Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC	NC_002947	4,964,829	(CGCGGCGCTT)1→2	coding (574/1476 nt)	fleQ ←	transcriptional regulator FleQ

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_002947	4964812 =	7 (0.140)	49 (1.040)	41/252	0.2	87.8%	coding (591/1476 nt)	fleQ	transcriptional regulator FleQ
	?	NC_002947	= 4964821	7 (0.150)					coding (582/1476 nt)	fleQ	transcriptional regulator FleQ

TGGTTGGCACCAGTCGTGCCATTCAGCACGTGCGGCAGATGATGCAGCAAGTGGCGGATACCGACGCCAGCGTGCTGATCCTGGGTGAGTCCGGCACCGGCAAGGAAGTGGTAGCGCGCAACCTGCACTACCATTCCAAGCGCCGCGAAGCGCCG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NC_002947/4964966‑4964812 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑aagcgccgCGAAGCGCCGTTCGTGCCGGTCAACTGTGGCGCGATCCCGGCCGAGCTGCTGGAAAGCGAACTGTTCGGCCACGAGAAGGGCGCCTTTACCGGGGCCATCACCAGCCGTGCCGGGCGTTTCGAACTGGCCAACGGCGGTACCCTGT  <  NC_002947/4964821‑4964676                                                                                                                                                                                                                                                                                                               TGGTTGGCACCAGTCGTGCCATTCAGCACGTGCGGCAGATGATGCAGCAAGTGGCGGATACCGACGCCAGCGTGCTGATCCTGGGTGAGTCCGGCACCGGCAAGGAAGTGGTAGCGCGCAACCTGCACTACCATTCCAAGCGCCGCGAAG                                                                                                                                                         >  2:654198/1‑150         ACCAGTCGTGCCATTCAGCACGTGCGGCAGATGATGCAGCAAGTGGCGGATACCGACGCCAGCGTGCTGATCCTGGGTGAGTCCGGCACCGGCAAGGAAGTGGTAGCGCGCAACCTGCACTACCATTCCAAGCGCCGCGAAGCGCCGCGA                                                                                                                                                 >  2:139503/1‑150                 TGCCATTCAGCACGTACGGCAGATGATGCAGCAAGTGGCGGATACCGACGCCAGCGTGCTGATCCTGGGTGAGTCCGGCACCGGCAAGGAAGTGGTAGCGCGCAACCTGCACTACCATTCCAAGCGCCGCGAAGCGCCGCGAAGCGCCGT                                                                                                                                         <  2:878973/150‑1                    CATTCAGCACGTGCGGCAGATGATGCAGCAAGTGGCGGATACCGACGCCAGCGTGCTGATCCTGGGTGAGTCCGGCACCGGCAAGGAAGTGGTAGCGCGCAACCTGCACTACCATTCCAAGCGCCGCGAAGCGCCGCGAAGCGCCGTTCG                                                                                                                                      >  2:477325/1‑150                     ATTCAGCACGTGCGGCAGATGATGCAGCAAGTGGCGGATACCGACGCCAGCGTGCTGATCCTGGGTGAGTCCGGCACCGGCAAGGAAGTGGTAGCGCGCAACCTGCACTACCATTCCAAGCGCCGCGAAGCGCCGCGAAGCGCCGTTCGT                                                                                                                                     >  2:87180/1‑150                      TTCAGCACGTGCGGCAGATGATGCAGCAAGTGGCGGATACCGACGCCAGCGTGCTGATCCTGGGTGAGTCCGGCACCGGCAAGGAAGTGGTAGCGCGCAACCTGCACTACCATTCCAAGCGCCGCGAAGCGCCGCGAAGCGCCGTTCGTG                                                                                                                                    <  2:1162600/150‑1                          GCACGTGCGGCAGATGATGCAGCAAGTGGCGGATACCGACGCCAGCGTGCTGATCCTGGGTGAGTCCGGCACCGGCAAGGAAGTGGTAGCGCGCAACCTGCACTACCATTCCAAGCGCCGCGAAGCGCCGCGAAGCGCCGTTCGTGCCGG                                                                                                                                >  2:704639/1‑150                                    CAGATGATGCAGCAAGTGGCGGATACCGACGCCAGCGTGCTGATCCTGGGTGAGTCCGGCACCGGCAAGGAAGTGGTAGCGCGCAACCTGCACTACCATTCCAAGCGCCGCGAAGCGCCGCGAAGCGCCGTTCGTGCCGGTCAACTGTGG                                                                                                                      >  2:1258335/1‑150                                     AGATGATGCAGCAAGTGGCGGACACCTACGGCACGGTGCTGATGCTGGGTGAGTCCGGCACCGACAAGGAAGTGGTAGCGCGCAACCTGCACTACCATTCCAAGAGCCGCGAAGCGCCGCGAACCGCCGTTCGTGCCGGACAACCGTGGC                                                                                                                     >  2:750097/1‑150                                         GATGCAGCAAGTGGCGGATACCGACGCCAGCGTGCTGATCCTGGGTGAGTCCGGCACCGGCAAGGAAGTGGTAGCGCGCAACCTGCACTACCATTCCAAGCGCCGCGAAGCGCCGCGAAGCGCCGTGCGTGCCGGTCAACTGTGGCGCGA                                                                                                                 <  1:139503/150‑1                                               GCAAGTGGCGGATACCGACGCCAGCGTGCTGATCCTGGGTGAGTCCGGCACCGGCAAGGAAGTGGTAGCGCGCAACCTGCACTACCATTCCAAGCGCCGCGAAGCGCCGCGAAGCGCCGTTCGTGCCGGTCAACTGTGGCGCGATCCCGG                                                                                                           >  1:477089/1‑150                                                   GTGGCGGATACCGACGCCAGCGTGCTGATCCTGGGTGAGTCCGGCACCGGCAAGGAAGTGGTAGCGCGCAACCTGCACTACCATTCCAAGCGCCGCGAAGCGCCGCGAAGCGCCGTTCGTGCCGGTCAACTGTGGCGCGATCCCGGCCGA                                                                                                       >  1:11049/1‑150                                                          ATACCGACGCCAGCGTGCTGATCCTGGGTGAGTCCGGCACCGGCAAGGAAGTGGTAGCGCGCAACCTGCACTACCATTCCAAGCGCCGCGAAGCGCCGTGAAGCGCCGTTCGTGCCGGTCAACTGTGGCGCGATCCCGGCCGAGCTGCTG                                                                                                <  2:536787/150‑1                                                           TACCGACGCCAGCGTGCTGATCCTGGGTGAGTCCGGCACCGGCAAGGAAGTGGTAGCGCGCAACCTGCACTACCATTCCAAGCGCCGCGAAGCGCCGCGAAGCGCCGTTCGTGCCGGTCAACTGTGGCGCGATCCCGGCCGAGCTGCTGG                                                                                               <  1:87180/150‑1                                                              CGACGCCAGCGTGCTGATCCTGGGTGAGTCCGGCACCGGCAAGGAAGTGGTAGCGCGCAACCTGCACTACCATTCCAAGCGCCGCGAAGCGCCGCGAAGCGCCGTTCGTGCCGGTCAACTGTGGCGCGATCCCGGCCGAGCTGCTGGAAA                                                                                            >  2:961785/1‑150                                                              CGACGCCAGCGTGCTGATCCTGGGTGAGTCCGGCACCGGCAAGGAAGTGGTAGCGCGCAACCTGCACTACCATTCCAAGCGCCGCGAAGCGCCGCGAAGCGCCGTTCGTGCCGGTCAACTGTGGCGCGATCCCGGCCGAGCTGCTGGAAA                                                                                            >  2:825944/1‑150                                                               GACGCCAGCGTGCTGATCCTGGGTGAGTCCGGCACCGGCAAGGAAGTGGTAGCGCGCAACCTGCACTACCATTCCAAGCGCCGCGAAGCGCCGCGAAGCGCCGTTCGTGCCGGTCAACTGTGGCGCGATCCCGGCCGAGCTGCTGGAAA                                                                                            <  1:311820/149‑1                                                               GACGCCAGCGTGCTGATCCTGGGTGAGTCCGGCACCGGCAAGGAAGTGGTAGCGCGCAACCTGCACTACCATTCCAAGCGCCGCGAAGCGCCGCGAAGCGCCGTTCGTGCCGGTCAACTGTGGCGCGATCCCGGCCGAGCTGCTGGAAA                                                                                            >  2:311820/1‑149                                                                       gaTGCTCATCCTGGGTGAGTCCGGCACGGGCAAGGAAGTGGTAGCGCGCAACCTGCACTACCATTCCAAGCGCCGCGAAGCGCCGCGAAGCGGCGTGCGGCCTGGTCAACGGTGGCGCTATTCCGGCCGAGTTGCTGGAAAGCGAACTGT                                                                                   <  2:492768/148‑1                                                                          GCTGATCCTGGGTGAGTCCGGCACCGGCAAGGAAGTGGTAGCGCGCAACCTGCACTACCATTCCAAGCGCCGCGAAGCGCCGCGAAGCGCCGTTCGTGCCGGTCAACTGTGGCGCGATCCCGGCCGAGCTGCTGGAAAGCGAACTGTTCG                                                                                <  1:346528/150‑1                                                                          GCTGATCCTGGGTGAGTCCGGCACCGGCAAGGAAGTGGTAGCGCGCAACCTGCACTACCATTCCAAGCGCCGCGAAGCGCCGCGAAGCGCCGTTCGTGCCGGTCAACTGTGGCGCGATCCCGGCCGAGCTGCTGGAAAGCGAACTGTTCG                                                                                <  2:451973/150‑1                                                                             GATCCTGGGTGAGTCCGGCACCGGCAAGGAAGTGGTAGCGCGCAACCTGCACTACCATTCCAAGCGCCGCGAAGCGCCGCGAAGCGCCGTTCGTGCCGGTCAACTGTGGCGCGATCCCGGCCGA                                                                                                       <  1:1190456/124‑1                                                                             GATCCTGGGTGAGTCCGGCACCGGCAAGGAAGTGGTAGCGCGCAACCTGCACTACCATTCCAAGCGCCGCGAAGCGCCGCGAAGCGCCGTTCGTGCCGGTCAACTGTGGCGCGATCCCGGCCGA                                                                                                       >  2:1190456/1‑124                                                                              ATCCTGGGTGAGTCCGGCACCGGCAAGGAAGTGGTAGCGCGCAACCTGCACTACCATTCCAAGCGCCGCGAAGCGCCGCGAAGCGCCGTTCGTGCCGGTCAACTGTGGCGCGATCCCGGCCGAGCTGCTGGAAAGCGAACTGTTCGGCCA                                                                            >  1:1011430/1‑150                                                                                CCTGGGTGAGTCCGGCACCGGCAAGGAAGTGGTAGCGCGCAACCTGCACTACCATTCCAAGCGCCGCGAAGCGCCGCGAAGCGCCGTTCGTGCCGGTCAACTGTGGCGCGATCCCGGCCGAGCTGCTGGAAAGCGAA                                                                                       <  1:169083/137‑1                                                                                CCTGGGTGAGTCCGGCACCGGCAAGGAAGTGGTAGCGCGCAACCTGCACTACCATTCCAAGCGCCGCGAAGCGCCGCGAAGCGCCGTTCGTGCCGGTCAACTGTGGCGCGATCCCGGCCGAGCTGCTGGAAAGCGAA                                                                                       >  2:169083/1‑137                                                                                 CTGGGTGAGTCCGGCACCGGCAAGGAAGTGGTAGCGCGCAACCTGCACTACCATTCCAAGCGCCGCGAAGCGCCGCGAAGCGCCGTTCGTGCCGGTCAACTGTGGCGCGATCCCGGCCGAGCTGCTGGAAAGCGAACTGTTCGGCCACGA                                                                         >  2:1256431/1‑150                                                                                       GAGTCCGGCACCGGCAAGGAAGTGGTAGCGCGCAACCTGCACTACCATTCCAAGCGCCGCGAAGCGCCGCGAAGCGCCGTTCGTGCCGGTCAACTGTGGCGCGATCCCGGCCGAGCTGCTGGAAAGCGAACTGTTCGGCCACGAGAAGGG                                                                   >  2:488442/1‑150                                                                                         GTCCGGCACCGGCAAGGAAGTGGTAGCGCGCAACCTGCACTACCATTCCAAGCGCCGCGAAGCGCCGCGAAGCGCCGTTCGTGCCGGTCAACTGTGGCGCGATCCCGGCCGAGCTGCTGGAAAGCGAACTGTTCGGCCACGAGAAGGGCG                                                                 >  2:1189158/1‑150                                                                                         GTCCGGCACCGGCAAGGAAGTGGTAGCGCGCAACCTGCACTACCATTCCAAGCGCCGCGAAGCGCCGCGAAGCGCCGTTCGTGCCGGTCAACTGTGGCGCGATCCCGGCCGAGCTGCTGGAAAGCGAACTGTTCGGCCACGAGAAGGGCG                                                                 >  2:555823/1‑150                                                                                          TCCGGCACCGGCAAGGAAGTGGTAGCGCGCAACCTGCACTACCATTCCAAGCGCCGCGAAGCGCCGCGAAGCGCCGTTCGTGCCGGTCAACTGTGGCGCGATCCCGGCCGAGCTGCTGGAAAGCGAACTGTTCGGCCACGAGAAGGGCGC                                                                >  2:836798/1‑150                                                                                          TCCGGCACCGGCAAGGAAGTGGTAGCGCGCAACCTGCACTACCATTCCAAGCGCCGCGAAGCGCCGCGAAGCGCCGTTCGTGCCGGTCAACTGTGGCGCGATCCCGGCCGAGCTGCTGGAAAGCGAACTGTTCGGCCACGAGAAGGGCGC                                                                >  1:544211/1‑150                                                                                          TCCGGCACCGGCAAGGAAGTGGTAGCGCGCAACCTGCACTACCATTCCAAGCGCCGCGAAGCGCCGCGAAGCGCCGTTCGTGCCGGTCAACTGTGGCGCGATCCCGGCCGAGCTGCTGGAAAGCGAACTGTTCGGCCACGAGAAGGGCGC                                                                >  2:738876/1‑150                                                                                               CACCGGCAAGGAAGTGGTAGCGCGCAACCTGCACTACCATTCCAAGCGCCGCGAAGCGCCGCGAAGCGCCGTTCGTGCCGGTCAACTGTGGCGCGATCCCGGCCGAGCTGCTGGAAAGCGAACTGTTCGGCCACGAGAAGGGCGCCTTTA                                                           <  2:69609/150‑1                                                                                                  CGGCAAGGAAGTGGTAGCGCGCAACCTGCACTACCATTCCAAGCGCCGCGAAGCGCCGCGAAGCGCCGTTCGTGCCGGTCAACTGTGG                                                                                                                      >  2:523152/1‑88                                                                                                  CGGCAAGGAAGTGGTAGCGCGCAACCTGCACTACCATTCCAAGCGCCGCGAAGCGCCGCGAAGCGCCGTTCGTGCCGGTCAACTGTGG                                                                                                                      <  1:523152/88‑1                                                                                                   GGCAAGGAAGTGGTAGCGCGCAACCTGCACTACCATTCCAAGCGCCGCGAAGCGCCGCGAAGCGCCGTTCGCGCCGGTCAACTGTGGCGCGATCCCGGCCGAGCTGCTGGAAAGCGAACTGTTCGGCCACGAGAAGGGCGCCTCTACCGG                                                       <  1:477325/150‑1                                                                                                    GCAAGGAAGTGGTAGCGCGCAACCTGCACTACCATTCCAAGCGCCGCGAAGCGCCGCGAAGCGCCGTTCGTGCCGGTCAACTGTGGCGCGATCCCGGCCGAGCTGCTGGAAAGCGAACTGTTCGGCCACGAGAAGGGCGCCTTTACCGGG                                                      <  1:704639/150‑1                                                                                                    GCAAGGAAGTGGTAGCGCGCAACCTGCACTACCATTCCAAGCGCCGCGAAGCGCCGCGAAGCGCCGTTCGTGCCGGTCAACTGTGGCGCGATCCCGGCCGAGCTGCTGGAAAGCGAACTGTTCGGCCACGAGAAGGGCGCCTTTACCGGG                                                      >  2:1123264/1‑150                                                                                                    GCAAGGAAGTGGTAGCGCGCAACCTAATCAACCATTCCAAGCGCCGCGAAGCGCCGCGAAGCGCCGTTCGTGCCGGTCAACTGTGGCGCGATCCCGGCCGAGCTGCTGGAAAGCGAACTGTTCGGCCACGAGAAGGGCGCCTTTACCGGG                                                      >  1:664888/1‑150                                                                                                            GTGGTAGCGCGCAACCTGCACTACCATTCCAAGCGCCGCGAAGCGCCGCGAAGCGCCGTTCGTGCCGGTCAACTGTGGCGCGATCCCGGCCGAGCTGCTGGAAAGCGAACTGTTCGGCCACGAGAAGGGCGCCTTTACCGGGGCCATCAC                                              <  1:836798/150‑1                                                                                                              GGTAGCGCGCAACCTGCACTACCATTCCAAGCGCCGCGAAGCGCCGCGAAGCGCCGTTCGTGCCGGTCAACTGTGGCGCGATCCCGGCCGAGCTGCTGGAAAGCGAACTGTTCGGCCACGAGAAGGGCGCCTTTACCGGGG                                                     <  1:743843/141‑1                                                                                                              GGTAGCGCGCAACCTGCACTACCATTCCAAGCGCCGCGAAGCGCCGCGAAGCGCCGTTCGTGCCGGTCAACTGTGGCGCGATCCCGGCCGAGCTGCTGGAAAGCGAACTGTTCGGCCACGAGAAGGGCGCCTTTACCGGGG                                                     >  2:743843/1‑141                                                                                                                          CCTGCACTACCATTCCAAGCGCCGCGAAGCGCCGCGAAGCGCCGTTCGTGCCGGTCAACTGTGGCGCGATCCCGGCCGAGCTGCGGGAAAGTGAACTGTTCGGCCACGAGAAGGGCGCCTTTACCGGGGCCATCACCAGCCGTGCCGGGC                                <  1:750097/150‑1                                                                                                                           cgGCACTACCATTCCAAGCGCCGCGAAGCGCCGCGAAGCGCCGTTCGTGCCGGTCAACTGTGGCGCGATCCCGGCCGAGCTGCTGGAAAGCGAACTGTTCG                                                                                <  1:913766/99‑1                                                                                                                           CTGCACTACCATTCCAAGCGCCGCGAAGCGCCGCGAAGCGCCGTTCGTGCCGGTCAACTGTGGCGCGATCCCGGCCGAGCTGCTGGAAAGCGAACTGTTCG                                                                                >  2:913766/1‑101                                                                                                                                CTACCATTCCAAGCGCCGCGAAGCGCCGCGAAGCGCCGTTCGTGCCGGTCAACTGTGGCGCGATCCCGGCCGAGCTGCTGGAAAGCGAACTGTTCGGCCACGAGAAGGGCGCCTTTACCGGAGCCATCACCAGCCGTGCCGGGCGTTTCG                          >  1:875202/1‑150                                                                                                                                  ACCATTCCAAGCGCCGCGAAGCGCCGCGAAGCGCCGTTCGTGCCGGTCAACTGTGGCGCGATCCCGGCCGAGCTGCTGGAAAGCGAACTGTTCGGCCACGAGAAGGGCGCCTTTACCGGGGCCATCACCAGCCGTGCCGGGCGTTTCGAA                        <  2:1011430/150‑1                                                                                                                                          AAGCGCCGCGAAGCGCCGCGAAGCGCCGTTCGTGCCGGTCAACTGTGGCGCGATCCCGGCCGAGCTGCTGGAAA                                                                                            <  1:951217/74‑1                                                                                                                                          AAGCGCCGCGAAGCGCCGCGAAGCGCCGTTCGTGCCGGTCAACTGTGGCGCGATCCCGGCCGAGCTGCTGGAAA                                                                                            >  2:951217/1‑74                                                                                                                                                        GCCGCGAAGCGCCGTTCGTGCCGGTCAACTGTGGCGCGATCCCGGCCGAGCTGCTGGAAAGCGAACTGTTCGGCCACGAGAAGGGCGCCTTTACCGGGGCCATCACCAGCCGTGCCGGGCGTTTCGAACTGGCCAACGGCGGTACCCTGT  <  2:11049/150‑1                                                                                                                                                                                                                                                                                                               TGGTTGGCACCAGTCGTGCCATTCAGCACGTGCGGCAGATGATGCAGCAAGTGGCGGATACCGACGCCAGCGTGCTGATCCTGGGTGAGTCCGGCACCGGCAAGGAAGTGGTAGCGCGCAACCTGCACTACCATTCCAAGCGCCGCGAAGCGCCG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NC_002947/4964966‑4964812 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑aagcgccgCGAAGCGCCGTTCGTGCCGGTCAACTGTGGCGCGATCCCGGCCGAGCTGCTGGAAAGCGAACTGTTCGGCCACGAGAAGGGCGCCTTTACCGGGGCCATCACCAGCCGTGCCGGGCGTTTCGAACTGGCCAACGGCGGTACCCTGT  <  NC_002947/4964821‑4964676

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.