Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,625,156 T→G 9.5% Q219P (CAG→CCG)  PP_4943 ← glycosyl transferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,625,1560TG 9.5% 133.4 / 8.2 53Q219P (CAG→CCG) PP_4943glycosyl transferase
Reads supporting (aligned to +/- strand):  ref base T (23/25);  new base G (2/3);  total (25/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.08e-01

GGAAATAACGGCGCGCATCCGGTACCTGGCCAAGAAAGTCGACTTGCCCGGCAATGCCAAGTTCGGCGGCCAACGCCTTGAGCCTGGCCTCCAGACGCCCCTTGCCGAGGATTGCCAGGCGGGCGCCAGCCGGCAGCCCCGGCAGTGCCTGGGCAAAACCGCGCAACAGGGTGGCCTGATCCTTGTCCGGGTGCAGGCGCCCAACATTGCCGACGATCCACGCCTGAGCATCCAGGCCCAAGGCCTGGCGTGCGTCAGCGCGCGGCACCAGGGCTGCTTGCAGGGCGTCGATGTCG  >  NC_002947/5625007‑5625302
                                                                                                                                                     |                                                                                                                                                  
ggAAATAACGGCGCGCATCCGGTACCTGGCCAAGAAAGTCGACTTGCCCGGCAATGCCAAGTTCGGCGGCCAACGCCTTGAGCCTGGCCTCCAGACGCCCCTTGCCGAGGATTGCCAGGCGGGCGCCAGCCGGCAGCCCCGGCAGTGCCt                                                                                                                                                    >  1:925344/1‑150 (MQ=255)
 gAAATAACGGCGCGCATCCGGTACCTGGCCAAGAAAGTCGACTTGCCCGGCAATGCCAAGTTCGGCGGCCAACGCCTTGAGCCTGGCCTCCAGACGCCCCTTGCCGAGGATTGCCAGGCGGGCGCCAGCCGGCAGCCCCGGCAGTGCCTg                                                                                                                                                   <  2:497166/150‑1 (MQ=255)
  aaaTAACGGCGCGCATCCGGTACCTGGCCAAGAAAGTCGACTTGCCCGGCAATGCCAAGTTCGGCGGCCAACGCCTTGAGCCTGGCCTCCAGACGCCCCTTGCCGAGGATTGCCAGGCGGGCGCCAGCCGGCAGCCCCGGCAGTGCCTgg                                                                                                                                                  <  2:493776/150‑1 (MQ=255)
   aaTAACGGCGCGCATCCGGTACCTGGCCAAGAAAGTCGACTTGCCCGGCAATGCCAAGTTCGGCGGCCAACGCCTTGAGCCTGGCCTCCAGACGCCCCTTGCCGAGGATTGCCAGGCGGGCGCCAGCCGGCAGCCCCGGCAGTGCCTggg                                                                                                                                                 <  2:725198/150‑1 (MQ=255)
        cGGCGCGCATCCGGTACCTGGCCAAGAAAGTCGACTTGCCCGGCAATGCCAAGTTCGGCGGCCAACGCCTTGAGCCTGGCCTCCAGACGCCCCTTGCCGAGGATTGCCAGGCGGGCGCCAGCCGGCAGCCCCGGCAGTGCCTGGGCaaaa                                                                                                                                            <  2:576600/150‑1 (MQ=255)
         ggCGCGCATCCGGTACCTGGCCAAGAAAGTCGACTTGCCCGGCAATGCCAAGTTCGGCGGCCAACGCCTTGAGCCTGGCCTCCAGACGCCCCTTGCCGGGGATGGCCAGGCGGGCGCCAGCCGGCAGCCCCGGCAGTGCCTGGGCAAAAc                                                                                                                                           >  1:865836/1‑150 (MQ=255)
         ggCGCGCATCCGGTACCTGGCCAAGAAAGTCGACTTGCCCGGCAATGCCAAGTTCGGCGGCCAACGCCTTGAGCCTGGCCTCCAGACGCCCCTTGCCGAGGATTGCCAGGCGGGCGCCAGCCGGCAGCCCAGGCACTGCCGGGGctgccg                                                                                                                                           <  2:981346/150‑6 (MQ=255)
                   cGGTACCTGGCCAAGAAAGTCGACTTGCCCGGCAATGCCAAGTTCGGCGGCCAACGCATTGAGCCTGGCCTCCAGACGCCCCTTGCCGAGGATTGCCAGGCGGGCGCCAGCCGGCAGCCCCGGCAGTGCCTGGGCAAAACCGCGCAACAg                                                                                                                                 >  1:757932/1‑150 (MQ=255)
                        ccTGGCCAAGAAAGTCGACTTGCCCGGCAATGCCAAGTTCGGCGGCCAACGCCTTGAGCCTGGCCTCCAGACGCCCCTTGCCGAGGATTGCCAGGCGGGCGCCAGCCGGCAGCCCCGGCAGTGCCTGGGCAAAACCGCGCAACAGGGTgg                                                                                                                            <  2:925344/150‑1 (MQ=255)
                         cTGGCCAAGAAAGTCGACTTGCCCGGCAATGCCAAGTTCGGCGGCCAACGCCTTGAGCCTGGCCTCCAGACGCCCCTTGCCGAGGATTGCCAGGCGGGCGCCAGCCGGCAGCCCCGGCAGTGCCTGGGCAAAACCGCGCAACAGGGTGGc                                                                                                                           >  2:773191/1‑150 (MQ=255)
                          tGGCCAAGAAAGTCGACTTGCCCGGCAATGCCAAGTTCGGCGGCCAACGCCTTGAGCCTGGCCTCCAGACGCCCCTTGCCGAGGATTGCCAGGCGGGCGCCAGCCGGCAGCCCCGGCAGTGCCTGGGCAAAACCGCGCAACAGGGTGGcc                                                                                                                          >  2:323150/1‑150 (MQ=255)
                          tGGCCAAGAAAGTCGACTTGCCCGGCAATGCCAAGTTCGGCGGCCAACGCCTTGAGCCTGGCCTCCAGACGCCCCTTGCCGAGGATTGCCAGGCGGGCGCCAGCCGGCAGCCCCGGCAGTGCCTGGGCAAAACCGCGCAACAGGGTGGcc                                                                                                                          >  1:564771/1‑150 (MQ=255)
                             ccAAGAAAGTCGACTTGCCCGGCAATGCCAAGTTCGGCGGCCAACGCCTTGAGCCTGGCCTCCAGACGCCCCTTGCCGAGGATTGCCAGGCGGGCGCCAGCCGGCAGCCCCGGCAGTGCCTGGGCAAAACCGCGCAACAGGGTGGCCTGa                                                                                                                       <  2:748654/150‑1 (MQ=255)
                             ccAAGAAAGTCGACTTGCCCGGCAATGCCAAGTTCGGCGGCCAACGCCTTGAGCCTGGCCTCCAGACGCCCCTTGCCGAGGATTGCCAGGCGGGCGCCAGCCGGCAGCCCCGGCAGTGCCTGGGCAAAACCGCGCAACAGGGTGGCCTGa                                                                                                                       <  2:109843/150‑1 (MQ=255)
                                 gAAAGTCGACTTGCCCGGCAATGCCAAGTTCGGCGGCCAACGCCTTGAGCCTGGCCTCCAGACGCCCCTTGCCGAGGATTGCCAGGCGGGCGCCAGCCGGCAGCCCCGGCAGTGCCTGGGCAAAACCGCGCAACAGGGTGGc                                                                                                                           >  1:365023/1‑142 (MQ=255)
                                 gAAAGTCGACTTGCCCGGCAATGCCAAGTTCGGCGGCCAACGCCTTGAGCCTGGCCTCCAGACGCCCCTTGCCGAGGATTGCCAGGCGGGCGCCAGCCGGCAGCCCCGGCAGTGCCTGGGCAAAACCGCGCAACAGGGTGGc                                                                                                                           <  2:365023/142‑1 (MQ=255)
                                    aGTCGACTTGCCCGGCAATGCCAAGTTCGGCGGCCAACGCCTTGAGCCTGGCCTCCAGACGCCCCTTGCCGAGGATCGCCAGGCGGGCGCCAGCCGGCAGCCCCGGCAGTGCCTGGGCAAAACCGCGCAACa                                                                                                                                  <  2:1167030/132‑1 (MQ=255)
                                    aGTCGACTTGCCCGGCAATGCCAAGTTCGGCGGCCAACGCCTTGAGCCTGGCCTCCAGACGCCCCTTGCCGAGGATCGCCAGGCGGGCGCCAGCCGGCAGCCCCGGCAGTGCCTGGGCAAAACCGCGCAACa                                                                                                                                  >  1:1167030/1‑132 (MQ=255)
                                        gACTTGCCCGGCAATGCCAAGTTCGGCGGCCAACGCCTTGAGCCTGGCCTCCAGACGCCCCTTGCCGAGGATTGCCAGGCGGGCGCCAGCCGGCAGCCCCGGCAGTGCCTGGGCAAAACCGCGCAACAGGGTGGCCTGATCCTTGTCCgg                                                                                                            >  2:582904/1‑150 (MQ=255)
                                             gCCCGGCAATGCCAAGTTCGGCGGCCAACGCCTTGAGCCTGGCCTCCAGACGCCCCTTGCCGAGGATTGCCAGGCGGGCGCCAGCCGGCAGCCCCGGCAGTGCCTGGGCAAAACCGCGCAACAGGGTGGCCTGATCCTTGTCCGGGTGCa                                                                                                       <  1:497460/150‑1 (MQ=255)
                                               ccGGCAATGCCAAGTTCGGCGGCCAACGCCTTGAGCCTGGCCTCCAGACGCCCCTTGCCGAGGATTGCCAGGCGGGCGCCAGCCGGCAGCCCCGGCAGTGCCTGGGCAAAACCGCGCAACAGGGTGGCCTGATCCTTGTCCGGGTGCAgg                                                                                                     >  2:929798/1‑150 (MQ=255)
                                                   cAATGCCAAGTTCGGCGGCCAACGCCTTGAGCCTGGCCTCCAGACGCCCCTTGCCGAGGATTGCCAGGCGGGCGCCAGCCGGCAGCCCCGGCAGTGCCTGGGCAAAACCGCGCAACAGGGTGGCCTGATCCTTGTCCGGGTGCAGGCGcc                                                                                                 <  1:412358/150‑1 (MQ=255)
                                                        ccAAGTTCGGCGGCCAACGCCTTGAGCCTGGCCTCCAGACGCCCATTGCCGAGGATTGCCAGGCGGGCGCCAGCCGGCAGCCCCGGCAGTGCCTGGGCAAAACCGCGCAACAGGGTGGCCTGATCCTTGTCCGGGTGCAGGCGCCCAACa                                                                                            <  2:1040704/150‑1 (MQ=255)
                                                          aaGTTCGGCGGCCAACGCCTTGAGCCTGGCCTCCAGACGCCCCTTGCCGAGGATTGCCAGGCGGGCGCCAGCCGGCAGCCCCGGCAGTGCCTGGGCAAAACCGCGCAACAGGGTGGCCTGATCCTTGTCCGGGTGCAGGCGCCCAACAtt                                                                                          >  1:320512/1‑150 (MQ=255)
                                                                ggcggcCAACGCCTTGAGCCTGGCCTCCAGACGCCCCTTGCCGAGGATTGCCAGGCGGGCGCCAGCCGGCAGCCCAGGCACTGCCGGGGctgcc                                                                                                                                            >  1:82472/1‑90 (MQ=255)
                                                                ggcggcCAACGCCTTGAGCCTGGCCTCCAGACGCCCCTTGCCGAGGATTGCCAGGCGGGCGCCAGCCGGCAGCCCAGGCACTGCCGGGGctgcc                                                                                                                                            <  2:82472/94‑5 (MQ=255)
                                                                  cggcCAACGCCTTGAGCCTGGCCTCCAGACGCCCCTTGCCGAGGATTGCCAGGCGGGCGCCAGCCGGCAGCCCCGGCAGTGCCTGGGCAAAACCGCGCAACAGGGTGGCCTGATCCTTGTCCGGGTGCAGGCGCCCAACATTGCcgacga                                                                                  <  2:615202/150‑1 (MQ=255)
                                                                   ggcCAACGCCTTGAGCCTGGCCTCCAGACGCCCCTTGCCGAGGATTGCCAGGCGGGCGCCAGCCGGCAGCCCCGGCAGTGCCTGGGCAAAACCGCGCAACAGGGTGGCCTGATCCTTGTCCGGGTGCAGGCGCCCAACATTGCCGACGAt                                                                                 <  1:582904/150‑1 (MQ=255)
                                                                       aaCGCCTTGAGCCTGGCCTCCAGACGCCCCTTGCCGAGGATTGCCAGGCGGGCGCCAGCCGGCAGCCCCGGCAGTGCCTGGGCAAAACCGCGCAACAGGGTGGCCTGATCCTTGTCCGGGTGCAGGCGCCCAACATTGCcga                                                                                     <  1:847216/142‑1 (MQ=255)
                                                                       aaCGCCTTGAGCCTGGCCTCCAGACGCCCCTTGCCGAGGATTGCCAGGCGGGCGCCAGCCGGCAGCCCCGGCAGTGCCTGGGCAAAACCGCGCAACAGGGTGGCCTGATCCTTGTCCGGGTGCAGGCGCCCAACATTGCcga                                                                                     >  2:847216/1‑142 (MQ=255)
                                                                            cTTGAGCCTGGCCTCCAGACGCCCCTTGCCGAGGATTGCCAGGCGGGCGCCAGCCGGCAGCCCCGGCAGTGCCTGGGCAAAACCGCGCAACAGGGTGGCCTGATCCTTGTCCGGGTGCAGGCGCCCAACATTGCCGACGATCCACGCCTg                                                                        >  2:915209/1‑150 (MQ=255)
                                                                                 gCCTGGCCTCCAGACGCCCCTTGCCGAGGATTGCCAGGCGGGCGCCAGCCGGCAGCCCCGGCAGTGCCTGGGCAAAACCGCGCAACAGGGTGGCCTGATCCTTGTCCGGGTGCAGGCGCCCAACATTGCCGACGATCCACGCCTGAGCAt                                                                   >  1:864099/1‑150 (MQ=255)
                                                                                             gACGCCCCTTGCCGAGGATTGCCAGGCGGGCGCCAGCCGGCAGCCCCGGCAGTGCCTGGGCAAAACCGCGCAACAGGGTGGCCTGATCCTTGTCCGGGt                                                                                                          >  1:1105443/1‑99 (MQ=255)
                                                                                             gACGCCCCTTGCCGAGGATTGCCAGGCGGGCGCCAGCCGGCAGCCCCGGCAGTGCCTGGGCAAAACCGCGCAACAGGGTGGCCTGATCCTTGTCCGGGt                                                                                                          <  2:1105443/99‑1 (MQ=255)
                                                                                                   ccTTGCCGAGGATTGCCAGGCGGGCGCCAGCCGGCAGCCCCGGCAGTGCCTGGGCAAAACCGCGCAACAGGGTGGCCTGATCCTTGTCCGGGTGCAGGCGCCCAACATTGCCGACGATCCACGCCTGAGCATCCAGGCCCAAGGCCTTgc                                                 <  1:1207130/150‑1 (MQ=255)
                                                                                                   ccTTGCCGAGGATTGCCAGGCGGGCGCCAGCCGGCAGCCCCGGCAGTGCCTGGGCAAAACCGCGCAACAGGGTGGCCTGATCCTTGTCCGGGTGCAGGCGCCCAACATTGCCGACGATCCACGCCTGAGCATCCAGGCCCAAGGCCTGgc                                                 >  2:1028735/1‑150 (MQ=255)
                                                                                                       gCCGAGGATTGCCAGGCGGGCGCCAGCCGGCAGCCCCGGCAGTGCCTGGGCAAAACCGCGCAACAGGGTGGCCTGATCCTTGTCCGGGTGCAGGCGCCCAACATTGCCGACGATCCACGCCTGAGCATCCAGGCCCAAGGCCTGgcgtgc                                             <  1:323150/150‑1 (MQ=255)
                                                                                                         cGAGGATTGCCAGGCGGGCGCCAGCCGGCAGCCCCGGCAGTGCCTGGGCAAAACCGCGCAACAGGGTGGCCTGATCCTTGTCCGGGTGCAGGCGCCCAACATTGCCGACGATCCACGCCTGAGCATCCAGGCCCAAGGCCTGgcgtgcgt                                           >  1:313591/1‑150 (MQ=255)
                                                                                                           aGGATTGCCAGGCGGGCGCCAGCCGGCAGCCCCGGCAGTGCCTGGGCAAAACCGCGCAACAGGGTGGCCTGATCCTTGTCCGGGTGCAGGCGCCCAACATTGCCGACGATCCACGCCTGAGCATCCAGGCCCAAGGCCTGGCGTGCGTCa                                         <  2:757932/150‑1 (MQ=255)
                                                                                                            ggATTGCCAGGCGGGCGCCAGCCGGCAGCCCCGGGAGTGCCTGGGCAAAACCGCGCAACAGGGTGGCCTGATCCTTGTCCGGGTGCAGGCGCCCAACATTGCCGACGATCCACGCCTGAGCATCCAGGCCCAAGGCCTGGCGTGCGTCAg                                        <  2:812673/150‑1 (MQ=255)
                                                                                                                  ccAGGCGGGCGCCAGCCGGCAGCCCCGGCAGTGCCTGGGCAAAACCGCGCAACAGGGTGGCCTGATCCTTGTCCGGGTGCAGGCGCCCAACATTGCCGACGATCCACGCCTGAGCATCCAGGCCCAAGGCCTGgcgt                                               >  2:370391/1‑137 (MQ=255)
                                                                                                                  ccAGGCGGGCGCCAGCCGGCAGCCCCGGCAGTGCCTGGGCAAAACCGCGCAACAGGGTGGCCTGATCCTTGTCCGGGTGCAGGCGCCCAACATTGCCGACGATCCACGCCTGAGCATCCAGGCCCAAGGCCTGgcgt                                               <  1:370391/137‑1 (MQ=255)
                                                                                                                              cAGCCGGCAGCCCCGGCAGTGCCTGGGCAAAACCGCGCAACAGGGTGGCCTGATCCTTGTCCGGGTGCAGGCGCCCAACATTGCCGACGATCCACGCCTGAGCATCCAGGCCCAAGGCCTGGCGTGCGTCAGCGCGCGGCACCAGGgctg                      >  1:863673/1‑150 (MQ=255)
                                                                                                                                 ccGGCAGCCCCGGCAGTGCCTGGGCAAAACCGCGCAACAGGGTGGCCTGATCCtt                                                                                                                  <  2:708865/55‑1 (MQ=255)
                                                                                                                                 ccGGCAGCCCCGGCAGTGCCTGGGCAAAACCGCGCAACAGGGTGGCCTGATCCtt                                                                                                                  >  1:708865/1‑55 (MQ=255)
                                                                                                                                    gCAGCCCCGGCAGTGCCTGGGCAAAACCGCGCAACAGGGTGGCCTGATCCTTGTCCGGGTGCAGgcgc                                                                                                  <  1:186317/68‑1 (MQ=255)
                                                                                                                                    gCAGCCCCGGCAGTGCCTGGGCAAAACCGCGCAACAGGGTGGCCTGATCCTTGTCCGGGTGCAGgcgc                                                                                                  >  2:186317/1‑68 (MQ=255)
                                                                                                                                     ttgCCCAGGCACTGCCGGGGCAAAACCGCGCAACAGGGTGGCCTGATCCTTGTCCGGGTGCAGGCGCCCAACATTGCCGACGATCCACGCCTGAGCATCCAGGCCCAAGGCCTGGCGTGCGTCAGCGCGCGGCACCAGGGCTGCTTGCAg               >  2:554721/3‑150 (MQ=255)
                                                                                                                                     cAGCCCCGGCAGTGCCTGGGCAAAACCGCGCAACAGGGTGGCCTGATCCTTGTCCGGGTGCAGGCGCCCAACATTGCCGACGATCCACGCCTGAGCATCCAGGCCCAAGGCCTGGCGTGCGTCAGCGCGCGGCACCAGGGCTGCTTGCAg               <  1:773191/150‑1 (MQ=255)
                                                                                                                                        ccccGGCAGTGCCTGGGCAAAACCGCGCAACAGGGTGGCCTGATCCTTGTCCGGGTGCAGGCGCCCAACATTGCCGACGATCCACGCCTGAGCATCCAGGCCCAAGGCCTGGCGTGCGTCAGCGCGCGGCACCAGGGCTGCTTGCa                <  2:700291/146‑1 (MQ=255)
                                                                                                                                        ccccGGCAGTGCCTGGGCAAAACCGCGCAACAGGGTGGCCTGATCCTTGTCCGGGTGCAGGCGCCCAACATTGCCGACGATCCACGCCTGAGCATCCAGGCCCAAGGCCTGGCGTGCGTCAGCGCGCGGCACCAGGGCTGCTTGCa                >  1:700291/1‑146 (MQ=255)
                                                                                                                                             gCAGTGCCTGGGCAAAACCGCGCAACAGGGTGGCCTGATCCTTGTCCGGGTGCAGGCGCCCAACATTGCCGACGATCCACGCCTGAGCATCCAGGCCCAAGGCCTGGCGTGCGTCAGCGCGCGGCACCAGGGCTGCTTGCAGGGCgtcga       >  1:306758/1‑150 (MQ=255)
                                                                                                                                                ctGCCGGGGCAAAACCGCGCAACAGGGTGGCCTGATCCTTGTCCGGGTGCAGGCGCCCAACATTGCCGACGATCCACGCCTGAGCATCCAGGCCCAAGGCCTGGCGTGCGTCAGCGCGCGGCACCAGGGCTGCTTGCAGGGCgtcgatgt    <  1:554721/149‑1 (MQ=255)
                                                                                                                                                  gCCTGGGCAAAACCGCGCAACAGGGTGGCCTGATCCTTGTCCGGGTGCAGGCGCCCAACATTGCCGACGATCCACGCCTGAGCATCCAGGCCCAAGGCCTGGCGTGCGTCAGCGCGCGGCACCAGGGCTGCTTGCAGGGCgtcgatgtcg  >  2:1092830/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                  
GGAAATAACGGCGCGCATCCGGTACCTGGCCAAGAAAGTCGACTTGCCCGGCAATGCCAAGTTCGGCGGCCAACGCCTTGAGCCTGGCCTCCAGACGCCCCTTGCCGAGGATTGCCAGGCGGGCGCCAGCCGGCAGCCCCGGCAGTGCCTGGGCAAAACCGCGCAACAGGGTGGCCTGATCCTTGTCCGGGTGCAGGCGCCCAACATTGCCGACGATCCACGCCTGAGCATCCAGGCCCAAGGCCTGGCGTGCGTCAGCGCGCGGCACCAGGGCTGCTTGCAGGGCGTCGATGTCG  >  NC_002947/5625007‑5625302

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑