Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 89,439 T→C 11.7% L5P (CTT→CCT)  PP_0079 → transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_00294789,4390TC11.7% 84.9 / 3.2 34L5P (CTT→CCT) PP_0079transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base T (15/15);  new base C (2/2);  total (17/17)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.32e-01

TATTCGGACGCGTCATGGTGGCTTCCATCGTTGAGGGTTATGCGAACCGCTTGCCTACCAAGATGCGACTGTGGATAACATGGGTAAAGCCCATGGCGGGCAATGACTGGGATTAGCCAAGCTTATGTTTGAGCACCTTGCCGGGTTGTCGCTGGATACCTTGCGCGTATTCGAGGCTGCTGCGCGCCTGCGTGGCTTTACCGCTGCGGCGCTGGAGTTGGGTACCACGCAGCCGGCAGTGAGCCAGCAGGTGAAGCGCCTGGAGGCACAGTTGGGCACGCGCCTGT  >  NC_002947/89302‑89588
                                                                                                                                         |                                                                                                                                                     
tatTCGGACGCGTCATGGTGGCTTCCATCGTTGAGGGTTATGCGAACCGCTTGCCTACCAAGATGCGACTGTGGATAACATGGGTAAAGCCCATGGCGGGCAATGACTGGGATTAGCCAAGCTTATGTTTGAGCACCTTGCCGGGTTGtt                                                                                                                                           >  2:388935/1‑149 (MQ=255)
  ttCGGACGCGTCATGGTGGCTTCCATCGTTGAGGGTTATGCGAACCGCTTGCCTACCAAGATGCGACTGTGGATAACATGGGTAAAGCCCATGGCGGGCAATGACTGGGATTAGCCAAGCTTATGTTTGAGCACCTTGCCgg                                                                                                                                                 >  2:272925/1‑142 (MQ=255)
  ttCGGACGCGTCATGGTGGCTTCCATCGTTGAGGGTTATGCGAACCGCTTGCCTACCAAGATGCGACTGTGGATAACATGGGTAAAGCCCATGGCGGGCAATGACTGGGATTAGCCAAGCTTATGTTTGAGCACCTTGCCgg                                                                                                                                                 <  1:272925/142‑1 (MQ=255)
       aCGTGTCATGGTGGCTTCCATCGTTGAGGGTTATGCGAACCGCTTGCCTACCAAGATGCGACTGTGGATAACATGGGTAAAGCCCATGGCGGGCAATGACTGGGATTAGCCAAGCTTATGTTTGAGCACCCTGCCGGGTTGTCGCTGGAt                                                                                                                                    >  2:198619/1‑150 (MQ=255)
                ggtggCTTCCATCGTTGAGGGTTATGCGAACCGCTTGCCTACCAAGATGAGACTGTGGATAACATGGGTAAAGCCCATGGCGGGCAATGACTGGGATTAGCCAAGCTTATGTTTGAGCACCTTGCCGGGTTGTCGCTGGATACCTTgcgc                                                                                                                           <  1:389384/150‑1 (MQ=255)
                      ttCCATCGTTGAGGGTTATGCGAACCGCTTGCCTACCAAGATGCGACTGTGGATAACATGGGTAAAGCCCATGGCGGGCAATGACTGGGATTAGCCAAGCTTATGTTTGAGCACCTTGCCGGGTTGTCGCTGGATACCTTGCGCGTATTc                                                                                                                     >  2:131128/1‑150 (MQ=255)
                         cATCGTTGAGGGTTATGCGAACCGCTTGCCTACCAAGATGCGACTGTGGATAACATGGGTAAAGCCCATGGCGGGCAATGACTGGGATTAGCCAAGCTTATGTTTGAGCACCTTGCCGGGTTGTCGCTGGATACCTTGCGCGTATTCGa                                                                                                                   <  2:326430/149‑1 (MQ=255)
                         cATCGTTGAGGGTTATGCGAACCGCTTGCCTACCAAGATGCGACTGTGGATAACATGGGTAAAGCCCATGGCGGGCAATGACTGGGATTAGCCAAGCTTATGTTTGAGCACCTTGCCGGGTTGTCGCTGGATACCTTGCGCGTATTCGa                                                                                                                   >  1:326430/1‑149 (MQ=255)
                                     ttATGCGAACCGCTTGCCTACCAAGATGCGACTGTGGATAACATGGGTAAAGCCCATGGCGGGCAATGACTGGGATTAGCCAAGCTTATGTTTGAGCACCTTGCCGGGTTGTCGCTGGATACCTTTCGCGTATGCGAGTCTGCTgcgcgc                                                                                                      >  2:429874/1‑150 (MQ=255)
                                        tGCGAACCGCTTGCCTACCAAGATGCGACTGTGGATAACATGGGTAAAGCCCATGGCGGGCAATGACTGGGATTAGCCAAGCTTATGTTTGAGCACCTTGCCGGGTTGTCGCTGGATACCTTGCGCGTATTCGAGGCTGCTGCGCGCCTg                                                                                                   >  1:151379/1‑150 (MQ=255)
                                        tGCGAACCGCTTGCCTACCAAGATGCGACTGTGGATAACATGGGTAAAGCCCATGGCGGGCAATGACTGGGATTAGCCAAGCTTATGTTTGAGCACCTTGCCGGGTTGTCGCTGGATACCTTGCGCGTATTCGAGGCTGCTGCGCGCCTg                                                                                                   >  1:348236/1‑150 (MQ=255)
                                        tGCGAACCGCTTGCCTACCAAGATGCGACTGTGGATAACATGGGTAAAGCCCATGGCGGGCAATGACTGGGATTAGCCAAGCTTATGTTTGAGCACCCTGCCGGGTTGTCGCTGGATACCTTGCGCGTATTCGAGGCTGCTGCGCGCCTg                                                                                                   >  2:334379/1‑150 (MQ=255)
                                             aCCGCTTGCCTACCAAGATGCGACTGTGGATAACATGGGTAAAGCCCATGGCGGGCAATGACTGGGATTAGCCAAGCTTATGTTTGAGCACCCTGCCGGGTTGTCGCTGGATACCTTGCGCGTATTCGAGGCTGCTGCGCGCCTGCGTgg                                                                                              <  1:198619/150‑1 (MQ=255)
                                                gCTTGCCTACCAAGATGCGACTGTGGATAACATGGGTAAAGCCCATGGCGGGCAATGACTGGGATTAGCCAAGCTTATGTTTGAGCACCTTGCCGGGTTGTCGCTGGATACCTTGCGCGTATTCGAGGCTGCTGCGCGCCTGCGTGGCtt                                                                                           >  1:378680/1‑150 (MQ=255)
                                                             gATGCGACTGTGGATAACATGGGTAAAGCCCATGGCGGGCAATGACTGGGATAAGCACCGATTATGTTTGAGCTTTTTGTGGGGTTGTCGCTGGATACCTTGCGCGTATTCGAGGCTGCTGCGCGCCTGCGTGGCTTTACCGCTgcggcg                                                                              >  1:388491/1‑150 (MQ=255)
                                                                      gtgGATAACATGGGTAAAGCCCATGGCGGGCAATGACTGGGATTAGCCAAGCTTATGTTTGAGCACCTTGCCGGGTTGTCGCTGGATACCTTGCGCGTATTCGAGGCTGCTGCGCGCCTGCGTGGCTTTACCGCTGCGGCGCTGGAGTTg                                                                     <  1:388935/150‑1 (MQ=255)
                                                                       tgGATAACATGGGTAAAGCCCATGGCGGGCAATGACTGGGATTAGCCAAGCTTATGTTTGAGCACCTTGCCGGGTTGTCGCTGGATACCTTGCGCGTATTCGAGGCTGCTGCGCGCCTGCGTGGCTTTACCGCTGCGGCGCTGGAGTTgg                                                                    <  1:33992/150‑1 (MQ=255)
                                                                       tgGATAACATGGGTAAAGCCCATGGCGGGCAATGACTGGGATTAGCCAAGCTTATGTTTGAGCACCTTGCCGGGTTGTCGCTGGATACCTTGCGCGTATTCGAGGCTGCTGCGCGCCTGCGTGGCTTTACCGCTGCGGCGCTGGAGTTgg                                                                    <  1:441085/150‑1 (MQ=255)
                                                                       tgGATAACATGGGTAAAGCCCATGGCGGGCAATGACTGGGATTAGCCAAGCTTATGTTTGAGCACCTTGCCGGGTTGTCGCTGGATACCTTGCGCGTATTCGAGGCTGCTGCGCGCCTGCGTGGCTTTACCGCTGCGGCGCTGGAGTTgg                                                                    <  1:91762/150‑1 (MQ=255)
                                                                          aTAACATGGGTAAAGCCCATGGCGGGCAATGACTGGGATTAGCCAAGCTTATGTTTGAGCACCTTGCCGGGTTGTCGCTGGATACCTTGCGCGTATTCGAGGCTGCTGCGCGCCTGCGTGGCTTTACCGCTGCGGCGCTGGAGTTGGGTa                                                                 >  1:188291/1‑150 (MQ=255)
                                                                               aTGGGTAAAGCCCATGGCGGGCAATGACTGGGATTAGCCAAGCTTATGTTTGAGCACCTTGCCGGGTTGTCGCTGGATACCTTGCGCGTATTCGAGGCTGCTGCGCGCCTGCGTg                                                                                               <  1:122007/115‑1 (MQ=255)
                                                                               aTGGGTAAAGCCCATGGCGGGCAATGACTGGGATTAGCCAAGCTTATGTTTGAGCACCTTGCCGGGTTGTCGCTGGATACCTTGCGCGTATTCGAGGCTGCTGCGCGCCTGCGTg                                                                                               >  2:122007/1‑115 (MQ=255)
                                                                                                cGGGCAATGACTGGGATTAGCCAAGCTTATGTTTGAGCACCTTGCCGGGTTGTCGCTGGATACCTTGCGCGTATTCGAGGCTGCTGCGCGCCTGCGTGGCTTTACCGCTGCGGCGCTGGAGTTGGGTACCACGCAGCCGGCAGTGAGCca                                           <  1:338384/150‑1 (MQ=255)
                                                                                                    cAATGACTGGGATTAGCCAAGCTTATGTTTGAGCACCTTGCCGGGTTGTCGCTGGATACCTTGCGCGTATTCGAGGCTGCTGCGCGCCTGCGTGGCTTTACCGCTGCGGCGCTGGAGTTGGGTACCACGCAGCCGGCAGTGAGCcagcag                                       <  2:348236/150‑1 (MQ=255)
                                                                                                         aCTGGGATTAGCCAAGCTTATGTTTGAGCACCTTGCCGGGTTGTCGCTGGATACCTTGCGCGTATTCGAGGCTGCTGCGCGCCTGCGTGGCTTTACCGCTGCGGCGCTGGAGTTGGGTACCACGCAGCCGGCAGTGAGCCAGCAGGTGaa                                  >  1:44695/1‑150 (MQ=255)
                                                                                                           tGGGATTAGCCAAGCTTATGTTTGAGCACCTTGCCGGGTTGTCGCTGGATACCTTGCGCGTATTCGAGGCTGCTGCGCGCCTGCGTGGCTTTACCGCTGCGGCGCTGGa                                                                         <  2:23963/109‑1 (MQ=255)
                                                                                                           tGGGATTAGCCAAGCTTATGTTTGAGCACCTTGCCGGGTTGTCGCTGGATACCTTGCGCGTATTCGAGGCTGCTGCGCGCCTGCGTGGCTTTACCGCTGCGGCGCTGGa                                                                         >  1:23963/1‑109 (MQ=255)
                                                                                                                   gCCAAGCTTATGTTTGAGCACCTTGCCGGGTTGTCGCTGGATACCTTGCGCGTATTCGAGGCTGCTGCGCGCCTGCGTGGCTTTACCGCTGCGGCGCTGGAGTTGGGTACCACGCAGCCGGCAGTGAGCCAGCAGGTGAAGCGCCTGGAg                        <  2:388491/150‑1 (MQ=255)
                                                                                                                    ccAAGCTTATGTTTGAGCACCCTGCCGGGTTGTCGCTGGATACCTTGCGCGTATTCGAGGCTGCTGCGCGCCTGCGTGGCTTTACCGCTGCGGCGCTGGAGTTGGGTACCACGCAGCCGGCAGTGAGCCAGCAGGTGAAGCGCCTGGAgg                       <  1:334379/150‑1 (MQ=255)
                                                                                                                          ttATGTTTGAGCACCTTGCCGGGTTGTCGCTGGATACCTTGCGCGTATTCGAGGCTGCTGCGCGCCTGCGTGGCTTTACCGCTGCGGCGCTGGAGTTGGGTACCACGCAGCCGGCAGTGAGCCAGCAGGTGAAGCGCCTGGAg                        <  2:392849/143‑1 (MQ=255)
                                                                                                                          ttATGTTTGAGCACCTTGCCGGGTTGTCGCTGGATACCTTGCGCGTATTCGAGGCTGCTGCGCGCCTGCGTGGCTTTACCGCTGCGGCGCTGGAGTTGGGTACCACGCAGCCGGCAGTGAGCCAGCAGGTGAAGCGCCTGGAg                        >  1:392849/1‑143 (MQ=255)
                                                                                                                             tGTTTGAGCACCTTGCCGGGTTGTCGCTGGATACCTTGCGCGTATTCGAGGCTGCTGCGCGCCTGCGTGGCTTTACCGCTGCGGCGCTGGAGTTGGGTACCACGCAGCCGGCAGTGAGCCAGCAGGTGAAGCGCCTGGAGGCACAGTTgg              <  2:151379/150‑1 (MQ=255)
                                                                                                                              gTTTGAGCACCTTGCCGGGTTGTCGCTGGATACCTTGCGCGTATTCGAGGCTGCTGCGCGCCTGCGTGGCTTTACCGCTGCGGCGCTGGAGTTGGGTACCACGCAGCCGGCAGTGAGCCAGCAGGTGAAGCGCCTGGAGGCACAGTTggg             <  2:378680/150‑1 (MQ=255)
                                                                                                                                        cTTGCCGGGTTGTCGCTGGATACCTTGCGCGTATTCGAGGCTGCTGCGCGCCTGCGTGGCTTTACCGCTGCGGCGCTGGAGTTGGGTACCACGCAGCCGGCAGTGAGCCAGCAGGTGAAGCGCCTGGAGGCACAGTTGGGCACGCGCCTg   >  2:41920/1‑150 (MQ=255)
                                                                                                                                         ttGCCGGGTTGTCGCTGGATACCTTGCGAGTATTCGAGGCTGCTGCGCGCCTGCGTGGGTTTACCGCTGCGGCGCTGGAGTTGGGTACCACGTTGCCCGCAGTGAGCCAGCAGGTAAAGAGGCTTGAGGCAAAGGTGCGCAcacgtagct  >  2:168417/1‑145 (MQ=255)
                                                                                                                                         |                                                                                                                                                     
TATTCGGACGCGTCATGGTGGCTTCCATCGTTGAGGGTTATGCGAACCGCTTGCCTACCAAGATGCGACTGTGGATAACATGGGTAAAGCCCATGGCGGGCAATGACTGGGATTAGCCAAGCTTATGTTTGAGCACCTTGCCGGGTTGTCGCTGGATACCTTGCGCGTATTCGAGGCTGCTGCGCGCCTGCGTGGCTTTACCGCTGCGGCGCTGGAGTTGGGTACCACGCAGCCGGCAGTGAGCCAGCAGGTGAAGCGCCTGGAGGCACAGTTGGGCACGCGCCTGT  >  NC_002947/89302‑89588

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑