Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,602,473 G→T 9.3% L354M (CTG→ATG)  PP_4924 ← subtilase family serine protease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,602,4730GT 9.3% 131.4 / 2.4 43L354M (CTG→ATG) PP_4924subtilase family serine protease
Reads supporting (aligned to +/- strand):  ref base G (20/19);  new base T (2/2);  total (22/21)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.58e-01

AGCTGGCTGACGTTGCTCACCTGGCCGGTACCGTTGTCATGCACCAGCATGCCGGTCAGCCGGTCTGCCCAGCCGGTGCGGCGCAGTGAACCACTGTTATCGATATCGAACAGTACGCCTTCCTCGATACCCGTCAGACCAACGCCCTTGCCGCTGAGGTCGAGCAAGAGCGGGTCGACAAACGTGTTCACCGTGGTCATTGCTGCCAACCCGTTGAGTACCACGGCATTGCGCAGGCTGAAATCCGGTTGTTCATTGCCATCACGATAGAACTGAGCCAGATGC  >  NC_002947/5602337‑5602621
                                                                                                                                        |                                                                                                                                                    
aGCTGGCTGACGTTGCTCACCTGGCCGGTACCGTTGTCATGCACCAGCATGCCGGTCAGCCGGTCTGCCCAGCCGGTGCGGCGCAGTGAACCACTGTTATCGATATCGAACAGTACGCCTTCCTCGATACCCGTCAGACCAACGCCCTTg                                                                                                                                         <  1:154322/150‑1 (MQ=255)
  ctggctgACGTTGCTCACCTGGCCGGTACCGTTGTCATGCACCAGCATGCCGGTCAGCCGGTCTGCCCAGCCGGTGCGGCGCAGTGAACCACTGTTATCGATATCGAACAGTACGCCTTCCTCGATACCCGTCAGACCAACGCCCTTGcc                                                                                                                                       >  2:770359/1‑150 (MQ=255)
       tgACGTTGCTCACCTGGCCGGTACCGTTGTCATGCACCAGCATGCCGGTCAGCCGGTCTGCCCAGCCGGTGCGGCGCAGTGAACCACTGTTATCGATATCGAACAGTACGCCTTCCTCGATACCCGTCAGACCAACGCCCTTGCCGCTGa                                                                                                                                  <  1:1015431/150‑1 (MQ=255)
       tgACGTTGCTCACCTGGCCGGTACCGTTGTCATGCACCAGCATGCCGGTCAGCCGGTCTGCCCAGCCGGTGCGGCGCAGTGAACCACTGTTATCGATATCGAACAGTACGCCTTCCTCGATACCCGTCAGACCAACGCCCTTGCCGCTGa                                                                                                                                  <  1:987962/150‑1 (MQ=255)
           gTTGCTCACCTGGCCGGTACCGTTGTCATGCACCAGCATGCCGGTCAGCCGGTCTGCCCAGCCGGTGCGGCGCAGTGAACCACTGTTATCGATATCGAACAGTACGCCTTCCTCGATACCCGTCAGACCAACGCCCTTGCCGCTGAGGTc                                                                                                                              <  1:770359/150‑1 (MQ=255)
                  aCCTGGCCGGTACCGTTGTCATGCACCAGCATGCCGGTCAGCCGGTCTGCCCAGCCGGTGCGGCGCAGTGAACCACTGTTATCGATATCGAACAGTACGCCTTCCTCGATACCCGTCAGACCAACGCCCTTGCCGCTGAGGTCGAGCAag                                                                                                                       >  2:520422/1‑150 (MQ=255)
                  aCCTGGCCGGTACCGTTGTCATGCACCAGCATGCCGGTCAGCCGGTCTGCCCAGCCGGTGCGGCGCAGTGAACCACTGTTATCGATATCGAACAGTACGCCTTCCTCCAGTGCTGTCAGACCAACGCCCTTGCCGCTGAGGTCGAGCAag                                                                                                                       >  1:615962/1‑150 (MQ=255)
                  aCCTGGCCGGTACCGTTGTCATGCACCAGCATGCCGGCAAGCCGGTATGCCCAACCGCTGCGGCGCAGTGAACCACTGTTATCGATATCGAACAGTACGCCTTGCTCGATAACCGTCCGACCAACGCCCTTGCCGCTAAGGTCGAGCAag                                                                                                                       >  2:348551/1‑150 (MQ=255)
                         cGGTACCGTTGTCATGCACCAGCATGCCGGTCAGCCGGTCTGCCCAGCCGGTGCGGCGCAGTGAACCACTGTTATCGATATCGAACAGTACGCCTTCCTCGATACCCGTCAGACCAACGCCCTTGCCGCTGAGGTCGAGCAAGAGCGGGt                                                                                                                <  1:520422/150‑1 (MQ=255)
                             aCCGTTGTCATGCACCAGCATGCCGGTCAGCCGGTCTGCCCAGCCGGTGCGGCGCAGTGAACCACTGTTATCGATATCGAACAGTACGCCTTCCTCGATACCCGTCAGAc                                                                                                                                                    <  2:725765/110‑1 (MQ=255)
                             aCCGTTGTCATGCACCAGCATGCCGGTCAGCCGGTCTGCCCAGCCGGTGCGGCGCAGTGAACCACTGTTATCGATATCGAACAGTACGCCTTCCTCGATACCCGTCAGAc                                                                                                                                                    >  1:725765/1‑110 (MQ=255)
                              ccGTTGTCATGCGCCAGCATGCCGGTCAGCCGGTCTGCCCAGCCGGTGCGGCGCAGTGAACCACTGTTATCGATATCGAACAGTACGCCTTCCTCGATACCCGTCAGACCAACGCCCTTGCCGCTGAGGTCGAGCAAGAGCGGGTCGACa                                                                                                           >  2:1018517/1‑150 (MQ=255)
                                 ttGTCATGCACCAGCATGCCGGTCAGCCGGTCTGCCCAGCCGGTGCGGCGCAGTGAACCACTGTTATCGATATCGAACAGTACGCCTTCCTCGATACCCGTCAGACCAACGCCCTTGCCGCTGAGGTCGAGCAAGAGCGGGTCGACAAAc                                                                                                        >  1:692741/1‑150 (MQ=255)
                                    tCATGCACCAGCATGCCGGTCAGCCGGTCTGCCCAGCCGGTGCGGCGCAGTGAACCACTGTTATCGATATCGAACAGTACGCCTTCCTCGATACCCGTCAGACCAACGCCCTTGCCGCTGAGGTCGAGCAAGAGCGGGTCGACAAACgtg                                                                                                     <  2:227135/150‑1 (MQ=255)
                                       tGCACCAGCATGCCGGTCAGCCGGTCTGCCCAGCCGGTGCGGCGCAGTGAACCACTGTTATCGATATCGAACAGTACGCCTTCCTCGATACCCGTCAGACCAACGCCCTTGCCGCTGAGGTCGAg                                                                                                                           >  2:710139/1‑125 (MQ=255)
                                       tGCACCAGCATGCCGGTCAGCCGGTCTGCCCAGCCGGTGCGGCGCAGTGAACCACTGTTATCGATATCGAACAGTACGCCTTCCTCGATACCCGTCAGACCAACGCCCTTGCCGCTGAGGTCGAg                                                                                                                           <  1:710139/125‑1 (MQ=255)
                                         cACCAGCATGCCGGTCAGCCGGTCTGCCCAGCCGGTGCGGCGCAGTGAACCACTGTTATCGATATCGAACAGTACGCCTTCCTCGATACCCGTCAGACCAACGCCCTTGCCGCTGAGGTCGAg                                                                                                                           >  1:763821/1‑123 (MQ=255)
                                         cACCAGCATGCCGGTCAGCCGGTCTGCCCAGCCGGTGCGGCGCAGTGAACCACTGTTATCGATATCGAACAGTACGCCTTCCTCGATACCCGTCAGACCAACGCCCTTGCCGCTGAGGTCGAg                                                                                                                           <  2:763821/123‑1 (MQ=255)
                                           ccAGCATGCCGGTCAGCCGGTCTGCCCAGCCGGTGCGGCGCAGTGAACCACTGTTATCGATATCGAACAGTACGCCTTCCTCGATACCCGTCAGACCAACGCCCTTGCCGCTGAGGTCGAGCAAGAGCGGGTCGACAAAc                                                                                                        <  1:770423/140‑1 (MQ=255)
                                           ccAGCATGCCGGTCAGCCGGTCTGCCCAGCCGGTGCGGCGCAGTGAACCACTGCTATCGATATCGAACAGTACGCCTTCCTCGATACCCGTCAGACCAACGCCCTTGCCGCTGAGGTCGAGCAAGAGCGGGTCGACAAAc                                                                                                        >  2:770423/1‑140 (MQ=255)
                                                    cGGTCAGCCGGTCTGCCCAGCCGGTGCGGCGCAGTGAACCACTGTTATCGATATCGAACAGTACGCCTTCCTCGATACCCGTCAGACCAACGccc                                                                                                                                            <  2:71641/95‑1 (MQ=255)
                                                    cGGTCAGCCGGTCTGCCCAGCCGGTGCGGCGCAGTGAACCACTGTTATCGATATCGAACAGTACGCCTTCCTCGATACCCGTCAGACCAACGccc                                                                                                                                            >  1:71641/1‑95 (MQ=255)
                                                                 tGCCCAGCCGGTGCGGCGCAGTGAACCACTGTTATCGATATCGAACAGTACGCCTTCCTCGATACCCGTCAGACCAACGCCCTTGCCGCTGAGGTCGAGCAAGAGCGGGTCGACAAACGTGTTCACCGTGGTCATTGCTGCCAACCCGtt                                                                        <  2:567552/150‑1 (MQ=255)
                                                                           gTGCGGCGCAGTGAACCACTGTTATCGATATCGAACAGTACGCCTTCCTCGATACCCGTCAGACCAACGCCCTTGCCGCTGAGGTCGAGCAAGAGCGGGTCGACAAACGTGTTCACCGTGGTCATTGCTGCCAACCCGTTGAGTACCACg                                                              >  1:1102760/1‑150 (MQ=255)
                                                                                              tGTTATCGATATCGAACAGTACGCCTTCCTCGATACCCGTCAGACCAACGCCCTTGCCGCTGAGGTCGAGCAAGAGCGGGTCGACAAACGTGTTCACCGTGGTCATTGCTGCCAACCCGTTGAGTACCACGGCATTGCGCAGGCTGAAAt                                           >  1:982511/1‑150 (MQ=255)
                                                                                               gTTATCGATATCGAACAGTACGCCTTCCTCGATACCCGTCAGACCAACGCCCTTGCCGCTGAGGTCGAGCAAGAGCGGGTCGACAAACGTGTTCACCGTGGTCATTGCTGCCAACCCGTTGAGTACCACGGCATTGCGCAGGCTGAAATc                                          <  1:881121/150‑1 (MQ=255)
                                                                                                 tatcgatatcgaACAGTACGCCTTCCTCGATACCCGTCAGACCAACGCCCTTGCCGCTGAGGTCGAGCAAGAGCGGGTCGACAAACGTGTTCACCGTGGTCATTGCTGCCAACCCGTTGAGTACCACGGCATTGCGCAGGCTGAAATCCg                                        <  2:66403/150‑1 (MQ=255)
                                                                                                      atatcgaACAGTACGCCTTCCTCGATACCCGTCATACCAACGCCCTTGCCGCTGAGGTCGAGCAAGAGCGGGTCGACAAACGTGTTCACCGTGGTCATTGCTGCCAACCCGTTGAGTACCACGGCATTGCGCAGGCTGAAATCCgg                                       >  2:519778/1‑146 (MQ=255)
                                                                                                      atatcgaACAGTACGCCTTCCTCGATACCCGTCATACCAACGCCCTTGCCGCTGAGGTCGAGCAAGAGCGGGTCGACAAACGTGTTCACCGTGGTCATTGCTGCCAACCCGTTGAGTACCACGGCATTGCGCAGGCTGAAATCCgg                                       >  2:519793/1‑146 (MQ=255)
                                                                                                      atatcgaACAGTACGCCTTCCTCGATACCCGTCATACCAACGCCCTTGCCGCTGAGGTCGAGCAAGAGCGGGTCGACAAACGTGTTCACCGTGGTCATTGCTGCCAACCCGTTGAGTACCACGGCATTGCGCAGGCTGAAATCCgg                                       <  1:519778/146‑1 (MQ=255)
                                                                                                      atatcgaACAGTACGCCTTCCTCGATACCCGTCATACCAACGCCCTTGCCGCTGAGGTCGAGCAAGAGCGGGTCGACAAACGTGTTCACCGTGGTCATTGCTGCCAACCCGTTGAGTACCACGGCATTGCGCAGGCTGAAATCCgg                                       <  1:519793/146‑1 (MQ=255)
                                                                                                                       ttCCTCGATACCCGTCAGACCAACGCCCTTGCCGCTGAGGTCGAGCAAGAGCGGGTCGACAAACGTGTTCACCGTGGTCATTGCTGCCAACCCGTTGAGTACCACGGCATTGCGCAGGCTGAAATCCGGTTGTTCATTGCCa                          <  2:626993/142‑1 (MQ=255)
                                                                                                                       ttCCTCGATACCCGTCAGACCAACGCCCTTGCCGCTGAGGTCGAGCAAGAGCGGGTCGACAAACGTGTTCACCGTGGTCATTGCTGCCAACCCGTTGAGTACCACGGCATTGCGCAGGCTGAAATCCGGTTGTTCATTGCCa                          >  1:626993/1‑142 (MQ=255)
                                                                                                                          ctggATACCCGTCAGACCAACGCCCTTGCCGCTGAGGTCGAGCAAGAGCGGGTCGACAAACGTGTTCACCGTGGTCATTGCTGCCAACCCGTTGAGTACCACGGCATTGCGCAGGCTGAAATCCGgtt                                     >  1:1204385/4‑128 (MQ=255)
                                                                                                                          cTCGATACCCGTCAGACCAACGCCCTTGCCGCTGAGGTCGAGCAAGAGCGGGTCGACAAACGTGTTCACCGTGGTCATTGCTGCCAACCCGTTGAGTACCACGGCATTGCGCAGGCTGAAATCCGgtt                                     <  2:1204385/128‑1 (MQ=255)
                                                                                                                                  ccGTCAGACCAACGCCCTTGCCGCTGAGGTCGAGCAAGAGCGGGTCGACAAACGTGTTCACCGTGGTCATTGCTGCCAACCCGTTGAGTACCACGGCATTGCGCAGGCTGAAATCCGGTTGTTCATTGCCATCACGATAGAACTGAGCCa       >  2:548240/1‑150 (MQ=255)
                                                                                                                                   cGTCAGACCAACGCCCTTGCCGCTGAGGTCGAGCAAGAGCGGGTCGACAAACGTGTTCACCGTGGTCATTGCTGCCAACCCGTTGAGTACCACGGCATTGCGCAGGCTGAAATCCgg                                       <  2:93873/117‑1 (MQ=255)
                                                                                                                                   cGTCAGACCAACGCCCTTGCCGCTGAGGTCGAGCAAGAGCGGGTCGACAAACGTGTTCACCGTGGTCATTGCTGCCAACCCGTTGAGTACCACGGCATTGCGCAGGCTGAAATCCgg                                       >  1:93873/1‑117 (MQ=255)
                                                                                                                                    gTCAGACCAACGCCCTTGCCGCTGAGGTCGAGCAAGAGCGGGTCGACAAACGTGTTCACCGTGGTCATTGCTGCCAACCCGTTGAGTACCACGGCATTGCGCAGGCTGAAATCCgg                                       <  2:1106343/116‑1 (MQ=255)
                                                                                                                                    gTCAGACCAACGCCCTTGCCGCTGAGGTCGAGCAAGAGCGGGTCGACAAACGTGTTCACCGTGGTCATTGCTGCCAACCCGTTGAGTACCACGGCATTGCGCAGGCTGAAATCCgg                                       >  1:1106343/1‑116 (MQ=255)
                                                                                                                                    gTCAGACCAACGCCCTTGCCGCTGAGGTCGAGCAAGAGCGGGTCGACAAACGTGTTCACCGTGGTCATTGCTGCCAACCCGTTGAGTACCACGGCATTGCGCAGGCTGAAATCCGGTTGTTCATTGCCATCACGATAGAACTGAGCCAGa     >  2:852992/1‑150 (MQ=255)
                                                                                                                                     tCAGACCAACGCCCTTGCCGCTGAGGTCGAGCAAGAGCGGGTCGACAAACGTGTTCACCGTGGTCATTGCTGCCAACCCGTTGAGTACCACGGCATTGCGCAGGCTGAAATCCGGTTGTTCATTGCCATCACGATAGAACTGAGCCAGAt    <  1:548240/150‑1 (MQ=255)
                                                                                                                                       aGACCAACGCCCTTGCCGCTGAGGTCGAGCAAGAGCGGGTCGACAAACGTGTTCACCGTGGTCATTGCTGCCAACCCGTTGAGTACCACGGCATTGCGCAGGCTGAAATCCGGTTGTTCATTGCCATCACGATAGAACTGAGCCAGATGc  >  1:1042607/1‑150 (MQ=255)
                                                                                                                                        |                                                                                                                                                    
AGCTGGCTGACGTTGCTCACCTGGCCGGTACCGTTGTCATGCACCAGCATGCCGGTCAGCCGGTCTGCCCAGCCGGTGCGGCGCAGTGAACCACTGTTATCGATATCGAACAGTACGCCTTCCTCGATACCCGTCAGACCAACGCCCTTGCCGCTGAGGTCGAGCAAGAGCGGGTCGACAAACGTGTTCACCGTGGTCATTGCTGCCAACCCGTTGAGTACCACGGCATTGCGCAGGCTGAAATCCGGTTGTTCATTGCCATCACGATAGAACTGAGCCAGATGC  >  NC_002947/5602337‑5602621

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑