Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,525,818 C→A 9.6% intergenic (+60/‑63) PP_4858 → / → PP_4859 hypothetical protein/TetR family transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,525,8180CA 9.6% 158.6 / 5.7 52intergenic (+60/‑63)PP_4858/PP_4859hypothetical protein/TetR family transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base C (22/25);  new base A (3/2);  total (25/27)
Fisher's exact test for biased strand distribution p-value = 6.62e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.70e-01

CGGCTCCGATCGTCTGATCGAACAAGCTGGCCGTGTCGCTGAAGGTGGCTCGGATCGCCTGGTTGAACTCAGCCGTGTGAGCTGATCGCCATGGCCGAAAAGAACAACTTCTGTCACAACGCTTGCTCCCCTCCAGGCCCGGTCCATTGACCGGGCTTTGTTTTTTTATCTAGAGTGCCCAGCCTGACCGTTACAGAAGCCCGCATCATGCTGCCGCGCGCCGAACAGAAGCTACAGACCCGCCAGGCCCTGCTCGATGCCGCCTGCCTGCTCATGGAGAGTGGCCGTGGTTT  >  NC_002947/5525674‑5525966
                                                                                                                                                |                                                                                                                                                    
cggcTCCGATCGTCTGATCGAACAAGCTGGCCGTGTCGCTGAAGGTGGCTCGGATCGCCTGGTTGAACTCAGCCGTGTGAGCTGATCGCCATGGCCGAAAAGAACAACTTCTGTCACAACGCTTGCTCCCCTCCAGGCCCGGTCCATTGa                                                                                                                                                 <  1:943983/150‑1 (MQ=255)
    tCCGATCGTCTGATCGAACAAGCTGGCCGTGTCGCTGAAGGTGGCTCGGATCGCCTGGTTGAACTCAGCCGTGTGAGCTGATCGCCATGGCCGAAAAGAACAACTTCTGTCACAACGCTTGCTCCCCTCCAGGCCCGGTCAATGGACCgg                                                                                                                                             <  2:625057/150‑1 (MQ=255)
      cGATCGTCTGATCGAACAAGCTGGCCGTGTCGCTGAAGGTGGCTCGGATCGCCTGGTTGAACTCAGCCGTGTGAGCTGATCGCCATGGCCGAAAAGAACAACTTCTGTCACAACGCTTCAGGCCCTCCAGGCCCGGTCAATGGACCGGGc                                                                                                                                           <  1:32027/150‑1 (MQ=255)
       gATCGTCTGATCGAACAAGCTGGCCGTGTCGCTGAAGGTGGCTCGGATCGCCTGGTTGAACTCAGCCGTGTGAGCTGATCGCCATGGCCGAAAAGAACAACTTCTGTCACAACGCTTGCTCCCCTCCAAGCCCGGTCAATGGACCGGGcc                                                                                                                                          >  2:1068691/1‑149 (MQ=255)
        aTCGTCTGATCGAACAAGCTGGCCGTGTCGCTGAAGGTGGCTCGGATCGCCTGGTTGAACTCAGCCGTGTGAGCTGATCGCCATGGCCGAAAAGAACAACTTCTGTCACAACGCTTGCTCCCCTCCAGGCCCGGTCCATTGACCGGGCtt                                                                                                                                         >  1:376264/1‑150 (MQ=255)
            tCTGATCGAACAAGCTGGCCGTGTCGCTGAAGGTGGCTCGGATCGCCTGGTTGAACTCAGCCGTGTGAGCTGATCGCCATGGCCGAAAAGAACAACTTCTGTCACAACGCTTGCTCCCCTCCAGGCCCGGTCCATTGACCGGGCTTTGtt                                                                                                                                     >  1:612679/1‑150 (MQ=255)
              tGATCGAACAAGCTGGCCGTGTCGCTGAAGGTGGCTCGGATCGCCTGGTTGAACTCAGCCGTGTGAGCTGATCGACATGGCCGAAAAGAACAACTTCTGTAACAACGCTTGCTCCCCTCCAGGCCCGGTCCATTGACCGGGCTTTGtttt                                                                                                                                   <  2:1043346/150‑1 (MQ=255)
               gATCGAACAAGCTGGCCGTGTCGCTGAAGGTGGCTCGGATCGCCTGGTTGAACTCAGCCGTGTGAGCTGATCGCCATGGCCGAAAAGAACAACTTCTGTCACAACGCTTGCTCCCCTCCAGGCCCGGTCCATTGACCGGGCTTTGttttt                                                                                                                                  <  2:586362/150‑1 (MQ=255)
               gATCGAACAAGCTGGCCGTGTCGCTGAAGGTGGCTCGGATCGCCTGGTTGAACTCAGCCGTGTGAGCTGATCGCCATGGCCGAAAAGAACAACTTCTGTCACAACGCTTGCTCCCCTCCAGGCCCGGTCCATTGACCGGGCTTTGttttt                                                                                                                                  <  1:252469/150‑1 (MQ=255)
                aTCGAACAAGCTGGCCGTGTCGCTGAAGGTGGCTCGGATCGCCTGGTTGAACTCAGCCGTGTGAGCTGATCGCCATGGCCGAAAAGAACAACTTCTGTCACAACGCTTGCTCCCCTCCAGGCCCGGTCCATTGACCGGGCTTTGtttttt                                                                                                                                 <  1:414942/150‑1 (MQ=255)
                             gCCGTGTCGCTGAAGGTGGCTCGGATCGCCTGGTTGAACTCAGCCGTGTGAGCTGATCGCCATGGCCGAAAAGAACAACTTCTGTCACAACGCTTGCTCCCCTCCAGGCCCGGTCCATTGACCGGGCTTTGTTTTTTTATCTAGAGTGcc                                                                                                                    <  1:1130552/150‑1 (MQ=255)
                             gCCGTGTCGCTGAAGGTGGCTCGGATCGCCTGGTTGAACTCAGCCGTGTGAGCTGATCGCCATGGCCGAAAAGAACAACTTCTGTCACAACGCTTGCTCCCCTCCAGGCCCGGTCCATTGACCGGGCTTTGTTTTTTTATCTAGAGTGcc                                                                                                                    >  1:591174/1‑150 (MQ=255)
                               cGTGTCGCTGAAGGTGGCTCGGATCGCCTGGTTGAACTCAGCCGTGTGAGCTGATCGCCATGGCCGAAAAGAACAACTTCTGTCACAACGCTTGCTCCCCTCCAGGCCCGGTCCATTGACCGGGCTTTGTTTTTTTATCTAGAGTTCCCa                                                                                                                  >  2:391906/1‑150 (MQ=255)
                                         aaGGTGGCTCGGATCGCCTGGTTGAACTCAGCCGTGTGAGCTGATCGCCATGGCCGAAAAGAACAACTTCTGTCACAACGCTTGCTCCCCTCCAGGCCCGGTCCATTGACCGGGCTTTGTTTTTTTATCTAGAGTGCCCAGCCTGACCGt                                                                                                        <  2:376264/150‑1 (MQ=255)
                                                         ccTGGTTGAACTCAGCCGTGTGAGCTGATCGCCATGGCCGAAAAGAACAACTTCTGTCACAACGCTTGCTCCCCTCCAGGCCCGGTCCATTGACCGGGCTTTGTTTTTTTATCTa                                                                                                                           >  2:357698/1‑115 (MQ=255)
                                                         ccTGGTTGAACTCAGCCGTGTGAGCTGATCGCCATGGCCGAAAAGAACAACTTCTGTCACAACGCTTGCTCCCCTCCAGGCCCGGTCCATTGACCGGGCTTTGTTTTTTTATCTa                                                                                                                           <  1:357698/115‑1 (MQ=255)
                                                         ccTGGTTGAACTCAGCCGTGTGAGCTGATCGCCATGGCCGAAAAGAACAACTTCTGTCACAACGCTTGCTCCCCTCCAGGCCCGGTCCATTGACCGGGCTTTGTTTTTTTATCTAGAGTGCCCAGCCTGACCGTTACAGAAGCCCGcatc                                                                                        >  1:1146583/1‑150 (MQ=255)
                                                            ggTTGAACTCAGCCGTGTGAGCTGATCGCCATGGCCGAAAAGAACAACTTCTGTCACAACGCTTGCTCCCCTCCAGGCCCGGTCCATTGACCGGGCTTTGTTTTTTTATCTAGAGTGCCCAGCCTGACCGTTACAGAAGCCCGCATCAtg                                                                                     >  2:386942/1‑150 (MQ=255)
                                                            ggTTGAACTCAGCCGTGTGAGCTGATCGCCATGGCCGAAAAGAACAACTTCTGTCACAACGCTTGCTCCCCTCCAGGCCCGGTCCATTGACCGGGCTTTGTTTTTTTATCTAGAGTGCCCAGCCTGACCGTTACAGAAGCCCGCATCAtg                                                                                     <  2:612679/150‑1 (MQ=255)
                                                             gTTGAACTCAGCCGTGTGAGCTGATCGCCATGGCCGAAAAGAACAACTTCTGTCACAACGCTTGCTCCCCTCCAGGCCCGGTCCATTGACCGGGCTTTGTTTTTTTATCTAGAGTGCCCAGCCTGACCGTTACAGAAGCCCGCATCAtgc                                                                                    >  1:1198086/1‑150 (MQ=255)
                                                              ttGAACTCAGCCGTGTGAGCTGATCGCCATGGCCGAAAAGAACAACTTCTGTCACAACGCTTGCTCCCCTCCAGGCCCGGTCCATTGACCGGGCTTTGTTTTTTTATCTAGAGTg                                                                                                                      <  2:1084933/115‑1 (MQ=255)
                                                              ttGAACTCAGCCGTGTGAGCTGATCGCCATGGCCGAAAAGAACAACTTCTGTCACAACGCTTGCTCCCCTCCAGGCCCGGTCCATTGACCGGGCTTTGTTTTTTTATCTAGAGTg                                                                                                                      >  1:1084933/1‑115 (MQ=255)
                                                               tGAACTCAGCCGTGTGAGCTGATCGCCATGGCCGAAAAGAACAACTTCTGTCACAACGCTTGCTCCCCTCCAGGCCCGGTCCATTGACCGGGCTTTGTTTTTTTATCTAGAGTGCCCAGCCTGACCGTTACAGAAGCCCGCATCAtgctg                                                                                  <  2:333286/150‑1 (MQ=255)
                                                                 aaCTCAGCCGTGTGAGCTGATCGCCATGGCCGAAAAGAACAACTTCTGTCACAACGCTTGCTCCCCTCCAGGCCCGGTCCATTGACCGGGCTTTGTTTTTTTATCTAGAGTGCCCAGCCTGACCGTTACAGAAGc                                                                                               >  1:767158/1‑135 (MQ=255)
                                                                 aaCTCAGCCGTGTGAGCTGATCGCCATGGCCGAAAAGAACAACTTCTGTCACAACGCTTGCTCCCCTCCAGGCCCGGGCCATTGACCGGGCTTTGTTTTTTTATCTAGAGTGCCCAGCCTGACCGTTACAGAAGc                                                                                               <  2:767158/135‑1 (MQ=255)
                                                                        ccGTGTGAGCTGATCGCCATGGCCGAAAAGAACAACTTCTGTCACAACGCTTGCTCCCCTCCAGGCCCGGTCCATTGACCGGGCTTTGTTTTTTTATCTAGAGTGCCCAGCCTGACCGTTACAGAAGCCCGCATCATGCTGCCGCGCGcc                                                                         >  1:673457/1‑150 (MQ=255)
                                                                          gtgtGAGCTGATCGCCATGGCCGAAAAGAACAACTTCTGTCACAACGCTTGCTCCCCTCCAGGCCCGGTCCATTGACCGGGCTTTGTTTTTTTATCTAGAGTGCCCAGCCTGACCGTTACAGAAGCCCGCATCAtgct                                                                                   >  2:425401/1‑138 (MQ=255)
                                                                          gtgtGAGCTGATCGCCATGGCCGAAAAGAACAACTTCTGTCACAACGCTTGCTCCCCTCCAGGCCCGGTCCATTGACCGGGCTTTGTTTTTTTATCTAGAGTGCCCAGCCTGACCGTTACAGAAGCCCGCATCAtgct                                                                                   <  1:425401/138‑1 (MQ=255)
                                                                           tgtgAGCTGATCGCCATGGCCGAAAAGAACAACTTCTGTCACAACGCTTGCTCCCCTCCAGGCCCGGTCCATTGACCGGGCTTTGTTTTTTTATCTAGAGTGCCCAGCCTGACCGTTACAGAAGCCCGCATCATGCTGCCGCGCGCCGaa                                                                      <  1:386942/150‑1 (MQ=255)
                                                                           tgtgAGCTGATCGCCATGGCCGAAAAGAACAACTTCTGTCACAACGCTTGCTCCCCTCCAGGCCCGGTCCATTGACCGGGCTTTGTTTTTTTATCTAGAGTGCCCAGCCTGACCGTTACAGAAGCCCGCATCATGCTGCCGCGCGCCGaa                                                                      <  1:383354/150‑1 (MQ=255)
                                                                                               cGAAAAGAACAACTTCTGTCACAACGCTTGCTCCCCTCCAGGCCCGGTCCATTGACCGGGCTTTGTTTTTTTATCTAGAGTGCCCAGCCTGACCGTTACAGAAGCCCGCATCATGCTGCCGCGCGCCGAACAGAAGCTACAGACCCGccc                                                  >  2:1016734/1‑149 (MQ=255)
                                                                                                gAAAAGAACAACTTCTGTCACAACGCTTGCTCCCCTCCAGGCCCGGTCCATTGACCGGGCTTTGTTTTTTTATCTAGAGTGCCCAGCCTGACCGTTACAGAAGCCCGCATCATGCTGCCGCGCGCCGAACAGAAGCTACAGACCCGCCAg                                                 >  1:1172049/1‑150 (MQ=255)
                                                                                                      aacaacTTCTGTCACAACGCTTGCTCCCCTCCAGGCCCGGTCCATTGACCGGGCTTTGTTTTTTTATCTAGAGTGCCCAGCCTGACCGTTa                                                                                                      <  1:185997/91‑1 (MQ=255)
                                                                                                      aacaacTTCTGTCACAACGCTTGCTCCCCTCCAGGCCCGGTCCATTGACCGGGCTTTGTTTTTTTATCTAGAGTGCCCAGCCTGACCGTTa                                                                                                      >  2:185997/1‑91 (MQ=255)
                                                                                                      aacaacTTCTGTCACAACGCTTGCTCCCCTCCAGGCCCGGTCCATTGACCGGGCTTTGTTTTTTTATCTAGAGTGCCCAGCCTGACCGTTACAGAAGCCCGCATCATGCTGCCGCGCGCCGAACAGAAGCTACAGACCCGCCAGGCCCTg                                           >  2:166543/1‑150 (MQ=255)
                                                                                                           cTTCTGTCACAACGCTTGCTCCCCTCCAGGCCCGGTCCATTGACCGGGCTTTGTTTTTTTATCTAGAGTGCCCAGCCTGACCGTTa                                                                                                      >  2:912500/1‑86 (MQ=255)
                                                                                                           cTTCTGTCACAACGCTTGCTCCCCTCCAGGCCCGGTCCATTGACCGGGCTTTGTTTTTTTATCTAGAGTGCCCAGCCTGACCGTTa                                                                                                      <  1:912500/86‑1 (MQ=255)
                                                                                                                   acaACGCTTGCTCCCCTCCAGGCCCGGTCCATTGACCGGGCTTTGTTTTTTTATCTAGAGTGCCCAGCCTGACCGTTACAGAAGccc                                                                                             <  2:122682/87‑1 (MQ=255)
                                                                                                                   acaACGCTTGCTCCCCTCCAGGCCCGGTCCATTGACCGGGCTTTGTTTTTTTATCTAGAGTGCCCAGCCTGACCGTTACAGAAGccc                                                                                             >  1:122682/1‑87 (MQ=255)
                                                                                                                     aaCGCTTGCTCCCCTCCAGGCCCGGTCCATTGACCGGGCTTTGTTTTTTTATCTAGAGTGCCCAGCCTGACCGTTACAGAAGCCCGCATCATGCTGCCGCGCGCCGAACAGAAGCTACAGACCCGCCAGGCCCTGCTCGATGCCgcctgc                            >  2:1183360/1‑150 (MQ=255)
                                                                                                                     aaCGCTTGCTCCCCTCCAGGCCCGGTCCATTGACCGGGCTTTGTTTTTTTATCTAGAGTGCCCAGCCTGACCGTTACAGAAGCCCGCATCATGCTGCCGCGCGCCGAACAGAAGCTACAGACCCGCCAGGCCCTGCTCGATGCCgcctgc                            <  2:1198086/150‑1 (MQ=255)
                                                                                                                              tCCCCTCCAGGCCCGGTCCATTGACCGGGCTTTGTTTTTTTATCTaga                                                                                                                         >  2:58899/1‑48 (MQ=255)
                                                                                                                              tCCCCTCCAGGCCCGGTCCATTGACCGGGCTTTGTTTTTTTATCTaga                                                                                                                         <  1:58899/48‑1 (MQ=255)
                                                                                                                                cccTCCAGGCCCGGTCCATTGACCGGGCTTTGTTTTTTTATCTAGAGTGCCCAGCCTGACCGTTACAGAAGCCCGCATCATGCTGCCGCGCGCCGAACAGa                                                                  >  2:465296/1‑101 (MQ=255)
                                                                                                                                cccTCCAGGCCCGGTCCATTGACCGGGCTTTGTTTTTTTATCTAGAGTGCCCAGCCTGACCGTTACAGAAGCCCGCATCATGCTGCCGCGCGCCGAACAGa                                                                  <  1:465296/101‑1 (MQ=255)
                                                                                                                                  cTCCAGGCCCGGTCCATTGACCGGGCTTTGTTTTTTTATCTAGAGTGCCCAGCCTGACCGTTACAGAAGCCCGCATCATGCTGCCGCGCGCCGAACAGAAGCTACAGACCCGCCAGGCCCTGCTCGATGCCGCCTGCCTGCTCATGgaga               <  2:673457/150‑1 (MQ=255)
                                                                                                                                   tCCAGGCCCGGTCCATTGACCGGGCTTTGTTTTTTTATCTAGAGTGCCCAGCCTGACCGTTACAGAAGCCCGCATCATGCTGCCGCGCGCCGAACAGAAGCTACAGACCCGCCAGGCCCTGCTCGATGCCGCCTGCCTGCTCATGga                 <  2:251752/147‑1 (MQ=255)
                                                                                                                                   tCCAGGCCCGGTCCATTGACCGGGCTTTGTTTTTTTATCTAGAGTGCCCAGCCTGACCGTTACAGAAGCCCGCATCATGCTGCCGCGCGCCGAACAGAAGCTACAGACCCGCCAGGCCCTGCTCGATGCCGCCTGCCTGCTCATGga                 >  1:251752/1‑147 (MQ=255)
                                                                                                                                     aaagCCCGGTCAATGGACCGGGCTTTGTTTTTTTATCTAGAGTGCCCAGCCTGACCGTTACAGAAGCCCGCATCATGCTGCCGCGCGCCGAACAGAAGCTACAGACCCGCCAGGCCCTGCTCGATGCCGCCTGCCTGCTCATGGAGAGTg            >  1:586324/4‑150 (MQ=255)
                                                                                                                                       agCCCGGTCAATGGACCGGGCTTTGTTTTTTTATCTAGAGTGCCCAGCCTGACCGTTACAGAAGCCCGCATCATGCTGCCGCGCGCCGAACAGAAGCTACAGACCCGCCAGGCCCTGCTCGATGCCGCCTGCCTGCTCATGGAGAGTGGc          >  1:131540/2‑150 (MQ=255)
                                                                                                                                           cGGTCCATTGACCGGGCTTTGTTTTTTTATCTAGAGTGCCCAGCCTGACCGTTACAGAAGCCCGCATCATGCTGCCGCGCGCCGAACAGAAGCTACAGACCCGCCAGGCCCTGCTCGATGCCGCCTGCCTGCTCATGGAGAGTGGCCGTg      <  2:1172049/150‑1 (MQ=255)
                                                                                                                                              tCCATTGACCGGGCTTTGTTTTTTTATCTAGAGTGCCCAGCCTGACCGTTACAGAAGCCCGCATCATGCTGCCGCGCGCCGAACAGAAGCTACAGACCCGCCAGGCCCTGCTCGATGCCGCCTGCCTGCTCATGGAGAGTGGCCGTGGtt   <  2:731958/150‑1 (MQ=255)
                                                                                                                                               ccATTGACCGGGCTTTGTTTTTTTATCTAGAGTGCCCAGCCTGACCGTTACAGAAGCCCGCATCATGCTGCCGCGCGCCGAACAGAAGCTACAGACCCGCCAGGCCCTGCTCGATGCCGCCTGCCTGCTCATGGAGAGTGGCCGTGGttt  <  1:166543/150‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                                    
CGGCTCCGATCGTCTGATCGAACAAGCTGGCCGTGTCGCTGAAGGTGGCTCGGATCGCCTGGTTGAACTCAGCCGTGTGAGCTGATCGCCATGGCCGAAAAGAACAACTTCTGTCACAACGCTTGCTCCCCTCCAGGCCCGGTCCATTGACCGGGCTTTGTTTTTTTATCTAGAGTGCCCAGCCTGACCGTTACAGAAGCCCGCATCATGCTGCCGCGCGCCGAACAGAAGCTACAGACCCGCCAGGCCCTGCTCGATGCCGCCTGCCTGCTCATGGAGAGTGGCCGTGGTTT  >  NC_002947/5525674‑5525966

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑