| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 5,532,085 | G→C | 9.6% | P79R (CCC→CGC) ‡ | braE ← | high‑affinity branched‑chain amino acid ABC transporter permease BraE |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 5,532,085 | 0 | G | C | 9.6% | 123.6 / 3.1 | 41 | P79R (CCC→CGC) ‡ | braE | high‑affinity branched‑chain amino acid ABC transporter permease BraE |
| Reads supporting (aligned to +/- strand): ref base G (21/16); new base C (2/2); total (23/18) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01 | |||||||||||
GCAGGTACTTGTTGGCGAAGATCGGGAAAACGATGGCAATCACGATCAGCGCGGGGATGATGTAACGCAGGCGCGACTTGTAGTCCGGTGCGGTCACATGCACGCCCGAGCCACCGCTGTCGAAGCCCAGGAGCATGCGCTGCCCGGGGGCAGTCTGCAGGAACAGGCTGAGCAGGAAGCGCCCCACCATCACACCGCCGACCAGCCAGGCCACGCGGCGCGGCTCGGCATTGAAGGTGTAGCCGTCGAGCACCACACCAACGACCGGACCAAACACGATAAGTGCCAGCAGG > NC_002947/5531939‑5532231 | gcagGTACTTGTTGGCGAAGATCGGGAAAACGATGGCAATCACGATCAGCGCGGGGATGATGTAACGCAGGCGCGACTTGTAGTCCGGTGCGGTCACATGCACGCCCGAGCCACCGCTGTCGAAGCCCAGGAGCATGCGCTGCCCggggg < 1:494528/150‑1 (MQ=255) gTACTTGTTGGCGAAGATCGGGAAAACGATGGCAATCACGATCAGCGCGGGGATGATGTAACGCAGGCGCGACTTGTAGTCCGGTGCGGTCACATGCACGCCCGAGCCACCGCTGTCGAAGCCCAGGAGCATGCGCTGCCCggggg < 1:777588/146‑1 (MQ=255) gTACTTGTTGGCGAAGATCGGGAAAACGATGGCAATCACGATCAGCGCGGGGATGATGTAACGCAGGCGCGACTTGTAGTCCGGTGCGGTCACATGCACGCCCGAGCCACCGCTGTCGAAGCCCAGGAGCATGCGCTGCCCggggg > 2:777588/1‑146 (MQ=255) gTACTTGTTGGCGAAGATCGGGAAAACGATGGCAATCACGATCAGCGCGGGGATGATGTAACGCAGGCGCGACTTGTAGTCCGGTGCGGTCACATGCACGCCCGAGCCACCGCTGTCGAAGCCCAGGAGCATGCGCTGCCCGGGGGCAGt > 2:1078615/1‑150 (MQ=255) tACTTGTTGGCGAAGATCGGGAAAACGATGGCAATCACGATCAGCGCGGGGATGATGTAACGCAGGCGCGACTTGTAGTCCGGTGCGGTCACATGCACGCCCGAGCCACCGCTGTCGAAGCCCAGGAGCATGCGCTGCCCGGGGGCAGTc > 2:124651/1‑150 (MQ=255) ttgttgGCGAAGATCGGGAAAACGATGGCAATCACGATCAGCGCGGGGATGATGTAACGCAGGCGCGACTTGTAGTCCGGTGCGGTCACATGCACGCCCGAGCCACCGCTGTCGAAGCCCAGGAGCATGCGCTGCCCggg > 2:311740/1‑140 (MQ=255) ttgttgGCGAAGATCGGGAAAACGATGGCAATCACGATCAGCGCGGGGATGATGTAACGCAGGCGCGACTTGTAGTCCGGTGCGGTCACATGCACGCCCGAGCCACCGCTGTCGAAGCCCAGGAGCATGCGCTGCCCggg < 1:311740/140‑1 (MQ=255) gCAATCACGATCAGCGCGGGGATGATGTAACGCAGGCGCGACTTGTAGTCCGGTGCGGTCACATGCACGCCCGAGCCACCGCTGTCGAAGCCCAGGAGCATGCGCTGCCCGGGGGCAGTCTGCAGGAACAGGCTGAGCa > 1:373482/1‑139 (MQ=255) gCAATCACGATCAGCGCGGGGATGATGTAACGCAGGCGCGACTTGTAGTCCGGTGCGGTCACATGCACGCCCGAGCCACCGCTGTCGAAGCCCAGGAGCATGCGCTGCCCGGGGGCAGTCTGCAGGAACAGGCTGAGCa < 2:373482/139‑1 (MQ=255) aaTCACGATCAGCGCGGGGATGATGTAACGCAGGCGCGACTTGTAGTCCGGTGCGGTCACATGCACGCCCGAGCCACCGCTGTCGAAGCCCAGGAGCATGCGCTGCCCCCGGGCAgcgca > 1:481821/1‑116 (MQ=255) aaTCACGATCAGCGCGGGGATGATGTAACGCAGGCGCGACTTGTAGTCCGGTGCGGTCACATGCACGCCCGAGCCACCGCTGTCGAAGCCCAGGAGCATGCGCTGCCCCCGGGCAgcgca < 2:481821/120‑5 (MQ=255) gATCAGCGCGGGGATGATGTAACGCAGGCGCGACTTGTAGTCCGGTGCGGTCACATGCACGCCCGAGCCACCGCTGTCGAAGCCCAGGAGCATGCGCTGCCCGGGGGCAGTCTGCa > 1:99685/1‑116 (MQ=255) gATCAGCGCGGGGATGATGTAACGCAGGCGCGACTTGTAGTCCGGTGCGGTCACATGCACGCCCGAGCCACCGCTGTCGAAGCCCAGGAGCATGCGCTGCCCGGGGGCAGTCTGCa < 2:99685/116‑1 (MQ=255) atgTAACGCAGGCGCGACTTGTAGTCCGGTGCGGTCACATGCACGCCCGAGCCACCGCTGTCGAAGCCCAGGAGCATGCGCTGCCCGGGGGCAGTCTGCAGGAACAgg > 2:214727/1‑108 (MQ=255) atgTAACGCAGGCGCGACTTGTAGTCCGGTGCGGTCACATGCACGCCCGAGCCACCGCTGTCGAAGCCCAGGAGCATGCGCTGCCCGGGGGCAGTCTGCAGGAACAgg < 1:214727/108‑1 (MQ=255) ggTAACGCAGGCGCGACTTGTAGTCCGGTGCGGCCACATGCACGCCCGAGCCACCGCTGTCGAAGCCCAGGAGCATGCGCTGCCCGGGGGCAGTCTGCAGGAACAGGCTGAGCAGGAAGCGCCCCACCATCACACCGCCGACCAGCCAgg < 1:112396/149‑1 (MQ=255) gcgcGACTTGTAGTCCGGTGCGGTCACATGCACGCCCGAGCCACCGCTGTCGAAGCCCAGGAGCATGCGCTGCCCCCGGGCAgcgca < 1:287445/87‑5 (MQ=255) gcgcGACTTGTAGTCCGGTGCGGTCACATGCACGCCCGAGCCACCGCTGTCGAAGCCCAGGAGCATGCGCTGCCCCCGGGCAgcgca > 2:287445/1‑83 (MQ=255) aCTTGTAGTCCGGTGCGGTCACATGCACGCCCGAGCCACCGCTGTCGAAGCCCAGGAGCATGCGCTGCCCGGGGGCAGt < 2:1057628/79‑1 (MQ=255) aCTTGTAGTCCGGTGCGGTCACATGCACGCCCGAGCCACCGCTGTCGAAGCCCAGGAGCATGCGCTGCCCGGGGGCAGt > 1:1057628/1‑79 (MQ=255) cTTGTAGTCCGGTGCGGTCACATGCACGCCCGATCCACCGCTGTCGAAGCCCAGGAGCATGCGCTGCCCGGGGGCAGTCTGCAGGAACAGGCTGAGCAGGAAGCGCCCCACCATCACACCGCCGACCAGCCAGGCCACGCGGCGCGGCTc > 2:531214/1‑150 (MQ=255) cTTGTAGTCCGGTGCGGTCACATGCACGCCCGAGCCACCGCTGTCGAAGCCCAGGAGCATGCGCTGCCCGGGGGCAGTCTGCAGGAACAGGCTGAGCAGGAAGCGCCCCACCATCACACCGCCTACCCGCCAGGCCACGCGGCGCGGCTc < 2:138901/150‑1 (MQ=255) ttGTAGTCCGGTGCGGTCACATGCACGCCCGAGCCACCGCTGTCGAAGCCCAGGAGCATGCGCTGCCCGGGGGCAGTCTGCAGGAACAGGCTGAGCAGGAAGCGCCCCACCATCACACCGCCGACCAGCCAGGCCACGCGGCGCGGCTCg > 1:586773/1‑150 (MQ=255) gTAGTCCGGTGCGGTCACATGCACGCCCGAGCCACCGCTGTCGAAGCCCAGGAGCATGCGCTGCCCGGGGGCAGTCTg < 2:485121/78‑1 (MQ=255) gTAGTCCGGTGCGGTCACATGCACGCCCGAGCCACCGCTGTCGAAGCCCAGGAGCATGCGCTGCCCGGGGGCAGTCTg > 1:485121/1‑78 (MQ=255) gTGCGGTCACATGCACGCCCGAGCCACCGCTGTCGAAGCCCAGGAGCATGCGCTGCCCGGGGGCAGTCTGCAGGAACAGGCTGAGCAGGaa < 1:276629/91‑1 (MQ=255) gTGCGGTCACATGCACGCCCGAGCCACCGCTGTCGAAGCCCAGGAGCATGCGCTGCCCGGGGGCAGTCTGCAGGAACAGGCTGAGCAGGaa > 2:276629/1‑91 (MQ=255) ggTCACATGCACGCCCGAGCCACCGCTGTCGAAGCCCAGGAGCATGCGCTGCCCGGGGGCAGTCTGCAGGAACAGGCTGa > 2:844836/1‑80 (MQ=255) ggTCACATGCACGCCCGAGCCACCGCTGTCGAAGCCCAGGAGCATGCGCTGCCCGGGGGCAGTCTGCAGGAACAGGCTGa < 1:844836/80‑1 (MQ=255) aTGCACGCCCGAGCCACCGCTGTCGAAGCCCAGGAGCATGCGCTGCCCGGGGGCAGTCTGCAGGAACAGGCTGAGCAGGAAGCGCCCCACCATCACACCGCCGACCAGCCAGGCCACGCGGCGCGGCTCGGCATTGAAGGTGTAGCCGTc > 1:1029546/1‑150 (MQ=255) ccGAGCCACCGCTGTCGAAGCCCAGGAGCATGCGCTGCCCGGGGGCAGTCTGCCGGCACATGCTGAGCAGGAGACGCCCCACCATCACACCGCCGACCAGCCAGGCCACGCGGCGCGGCTCGGCATTGAAGGTGTAGCCGTCGAGcacca > 1:451113/1‑150 (MQ=255) cACCGCTGTCGAAGCCCAGGAGCATGCGCTGCCCGGGGGCAGTCTGCAGGAACAGGCTGAGCAGGAAGCGCCCCACCATCACACCGCCGACCAGCCAGGCCACGCGGCGCGGCTCGGCATTGAAGGTGTAGCCGTCGAGCACCACACCaa > 2:518538/1‑150 (MQ=255) aCCGCTGTCGAAGCCCAGGAGCATGCGCTGCCCGGGGGCAGTCTGCAGGAACAGGCTGAGCAGGAAGCGCCCCACCATCACACCGCCGACCAGCCAGGCCACGCGGCGCGGCTc > 2:309087/1‑114 (MQ=255) aCCGCTGTCGAAGCCCAGGAGCATGCGCTGCCCGGGGGCAGTCTGCAGGAACAGGCTGAGCAGGAAGCGCCCCACCATCACACCGCCGACCAGCCAGGCCACGCGGCGCGGCTc < 1:309087/114‑1 (MQ=255) aCCGCTGTCGAAGCCCAGGAGCATGCGCTGCCCGGGGGCAGTCTGCAGGAACAGGCTGAGCAGGAAGCGCCCCACCATCACACCGCCGACCAGCCAGGCCACGCGGCGCGGCTCGGCATTGAAGGTGTAGCCGTCGAGCACCACACCAAc > 2:523932/1‑150 (MQ=255) cTGTCGAAGCCCAGGAGCATGCGCTGCCCGGGGGCAGTCTGCAGGAACAGGCTGAGCAGGAAGCGCCCCACCATCACACCGCCGACCAGCCAGGCCACGCGGCGCGGCTCGGCATTGAAGGTGTAGCCGTCGAGCACCACACCAACGAcc < 2:1029546/150‑1 (MQ=255) cTGTCGAAGCCCAGGAGCATGCGCTGCCCGGGGGCAGTCTGCAGGAACAGGCTGAGCAGGAAGCGCCCCACCATCACACCGCCGACCAGCCAGGCCACGCGGCGCGGCTCGGCATTGAAGGTGTAGCCGTCGAGCACCACACCAACGAcc < 2:586773/150‑1 (MQ=255) cGAAGCCCAGGAGCATGCGCTGCCCGGGGGCAGTCTGCAGGAACAGGCTGAGCAGGAAGCGCCCCACCATCACACCGCCGACCAGCCAGGCCACGCGGCGCGGCTCGGCATTGAAGGTGTAGCCGTCGAGCACCACACCAACGACCGGAc < 1:725484/150‑1 (MQ=255) gAAGCCCAGGAGCATGCGCTGCCCGGGGGCAGTCTGCAGGAACAGGCTGAGCAGGAAGCGCCCCACCATCACACCGCCGACCAGCCAGGCCACGCGGCGCGGCTCGGCATTGAAGGTGTAGCCGTCGAGCACCACACCAACGACCGGAcc > 2:261477/1‑150 (MQ=255) gCCCAGGAGCATGCGCTGCCCGGGGGCAGTCTGCAGGAACAGGCTGAGCAGGAAGCGCCCCACCATCACACCGCCGACCAGCCAGGCCACGCGGCGCGGCTCGGCATTGAAGGTGTAGCCGTCGAGCACCACACCAACGACCGGACCaaa > 2:706233/1‑150 (MQ=255) ccAGGAGCATGCGCTGCCCGGGGGCAGTCTGCAGGAACAGGCTGAGCAGGAAGCGCCCCACCATCACACCGCCGACCAGCCAGGCCACGCGGCGCGGCTCGGCATTGAAGGTGTAGCCGTCGAGcaccacacc > 2:879428/1‑133 (MQ=255) ccAGGAGCATGCGCTGCCCGGGGGCAGTCTGCAGGAACAGGCTGAGCAGGAAGCGCCCCACCATCACACCGCCGACCAGCCAGGCCACGCGGCGCGGCTCGGCATTGAAGGTGTAGCCGTCGAGcaccacacc < 1:879428/133‑1 (MQ=255) ccAGGAGCATGCGCTGCCCGGGGGCAGTCTGCAGGAACAGGCTGAGCAGGAAGCGCCCCACCATCACACCGCCGACCAGCCAGGCCACGCGGCGCGGCTCGGCATTGAAGGTGTAGCCGTCGAGCACCACACCAACGACCGGACCAAaca < 1:523932/150‑1 (MQ=255) gcgcTGCCCGGGGGCAGTCTGCAGGAACAGGCTGAGCAGGAAGCGCCCCACCATCACACCGCCGACCAGCCAGGCCACGCGGCGCGGCTCGGCATTGAAGGTGTAGCCGTCGAGCACCACACCAACGACCGGACCAAACACGATAAGTGc < 1:518538/150‑1 (MQ=255) cccGGGGGCAGTCTGCAGGAACAGGCTGAGCAGGAAGCGCCCCACCATCACACCGCCGACCAGCCAGGCCACGCGGCGCGGCTCGGCATTGAAGGTGTAGCCGTCGAGCACCACACCAACGACCGGACCAAACACGATAAGTGCcagcag > 2:232027/1‑150 (MQ=255) ccGGGGGCAGTCTGCAGGAACAGGCTGAGCAGGAAGCGCCCCACCATCACACCGCCGACCAGCCAGGCCACGCGGCGCGGCTCGGCATTGAAGGTGTAGCCGTCGAGCACCACACCAACGACCGGACCAAACACGATAAGTGCCAGCAgg > 1:257019/1‑150 (MQ=255) | GCAGGTACTTGTTGGCGAAGATCGGGAAAACGATGGCAATCACGATCAGCGCGGGGATGATGTAACGCAGGCGCGACTTGTAGTCCGGTGCGGTCACATGCACGCCCGAGCCACCGCTGTCGAAGCCCAGGAGCATGCGCTGCCCGGGGGCAGTCTGCAGGAACAGGCTGAGCAGGAAGCGCCCCACCATCACACCGCCGACCAGCCAGGCCACGCGGCGCGGCTCGGCATTGAAGGTGTAGCCGTCGAGCACCACACCAACGACCGGACCAAACACGATAAGTGCCAGCAGG > NC_002947/5531939‑5532231 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |