Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,625,146 C→A 7.6% P222P (CCG→CCT)  PP_4943 ← glycosyl transferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,625,1460CA 7.6% 166.1 / 2.9 53P222P (CCG→CCT) PP_4943glycosyl transferase
Reads supporting (aligned to +/- strand):  ref base C (25/24);  new base A (2/2);  total (27/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.60e-01

AACGCCTGGAAATAACGGCGCGCATCCGGTACCTGGCCAAGAAAGTCGACTTGCCCGGCAATGCCAAGTTCGGCGGCCAACGCCTTGAGCCTGGCCTCCAGACGCCCCTTGCCGAGGATTGCCAGGCGGGCGCCAGCCGGCAGCCCCGGCAGTGCCTGGGCAAAACCGCGCAACAGGGTGGCCTGATCCTTGTCCGGGTGCAGGCGCCCAACATTGCCGACGATCCACGCCTGAGCATCCAGGCCCAAGGCCTGGCGTGCGTCAGCGCGCGGCACCAGGGCTGCTTGCAG  >  NC_002947/5625000‑5625289
                                                                                                                                                  |                                                                                                                                               
aaCGCCTGGAAATAACGGCGCGCATCCGGTACCTGGCCAAGAAAGTCGACTTGCCCGGCAATGCCAAGTTCGGCGGCCAACGCCTTGAGCCTGGCCTCCAGACGCCCCTTGCCGAGGATTGCCAGGCGGGCGCCAGCCGGCAGCCCCGGc                                                                                                                                              >  1:615202/1‑150 (MQ=255)
     cTGGAAATAACGGCGCGCATCCGGTACCTGGCCAAGAAAGTCGACTTGCCCGGCAATGCCAAGTTCGGCGGCCAACGCCTTGAGCCTGGCCTCCAGACGCCCCTTGCCGAGGATTGCCAGGCGGGCGCCAGCCGGCAGCCCCGGCAGTGc                                                                                                                                         >  2:990329/1‑150 (MQ=255)
     cTGGAAATAACGGCGCGCATCCGGTACCTGGCCAAGAAAGTCGACTTGCCCGGCAATGCCAAGTTCGGCGGCCAACGCCTTGAGCCTGGCCTCCAGACGCCCCTTGCCGAGGATTGCCAGGCGGGCGCCAGCCGGCAGCCCCGGCAGTGc                                                                                                                                         >  1:447658/1‑150 (MQ=255)
      tGGAAATAACGGCGCGCATCCGGTACCTGGCCAAGAAAGTCGACTTGCCCGGCAATGCCAAGTTCGGCGGCCAACGCCTTGAGCCTGGCCTCCAGACGCCCCTTGCCGAGGATTGCCAGGCGGGCGCCAGCCGGCAGCCCCGGCAGTGcc                                                                                                                                        >  1:862921/1‑150 (MQ=255)
       ggAAATAACGGCGCGCATCCGGTACCTGGCCAAGAAAGTCGACTTGCCCGGCAATGCCAAGTTCGGCGGCCAACGCCTTGAGCCTGGCCTCCAGACGCCCCTTGCCGAGGATTGCCAGGCGGGCGCCAGCCGGCAGCCCCGGCAGTGCCt                                                                                                                                       >  1:925344/1‑150 (MQ=255)
        gAAATAACGGCGCGCATCCGGTACCTGGCCAAGAAAGTCGACTTGCCCGGCAATGCCAAGTTCGGCGGCCAACGCCTTGAGCCTGGCCTCCAGACGCCCCTTGCCGAGGATTGCCAGGCGGGCGCCAGCCGGCAGCCCCGGCAGTGCCTg                                                                                                                                      <  2:497166/150‑1 (MQ=255)
         aaaTAACGGCGCGCATCCGGTACCTGGCCAAGAAAGTCGACTTGCCCGGCAATGCCAAGTTCGGCGGCCAACGCCTTGAGCCTGGCCTCCAGACGCCCCTTGCCGAGGATTGCCAGGCGGGCGCCAGCCGGCAGCCCCGGCAGTGCCTgg                                                                                                                                     <  2:493776/150‑1 (MQ=255)
          aaTAACGGCGCGCATCCGGTACCTGGCCAAGAAAGTCGACTTGCCCGGCAATGCCAAGTTCGGCGGCCAACGCCTTGAGCCTGGCCTCCAGACGCCCCTTGCCGAGGATTGCCAGGCGGGCGCCAGCCGGCAGCCCCGGCAGTGCCTggg                                                                                                                                    <  2:725198/150‑1 (MQ=255)
               cGGCGCGCATCCGGTACCTGGCCAAGAAAGTCGACTTGCCCGGCAATGCCAAGTTCGGCGGCCAACGCCTTGAGCCTGGCCTCCAGACGCCCCTTGCCGAGGATTGCCAGGCGGGCGCCAGCCGGCAGCCCCGGCAGTGCCTGGGCaaaa                                                                                                                               <  2:576600/150‑1 (MQ=255)
                ggCGCGCATCCGGTACCTGGCCAAGAAAGTCGACTTGCCCGGCAATGCCAAGTTCGGCGGCCAACGCCTTGAGCCTGGCCTCCAGACGCCCCTTGCCGGGGATGGCCAGGCGGGCGCCAGCCGGCAGCCCCGGCAGTGCCTGGGCAAAAc                                                                                                                              >  1:865836/1‑150 (MQ=255)
                ggCGCGCATCCGGTACCTGGCCAAGAAAGTCGACTTGCCCGGCAATGCCAAGTTCGGCGGCCAACGCCTTGAGCCTGGCCTCCAGACGCCCCTTGCCGAGGATTGCCAGGCGGGCGCCAGCCGGCAGCCCAGGCACTGCCGGGGctgccg                                                                                                                              <  2:981346/150‑6 (MQ=255)
                          cGGTACCTGGCCAAGAAAGTCGACTTGCCCGGCAATGCCAAGTTCGGCGGCCAACGCATTGAGCCTGGCCTCCAGACGCCCCTTGCCGAGGATTGCCAGGCGGGCGCCAGCCGGCAGCCCCGGCAGTGCCTGGGCAAAACCGCGCAACAg                                                                                                                    >  1:757932/1‑150 (MQ=255)
                               ccTGGCCAAGAAAGTCGACTTGCCCGGCAATGCCAAGTTCGGCGGCCAACGCCTTGAGCCTGGCCTCCAGACGCCCCTTGCCGAGGATTGCCAGGCGGGCGCCAGCCGGCAGCCCCGGCAGTGCCTGGGCAAAACCGCGCAACAGGGTgg                                                                                                               <  2:925344/150‑1 (MQ=255)
                                cTGGCCAAGAAAGTCGACTTGCCCGGCAATGCCAAGTTCGGCGGCCAACGCCTTGAGCCTGGCCTCCAGACGCCCCTTGCCGAGGATTGCCAGGCGGGCGCCAGCCGGCAGCCCCGGCAGTGCCTGGGCAAAACCGCGCAACAGGGTGGc                                                                                                              >  2:773191/1‑150 (MQ=255)
                                 tGGCCAAGAAAGTCGACTTGCCCGGCAATGCCAAGTTCGGCGGCCAACGCCTTGAGCCTGGCCTCCAGACGCCCCTTGCCGAGGATTGCCAGGCGGGCGCCAGCCGGCAGCCCCGGCAGTGCCTGGGCAAAACCGCGCAACAGGGTGGcc                                                                                                             >  1:564771/1‑150 (MQ=255)
                                 tGGCCAAGAAAGTCGACTTGCCCGGCAATGCCAAGTTCGGCGGCCAACGCCTTGAGCCTGGCCTCCAGACGCCCCTTGCCGAGGATTGCCAGGCGGGCGCCAGCCGGCAGCCCCGGCAGTGCCTGGGCAAAACCGCGCAACAGGGTGGcc                                                                                                             >  2:323150/1‑150 (MQ=255)
                                    ccAAGAAAGTCGACTTGCCCGGCAATGCCAAGTTCGGCGGCCAACGCCTTGAGCCTGGCCTCCAGACGCCCCTTGCCGAGGATTGCCAGGCGGGCGCCAGCCGGCAGCCCCGGCAGTGCCTGGGCAAAACCGCGCAACAGGGTGGCCTGa                                                                                                          <  2:748654/150‑1 (MQ=255)
                                    ccAAGAAAGTCGACTTGCCCGGCAATGCCAAGTTCGGCGGCCAACGCCTTGAGCCTGGCCTCCAGACGCCCCTTGCCGAGGATTGCCAGGCGGGCGCCAGCCGGCAGCCCCGGCAGTGCCTGGGCAAAACCGCGCAACAGGGTGGCCTGa                                                                                                          <  2:109843/150‑1 (MQ=255)
                                        gAAAGTCGACTTGCCCGGCAATGCCAAGTTCGGCGGCCAACGCCTTGAGCCTGGCCTCCAGACGCCCCTTGCCGAGGATTGCCAGGCGGGCGCCAGCCGGCAGCCCCGGCAGTGCCTGGGCAAAACCGCGCAACAGGGTGGc                                                                                                              <  2:365023/142‑1 (MQ=255)
                                        gAAAGTCGACTTGCCCGGCAATGCCAAGTTCGGCGGCCAACGCCTTGAGCCTGGCCTCCAGACGCCCCTTGCCGAGGATTGCCAGGCGGGCGCCAGCCGGCAGCCCCGGCAGTGCCTGGGCAAAACCGCGCAACAGGGTGGc                                                                                                              >  1:365023/1‑142 (MQ=255)
                                           aGTCGACTTGCCCGGCAATGCCAAGTTCGGCGGCCAACGCCTTGAGCCTGGCCTCCAGACGCCCCTTGCCGAGGATCGCCAGGCGGGCGCCAGCCGGCAGCCCCGGCAGTGCCTGGGCAAAACCGCGCAACa                                                                                                                     <  2:1167030/132‑1 (MQ=255)
                                           aGTCGACTTGCCCGGCAATGCCAAGTTCGGCGGCCAACGCCTTGAGCCTGGCCTCCAGACGCCCCTTGCCGAGGATCGCCAGGCGGGCGCCAGCCGGCAGCCCCGGCAGTGCCTGGGCAAAACCGCGCAACa                                                                                                                     >  1:1167030/1‑132 (MQ=255)
                                               gACTTGCCCGGCAATGCCAAGTTCGGCGGCCAACGCCTTGAGCCTGGCCTCCAGACGCCCCTTGCCGAGGATTGCCAGGCGGGCGCCAGCCGGCAGCCCCGGCAGTGCCTGGGCAAAACCGCGCAACAGGGTGGCCTGATCCTTGTCCgg                                                                                               >  2:582904/1‑150 (MQ=255)
                                                    gCCCGGCAATGCCAAGTTCGGCGGCCAACGCCTTGAGCCTGGCCTCCAGACGCCCCTTGCCGAGGATTGCCAGGCGGGCGCCAGCCGGCAGCCCCGGCAGTGCCTGGGCAAAACCGCGCAACAGGGTGGCCTGATCCTTGTCCGGGTGCa                                                                                          <  1:497460/150‑1 (MQ=255)
                                                      ccGGCAATGCCAAGTTCGGCGGCCAACGCCTTGAGCCTGGCCTCCAGACGCCCCTTGCCGAGGATTGCCAGGCGGGCGCCAGCCGGCAGCCCCGGCAGTGCCTGGGCAAAACCGCGCAACAGGGTGGCCTGATCCTTGTCCGGGTGCAgg                                                                                        >  2:929798/1‑150 (MQ=255)
                                                          cAATGCCAAGTTCGGCGGCCAACGCCTTGAGCCTGGCCTCCAGACGCCCCTTGCCGAGGATTGCCAGGCGGGCGCCAGCCGGCAGCCCCGGCAGTGCCTGGGCAAAACCGCGCAACAGGGTGGCCTGATCCTTGTCCGGGTGCAGGCGcc                                                                                    <  1:412358/150‑1 (MQ=255)
                                                               ccAAGTTCGGCGGCCAACGCCTTGAGCCTGGCCTCCAGACGCCCATTGCCGAGGATTGCCAGGCGGGCGCCAGCCGGCAGCCCCGGCAGTGCCTGGGCAAAACCGCGCAACAGGGTGGCCTGATCCTTGTCCGGGTGCAGGCGCCCAACa                                                                               <  2:1040704/150‑1 (MQ=255)
                                                                 aaGTTCGGCGGCCAACGCCTTGAGCCTGGCCTCCAGACGCCCCTTGCCGAGGATTGCCAGGCGGGCGCCAGCCGGCAGCCCCGGCAGTGCCTGGGCAAAACCGCGCAACAGGGTGGCCTGATCCTTGTCCGGGTGCAGGCGCCCAACAtt                                                                             >  1:320512/1‑150 (MQ=255)
                                                                       ggcggcCAACGCCTTGAGCCTGGCCTCCAGACGCCCCTTGCCGAGGATTGCCAGGCGGGCGCCAGCCGGCAGCCCAGGCACTGCCGGGGctgcc                                                                                                                               >  1:82472/1‑90 (MQ=255)
                                                                       ggcggcCAACGCCTTGAGCCTGGCCTCCAGACGCCCCTTGCCGAGGATTGCCAGGCGGGCGCCAGCCGGCAGCCCAGGCACTGCCGGGGctgcc                                                                                                                               <  2:82472/94‑5 (MQ=255)
                                                                         cggcCAACGCCTTGAGCCTGGCCTCCAGACGCCCCTTGCCGAGGATTGCCAGGCGGGCGCCAGCCGGCAGCCCCGGCAGTGCCTGGGCAAAACCGCGCAACAGGGTGGCCTGATCCTTGTCCGGGTGCAGGCGCCCAACATTGCcgacga                                                                     <  2:615202/150‑1 (MQ=255)
                                                                          ggcCAACGCCTTGAGCCTGGCCTCCAGACGCCCCTTGCCGAGGATTGCCAGGCGGGCGCCAGCCGGCAGCCCCGGCAGTGCCTGGGCAAAACCGCGCAACAGGGTGGCCTGATCCTTGTCCGGGTGCAGGCGCCCAACATTGCCGACGAt                                                                    <  1:582904/150‑1 (MQ=255)
                                                                              aaCGCCTTGAGCCTGGCCTCCAGACGCCCCTTGCCGAGGATTGCCAGGCGGGCGCCAGCCGGCAGCCCCGGCAGTGCCTGGGCAAAACCGCGCAACAGGGTGGCCTGATCCTTGTCCGGGTGCAGGCGCCCAACATTGCcga                                                                        <  1:847216/142‑1 (MQ=255)
                                                                              aaCGCCTTGAGCCTGGCCTCCAGACGCCCCTTGCCGAGGATTGCCAGGCGGGCGCCAGCCGGCAGCCCCGGCAGTGCCTGGGCAAAACCGCGCAACAGGGTGGCCTGATCCTTGTCCGGGTGCAGGCGCCCAACATTGCcga                                                                        >  2:847216/1‑142 (MQ=255)
                                                                                   cTTGAGCCTGGCCTCCAGACGCCCCTTGCCGAGGATTGCCAGGCGGGCGCCAGCCGGCAGCCCCGGCAGTGCCTGGGCAAAACCGCGCAACAGGGTGGCCTGATCCTTGTCCGGGTGCAGGCGCCCAACATTGCCGACGATCCACGCCTg                                                           >  2:915209/1‑150 (MQ=255)
                                                                                        gCCTGGCCTCCAGACGCCCCTTGCCGAGGATTGCCAGGCGGGCGCCAGCCGGCAGCCCCGGCAGTGCCTGGGCAAAACCGCGCAACAGGGTGGCCTGATCCTTGTCCGGGTGCAGGCGCCCAACATTGCCGACGATCCACGCCTGAGCAt                                                      >  1:864099/1‑150 (MQ=255)
                                                                                                    gACGCCCCTTGCCGAGGATTGCCAGGCGGGCGCCAGCCGGCAGCCCCGGCAGTGCCTGGGCAAAACCGCGCAACAGGGTGGCCTGATCCTTGTCCGGGt                                                                                             >  1:1105443/1‑99 (MQ=255)
                                                                                                    gACGCCCCTTGCCGAGGATTGCCAGGCGGGCGCCAGCCGGCAGCCCCGGCAGTGCCTGGGCAAAACCGCGCAACAGGGTGGCCTGATCCTTGTCCGGGt                                                                                             <  2:1105443/99‑1 (MQ=255)
                                                                                                          ccTTGCCGAGGATTGCCAGGCGGGCGCCAGCCGGCAGCCCCGGCAGTGCCTGGGCAAAACCGCGCAACAGGGTGGCCTGATCCTTGTCCGGGTGCAGGCGCCCAACATTGCCGACGATCCACGCCTGAGCATCCAGGCCCAAGGCCTTgc                                    <  1:1207130/150‑1 (MQ=255)
                                                                                                          ccTTGCCGAGGATTGCCAGGCGGGCGCCAGCCGGCAGCCCCGGCAGTGCCTGGGCAAAACCGCGCAACAGGGTGGCCTGATCCTTGTCCGGGTGCAGGCGCCCAACATTGCCGACGATCCACGCCTGAGCATCCAGGCCCAAGGCCTGgc                                    >  2:1028735/1‑150 (MQ=255)
                                                                                                              gCCGAGGATTGCCAGGCGGGCGCCAGCCGGCAGCCCCGGCAGTGCCTGGGCAAAACCGCGCAACAGGGTGGCCTGATCCTTGTCCGGGTGCAGGCGCCCAACATTGCCGACGATCCACGCCTGAGCATCCAGGCCCAAGGCCTGgcgtgc                                <  1:323150/150‑1 (MQ=255)
                                                                                                                cGAGGATTGCCAGGCGGGCGCCAGCCGGCAGCCCCGGCAGTGCCTGGGCAAAACCGCGCAACAGGGTGGCCTGATCCTTGTCCGGGTGCAGGCGCCCAACATTGCCGACGATCCACGCCTGAGCATCCAGGCCCAAGGCCTGgcgtgcgt                              >  1:313591/1‑150 (MQ=255)
                                                                                                                  aGGATTGCCAGGCGGGCGCCAGCCGGCAGCCCCGGCAGTGCCTGGGCAAAACCGCGCAACAGGGTGGCCTGATCCTTGTCCGGGTGCAGGCGCCCAACATTGCCGACGATCCACGCCTGAGCATCCAGGCCCAAGGCCTGGCGTGCGTCa                            <  2:757932/150‑1 (MQ=255)
                                                                                                                   ggATTGCCAGGCGGGCGCCAGCCGGCAGCCCCGGGAGTGCCTGGGCAAAACCGCGCAACAGGGTGGCCTGATCCTTGTCCGGGTGCAGGCGCCCAACATTGCCGACGATCCACGCCTGAGCATCCAGGCCCAAGGCCTGGCGTGCGTCAg                           <  2:812673/150‑1 (MQ=255)
                                                                                                                         ccAGGCGGGCGCCAGCCGGCAGCCCCGGCAGTGCCTGGGCAAAACCGCGCAACAGGGTGGCCTGATCCTTGTCCGGGTGCAGGCGCCCAACATTGCCGACGATCCACGCCTGAGCATCCAGGCCCAAGGCCTGgcgt                                  <  1:370391/137‑1 (MQ=255)
                                                                                                                         ccAGGCGGGCGCCAGCCGGCAGCCCCGGCAGTGCCTGGGCAAAACCGCGCAACAGGGTGGCCTGATCCTTGTCCGGGTGCAGGCGCCCAACATTGCCGACGATCCACGCCTGAGCATCCAGGCCCAAGGCCTGgcgt                                  >  2:370391/1‑137 (MQ=255)
                                                                                                                                     cAGCCGGCAGCCCCGGCAGTGCCTGGGCAAAACCGCGCAACAGGGTGGCCTGATCCTTGTCCGGGTGCAGGCGCCCAACATTGCCGACGATCCACGCCTGAGCATCCAGGCCCAAGGCCTGGCGTGCGTCAGCGCGCGGCACCAGGgctg         >  1:863673/1‑150 (MQ=255)
                                                                                                                                        ccGGCAGCCCCGGCAGTGCCTGGGCAAAACCGCGCAACAGGGTGGCCTGATCCtt                                                                                                     <  2:708865/55‑1 (MQ=255)
                                                                                                                                        ccGGCAGCCCCGGCAGTGCCTGGGCAAAACCGCGCAACAGGGTGGCCTGATCCtt                                                                                                     >  1:708865/1‑55 (MQ=255)
                                                                                                                                           gCAGCCCCGGCAGTGCCTGGGCAAAACCGCGCAACAGGGTGGCCTGATCCTTGTCCGGGTGCAGgcgc                                                                                     >  2:186317/1‑68 (MQ=255)
                                                                                                                                           gCAGCCCCGGCAGTGCCTGGGCAAAACCGCGCAACAGGGTGGCCTGATCCTTGTCCGGGTGCAGgcgc                                                                                     <  1:186317/68‑1 (MQ=255)
                                                                                                                                            ttgCCCAGGCACTGCCGGGGCAAAACCGCGCAACAGGGTGGCCTGATCCTTGTCCGGGTGCAGGCGCCCAACATTGCCGACGATCCACGCCTGAGCATCCAGGCCCAAGGCCTGGCGTGCGTCAGCGCGCGGCACCAGGGCTGCTTGCAg  >  2:554721/3‑150 (MQ=255)
                                                                                                                                            cAGCCCCGGCAGTGCCTGGGCAAAACCGCGCAACAGGGTGGCCTGATCCTTGTCCGGGTGCAGGCGCCCAACATTGCCGACGATCCACGCCTGAGCATCCAGGCCCAAGGCCTGGCGTGCGTCAGCGCGCGGCACCAGGGCTGCTTGCAg  <  1:773191/150‑1 (MQ=255)
                                                                                                                                               ccccGGCAGTGCCTGGGCAAAACCGCGCAACAGGGTGGCCTGATCCTTGTCCGGGTGCAGGCGCCCAACATTGCCGACGATCCACGCCTGAGCATCCAGGCCCAAGGCCTGGCGTGCGTCAGCGCGCGGCACCAGGGCTGCTTGCa   <  2:700291/146‑1 (MQ=255)
                                                                                                                                               ccccGGCAGTGCCTGGGCAAAACCGCGCAACAGGGTGGCCTGATCCTTGTCCGGGTGCAGGCGCCCAACATTGCCGACGATCCACGCCTGAGCATCCAGGCCCAAGGCCTGGCGTGCGTCAGCGCGCGGCACCAGGGCTGCTTGCa   >  1:700291/1‑146 (MQ=255)
                                                                                                                                                  |                                                                                                                                               
AACGCCTGGAAATAACGGCGCGCATCCGGTACCTGGCCAAGAAAGTCGACTTGCCCGGCAATGCCAAGTTCGGCGGCCAACGCCTTGAGCCTGGCCTCCAGACGCCCCTTGCCGAGGATTGCCAGGCGGGCGCCAGCCGGCAGCCCCGGCAGTGCCTGGGCAAAACCGCGCAACAGGGTGGCCTGATCCTTGTCCGGGTGCAGGCGCCCAACATTGCCGACGATCCACGCCTGAGCATCCAGGCCCAAGGCCTGGCGTGCGTCAGCGCGCGGCACCAGGGCTGCTTGCAG  >  NC_002947/5625000‑5625289

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑