Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,427,336 T→A 10.4% D272V (GAC→GTC)  rhlE‑I ← ATP‑dependent RNA helicase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,427,3360TA10.4% 148.5 / 6.4 57D272V (GAC→GTC) rhlE‑IATP‑dependent RNA helicase
Reads supporting (aligned to +/- strand):  ref base T (23/26);  new base A (3/3);  total (28/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01

GCAGGTCGAGGTTGACGACCAGCGGCAAGTCATCGATATCCAGGCCACGGGCCGCCACATCGGTCGCCACCAGCACTTGCACCTCACGGGCCTTGAAGCTGTCCAGCGCACGCTGGCGGGTTGCCTGTGGGCGGTCACCGTGGATGCCGTCGGCATTCACGCCTTCGGCCAGCAAGCGCTCCACCAGCTGATCGACGCCATTGCGGGTCTTGGCGAACACCAGCACCTGCTTCCAGCGCTGCTTGCGCAGCAGGTGGCAGAACAGGTCAGCCTTGCGCTTCTTGTCCACAGG  >  NC_002947/5427187‑5427478
                                                                                                                                                     |                                                                                                                                              
gCAGGTCGAGGTTGACGACAAGCGGCAAGTCATCGATATCCAGGCCACGGGCCGCCACATCGGTCGCCACCAGCACTTGCACCTCACGGGCCTTGAAGCTGTCCAGCGCACGCTGGCGGGTTGCCTGTGGGCGGTCACCGTGGATGCCGt                                                                                                                                                >  1:1022039/1‑150 (MQ=255)
    gTCGAGGTTGACGACCAGCGGCAAGTCATCGATATCCAGGCCACGGGCCGCCACATCGGTCGCCACCAGCACTTGCACCTCACGGGCCTTGAAGCTGTCCAGCGCACGCTGGCGGGTTGCCTGTGGGCGGTCACCGTGGATGCCGTCGGc                                                                                                                                            <  1:986760/150‑1 (MQ=255)
            tGACGACCAGCGGCAAGTCATCGATATCCAGGCCACGGGCCGCCACATCGGTCGCCACAAGAACTTCCACCTCACGGGCCTTGAAGCTGTCCAGCGCACGCTGGCGGGTTGCCTGTGGGCGGTCACCGTGGATGCCGTCGGCATTCACGc                                                                                                                                    <  2:891509/150‑1 (MQ=255)
                gacCAGCGGCAAGTCATCGATATCCAGGCCACGGGCCGCCACATCGGTCGCCACCAGCACTTGCACCTCACGGGCCTTGAAGCTGTCCAGCGCACGCTGGCGGGTTGCCTGTGGGCGGTCACCGTGGATGCCGTCGGCATTCACGCCTTc                                                                                                                                <  1:893679/150‑1 (MQ=255)
                          aaGTCATCGATATCCAGGCCACGGGCCGCCACATCGGTCGCCACCAGCACTTGCACCTCACGGGCCTTGAAGCTGTCCAGCGCACGCTGGCGGGTTGCCTGTGGGCGGTCACCGTGAATGCCGACGGCATCCACg                                                                                                                                     <  1:84866/135‑1 (MQ=255)
                          aaGTCATCGATATCCAGGCCACGGGCCGCCACATCGGTCGCCACCAGCACTTGCACCTCACGGGCCTTGAAGCTGTCCAGCGCACGCTGGCGGGTTGCCTGTGGGCGGTCACCGTGAATGCCGACGGCATCCACg                                                                                                                                     >  2:84866/1‑135 (MQ=255)
                             tCATCGATATCCAGGCCACGGGCCGCCACATCGGTCGCCACCAGCACTTGCACCTCACGGGCCTTGAAGCTGTCCAGCGCACGCTGGCGGGTTGCCTGTGGGCGGTCACCGTGGATGCCGTCGGCATTCACGCCTTCGGCCAGCAAgcgc                                                                                                                   >  2:894269/1‑150 (MQ=255)
                             tCATCGATATCCAGGCCACGGGCCGCCACATCGGTCGCCACCAGCACTTGCACCTCACGGGCCTTGAAGCTGTCCAGCGCACGCTGGCGGGTTGCCTGTGGGCGGTCACCGTGGATGCCGTCGGCATTCACGCCTTCGGCCAGCAAgcgc                                                                                                                   <  1:501014/150‑1 (MQ=255)
                               aTCGATATCCAGGCCACGGGCCGCCACATCGGTCGCCACCAGCACTTGCACCTCACGGGCCTTGAAGCTGTCCAGCGCACGCTGGCGGGTTGCCTGTGGGCGGTCACCGTGGATGCCGTCGGCATTCACGCCTTc                                                                                                                                >  1:1002498/1‑135 (MQ=255)
                               aTCGATATCCAGGCCACGGGCCGCCACATCGGTCGCCACCAGCACTTGCACCTCACGGGCCTTGAAGCTGTCCAGCGCACGCTGGCGGGTTGCCTGTGGGCGGTCACCGTGGATGCCGTCGGCATTCACGCCTTc                                                                                                                                <  2:1002498/135‑1 (MQ=255)
                                tCGATATCCAGGCCACGGGCCGCCACATCGGTCGCCACCAGCACTTGCACCTCACGGGCCTTGAAGCTGTCCAGCGCACGCTGGCGGGTTGCCTGTGGGCGGTCACCGTGAATGCCGACGGCAtc                                                                                                                                         >  2:299169/1‑124 (MQ=255)
                                tCGATATCCAGGCCACGGGCCGCCACATCGGTCGCCACCAGCACTTGCACCTCACGGGCCTTGAAGCTGTCCAGCGCACGCTGGCGGGTTGCCTGTGGGCGGTCACCGTGAATGCCGACGGCAtc                                                                                                                                         <  1:299169/125‑2 (MQ=255)
                                     atCCAGGCCACGGGCCGCCACATCGGTCGCCACCAGCACTTGCACCTCACGGGCCTTGAAGCTGTCCAGCGCACGCTGGCGGGTTGCCTGTGGGCGGTCACCGTGGATGCCGTCGGCATTCACGCCTTCGGCCAGCAAGCGCTCCACCAg                                                                                                           >  1:334137/1‑150 (MQ=255)
                                      tCCAGGCCACGGGCCGCCACATCGGTCGCCACCAGCACTTGCACCTCACGGGCCTTGAAGCTGTCCAGCGCACGCTGGCGGGTTGCCTGTGGGCGGTCACCGTGGATGCCGTCGGCATTCACGCCTTCGGCCAGCAAGCGCTCCACCAGc                                                                                                          <  1:596000/150‑1 (MQ=255)
                                       ccAGGCCACGGGCCGCCACGTCGGTCGCCACGAGCACTTCCAGCTCACGGGCCTTGAAGCTGTCCAGCGCACGCTGGCGGGTTGCCTGTGGGCGGTCACCGTGGATGCCGTCGGCATTCCCGCCTTCGGCCAGCAAGCGCTCCACCAGCt                                                                                                         >  2:1043726/1‑150 (MQ=255)
                                        cAGGCCACGGGCCGCCACATCGGTCGCCACCAGCACTTGCACCTCACGGGCCTTGAAGCTGTCCAGCGCACGCTGGCGGGTTGCCTGTGGGCGGTCACCGTGGATGCAGGCGGCATTCACGCCTTCGGCCAGCAAGCGCTCCACCAGCTg                                                                                                        >  1:11519/1‑150 (MQ=255)
                                                        aaagcGGTCGCGACAAGAACTTGCGCGGCACGGGCGATGAGGCTGGCCAGCGCACGCTGGCGGGGTGCCTGTGGACGATGACCGTGGAGGCCGTCGGCATTCACACCTTCGGCCAGCAAGCCCTCCACCAGCTGATCGACGCCATGGCgg                                                                                        >  2:81143/5‑150 (MQ=255)
                                                              gTCGCCACCAGCACTTGCACCTCACGGGCCTTGAAGCTGTCCAGCGCACGCTGGCGGGTTGCCTGTGGGCGGTCACCGTGGATGCCGTCGGCATTCACGCCTTCGGCCAGCAAGCGCTCCACCAGCTGATCGACGCCATTGCGGGTCTTg                                                                                  <  2:1022039/150‑1 (MQ=255)
                                                               tCGCCACCAGCACTTGCACCTCACGGGCCTTGAAGCTGTCCAGCGCACGCTGGCGGGTTGCCTGTGGGCGGTCACCGTGGATGCCGTCGGCATTCACGCCTTCGGCCAGCAAGCGCTCCACCAGCTGATCGACGCCATTGCGGGTCTTgg                                                                                 <  1:890134/150‑1 (MQ=255)
                                                                  ccaccaGCACTTGCACCTCACGGGCCTTGAAGCTGTCCAGCGCACGCTGGCGGGTTGCCTGTGGGCGGTCACCGTGGATGCCGTCGGCATTCACGCCTTCGGCCAGCAAGCGCTCCACCAGCTGATCGACGCCATTGCGGGTCTTGGCGa                                                                              >  1:99524/1‑150 (MQ=255)
                                                                    accaGCACTTGCACCTCACGGGCCTTGAAGCTGTCCAGCGCACGCTGGCGGGTTGCCTGTGGGCGGTCACCGTGGATGCCGTCGGCATTCACGCCTTCGGCCAGCAAGCGCTCCACCAGCTGATCGACGCCATTGCGGGTCTTg                                                                                  <  1:812921/144‑1 (MQ=255)
                                                                    accaGCACTTGCACCTCACGGGCCTTGAAGCTGTCCAGCGCACGCTGGCGGGTTGCCTGTGGGCGGTCACCGTGGATGCCGTCGGCATTCACGCCTTCGGCCAGCAAGCGCTCCACCAGCTGATCGACGCCATTGCGGGTCTTg                                                                                  >  2:812921/1‑144 (MQ=255)
                                                                     ccaGCACTTGCACCTCACGGGCCTTGAAGCTGTCCAGCGCACGCTGGCGGGTTGCCTGTGGGCGGTCACCGTGGATGCCGTCGGCATTCACGCCTTCGGCCAGCAAGCGCTCCACCAGCTGATCGACGCCATTGCGGGTCTTGGCGAaca                                                                           >  2:213193/1‑150 (MQ=255)
                                                                               cACCTCACGGGCCTTGAAGCTGTCCAGCGCACGCTGGCGGGTTGCCTGTGGGCGGTCACCGTGGATGCCGTCGGCATTCACGCCTTCGGCCAGCAAGCGCTCCACCAGCTGATCGACGCCATTGCGGGTCTTGGCGAACACCAGCACCTg                                                                 <  2:334137/150‑1 (MQ=255)
                                                                                aCCTCACGGGCCTTGAAGCTGTCCAGCGCACGCTGGCGGGTTGCCTGTGGGCGGTCACCGTGGATGCCGTCGGCATTCACGCCTTCGGCCAGCAAGCGCTCCACCAGCTGATCGACGCCATTGCGGGTCTTGGCGAACACCAGCACCTGc                                                                <  2:501657/150‑1 (MQ=255)
                                                                                       gggCCTTGAAGCTGTCCAGCGCACGCTGGCGGGTTGCCTGTGGGCGGTCACCGTGGATGCCGTCGGCATTCACGCCTTCGGCCAGCAAGCGCTCCACCAGCTGATCGACGCCATTGCGGGTCTTGGCGAACACCAGCACCTGCTTCCAgc                                                         >  2:3471/1‑150 (MQ=255)
                                                                                               aaGCTGTCCAGCGCACGCTGTCGGGTTGCCTGTGGGCGGTCACCGTGGATGCCGTCGGCATTCACGCCTTCGGCCAGCAAGCGCTCCACCAGCTGATCGACGCCATTGCGGGTCTTGGCGAACACCAGCACCTGCTTCCAGCGCTGCTTg                                                 >  2:428861/1‑150 (MQ=255)
                                                                                                   tGTCCAGCGCACGCTGGCGGGTTGCCTGTGGGCGGTCACCGTGGATGCCGTCGGCATTCACGCCTTCGGCCAGCAAGCGCTCCACCAGCTGATCGACGCCATTGCCGGTCTTGGCGAACACCAGCACCTGCTTCCAGCGCTGCTTGCgca                                             <  2:456650/150‑1 (MQ=255)
                                                                                                    gTCCAGCGCACGCTGGCGGGTTGCCTGTGGGCGGTCACCGTGGATGCCGTCGGCATTCACGCCTTCGGCCAGCAAGCGCTCCACCAGCTGATCGACGCCATTGCGGGTCTTg                                                                                  <  2:814521/112‑1 (MQ=255)
                                                                                                    gTCCAGCGCACGCTGGCGGGTTGCCTGTGGGCGGTCACCGTGGATGCCGTCGGCATTCACGCCTTCGGCCAGCAAGCGCTCCACCAGCTGATCGACGCCATTGCGGGTCTTg                                                                                  >  1:814521/1‑112 (MQ=255)
                                                                                                       cAGCGCACGCTGGCGGGTTGCCTGTGGGCGGTCACCGTGGATGCCGTCGGCATTCACGCCTTCGGCCAGCAAGCGCTCCACCAGCTGATCGACGCCATTGCGGGTCTTGGCGAACACCAGCACCTGCTTCCAGCGCTGCTTGCGcagcag                                         >  1:264072/1‑150 (MQ=255)
                                                                                                          cgcACGCTGTCGGGTTGCCTGTGGGCGGTCACCGTGGATGCCGTCGGCATTCACGCCTTCGGCCAGCAAGCGCTCCACCAGCTGATCGACGCCATTGCGGGTCTTGGCGAACACCAGCACCTGCTTCCAGCGCTGCTTGCGCAGCAGGTg                                      <  1:428861/150‑1 (MQ=255)
                                                                                                                   gCGGGTTGCCTGTGGGCGGTCACCGTGGATGCCGTCGGCATTCACGCCTTCGGCCAGCAAGCGCTCCACCAGCTGATCGACGCCATTGCGGGTCTTGGCGAACACCAGCACCTGCTTCCAGCGCTGCTTGCGCAGCAGGTGGCAGAACAg                             >  1:315090/1‑150 (MQ=255)
                                                                                                                     gggTTGCCTGTGGGCGGTCACCGTGGATGCCGTCGGCATTCACGCTTTCGGCCAGCAAGCGCTCCACCAGCTGATCGATGACCGTGCGCGTGTTGAGGAACACGAGGACCACATTACAGAGCTCCTTTCCCAGCAGGTGGCAGAACAggg                           >  1:928177/1‑149 (MQ=255)
                                                                                                                     gggTTGCCTGTGGGCGGTCACCGTGGATGCCGTCGGCATTCACGCCTTCGGCCAGCAAGCGCTCCACCAGCTGATCGACGCCATTGCGGGTCTTGGCGAACACCAGCACCTGCTTCCAGCGCTGCTTGCGCAGCAGGTGGCAGAACAGGt                           >  2:433607/1‑150 (MQ=255)
                                                                                                                     gggTTGCCTGTGGGCGGTCACCGTGGATGCCGTCGGCATTCACGCCTTCGGCCAGCAAGCGCTCCACCAGCTGATCGACGCCATTGCGGGTCTTGGCGAACACCAGCACCTGCTTCCAGCGCTGCTTGCGCAGCAGGTGGCAGAACAGGt                           >  2:235336/1‑150 (MQ=255)
                                                                                                                       gTTGCCTGTGGGCGGTCACCGTGGATGCCGTCGGCATTCACGCCTTCGGCCAGCAAGCGCTCCACCAGCTGATCGACGCCATTGCGGGTCTTGGCGAACACCAGCACCTGCTTCCAGCGCTGCTTGCGCAGCAGGTGGCAGAACAGGTCa                         <  2:99524/150‑1 (MQ=255)
                                                                                                                             tgtgGGCGGTCACCGTGGATGCCGTCGGCATTCACGCCTTCGGCCAGCAAGCGCTCCACCAGCTGATCGACGCCATTGCGGGTCTTGGCGAACACCAGCACCTGCTTCCAGCGCTGCTTgc                                                >  1:349486/1‑121 (MQ=255)
                                                                                                                             tgtgGGCGGTCACCGTGGATGCCGTCGGCATTCACGCCTTCGGCCAGCAAGCGCTCCACCAGCTGATCGACGCCATTGCGGGTCTTGGCGAACACCAGCACCTGCTTCCAGCGCTGCTTgc                                                <  2:349486/121‑1 (MQ=255)
                                                                                                                             tgtgGGCGGTCACCGTGGATGCCGTCGGCATTCACGCCTTCGGCCAGCAAGCGCTCCACCAGCTGATCGACGCCATTGCGGGTCTTGGCGAACACCAGCACCTGCTTCCAGCGCTGCTTGCGCAGCAGGTGGCAGAACAGGTCAGCCTTg                   <  1:213193/150‑1 (MQ=255)
                                                                                                                             tgtgGGCGGTCACCGTGGATGCCGTCGGCATTCACGCCTTCGGCCAGCAAGCGCTCCACCAGCTGATCGACGCCATTGCGGGTCTTGGCGAACACCAGCACCTGCTTCCAGCGCTGCTTGCGCAGCAGGTGGCAGAACAGGTCAGCCTTg                   >  2:1117449/1‑150 (MQ=255)
                                                                                                                             tgtgGGCGGTCACCGTGGATGCCGTCGGCATTCACGCCTTCGGCCAGCAAGCGCTCCACCAGCTGATCGACGCCATTGCGGGTCTTGGCGAACACCAGAACCTGCTTCCAGCGCTGCTTGCGCAGCAGGTGGCAGAACAGGTCAGCCTTg                   <  1:894269/150‑1 (MQ=255)
                                                                                                                                gggCGGTCACCGTGGATGCCGTCGGCATTCACGCCTTCGGCCAGCAAGCGCTCCACCAGCTGATCGACGCCATTGCGGGTCTTGGCGAACACCAGCACCTGCTTCCAGCGCTGCTTGCGCAGCAGGTGGCAGAACAGGTCAGCCTTgcgc                <  1:674642/150‑1 (MQ=255)
                                                                                                                                      tCACCGTGGATACCAGCGGCATTTACGCCTTCGGCCAGCAGGCGCTCCACCAGCTGATCGACGCCATTGCGGGTCTTGGCGAACACCAGCACCTGCTTCAAGAGATCCTTACGACGAAGCCCGGAGAACAGGCCATCCTGGCGCTTCTTg          >  2:734471/1‑150 (MQ=255)
                                                                                                                                        aCCGTGGATGCCGTCGGCATTCACGCCTTCGGCCAGCAAGCGCTCCACCAGCTGATCGACGCCATTGCGGGTCTTGGCGAACACCAGCACCTGCTTCCAGCGCTGCTTGCGCAGCAGGTGGCAGAACAGGTCAGCCTTGCGCTTCTTGTc        <  2:315090/150‑1 (MQ=255)
                                                                                                                                         ccGTGGATGCCGTCGGCATTCACGCCTTCGGCCAGCAAGCGCTCCACCAGCTGATCGACGCCATTGCGGGTCTTGGCGAACACCAGCACCTGCTTCCAGCGCTGCTTGCGCAGCAGGTGGCAGAACAGGTCAGCCTTGCGCTTCTTGTcc       >  2:194780/1‑150 (MQ=255)
                                                                                                                                          cGTGGATGCCGTCGGCATTCACGCCTTCGGCCAGCAAGCGCTCCACCAGCTGATCGACGCCATTGCGGGTCTTGGCGAACACCAGCACCTGCTTCCAg                                                          >  2:1054725/1‑98 (MQ=255)
                                                                                                                                          cGTGGATGCCGTCGGCATTCACGCCTTCGGCCAGCAAGCGCTCCACCAGCTGATCGACGCCATTGCGGGTCTTGGCGAACACCAGCACCTGCTTCCAg                                                          <  1:1054725/98‑1 (MQ=255)
                                                                                                                                          cGTGGATGCCGTCGGCATTCACGCCTTCGGCCAGCAAGCGCTCCACCAGCTGATCGACGCCATTGCGGGTCTTGGCGAACACCAGCACCTGCTTCCAGCGCTGCTTGCGCAGCAGGTGGCAGAACAGGTCAGCCTTGCGCTTCTTGTCca      <  2:535180/150‑1 (MQ=255)
                                                                                                                                           gTGGATGCCGTCGGCATTCACGCCTTCGGCCAGCAAGCGCTCCACCAGCTGATCGACGCCATTGCGGGTCTTGGCGAACACCAGCACCTGCTTCCAGCGCTGCTTgc                                                >  2:148554/1‑107 (MQ=255)
                                                                                                                                           gTGGATGCCGTCGGCATTCACGCCTTCGGCCAGCAAGCGCTCCACCAGCTGATCGACGCCATTGCGGGTCTTGGCGAACACCAGCACCTGCTTCCAGCGCTGCTTgc                                                <  1:148554/107‑1 (MQ=255)
                                                                                                                                           gTGAATGCCGACGGCATCCACGCCTTCGGCCAGCAAGCGCTCCACCAGCTGATCGACGCCATTGCGGGTCTTGGCGAACACCAGCACCTGCTTCCAGCGCTGCTTGCGCAGCAGGTGGCAGAACAGGTCAGCCTTGCGCTTCTTGTCcac     >  1:906338/1‑150 (MQ=255)
                                                                                                                                           gTGAATGCCGACGGCATCCACGCCTTCGGCCAGCAAGCGCTCCACCAGCTGATCGACGCCATTGCGGGTCTTGGCGAACACCAGCACCTGCTTCCAGCGCTGCTTGCGCAGCAGGTGGCAGAACAGGTCAGCCTTGCGCTTCTTGTCcac     <  2:906338/150‑1 (MQ=255)
                                                                                                                                            tGGATGCCGTCGGCATTCACGCCTTCGGCCAGCAAGCGCTCCACCAGCTGATCGACGCCATTGCGGGTCTTGGCGAACACCAGCACCTGCTTCCAGCGCTGCTTGCGCAGCAGGTGGCAGAACAGGTCAGCCTTGCGCTTCTTGTCcaca    <  1:3471/150‑1 (MQ=255)
                                                                                                                                             ggATGCCGTCGGCATTCACGCCTTCGGCCAGCAAGCGCTCCACCAGCTGATCGACGCCATTGCGGGTCTTGGCGAACACCAGCACCTGCTTCCAGCGCTGCTTGCGCAGCAGGTGGCAGAACAGGTCAGCCTTGCGCTTCTTGTCCACAg   <  1:1043726/150‑1 (MQ=255)
                                                                                                                                              gATGCCGTCGGCATTCACGCCTTCGGCCAGCAAGCGCTCCACCAGCTGATCGACGCCATTGCGGGTCTTGGCGAACACCAGCACCTGCTTCCAGCGCTGCTTGCGCAGCAGGTGGCAGAACAGGTCAGCCTTGCGCTTCTTGTCCACAgg  >  2:447776/1‑150 (MQ=255)
                                                                                                                                                   cGTCGGCATTCACGCCTTCGGCCAGCAAGCGCTCCACCAGCTGATCGACGCCATTGCGGGTCTTGGCGAACACCAGCACCTGCTTCCAGCGCTGCTTGCGCAGCAGGTGGCAGAACAGGTCAGCCTTGCGCTTCTTGTCCACAg   >  2:1109089/1‑144 (MQ=255)
                                                                                                                                                   cGTCGGCATTCACGCCTTCGGCCAGCAAGCGCTCCACCAGCTGATCGACGCCATTGCGGGTCTTGGCGAACACCAGCACCTGCTTCCAGCGCTGCTTGCGCAGCAGGTGGCAGAACAGGTCAGCCTTGCGCTTCTTGTCCACAg   <  1:1109089/144‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                              
GCAGGTCGAGGTTGACGACCAGCGGCAAGTCATCGATATCCAGGCCACGGGCCGCCACATCGGTCGCCACCAGCACTTGCACCTCACGGGCCTTGAAGCTGTCCAGCGCACGCTGGCGGGTTGCCTGTGGGCGGTCACCGTGGATGCCGTCGGCATTCACGCCTTCGGCCAGCAAGCGCTCCACCAGCTGATCGACGCCATTGCGGGTCTTGGCGAACACCAGCACCTGCTTCCAGCGCTGCTTGCGCAGCAGGTGGCAGAACAGGTCAGCCTTGCGCTTCTTGTCCACAGG  >  NC_002947/5427187‑5427478

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑