| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 2,670,426 | C→T | 25.0% | E645E (GAG→GAA) | acnB ← | aconitate hydratase B |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 2,670,426 | 0 | C | T | 25.0% | 22.6 / 4.6 | 16 | E645E (GAG→GAA) | acnB | aconitate hydratase B |
| Reads supporting (aligned to +/- strand): ref base C (5/7); new base T (2/2); total (7/9) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TCGTCCGGGTCGTTCGGCGCGCAGAGCACAGGCTCCTTGACGTCGGCCAGGTCGATTTCGATGATTTCGGCGTATTCGGCATCGGCGTCGGCCGACAGCAGCTCAGGCTTGGCCAGCCAGGCTTCCATGGCCTGGGCGCGGCGCTCCAGGGTGCGGGCATCGCCGTAGCCTTCGCCGATCATCCAGCGCAGCAGGGTGATGTTGGACTGCAGGTACTCGGCGATGGCCTTTTCCGGCAGCTTGATGGTGCAACCGGCAGCGGAACGCTCGGCCGAGGCATCGG > NC_002947/2670283‑2670565 | tCGTCCGGGTCGTTCGGCGCGCAGAGCACAGGCTCCTTGACGTCGGCCAGGTCGATTTCGATGATTTCGGCGTATTCGGCATCGGCGTCGGCCGACAGCAGCTCAGGCTTGGCCAGCCAGGCTTCCATGGCCTGGGCGCGGCGCTCCAgg > 1:270448/1‑150 (MQ=255)tCGTCCGGGTCGTTCGGCGCGCAGAGCACAGGCTCCTTGACGTCGGCCAGGTCGATTTCGATGACCCCGCGCTATTCGGCATCGGCGTCGGCCGACAGCAGCTCAGGCTTGGCCAGCCAGGCTTCCATGGCCTGGGCGCGGCGCTCCAgg < 1:140963/150‑1 (MQ=255) cgcAGAGCACAGGCTCCTTGACGTCGGCCAGGTCGATTTCGATGATTTCGGCGTATTCGGCATCGGCGTCGGCCGACAGCAGCTCAGGCTTGGCCAGCCAGGCTTCCATGGCCTGGGCGCGGCGCTCCCGGGTGCGGGCATCGCCGTAGc < 1:329100/150‑1 (MQ=255) gCTCCTTGACGTCGGCCAGGTCGATTTCGATGATTTCGGCGTATTCGGCATCGGCGTCGGCCGACAGCAGCTCAGGCTTGGCCAGCCAGGCTTCCATGGCCTGGGCGCGGCGCTCCAGGGTGCGGGCATCGCCGTAGCCTTCGCCGatca > 1:427986/1‑150 (MQ=255) ccTTGACGTCGGCCAGGTCGATTTCGATGATTTCGGCGTATTCGGCATCGGCGTCGGCCGACAGCAGCCCAGGCTTGGCCAGCCAGGCTTCCATGGCCTGGGCGCGGCGCTCCAGGGTGCGGGCATCGCCGTAGCCTTCGCCGATCATcc < 2:444113/150‑1 (MQ=255) ttGACGTCGGCCAGGTCGATTTCGATGATTTCGGCGTATTCGGCATCGGCGTCGGCCGACAGCAGCTCAGGCTTGGCCAGCCAGGCTTCCATGGCCTGGGCGCGGCGCTCCAGGGTGCGGGCATCGCCGTAGCCTTCGCCGATCATCCAg < 2:270448/150‑1 (MQ=255) ccAGGTCGATTTCGATGATTTCGGCGTATTCGGCATCGGCGTCGGCCGACAGCAGCTCAGGCTTGGCCAGCCAGGCTTCCATGGCCTGGGCGCGGCGCTCCAGGGTGCGGGCATCGCCGTa > 1:353582/1‑121 (MQ=255) ccAGGTCGATTTCGATGATTTCGGCGTATTCGGCATCGGCGTCGGCCGACAGCAGCTCAGGCTTGGCCAGCCAGGCTTCCATGGCCTGGGCGCGGCGCTCCAGGGTGCGGGCATCGCCGTa < 2:353582/121‑1 (MQ=255) gATTTCGATGATTTCGGCGTATTCGGCATCGGCGTCGGCCGACAGCAGCTCAGGCTTGGCCAGCCAGGCTTCCATGGCCTGGGCGCGGCGCTCCAGGGTGCGGGCATCGCCGTAGCCTTCGCCGATCATCCAGCGCAGCAGGGTGATGtt > 2:311605/1‑150 (MQ=255) gTCGGCCGACAGCAGCTCAGGCTTGGCCAGCCAGGCTTCCATGGCCTGGGCGCGGCGCTCCAGGGTGCGGGCATCGCCGTAGCCTTCGCCGATCATCCAGCGCAGCAGGGTGATGTTGGACTGCAGGTACTCGGCGATGGCCTTTTCCgg < 2:185971/150‑1 (MQ=255) cagcagCTCAGGCTTGGCCAGCCAGGCTTCCATGGCCTGGGCGCGGCGCTCCAGGGTGCGGGCATCGCCGTAGCCTTCGCCGATCATCCAGCGCAGCAGGGTGATGTTGGACTGCAGGTACTCGGCGATGGCCTTTTCCGGCAGCTTGAt < 1:311605/150‑1 (MQ=255) ccagccagGCTTCCATGGCCTGGGCGCGGCGTTCCAGGGTGCGGGCATCGCCGTAGCCTTCGCCGATCATCCAGCGCAGCAGGGTGATGTTGGACTGCAGGTACTCGGCGATGGCCTTTTCCGGCAGCTTGATGGTGCAACCGGCAGCgg > 2:365051/1‑150 (MQ=255) ccagccagGCTTCCATGGCCTGGGCGCGGCGTTCCAGGGTGCGGGCATCGCCGTAGCCTTCGCCGATCATCCAGCGCAGCAGGGTGATGTTGGACTGCAGGTACTCGGCGATGGCCTTTTCCGGCAGCTTGATGGTGCAACCGGCAGCgg > 2:365107/1‑150 (MQ=255) tGGGCGCGGCGTTCCAGGGTGCGGGCATCGCCGTAGCCTTCGCCGATCATCCAGCGCAGCAGGGTGATGTTGGACTGCAGGTACTCGGCGATGGCCTTTTCCGGCAGCTTGATGGTGCAACCGGCAGCGGAACGCTCGGCCGAGGCATCg < 1:365051/150‑1 (MQ=255) tGGGCGCGGCGTTCCAGGGTGCGGGCATCGCCGTAGCCTTCGCCGATCATCCAGCGCAGCAGGGTGATGTTGGACTGCAGGTACTCGGCGATGGCCTTTTCCGGCAGCTTGATGGTGCAACCGGCAGCGGAACGCTCGGCCGAGGCATCg < 1:365107/150‑1 (MQ=255) gggCGCGGCGCTCCAGGGTGCGGGCATCGCCGTAGCCTTCGCCGATCATCCAGCGCAGCAGGGTGATGTTGGACTGCAGGTACTCGGCGATGGCCTTTTCCGGCAGCTTGATGGTGCAACCGGCAGCGGAACGCTCGGCCGAGGCATCgg > 1:347574/1‑150 (MQ=255) | TCGTCCGGGTCGTTCGGCGCGCAGAGCACAGGCTCCTTGACGTCGGCCAGGTCGATTTCGATGATTTCGGCGTATTCGGCATCGGCGTCGGCCGACAGCAGCTCAGGCTTGGCCAGCCAGGCTTCCATGGCCTGGGCGCGGCGCTCCAGGGTGCGGGCATCGCCGTAGCCTTCGCCGATCATCCAGCGCAGCAGGGTGATGTTGGACTGCAGGTACTCGGCGATGGCCTTTTCCGGCAGCTTGATGGTGCAACCGGCAGCGGAACGCTCGGCCGAGGCATCGG > NC_002947/2670283‑2670565 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |