| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 3,105,530 | G→C | 26.8% | T22S (ACC→AGC) | arsH ← | ArsH protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 3,105,530 | 0 | G | C | 26.8% | 18.5 / 7.4 | 15 | T22S (ACC→AGC) | arsH | ArsH protein |
| Reads supporting (aligned to +/- strand): ref base G (3/8); new base C (2/2); total (5/10) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.60e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.24e-01 | |||||||||||
CGGGTTTCGGCACCGAAGTGCTCCAGGAGGCGCGCGGCTTCTTCTACCAGCAAGCGACTGAAGGAGCGTTCTCGGGTAGACCCGTACAGCAGCAGGATGCGAGGTTTGTGTTCGCAGGTCGGGGTTGCCGAAGGCGGCAGGTCGAACAGCGTGAGGTCGAGGTTAGGCAGTTGCTCGGACATGTTTTCTTCCTGTTCAAAGCGAGCCGATGCGATCCAGCGCGTGCTTGAGCTGCTCGCGGTCGAGGTGATCGAAC > NC_002947/3105413‑3105668 | cGGGTTTCGGCACCGAAGTGCTCCAGGAGGCGCGCGGCTTCTTCTACCAGCAAGCGACTGAAGGAGCGTTCTCGGGTAGACCCGTACAGCAGCAGGATGCGAGGTTTGTGTTCGCAGGTCGGGGTTGCCGAAGGCGGCAGGTCGAACAGc < 2:227497/150‑1 (MQ=255) gcgGCTTCTTCTACCAGCAAGCGACTGAAGGAGCGTTCTCGGGTAGACCCGTACAGCAGCAGGATGCGAGGTTTGTGTTCGCAGGTCGGGGTTGCCGAAGGCGGCAGGTCGAACAGCGTGAGGTCGAGGTTAGGCAGTTGCTCGGACATg > 1:211053/1‑150 (MQ=255) cttctAACAGCAAGCGACTGAAGAAGCGTTCTCGGGTAGACCCGTACAGCAGCAGGATGCGAGGTTTGTGTTCGCAGGTCGGGGTTGCCGAAGGCGGCAGGTCGAACAGCGTGAGGTCGAGGTTAGGCAGTTGCTCGGACATGTTttctt < 1:37850/150‑1 (MQ=255) tGAAGGAGCGTTCTCGGGTAGACCCGTACAGCAGCAGGATGCGAGGTTTGTGTTCGCAGGTCggg < 1:263571/65‑1 (MQ=255) tGAAGGAGCGTTCTCGGGTAGACCCGTACAGCAGCAGGATGCGAGGTTTGTGTTCGCAGGTCggg > 2:263571/1‑65 (MQ=255) aGCGTTCTCGGGTAGACCCGTACAGCAGCAGGATGCGAGGTTTGTGTTCGCAGGTCGGGGTTGCCGAAGGCGGCAGGTCGAACAGCGTGAGGTCGAGGTTAGGCAGTTGCTCGGACATGTTTTCTTCCTGTTCAAAGCGAGCCGATGCGa > 2:146011/1‑150 (MQ=255) gTAGACCCGTACAGCAGCAGGATGCGAGGTTTGTGTTCGCAGCTCGGGGTTGCCGAAGGCGGCAGGTCGAACAGCGt < 1:269425/77‑1 (MQ=255) gTAGACCCGTACAGCAGCAGGATGCGAGGTTTGTGTTCGCAGCTCGGGGTTGCCGAAGGCGGCAGGTCGAACAGCGt < 1:269546/77‑1 (MQ=255) gTAGACCCGTACAGCAGCAGGATGCGAGGTTTGTGTTCGCAGCTCGGGGTTGCCGAAGGCGGCAGGTCGAACAGCGt > 2:269425/1‑77 (MQ=255) gTAGACCCGTACAGCAGCAGGATGCGAGGTTTGTGTTCGCAGCTCGGGGTTGCCGAAGGCGGCAGGTCGAACAGCGt > 2:269546/1‑77 (MQ=255) gTACAGCAGCAGGATGCGAGGTTTGTGTTCGCAGGTCGGGGTTGCCGAAGGCGGCAGGTCGAACAGCGTGAGGTCGAGGTTAGGCAGTTGCTCGGACATGTTTTCTTCCTGTTCAAAGCGAGCCGATGCGATCCAGCGCGTGCTTGAgct < 2:211053/150‑1 (MQ=255) aCAGCAGCAGGATGCGAGGTTTGTGTTCGCAGGTCGGGGTTGCCGAAGGCGGCAGGTCGAACAGCGTGAGGTCGAGGTTAGGCAGTTGCTCGGACATGTTTTCTTCCTGTTCAAAGCGAGCCGATGCGATCCAGCGCGTGCTTGAgctgc < 1:182737/150‑1 (MQ=255) agcagGATGCGAGGTTTGTGTTCGCAGGTCGGGGTTGCCGAAGGCGGCAGGTCGAACAGCGTGAGGTCGAGGTTAGGCAGTTGCTCGGACATGTTTTCTTCCTGTTCAAAGCGAGCCGATGCGATCCAGCGCGTGCTTGAGCTGCTcgcg < 1:146011/150‑1 (MQ=255) tgtgTTCGCAGGTCGGGGTTGCCGAAGGCGGCAGGTCGAACAGCGTGAGGTCGAGGTTAGGCAGTTGCTCGGACATGTTTTCTTCCTGTTCAAAGCGAGCCGATGCGATCCAGCGCGTGCTTGAGCTGCTCGCGGTCGAGGTGATCGAAc < 1:226171/150‑1 (MQ=255) tgtgTTCGCAGGTCGGGGTTGCCGAAGGCGGCAGGTCGAACAGCGTGAGGTCGAGGTTAGGCAGTTGCTCGGACATGTTTTCTTCCTGTTCAAAGCGAGCCGATGCGATCCAGCGCGTGCTTGAGCTGCTCGCGGTCGAGGTGATCGAAc < 1:6254/150‑1 (MQ=255) | CGGGTTTCGGCACCGAAGTGCTCCAGGAGGCGCGCGGCTTCTTCTACCAGCAAGCGACTGAAGGAGCGTTCTCGGGTAGACCCGTACAGCAGCAGGATGCGAGGTTTGTGTTCGCAGGTCGGGGTTGCCGAAGGCGGCAGGTCGAACAGCGTGAGGTCGAGGTTAGGCAGTTGCTCGGACATGTTTTCTTCCTGTTCAAAGCGAGCCGATGCGATCCAGCGCGTGCTTGAGCTGCTCGCGGTCGAGGTGATCGAAC > NC_002947/3105413‑3105668 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |