Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	4,287,891	C→A	21.1%	R153L (CGT→CTT)	PP_3759 ←	CheB‑like methylesterase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	4,287,891	0	C	A	21.1%	33.2 / 4.6	19	R153L (CGT→CTT)	PP_3759	CheB‑like methylesterase
Reads supporting (aligned to +/- strand):  ref base C (5/10);  new base A (2/2);  total (7/12)
Fisher's exact test for biased strand distribution p-value = 6.03e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.38e-01

TCAGCAGTTTGGCGGTGGTGTGGCTTAGCATGCGCTGTATTCCAGGGTCGCGAGCAACTGCCCGATACCGCTCAGAGTAAGGATATGGTCGGGCTGGTGCAGGGCCAGGGCGGCCTCCGGCATCAGGGCAACCTGTGCGTCACGAGGGTCCTGGACGATCGTGCGCCCCCCATTGTTCTTGATGTAGGCAAGGCCCCTGGCGCCGTCTTCGTTGGCCCCGGTGAGCAGCACACCCAGCAGGCCATCGCCATAGGCATCGGCCGCCGAGATGAACAGGAAATCGATC  >  NC_002947/4287749‑4288034                                                                                                                                               |                                                                                                                                                tCAGCAGTTTGGCGGTGGTGTGGCTTAGCATGCGCTGTATTCCAGGGTCGCGAGCAACTGCCCGATACCGCTCAGAGTAAGGATATGGTCGGGCTGGTGCAGGGCCAGGGCGGCCTCCGGCATCAGGGCAACCTGTGCGTCACGAGGGTc                                                                                                                                          >  1:44986/1‑150 (MQ=255)               gtggtgTGGCTTAGCATGCGCTGTATTCCAGGGTCGCGAGCAACTGCCCGATACCGCTCAGAGTAAGGATATGGTCGGGCTGGTGCAGGGCCAGGGCGGCCTCCGGCATCAGGGCAACCTGTGCGTCACGAGGGTCCTGGACGATCGTgc                                                                                                                            <  2:144305/150‑1 (MQ=255)                               tGCGCTGTATTCCAGGGTCGCGAGCAACTGCCCGATACCGCTCAGAGTAAGGATATGGTCGGGCTGGTGCAGGGCCAGGGCGGCCTCCGGCATCAGGGCAACCTGTGCGTCACGAGGGTCCTGGACGATCGTGCGCCCCCCATTGTTCtt                                                                                                            >  2:367141/1‑150 (MQ=255)                                 cgcTGTATTCCAGGGTCGCGAGCAACTGCCCGATACCGCTCAGAGTAAGGATATGGTCGGGCTGGTGCAGGGCCAGGGCGGCCTCCGGCATCAGGGCAACCTGTGCGTCACGAGGGTCCTGGACGATCGTGCGCCCCCCATTGTTCTTGa                                                                                                          <  1:170708/150‑1 (MQ=255)                                    tGTATTCCAGGGTCGCGAGCAACTGCCCGATACCGCTCAGAGTAAGGATATGGTCGGGCTGGTGCAGGGCCAGGGCGGCCTCCGGCATCAGGGCAACCTGTGCGTCACGAGGGTCCTGGACGATCGTGCGCCCCCCATTGTTCTTGATGt                                                                                                       <  2:244177/150‑1 (MQ=255)                                            aGGGTCGCGAGCAACTGCCCGATACCGCTCAGAGTAAGGATATGGTCGGGCTGGTGCAGGGCCAGGGCGGCCTCCGGCATCAGGGCAACCTGTGCGTCACGAGGGTCCTGGACGATCGTGCGCCCCCCATTGTTCTTGATGTAGGCAAgg                                                                                               <  2:44986/150‑1 (MQ=255)                                               gTCGCGAGCAACTGCCCGATACCGCTCAGAGTAAGGATATGGTCGGGCTGGTGCAGGGCCAGGGCGGCCTCCGGCATCAGGGCAACCTGTGCGTCACGAGGGTCCTGGACGATCGTGCGCCCCCCATTGTTCTTGATGTAGGCAAGGccc                                                                                            <  1:30255/150‑1 (MQ=255)                                                             cccGATACCGCTCAGAGTAAGGATATGGTCGGGCTGGTGCAGGGCCAGGGCGGCCTCCGGCATCAGGGCAACCTGTGCGTCACGAGGGTCCTGGACGATCGTGCGCCCCCCATTGTTCTTGATGTAGGCAAGGCCCCTGGCGCCGTCTTc                                                                              >  1:362525/1‑150 (MQ=255)                                                                gATACCGCTCAGAGTAAGGATATGGTCGGGCTGGTGCAGGGCCAGGGCGGCCTCCGGCATCAGGGCAACCTGTGCGTCACGAGGGTCCTGGACGATCGTGCGCCCCCCATTGTTCTTGATGTAGGCAAGGCCCCTGGCGCCGTCTTCGtt                                                                           >  2:325820/1‑150 (MQ=255)                                                                gATACCGCTCAGAGTAAGGATATGGTCGGGCTGGTGCAGGGCCAGGGCGGCCTCCGGCATCAGGGCAACCTGTGCGTCACGAGGGTCCTGGACGATCGTGCGCCCCCCATTGTTCTTGATGTAGGCAAGGCCCCTGGCGCCGTCTTCGtt                                                                           <  2:420656/150‑1 (MQ=255)                                                                                           gggCTGGTGCAGGGCCAGGGCGGCCTCCGGCATCAGGGCAACCTGTGCGTCACGAGGGTCCTGGACGATCGTGCGCCCCCCATTGTTCTTGATGTAGGCAAGGCCCCTGGCGCCGTCTTCGTTGGCCCCGGTGAGCAGCACACCcagcag                                                <  2:2611/150‑1 (MQ=255)                                                                                               tGGTGCAGGGCCAGGGCGGCCTCCGGCATCAGGGCAACCTGTGCGTCAAGAGGGTCCTGGACGATCGTGCGCCCCCCATTGTTCTTGATGTAGGCAAGGCCCCTGGCGCCGTCTTCGTTGGCCCCGGTGAGCAGCACACCCAGCAGGCCa                                            >  1:374432/1‑150 (MQ=255)                                                                                               tGGTGCAGGGCCAGGGCGGCCTCCGGCATCAGGGCAACCTGTGCGTCAAGAGGGTCCTGGACGATCGTGCGCCCCCCATTGTTCTTGATGTAGGCAAGGCCCCTGGCGCCGTCTTCGTTGGCCCCGGTGAGCAGCACACCCAGCAGGCCa                                            >  1:374422/1‑150 (MQ=255)                                                                                                        gccagggcGGCCTCCGGCATCAGGGCAACCTGTGCGTCACGAGGGTCCTGGACGATCGTGCGCCCCCCATTGTTCTTGATGTAGGCAAGGCCCCTGGCGCCGTCTTCGTTGGCCCCGGTGAGCAGCACACCCAGCAGGCCATCGCCATAg                                   <  1:325820/150‑1 (MQ=255)                                                                                                          cagggcGGCCTCCGGCATCAGGGCAACCTGTGCGTCACGAGGGTCCTGGACGATCGTGCGCCCCCCATTGTTCTTGATGTAGGCAAGGCCCCTGGCGCCGTCTTCGTTGGCCCCGGTGAGCAGCACACCCAGCAGGCCATCGCCATAGGc                                 >  1:94034/1‑150 (MQ=255)                                                                                                                    tCCGGCATCAGGGCAACCTGTGCGTCACGAGGGTCCTGGACGATCGTGCGCCCCCCATTGTTCTTGATGTAGGCAAGGCCCCTGGCGCCGTCTTCGTTGGCCCCGGTGAGCAGCACACCCAGCAGGCCATCGCCATAGGCATCGgccgcc                       <  2:412592/150‑1 (MQ=255)                                                                                                                        gCATCAGGGCAACCTGTGCGTCACGAGGGTCCTGGACGATCGTGCGCCCCCCATTGTTCTTGATGTAGGCAAGGCCCCTGGCGCCGTCTTCGTTGGCCCCGGTGAGCAGCACACCCAGCAGGCCATCGCCATAGGCATCGGCCGCCgaga                   <  1:261536/150‑1 (MQ=255)                                                                                                                                         gCGTCAAGAGGGTCCTGGACGATCGTGCGCCCCCCATTGTTCTTGATGTAGGCAAGGCCCCTGGCGCCGTCTTCGTTGGCCCCGGTGAGCAGCACACCCAGCAGGCCATCGCCATAGGCATCGGCCGCCGAGATGAACAGGAAatcgatc  <  2:374422/150‑1 (MQ=255)                                                                                                                                         gCGTCAAGAGGGTCCTGGACGATCGTGCGCCCCCCATTGTTCTTGATGTAGGCAAGGCCCCTGGCGCCGTCTTCGTTGGCCCCGGTGAGCAGCACACCCAGCAGGCCATCGCCATAGGCATCGGCCGCCGAGATGAACAGGAAatcgatc  <  2:374432/150‑1 (MQ=255)                                                                                                                                               |                                                                                                                                                TCAGCAGTTTGGCGGTGGTGTGGCTTAGCATGCGCTGTATTCCAGGGTCGCGAGCAACTGCCCGATACCGCTCAGAGTAAGGATATGGTCGGGCTGGTGCAGGGCCAGGGCGGCCTCCGGCATCAGGGCAACCTGTGCGTCACGAGGGTCCTGGACGATCGTGCGCCCCCCATTGTTCTTGATGTAGGCAAGGCCCCTGGCGCCGTCTTCGTTGGCCCCGGTGAGCAGCACACCCAGCAGGCCATCGCCATAGGCATCGGCCGCCGAGATGAACAGGAAATCGATC  >  NC_002947/4287749‑4288034

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑