Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,803,360 A→G 16.0% M165T (ATG→ACG)  PP_5081 ← type IV pili biogenesis protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,803,3600AG16.0% 51.9 / 4.2 25M165T (ATG→ACG) PP_5081type IV pili biogenesis protein
Reads supporting (aligned to +/- strand):  ref base A (8/13);  new base G (2/2);  total (10/15)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.78e-01

GTCCACTTCAGGCGCAGCCATTGCGGCCCTTGCCGGCAGGTGCGCCAGCATTGCAGG‑TGCCTTCACGGGCGTAGCCGTGTGATAGGCATCAACCCGCAACTGCAGGCGCAACAGTCCAGGCTGGCGATCAACGGTTGCCAGGCTCATGTCACCGCTGCGCAGAATCCGTGCCTGCCCTAACCAGTCGTCCAGCCACAGCCGCAAGGCCGGATAACGCCCCACCACTTGCACCACCAGCGGTACCTTGCTGAATCCGGCCTGTTGCTCACCTGCCTCTACATCGAGGCGTTCGAC  >  NC_002947/5803215‑5803508
                                                                                                                                                  |                                                                                                                                                    
gTCCACTTCAGGCGCAGCCATTGCGGATCACTCCGGCAGGTGCGCCAGCATTGCAGG‑TGCCTTCACGGGCGTAGCCGTGTGATAGGCATCAACCCGCAACTACAGGCGCAACAGTCCAGGCTGGCGATCAACGGTTGCCAGGCTCATGTc                                                                                                                                                  >  1:11509/1‑150 (MQ=255)
           gcgcAGCCATTGCGGCCCTTGCCGGCAGGTGCGCCAGCATTGCAGGATGAC‑AATCAGGCGTAGCCGTGTGATAGGCATCAACCCGCAACTACAGGCGCAACAGTCCAGGCTGGCGATCAACGGTTGCCAGGCTCATGTCACCGCTGCGCa                                                                                                                                       <  2:11509/150‑1 (MQ=255)
               aGCCATTGCGGCCCTTGCCGGCAGGTGCGCCAGCATTGCAGG‑TGCCTTCACGGGCGTATGCGTGTGATAGGCCTCAACCCGCAACTGCAGGGGCAACAGTCCCGCCATGCGATCAACGGTTGCCAGGCACATGTCACCGCTGCGCAGCAt                                                                                                                                   >  1:138547/1‑150 (MQ=255)
                gCCATTGCGGCCCTTGCCGGCAGGTGCGCCAGCATTGCAGG‑TGCCTTCACGGGCGTAGCCGTGTGATAGGCATCAACCCGCAACTGCAGGCGCAACAGTCCAGGCTGGCGATCAACGGTTGCCAGGCTCATGTCACCGCTGCGCAGAATc                                                                                                                                  <  1:241843/150‑1 (MQ=255)
                                ccGGCAGGTGCGCCAGCATTGCAGG‑TGCCTTCACGGGCGTAGCCGTGTGATAGGCATCAACCCGCAACTGCAGGCGCAACAGTCCAGGCTGGCGATCAACGGTTGCCAGGCTCATGTCACCGCTGCGCAGAATCCGTGCCTGCCCTAAcc                                                                                                                  >  2:130190/1‑150 (MQ=255)
                                    cAGGCGCCCAAGCATTGCAGG‑TGCCTTACCGTGCGTAGCCGTGTGATAGGCATCAACCCGCAACTGCAGGCGCAACAGTCCAGGCTGGCGATCAACGGTTGCCAGGCTCATGTCACCGCTGCTCAGAATCCGTGCCTGCCCTAACCAgtc                                                                                                              <  2:138547/150‑1 (MQ=255)
                                     aGGTGCGCCAGCATTGCAGG‑TGCCTTCACGGGCGTAGCCGTGTGATAGGCATCAACCCGCAACTGCAGGCGCAACAGTCCAGGCTGGCGATCAACGGTTGCCAGGCTCATGTCACCGCTGCGCAGAATCCGTGCCTGCCCTAACCAg                                                                                                                >  2:198298/1‑147 (MQ=255)
                                     aGGTGCGCCAGCATTGCAGG‑TGCCTTCACGGGCGTAGCCGTGTGATAGGCATCAACCCGCAACTGCAGGCGCAACAGTCCAGGCTGGCGATCAACGGTTGCCAGGCTCATGTCACCGCTGCGCAGAATCCGTGCCTGCCCTAACCAg                                                                                                                <  1:198298/147‑1 (MQ=255)
                                       gTGCGCCAGCATTGCAGG‑TGCCTTCACGGGCGTAGCCGTGTGATAGGCATCAACCCGCAACTGCAGGCGCAACAGTCCAGGCTGGCGATCAACGGTTGCCAGGCTCATGTCACCGCTGCGCAGAATCCGTGCCTGCCCTAACCAgtcgtc                                                                                                           <  1:130190/150‑1 (MQ=255)
                                              aGCATTGCAGG‑TGCCTTCACGGGCGTAGCCGTGTGATAGGCATCAACCCCAAAATGCAGGCGCAACAGTCCAGGCTGGCGATCAACGGTTGCCAGGCTCGTGTCACCGCTGCGCAGAATCCGTGCCTGCCCTAACCATTCGTCCAGCcac                                                                                                    >  2:109489/1‑150 (MQ=255)
                                                        g‑TGCCTTCACGGGCGTAGCCGTGTGATAGGCATCAACCCGCAACTGCAGGCGCAACAGTCCAGGCTGGCGATCAACGGTTGCCAGGCTCATGTCACCGCTGCGCAGAATCCGTGCCTGCCCTAACCAGTCGTCCAGCCACAGCCGCAAgg                                                                                          <  1:108997/150‑1 (MQ=255)
                                                                                 gATAGGCATCAACCCGCAACTGCAGGCGCAACAGTCCAGGCTGGCGATCAACGGTTGCCAGGCTCATGTCACCGCTGCGCAGAATCCGTGCCTGCCCTAACCAGTCGTCCAGCCACAGCCGCAAGGCCGGATAACGCCCCACCACTTGca                                                                  <  1:328626/150‑1 (MQ=255)
                                                                                 gATAGGCATCAACCCGCAACTGCAGGCGCAACAGTCCAGGCTGGCGATCAACGGTTGCCAGGCTCATGTCACCGCTGCGCAGAATCCGTGCCTGCCCTAACCAGTCGTCCAGCCACAGCCGCAAGGCCGGATAACGCCCCACCACTTGca                                                                  >  1:349247/1‑150 (MQ=255)
                                                                                  aTAGGCATCAACCCGCAACTGCAGGCGCAACAGTCCAGGCTGGCGATCAACGGTTGCCAGGCTCATGTCACCGCTGCGCAGAATCCGTGCCTGCCCTAACCAGTCGTCCAGCCACAGCCGCAAGGCCGGATAACGCCCCACCACTTGcac                                                                 >  2:265042/1‑150 (MQ=255)
                                                                                   tAGGCATCAACCCGCAACTGCAGGCGCAACAGTCCAGGCTGGCGATCAACGGTTGCCAGGCTCATGTCACCGCTGCGCAGAATCCGTGCCTGCCCTAACCAGTCGTCCAGCCACAGCCGCAAGGCCGGATAACGCCCCACCACTTGcacc                                                                >  1:216073/1‑150 (MQ=255)
                                                                                      gCATCAACCCGCAACTGCAGGCGCAACAGTCCAGGCTGGCGATCAACGGTTGCCAGGCTCGTGTCACCGCTGCGCAGAATCCGTGCCTGCCCTAACCAGTCGTCCAGCCACAGCCGCAAGGCCGGATAACGCCCCACCACTTGCaccacc                                                             >  1:301975/1‑150 (MQ=255)
                                                                                                    cTGCAGGCGCAACAGTCCAGGCTGGCGATCAACGGTTGCCAGGCTCATGTCACCGCTGCGCAGAATCCGTGCCTGCCCTAACCAGTCGTCCAGCCACAGCCGCAAGGCCGGATAACGCCCCACCACTTGCACCACCAGCGGTACCTTGCt                                               <  2:216073/150‑1 (MQ=255)
                                                                                                         ggCGCAACAGTCCAGGCTGGCGATCAACGGTTGCCAGGCTCATGTCACCGCTGCGCAGAAGCCGTGCCTGCCCTAACCAGTCGTCCAGCCACAGCCGCAAGGCCGGATAACGCCCCACCACTTGCACCACCAGCGGTACCTTGCTGAATc                                          <  1:4729/150‑1 (MQ=255)
                                                                                                            gaaaCAGTCCAGGCTGGCGATCAACGGTTGCCAGGCTCATGTCACCGCTGCGCAGAATCCGTGCCTGCCCTAACCAGTCGTCCAGCCACAGCCGCAAGGCCGGATAACGCCCCACCACTTGCACCACCAGCGGTACCTTGCTGAATCCgg                                       <  1:159915/148‑1 (MQ=255)
                                                                                                             cAACAGTCCAGGCTGGCGATCAACGGTTGCCAGGCTCATGTCACCGCTGCGCAGAATCCGTGCCTGCCCTAACCAGTCGTCCAGCCACAGCCGCAAGGCCGGATAACGCCCCACCACTTGCACCACCAGCGGTACCTTGCTGAATCCGGc                                      <  1:66135/150‑1 (MQ=255)
                                                                                                                       ggCTGGCGATCAACGGTTGCCAGGCTCATGTCACCGCTGCGCAGAATCCGTGCCTGCCCTAACCAGTCGTCCAGCCACAGCCGCAAGGCCGGATAACGCCCCACCACTTGCACCACCAGCGGTACCTTGCTGAATCCGGCCTGTTGCTCa                            <  2:341657/150‑1 (MQ=255)
                                                                                                                                tCAACGGTTGCCAGGCTCATGTCACCGCTGCGCAGAATCCGTGCCTGCCCTAACCAGTCGTCCAGCCACAGCCGCAAGGCCGGATAACGCCCCACCACTTGCACCACCAGCGGTACCTTGCTGAATCCGGCCTGTTGCTCACCTGCctct                   >  2:400881/1‑150 (MQ=255)
                                                                                                                                       ttGCCAGGCTCGTGTCACCGCTGCGCAGAATCCGTGCCTGCCCTAACCAGTCGTCCAGCCACAGCCGCAAGGCCGGATAACGCCCCACCACTTGCACCACCAGCGGTACCTTGCTGAATCCGGCCTGTTGCTCACCTGCCTCTACATCGa            <  2:301975/150‑1 (MQ=255)
                                                                                                                                          ccAGGCTCGTGTCACCGCTGCGCAGAATCCGTGCCTGCCCTAACCATTCGTCCAGCCACAGCCGCAAGGCCGGATAACGCCCCACCACTTGCACCACCAGCGGTACCTTGCTGAATCCGGCCTGTTGCTCACCTGCCTCTACATCGAGGc         <  1:109489/150‑1 (MQ=255)
                                                                                                                                                 cATGTCACCGCTGCGCAGAATCCGTGCCTGCCCTAACCAGTCGTCCAGCCACAGCCGCAAGGCCGGATAACGCCCCACCACTTGCACCACCAGCGGTACCTTGCTGAATCCGGCCTGTTGCTCACCTGCCTCTACATCGAGGCGTTCGAc  <  1:265042/150‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                    
GTCCACTTCAGGCGCAGCCATTGCGGCCCTTGCCGGCAGGTGCGCCAGCATTGCAGG‑TGCCTTCACGGGCGTAGCCGTGTGATAGGCATCAACCCGCAACTGCAGGCGCAACAGTCCAGGCTGGCGATCAACGGTTGCCAGGCTCATGTCACCGCTGCGCAGAATCCGTGCCTGCCCTAACCAGTCGTCCAGCCACAGCCGCAAGGCCGGATAACGCCCCACCACTTGCACCACCAGCGGTACCTTGCTGAATCCGGCCTGTTGCTCACCTGCCTCTACATCGAGGCGTTCGAC  >  NC_002947/5803215‑5803508

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑