Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,346,316 T→G 12.0% Y556D (TAC→GAC)  acsA‑II → acetyl‑CoA synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,346,3160TG12.0% 118.1 / 10.1 50Y556D (TAC→GAC) acsA‑IIacetyl‑CoA synthetase
Reads supporting (aligned to +/- strand):  ref base T (20/24);  new base G (2/4);  total (22/28)
Fisher's exact test for biased strand distribution p-value = 6.83e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.88e-01

CTGGATCACCGGGCGCATCGACGATGTGATCAACGTGTCCGGGCACCGCATCGGCACCGCTGAAGTGGAAAGTGCACTGGTGCTGCATGACAGCGTGGCCGAAGCCGCGGTGGTCGGCTACCCCCACGACCTCAAGGGCCAAGGCGTCTACGCCTTCGTCACGACCATGAATGGCGTGACACCAGATGACACGCTCAAGGCCGAGCTGCTGGCCCTGGTCAGCAAGGAAATCGGCAGCTTCGCCAAGCCTGAGCTGATTCAGTGGGCGCCAGCGCTACCCAAGACGCGCTCAGGC  >  NC_002947/5346168‑5346462
                                                                                                                                                    |                                                                                                                                                  
ctGGATCACCGGGCGCATCGACGATGTGATCAACGTGTCCGGGCACCGCATCGGCACCGCTGAAGTGGAAAGTGCACTGGTGCTGCATGACAGCGTGGCCGAAGCCGCGGTGGTCGGCTACCCCCACGACCTCAAGGGCCAAGGCGTCTa                                                                                                                                                   >  2:506342/1‑150 (MQ=255)
    aTCACCGGGCGCATCGACGATGTGATCAACGTGTCCGGGCACCGCATCGGCACCGCTGAAGTGGAAAGTGCACTGGTGCTGCATGACAGCGTGGCCGAAGCCGCGGTGGTCGGCTACCCCCACGACCTCAAGGGCCAAGGCGTAGACGcc                                                                                                                                               <  2:474023/150‑1 (MQ=255)
     tCACCGGGCGCATCGACGATGTGATCAACGTGTCCGGGCACCGCATCGGCACCGCTGAAGTGGAAAGTGCACTGGTGCTGCATGACAGCGTGGCCGAAGCCGCGGGTGCCGACGACCCCCACGAGCTGATGGGCCAAGGCGTATACGCCt                                                                                                                                              >  2:198128/1‑150 (MQ=255)
              gcATCGACGATGTGATCAACGTGTCCGGGCACCGCATCGGCACCGCTGAAGTGGAAAGTGCACTGGTGCTGCATGACAGCGTGGCCGAAGCCGCGGTGGTCGGCTACCCCCACGACCTCAAGGGCCAAGGCGTCTACGCCTTCGTCACGa                                                                                                                                     <  2:779853/150‑1 (MQ=255)
                          tgATCAACGTGTCCGGGCACCGCATCGGCACCGCTGAAGTGGAAAGTGCACTGGTGCTGCATGACAGCGTGGCCGAAGCCGCGGTGGTCGGCTACCCCCACGACCTCAAGGGCCAAGGCGTCTACGCCTTCGTCACGACCATGAATGGCg                                                                                                                         <  1:506342/150‑1 (MQ=255)
                            aTCAACGTGTCCGGGCACCGCATCGGCACCGCTGAAGTGGAAAGTGCACTGGTGCTGCATGACGGCGTGGCCGAAGCCGCGGTGGTCGGCTACCCCCACGACCTCAAGGGCCAAGGCGTAGACGCCttggc                                                                                                                                          >  2:256965/1‑128 (MQ=255)
                            aTCAACGTGTCCGGGCACCGCATCGGCACCGCTGAAGTGGAAAGTGCACTGGTGCTGCATGACGGCGTGGCCGAAGCCGCGGTGGTCGGCTACCCCCACGACCTCAAGGGCCAAGGCGTAGACGCCttggc                                                                                                                                          <  1:256965/131‑4 (MQ=255)
                             ttagcGTGTCCCGGCACCGCATCGGCACCGCTGAAGTGGAAAGTGCACTGGTGCTGCATGCCAGCGTGGCCGAAGCCGCGGTGGTCGGCTACCCCCACGACCTCAATGGCCAAGGCGTCTACTCCTTCGTCACGACCATGAATGGCGTGa                                                                                                                      <  2:187326/146‑1 (MQ=255)
                               aaCGTGTCCGGGCACCGCATCGGCACCGCTGAAGTGGAAAGTGCACTGGTGCTGCATGACAGCGTGGCCGAAGCCGCGGTGGTCGGCTACCCCCACGACCTCAAGGGCCAAGGCGTCTACGCCTTCGTCACGACCATGAATGGCGTGaca                                                                                                                    >  1:796046/1‑150 (MQ=255)
                                  gtgtCCGGGCACCGCATCGGCACCGCTGAAGTGGAAAGTGCACTGGTGCTGCATGACAGCGTGGCCGAAGCCGCGGTGGTCGGCTACCCCCACGACCTCAAGGGCCAAGGCGTCTACGCCTTCGTCACGACCATGAATGGCGTGACACCa                                                                                                                 <  1:1042548/150‑1 (MQ=255)
                                    gtCCGGGCACCGCATCGGCACCGCTGAAGTGGAAAGTGCACTGGTGCTGCATGACAGCGTGGCCGAAGCCGCGGTGGTCGGCTACCCCCACGACCTCAAGGGCCAAGGCGTCTACGCCTTCGTCACGACCATGAATGGCGTGACACCAGa                                                                                                               <  2:844302/150‑1 (MQ=255)
                                     tCCGGGCACCGCATCGGCACCGCTGAAGTGGAAAGTGCACTGGTGCTGCATGACAGCGTGGCCGAAGCCGCGGTGGTCGGCTACCCCCACGACCTCAAGGGCCAAGGCGTCTACGCCTTCGTCACGACCATGAATGGCGTGACACCAGAt                                                                                                              >  2:1181526/1‑150 (MQ=255)
                                            aCCGCATCGGCACCGCTGAAGTGGAAAGTGCACTGGTGCTGCATGACAGCGTGGCCGAAGCCGCGGTGGTCGGCTACCCCCACGACCTCAAGGGCCAAGGCGTCTACGCCTTCGTCACGACCATGAATGGCGTGACACCAGATGACACGc                                                                                                       >  1:961830/1‑150 (MQ=255)
                                               gCATCGGCACCGCTGAAGTGGAAAGTGCACTGGTGCTGCATGACAGCGTGGCCGAAGCCGCGGTGGTCGGCTACCCCCACGACCTCAAGGGCCAAGGCGTCTACGCCTTCGTCACGACCATGAATGGCGTGa                                                                                                                      >  2:904850/1‑132 (MQ=255)
                                               gCATCGGCACCGCTGAAGTGGAAAGTGCACTGGTGCTGCATGACAGCGTGGCCGAAGCCGCGGTGGTCGGCTACCCCCACGACCTCAAGGGCCAAGGCGTCTACGCCTTCGTCACGACCATGAATGGCGTGa                                                                                                                      <  1:904850/132‑1 (MQ=255)
                                                 aTCGGCACCGCTGAAGTGGAAAGTGCACTGGTGCTGCATGACAGCGTGGCCGAAGCCGCGGTGGTCGGCTACCCCCACGACCTCAAGGGCCAAGGCGTCTACGCCTTCGTCACGACCATGAATGGCGTGACACCAGATGACACGCTCAAg                                                                                                  <  2:926929/150‑1 (MQ=255)
                                                   cGGCACCGCTGAAGTGGAAAGTGCACTGGTGCTGCATGACAGCGTGGCCGAAGCCGCGGTGGTCGGCTACACCCACGACCTCAAGAGCCAAGGCGTGTAAGGAAACGGCGATACCAGGAGTGTCGGTACACGAGATGACACGCTAAAggg                                                                                                >  2:919149/1‑149 (MQ=255)
                                                    ggCACCGCTGAAGTGGAAAGTGCACTGGTGCTGCATGACAGCGTGGCCGAAGCCGCGGTGGTCGGCTACCCCCACGACCTCAAGGGCCAAGGCGTCTACGCCTTCGTCACGACCATGAATGGCGTGACACCAGATGACACGCTCAAGGcc                                                                                               >  1:809773/1‑150 (MQ=255)
                                                     gCACCGCTGAAGTGGAAAGTGCACTGGTGCTGCATGACAGCGTGGCCGAAGCCGCGGTGGTCGGCTACCCCCACGACCTCAAGGGCCAAGGCGTAGACGCCttgg                                                                                                                                           >  2:895905/1‑103 (MQ=255)
                                                     gCACCGCTGAAGTGGAAAGTGCACTGGTGCTGCATGACAGCGTGGCCGAAGCCGCGGTGGTCGGCTACCCCCACGACCTCAAGGGCCAAGGCGTAGACGCCttgg                                                                                                                                           <  1:895905/105‑3 (MQ=255)
                                                     gCACCGCTGAAGTGGAAAGTGCACTGGTGCTGAATGACAGCGTGGCCGAAGCCGCGGTGGTCGGCTACCCCCACGACCTCAAGGGCCAAGGCGTCTACGCCTTCGTCACGACCATGAATGGCGTGACACAAGATGACAAGCTCAAGGACg                                                                                              >  1:485187/1‑150 (MQ=255)
                                                       aCCGCTGAAGTGGAAAGTGCACTGGTGCTGCATGACAGCGTGGCCGAAGCCGCGGTGGTCGGCTACCCCCACGACCTCAAGGGCCAAGGCGTCTACGCCTTCGTCACGACCATGAATGGCGTGa                                                                                                                      <  1:285282/124‑1 (MQ=255)
                                                       aCCGCTGAAGTGGAAAGTGCACTGGTGCTGCATGACAGCGTGGCCGAAGCCGCGGTGGTCGGCTACCCCCACGACCTCAAGGGCCAAGGCGTCTACGCCTTCGTCACGACCATGAATGGCGTGa                                                                                                                      >  2:285282/1‑124 (MQ=255)
                                                            tGAAGTGGAAAGTGCACTGGTGCTGCATGACAGCGTGGCCGAAGCCGCGGTGGTCGGCTACCCCCACGACCTCACGACCAAAGGCGTCTACGCCACCGGTTGCTTGACCAGTGTGGTGGCACCAGATGACACGCTCAAGGCCGAgctgct                                                                                       <  1:812341/150‑1 (MQ=255)
                                                                 tGGAAAGTGCACTGGTGCTGCATGACAGCGTGGCCGAAGCCGCGGTGGTCGGCTACCCCCACGACCTCAAGGGCCAAGGCGTCTACGCCTTCGTCACGACCATGAATGGCGTGACACCAGATGACACGCTCAAGGCCGAGCTGCTGGccc                                                                                  <  1:1181526/150‑1 (MQ=255)
                                                                   gAAAGTGCACTGGTGCTGCATGACAGCGTGGCCGAAGCCGCGGTGGTCGGCTACCCCCACGACCTCAAGGGCCAAGGCGTCTACGCCTTCGTCACGACCATGAATGGCGTGACACCAGATGACACTCTCAAGGCCGAGCTGCTGGCCCTg                                                                                >  2:176285/1‑150 (MQ=255)
                                                                       gTGCACTGGTGCTGCATGACAGCGTGGCCGAAGCCGAGGTGACTGGCTACCCCCACGACCTCAAGGGCCAAGGCGTCTACGCCTTCGTCACGACCATGAATGGCGTGACACCAGATGACACGCTCAAGGCCGAGCTGCTGGCCCTGGTCa                                                                            <  1:919149/150‑1 (MQ=255)
                                                                           aCTGGGGCTGCATGACAGGGTGGCCGAAGCCGCGGTGGTCGGCTACCCTCACGACCTCAAGGGCCAAGGCGTCTACGCCTTCGTCACGACCATGAATGGCGTGACACCAGATGACACGCTCAAGGCCGAGCTGCTGGCCCTGGTCAGCaa                                                                        <  2:624362/150‑1 (MQ=255)
                                                                            cTGGTGCTGCATGACAGCGTGGCCGAAGCCGCGGTGGTCGGCTACCCCCACGACCTCAAGGGCCAAGGCGTCTACGCCTTCGTCACGACCATGAATGGCGTGACACCAGATGACACGCTCAAGGCCGAGCTGCTGGCCCTGGTCAGCAAg                                                                       <  1:176285/150‑1 (MQ=255)
                                                                               gTGCTGCATGACAGCGTGGCCGAAGCCGCGGTGGTCGGCTACCCCCACGACCTCAAGGGCCAAGGCGTCTACGCCTTCGTCACGACCATGAATGGCGTGACACCAGATGACACGCTCAAGGCCGAGCTGCTGGCCCTGGTCAGCAAGGaa                                                                    >  1:106100/1‑150 (MQ=255)
                                                                                  cttcATTACATCGTGGCCGAAGCCGCGGTGGTCGGCTACCCCCACGACCTCAAGGGCCAAGGCGTCTACGCCTTCGTCACGACCATGAATGGCGTGACACCAGATGACACGCTCAAGGCCGAGCTGCTGGCCCTGGTCAGCAAGGAAATc                                                                 <  2:485187/147‑1 (MQ=255)
                                                                                   tgcATGACAGCGTGGCCGAAGCCGCGGTGGTTGGCTACCCCCACGACCTCAAGGGCCAAGGCGTCTACGCCTTCGTCACGACCATGAATGGCGTGACACCAGATGACACGCTCAAGGCCGAGCTGCTGGCCCTGGTCAGCAAGGAAATCg                                                                <  1:607086/150‑1 (MQ=255)
                                                                                   tgcATGACAGCGTGGCCGAAGCCGCGGTGGTCGGCTACCCCCACGACCTCAAGGGCCAAGGCGTCTACGCCTTCGTCACGACCATGAATGGCGTGACACCAGATGACACGCTCAAGGCCGAGCTGCTGGCCCTGGTCAGCAAGGAAATCg                                                                <  2:961830/150‑1 (MQ=255)
                                                                                     cATGACAGCGTGGCCGAAGCCGCGGTGGTCGGCTACCCCCACGACCTCAAGGGCCAAGGCGTCTACGCCTTCGTCACGACCATGAATGGCGTGACACCAGATGACACGCTCAAGGCCGAGCTGCTGGCCCTGGTCAGCAAGGAAATCGGc                                                              >  1:14007/1‑150 (MQ=255)
                                                                                           aGCGTGGCCGAAGCCGCGGTGGTCGGCTACCCCCACGACCTCAAGGGCCAAGGCGTCTACGCCTTCGTCACGACCATGAATGGCGTGACACCAGATGa                                                                                                            <  2:166013/98‑1 (MQ=255)
                                                                                           aGCGTGGCCGAAGCCGCGGTGGTCGGCTACCCCCACGACCTCAAGGGCCAAGGCGTCTACGCCTTCGTCACGACCATGAATGGCGTGACACCAGATGa                                                                                                            >  1:166013/1‑98 (MQ=255)
                                                                                                  ccGAAGCCGCGGTGGTCGGCTACCCCCACGACCTCAAGGGCCAAGGCGTCTACGCCTTCGTCa                                                                                                                                        >  2:1131765/1‑63 (MQ=255)
                                                                                                  ccGAAGCCGCGGTGGTCGGCTACCCCCACGACCTCAAGGGCCAAGGCGTCTACGCCTTCGTCa                                                                                                                                        <  1:1131765/63‑1 (MQ=255)
                                                                                                   cGAAGCCGCGGTGGTCGGCTGCCCCCACGACCTCAAGGGCCAAGGCGTCTACGCCTTCGTCACGACCATGAATGGCGTGACACCAGATGACACGCTCAAGGCCGAGCTGCTGGCCCTGGt                                                                              <  1:624515/120‑1 (MQ=255)
                                                                                                   cGAAGCCGCGGTGGTCGGCTACCCCCACGACCTCAAGGGCCAAGGCGTCTACGCCTTCGTCACGACCATGAATGGCGTGACACCAGATGACACGCTCAAGGCCGAGCTGCTGGCCCTGGt                                                                              >  2:624515/1‑120 (MQ=255)
                                                                                                     aaGCCGCGGTGGTCGGCTACCCCCACGACCTCAAGGGCCAAGGCGTCTACGCCTTCGTCACGACCATGAATGGCGTGACACCAGATGACACGCTCAAGGCCGAGCTGCTGGCCCTGGTCAGCAAGGAAATCGGCAGCTTCGCCAAGCCTG                                              <  1:422342‑M1/150‑1 (MQ=255)
                                                                                                                gtCGGCTACCCCCACGACCTCAAGGGCCAAGGCGTCTACGCCTTCGTCACGACCATGAATGGCGTGa                                                                                                                      >  2:42433/1‑67 (MQ=255)
                                                                                                                gtCGGCTACCCCCACGACCTCAAGGGCCAAGGCGTCTACGCCTTCGTCACGACCATGAATGGCGTGa                                                                                                                      <  1:42433/67‑1 (MQ=255)
                                                                                                                     cTACCCCCACGACCTCAAGGGCCAAGGCGTCTACGCCTTCGTCACGACCATGAATGGCGTGACACCAGATGACACGCTCAAGGCCGAGCTGCTGGCCCTGGTCAGCAAGGAAATCGGCAGCTTCGCCAAGCCTGAGCTGATTCAGTGGgc                              <  2:809773/150‑1 (MQ=255)
                                                                                                                         ccccACGACCTCAAGGGCCAAGGCGTCTACGCCTTCGTCACGACCATGAATGGCGTGACACCAGATGACACGCTCAAGGCCGAGCTGCTGGCCCTGGTCAGCAAGGAAATCGGCAGCTTCGCCAAGCCTGAGCTGATTCAGTGGGCGCCa                          <  2:124383/150‑1 (MQ=255)
                                                                                                                           ccACGACCTCAAGGGCCAAGGCGTCTACGCCTTCGTCACGACCATGAATGGCGTGACACCAGATGACACGCTCAAGGCCGAGCTGCTGGCCCTGGTCAGCAAGGAAATCGGCAGCTTCGCCAAGCCTGAGCTGATTCAGTGGGCGCCAgc                        >  1:325394/1‑150 (MQ=255)
                                                                                                                                   tCAAGGGCCAAGGCGTCTACGCCTTCGTCACGACCATGAATGGCGTGACACCAGATGACACTCTCAAGGCCGAGCTGCCGGTCCTGGTCAGCAAGGAAATCGGCAGCTTCGCCAAGCCTGAGCTGACGCAGTGTGCGCCAGCGCTACCCa                >  1:327083/1‑150 (MQ=255)
                                                                                                                                        ggCCAAGGCGTCTACGCCTTCGTCATGACCATGAATGGCCTGACACCAGATGACACGCTCAAGGCCGAGCTGCTGGCCTTGGTCAGCAAGGAAATCGGCAGCTCCCCAAAGCCTGAGCTGATTCAGTGGGCGCAAGCGCTACCCAAGAcg           <  2:233430/150‑1 (MQ=255)
                                                                                                                                          cgaaggCGTAGACGCCTTCGTCAGGACCATGAATGGCGTGACACCAGATGACACGCTCAAGGCCGAGCTGCTGGCCCTGGTCAGCAAGGAAATCGGCAGCTTCGCCAAGACTGAGCTGATTCAGTGGGCGCCAGCGCTACCCAAGACGCg         <  2:72294‑M2/148‑2 (MQ=255)
                                                                                                                                                 gTCTACGCCTTCGTCACGACCATGAATGGCGTGACACCAGATGACACGCTCAAGGCCGAGCTGCTGGCCCTGGTCAGCAAGGAAATCGGCAGCTTCGCCAAGCCTGAGCTGATTCAGTGGGCGCCAGCGCTACCCAAGACGCgctcaggc  >  2:796046‑M2/1‑142 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
CTGGATCACCGGGCGCATCGACGATGTGATCAACGTGTCCGGGCACCGCATCGGCACCGCTGAAGTGGAAAGTGCACTGGTGCTGCATGACAGCGTGGCCGAAGCCGCGGTGGTCGGCTACCCCCACGACCTCAAGGGCCAAGGCGTCTACGCCTTCGTCACGACCATGAATGGCGTGACACCAGATGACACGCTCAAGGCCGAGCTGCTGGCCCTGGTCAGCAAGGAAATCGGCAGCTTCGCCAAGCCTGAGCTGATTCAGTGGGCGCCAGCGCTACCCAAGACGCGCTCAGGC  >  NC_002947/5346168‑5346462

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑