Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	2,659,443	T→C	28.6%	Q312Q (CAA→CAG)	PP_2331 ←	membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	2,659,443	0	T	C	28.6%	14.3 / 5.2	14	Q312Q (CAA→CAG)	PP_2331	membrane protein
Reads supporting (aligned to +/- strand):  ref base T (5/5);  new base C (2/2);  total (7/7)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.96e-01

CGGAACGCCAGGGCGATCAGCACCGGGGTGAATGTGCCCAGGGCCTGGATGCCGATCAGGTTGCGCAGCACCAGGATCACCAGCACGCCGATCGGGATCATGACCATGATCATGAAGGTTTGCTGGGTTTGCAGTGGCAGGCCGTACAGCGAGTATTCGAGGAAGTCGGCGTCGGTGTTCTCGTCGGTCAGCTTGGCCAGGCGGATGGCGTTCATCTCGCTGTTGTTCATGCTGAAGGTGACGGTGGCCTTCTTGCCGCCAT  >  NC_002947/2659324‑2659585                                                                                                                        |                                                                                                                                               cgGAACGCCAGGGCGATCAGCACCGGGGTGAATGTGCCCAGGGCCTGGATGCCGATCAGGTTGCGCAGCACCAGGATCACCAGCACGCCGATCGGGATCATGACCATGATCATGAAGGTTTGCTGGGTTTGCAGTGGCAGGCCGTACAGc                                                                                                                  <  1:368468/150‑1 (MQ=255)                        cGGGGTGAATGTGCCCAGGGCCTGGATGCCGATCAGGTTGCGCAGCACCAGGATCACCAGCACGCCGATCGGGATCATGACCATGATCATGAAGGTTTGCTGGGTTTGCAGTGGCAGGCCGTACAGCGAGTATTCGa                                                                                                        <  1:80711/137‑1 (MQ=255)                        cGGGGTGAATGTGCCCAGGGCCTGGATGCCGATCAGGTTGCGCAGCACCAGGATCACCAGCACGCCGATCGGGATCATGACCATGATCATGAAGGTTTGCTGGGTTTGCAGTGGCAGGCCGTACAGCGAGTATTCGa                                                                                                        >  2:80711/1‑137 (MQ=255)                                             cTGGATGCCGATCAGGTTGCGCAGCACCAGGATCACCAGCACGCCGATCGGGATCATGACCATGATCATGAAGGTTTGCTGGGTTTGCAGTGGCAGGCCGTACAGCGAGTATTCGAGGAAGTCGGCGTCGGTGTTCTCGTCGGTCAGCtt                                                                      >  2:28144/1‑150 (MQ=255)                                                                       ccAGGATCACCAGCACGCCGATCGGGATCATGACCATGATCATGAAGGTTTGCTGGGTTTGCAGTGGCAGGCCGTACAGCGAGTATTCGAGGAAGTCGGCGTCGGTGTTCTCGTCGGTCAGCTTGGCCAgg                                                               <  1:432808/131‑1 (MQ=255)                                                                       ccAGGATCACCAGCACGCCGATCGGGATCATGACCATGATCATGAAGGTTTGCTGGGTTTGCAGTGGCAGGCCGTACAGCGAGTATTCGAGGAAGTCGGCGTCGGTGTTCTCGTCGGTCAGCTTGGCCAgg                                                               >  2:432808/1‑131 (MQ=255)                                                                          ggATCACCAGCACGCCGATCGGGATCATGACCATGATCATGAAGGTTTGCTGGGTTTGCAGTGGCAGGCCGTACAGCGAGTATTCGAGGAAGTCGGCGTCGGTGTTCTCGTCGGTCAGCTTGGCCAGGCGGATGGCGTTCATCTCGCtgt                                         >  1:382163/1‑150 (MQ=255)                                                                                       gCCGATCGGGATCATGACCATGATCATGAAGGTCTGCTGGGTTTGCAGTGGCAGGCCGTACAGCGAGTATTCGAGGAAGTCGGCGTCGGTGTTCTCGTCGGTCAGCTTGGcc                                                                  <  1:283636/112‑1 (MQ=255)                                                                                       gCCGATCGGGATCATGACCATGATCATGAAGGTCTGCTGGGTTTGCAGTGGCAGGCCGTACAGCGAGTATTCGAGGAAGTCGGCGTCGGTGTTCTCGTCGGTCAGCTTGGcc                                                                  <  1:61768/112‑1 (MQ=255)                                                                                       gCCGATCGGGATCATGACCATGATCATGAAGGTCTGCTGGGTTTGCAGTGGCAGGCCGTACAGCGAGTATTCGAGGAAGTCGGCGTCGGTGTTCTCGTCGGTCAGCTTGGcc                                                                  >  2:283636/1‑112 (MQ=255)                                                                                       gCCGATCGGGATCATGACCATGATCATGAAGGTCTGCTGGGTTTGCAGTGGCAGGCCGTACAGCGAGTATTCGAGGAAGTCGGCGTCGGTGTTCTCGTCGGTCAGCTTGGcc                                                                  >  2:61768/1‑112 (MQ=255)                                                                                         ccttcatgATCATGGTCATGATCATGAAGGTTTGCTGGGTTTGCAGTGGCAGGCCGTACAGCGAGTATTCGAGGAAGTCGGCGTCGGTGTTCTCGTCGGTCAGCTTGGCCAGGCGGATGGCGTTCATCTCGCTGTTGTTCATGCTGAAgg                          >  2:92096/8‑150 (MQ=255)                                                                                                 ttCATGACCATGATCATGAAGGTTTGTTGGGTTTGCAGTGGCAGGCCGTATAGCGAGTATTCGAGGAAGTCGGCGTCGGTTTTCTCTTCGGTCAGCTTGGCCAGGCGGATGGCGTTCATCTCGCTGTTGTTCATGCTGAAGGTGACGGTg                  <  2:122318/149‑1 (MQ=255)                                                                                                                 tGAAGGTTTGCTGGGTTTGCAGTGGCAGGCCGTACAGCGAGTATTCGAGGAAGTCGGCGTCGGTGTTCTCGTCGGTCAGCTTGGCCAGGCGGATGGCGTTCATCTCGCTGTTGTTCATGCTGAAGGTGACGGTGGCCTTCTTGCCGCCAt  <  1:28144/150‑1 (MQ=255)                                                                                                                        |                                                                                                                                               CGGAACGCCAGGGCGATCAGCACCGGGGTGAATGTGCCCAGGGCCTGGATGCCGATCAGGTTGCGCAGCACCAGGATCACCAGCACGCCGATCGGGATCATGACCATGATCATGAAGGTTTGCTGGGTTTGCAGTGGCAGGCCGTACAGCGAGTATTCGAGGAAGTCGGCGTCGGTGTTCTCGTCGGTCAGCTTGGCCAGGCGGATGGCGTTCATCTCGCTGTTGTTCATGCTGAAGGTGACGGTGGCCTTCTTGCCGCCAT  >  NC_002947/2659324‑2659585

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑