| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 5,545,683 | T→C | 20.5% | intergenic (‑775/+102) | rpsF ← / ← rlmB | 30S ribosomal protein S6/23S rRNA (guanosine(2251)‑2'‑O)‑methyltransferase RlmB |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 5,545,683 | 0 | T | C | 20.5% | 72.5 / 14.3 | 39 | intergenic (‑775/+102) | rpsF/rlmB | 30S ribosomal protein S6/23S rRNA (guanosine(2251)‑2'‑O)‑methyltransferase RlmB |
| Reads supporting (aligned to +/- strand): ref base T (17/14); new base C (4/4); total (21/18) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.98e-01 | |||||||||||
ATAGCAAAGTATTCGGCGGCTGACCTACCCGGATCAGTCGGCGAATGCTTTTCTAGATCTCGATGTCAGTGCAGATCTGACGTCATGCGCATCCTGTTTGCTGTGCGACAGCGCAGCCCGGAAGGGCTGGGTGATCTCCTACAGGGGAACGCAATCACCCTGTAGGAGCGGGTTCACCCGCGAAGAGGCCGGAACAGGCTGAGAGTCAGCGCTTGACCTGCCGCTGACGCACGGCTTCGAACAGACAAACC > NC_002947/5545580‑5545830 | aTAGCAAAGTATTCGGCGGCTGACCTACCCGGATCAGTCGGCGAATGCTTTTCTAGATCTCGATGTCAGTGCAGATCTGACGTCATGCGCATCCTGTTTGCTGTGCGACAGCGCAGCCCGGAAGGGCTGGGTGATCTCCTACAGGGGAAc > 2:38564/1‑150 (MQ=38) tATTCGGCGGCTGACCTACCCGGATCAGTCGGCGAATGCTTTTCTAGATCTCGATGTCAGTGCAGATCTGACGTCATGCGCATCCTGTTTGCTGTGCGACAGCGCAGCCCGGAAGGGCTGGGTGATCTCCTACAGGGGAACGCAATCAcc > 1:971829/1‑150 (MQ=255) ttCGGCGGCTGACCTACCCGGATCAGTCGGCGAATGCTTTTCTAGATCTCGATGTCAGTGCAGATCTGACGTCATGCGCATCCTGTTTGCTGTGCGACAGCGCAGCCCGGAAGGGCTGGGTGATCTCCTACAGGGGAACGCAATCACCCt > 2:978228/1‑150 (MQ=255) ggcggttggcccggtagggccagccgccttttctttttcaaaatgtcagcgtcgctgcggaactgaagccgtacacctcaTGTTTGCTGCGCGACAGCGCAGCCCGGAAggg > 1:137847‑M2/81‑112 (MQ=255) ggcggttggcccggtagggccagccgccttttctttttcaaaatgtcagcgtcgctgcggaactgaagccgtacacctcaTGTTTGCTGCGCGACAGCGCAGCCCGGAAggg < 2:137847‑M2/32‑1 (MQ=255) gcggttggcccggtagggccagccgccttttctttttcaaaatgtcagcgtcgctgcggaactgaagccgtacacctcaTGTTTGCTGCGCGACAGCGCAGCCCGGaa > 2:532413‑M2/80‑108 (MQ=255) gcggttggcccggtagggccagccgccttttctttttcaaaatgtcagcgtcgctgcggaactgaagccgtacacctcaTGTTTGCTGCGCGACAGCGCAGCCCGGaa < 1:532413‑M2/29‑1 (MQ=255) gcggcTGACCTACCCGGATCAGTCGGCGAATGCTTTTCTAGATCTCGATGTCAGTGCAGATCTGACGTCATGCGCATCCTGTTTGCTGTGCGACAGCGCAGCCCGGAAGGGCTGGGTGATCTCCTACAGGGGAACGCAATCACCCTGTAg < 2:1065330/150‑1 (MQ=255) ggttggcccggtagggccagccgccttttctttttcaaaatgtcagcgtcgctgcggaactgaagccgtacacctcaTGTTTGCTGCGCGACAGCGCAGCCCGGAAGGGCTGGGTGAt < 2:789652‑M2/41‑1 (MQ=255) ggttggcccggtagggccagccgccttttctttttcaaaatgtcagcgtcgctgcggaactgaagccgtacacctcaTGTTTGCTGCGCGACAGCGCAGCCCGGAAGGGCTGGGTGAt > 1:789652‑M2/78‑118 (MQ=255) ggttggcccggtagggccaaccgccttttctttttcaaaatgtcagcgtcgctgcggaactgaagccgtacacctcaTGTTTGCTGCGCGACAGCGCAGCCCGGAAGGGCTGGGTg > 2:884982‑M2/78‑116 (MQ=255) ggttggcccggtagggccaaccgccttttctttttcaaaatgtcagcgtcgctgcggaactgaagccgtacacctcaTGTTTGCTGCGCGACAGCGCAGCCCGGAAGGGCTGGGTg < 1:884982‑M2/39‑1 (MQ=255) tGACCTACCCGGATCAGTCGGCGAATGCTTTTCTAGATCTCGATGTCAGTGCAGATCTGACGTCATGCGCATCCTGTTTGCTGTGAGACAGCGCAGCCCGGAAGGGCTGGGTGATCTCCTACAGGGGAACGCAATCACCCTGTAGGAGCg > 1:727595/1‑150 (MQ=255) cTACCCGGATCAGTCGGCGAATGCTTTTCTAGATCTCGATGTCAGTGCAGATCTGACGTCATGCGCATCCTGTTTGCTGTGCGACAGCGCAGCCCGGAAGGGCTGGGTGATCTCCTACAGGGGAACGCAATCACCCTGTAGGAGCGGGtt > 2:433494/1‑150 (MQ=255) cAGTCGGCGAATGCTTTTCTAGATCTCGATGTCAGTGCAGATCTGACGTCATGCGCATCCTGTTTGCTGTGCGACAGCGCAGCCCGGAAGGGCTGGGTGATCTCCTACAGGGGAACGCAATCACCCTGTAGGAGCGGGTTCACCCGCGaa > 2:419507/1‑150 (MQ=255) gTCGGCGAATGCTTTTCTAGATCTCGATGTCAGTGCAGATCTGACGTCATGCGCATCCTGTTTGCTGTGCGACAGCGCAGCCCGGAAGGGCTGGGTGATCTCCTACAGGGGAACGCAATCACCCTGTAGGAGCGGGTTCACCCGCGAaga < 2:971829/150‑1 (MQ=255) aTGCTTTTCTAGATCTCGATGTCAGTGCAGATCTGACGTCATGCGCATCATGTTTGCTGTGCGACAGCGCAGCCCGGAAGGGCTGGGTGATCTCCTACAGGGGAACGCAATCACCCTGTAGGAGCGGGTTCACCCGCGAAGAGGCCGGaa < 2:61494/150‑1 (MQ=255) cTTTTCTAGATCTCGATGTCAGTGCAGATCTGACGTCATGCGCATCCTGTTTGCTGTGCGACAGCGCAGCCCGGAAGGGCTGGGTGATCTCCTACAGGGGAACGCAATCa < 1:856578/110‑1 (MQ=255) cTTTTCTAGATCTCGATGTCAGTGCAGATCTGACGTCATGCGCATCCTGTTTGCTGTGCGACAGCGCAGCCCGGAAGGGCTGGGTGATCTCCTACAGGGGAACGCAATCa > 2:856578/1‑110 (MQ=255) tttCTAGATCTCGATGTCAGTGCAGATCTGACGTCATGCGCATCCTGTTTGCTGTGCGACAGCGCAGCCCGGAAGGGCTGGGTGATCTCCTACAGGGGAACGCAATCACCCTGTAGGAGCGGGTTCACCCGCGAAGAGGCCGGAACAGGc < 1:419507/150‑1 (MQ=255) aGATCTCGATGTCAGTGCAGATCTGACGTCATGCGCATCCTGTTTGCTGTGCGACAGCGCAGCCCGGAAGGGCTGGGTGATCTCCTACAGGGGAACGCAATCACCCTGTAGGAGCGGGTTCACCCGCGAAGAGGCCGGAACAgg < 1:34754/144‑1 (MQ=255) aGATCTCGATGTCAGTGCAGATCTGACGTCATGCGCATCCTGTTTGCTGTGCGACAGCGCAGCCCGGAAGGGCTGGGTGATCTCCTACAGGGGAACGCAATCACCCTGTAGGAGCGGGTTCACCCGCGAAGAGGCCGGAACAgg > 2:34754/1‑144 (MQ=255) ctcGATGTCAGTGCAGATCTGACGTCATGCGCATCCTGTTTGCTGTGCGACAGCGCAGCCCGGAAGGGCTGGGTGATCTCCTACAGGGGAACGCAATCACCCTGTAGGAGCGGGTTCACCAGCGCTGAGGCCGGAACAGGCTGAGAGTCa < 1:163842/150‑1 (MQ=255) aTCTGACGTCATGCGCATCCTGTTTGCTGTGCGACAGCGCAGCCCGGAAGGGCTGGGTGATCTCCTACAGGGGAACGCCGGTTGCCTGTAGGAGCGGGTTCACCCGCGAAGAGGCCGGAACAGGCTGAGAGTCAGCGCTCGACCTGCCGc > 1:979807/1‑150 (MQ=25) tCTGACGTCATGCGCATCCTGTTTGCTGTGCGACAGCGCAGCCCGGAAGGGCTGGGTGATCTCCTACAGGGGAACGCAATCACCCTGTAGGAGCGGGTTCACCCGCGAAGAGGCCGGAACAGGCTGAGAGTCAGCGCTTGACCTGCCGCt > 2:291939/1‑150 (MQ=255) cTGACGTCATGCGCATCCTGTTTGCTGTGCGACAGCGCAGCCCGGAAGGGCTGGGTGATCTCCTACAGGGGAACGCAATCACCCTGTAGGAGCGGGTTCACCCGCGAAGAGGCCGGAACAGGCTGAGAGTCAGCGCTTGACCTGCCGCTg > 2:438617/1‑150 (MQ=255) gACGTCATGCGCATCCTGTTTGCTGTGCGACAGCGCAGCCCGGAAGGGCTGGGTGATCTCCTACAGGGGAACGCAATCACCCTGTAGGAGCGGGTTCACCCGCGAAGAGGCCGGAACAGGCTGAGAGTCAGCGCTTGACCTGCCGCTGAc > 1:920904/1‑150 (MQ=255) tCATGCGCATCCTGTTTGCTGTGCGACAGCGCAGCCCGGAAGGGCTGGGTTATCTCCTACAGGGGAACGCAATCACCCTGTAGGAGCGGGTTCACCCGCGAAGAGGCCGGAACAGGCTGAGAGTCAGCGCTTGACCTGCCGCTGACGCAc > 1:662910/1‑150 (MQ=255) aTGCTCATCATGTTTGTTTTGCGACAGCGCAGCCGGGAAGGGCTGGGTGATCTGTTACAGGGGAACGCAATCACGCTGTAGGAGCGGGTTCACCGGCGAAGAGTCCGGAACAGGCTGAGAGTCTGCGCTTGACCTGCCGCTGACggtcgg < 2:283637/150‑6 (MQ=255) gcgcATCCTGTTTGCTGTGCGACAGCGCAGCCCGGAAGGGCTGGGTGATCTCCTACAGGGGAACGCAATCACCCTGTAGGAGCGGGTTCACCCGCGAAGAGGCCGGAACAGGCTGAGAGTCAGCGCTTGACCTGCCGCTGACGCACGGCt > 1:58048/1‑150 (MQ=255) cgcATCCTGTTTGCTGTGCGACAGCGCAGCCCGGAAGGGCTGGGTGATCTCCTACAGGGGAACGCAATCACCCTGTAGGAGCGGGTTCACCCGCGAAGAGGCCGGAACAGGCTGAGAGTCAGCGCTTGACCTGCCGCTGACGCACGGCtt < 1:438617/150‑1 (MQ=255) gcATCCTGTTTGCTGTGAGACAGCGCAGCCCGGAAGGGCTGGGTGATCTCCTACAGGGGAACGCAATCACCCTGTAGGAGCGGGTTCACCCGCGAAGAGGCCGGAACAGGCTGAGAGTCAGCGCTTGACCTGCCGCTGACGCACGGCTTc < 2:727595/150‑1 (MQ=255) aTCCTGTTTGCTGTGCGACAGCGCAGCCCGGAAGGGCTGGGTGATCTCCTACAGGGGAACGCAATCACCCGTTAGGAGCGGGTTCACCCGCGAAGAGGCCGGAACAGGCTGAGAGTCAGCGCTTGACCTGCCGCTGACGCACGGCTTCGa > 1:84426/1‑150 (MQ=255) tCCTGTTTGCTGTGCGACAGCGCAGCCCGGAAGGGCTGGGTGATCTCCTACAGGGGAACGCAATCACCCTGTAGGAGCGGGTTCACCCGCGAAGAGGCCGGAACAGGCTGAGAGTCAGCGCTTGACCTGCCGCTGACGCACGGCTTCGaa < 1:1120571/150‑1 (MQ=255) tCCTGTTTGCTGTGCGACAGCGCAGCCCGGAAGGGCTGGGTGATCTCCTACAGGGGAACGCAATCACCCTGTAGGAGCGGGTTCACCCGCGAAGAGGCCGGAACAGGCTGAGAGTCAGCGCTTGACCTGCCGCTGACGCACGGCTTCGaa > 2:398677/1‑150 (MQ=255) cTGTTTGCTGTGCGACAGCGCAGCCCGGAAGGGCTGGGTGATCTCCTACAGGGGAACGCAATCACCCTGTAGGAGCCGGTTCACCCGCGAAGAGGCCGGAACAGGCTGAGAGTCAGCGCTTGACCTGCCGCTGACGCACGGCTTCGAACa < 1:978228/150‑1 (MQ=255) cTGTTTGCTGTGAGACAGCGCAGCCCGGAAGGGCTGGGTGATCTCCTACAGGGGAACGCAATCACCCTGTAGGAGCGGGTTCACCCGCGAAGAGGCCGGAACAGGCTGAGAGTCAGCGCTTGACCTGCCGCTGACGCACGGCTTCGAACa > 2:1013571/1‑150 (MQ=255) tGTTTGCTGTGCGACAGCGCAGCCCGGAAGGGCTGGGTGATCTCCTACAGGGGAACGCAATCACCCTGTAGGAGCGGGTTCACCCGCGAAGAGGCCGGAACAGGCTGAGAGTCAGCGCTTGACCTGCCGCTGACGCACGGCTTCGAACAg < 1:1133110/150‑1 (MQ=255) gTTTGCTGTGCGACAGCGCAGCCCGGAAGGGCTGGGTGATCTCCTACAGGGGAACACAATCACCCTGTAGGAGCGGGTTCACCCGCGAAGAGGCCGGAACAGGCTGAGAGTCAGCGCTTGACCTGCCGCTGACGCACGGCTTCGAACAGa < 2:526501/150‑1 (MQ=255) tgtgCGACAGCGCAGCCCGGAAGGGCTGGGTGATCTCCTACAGGGGAACGCAATCACCCTGTAGGAGCGGGTTCACCCGCGAAGAGGCCGGAACAGGCTGAGAGTCAGCGCTTGACCTGCCg < 2:38241/122‑1 (MQ=255) tgtgCGACAGCGCAGCCCGGAAGGGCTGGGTGATCTCCTACAGGGGAACGCAATCACCCTGTAGGAGCGGGTTCACCCGCGAAGAGGCCGGAACAGGCTGAGAGTCAGCGCTTGACCTGCCg > 1:38241/1‑122 (MQ=255) tgtgCGACAGCGCAGCCCGGAAGGGCTGAGTGATCTCCTACAGGGGAACGCAATCACCCTGTAGGAGCGGGTTCACCCGCGAAGAGGCCGGAACAGGCTGAGAGTCAGCGCTTGACCTGCCGCTGACGCACGGCTTCGAACAGACAAAcc > 2:261173/1‑150 (MQ=255) gtgCGACAGCGCAGCCCGGAAGGGCTGGGTGATCTCCTACAGGGGAACGCAATCACCCTGTAGGAGCGGGTTCACCCGCGAAGAGGCCGGAACAgg < 2:1032517/96‑1 (MQ=255) gtgCGACAGCGCAGCCCGGAAGGGCTGGGTGATCTCCTACAGGGGAACGCAATCACCCTGTAGGAGCGGGTTCACCCGCGAAGAGGCCGGAACAgg > 1:1032517/1‑96 (MQ=255) | ATAGCAAAGTATTCGGCGGCTGACCTACCCGGATCAGTCGGCGAATGCTTTTCTAGATCTCGATGTCAGTGCAGATCTGACGTCATGCGCATCCTGTTTGCTGTGCGACAGCGCAGCCCGGAAGGGCTGGGTGATCTCCTACAGGGGAACGCAATCACCCTGTAGGAGCGGGTTCACCCGCGAAGAGGCCGGAACAGGCTGAGAGTCAGCGCTTGACCTGCCGCTGACGCACGGCTTCGAACAGACAAACC > NC_002947/5545580‑5545830 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |