Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
JC	NC_002947	1,851,813	+CGTG	88.8%	coding (857/2241 nt)	relA →	ATP:GTP 3'‑pyrophosphotransferase

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_002947	= 1851813	1 (0.070)	8 (0.510) +CGTG	7/228	NT	88.8%	coding (857/2241 nt)	relA	ATP:GTP 3'‑pyrophosphotransferase
	?	NC_002947	1851814 =	1 (0.060)					coding (858/2241 nt)	relA	ATP:GTP 3'‑pyrophosphotransferase

AACGAGTTGCTCGCCACTGGCGTGAAGGCCGACATCAGCGGCCGGGCGAAACATATCTATTCGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTACGA‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_002947/1851701‑1851813 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑CGTGCGTGCGGTGCGCGTGCTGGTGCCGGAAATCCGCGACTGCTACACCGCGCTGGGCATCGTGCACACCTTGTGG  >  NC_002947/1851814‑1851889                                                                                                                  ||||                                                                             AACGAGTTGCTCGCCACTGGCGTGAAGGCCGACATCAGCGGCCGGGCGAAACATATCTATTCGATCTGGTGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTACGACGTGCGTGCGTGCGGTGCGCGTGCTGGTGCCGGAAAT                                             >  1:81113/1‑150   CGAGTTGCTCGCCACTGGCGTGAAGGCCGACATCAGCGGCCGGGCGAAACATATCTATTCGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTACGACGTGCGTGCGTGCGGTGCGCGTGCTGGTGCCGGAAATCC                                           <  1:60958/150‑1                ACTGGCGTGAAGGCCGACATCAGCGGCCGGGCGAAACATATCTATTCGATCTGGTGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTACGACGTGCGTGCGTGCGGTGCGCGTGCTGGTGCCGGAAATCCGCGACTGCTACAC                              <  2:81113/150‑1                                            GGGCGAAACATATCTATTCGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTACGACGTGCGTGCGTGCGGTGCGCGTGCTGGTGCCGGAAATCCGCGACTGCTACACCGCGCTGGGCATCGTGCACACCTTGTGG  <  2:441121/150‑1                                                     ATATCTATTCGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTACGACGTGCGTGCGTGCGGTGCGCGTGCTGGTGCCGGAAATCCGCGACTGCTACACCGCGCTGG                      >  1:110986/1‑121                                                     ATATCTATTCGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTACGACGTGCGTGCGTGCGGTGCGCGTGCTGGTGCCGGAAATCCGCGACTGCTACACCGCGCTGG                      <  2:110986/121‑1                                                           ATTCGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTACGACGTGCGTGCGTGCGGTGCGCGTGCTGGTGCCGGAAATCCGCGACTGCTACACCGCGCTGG                      <  1:94717/115‑1                                                           ATTCGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTACGACGTGCGTGCGTGCGGTGCGCGTGCTGGTGCCGGAAATCCGCGACTGCTACACCGCGCTGG                      >  2:94717/1‑115                                                                                                                  ||||                                                                             AACGAGTTGCTCGCCACTGGCGTGAAGGCCGACATCAGCGGCCGGGCGAAACATATCTATTCGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTACGA‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_002947/1851701‑1851813 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑CGTGCGTGCGGTGCGCGTGCTGGTGCCGGAAATCCGCGACTGCTACACCGCGCTGGGCATCGTGCACACCTTGTGG  >  NC_002947/1851814‑1851889

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.