Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_002947 1,443,379 C→A A272S (GCC→TCC)  PP_1262 ← LysR family transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,443,3790CA100.0% 143.6 / NA 44A272S (GCC→TCC) PP_1262LysR family transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (24/20);  total (24/20)

AGCAGCTTTTTAGTTGGCGATCAGCACCTGATGGGTTTTCAGGTCTGCCTCATGCGCGGCAAGAATCTCTGGCAGCGAGTTGCGCAGGTACTCGACCCAGGTCTTGATCTTGGCATCCAGATACTGCCGCGATGGATAGATGGCATACAGGTTCAACTCCTGCAGGCGATACTCCGGCAGTA‑CCCGTACCAGGCTACCGTCCCGCAGGCCCTCGATAGCCGAATAGATCGGCAGCACGCCTACGCCCATGCCACTGCGGATCGCAGTCTTCATGGCATCGGCGGT  >  NC_002947/1443236‑1443520
                                                                                                                                               |                                                                                                                                              
agcagcTTTTTAGTTGGCGATCAGCACCTGATGGGTTTTCAGGTCTGCCTCATGCGCGGCAAGAATCTCTGGCAGCGAGTTGCGCAGGTACTCGACCCAGGTCTTGATCTTGGCATCCAGATACTGCCGCGATGGATAGATGGAATACAg                                                                                                                                          <  1:1239003/150‑1 (MQ=255)
      tttttAGTTGGCGATCAGCACCTGATGGGTTTTCAGGTCTGCCTCATGCGCGGCAAGAATCTCTGGCAGCGAGTTGCGCAGGTACTCGACCCAGGTCTTGATCTTGGCATCCAGATACTGCCGCGATGGATAGATGGAATACAGGTTCaa                                                                                                                                    <  1:854184/150‑1 (MQ=255)
      tttttAGTTGGCGATCAGCACCTGATGGGTTTTCAGGTCTGCCTCATGCGCGGCAAGAATCTCTGGCAGCGAGTTGCGCAGGTACTCGACCCAGGTCTTGATCTTGGCATCCAGATACTGCCGCGATGGATAGATGGAATACAGGTTCaa                                                                                                                                    <  1:1130478/150‑1 (MQ=255)
               ggCGATCAGCACCTGATGGGTTTTCAGGTCTGCCTCATGCGCGGCAAGAATCTCTGGCAGCGAGTTGCGCAGGTACTCGACCCAGGTCTTGATCTTGGCATCCAGATACTGCCGCGATGGATAGATGGAATACAGGTTCAACTCCTGCAg                                                                                                                           <  2:164091/150‑1 (MQ=255)
                   aTCAGCACCTGATGGGTTTTCAGGTCTGCCTCATGCGCGGCAAGAATCTCTGGCAGCGAGTTGCGCAGGTACTCGACCCAGGTCTTGATCTTGGCATCCAGATACTGCCGCGATGGATAGATGGAATACAGGTTCAACTCCTGCAGGCGa                                                                                                                       <  1:1062601/150‑1 (MQ=255)
                       gCACCTGATGGGTTTTCAGGTCTGCCTCATGCGCGGCAAGAATCTCTGGCAGCGAGTTGCGCAGGTACTCGACCCAGGTCTTGATCTTGGCATCCAGATACTGCCGCGATGGATAGATGGAATACAGGTTCAACTCCTGCAGGCGATACt                                                                                                                   >  2:788554/1‑150 (MQ=255)
                        cACCTGATGGGTTTTCAGGTCTGCCTCATGCGCGGCAAGAATCTCTGGCAGCGAGTTGCGCAGGTACTCGACCCAGGTCTTGATCTTGGCATCCAGATACTGCCGCGATGGATAGATGGAATACAGGTTCAACTCCTGCAGGCGATACTc                                                                                                                  >  2:478295/1‑150 (MQ=255)
                        cACCTGATGGGTTTTCAGGTCTGCCTCATGCGCGGCAAGAATCTCTGGCAGCGAGTTGCGCAGGTACTCGACCCAGGTCTTGATCTTGGCATCCAGATACTGCCGCGATGGATAGATGGAATACAGGTTCAACTCCTGCAGGCGATACTc                                                                                                                  >  1:253954/1‑150 (MQ=255)
                        cACCTGATGGGTTTTCAGGTCTGCCTCATGCGCGGCAAGAATCTCTGGCAGCGAGTTGCGCAGGTACTCGACCCAGGGCTTGATCTTGGCATCCAGATACTGCCGCTATGGATAGATGGAATACAGGTTCAACTCCGTCAGGCGATACTc                                                                                                                  >  1:619364/1‑150 (MQ=255)
                             gATGGGTTTTCAGGTCTGCCTCATGCGCGGCAAGAATCTCTGGCAGCGAGTTGCGCAGGTACTCGACCCAGGTCTTGATCTTGGCATCCAGATACTGCCGCGATGGATAGATGGAATACAGGTTCAACTCCTGCAGGCGATACTCCGGCa                                                                                                             <  1:453170/150‑1 (MQ=255)
                             gATGGGTTTTCAGGTCTGCCTCATGCGCGGCAAGAATCTCTGGCAGCGAGTTGCGCAGGTACTCGACCCAGGTCTTGATCTTGGCATCCAGATACTGCCGCGATGGATAGATGGAATACAGGTTCAACTCCTGCAGGCGATACTCCGGCa                                                                                                             >  1:1316779/1‑150 (MQ=255)
                                          gTCTGCCTCATGCGCGGCAAGAATCTCTGGCAGCGAGTTGCGCAGGTACTCGACCCAGGTCTTGATCTTGGCATCCAGATACTGCCGCGATGGATAGATGGAATACAGGTTCAACTCCTGCAGGCGATACTCCGGCAGTA‑CCCGTACCAg                                                                                               >  1:1319999/1‑150 (MQ=255)
                                           tCTGCCTCATGCGCGGCAAGAATCTCTGGCAGCGAGTTGCGCAGGTACTCGACCCAGGTCTTGATCTTGGCATCCAGATACTGCCGCGATGGATAGATCGAATACAGGTTCAACTCCTGCAGGCCATACTCCGGCAGTA‑CCCGTACCAgg                                                                                              <  2:394363/150‑1 (MQ=255)
                                                 tCATGCGCGGCAAGAATCTCTGGCAGCGAGTTGCGCAGGTACTCGACCCAGGTCTTGATCTTGGCATCCAGATACTGCCGCGATGGATAGATGGAATACAGGTTCAACTCCTGCAGGCGATACTCCGGCAGTA‑CCCGTACCAGGCTACCg                                                                                        >  2:311252/1‑150 (MQ=255)
                                                      cgcgGCAAGAATCTCTGGCAGCGAGTTGCGCAGGTACTCGACCCAGGTCTTGATCTTGGCATCCAGATACTGCCGCGATGGATAGATGGAATACAGGTTCAACTCCTGCAGGCGATACTCCGGCAGTA‑CCCGTACCAGGCTACCGTccag                                                                                   <  2:850264/150‑3 (MQ=255)
                                                           cAAGAATCTCTGGCAGCGAGTTGCGCAGGTACTCGACCCAGGTCTTGATCTTGGCATCCAGATACTGCCGCGATGGATAGATGGAATACAGGTTCAACTCCTGCAGGCGATACTCCGGCAGTA‑CCCGTACCAGGCTACCGTCCCGCAGGc                                                                              >  2:1234382/1‑150 (MQ=255)
                                                              gAATCTCTGGCAGCGAGTTGCGCAGGTACTCGACCCAGGTCTTGATCTTGGCATCCAGATACTGCCGCGATGGATAGATGGAATACAGGTTCAACTCCTGCAGGCGATACTCCGGCAGTA‑CCCGTACCAGGCTACCGTCCCGCAGGCCCt                                                                           >  1:868447/1‑150 (MQ=255)
                                                                   tctGGCAGCGAGTTGCGCAGGTACTCGACCCAGGTCTTGATCTTGGCATCCAGATACTGCCGCGATGGATAGATGGAATACAGGTTCAACTCCTGCAGGCGATACTCCGGCAGTA‑CCCGTACCAGGCTACCGTCCCGCAGGCCCTCGATa                                                                      <  2:816533/150‑1 (MQ=255)
                                                                        cAGCGAGTTGCGCAGGTACTCGACCCAGGTCTTGATCTTGGCATCCAGATACTGCCGCGATGGATAGATGGAATACAGGTTCAACTCCTGCAGGCGATACTCCGGCAGTA‑CCCGTACCAGGCTACCGTCCCGCAGGCCCTCGATAGCCGa                                                                 <  1:843720/150‑1 (MQ=255)
                                                                         aGCGAGTTGCGCAGGTACTCGACCCAGGTCTTGATCTTGGCATCCAGATACTGCCGCGATGGATAGATGGAATACAGGTTAAACTCCTTCAGGCGATACTCCGGCAGTA‑CCCGTACCAGGCTACCGTCCCGCAGGCCCTCGATAGCCGaa                                                                <  2:666826/150‑1 (MQ=255)
                                                                             ggTTGCGCAGGTACTCGACCCAGGTCTTGCTCTTGCGCTCCAGACACTGCCGCGAGGGATAGATGGAATACAGGTTCAACTCCTGCAGGCGATACTCCGGCAGTA‑CCCGTACCAGGCTACCGTCCCGCAGGCCCTCGATAGCCGAATAGa                                                            <  2:108840/149‑1 (MQ=255)
                                                                                 gcgcAGGTACTCGACCCAGGTCTTGATCTTCGCATACACATGCTGCTGCCATGGATGGATGGAATACAGTATCTACTACTGCAGGCGATACTCCCGCAAGA‑CCCGGACCAGGCTACCGTCCCGCGGGCCCTCGATAGCCGAATAGATCgg                                                        >  1:705736/1‑150 (MQ=255)
                                                                                     aGGTACTCGACCCAGGTCTTGATCTTGGCATCCAGATACTGCCGCGATGGATAGATGGAATACAGGTTCAACTCCTGCAGGCGATACTCCGGCAGTA‑CCCGTACCAGGCTACCGTCCCGCAGGCCCTCGATAGCCGAATAGATCGgcagc                                                    >  2:1240838/1‑150 (MQ=255)
                                                                                      ggTACTCGACCCAGGTCTTGATCTTGGCATCCAGATACTGCCGCGATGGATAGATGGAATACAGGTTCAACTCCTGCAGGCGATACTCCGGCAGTA‑CCCGTACCAGGCTACCGTCCCGCAGGCCCTCGATAGCCGAATAGATCGgcagca                                                   >  2:844317/1‑150 (MQ=255)
                                                                                       gTACTCGACCCAGGTCTTGATCTTGGCATCCAGATACTGCCGCGATGGATAGATGGAATACAGGTTCAACTCCTGCAGGCGATACTCCGGCAGTA‑CCCGTACCAGGCTACCGTCCCGCAGGCCCTCGATAGCCGAATAGATCGGCAGCAc                                                  >  2:1206853/1‑150 (MQ=255)
                                                                                        tACTCGACCCAGGTCTTGATCTTGGCATCCAGATACTGCCGCGATGGATAGATGGAATACAGGTTCAACTCCTGCAGGCGATATTCCGGCAGTA‑CCCGTACCAGGCTACCGTCCCGCAGGCCCTCGATAGCCGAATAGATCGGCAGCACg                                                 <  2:1230056/150‑1 (MQ=255)
                                                                                         aCTCGACCCAGGTCTTGATCTTGGCATCCAGATACTGCCGCGATGGATAGATTGAATACATGTTCAACTCCTGCAGGCTATACTCCGGCAGTA‑CCCGTACCAGGCTACCGTCCCTTATTCCCTCGATAGCCGAATAGATCGGCAGCACGc                                                <  2:933409/150‑1 (MQ=255)
                                                                                             gACCCAGGTCTTGATCTTGGCATCCAGATACTGCCGCGATGGATAGATGGAATACAGGTTCAACTCCTGCAGGCGATACTCCGGCAGTA‑CCCGTACCAGGCTACCGTCCCGCAGGCCCTCGATAGCCGAATAGATCGGCCGCACGCCTAc                                            >  1:734369/1‑150 (MQ=255)
                                                                                             gACCCAGGTCTTGATCTTGGCATCCAGATACTGCCGCGATGGATAGATGGAATACAGGTTCAACTCCTGCAGGCGATACTCCGGCAGTA‑CCCGTACCAGGCTACCGTCCCGCAGGCCCTCGATAGCCGAATAGATCGGCAGCACGCCTAc                                            >  2:1012447/1‑150 (MQ=255)
                                                                                              aCCCAGGTCTTGATCTTGGCATCCAGATACTGCCGCGATGGATAGATGGAATACAGGTTCAACTCCTGCAGGCGATACTCCGGCAGTA‑CCCGTACCAGGCTACCGTCCCGCAGGCCCTCGATAGCCGAATAGATCGGCAGCACGCCTACg                                           >  1:1290318/1‑150 (MQ=255)
                                                                                                 cAGGTCTTGATCTTGGCATCCAGATACTGCCGCGATGGATAGATGGAATACAGGTTCAACTCCTGCAGGCGATACTCCGGCAGTA‑CCCGTACCAGGCTACCGTCCCGCAGGCCCTCGATAGCCGAATAGATCGGCAGCACGCCTACGccc                                        <  2:726628/150‑1 (MQ=255)
                                                                                                   ggTCTTTATCTTGGCATCCAGATACTGCCGCGATGTATAGATGGAATACAGGTTCAACTCCTGCAGGCGATACTCCGGCAGTA‑CCCGTACCAGGCTACCGTCCCGCAGGCCCTCGATAGCCGAATAGATCGGCAGCACGCCTACGCCCAt                                      <  1:217479/150‑1 (MQ=255)
                                                                                                   ggTCTTGATCTTGGCATCCAGATACTGCCGCGATGTTTTGGGGGAATACAGGTTCAACTCCTGCAGGCGATACT‑CGGAGGTAGCCCGTACCAGGCTACCGTCCCGCAGGCCCTCGATAGCCGAATAGATCGGCAGCACGCCTACGCCCAt                                      >  1:785747/1‑150 (MQ=255)
                                                                                                   ggTCTTGATCTTGGCATCCAGATACTGCCGCGATGGATAGATGGAATACAGGTTCAACTCCTGCAGGCGATACTCCGGCAGTA‑CCCGTACCAGGCTACCGTCCCGCAGGCCCTCGATAGCCGAATAGATCGGCAGCACGCCTACGCCCAt                                      <  1:788554/150‑1 (MQ=255)
                                                                                                      cTTGATCTTGGCATCCAGATACTGCCGCGATGGATAGATGGAATACAGGTTCAACTCCTGCAGGCGATACTCCGGCAGTA‑CCCGTACCAGGCTACCGTCCCGCAGGCCCTCGATAGCCGAATAGATCGGCAGCACGCCTACGCCCATGcc                                   >  2:272362/1‑150 (MQ=255)
                                                                                                         gATCTTGGCATCCAGATACTGCCGCGATGGATAGATGGAATACAGGTTCAACTCCTGCAGGCGATACTCCGGCAGTA‑CCCGTACCAGGCTACCGTCCCGCAGGCCCTCGATAGCCGAATAGATCGGCAGCACGCCTACGCCCCTGCCACt                                >  1:359669/1‑150 (MQ=255)
                                                                                                            cTTGGCATCCAGATACTGCCGCGATGGATAGATGGAATACAGGTTCAACTCCTGCAGGCGATACTCCGGCAGTA‑CCCGTACCAGGCTACCGTCCCGCAGGCCCTCGATAGCCGAATAGATCGGCAGCACGCCTACGCCCa                                       >  2:1312257/1‑140 (MQ=255)
                                                                                                            cTTGGCATCCAGATACTGCCGCGATGGATAGATGGAATACAGGTTCAACTCCTGCAGGCGATACTCCGGCAGTA‑CCCGTACCAGGCTACCGTCCCGCAGGCCCTCGATAGCCGAATAGATCGGCAGCACGCCTACGCCCa                                       <  1:1312257/140‑1 (MQ=255)
                                                                                                               ggCATCCAGATACTGCCGCGATGGATAGATGGAATACAGGTTCAACTCCTGCAGGCGATACTCCGGCAGTA‑CCCGTACCAGGCTACCGTCCCGCAGGCCCTCGATAGCCGAATAGATCGGCAGCACGCCTACGCCCATGCCACTGCGGAt                          <  1:301636/150‑1 (MQ=255)
                                                                                                                gCATCCAGATACTGCCGCGATGGATAGATGGAATACAGGTTCAACTCCTGCAGGCGATACTCCAGCAGTA‑CCCGTACCAGGCTACCGTCCAGCAGGCCCTCGATAGCCGAATAGACCTGCAGCACGCCTACCCACATGCCACTGCGGATc                         >  2:596756/1‑150 (MQ=255)
                                                                                                                   tCCAGATACTGCCGCGATGGATAGATGGAACACAGGTTCAACTCCTGCAGGCGATACTCCGGCAGTA‑CCCGTACCAGGCTACCGTCCCGCAGGCCCTCGATAGCCGAATAGATCGGCAGCACGCCTACGCCCATGCCACTGCGGATCGCa                      >  1:1073045/1‑150 (MQ=255)
                                                                                                                              ccGCGATGGATAGATGGAATACAGTTTCAACTCCTGCAGGCGATACTCCGGCAGTA‑CCCGTACCAGGCTACCGTCCCGCAGGCCCTCGATAGCCGAATAGATCGGCAGCACGCCTACGCCCATGCCACTGCGGATCGCAGTCTTCATGGc           >  2:1126007/1‑150 (MQ=255)
                                                                                                                                   aTGGATAGATGGAATACAGGTTCAACTCCTGCAGGCGATACTCCGGCAGTA‑CCCGTACCAGGCTACCGTCCCGCAGGCCCTCGATAGCCGAATAGATCGGCAGCACGCCTACGCCCATGCCACTGCGGATCGCAGTCTTCATGGCATcgg      <  2:605023/150‑1 (MQ=255)
                                                                                                                                       aTAGATGGAATACAGGTTCAACTCCTGCAGGCGATACTCCGGCAGTA‑CCCGTACCAGGCTACCGTCCCGCAGGCCCTCGATAGCCGAATAGATCGGCAGCACGCCTACGCCCATGCCACTGCGGATCGCAGTCTTCATGGCATCGGCGgt  >  1:1052331/1‑150 (MQ=255)
                                                                                                                                               |                                                                                                                                              
AGCAGCTTTTTAGTTGGCGATCAGCACCTGATGGGTTTTCAGGTCTGCCTCATGCGCGGCAAGAATCTCTGGCAGCGAGTTGCGCAGGTACTCGACCCAGGTCTTGATCTTGGCATCCAGATACTGCCGCGATGGATAGATGGCATACAGGTTCAACTCCTGCAGGCGATACTCCGGCAGTA‑CCCGTACCAGGCTACCGTCCCGCAGGCCCTCGATAGCCGAATAGATCGGCAGCACGCCTACGCCCATGCCACTGCGGATCGCAGTCTTCATGGCATCGGCGGT  >  NC_002947/1443236‑1443520

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑