Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_002947 1,851,755 A→G I267V (ATC→GTC)  relA → ATP:GTP 3'‑pyrophosphotransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,851,7550AG97.6% 132.4 / NA 41I267V (ATC→GTC) relAATP:GTP 3'‑pyrophosphotransferase
Reads supporting (aligned to +/- strand):  ref base A (1/0);  new base G (22/18);  total (23/18)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TACAAGCAGATCGCCAAGCTGTTGCATGAGCGGCGGCTGGACCGCGAGCGCTTCATCAGCGACGTGATGAACCAGCTGCAGAACGAGTTGCTCGCCACTGGCGTGAAGGCCGACATCAGCGGCCGGGCGAAACATATCTATTCGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTACGACGTGCGTGCGGTGCGCGTGCTGGTGCCGGAAATCCGCGACTGCTACACCGCGCTGGGCATCGTGCACACCTTGTGGCGGC  >  NC_002947/1851620‑1851893
                                                                                                                                       |                                                                                                                                          
tACAAGCAGATCGCCAAGCTGTTGCATGAGCGGCGGCTGGACCGCGCGCGCTTCATCAGCGACGTGATGAACCAGCTGCAGAACGAGTTGCTCGCCACTGGCGTGAAGGCCGACATCAGCGGCCGGGCGAAACATGTCTATTCGATCTgg                                                                                                                              >  2:1194311/1‑150 (MQ=255)
tACAAGCAGATCGCCAAGCTGTTGCATGAGCGGCGGCTGGACCGCGAGCGCTTCATCAGCGACGTGATGAACCAGCTGCAGAACGAGTTGCTCGCCACTGGCGTGAAGGCCGACATCAGCGGCCGGGCGAAACATGTCTATTCGATCCgg                                                                                                                              <  1:1334269/150‑1 (MQ=255)
  cAAGCAGATTGTCAAGCTGTTGCATGAGCGGCGGCTGGACCGCGAGCGCTTCATCAGCGACGTGATGAACCAGCTGCAGAACGAGTTGCTCGCCACTGGCGTGAAGGCCGACATCAGCGGCCGGGCGAAACATGTCTATTCGATCTGgcg                                                                                                                            <  2:7220/150‑1 (MQ=255)
   aaGCAGATCGCCAAGCTGTTGCATGAGCGGCGGCTGGACCGCGAGCGCTTCATCAGCGACGTGATGAACCAGCTGCAGAACGAGTTGCTCGCCACTGGCGTGAAGGCCGACATCAGCGGCCGGGCGAAACATGTCTATTCGATCTGgcgc                                                                                                                           >  2:910204/1‑150 (MQ=255)
   aaGCAGATCGCCAAGCTGTTGCATGAGCGGCGGCTGGACCGCGAGCCCTTCATCAGCGACGTGATGAACCAGCTGCAGAACGAGTTGCTCGCCACTGGCGTGAAGGCAGACATCAGCGGCCGGGCGAAACATGTCTATTCGATCTGgcgc                                                                                                                           >  1:444128/1‑150 (MQ=255)
   aaGCAGATCGCCAAGCTGTTGCATGACCGTGGGCTGGACCGCGAGCGCTTCATCAGCGACGTGATGAACCAGCTGCAGAACGAGTTGCTCGCCACTGGCGTGAAGGCCGACATCAGCGGCCGGGCGAAACATGTCTATTCGATCTGgcgc                                                                                                                           >  1:1044771/1‑150 (MQ=255)
        gATCGCCAAGCTGTTGCATGAGCGGCGGCTGGACCGCGAGCGCTTCATCAGCGACGTGATGAACCAGCTGCAGAACGAGTTGCTCGCCACTGGCGTGAAGGCCGACATCAGCGGCCGGGCGAAACATGTCTATTCGATCTGGCGCAAGAt                                                                                                                      >  1:607630/1‑150 (MQ=255)
           ggcaaaGCTGTTGCATGAGCGGCGGCTGGACCGCGAGCGCTTCATCAGCGACGTGATGAACCAGCTGCAGAACGAGTTGCTCGCCAAGGGCTTGAAGGCCGACATCAGCGGCCGGGCGAAACATGTCTATTCGATCTGGCGCAAGATGCa                                                                                                                   <  2:526832/146‑1 (MQ=255)
           cGCCAAGCTGTTGCATGAGCGGCGGCTGGACCGCGAGCGCTTCATCAGCGACGTGATGAACCAGCTGCAGAACGAGTTGCTCGCCACTGGCGTGAAGGCCGACATCAGCGGCCGGGCGAAACATGTCTATTCGATCTGGCGCAAGATGCa                                                                                                                   <  1:910204/150‑1 (MQ=255)
            gCCAAGCTGTTGCATGAGCGGCGGCTGGACCGCGAGCGCTTCATCAGCGACGTGATGAACCAGCTGCAGAACGAGTTGCTCGCCACTGGCGTGTAGGCCGACATCAGCGGCCGGGCGAAACATGTCTATTCGATCTGGCGCAAGATGCAg                                                                                                                  >  1:358683/1‑150 (MQ=255)
             ccAAGCTGTTGCATGAGCGGCGGCTGGACCGCGAGCGCTTCATCAGCGACGTGATGAACCAGCTGCAGAACGAGTTGCTCGCCACTGGCGTGAAGGCCGACATCAGCGGCCGGGCGAAACATGTCTATTCGATCTGGCGCAAGATGCAgc                                                                                                                 >  1:77557/1‑150 (MQ=255)
                    gTTGCATGAGCGGCGGCTGGACCGCGAGCGCTTCATCAGCGACGTGATGAACCAGCTGCAGAACGAGTTGCTCGCCACTGGCGTGAAGGCCGACATCAGCGGCCGGGCGAAACATGTCTATTCGATCTGGCGCAAGATGCAGCGCAAAgg                                                                                                          >  1:261058/1‑150 (MQ=255)
                     ttGCATGAGCGGCGGCTGGACCGCGAGCGCTTCATCAGCGACGTGATGAACCAGCTGCAGAACGAGTTGCTCGCCACTGGCGTGAAGGCCGACATCAGCGGCCGGGCGAAACATGTCTATTCGATCTGGCGCAAGATGCAGCGCAAAGGc                                                                                                         <  1:516443/150‑1 (MQ=255)
                     ttGCATGAGCGGCGGCTGGACCGCGAGCGCTTCATCAGCGACGTGATGAACCAGCTGCAGAACGAGTTGCTCGCCACTGGCGTGAAGGCCGACATCAGCGGCCGGGCGAAACATGTCTATTCGATCTGGCGCAAGATGCAGCGCAAAGGc                                                                                                         <  2:524129/150‑1 (MQ=255)
                          tGAGCGGCGGCTGGACCGCGAGCGCTTCATCAGCGACGTGATGAACCAGCTGCAGAACGAGTTGCTCGCCACTGGCGTGAAGGCCGACATCAGCGGCCGGGCGAAACATGTCTATTCGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGa                                                                                                    >  1:551113/1‑150 (MQ=255)
                          tGAGCGGCGGCTGGACCGCGAGCGCTTCATCAGCGACGTGATGAACCAGCTGCAGAACGAGTTGCTCGCCACTGGCGTGAAGGCCGACATCAGCGGCCGGGCGAAACATGTCTATTCGACCTGGCGCAAGACGCCGCCCAAAGGCCTGGa                                                                                                    <  2:1090807/150‑1 (MQ=255)
                               ggcggcTGGACCGCGAGCGCTTCATCAGCGACGTGATGAACCAGCTGCAGAACGAGTTGCTCGCCACTGGCGTGAAGGCCGACATCAGCGGCCGGGCGAAACATGTCTATTCGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCa                                                                                               <  2:901251/150‑1 (MQ=255)
                                 cggcTGGACCGCGAGCGCTTCATCAGCGACGTGATGAACCAGCTGCAGAACGAGTTGCTCGCCACTGGCGTGAAGGCCGACATCAGCGGCCGGGCGCGCCGCAATTATTCGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGc                                                                                             >  1:83053/1‑150 (MQ=255)
                                     tGGACCGCGAGCGCTTCATCAGCGACGTGATGAACCAGCTGCAGAACGAGTTGCTCGCCACTGGCGTGAAGGACGACATCAGCGGCCGGGCGAAACATGTCTATTCGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGa                                                                                         >  2:110440/1‑150 (MQ=255)
                                        aCCGCGGGCGCGTCAGCAGCGACGTGATGAATTAGCTGCAGACCGAGTTGCTCGCCACTGGCGTGAAGGCCGACATCAGCGGCCGGGCGAAACATGTCTATTCGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCt                                                                                      <  2:564530/150‑1 (MQ=255)
                                                cgcTTCATCAGCGACGTGATGAACCAGCTGCAGAACGAGTTGCTCGCCACTGGCGTGAAGGCCGACATCAGCGGCCGGGCGAAACATGTCTATTCGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTACGAcgtg                                                                              <  2:1122124/150‑1 (MQ=255)
                                                   ttCATCAGCGACGTGATGAACCAGCTGCAGAACGAGTTGCTCGCCACTGGCGTGAAGGCCGACATCAGCGGCCGGGCGAAACATGTCTATTCGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTACGAcgtgcgt                                                                           >  1:75257/1‑150 (MQ=255)
                                                        caGCGACGTGATGAACCAGCTGCAGAACGAGTTGCTCGCCACTGGCGTGAAGGCCGACATCAGCGGCCGGGCGAAACATGTCTATTCGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTACGACGTGCgtgcggt                                                                      >  2:726445/1‑150 (MQ=255)
                                                         aGCGACGTGATGAACCAGCTGCAGAACGAGTTGCTCGCCACTGGCGTGAAGGCCGACATCAGCGGCCGGGCGAAACATGTCTATTCGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTACGACGTGCgtgcggtg                                                                     >  1:334922/1‑150 (MQ=255)
                                                          gcaaCGTGATGAACCAGCTGCAGAACGAGTTGCTCGCAACTGGCGTGAAGGCCGACATAAGCGGCCGGGCGAAACATGTCTATTCGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGAGCTACGACGTGCgtgcggtgc                                                                    >  2:446798/4‑150 (MQ=255)
                                                               gTGATGAACCAGCTGCAGAACGAGTTGCTCGCCACTGGCGTGAAGGCCGACATCAGCGGCCGGGCGAAACATGTCTATTCGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTACGACGTGCGTGCGGTGCGCGTg                                                               <  1:782236/150‑1 (MQ=255)
                                                                  atgaACCAGCTGCAGAACGAGTTGCTCGCCACTGGCGTGAAGGCCGACATCAGCGGCCGGGCGAAACATGTCTATTCGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTACGACGTGCGTGCGGTGCGCGTGCTg                                                            <  2:607630/150‑1 (MQ=255)
                                                                       ccAGCTGCAGAACGAGTTGCTCGCCACTGGCGTGAAGGCCGACATCAGCGGCCGGGCGAAACATGTCTATTCGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTACGACGTGCGTGCGGTGCGCGTGCTGGTGcc                                                       >  1:656317/1‑150 (MQ=255)
                                                                       ccAGCTGCAGAACGAGTTGCTCGCCACTGGCGTGAAGGCCGACATCAGCGGCCGGGCGAAACATGTCTATTCGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTACGACGTGCGTGCGGTGCGCGTGCTGGTGcc                                                       <  2:928632/150‑1 (MQ=255)
                                                                                gAACGAGTTGCTCGCCACTGGCGTGAAGGCCGACATCAGCGGCCGGGCGAAACATGTCTATTCGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTACGACGTGCGTGCGGGGCGCGTGCTGGCGCCGGAAATCcg                                              >  1:1094461/1‑150 (MQ=255)
                                                                                     aGTTGCTCGCCACTGGCGTGAAGGCCGACATCAGCGGCCGGGCGAAACATGTCTATTCGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTACGACGTGCGTGCGGTGCGCGTGCTGGTGCCGGAAATCCGCGACt                                         <  1:582497/150‑1 (MQ=255)
                                                                                                    gCGTGAAGGCCGACATCAGCGGCCGGGCGAAACATGTCTATTCGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTACGACGTGCGTGCGGTGCGCGTGCTGGTGCCGGAAATCCGCGACTGCTACACCGCGCTgg                          >  2:986225/1‑150 (MQ=255)
                                                                                                    gCGTGAAGGCCGACATCAGCGGCCGGGCGAAACATGTCTATTCGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTACGACGTGCGTGCGGTGCGCGTGCTGGTGCCGGAAATCCGCGACTGCTACACCGCGCTgg                          >  1:935971/1‑150 (MQ=255)
                                                                                                     cttGAAGGCGGATATCAGCGGCCGGGCGAAACATGTCTATTCGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTACGACGTGCGTGCGGTGCGCGTGCTGGTGCCGGAAATCCGCGACTGCTACACCGCGCTggg                         >  2:730225/3‑150 (MQ=255)
                                                                                                      gTGAAGGCCGACATCAGCGGCCGGGCGAAACATGTCTATTCGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTACGACGTGCGTGCGGTGCGCGTGCTGGTGCCGGAAATCCGCGACTGCTACACCGCGCTGGGc                        >  2:769564/1‑150 (MQ=255)
                                                                                                         aaGGCCGACATCAGCGGCCGGGCGAAACATGTCTATTCGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTACGACGTGCGTGCGGTGCGCGTGCTGGTGCCGGAAATCCGCGACTGCTACACCGCGCTGGGCATc                     >  1:1117306/1‑150 (MQ=255)
                                                                                                           ggCCGACATCAGCGGCCGGGCGAAACATGTCTATTCGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTACGACGTGCGTGCGGTGCGCGTGCTGGTGCCGGAAATCCGCGACTGCTACACCGCGCTGGGCATCGt                   <  1:769564/150‑1 (MQ=255)
                                                                                                                 cATCAGCGGCCGGGCGAAACATGTCTATTCGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTACGACGTGCGTGCGGTGCGCGTGCTGGTGCCGGAAATCCGCGACTGCTACACCGCGCTGGGCATCGTGCacac             <  2:772320/150‑1 (MQ=255)
                                                                                                                       cGGCCGGGCGAAACATGTCTATTCGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTACGACGTGCGTGCGGTGCGCGTGCTGGTGCCGGAAATCCGCGACTGCTACACCGCGCTGGGCATCGTGCACACCTtgtg       <  2:77557/150‑1 (MQ=255)
                                                                                                                           cGGGCGAAACATGTCTATTCGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTACGACGTGCGTGCGGTGCGCGTGCTGGTGCCGGAAATCCGCGACTGCTACACCGCGCTGGGCATCGTGCACACCTTGTggcgg   >  1:743385/1‑150 (MQ=255)
                                                                                                                            gggCGAAACATGTCTATTCGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTACGACGTGCGTGCGGTGCGCGTGCTGGTGCCGGAAATCCGCGACTGCTACACCGCGCTGGGCATCGTGCACACCTTGTggcggc  <  2:1071489/150‑1 (MQ=255)
                                                                                                                                       |                                                                                                                                          
TACAAGCAGATCGCCAAGCTGTTGCATGAGCGGCGGCTGGACCGCGAGCGCTTCATCAGCGACGTGATGAACCAGCTGCAGAACGAGTTGCTCGCCACTGGCGTGAAGGCCGACATCAGCGGCCGGGCGAAACATATCTATTCGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTACGACGTGCGTGCGGTGCGCGTGCTGGTGCCGGAAATCCGCGACTGCTACACCGCGCTGGGCATCGTGCACACCTTGTGGCGGC  >  NC_002947/1851620‑1851893

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑