCAAGGCGTCTACGCCTTCGTCACGACCATGAATGGCGTGACACCAGATGACACGCTCAAGGCCGAGCTGCTGGCCCTGGt > 2:624515/1‑120 (MQ=255) aaGCCGCGGTGGTCGGCTACCCCCACGACCTCAAGGGCCAAGGCGTCTACGCCTTCGTCACGACCATGAATGGCGTGACACCAGATGACACGCTCAAGGCCGAGCTGCTGGCCCTGGTCAGCAAGGAAATCGGCAGCTTCGCCAAGCCTG < 1:422342‑M1/150‑1 (MQ=255) gtCGGCTACCCCCACGACCTCAAGGGCCAAGGCGTCTACGCCTTCGTCACGACCATGAATGGCGTGa < 1:42433/67‑1 (MQ=255) gtCGGCTACCCCCACGACCTCAAGGGCCAAGGCGTCTACGCCTTCGTCACGACCATGAATGGCGTGa > 2:42433/1‑67 (MQ=255) cTACCCCCACGACCTCAAGGGCCAAGGCGTCTACGCCTTCGTCACGACCATGAATGGCGTGACACCAGATGACACGCTCAAGGCCGAGCTGCTGGCCCTGGTCAGCAAGGAAATCGGCAGCTTCGCCAAGCCTGAGCTGATTCAGTGGgc < 2:809773/150‑1 (MQ=255) ccccACGACCTCAAGGGCCAAGGCGTCTACGCCTTCGTCACGACCATGAATGGCGTGACACCAGATGACACGCTCAAGGCCGAGCTGCTGGCCCTGGTCAGCAAGGAAATCGGCAGCTTCGCCAAGCCTGAGCTGATTCAGTGGGCGCCa < 2:124383/150‑1 (MQ=255) ccACGACCTCAAGGGCCAAGGCGTCTACGCCTTCGTCACGACCATGAATGGCGTGACACCAGATGACACGCTCAAGGCCGAGCTGCTGGCCCTGGTCAGCAAGGAAATCGGCAGCTTCGCCAAGCCTGAGCTGATTCAGTGGGCGCCAgc > 1:325394/1‑150 (MQ=255) tCAAGGGCCAAGGCGTCTACGCCTTCGTCACGACCATGAATGGCGTGACACCAGATGACACTCTCAAGGCCGAGCTGCCGGTCCTGGTCAGCAAGGAAATCGGCAGCTTCGCCAAGCCTGAGCTGACGCAGTGTGCGCCAGCGCTACCCa > 1:327083/1‑150 (MQ=255) ggCCAAGGCGTCTACGCCTTCGTCATGACCATGAATGGCCTGACACCAGATGACACGCTCAAGGCCGAGCTGCTGGCCTTGGTCAGCAAGGAAATCGGCAGCTCCCCAAAGCCTGAGCTGATTCAGTGGGCGCAAGCGCTACCCAAGAcg < 2:233430/150‑1 (MQ=255) cgaaggCGTAGACGCCTTCGTCAGGACCATGAATGGCGTGACACCAGATGACACGCTCAAGGCCGAGCTGCTGGCCCTGGTCAGCAAGGAAATCGGCAGCTTCGCCAAGACTGAGCTGATTCAGTGGGCGCCAGCGCTACCCAAGACGCg < 2:72294‑M2/148‑2 (MQ=255) gTCTACGCCTTCGTCACGACCATGAATGGCGTGACACCAGATGACACGCTCAAGGCCGAGCTGCTGGCCCTGGTCAGCAAGGAAATCGGCAGCTTCGCCAAGCCTGAGCTGATTCAGTGGGCGCCAGCGCTACCCAAGACGCgctcaggc > 2:796046‑M2/1‑142 (MQ=255) | TACTGGATCACCGGGCGCATCGACGATGTGATCAACGTGTCCGGGCACCGCATCGGCACCGCTGAAGTGGAAAGTGCACTGGTGCTGCATGACAGCGTGGCCGAAGCCGCGGTGGTCGGCTACCCCCACGACCTCAAGGGCCAAGGCGTCTACGCCTTCGTCACGACCATGAATGGCGTGACACCAGATGACACGCTCAAGGCCGAGCTGCTGGCCCTGGTCAGCAAGGAAATCGGCAGCTTCGCCAAGCCTGAGCTGATTCAGTGGGCGCCAGCGCTACCCAAGACGCGCTCAGGC > NC_002947/5346166‑5346462 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |