| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 5,329,444 | T→A | 21.8% | L10Q (CTG→CAG) | PP_4690 → | Rieske 2Fe‑2S family protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 5,329,444 | 0 | T | A | 21.8% | 41.1 / 10.0 | 23 | L10Q (CTG→CAG) | PP_4690 | Rieske 2Fe‑2S family protein |
| Reads supporting (aligned to +/- strand): ref base T (10/8); new base A (3/2); total (13/10) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.05e-01 | |||||||||||
CTTGTGGCGAGCGGCGGAACTGCGCACCATAGGCAGCCGGGCGCAGAGAATGCGATCAATTTGATAATTATTCGCATTGGAGCGTCAAGCCATCACGTGTTTCACGGGTCATCCTTGCCATGCACTTCCTTTGTACATCCCTCGGCCTGGCCGAGGGCCAAAGCCGCGCCTTCAGCGTAGACGGCCTCGAACTGTTCGGCGTGCGTCGCCAAGGCCAGGTGTACCTGTACCGCAACCGCTGCCCGCACCGGGACATCCCGCTGAACTGGGCAGAGGACGCGTTTCTGGATGA > NC_002947/5329297‑5329588 | cTTGTGGCGAGCGGCGGAACTGCGCACCATAGGCAGCCGGGCGCAGAGAATGCGATCAATTTGATAATTATTCGCATTGGAGCGTCAAGCCATCACGTGTTTCACGGGTCATCCTTGCCATGCACTTCCTTTGTACATCCCTCGGCCTgg > 1:50268/1‑150 (MQ=255) ccgctctcgcACCATAGTCAGCCGGTCGCAGAGAATGCGATCAATTTGATAATTATTCGCATTGGAGCGTCAAGCCATTACGTGTTTCACGGGTCATCCTTGCCATGCACTTCTTGTGTACATCCCTCGGCCTGGCCGAGGGCCAAAGcc < 1:208951/143‑1 (MQ=255) aCTGCGCACCATAGGCAGCCGGGCGCAGAGAATGCGATCAATTTGATAATTATTCGCATTGGAGCGTCAAGCCATCACGTGTTTCACGGGTCATCCTTGCCATGCACTTCCTTTGTACATCCCTCGGCCTGGCCGAGGGCCAAAGCcgcg > 1:130214/1‑150 (MQ=255) gcACCATAGGCAGCCGGGCGCAGAGAATGCGATCAATTTGATAATTATTCGCATTGGAGCGTCAAGCCATCACGTGTTTCACGGGTCATCATTGCCATGAACTTCCTTTGTACATCCCTCTGTCTGGCCGAGGGCCAAAGCcgagattgg > 2:208951/1‑143 (MQ=255) gCAGCCGGGCGCAGAGAATGCGATCAATTTGATAATTATTCGCATTGGAGCGTCAAGCCATCACGTGTTTCACGGGTCATCCTTGCCATGCACTTCCTTTGTACATCCCTCGGCCTGGCCGAGGGCCAAAGCCACGCCTTCAGCGTAGAc > 2:441803/1‑150 (MQ=255) aGCCGGGCGCAGAGAATGCGATCAATTTGATAATTATTCGCATTGGAGCGTCAAGCCATCACGTGTTTCACGGGTCATCCTTGCCATGCACTTCCTTTGTACATCCCTCGGCCTGGCCGAGGGCCAAAGCCACGCCTTCAGCGTAGACgg < 1:441803/150‑1 (MQ=255) gCGATCAATTTGATAATTATTCGCATTGGAGCGTCAAGCCATCACGTGTTTCACGGGTCATCCTTGCCATGCACTTCCTTTGTACATCCCTCGGCCTGGCCGAGGGCCAAAGCCGCGCCTTCAGCGTAGACGGCCTCGCACttttaagcg < 2:130214/150‑6 (MQ=255) attattCGCATTGGAGCGTCAAGCCATCACGTGTTTCACGGGTCATCCTTGCCATGCACTTCCTTTGTACATCCCTCGGCCTGGCCGAGGGCCAAAGCCGCGCCTTCAGCGTAGACGGCCTCGAACTGTTCGGCGTGCGTCGCCAAGGcc < 1:82339/150‑1 (MQ=255) aTTGGAGCGTCAAGCCATCACGTGTTTCACGGGTCATCCTTGCCATGCACTTCCTTTGTACATCCCTCGGCCTGGCCGAGGGCCAAAGCCGCGCCTTCAGCGTAGACGGCCTCGAACTGTTCGGCGTGCGTCGCCAAGGCCAGGtgtacc > 1:380607/1‑150 (MQ=255) ttGGAGCGTCAAGCCATCACGTGTTTCACGGGTCATCCTTGCCATGCACTTCCTTTGTACATCCCTCGGCCTGGCCGAGGGCCAAAGCCGCGCCTTCAGCGTAGACGGCCTCGAACTGTTCGGCGTGCGTCGCCAAGGCCAGGtgtacct > 2:255810/1‑150 (MQ=255) aaGCCATCACGTGTTTCACGGGTCATCCTTGCCATGCACTTCCTTTGTACATCCCTCGGCCTGGCCGAGGGCCAAAGCCGCGCCTTCAGCGTAGACGGCCTCGAACTGTTCGGCGTGCGTCGCCAAGGCCAGGtgtac > 1:42573/1‑138 (MQ=255) aaGCCATCACGTGTTTCACGGGTCATCCTTGCCATGCACTTCCTTTGTACATCCCTCGGCCTGGCCGAGGGCCAAAGCCGCGCCTTCAGCGTAGACGGCCTCGAACTGTTCGGCGTGCGTCGCCAAGGCCAGGtgtac < 2:42573/138‑1 (MQ=255) tCACGTGTTTCACGGGTCATCCTTGCCATGCACTTCCTTTGTACATCCCTCGGCCTGGCCGAGGGCCAAAGCCGCGCCTTCAGCGTAGACGGCCTCGAACTGTTCGGCGTGCGTCGCCAAGGCCAGGTGTACCTGTACCGCAACCGCTGc > 2:266025/1‑150 (MQ=255) tCACGGGTCATCCTTGCCATGCACTTCCTTTGTACATCCCTCGGCCTGGCCGAGGGCCAAAGCCGCGCCTTCAGCGTAGACGGCCTCGAACTGTTCGGCGTGCGTCGCCAAGGCCAGGTGTACCTGTACCGCAACCGCTGCCCGCACCgg < 1:266025/150‑1 (MQ=255) tCCTTGCCATGCACTTCCTTTGTACATCCCTCGGCCTGGCCGAGGGCCAAAGCCGCGCCTTCAGCGTAGACGGCCTCGAACTGTTCGGCGTGCGTCGCCAAGGCCAGGTGTACCTGTACCGCAACCGCTGCACGCACCGGGACATCCCGc > 2:234662/1‑150 (MQ=255) ccTTGCCATGCACTTCCTTTGTACATCCCTCGGCCTGGCCGAGGGCCAAAGCCGCGCCTTCAGCGTAGACGGCCTCGAACTGTTCGGCGTGCGTCGCCAAGGCCAGGTGTACCTGTACCGCAACCGCTGCCCGCACCGGGACATCCCGCt < 2:380607/150‑1 (MQ=255) gTACATCCCTCGGCCTGGCCGAGGGCCAAAGCCGCGCCTTCAGCGTAGACGGCCTCGAACTGTTCGGCGTGCGTCGCCAAGGCCAGGTGTACCTGTACCGCAACCGCTGCCCGCACCGGGACATCCCGCTGAACTGGGCAGAGGACGCGt < 1:234662/150‑1 (MQ=255) ttggcccTCGGCCAGGCCGAGGGCCAAAGCCGCGCCTTCAGCGTAGACGGCCTCGAACTGTTCGGCGTGCGTCGCCAAGGCCAGGTGTACCTGTACCGCAACCGCTGCCCGCACCGGGACATCCCGCTGAACTGGGCAGAGGACGCGttt > 2:391450/5‑150 (MQ=255) tggcccTCGGCCAGGCCGAGGGCCAAAGCCGCGCCTTCAGCGTAGACGGCCTCGAACTGTTCGGCGTGCGTCGCCAAGGCCAGGTGTACCTGTACCGCAACCGCTGCCCGCACCGGGACATCCCGCTGAACTGGGCAGAGGACGCGTTTc > 2:133922/4‑150 (MQ=255) cccTCGGCCTGGCCGAGGGCCAAAGCCGCGCCTTCAGCGTAGACGGCCTCGAACTGTTCGGCGTGCGTCGCCAAGGCCAGGTGTACCTGTACCGCAACCGCTGCCCGCTCTGCGACATCCCTCTGAACTGGGCAGAGGACGCGTTTCTgg > 2:140594/1‑150 (MQ=255) ccTCGGCCAGGCCGAGGGCCAAAGCCGCGCCTTCAGCGTAGACGGCCTCGAACTGTTCGGCGTGCGTCGCCAAGGCCAGGTGTACCTGTACCGCAACCGCTGCCCGCACCGGGACATCCCGCt < 1:210008/123‑1 (MQ=255) ccTCGGCCAGGCCGAGGGCCAAAGCCGCGCCTTCAGCGTAGACGGCCTCGAACTGTTCGGCGTGCGTCGCCAAGGCCAGGTGTACCTGTACCGCAACCGCTGCCCGCACCGGGACATCCCGCt > 2:210008/1‑123 (MQ=255) cGGCCAGGCCGAGGGCCAAAGCCGCGCCTTCAGCGTAGACGGCCTCGAACTGTTCGGCGTGCGTCGCCAAGGCCAGGTGTACCTGTACCGCAACCGCTGCCCGCACCGGGACATCCCGCTGAACTGGGCAGAGGACGCGTTTCTGGATGa < 1:391450/150‑1 (MQ=255) | CTTGTGGCGAGCGGCGGAACTGCGCACCATAGGCAGCCGGGCGCAGAGAATGCGATCAATTTGATAATTATTCGCATTGGAGCGTCAAGCCATCACGTGTTTCACGGGTCATCCTTGCCATGCACTTCCTTTGTACATCCCTCGGCCTGGCCGAGGGCCAAAGCCGCGCCTTCAGCGTAGACGGCCTCGAACTGTTCGGCGTGCGTCGCCAAGGCCAGGTGTACCTGTACCGCAACCGCTGCCCGCACCGGGACATCCCGCTGAACTGGGCAGAGGACGCGTTTCTGGATGA > NC_002947/5329297‑5329588 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |